Publications by authors named "Valéry Brunel"

27 Publications

  • Page 1 of 1

Soluble Epoxide Hydrolase Inhibition Prevents Experimental Type 4 Cardiorenal Syndrome.

Front Mol Biosci 2020 11;7:604042. Epub 2021 Mar 11.

Normandie University, UNIROUEN, INSERM U1096, FHU REMOD-VHF, Rouen, France.

Cardiovascular diseases (CVD) remain the leading cause of morbimortality in patients with chronic kidney disease (CKD). The aim of this study was to assess the cardiovascular impact of the pharmacological inhibition of soluble epoxide hydrolase (sEH), which metabolizes the endothelium-derived vasodilatory and anti-inflammatory epoxyeicosatrienoic acids (EETs) to dihydroxyeicosatrienoic acid (DHETs), in the 5/6 nephrectomy (Nx) mouse model. Compared to sham-operated mice, there was decrease in EET-to-DHET ratio 3 months after surgery in vehicle-treated Nx mice but not in mice treated with the sEH inhibitor -AUCB. Nx induced an increase in plasma creatinine and in urine albumin-to-creatinine ratio as well as the development of kidney histological lesions, all of which were not modified by -AUCB. In addition, -AUCB did not oppose Nx-induced blood pressure increase. However, AUCB prevented the development of cardiac hypertrophy and fibrosis induced by Nx, as well as normalized the echocardiographic indices of diastolic and systolic function. Moreover, the reduction in endothelium-dependent flow-mediated dilatation of isolated mesenteric arteries induced by Nx was blunted by -AUCB without change in endothelium-independent dilatation to sodium nitroprusside. Inhibition of sEH reduces the cardiac remodelling, and the diastolic and systolic dysfunctions associated with CKD. These beneficial effects may be mediated by the prevention of endothelial dysfunction, independent from kidney preservation and antihypertensor effect. Thus, inhibition of sEH holds a therapeutic potential in preventing type 4 cardiorenal syndrome.
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http://dx.doi.org/10.3389/fmolb.2020.604042DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7991096PMC
March 2021

Preservation of epoxyeicosatrienoic acid bioavailability prevents renal allograft dysfunction and cardiovascular alterations in kidney transplant recipients.

Sci Rep 2021 Feb 12;11(1):3739. Epub 2021 Feb 12.

Department of Pharmacology, Rouen University Hospital, 76000, Rouen, France.

This study addressed the hypothesis that epoxyeicosatrienoic acids (EETs) synthesized by CYP450 and catabolized by soluble epoxide hydrolase (sEH) are involved in the maintenance of renal allograft function, either directly or through modulation of cardiovascular function. The impact of single nucleotide polymorphisms (SNPs) in the sEH gene EPHX2 and CYP450 on renal and vascular function, plasma levels of EETs and peripheral blood monuclear cell sEH activity was assessed in 79 kidney transplant recipients explored at least one year after transplantation. Additional experiments in a mouse model mimicking the ischemia-reperfusion (I/R) injury suffered by the transplanted kidney evaluated the cardiovascular and renal effects of the sEH inhibitor t-AUCB administered in drinking water (10 mg/l) during 28 days after surgery. There was a long-term protective effect of the sEH SNP rs6558004, which increased EET plasma levels, on renal allograft function and a deleterious effect of K55R, which increased sEH activity. Surprisingly, the loss-of-function CYP2C9*3 was associated with a better renal function without affecting EET levels. R287Q SNP, which decreased sEH activity, was protective against vascular dysfunction while CYP2C8*3 and 2C9*2 loss-of-function SNP, altered endothelial function by reducing flow-induced EET release. In I/R mice, sEH inhibition reduced kidney lesions, prevented cardiac fibrosis and dysfunction as well as preserved endothelial function. The preservation of EET bioavailability may prevent allograft dysfunction and improve cardiovascular disease in kidney transplant recipients. Inhibition of sEH appears thus as a novel therapeutic option but its impact on other epoxyfatty acids should be carefully evaluated.
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http://dx.doi.org/10.1038/s41598-021-83274-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7881112PMC
February 2021

An Unusual Cause of Hypokalemia.

