Publications by authors named "Wang Feng"

5,208 Publications

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Particle-based calculation and visualization of protein cavities using SES models.

IEEE J Biomed Health Inform 2021 Nov 29;PP. Epub 2021 Nov 29.

The analysis of molecular cavities, where ligands interact with protein structures, plays a critical role in protein structure-based drug design. However, it is a challenge because of the ambiguous definition of the cavity boundaries in most cavity detection methods. The cavities are mostly calculated by input parameters, which are difficult for users to visualize cavities in interactive ways. In this paper, we propose a novel method for the interactive exploration of cavity calculation and visualization. Firstly, the proposed method combines the two solvent-excluded surfaces (SES) models of a given protein to define the boundaries and provides cavity emission points. Secondly, the system provides a user-guided interactive method to allow users to select cavities by simply clicking operations and to track the cavity identify and filling process based on position constraints. Finally, the selected cavities are represented with the colorful depth perception method. Experiments show that our work can effectively identify and calculate cavities.
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http://dx.doi.org/10.1109/JBHI.2021.3130897DOI Listing
November 2021

Gels as emerging anti-icing materials: a mini review.

Mater Horiz 2021 Nov 29;8(12):3266-3280. Epub 2021 Nov 29.

NTNU Nanomechanical Lab, Department of Structural Engineering, Norwegian University of Science and Technology (NTNU), Trondheim 7491, Norway.

Gel materials have drawn great attention recently in the anti-icing research community due to their remarkable potential for reducing ice adhesion, inhibiting ice nucleation, and restricting ice propagation. Although the current anti-icing gels are in their infancy and far from practical applications due to poor durability, their outstanding prospect of icephobicity has already shed light on a new group of emerging anti-icing materials. There is a need for a timely review to consolidate the new trends and foster the development towards dedicated applications. Starting from the stage of icing, we first survey the relevant anti-icing strategies. The latest anti-icing gels are then categorized by their liquid phases into organogels, hydrogels, and ionogels. At the same time, the current research focuses, anti-icing mechanisms and shortcomings affiliated with each category are carefully analysed. Based upon the reported state-of-the-art anti-icing research and our own experience in polymer-based anti-icing materials, suggestions for the future development of the anti-icing gels are presented, including pathways to enhance durability, the need to build up the missing fundamentals, and the possibility to enable stimuli-responsive properties. The primary aim of this review is to motivate researchers in both the anti-icing and gel research communities to perform a synchronized effort to rapidly advance the understanding and making of gel-based next generation anti-icing materials.
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http://dx.doi.org/10.1039/d1mh00910aDOI Listing
November 2021

Interlayer Interactions in 1D Van der Waals Moiré Superlattices.

Adv Sci (Weinh) 2021 Nov 28:e2103460. Epub 2021 Nov 28.

Department of Physics, University of California at Berkeley, Berkeley, CA, 94720, USA.

Studying two-dimensional (2D) van der Waals (vdW) moiré superlattices and their interlayer interactions have received surging attention after recent discoveries of many new phases of matter that are highly tunable. Different atomistic registry between layers forming the inner and outer nanotubes can also form one-dimensional (1D) vdW moiré superlattices. In this review, experimental observations and theoretical perspectives related to interlayer interactions in 1D vdW moiré superlattices are summarized. The discussion focuses on double-walled carbon nanotubes (DWNTs), a model 1D vdW moiré system, and the authors highlight the new optical features emerging from the non-trivial strong interlayer coupling effect and the unique physics in 1D DWNTs. Future directions and questions in probing the intriguing physical phenomena in 1D vdW moiré superlattices such as, correlated physics in different 1D moiré systems beyond DWNTs are proposed and discussed.
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http://dx.doi.org/10.1002/advs.202103460DOI Listing
November 2021

Physiological response of soybean leaves to uniconazole under waterlogging stress at R1 stage.

J Plant Physiol 2021 Nov 24;268:153579. Epub 2021 Nov 24.

College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, 524088, China; Shenzhen Research Institute, Guangdong Ocean University, Shenzhen, 518108, China. Electronic address:

Waterlogging is a major limiting factor in global crop production and seriously endangers growth and yield improvement in low-lying, rainfed regions. Soybean is an important economic crop affected by waterlogging stress. The current study investigates the effects of waterlogging stress on the leaf physiology and yield of two soybean varieties (Kenfeng 14, waterlogging-tolerant and Kenfeng 16, waterlogging-sensitive) and the mitigation effect of uniconazole (S3307) in promoting growth and productivity under waterlogging conditions. The results showed that waterlogging stress increased antioxidant enzyme activity and decreased the contents of non-enzymatic antioxidants such as AsA and GSH. Furthermore, the content of MDA and HO increased significantly, indicating oxidative stress and O production rate also improved, and the increase in the waterlogging-sensitive variety Kenfeng 16 was greater than that of the waterlogging-tolerant variety Kenfeng 14. Spraying S3307, however, increased the activities of antioxidants such as SOD, POD, CAT, and APX. GR, MDHAR, and DHAR increased the content of non-enzymatic antioxidants, effectively inhibited the increase of MDA, HO content, and O production rate, and alleviated the loss of yield factors caused by waterlogging stress. The waterlogging-tolerant variety Kenfeng 14 recovered better than the waterlogging-sensitive variety Kenfeng 16. In summary, S3307 ameliorated the effects of waterlogging stress on the physiological characteristics of soybean leaves and improved yield as a result of improved antioxidant defense mechanisms that impeded lipid peroxidation. Thus, S3307 could decelerate the damages caused by waterlogging stress to some extent.
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http://dx.doi.org/10.1016/j.jplph.2021.153579DOI Listing
November 2021

Association Between Contrast-Enhanced Ultrasound Characteristics and Molecular Subtypes of Breast Cancer.

J Ultrasound Med 2021 Nov 27. Epub 2021 Nov 27.

Department of Nuclear Medicine, Nanjing First Hospital, Nanjing Medical University, Nangjing, China.

