Publications by authors named "Vijaya Ramu Dirisala"

3 Publications

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Extraction of bioactive compounds from Psidium guajava leaves and its utilization in preparation of jellies.

AMB Express 2021 Mar 1;11(1):36. Epub 2021 Mar 1.

Department of Biotechnology, Vignan's Foundation for Science, Technology and Research, Vadlamudi, Andhra Pradesh, 522213, India.

Psidium guajava L. (guava) is predominantly grown throughout the world and known for its medicinal properties in treating various diseases and disorders. The present work focuses on aqueous extraction of bioactive compounds from the guava leaf and its utilization in the formulation of jelly to improve the public health. The guava leaf extract has been used in the preparation of jelly with pectin (1.5 g), sugar (28 g) and lemon juice (2 mL). The prepared guava leaf extract jelly (GJ) and the control jelly (CJ, without extract) were subjected to proximate, nutritional and textural analyses besides determination of antioxidant and antimicrobial activities. GJ was found to contain carbohydrate (45.78 g/100 g), protein (3.0 g/100 g), vitamin C (6.15 mg/100 g), vitamin B3 (2.90 mg/100 g) and energy (120.6 kcal). Further, the texture analysis of CJ and GJ indicated that both the jellies showed similar properties emphasizing that the addition of guava leaf extract does not bring any change in the texture properties of jelly. GJ exhibited antimicrobial activity against various bacteria ranging from 11.4 to 13.6 mm. Similarly, GJ showed antioxidant activity of 42.38% against DPPH radical and 33.45% against hydroxyl radical. Mass spectroscopic analysis of aqueous extract confirmed the presence of esculin, quercetin, gallocatechin, 3-sinapoylquinic acid, gallic acid, citric acid and ellagic acid which are responsible for antioxidant and antimicrobial properties.
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http://dx.doi.org/10.1186/s13568-021-01194-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921237PMC
March 2021

Extraction of bioactive compounds from Psidium guajava and their application in dentistry.

AMB Express 2019 Dec 28;9(1):208. Epub 2019 Dec 28.

Department of Biotechnology, Vignan's Foundation for Science, Technology and Research, Guntur, Andhra Pradesh, 522213, India.

Guava is considered as poor man's apple rich in phytochemicals with medicinal value and hence it is highly consumed. Gas chromatography-mass spectroscopy (GC-MS) analysis of guava leaf extract revealed the presence of various bioactive compounds with antimicrobial, antioxidant, anticancer, and antitumor properties. Hence, it is used in tooth paste formulations along with other ingredients such as Acacia arabica gum powder, stevia herb powder, sea salt, extra virgin coconut oil, peppermint oil in the present study. Three formulations F1, F2 and F3 have been made by varying the concentration of these ingredients and the prepared formulations were studied for their antimicrobial activity and physico-chemical parameters such as pH, abrasiveness, foaming activity, spreading and cleaning ability. Among these, F3 showed significant antioxidant and antimicrobial properties, minimal cytotoxicity, maximum spreadability and very high cleaning ability. This study surmises that the herbal toothpaste formulation is greener, rich in medicinal values and imparts oral hygiene.
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http://dx.doi.org/10.1186/s13568-019-0935-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6935383PMC
December 2019

An efficient method for integration of PCR fragments into adjacent or overlapping restriction sites during gene cloning.

3 Biotech 2018 Apr 24;8(4):197. Epub 2018 Mar 24.

Department of Biotechnology, Vignan's Foundation for Science, Technology and Research (VFSTR), Vadlamudi, Guntur District, Andhra Pradesh 522213 India.

In the present work, a simple and straightforward method was developed to clone any PCR-amplified products into restriction sites that are very close, adjacent or overlapping in the expression vector. The novelty of the methodology involves a crucial primer-designing step by adding appropriate overhangs to the 5' ends of primers based on the multiple cloning sites (MCS) (polylinker) region of expression vector. After PCR amplification, actual cloning is performed not in adjacent RE sites, but in sites that are little distant in the MCS. However, the sites lost during this cloning step are maintained intact since they are provided by the cloned PCR product (through the primer overhangs). Gene for green fluorescent protein (GFP) was cloned and expressed employing this strategy to demonstrate its simplicity. This method is highly useful for vector modification without losing the restriction sites present in the MCS.
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http://dx.doi.org/10.1007/s13205-018-1214-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5866252PMC
April 2018