Clin Chem 2020 Dec;66(12):1575-1576

Department of General Biochemistry, Rouen University Hospital, Rouen, France.

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http://dx.doi.org/10.1093/clinchem/hvaa150DOI Listing
December 2020

[Acute hemolysis crisis revealed a Wilson disease].

Ann Biol Clin (Paris) 2020 08;78(4):425-432

Service d'hématologie biologique, Institut de biologie clinique, CHU-Hôpitaux de Rouen, France.

Wilson disease is a rare inherited disorder of copper metabolism that affects liver and brain due to copper tissue accumulation. The mechanism involved is based on mutations of the ATP7B gene. Children have predominant hepatic manifestations while adult are more often diagnosed by neurological and psychiatric symptoms. However, others features are tubulopathy, articular disorders and hemolytic anemia. We report the diagnostic of Wilson disease in a 14 years old girl and her sibling after investigation of hemolytic anemia, hepatic insufficiency, and hypophosphatemia.
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http://dx.doi.org/10.1684/abc.2020.1574DOI Listing
August 2020

5/6 nephrectomy induces different renal, cardiac and vascular consequences in 129/Sv and C57BL/6JRj mice.

Sci Rep 2020 01 30;10(1):1524. Epub 2020 Jan 30.

Normandie Univ, UNIROUEN, INSERM U1096, FHU REMOD-VHF, 76000, Rouen, France.

Experimental models of cardiovascular diseases largely depend on the genetic background. Subtotal 5/6 nephrectomy (5/6 Nx) is the most frequently used model of chronic kidney disease (CKD) in rodents. However, in mice, cardiovascular consequences of 5/6 Nx are rarely reported in details and comparative results between strains are scarce. The present study detailed and compared the outcomes of 5/6 Nx in the 2 main strains of mice used in cardiovascular and kidney research, 129/Sv and C57BL/6JRj. Twelve weeks after 5/6 Nx, CKD was demonstrated by a significant increase in plasma creatinine in both 129/Sv and C57BL/6JRj male mice. Polyuria and kidney histological lesions were more pronounced in 129/Sv than in C57BL/6JRj mice. Increase in albuminuria was significant in 129/Sv but not in C57BL/6JRj mice. Both strains exhibited an increase in systolic blood pressure after 8 weeks associated with decreases in cardiac systolic and diastolic function. Heart weight increased significantly only in 129/Sv mice. Endothelium-dependent mesenteric artery relaxation to acetylcholine was altered after 5/6 Nx in C57BL/6JRj mice. Marked reduction of endothelium-dependent vasodilation to increased intraluminal flow was demonstrated in both strains after 5/6 Nx. Cardiovascular and kidney consequences of 5/6 Nx were more pronounced in 129/Sv than in C57BL/6JRj mice.
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http://dx.doi.org/10.1038/s41598-020-58393-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6992698PMC
January 2020

Pharmacological and analytical interference in hormone assays for diagnosis of adrenal incidentaloma.

Ann Endocrinol (Paris) 2019 Sep 29;80(4):250-258. Epub 2019 Jan 29.

Institute for Clinical Biology-General Biochemistry Unit, Rouen University Hospital, 76000 Rouen, France; Inserm U1073, Laboratory of Digestive Tract Environment and Nutrition ADEN, Normandie University, 76000 Rouen, France.

Adrenal incidentaloma refers to an asymptomatic adrenal mass detected through an imaging procedure performed for reasons unrelated to adrenal dysfunction or suspected dysfunction. In general, adrenal incidentalomas are non-functioning adrenal adenomas, but in some cases, may require therapeutic intervention: eg., adrenocortical carcinoma, pheochromocytoma, primary aldosteronism, cortisol hypersecretion, or adrenal insufficiency. Hormone assessment is crucial to characterize adrenal incidentaloma. Nowadays, various hormone assay methods are available, such as immunoassay and mass spectrometry. However, there are several pitfalls that should be considered: e.g., circadian rhythm, gender/age dependency, preanalytical and analytical issues, and drug interactions. Pharmacological or analytical interference can lead to false serum concentrations and may result in misinterpretation of results and thus inappropriate treatment. The purpose of this review was to study the main interferences that may be observed in the different tumor types of adrenal incidentalomas in order to help physicians in their clinical decision-making and for the overall benefit of patients.
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http://dx.doi.org/10.1016/j.ando.2018.11.006DOI Listing
September 2019

Atazanavir urolithiasis without recent intake of atazanavir.