Objective: This study aimed to investigate the correlation between contrast-enhanced ultrasound (CEUS) features and molecular subtypes of breast cancer (BC).

Methods: A total of 116 patients (116 lesions) with pathologically diagnosed BC who received conventional ultrasound and CEUS before surgery were enrolled in this study. BC molecular subtypes were identified by postoperative pathological and immunohistochemical analysis as Luminal A (LA), Luminal B (LB), HER2 (H2) over-expression, and triple-negative (TN). Qualitative and quantitative CEUS characteristics were analyzed by one-way analysis of variance (continuous variables) or Pearson's χ test or Fisher's exact probability method (categorical variables).

Results: There were significant differences in enhancement speed and enhancement degree among the four subtypes (P < .05). The area under the curve (AUC), time to peak (TTP), and peak intensity (PI) differed among the four subtypes (P < .05). The AUC of the LA subtype (305.1 ± 188.4) was significantly smaller compared with the H2 (535.7 ± 222.0, P = .007) and TN subtypes (496.6 ± 254.7, P = .019). In addition, TTP was shorter in the H2 subtype (19.8 ± 4.9) compared with the other subtypes, and was significantly shorter than in the LA subtype (26.3 ± 7.2, P = .008) and LB subtype (23.1 ± 6.7, P = .036). The PI of the LA subtype (4.7 ± 2.3) was significantly lower than that of the LB (6.6 ± 2.3, P = .027), H2 (7.4 ± 2.2, P = .005), and TN subtypes (6.9 ± 2.6, P = .014).

Conclusions: CEUS features differed significantly among different molecular subtypes of BC. The enhancement patterns and parameters may be important predictive features of different subtypes of BC.
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http://dx.doi.org/10.1002/jum.15886DOI Listing
November 2021

Impact of parental divorce versus separation due to migration on mental health and self-injury of Chinese children: a cross sectional survey.

Child Adolesc Psychiatry Ment Health 2021 Nov 27;15(1):71. Epub 2021 Nov 27.

The Institute of Social and Family Medicine, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.

Background: There has been an increasing prevalence of parental separation in China due to divorce or migration for work in recent decades. However, few studies have compared the impacts of these two types of separation on children's mental health. This study aimed to investigate how parental divorce and parental migration impact children's mental health and self-injurious thoughts and behaviors (SITB), while considering positive factors, including parent-adolescent communication and psychological resilience.

Methods: We randomly recruited participants in grades 5-8 from 18 schools in 2 counties in Anhui Province. A self-administered questionnaire was conducted to measure children's mental health, SITB, parent-adolescent communication, psychological resilience, and socio-demographic characteristics.

Results: Data from 1026 children with both parents migrating (BLBC), 1322 children with one parent migrating (SLBC), 475 children living in a divorced family (DC) and 1160 children with non-migrating parents (NLBC) were included. Regression model results showed that, compared to the other three groups (BLBC, SLBC, NLBC), DC exhibited higher internalizing problems (p < 0.05), higher externalizing problems (p < 0.01), less prosocial behaviors (p < 0.05), and higher rates of suicidal ideation (SI) (p < 0.05) and non-suicidal self-injury (NSSI) behaviors (p < 0.05) when adjusting for social-demographic variables. However, when further adjusting for parent-adolescent communication and psychological resilience, DC no longer had higher levels of internalizing problems, externalizing problems, NSSI and SI than left-behind groups (BLBC, SLBC).

Conclusions: The experience of separation from divorced parents had stronger negative effects on the mental health of children than was observed in LBC. The Chinese government should design special policy frameworks that provide support to DC.
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http://dx.doi.org/10.1186/s13034-021-00424-zDOI Listing
November 2021

Development of a Strategy for Enhancing the Biomass Growth and Lipid Accumulation of sp. UJ-3 Using Magnetic FeO Nanoparticles.

Nanomaterials (Basel) 2021 Oct 22;11(11). Epub 2021 Oct 22.

Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA.

In this study, magnetic FeO nanoparticles (NPs) were used as an effective enhancer to increase the biomass and total lipid production of sp. UJ-3. It was found that the biomass of algal cells increased significantly when they were exposed to low concentrations of FeO NPs (20 mg/L), while the best total lipid content of algal cells was achieved when they were exposed to high concentrations of FeO NPs (100 mg/L). Therefore, we established a strategy to promote the growth and lipid accumulation of microalgae by initially exposing the algal cells to low concentrations of FeO NPs and then treating them with an increased concentration of FeO NPs after 12 days of culture. For this strategy, the biomass and total lipid production of algal cells increased by 50% and 108.7%, respectively, compared to the untreated control. The increase in lipid production and change in the fatty acid composition of Chlorella cells were found to help them to cope with the increased number of reactive oxygen species produced due to oxidative stress in alga cells after the addition of FeO NPs. This study provided a highly efficient way to improve the lipid production of microalgae using nanoparticles.
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http://dx.doi.org/10.3390/nano11112802DOI Listing
October 2021

SUMO E3 Ligase SIZ1 Interacts with HDA6 and Negatively Regulates HDA6 Function during Flowering.

Cells 2021 Nov 3;10(11). Epub 2021 Nov 3.

Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.

The changes in histone acetylation mediated by histone deacetylases (HDAC) play a crucial role in plant development and response to environmental changes. Mammalian HDACs are regulated by post-translational modifications (PTM), such as phosphorylation, acetylation, ubiquitination and small ubiquitin-like modifier (SUMO) modification (SUMOylation), which affect enzymatic activity and transcriptional repression. Whether PTMs of plant HDACs alter their functions are largely unknown. In this study, we demonstrated that the SUMO E3 ligase SAP AND MIZ1 DOMAIN-CONTAINING LIGASE1 (SIZ1) interacts with HISTONE DEACETYLASE 6 (HDA6) both in vitro and in vivo. Biochemical analyses indicated that HDA6 is not modified by SUMO1. Overexpression of in background results in a decreased level of histone H3 acetylation, indicating that the activity of HDA6 is increased in plants. Chromatin immunoprecipitation (ChIP) assays showed that SIZ1 represses HDA6 binding to its target genes () and (), resulting in the upregulation of and by increasing the level of histone H3 acetylation. Together, these findings indicate that the SUMO E3 ligase SIZ1 interacts with HDA6 and negatively regulates HDA6 function.
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http://dx.doi.org/10.3390/cells10113001DOI Listing
November 2021

Impacts of p97 on Proteome Changes in Human Cells during Coronaviral Replication.

