Publications by authors named "Vladyslav Mishyn"

6 Publications

  • Page 1 of 1

The role of the surface ligand on the performance of electrochemical SARS-CoV-2 antigen biosensors.

Anal Bioanal Chem 2021 Feb 22. Epub 2021 Feb 22.

Univ. Lille, CNRS, Centrale Lille, Univ. Polytechnique Hauts-de-France, UMR 8520 - IEMN, F-59000, Lille, France.

Point-of-care (POC) technologies and testing programs hold great potential to significantly improve diagnosis and disease surveillance. POC tests have the intrinsic advantage of being able to be performed near the patient or treatment facility, owing to their portable character. With rapid results often in minutes, these diagnostic platforms have a high positive impact on disease management. POC tests are, in addition, advantageous in situations of a shortage of skilled personnel and restricted availability of laboratory-based analytics. While POC testing programs are widely considered in addressing health care challenges in low-income health systems, the ongoing pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections could largely benefit from fast, efficient, accurate, and cost-effective point-of-care testing (POCT) devices for limiting COVID-19 spreading. The unrestrained availability of SARS-CoV-2 POC tests is indeed one of the adequate means of better managing the COVID-19 outbreak. A large number of novel and innovative solutions to address this medical need have emerged over the last months. Here, we critically elaborate the role of the surface ligands in the design of biosensors to cope with the current viral outbreak situation. Their notable effect on electrical and electrochemical sensors' design will be discussed in some given examples. Graphical abstract.
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http://dx.doi.org/10.1007/s00216-020-03137-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7897554PMC
February 2021

Plasmon-Driven Electrochemical Methanol Oxidation on Gold Nanohole Electrodes.

ACS Appl Mater Interfaces 2020 Nov 29;12(45):50426-50432. Epub 2020 Oct 29.

Mishyn Univ. Lille, CNRS, Centrale Lille, Univ. Polytechnique Hauts-de-France, UMR 8520-IEMN, F-59000 Lille, France.

Direct methanol oxidation is expected to play a central role in low-polluting future power sources. However, the sluggish and complex electro-oxidation of methanol is one of the limiting factors for any practical application. To solve this issue, the use of plasmonic is considered as a promising way to accelerate the methanol oxidation reaction. In this study, we report on a novel approach for achieving enhanced methanol oxidation currents. Perforated gold thin film anodes were decorated with Pt/Ru via electrochemical deposition and investigated for their ability for plasmon-enhanced electrocatalytic methanol oxidation in alkaline media. The novel methanol oxidation anode (AuNHs/PtRu), combining the strong light absorption properties of a gold nanoholes array-based electrode (AuNHs) with surface-anchored bimetallic Pt/Ru nanostructures, known for their high activity toward methanol oxidation, proved to be highly efficient in converting methanol via the hot holes generated in the plasmonic electrode. Without light illumination, AuNHs/PtRu displayed a maximal current density of 13.7 mA/cm at -0.11 V vs Ag/AgCl. Enhancement to 17.2 mA/cm was achieved under 980 nm laser light illumination at a power density of 2 W/cm. The thermal effect was negligible in this system, underlining a dominant plasmon process. Fast generation and injection of charge carriers were also evidenced by the abrupt change in the current density upon laser irradiation. The good stability of the interface over several cycles makes this system interesting for methanol electro-oxidation.
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http://dx.doi.org/10.1021/acsami.0c14436DOI Listing
November 2020

Electronic biosensors based on graphene FETs.

Methods Enzymol 2020 20;642:371-401. Epub 2020 Jul 20.

CEST Competence Center for Electrochemical Surface Technology, Tulln, Austria; AIT Austrian Institute of Technology GmbH, Biosensor Technologies, Tulln, Austria. Electronic address:

Olfaction is capable of accomplishing incredible tasks: it starts with capturing an odor molecule, delivering it to the odorant receptors, converting it into an electrical stimulus and transmitting the data to the brain. And all of this in milliseconds. The sense of smell is not yet fully decoded and is far from being replicated by modern sensor technologies. One approach to convert biological recognition- and binding events in real-time and in a label-free manner to electrical signals is emulated in a "biomimetic electronic smell sensor". It is based on a transistor, in many cases realized as a field-effect transistor (FET) with a biorecognition element, e.g., an odorant binding protein (OBP) converting the binding event of one of its typically many ligands directly into a measurable electrical signal. OBPs are immobilized on these FETs and modulate the current in the presence of smell molecules due to the charge redistribution in the gated channel. Graphene is an elegant candidate to realize such a sensor device because an atomic monolayer of a semiconducting material leads to increased sensitivity. Beside the direct molecule interaction with the substrate upon binding and its excellent biocompatible character, graphene has the advantage of a biological-friendly working point in the sub-Volt regime. Different approaches of preparation and functionalization of graphene field-effect transistors (gFETs) are utilized to tune the performance for odorant sensing. The evaluation of kinetic binding parameters like association and dissociation rate constants and the equilibrium affinity constants of protein-ligand interactions can be derived from the direct electrical read-out of such miniaturized sensor systems. In this article, the state of the art of gFET preparation, functionalization, and operation for odorant sensing will be discussed.
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http://dx.doi.org/10.1016/bs.mie.2020.05.016DOI Listing
June 2021

Reduced graphene oxide-based field effect transistors for the detection of E7 protein of human papillomavirus in saliva.

