Publications by authors named "Vladimir V Muzyka"

3 Publications

  • Page 1 of 1

Natural and Artificial Mechanisms of Mitochondrial Genome Elimination.

Life (Basel) 2021 Jan 20;11(2). Epub 2021 Jan 20.

Federal Research Center Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia.

The generally accepted theory of the genetic drift of mitochondrial alleles during mammalian ontogenesis is based on the presence of a selective bottleneck in the female germline. However, there is a variety of different theories on the pathways of genetic regulation of mitochondrial DNA (mtDNA) dynamics in oogenesis and adult somatic cells. The current review summarizes present knowledge on the natural mechanisms of mitochondrial genome elimination during mammalian development. We also discuss the variety of existing and developing methodologies for artificial manipulation of the mtDNA heteroplasmy level. Understanding of the basics of mtDNA dynamics will shed the light on the pathogenesis and potential therapies of human diseases associated with mitochondrial dysfunction.
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http://dx.doi.org/10.3390/life11020076DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7909434PMC
January 2021

Two Separate Cases: Complex Chromosomal Abnormality Involving Three Chromosomes and Small Supernumerary Marker Chromosome in Patients with Impaired Reproductive Function.

Genes (Basel) 2020 12 17;11(12). Epub 2020 Dec 17.

Institute of Cytology and Genetics, The Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia.

For medical genetic counseling, estimating the chance of a child being born with chromosome abnormality is crucially important. Cytogenetic diagnostics of parents with a balanced karyotype are a special case. Such chromosome rearrangements cannot be detected with comprehensive chromosome screening. In the current paper, we consider chromosome diagnostics in two cases of chromosome rearrangement in patients with balanced karyotype and provide the results of a detailed analysis of complex chromosomal rearrangement (CCR) involving three chromosomes and a small supernumerary marker chromosome (sSMC) in a patient with impaired reproductive function. The application of fluorescent in situ hybridization, microdissection, and multicolor banding allows for describing analyzed karyotypes in detail. In the case of a CCR, such as the one described here, the probability of gamete formation with a karyotype, showing a balance of chromosome regions, is extremely low. Recommendation for the family in genetic counseling should take into account the obtained result. In the case of an sSMC, it is critically important to identify the original chromosome from which the sSMC has been derived, even if the euchromatin material is absent. Finally, we present our view on the optimal strategy of identifying and describing sSMCs, namely the production of a microdissectional DNA probe from the sSMC combined with a consequent reverse painting.
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http://dx.doi.org/10.3390/genes11121511DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7766715PMC
December 2020

Avian Influenza Virus with Hemagglutinin-Neuraminidase Combination H8N8, Isolated in Russia.

Genome Announc 2014 Jun 5;2(3). Epub 2014 Jun 5.

Novosibirsk State University, Novosibirsk, Novosibirsk Region, Russia.

We report the genome sequence of an avian influenza virus (AIV) subtype H8N8, isolated in Russia. The genome analysis shows that all genes belong to AIV Eurasian lineages. The PB2 gene was similar to a Mongolian low-pathogenic (LP) AIV H7N1 and a Chinese high-pathogenic (HP) AIV H5N2.
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http://dx.doi.org/10.1128/genomeA.00545-14DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4047453PMC
June 2014