Cells 2021 Oct 29;10(11). Epub 2021 Oct 29.

Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.

Human coronavirus (HCoV) similar to other viruses rely on host cell machinery for both replication and to spread. The p97/VCP ATPase is associated with diverse pathways that may favor HCoV replication. In this study, we assessed the role of p97 and associated host responses in human lung cell line H1299 after HCoV-229E or HCoV-OC43 infection. Inhibition of p97 function by small molecule inhibitors shows antiviral activity, particularly at early stages of the virus life cycle, during virus uncoating and viral RNA replication. Importantly, p97 activity inhibition protects human cells against HCoV-induced cytopathic effects. The p97 knockdown also inhibits viral production in infected cells. Unbiased quantitative proteomics analyses reveal that HCoV-OC43 infection resulted in proteome changes enriched in cellular senescence and DNA repair during virus replication. Further analysis of protein changes between infected cells with control and p97 shRNA identifies cell cycle pathways for both HCoV-229E and HCoV-OC43 infection. Together, our data indicate a role for the essential host protein p97 in supporting HCoV replication, suggesting that p97 is a therapeutic target to treat HCoV infection.
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http://dx.doi.org/10.3390/cells10112953DOI Listing
October 2021

Preparation of Specific Nanobodies and Their Application in the Rapid Detection of Nodularin-R in Water Samples.

Foods 2021 Nov 10;10(11). Epub 2021 Nov 10.

Guangdong Provincial Key Laboratory of Food Quality and Safety, National-Local Joint Engineering Research Center for Processing and Safety Control of Livestock and Poultry Products, College of Food Science, South China Agricultural University, Guangzhou 510642, China.

Nanobodies have several advantages, including great stability, sensibility, and ease of production; therefore, they have become important tools in immunoassays for chemical contaminants. In this manuscript, nanobodies for the detection of the toxin Nodularin-r (NOD-R), a secondary metabolite of cyanobacteria that could cause a safety risk for drinks and food for its strong hepatotoxicity, were for the first time selected from an immunized Bactrian camel VHH phage display library. Then, a sensitive indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for NOD-R, based on the nanobody N56 with great thermostability and organic solvent tolerance, was established under optimized conditions. The results showed that the limit of detection for NOD-R was 0.67 µg/L, and the average spike recovery rate was between 84.0 and 118.3%. Moreover, the ic-ELISA method was validated with spiked water sample and confirmed by UPLC-MS/MS, which indicated that the ic-ELISA established in this work is a reproducible detection assay for nodularin residues in water samples.
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http://dx.doi.org/10.3390/foods10112758DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8622565PMC
November 2021

Structure Characterization of Polysaccharide from Chinese Yam ( Thunb.) and Its Growth-Promoting Effects on .

Foods 2021 Nov 4;10(11). Epub 2021 Nov 4.

College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China.

To clarify the mechanisms underlying the growth-promoting effects of yam polysaccharide on , the yam polysaccharide was extracted using a deep eutectic solvents (DESs) method and separated into four fractions by DEAE-cellulose 52. These fractions were used as the alternative carbon source to substitute lactose to compare their growth-promoting effects on . Furthermore, their molecular weight, monosaccharide and functional groups' composition, microscopic forms and other basic structure characterizations were analyzed. The results showed that all the fractions could significantly promote growth, and fractions exhibited significantly different growth-promoting effects, whose viable count increased by 6.14, 6.03, 11.48 and 11.29%, respectively, relative to those in the M17 broth medium. Structure-activity relationship analysis revealed that the high growth-promoting activity of yam polysaccharide might be more dependent on the higher molecular weight, the higher galacturonic acid content and its complex spatial configuration, and the existence of β-glycosides would make the yam polysaccharide have a better growth-promoting effect on .
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http://dx.doi.org/10.3390/foods10112698DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8624800PMC
November 2021

Preventive effect and possible mechanisms of ultrashort wave diathermy on myogenic contracture in a rabbit model.

Sci Prog 2021 Oct;104(4):368504211054992

Department of Rehabilitation Medicine, 533251The Second Hospital of Anhui Medical University, Hefei, Anhui, China.

The purpose of this study was to determine the preventive effect of ultrashort wave diathermy on immobilization-induced myogenic contracture and to explore its underlying mechanisms. Forty-two rabbits were randomly assigned into control (Group C), immobilization (Group I, which was further divided into one week, Group I-1; two weeks, Group I-2; and four weeks, Group I-4, subgroups by the length of immobilization) and ultrashort wave prevention (Group U, which was further divided into one week, Group U-1; two weeks, Group U-2; and four weeks, Group U-4, by time of treatment) groups. Intervention effects were assessed by evaluating rectus femoris cross-sectional area (CSA), knee range of motion, and the protein levels for myogenic differentiation (MyoD) and muscle atrophy F-box (MAFbx-1) in the rectus femoris. Compared with those of Group C, in Groups I and U, total contracture, myogenic contracture, MyoD and MAFbx-1 levels were significantly elevated, and CSA was significantly smaller ( < 0.05). Compared with those of Group I at each time point, MyoD levels were significantly elevated, MAFbx-1 levels were significantly lower, CSA was significantly larger, and myogenic contracture was significantly alleviated in Group U ( < 0.05). In the early stages of contracture, ultrashort wave diathermy reduces muscle atrophy and delays the process of myogenic contracture during joint immobilization; the mechanism of this may be explained as increased expression of MyoD triggered by suppression of the MAFbx-1-mediated ubiquitin-proteasome pathway.
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http://dx.doi.org/10.1177/00368504211054992DOI Listing
October 2021

Conductive bimetal organic framework nanorods decorated with highly dispersed Co3O4 nanoparticles as bi-functional electrocatalyst.

