Publications by authors named "Viktor Novik"

2 Publications

  • Page 1 of 1

Conifer Green Needle Complex in Patients with Precancerous Gastric Lesions: An Observational Pilot Study.

Evid Based Complement Alternat Med 2016 28;2016:3848409. Epub 2016 Nov 28.

Solagran Limited, Biotechnology Company, 98-106 Moray St., South Melbourne, VIC 3205, Australia; Saint Petersburg State Forest Technical Academy, Saint Petersburg, Russia.

. infection is common and can lead to precancerous gastric lesions. Standard antibiotic therapy has a failure rate of more than 25% from antibiotic resistance. The primary aim of this observational pilot study was to test the feasibility of a large-scale clinical trial of Conifer Green Needle Complex (CGNC) to treat precancerous gastric lesions. Secondary aims were to investigate infection, stomach function, and histopathology of the gastric mucosa. . A tablet form of CGNC (extracted from and (L) Karst) was prescribed to 26 patients with precancerous gastric lesions (two tablets, 100 mg CGNC/tablet, three times per day for six months). Another 24 patients received no treatment. Compared with control patients, CGNC-treated patients showed total or partial regression (using the quantitative Rome III diagnostic criteria) of dyspeptic symptoms (92.3%, < 0.0001), eradication of infection (57.1%, < 0.03), a reduction in endoscopic signs of gastritis (92.3%, < 0.001), an increase of pepsinogen-pepsin in the gastric juice (57.7%, < 0.05), and total regression or reduction in the degree of intestinal metaplasia (46.2%, < 0.05) and lymphoplasmacytic infiltration (53.8%, < 0.05). This study justifies a randomised-controlled trial with CGNC in patients with atrophic gastritis.
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November 2016

Detection of EGFR mutations and EML4-ALK rearrangements in lung adenocarcinomas using archived cytological slides.

Cancer Cytopathol 2013 Jul 13;121(7):370-6. Epub 2013 Feb 13.

Laboratory of Molecular Oncology, N.N. Petrov Institute of Oncology, St. Petersburg, Russia.

Background: Although the molecular analysis of epidermal growth factor receptor (EGFR) and anaplastic lymphoma kinase (ALK) in archived lung cancer tissues is relatively well established, the genetic testing of cytological material has not yet become a routine.

Methods: The current study used cell samples that were obtained by bronchial brushing, transthoracic needle aspiration, or biopsy imprint preparation between 1993 and 2008. Islets of malignant cells were visually located on the archived cytological slides, lysed in situ by a drop of sodium dodecyl sulfate-containing buffer, and subjected to the standard DNA and RNA extraction. Examination of paraffin-embedded tissue blocks (resection specimens or biopsy material) from the same patients was performed in parallel.

Results: A total of 75 cytological/histological lung adenocarcinoma sample pairs underwent polymerase chain reaction analysis for the EGFR mutation. Two cytological samples and 1 morphological sample failed to produce DNA. Concordance for the wild-type and mutation status was observed in 54 of 72 and 14 of 72 informative pairs, respectively; 3 pairs and 1 pair, respectively, had mutation only in the cytological or histological material. The discrepancies could be explained by the failure to ensure a high percentage of lung cancer cells in the analyzed samples or, alternatively, by the genuine intratumoral molecular heterogeneity of some neoplasms. RNA extraction followed by reverse transcriptase-polymerase chain reaction analysis for the EML4-ALK translocation was performed for 44 EGFR mutation-negative sample pairs; failures were observed for 2 cytological and 6 histological specimens. All informative pairs were concordant either for the norm (32 of 36 pairs) or for the presence of EML4-ALK gene fusion (4 of 36 pairs).

Conclusions: Archived cytological slides appear to be well suited both for EGFR and ALK analysis.
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July 2013