Ann Biol Clin (Paris) 2019 Aug;77(4):459-460

Department of urology, Charles-Nicolle University Hospital, University of Rouen, France.

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http://dx.doi.org/10.1684/abc.2019.1471DOI Listing
August 2019

An Extremely High IgA Value in a Young Child.

Clin Lab 2019 Jul;65(7)

Background: Hyper-IgA is not a rare finding in children although its causes are less reported than hypergamma-globulinemia in other classes of immunoglobulin. However, an isolated hyper-IgA might play a role as a diagnostic marker, in particular in children with an incomplete clinical picture at disease onset.

Results: We reported the case of a 3-year-old girl hospitalized for acute abdominal symptoms and suspicion of ruptured appendicitis. She presented severe inflammatory syndrome and her medical history related recurrent fever episodes. Serum immunoglobulin analysis was not in favor of an infection; indeed, IgA concentration alone increased and reached a surprising extremely high value in a young child (17-fold of the upper reference value).

Conclusions: This case highlights the potential clinical significance of an isolated hyper-IgA that is known to be mostly found in serious diseases in children; it might contribute to reduce the delay in diagnosis and treatment of hyperimmunoglobulinemia D syndrome, an autoinflammatory disease.
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http://dx.doi.org/10.7754/Clin.Lab.2018.181210DOI Listing
July 2019

The neuropeptide 26RFa in the human gut and pancreas: potential involvement in glucose homeostasis.

Endocr Connect 2019 Jul;8(7):941-951

Normandie Univ, UNIROUEN, INSERM U1239, Laboratory of Neuronal and Neuroendocrine Differentiation and Communication (DC2N), Rouen, France.

Objective: Recent studies performed in mice revealed that the neuropeptide 26RFa regulates glucose homeostasis by acting as an incretin and by increasing insulin sensitivity. However, in humans, an association between 26RFa and the regulation of glucose homeostasis is poorly documented. In this study, we have thus investigated in detail the distribution of 26RFa and its receptor, GPR103, in the gut and the pancreas, and determined the response of this peptidergic system to an oral glucose challenge in obese patients.

Design And Methods: Distribution of 26RFa and GPR103 was examined by immunohistochemistry using gut and pancreas tissue sections. Circulating 26RFa was determined using a specific radioimmunoassay in plasma samples collected during an oral glucose tolerance test.

Results: 26RFa and GPR103 are present all along the gut but are more abundant in the stomach and duodenum. In the stomach, the peptide and its receptor are highly expressed in the gastric glands, whereas in the duodenum, ileum and colon they are present in the enterocytes and the goblet cells. In the pancreatic islets, the 26RFa/GPR103 system is mostly present in the β cells. During an oral glucose tolerance test, plasma 26RFa profile is different between obese patients and healthy volunteers, and we found strong positive correlations between 26RFa blood levels and the BMI, and with various parameters of insulin secretion and insulin resistance.

Conclusion: The present data suggest an involvement of the 26RFa/GPR103 peptidergic system in the control of human glucose homeostasis.
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http://dx.doi.org/10.1530/EC-19-0247DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6612231PMC
July 2019

Neuropeptide 26RFa (QRFP) is a key regulator of glucose homeostasis and its activity is markedly altered in obese/hyperglycemic mice.

Am J Physiol Endocrinol Metab 2019 07 14;317(1):E147-E157. Epub 2019 May 14.

Normandie University, UNIROUEN, INSERM U1239, Laboratory of Neuronal and Neuroendocrine Differentiation and Communication (DC2N) , Rouen , France.