Nanotechnology 2021 Nov 25. Epub 2021 Nov 25.

Beijing University of Chemical Technology, Beijing University of Chemical Technology, 15 Beisanhuan East Road Beijing, CN 100029, Beijing, Beijing, CN 100029, CHINA.

The poor electronic conductivity and low intrinsic electrocatalytic activity of metal organic frameworks (MOFs) greatly limit their direct application in electrocatalytic reactions. Herein, we report a conductive two-dimensional π-d conjugated Ni and Co bimetal organic framework (MOF)--NiCo-(2,3,6,7,10,11-hexaiminotriphenylene) (NiCo-HITP) nanorods decorated with highly dispersed Co3O4 nanoparticles (NPs) as a promising bi-functional electrocatalyst towards oxygen reduction reaction (ORR) and oxygen evolution reaction (OER) through an effective and facile strategy by modifying the rod-shaped Ni3(HITP)2 crystals using cobalt ions. The triggered electrocatalytic activity of the resulting MOF-based materials was achieved by increasing the electrical conductivity (7.23 S cm-1) originated from Ni3(HITP)2 substrate and also by creating the cooperative catalysis sites of Co-Nx and Co3O4 NPs. Optimized syntheses show a promising ORR activity with a high half-wave potential (0.77 V) and also a significantly improved OER activity compared with pure Ni3(HITP)2 in alkaline electrolyte. Furthermore, a rechargeable Zn-air battery using the as-prepared material as air-cathode also shows a high power density (143.1 mW cm-2) -even comparable to a commercial Pt/C-RuO2-based battery. This methodology offers a new prospect in the design and synthesis of non-carbonized MOF bi-functional electrocatalysts for efficient catalysis towards ORR and OER.
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http://dx.doi.org/10.1088/1361-6528/ac3d66DOI Listing
November 2021

Rice Brittle Culm19 Encoding Cellulose Synthase Subunit CESA4 Causes Dominant Brittle Phenotype But has No Distinct Influence on Growth and Grain Yield.

Rice (N Y) 2021 Nov 25;14(1):95. Epub 2021 Nov 25.

State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, China.

Background: Mechanical strength is a crucial agronomic trait in rice (Oryza sativa), and brittle mutants are thought suitable materials to investigate the mechanism of cell wall formation. So far, almost all brittle mutants are recessive, and most of them are defected in multiple morphologies and/or grain yield, limiting their application in hybrid breeding and in rice straw recycling.

Results: We identified a semi-dominant brittle mutant Brittle culm19 (Bc19) isolated from the japonica variety Nipponbare through chemical mutagenesis. The mutant showed the same apparent morphologies and grain yield to the wild type plant except for its weak mechanical strength. Its development of secondary cell wall in sclerenchyma cells was affected, along with reduced contents of cellulose, hemicellulose, lignin and sugars in culms and leaves. Positional cloning suggested that the Bc19 gene was allelic to OsCESA4, encoding one of the cellulose synthase A (CESA) catalytic subunits. In this mutant, a C-to-T substitution occurred in the coding sequence of BC19, causing the P507S missense mutation in its encoded product, which was located in the second cytoplasmic region of the OsCESA4 protein. Furthermore, introducing mutant gene Bc19 into the wild-type plant resulted in brittle plants, confirming that the P507S point mutation in OsCESA4 protein was responsible for the semi-dominant brittle phenotype of Bc19 mutant. Reverse correlation was revealed between cellulose contents and expression levels of mutant gene Bc19 among the homozygous mutant, the hybrid F plant, and the Bc19 overexpression transgenic plants, implying that gene Bc19 might affect cellulose synthesis in a dosage-dependent manner.

Conclusions: Bc19, a semi-dominant brittle mutant allele of gene OsCESA4, was identified using map-based cloning approach. The mutated protein of Bc19 possessing the P507S missense mutation behaved in a dosage-dependent semi-dominant manner. Unique brittle effect on phenotype and semi-dominant genetic quality of gene Bc19 indicated its potential application in grain-straw dual-purpose hybrid rice breeding.
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http://dx.doi.org/10.1186/s12284-021-00536-2DOI Listing
November 2021

Risk Factor and Surgical Outcome of Petersen's Hernia After Gastrectomy in Gastric Cancer.

Front Oncol 2021 8;11:765509. Epub 2021 Nov 8.

Department of Gastrointestinal Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, China.

Background: Petersen's hernia is a life-threatening complication after gastrectomy. This study is dedicated to identify risk factors for Petersen's hernia and compare clinical outcomes between patients receiving early or delayed surgical interventions.

Methods: Data from all patients who received gastrectomy due to gastric cancer were collected. Clinical characteristics were compared between Petersen and non-Petersen groups, bowel necrosis and non-necrotic groups. Propensity score matching (PSM) was conducted to generate two comparative groups. Univariate analysis and multivariate logistic regression were performed for risk factor evaluation.

Results: A total of 24 cases of Petersen's hernia were identified from 1,481 cases of gastrectomy. PSM demonstrated that lower body mass index [BMI; odds ratio (OR) = 0.2, p < 0.01] and distal gastrectomy (OR = 6.2, p = 0.011) were risk factors for Petersen's hernia. Longer time interval from emergence visit to laparotomy (p = 0.042) and elevated preoperative procalcitonin (p = 0.033) and C-reactive protein (CRP; p = 0.012) were associated with higher risk of bowel necrosis in Petersen's hernia. Early surgical intervention resulted in less bowel necrosis rate (p = 0.012) and shorter length of necrotic bowel (p = 0.0041).

Conclusions: Low BMI and distal gastrectomy are independent risk factor for Petersen's hernia after gastrectomy. Curtailing observing time and executing prompt surgery are associated with bowel viability and better outcome in patients with Petersen's hernia.
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http://dx.doi.org/10.3389/fonc.2021.765509DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8606515PMC
November 2021

Sult2b1 deficiency exacerbates ischemic stroke by promoting pro-inflammatory macrophage polarization in mice.

