Publications by authors named "Wanaporn Tapingkae"

9 Publications

  • Page 1 of 1

Impacts of pineapple peel powder on growth performance, innate immunity, disease resistance, and relative immune gene expression of Nile tilapia, Oreochromis niloticus.

Fish Shellfish Immunol 2021 Apr 9. Epub 2021 Apr 9.

Fish Nutrition Research Laboratory, Animal Production Department, Faculty of Agriculture, Cairo University, Cairo, Egypt.

An 8-week growth trial was conducted to examine the efficacy of pineapple peel powder (PAPP) on growth rate and immunity of Nile tilapia, O. niloticus. Three hundred Nile tilapia (20.91 ± 0.11 g) were fed five diets containing different levels of PAPP at 0, 10, 20, 30 and 40 g kg PAPP, respectively. After four and eight weeks of the feeding trial, growth rates, and immune responses were tested. A challenge test using Streptococcus agalactiae and relative immune gene expression were performed after eight weeks of PAPP feeding. It was found that skin mucus and serum lysozyme, skin mucus and serum peroxidase, alternative complement, phagocytosis, and respiratory burst activities were significantly increased with the addition of PAPP. The maximum (P≤0.05) innate immune values were noted in fish fed 10 g kg PAPP. Similarly, the up-regulation of IL1, IL8, and LBP gene expressions were also detected in fish fed PAPP diets, with the maximum value was found in 10 g kg PAPP fed fish. The relative percentage of survival (RPS) of Oreochromis niloticus after the challenge test were (56.00%, 72.00%, 60.00%, and 44.00%) for the 5, 10, 20 and 40 g kg PAPP diets, respectively. Fish fed the 10 g kg PAPP supplemented diet achieved the highest (P<0.05) survival rate against S. agalactiae. Growth and feed efficiency were outstandingly (P<0.05) enhanced in the PAPP groups. In conclusion, PAPP can be potentially used as a feed additive in Nile tilapia culture under Biofloc system.
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http://dx.doi.org/10.1016/j.fsi.2021.04.002DOI Listing
April 2021

Investigation on the Double CutOff Phenomenon Observed in Protocatechuic Acid and Its Alkyl Esters under Various CAT-Based Assays.

J Agric Food Chem 2020 Sep 24;68(35):9568-9575. Epub 2020 Aug 24.

Food Biotechnology Research Team,f Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Phaholyothin Rd., Klong Luang, Pathumthani 12120, Thailand.

A strange cutoff phenomenon of a series of protocatechuic acid alkyl esters had been noticed using the conjugated autoxidizable triene (CAT) assay. Two parabolic shapes of antioxidant activities of protocatechuic acid alkyl esters described as ″the double cutoff effect″ have been speculated as a result of an oxidative driving force generated in the aqueous phase. The aim of this research was to investigate the double cutoff effect using various types of oxidation driving forces in different CAT-based assays. To further explain the phenomenon, the natural oxidation of conjugated autoxidizable triene (NatCAT) assay has been developed for the first time by relying solely on only the lipid autoxidation of tung oil-in-water (O/W) emulsions. In conclusion, NatCAT exhibited different antioxidant and oxidation patterns from both CAT and apolar radical-initiated CAT assays, and only one cutoff point was obtained. This discovery would lead to a greater understanding of the complexity of antioxidant/lipid oxidation dynamics in O/W emulsion systems.
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http://dx.doi.org/10.1021/acs.jafc.0c03825DOI Listing
September 2020

Modulation of mucosal parameters, innate immunity, growth and resistance against Streptococcus agalactiae by enrichment of Nile tilapia (Oreochromis niloticus) diet with Leucas aspera.

Fish Shellfish Immunol 2020 Feb 14;97:165-172. Epub 2019 Dec 14.

School of Ocean Science and Technology, Kerala University of Fisheries and Ocean Studies Panangad, Kochi, 682 506, Kerala, India. Electronic address:

The present study aimed at evaluating the possible effects of Leucas aspera as immunostimulant on mucosal and serum immunity, as well as on growth and resistance against Streptococcus agalactiae infection in Nile tilapia (Oreochromis niloticus) fingerlings. In a 45 days trial, fish were fed experimental diets containing L. aspera 0 g kg (Diet 1- control), 1 g kg (Diet 2), 2 g kg(Diet 3), 4 g kg (Diet 4) and 8 g kg (Diet 5). The results revealed a significant increase in the specific growth rate (SGR), weight gain (WG), and final weight (FW) in fish fed diet 3 (2 g kg) of L. aspera compared to the control and other supplemented groups (P < 0.05). Also, feeding on diet 3 (2 g kg) of L. aspera enriched diet significantly (P < 0.05) increased lysozyme activities in the serum and mucus, serum peroxidase and phagocytosis activity. However, significant (P < 0.05) increase in mucus peroxidase activity was reported in fish fed diet 4 (4 g kg) and diet 5 (8 g kg) of L. aspera, whereas significantly higher (P < 0.05) alternative complement activity was reported in fish fed diet 2 (1 g kg) of L. aspera. At the end of the experiment, nine fish per replication were selected for a challenge test against S. agalactiae. The dietary supplementation of L. aspera significantly reduced the mortality rate and increased the resistance of Nile tilapia following by challenge with S. agalactiae. The highest post challenge survival of 100% was observed in tilapia fed diet 5 (8 g kg) following by 92.6% of RPS in fish fed diet 4 (4 g kg) and 88.9% in diet 3 (2 g kg), 77.8% in diet 2 (1 g kg) and 74.1% in diet 1(0 g kg).
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http://dx.doi.org/10.1016/j.fsi.2019.12.043DOI Listing
February 2020

Boosted Growth Performance, Mucosal and Serum Immunity, and Disease Resistance Nile Tilapia (Oreochromis niloticus) Fingerlings Using Corncob-Derived Xylooligosaccharide and Lactobacillus plantarum CR1T5.

Probiotics Antimicrob Proteins 2020 06;12(2):400-411

Fish Innate Immune System Group, Department of Cell Biology & Histology, Faculty of Biology, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, Murcia, Spain.

The present work, herein, studied the effects of corncob-derived xylooligosaccharides (CDXOS) and Lactobacillus plantarum CR1T5 (LP) integrated into fish diets (diet 1 (0-control), diet 2 (10 g kg CDXOS), diet 3 (10 CFU gL. plantarum CR1T5), diet 4 (10 g kg CDXOS +10 CFU gL. plantarum CR1T5)) on growth performance, innate immune parameters, and disease resistance of Nile tilapia (Oreochromis niloticus). Fingerlings, with average mean weight of 4.97 ± 0.04, were randomly distributed into 16 glass tanks (20 fish per tank) for 12 weeks. Growth performance, skin mucus, and serum immune parameters were evaluated at the conclusion of the experiment. Eight randomly selected fish were used for challenge test against Streptococcus agalactiae. The results indicated that fish fed CDXOS and LP had significantly improved final weight (FW), weight gain (WG), specific growth rate (SGR), and feed conversion ratio (FCR). However, no significant difference in survival rate was observed between specimens fed the supplemented diets and the control. Regarding skin mucus, the dietary inclusion of CDXOS and LP significantly increased lysozyme and peroxidase activities compared with the control (P < 0.05). Similarly, significant increases in serum lysozyme, peroxidase, alternative complement, phagocytosis, and respiratory burst activities were observed in the fish fed the supplemented diets. However, no significant differences were found in these parameters between fish fed CDXOS and LP diets. For the challenge test, diet 4 produced a higher relative percentage of survival (RPS) and resistance to S. agalactiae than fish from the other experimental groups (P < 0.05). The results suggested that CDXOS and L. plantarum CR1T5 are viable considerations for potential feed-additive sources.
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http://dx.doi.org/10.1007/s12602-019-09554-5DOI Listing
June 2020

Effects of Cordyceps militaris spent mushroom substrate on mucosal and serum immune parameters, disease resistance and growth performance of Nile tilapia, (Oreochromis niloticus).

Fish Shellfish Immunol 2017 Aug 31;67:78-85. Epub 2017 May 31.

Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200 Thailand.