Recent studies have shown that the hypothalamic neuropeptide 26RFa regulates glucose homeostasis by acting as an incretin and increasing insulin sensitivity. In this study, we further characterized the role of the 26RFa/GPR103 peptidergic system in the global regulation of glucose homeostasis using a 26RFa receptor antagonist and also assessed whether a dysfunction of the 26RFa/GPR103 system occurs in obese hyperglycemic mice. First, we demonstrate that administration of the GPR103 antagonist reduces the global glucose-induced incretin effect and insulin sensitivity whereas, conversely, administration of exogenous 26RFa attenuates glucose-induced hyperglycemia. Using a mouse model of high-fat diet-induced obesity and hyperglycemia, we found a loss of the antihyperglcemic effect and insulinotropic activity of 26RFa, accompanied with a marked reduction of its insulin-sensitive effect. Interestingly, this resistance to 26RFa is associated with a downregulation of the 26RFa receptor in the pancreatic islets, and insulin target tissues. Finally, we observed that the production and release kinetics of 26RFa after an oral glucose challenge is profoundly altered in the high-fat mice. Altogether, the present findings support the view that 26RFa is a key regulator of glucose homeostasis whose activity is markedly altered under obese/hyperglycemic conditions.
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http://dx.doi.org/10.1152/ajpendo.00540.2018DOI Listing
July 2019

[Alagille syndrome, lipoprotein X, and false hyponatremia].

Ann Biol Clin (Paris) 2019 06;77(3):353-354

Service de biochimie générale,institut de biologie clinique, CHU de Rouen, France.

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http://dx.doi.org/10.1684/abc.2019.1437DOI Listing
June 2019

An Additional Case of Hb Saint Nazaire [β103(G5)Phe→Ile; : c.310T>A] Leading to Moderate Erythrocytosis in a French Family.

Hemoglobin 2019 Jan 30;43(1):50-51. Epub 2019 Jan 30.

a Service d'Hématologie Biologique, Centre Hospitalier Universitaire de Rouen , Rouen , France.

We report two members of a French family who are carriers of a rare hemoglobin (Hb) variant leading to erythrocytosis: Hb Saint Nazaire [β103(G5)Phe→Ile; : c.310T>A]. The proband is a 38-year-old woman referred to our institution for a moderate but persistent polycythemia without any clinical consequence. As her mother had a similar blood count, a diagnosis of a Hb variant with high oxygen affinity was proposed. The variant was difficult to detect by capillary electrophoresis (CE) and not distinguishable by high performance liquid chromatography (HPLC) and isoelectric focusing. Finally, a heterozygous mutation on the gene corresponding to Hb Saint Nazaire was identified. This case report illustrates that this rare cause of erythrocytosis can be easily under or misdiagnosed unless several Hb separation techniques are used.
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http://dx.doi.org/10.1080/03630269.2019.1567529DOI Listing
January 2019

Biotin and high-sensitivity cardiac troponin T assay.

Biochem Med (Zagreb) 2018 Oct;28(3):030901

General Biochemistry Department, Rouen University Hospital, Rouen, France.

Introduction: The high-sensitivity cardiac troponin T assay of Roche Diagnostics is known to have interference with high concentrations of biotin as this assay uses biotin-streptavidin binding as detection method. As studies so far have not shown if different biotin concentrations could have diverse influence on various troponin concentrations and whether interference could be removed by available protocol within corresponding turnaround time we aimed to investigate it.

Materials And Methods: Plasma samples were spiked with different concentration of biotin solution. Troponin T concentrations were tested on a Roche Cobas® 8000 module 602 analyser. Final concentrations of biotin and troponin T were 50, 100, 500 and 1000 μg/L and 18, 59, 201 and 6423 ng/L, respectively. Impact of different incubation times following biotin neutralization protocol described by Piketty was also tested.

Results: We observed a mean of negative biases of 24, 56, 97, and 98% of the troponin T expected value at biotin concentrations of 50, 100, 500, 1000 μg/L. Neutralization protocol was applied on the sample with initial concentration of TnT of 59 ng/L at a biotin concentration of 1000 μg/L. Same results across different incubation times from 60 to 0 minutes were obtained (mean value 56.8 ng/L, coefficient of variation of 1.31%). We demonstrated that neutralization process had a dilution effect of the troponin concentration (loss of 4.5% to 9.6% of initial troponin value).