Theranostics 2021 1;11(20):10074-10090. Epub 2021 Nov 1.

Shanghai Institute of Immunology, Translational Medicine Center, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Stroke is a leading causes of human death worldwide. Ischemic damage induces the sterile neuroinflammation, which directly determines the recovery of patients. Lipids, a major component of the brain, significantly altered after stroke. Cholesterol sulfate, a naturally occurring analog of cholesterol, can directly regulate immune cell activation, indicating the possible involvement of cholesterol metabolites in neuroinflammation. Sulfotransferase family 2b member 1 (Sult2b1) is the key enzyme that catalyzes the synthesis of cholesterol sulfate. This study aimed to investigate the function of Sult2b1 and cholesterol sulfate in the neuroinflammation after ischemic stroke. : and wild-type mice were subjected to transient middle cerebral artery occlusion. Our data showed that mice had larger infarction and worse neurological scores. To determine whether immune cells were involved in the worsening stroke outcome in mice, bone marrow transplantation, immune cell depletion, and adoptive monocyte transfer were performed. Combined with CyTOF and immunofluorescence techniques, we demonstrated that after stroke, the peripheral monocyte-derived macrophages were the dominant cell type promoting the pro-inflammatory status in mice. Using primary bone marrow-derived macrophages, we showed that cholesterol sulfate could attenuate the pro-inflammatory polarization of macrophages under both normal and oxygen-glucose deprivation conditions by regulating the levels of nicotinamide adenine dinucleotide phosphate (NADPH), reactive oxygen species (ROS), and activating the AMP-activated protein kinase (AMPK) - cAMP responsive element-binding protein (CREB) signaling pathway. promoted the polarization of macrophages into pro-inflammatory status. This trend could be attenuated by adding cholesterol sulfate, which promotes the polarization of macrophages into anti-inflammatory status by metabolic regulation. In this study, we established an inflammation-metabolism axis during the macrophage polarization after ischemic stroke.
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http://dx.doi.org/10.7150/thno.61646DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8581421PMC
November 2021

SARS-CoV-2 receptor binding domain radio-probe: a non-invasive approach for angiotensin-converting enzyme 2 mapping in mice.

Acta Pharmacol Sin 2021 Nov 23. Epub 2021 Nov 23.

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), NMPA Key Laboratory for Research and Evaluation of Radiopharmaceuticals (National Medical Products Administration), Department of Nuclear Medicine, Peking University Cancer Hospital & Institute, Beijing, 100142, China.

The spike protein of SARS-CoV-2 interacts with angiotensin-converting enzyme 2 (ACE2) of human respiratory epithelial cells, which leads to infection. Furthermore, low-dose radiation has been found to reduce inflammation and aid the curing of COVID-19. The receptor binding domain (RBD), a recombinant spike protein with a His tag at the C-terminus, binds to ACE2 in human body. We thus constructed a radioiodinated RBD as a molecule-targeted probe to non-invasively explore ACE2 expression in vivo, and to investigate radiotherapy pathway for inhibiting ACE2. RBD was labeled with [I]NaI using an N-bromosuccinimide (NBS)-mediated method, and I-RBD was obtained after purification with a specific activity of 28.9 GBq/nmol. Its radiochemical purity was (RCP) over 90% in saline for 5 days. The dissociation constant of I-RBD binding to hACE2 was 75.7 nM. The uptake of I-RBD by HeLa cells at 2 h was 2.96% ± 0.35%, which could be substantially blocked by an excessive amount of RBD, and drop to 1.71% ± 0.23%. In BALB/c mice, the biodistribution of I-RBD after intravenous injection showed a moderate metabolism rate, and its 24 h-post injection (p.i.) organ distribution was similar to the expression profile in body. Micro-PET imaging of mice after intrapulmonary injection showed high uptake of lung at 1, 4, 24 h p.i.. In conclusion, the experimental results demonstrate the potential of I-RBD as a novel targeted molecular probe for COVID-19. This probe may be used for non-invasive ACE2 mapping in mammals.
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http://dx.doi.org/10.1038/s41401-021-00809-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8609177PMC
November 2021

Unusual Site-Selective Doping in Layered Cathode Strengthens Electrostatic Cohesion of Alkali-Metal Layer for Practicable Sodium-ion Full Cell.

Adv Mater 2021 Nov 22:e2103210. Epub 2021 Nov 22.

Hefei National Laboratory for Physical Sciences at the Microscale, CAS Key Laboratory of Materials for Energy Conversion, Department of Materials Science and Engineering, University of Science and Technology of China, Hefei, Anhui, 230026, China.

P2-type Na Ni Mn O is a dominant cathode material for sodium-ion batteries (SIBs) due to its high theoretical capacity and energy density. However, charging P2-type Na Ni Mn O to voltages higher than 4.2 V versus Na /Na could induce detrimental structural transformation and severe capacity fading. Herein, we achieve stable cycling and moisture resistancy of Na Ni Mn O at 4.35 V (versus Na /Na) through dual-site doping with Cu ion at transition metal site (2a) and unusual Zn ion at Na site (2d), for the first time. The Cu ion doping in 2a site stabilizes the metal layer, while more importantly, the unusual alkali-metal site doping by Zn ion serves as O -Zn -O "pillar" for enhancing electrostatic cohesion between two adjacent transition metal layers, preventing the crack of active material along a-b plane and restraining the generation of O2 phase upon deep desodiation. This unique dual-site doped [Na Zn ]Ni Cu Mn O cathode exhibits a prominent cyclability with 80.6% capacity retention over 2000 cycles at an ultrahigh rate of 10C, demonstrating its great potential for practical applications. Impressively, the full cell devices with [Na Zn ]Ni Cu Mn O and commercial hard carbon as cathode and anode, respectively, can deliver a high energy density of 217.9 Wh kg and excellent cycle life over 1000 cycles. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/adma.202103210DOI Listing
November 2021

Contralateral PosteriorPutaminal 18F-Fluorodopa Uptake in Mild Stage Parkinson's Disease: a PET/CT Study.