Anal Bioanal Chem 2021 Jan 20;413(3):779-787. Epub 2020 Aug 20.

Univ. Lille, CNRS, Centrale Lille, Univ. Polytechnique Hauts-de-France, UMR 8520 - IEMN, 59000, Lille, France.

Several challenging biological sensing concepts have been realized using electrolyte-gated reduced graphene oxide field effect transistors (rGO-FETs). In this work, we demonstrate the interest of rGO-FET for the sensing of human papillomavirus (HPV), one of the most common sexually transmitted viruses and a necessary factor for cervical carcinogenesis. The highly sensitive and selective detection of the HPV-16 E7 protein relies on the attractive semiconducting characteristics of pyrene-modified rGO functionalized with RNA aptamer Sc5-c3. The aptamer-functionalized rGO-FET allows for monitoring the aptamer-HPV-16 E7 protein binding in real time with a detection limit of about 100 pg mL (1.75 nM) for HPV-16 E7 from five blank noise signals (95% confidence level). The feasibility of this method for clinical application in point-of-care technology is evaluated using HPV-16 E7 protein suspended in saliva and demonstrates the successful fabrication of a promising field effect transistor biosensor for HPV diagnosis.Graphical abstract.
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http://dx.doi.org/10.1007/s00216-020-02879-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7438402PMC
January 2021

Dopamine-functionalized cyclodextrins: modification of reduced graphene oxide based electrodes and sensing of folic acid in human serum.

Anal Bioanal Chem 2019 Aug 27;411(20):5149-5157. Epub 2019 Jun 27.

Université de Lille, CNRS, Centrale Lille, ISEN, Université de Valenciennes, UMR 8520-IEMN, 59000, Lille, France.

A mandatory step in any sensor fabrication is the introduction of analyte-specific recognition elements to the transducer surface. In this study, the possibility to anchor β-cyclodextrin-modified dopamine to a reduced graphene oxide based electrochemical transducer for the sensitive and selective sensing of folic acid is demonstrated. The sensor displays good electrocatalytic activity toward the oxidation of folic acid. The strong affinity of the surface-confined β-cyclodextrin for folic acid, together with favorable electron transfer characteristics, resulted in a sensor with a detection limit of 1 nM for folic acid and a linear response up to 10 μM. Testing of the sensor on serum samples from healthy individuals and patients diagnosed with folic acid deficiency validated the sensing capability. Graphical abstract.
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http://dx.doi.org/10.1007/s00216-019-01892-1DOI Listing
August 2019

Electrochemical cardiovascular platforms: Current state of the art and beyond.

Biosens Bioelectron 2019 Apr 19;131:287-298. Epub 2019 Feb 19.

Univ. Lille, CNRS, Centrale Lille, ISEN, Univ. Valenciennes, UMR 8520-IEMN, F-59000 Lille, France. Electronic address:

Cardiovascular diseases (CVD) remain the leading cause of death within industrialized nations as well as an increasing cause of mortality and morbidity in many developing countries. Smoking, alcohol consumption and increased level of blood cholesterol are the main CVD risk factors. Other factors, such as the prevalence of overweight/obesity and diabetes, have increased considerably in recent decades and are indirect causes of CVD. Among CVDs, the acute coronary syndrome (ACS) represents the most common cause of emergency hospital admission. Since the prognosis of ACS is directly associated with timely initiation of revascularization, missed, misdiagnosis or late diagnosis have unfavorable medical implications. Early ACS diagnosis can reduce complications and risk of recurrence, finally decreasing the economic burden posed on the health care system as a whole. To decrease the risk of ACS and related CVDs and to reduce associated costs to healthcare systems, a fast management of patients with chest pain has become crucial and urgent. Despite great efforts, biochemical diagnostic approaches of CVDs remain difficult and controversial medical challenges as cardiac biomarkers should be rapidly released into the blood at the time of ischemia and persistent for a sufficient length of time to allow diagnostics, with tests that should be rapid, easy to perform and relatively inexpensive. Early biomarker assessments have involved testing for the total enzyme activity of aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and creatine kinase (CK), which cardiac troponins being the main accepted biomarkers for diagnosing myocardial injury and acute myocardial infarction (AMI). To allow rapid diagnosis, it is necessary to replace the traditional biochemical assays by cardiac biosensor platforms. Among the numerous of possibilities existing today, electrochemical biosensors are important players as they have many of the required characteristics for point-of-care tests. Electrochemical based cardiac biosensors are highly adapted for monitoring the onset and progress of cardiovascular diseases in a fast and accurate manner, while being cheap and scalable devices. This review outlines the state of the art in the development of cardiac electrochemical sensors for the detection of different cardiac biomarkers ranging from troponin to BNP, N-terminal proBNP, and others.
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http://dx.doi.org/10.1016/j.bios.2019.02.010DOI Listing
April 2019