The aim of present study was determination effects of dietary administration of C. militaris spent mushroom substrate (SMS) on mucosal and serum immune parameters, disease resistance, and growth performance of Nile tilapia (Oreochromis niloticus). Two hundred twenty five fish of similar weight (37.28 ± 0.10 g) were assigned to the following diets [0 (T1- Control), 5 (T2), 10 (T3), 20 (T4) and 40 g kg (T5) SMS]. After 60 days of feeding trial, growth performance, skin mucus lysozyme and peroxidase activities as well as serum innate immune were measured. In addition, survival rate and innate immune responses were calculated after challenge test (15 days) against Streptococcus agalactiae. The results revealed that regardless of inclusion levels, feeding Nile tilapia with SMS supplemented diets significantly increased skin mucus lysozyme and peroxidase activities as well as serum immune parameters (SL, ACH50, PI, RB, and RB) compared control group (P < 0.05). The highest increment of immune parameters was observed in fish fed 10 g kg SMS which was significantly higher than other treatments (P < 0.05). Also, the relative percent survival (RSP) in T2, T3, T4, and T5 was 61.11%, 88.89%, 66.67, and 55.56%, respectively. Among the supplemented groups, fish fed 10 g kg SMS showed significant higher RPS and resistance to S. agalactiae than other groups (P < 0.05). Regarding the growth performance, SGR, WG, FW, and FCR were remarkably improved (P < 0.05) in SMS groups; the highest improvement observed in 10 g kg SMS treatment. According to these finding, administration of 10 g kg SMS is suggested in tilapia to improve growth performance and health status.
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http://dx.doi.org/10.1016/j.fsi.2017.05.062DOI Listing
August 2017

The effects of dietary kefir and low molecular weight sodium alginate on serum immune parameters, resistance against Streptococcus agalactiae and growth performance in Nile tilapia (Oreochromis niloticus).

Fish Shellfish Immunol 2017 Mar 11;62:139-146. Epub 2017 Jan 11.

Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, 50200, Thailand.

The present study evaluates the effects of dietary kefir and low molecular weight sodium alginate (LWMSA) (singular or combined) on non-specific immune response, disease resistance and growth performance of Nile tilapia (Oreochromis niloticus). Fish with average weight of 18.60 ± 0.04 g were supplied and randomly stocked in sixteen glass tanks (150 L) at density of 20 fish per tank. Fish were fed experimental diets as follows: 0 g kg LMWSA (Control, Diet 1), 10 g kg LMWSA (Diet 2), 40 g kg kefir (Diet 3), and 10 g kg LMWSA + 40 g kg kefir (Diet 4) for 50 days. At the end of the feeding trial, serum lysozyme (SL), phagocytosis (PI), respiratory burst (RB), and alternative complement (ACH50) activities as well as growth performance were measured. Singular and combined administration of kefir and low molecular weight sodium alginate (LMWSA) significantly increased serum SL, PI, RB, and ACH50 activities compared control group (P < 0.05); the highest innate immune responses were observed in fish fed combinational diet (kefir + LMWSA) (P < 0.05). The results of experimental challenge revealed significantly higher resistance against Streptococcus agalactiae in fish fed supplemented diets and the highest post challenge survival rate was observed in synbiotic diet (P < 0.05). Similar results obtained in case of growth parameters. Feeding on supplemented diet significantly improved SGR and FCR and the highest growth parameters was observed in fish fed synbiotic diet (P < 0.05). These finding revealed that combined administration of dietary kefir and LMWSA can be considered for improving immune response, disease resistance and growth performance of Nile tilapia.
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http://dx.doi.org/10.1016/j.fsi.2017.01.014DOI Listing
March 2017

Combined administration of low molecular weight sodium alginate boosted immunomodulatory, disease resistance and growth enhancing effects of Lactobacillus plantarum in Nile tilapia (Oreochromis niloticus).

Fish Shellfish Immunol 2016 Nov 11;58:678-685. Epub 2016 Oct 11.

Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand.

The present study investigates the effects of combined or singular administration of low molecular weight sodium alginate (LWMSA) and Lactobacillus plantarum on innate immune response, disease resistance and growth performance of Nile tilapia (Oreochromis niloticus). Three hundred and twenty fish were supplied and randomly stocked in sixteen glass tanks (150 L) assigned to four treatments as follows: 0 g kg LMWSA (Control, Diet 1), 10 g kg LMWSA (Diet 2), 10 CFU gL. plantarum (Diet 3), and 10 g kg LMWSA + 10 CFU gL. plantarum (Diet 4). Following 30 and 60 days of the feeding trial, serum lysozyme, phagocytosis, respiratory burst and alternative complement activities as well as growth performance parameters (specific growth rate, feed conversion ratio) were measured. Serum lysozyme, phagocytosis, respiratory burst, and alternative complement activities of fish were significantly stimulated by both LMWSA and L. plantarum diets, however, the highest innate immune response were observed in fish fed synbiotic diet. At the end of the experiment, eight fish per replication were randomly selected for a challenge test against Streptococcus agalactiae. The survival rate of the fish fed supplemented diets was significantly greater than the control treatment and the highest post challenge survival rate was observed in synbiotic diet. Furthermore, SGR and FCR were significantly improved in fish fed supplemented diets after 60 days and the highest growth performance was observed in fish fed synbiotic diet. These results suggest combined LMWSA and L. plantarum can be considered as a promising immunostimulant and growth enhancer in Nile tilapia diet.
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http://dx.doi.org/10.1016/j.fsi.2016.10.013DOI Listing
November 2016

Effects of low molecular weight sodium alginate on growth performance, immunity, and disease resistance of tilapia, Oreochromis niloticus.