Curr Neurovasc Res 2021 Nov 21. Epub 2021 Nov 21.

Department of Nuclear Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing. China.

Objective: Previous studies revealed that 18F-FDOPA uptake was significantly decreased in the subregions of striatum contralateral to the side with predominant symptoms and was helpful for improving the early diagnostic accuracy of PD. However, in these studies, more than half of the PD patients already have bilateral motor symptoms (mH&Y stage≥2). This study was aimed to extend previous findings to a milder disease stage.

Methods: Sixteen PD patients with only mild and unilateral motor symptoms (mH&Y stage=1 and disease duration≤2 years) and 22 healthy controls were involved. Striatal 18F-FDOPA uptake was analyzed using a ratio approach.

Results: The SORs in the subregions of the contralateral striatum, including caudate, anterior putamen and posterior putamen were significantly decreased in the mild stage PD patients. The SOR for the contralateral posterior putamen had the largest area under the receiver operating characteristic curve (0.963) and separated mild stage PD patients from healthy controls with a sensitivity of 93.75% and a specificity of 95.45% when the cut-off value of <2.160 was selected.

Conclusion: These data indicate that contralateral posterior putaminal 18F-FDOPA uptake may represent a potential marker for early diagnosis of PD, especially in patients with only mild and unilateral motor symptoms.
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http://dx.doi.org/10.2174/1567202618666211122091108DOI Listing
November 2021

Attosecond photoemission delay in the inhomogeneous field.

Opt Express 2021 Nov;29(24):39729-39737

We investigate the photoemission process of the hydrogen atom in a spatial-dependent infrared (IR) field. The results show that the inhomogeneous field induces an additional contribution to the photoemission time delay, which results in the increase (decrease) of the photoemission time delay due to the enhancement (decay) of the IR field intensity in space when compared to the case in the homogeneous field. Based on the photoemission time delay in the inhomogeneous field, we demonstrate a method to extract the inhomogeneous parameter that is vital for characterizing the spatial distribution of IR field. The proposed method might pave an accessible route toward describing the plasmon-enhanced fields in the vicinity of a nanostructure.
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http://dx.doi.org/10.1364/OE.441986DOI Listing
November 2021

A novel asymmetric CDI device for targeted removal of cation in water desalination.

Environ Technol 2021 Nov 22:1-34. Epub 2021 Nov 22.

Hebei Key Lab of Power Plant Flue Gas Multi-Pollutants Control, Department of Environmental Science and Engineering, North China Electric Power University, Baoding 071003, PR China.

A novel asymmetric CDI device was home-made for the removal of cations from aqueous solution through the structural innovation in this experiment. The adsorbent materials were characterized by SEM and BET as well as performing CV curve tests. The results showed that the materials exhibited excellent performance(the Specific surface area is 321.14 m²/g) and the maximum specific capacitance of the adsorbent material can reach 60F/g at the 5mV/s. The optimal operating conditions of the asymmetric CDI device were obtained using an orthogonal test method analysis and response surface methodology (RSM): the best desalination efficiency of CDI was obtained at concentration is 455.82 mg/L, potential is 1.18 V and flow rate is 46.54 mL/min. After the analysis of first-order kinetic model,it can be obtained that the fastest adsorption efficiency for Ca, followed by Mg, and the slowest for Na, and the CDI device had better adsorption effect on divalent cations. Simulation by Comsol software showed that the adsorption efficiency was better at high flow rate. In addition, the CDI device has good stability, and the mechanism of targeted cation removal and mass transfer process of the CDI were discussed in detail.
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http://dx.doi.org/10.1080/09593330.2021.2010129DOI Listing
November 2021

Achieving a Stable Solid Electrolyte Interphase and Enhanced Thermal Stability by a Dual-Functional Electrolyte Additive toward a High-Loading LiNiMnCoO /Lithium Pouch Battery.

ACS Appl Mater Interfaces 2021 Nov 22. Epub 2021 Nov 22.

State Key Lab of Physical Chemistry of Solid Surfaces, Collaborative Innovation Centre of Chemistry for Energy Materials, State-Province Joint Engineering Laboratory of Power Source Technology for New Energy Vehicle, Engineering Research Center of Electrochemical Technology, Ministry of Education, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, P.R. China.

Li metal batteries with high-capacity cathodes emerge as promising candidates for next-generation battery technologies. However, the poor reversibility of the Li deposition/stripping process severely reduces its lifespan, and safety also remains a major issue for the Li metal anodes. Herein, we propose (ethoxy)-penta-fluoro-cyclo-triphosphazene (DFA) as a dual-functional electrolyte additive to solve the engineering problem of balancing the cycle life and thermal stability of Li metal batteries. The NCM811/lithium metal pouch batteries (2900 mA h) are assembled using the commercial high areal capacity cathode (3.5 mA h cm). Compared with the NCM811/Li batteries without DFA, the heat generation and heat generation power of lithium metal batteries with DFA are significantly reduced by half during charging. Moreover, the NCM811/Li pouch batteries with DFA show excellent stability in both hot-oven and adiabatic rate calorimeter experiments. Furthermore, a nonlinear phase field simulation is carried out for mechanism investigation, which confirms that the stable solid electrolyte interphase formed by DFA will improve the cycle life of the NCM811/Li pouch. The DFA is verified to be an effective additive to improve the cycle stability and safety simultaneously, providing new opportunities for developing high energy density Li metal batteries.
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http://dx.doi.org/10.1021/acsami.1c17209DOI Listing
November 2021

Development of point diffraction interferometer by a dimension-reduction-based phase-shifting algorithm.

Appl Opt 2021 Oct;60(30):9440-9446

To avoid exhaustive calibration of the shifter device in point diffraction interferometers, we present a dimension-reduction-based method to reconstruct the phase map from more phase-shifting fringe patterns with three or more frames. The proposed method assumes that the intensity space can be described adequately by the sine and cosine of multiple phase shifts introduced, which are the basis of the intensity space. Then, low-dimensional approximations of high-dimensional intensity spaces are determined by the newly developed reduced basis decomposition technique. Finally, the phase is reconstructed using the low-dimensional surrogates of the intensity spaces without the knowledge of accurate phase steps. Numerical and experimental studies demonstrated that the proposed method outperforms the existing popular phase reconstruction techniques in terms of accuracy and efficiency. Moreover, the performance of the proposed method is not limited by variations in the background and modulation, unlike the existing phase-shifting-algorithm-based approaches.
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http://dx.doi.org/10.1364/AO.439512DOI Listing
October 2021

Safety and effectiveness of an iris hook assisted phacoemulsification in vitrectomized eyes.

Int J Ophthalmol 2021 18;14(11):1735-1740. Epub 2021 Nov 18.

Experimental Teaching Center for Clinical Skills, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China.

Aim: To introduce a simple iris hook assisted phacoemulsification (PE) procedure and evaluate the safety and efficacy of it in completely vitrectomized eyes.

Methods: A single centre study which included 65 previously completely vitrectomized eyes of 62 patients who underwent cataract surgery. Patients were randomly divided into 3 groups. Patients received PE, and intraocular lens (IOL) implantation with the assistance of iris hook (Synergeties™) as group A (25 eyes); patients who received PE assisted with a 25G pars plana irrigation as group B (20 eyes), and patients who received PE performed without the help of any instrument as group C (20 eyes). Main outcome measures were surgery duration, Ultrasound (U/S) total time, endothelial cell density (ECD), cumulative dissipated energy (CDE) and complications of the procedures.

Results: With the help of iris hook, the patients in group A had the lowest ECD loss rate (0.07±0.03, 0.09±0.03, and 0.10±0.03, <0.05), shortest CDE (12.2±4.1, 15.8±6.0, and 16.0±6.0, <0.05) and U/S total time (36.6±13.0s, 46.3±16.4s, and 47.6±16.1s, <0.05), and minimal incidence of complications. The longest surgery duration was in group B (19.4±1.6min) and maximum complications rate in group C (20% miosis, 10% posterior capsular tears, 5% zonular dialysis, 5% cystoid macular edema). While best-corrected visual acuity (BCVA), intraocular pressure (IOP) and ECD did not show a significant difference among the three groups.

Conclusion: Without prolonged surgery duration, the iris hook assistant method can minimize heat generation during surgery and incidence of complications, which transfer the challenged PE in vitrectomized eyes into a regular surgery. It does not need any change in the hydrodynamic parameters and in the bag PE technique, easy to operate even for junior surgeons.
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http://dx.doi.org/10.18240/ijo.2021.11.13DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8569570PMC
November 2021

Causes and Preventive Measures of Sports Injuries in Physical Fitness Tests in Colleges and Universities Based on Biological Characteristics.

Authors:
Feng Wang

J Healthc Eng 2021 11;2021:2280205. Epub 2021 Nov 11.

School of Physical Education, Wuhan Business University, Wuhan 430056, Hubei, China.

Biological characteristics refer to the morphological and physiological characteristics exhibited by individual organisms. The indicators of individual biological characteristics mainly include physical indicators such as height, weight, chest circumference, and health-related physical indicators such as blood pressure, grip strength, and vital capacity. This article mainly introduces the cause analysis and preventive measures of a physical fitness test in colleges and universities based on biological characteristics and intends to provide ideas and directions for the cause analysis and preventive measures of a physical fitness test in colleges and universities. This paper proposes a research method for the cause analysis and preventive measures of sports injuries in college physical fitness tests based on biological characteristics. Research experiments on the cause analysis and preventive measures of sports injuries in the physical fitness test of colleges and universities. The experimental results in this paper show that the most frequent cause of sports injuries among boys is lack of self-protection awareness, with a probability of 24.24%; among girls, the most frequent cause of sports injuries is insufficient flexibility, with a probability of 22.86%.
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http://dx.doi.org/10.1155/2021/2280205DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8601814PMC
November 2021

HLA-DR Expression Level in CD8 T Cells Correlates With the Severity of Children With Acute Infectious Mononucleosis.

Front Immunol 2021 3;12:753290. Epub 2021 Nov 3.

Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: This study aimed to assess the host immune signatures associated with EBV infection and its clinical value in indicating the severity of children with acute infectious mononucleosis (IM).

Methods: Twenty-eight pediatric patients with IM aged 3-8 years were enrolled. The immune phenotypes and cytokine secretion capability of T cells were detected.

Results: The percentages and absolute numbers of CD3 and CD8 T cells were significantly increased in IM patients compared with HCs. The percentages of Naïve CD4 and CD8 T cells were decreased but with increased percentages of memory CD4 and CD8 T subsets. Our results showed the upregulation of active marker HLA-DR, TCR-αβ, and inhibitory receptors PD-1, TIGIT in CD8 T cells from IM patients, which suggested that effective cytotoxic T cells were highly against EBV infection. However, EBV exposure impaired the cytokine (IFN-γ, IL-2, and TNF-α) secretion capability of CD4 and CD8 T cells after stimulation with PMA/ionomycin . Multivariate analysis revealed that the percentage of HLA-DR CD8 T cells was an independent prognostic marker for IM. The percentage of HLA-DR CD8 T cells was significantly correlated with high viral load and abnormal liver function results.

Conclusion: Robust expansion and upregulation of HLA-DR in CD8 T cells, accompanied with impaired cytokine secretion, were typical characteristics of children with acute IM. The percentage of HLA-DR CD8 T cells might be used as a prominent marker not only for the early diagnosis but also for indicating the severity of IM.
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http://dx.doi.org/10.3389/fimmu.2021.753290DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8596082PMC
November 2021

Application value and feasibility analysis of cervical cerebrovascular ultrasonography in the diagnosis of coronary atherosclerotic cerebral infarction.