Fish Shellfish Immunol 2016 Aug 26;55:186-94. Epub 2016 May 26.

Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand.

Present study was carried out to evaluate the effects of low molecular weight sodium alginate (LMWSA) as potential prebiotic source on growth performance, innate immunity and disease resistance of tilapia, Oreochromis niloticus. Three hundred twenty fish were divided into four treatments and fed following diets 0 (T1- Control), 10 (T2), 20 (T3) and 30 (T4) g kg(-1) LMWSA for period of 60 days. A Completely Randomized Design with four replications was applied. At the end of experiment, fish in each replication were weighed and specific growth rate (SGR) and feed conversion ratio (FCR) were calculated. Five randomly selected fish were used for innate immune response measurement. Another ten fish were randomly selected for challenge test against Streptococcus agalactiae for a period of 18 days. The lysozyme, complement, phagocytosis, and respiratory burst activities were detected after 60 days of feeding trial and after challenge test. The results indicated that fish fed diet 10 g kg(-1) LMWSA significantly improved SGR and FCR after 60 days of feeding trial. The lysozyme, phagocytosis, respiratory burst, and complement activities were significantly higher in fish fed LMWSA diets compared to control. Fish fed 10 g kg(-1) LMWSA had greatest values compared to fish fed 20 and 30 g kg(-1) LMWSA. The survival rate of O. niloticus was significantly improved in fish fed LMWSA diets after challenge with S. agalactiae for 18 days. However, no significant difference in survival rate was observed among LMWSA supplemented diets. It is indicated that fish fed 10, 20 and 30 g kg(-1) LMWSA diets can stimulate growth performance, innate immunity and disease resistance in tilapia against S. agalactiae.
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http://dx.doi.org/10.1016/j.fsi.2016.05.034DOI Listing
August 2016

Natrinema gari sp. nov., a halophilic archaeon isolated from fish sauce in Thailand.

Int J Syst Evol Microbiol 2008 Oct;58(Pt 10):2378-83

Department of Food Technology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.

Two Gram-negative, rod-shaped, halophilic archaea, designated strains HIS40-3(T) and HDS3-1, were isolated from anchovy fish sauce (nam-pla) collected from two different locations in Thailand. The two strains were able to grow at 20-60 degrees C (optimum 37-40 degrees C), at 1.7-5.1 M NaCl (optimum 2.6-3.4 M NaCl) and at pH 5.5-8.5 (optimum pH 6.0-6.5). Hypotonic treatment with less than 1.7 M NaCl caused cell lysis. The major polar lipids of the isolates were C(20)C(20) and C(20)C(25) derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate, two glycolipids and one unidentified lipid. The DNA G+C contents were 64.0-65.4 mol%. In addition to phenotypic and chemotaxonomic characteristics, phylogenetic analysis based on 16S rRNA gene sequence similarities showed that strains HIS40-3(T) and HDS3-1 were related most closely to species of the genus Natrinema. Levels of 16S rRNA gene sequence similarity between strains HIS40-3(T) and HDS3-1 and the type strains of recognized Natrinema species were 99.1-96.6 %. The two novel strains could be distinguished from recognized Natrinema species on the basis of low levels of DNA-DNA relatedness and differences in whole-cell protein patterns and phenotypic properties. Levels of 16S rRNA gene sequence similarity and DNA-DNA relatedness between the two strains were 99.7 and 77.7 %, respectively, suggesting that they should be classified as representing a single species. Based on these taxonomic data, strains HIS40-3(T) and HDS3-1 are considered to represent a novel species of the genus Natrinema, for which the name Natrinema gari sp. nov. is proposed. The type strain is HIS40-3(T) (=BCC 24370(T) =JCM 14663(T) =PCU 303(T)).
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http://dx.doi.org/10.1099/ijs.0.65644-0DOI Listing
October 2008