Minerva Surg 2021 Nov 17. Epub 2021 Nov 17.

Department of Ultrasonography, Affiliated Hospital of Chengde Medical University, Chengde, China.

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http://dx.doi.org/10.23736/S2724-5691.21.09227-3DOI Listing
November 2021

Identification of a novel iron zinc finger protein 36 (ZFP36) for predicting the overall survival of osteosarcoma based on the Gene Expression Omnibus (GEO) database.

Ann Transl Med 2021 Oct;9(20):1552

Department of Spinal Surgery, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, China.

Background: The purpose of this study is to explore the relationship between the ferroptosis-related gene zinc finger protein 36 (ZFP36) and the prognosis of osteosarcoma patients after surgery.

Methods: Differential expression genes (DEGs) between osteosarcoma and normal tissues were screened using osteosarcoma chip data in GEO database. Based on the median expression quantity, ferroptosis DEGs were divided into high and low expression groups. Combined with its corresponding clinical survival data, the survival analysis of ferroptosis DEGs was carried out using the Survival package, and ferroptosis-related genes related to prognosis were identified. Next, the clinical data of 60 osteosarcoma patients treated in Jiangyin Hospital Affiliated to Nanjing University of Chinese Medicine, Zhongda Hospital and Nanjing Drum Tower Hospital from January 2011 to January 2016 were retrospectively analyzed. Immunohistochemistry and reverse transcription quantitative polymerase chain reaction (qRT-PCR) were used to detect gene expression in osteosarcoma. The Kaplan-Meier method and log rank test were used for univariate survival analysis, the Cox regression method was used for multivariate analysis, and the nomogram was constructed for internal verification on this basis.

Results: Immunohistochemical and reverse transcription quantitative PCR results showed that the expression of ZFP36 was mainly higher in cancer-adjacent tissues than in tumor tissues. There were significant differences in age, tumor location, Enneking stage, and tumor specific growth factor (TSGF) between the high and low expression groups of ZFP36 (P<0.05). The final study included 60 patients, of whom 23 patients died (mortality rate: 38.33%), and 37 patients survived (survival rate: 61.67%), with a median progression-free survival (PFS) of 32.5 months and a median overall survival (OS) of 77 months. The Cox multivariate analysis showed that distant metastasis and ZFP36 were independent risk factors affecting tumor progression (P=0.021 and P=0.006, respectively). Elevated ZFP36 can significantly prolong the OS and PFS of osteosarcoma patients. In internal verification, the Concordance index (C-index) of the nomogram was 0.7211 [95% confidence interval (CI): 0.6308-0.8115], and the prediction model had certain accuracy.

Conclusions: Elevated ZFP36 can significantly prolong the OS and PFS in osteosarcoma patients. At the same time, ZFP36 could be used as a new predictive biomarker and novel therapeutic target for osteosarcoma patients.
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http://dx.doi.org/10.21037/atm-21-5086DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8576698PMC
October 2021

Measuring phonon dispersion at an interface.

Nature 2021 Nov 17;599(7885):399-403. Epub 2021 Nov 17.

Electron Microscopy Laboratory, School of Physics, Peking University, Beijing, China.

The breakdown of translational symmetry at heterointerfaces leads to the emergence of new phonon modes localized at the interface. These modes have an essential role in thermal and electrical transport properties in devices, especially in miniature ones wherein the interface may dominate the entire response of the device. Although related theoretical work began decades ago, experimental research is totally absent owing to challenges in achieving the combined spatial, momentum and spectral resolutions required to probe localized modes. Here, using the four-dimensional electron energy-loss spectroscopy technique, we directly measure both the local vibrational spectra and the interface phonon dispersion relation for an epitaxial cubic boron nitride/diamond heterointerface. In addition to bulk phonon modes, we observe modes localized at the interface and modes isolated from the interface. These features appear only within approximately one nanometre around the interface. The localized modes observed here are predicted to substantially affect the interface thermal conductance and electron mobility. Our findings provide insights into lattice dynamics at heterointerfaces, and the demonstrated experimental technique should be useful in thermal management, electrical engineering and topological phononics.
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http://dx.doi.org/10.1038/s41586-021-03971-9DOI Listing
November 2021

Renal tubular cell necroptosis: A novel mechanism of kidney damage in trichloroethylene hypersensitivity syndrome mice.

J Immunotoxicol 2021 12;18(1):173-182

Department of Dermatology, The First Affiliated Hospital of Anhui Medical University, Hefei, PR China.

Trichloroethylene (TCE) hypersensitivity syndrome (THS), called occupational medicamentosa-like dermatitis due to TCE (OMDT) in China, is a fatal occupational disorder caused by TCE exposure. Visceral damage, including kidney injury, is one of the major complications. Necroptosis is a regulated cell death form linked to local inflammatory response. This study aimed to investigate whether renal cell necroptosis was involved in TCE-induced kidney injury. A Balb/c mouse model of TCE sensitization was utilized to study mechanisms of modulation of TCE-induced renal necroptosis. Renal histology (using light and transmission electron microscopy) and renal tubular impairment indexes, including α1-microglobulin (α1-MG), and β2-microglobulin (β2-MG), were evaluated. In addition, tissue expression of necroptosis-related proteins, including tumor necrosis factor (TNF)-α, TNF receptor 1 (TNFR1), receptor-interacting protein kinase 3 (RIPK3), p-RIK3, mixed lineage kinase domain-like protein (MLKL), and p-MLKL, were also evaluated. The study here confirmed TCE sensitization caused damage to renal tubules and renal tubular epithelial cell (RTEC) necroptosis. In mice treated with R7050 (a specific TNFα antagonist), it was also seen that inhibition of TNFα expression could effectively inhibit RTEC necroptosis and improve renal function in the TCE-sensitized mice. Taken together, these results help to define a novel mechanism by which RTEC necroptosis plays a key role in TCE-induced kidney damage.
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http://dx.doi.org/10.1080/1547691X.2021.2003486DOI Listing
December 2021
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