Conclusions: Biotin interference is not dependent of initial troponin value. Interference could be successfully neutralized within a time frame compatible with emergency but results still should be carefully interpreted due to possible dilution effect.
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http://dx.doi.org/10.11613/BM.2018.030901DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214697PMC
October 2018

The suitable prescription of the thyroid blood test in the diagnosis of dysthyroidism: a retrospective study in Rouen University Hospital.

Ann Biol Clin (Paris) 2018 Aug;76(4):421-428

Service de biochimie générale, Institut de biologie clinique, CHU de Rouen, France, Inserm UMR 1073, Faculté de médecine et de pharmacie, Université de Rouen, France.

The thyroid blood test (TSH, FT4, FT3) is often prescribed. This test follows specific French guide-lines. The aim of this work was to study whether its prescription, as part of the initial diagnosis of dysthyroidism, complied with the official recommendations at Rouen University Hospital. We also evaluated the evolution of its prescription between 2014 and 2017 at Rouen University Hospital. A descriptive retrospective study was performed on patients who had undergone a thyroid blood test from June 1st, 2017 to June 29th, 2017. Among the 1,143 thyroid blood test used to assess the suitable prescription of the thyroid blood test, 143 did not respect the guide-lines (12.5%). Among the 143 thyroid blood test, 108 (or 75.5%) came from consultation or day hospital units. The significant percentage of thyroid tests that did not comply with the guide-lines is mainly due to the achievement of a "complete thyroid blood test" for reasons of convenience.
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http://dx.doi.org/10.1684/abc.2018.1359DOI Listing
August 2018

Atypical Pseudohyponatremia.

Clin Chem 2018 02;64(2):414-415

Department of Medical Biochemistry, Rouen University Hospital, Rouen, France.

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http://dx.doi.org/10.1373/clinchem.2017.276501DOI Listing
February 2018

Dipotassium ethylenediaminetetraacetic acid is better than tripotassium salt for electrochemiluminescence insulin measurement.

Clin Chim Acta 2016 Dec 29;463:45-46. Epub 2016 Sep 29.

Medical Biochemistry, Rouen University Hospital, Rouen, France, 1 rue de Germont, 76031 Rouen, France. Electronic address:

Previous studies reported that stability of insulin was better on ethylenediaminetetraacetic acid (EDTA) plasma sample than serum sample. However, those studies used tripotassium EDTA (K3-EDTA) tubes, rather than dipotassium EDTA (K2-EDTA) tubes which are more commonly used in laboratories. We investigated the impact of preservative type of EDTA (K2 or K3) on the stability of C-peptide and insulin at 4°C and at room temperature room. Our study has identified that K2-EDTA achieves longer stability of insulin but does not improve the stability of C-peptide. Insulin and C-peptide are stable 24h on the same K2-EDTA sample at room temperature.
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http://dx.doi.org/10.1016/j.cca.2016.09.014DOI Listing
December 2016

Haemoglobin J-Baltimore can be detected by HbA1c electropherogram but with underestimated HbA1c value.

Biochem Med (Zagreb) 2016 ;26(2):240-2

Department of Medical Biochemistry, Rouen University Hospital, Rouen, France; Department of Pharmacology, Rouen University Hospital, Rouen, France.

Glycated haemoglobin (HbA(1c)) is considered the gold standard for assessing diabetes compensation and treatment. In addition, fortuitous detection of haemoglobin variants during HbA1c measurement is not rare. Recently, two publications reported different conclusions on accuracy of HbA(1c) value using capillary electrophoresis method in presence of haemoglobin J-Baltimore (HbJ).
Here we describe the fortuitous detection of unknown HbJ using capillary electrophoresis for measurement of HbA(1c). A patient followed for gestational diabetes in our laboratory presented unknown haemoglobin on Capillarys 2 Flex Piercing analyser which was identified as HbJ. HbJ is not associated with haematological abnormalities. High Performance Liquid Chromatography methods are known to possibly underestimate HbA(1c) value in the presence of this variant. This variant and its glycated form are clearly distinguished on electropherogram but HbJ was responsible for underestimating the true area of HbA(1c).
 Capillary electrophoresis is a good method for detecting HbJ but does not seem suitable for evaluation of HbA(1C) value in patients in presence of HbJ variant.
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http://dx.doi.org/10.11613/BM.2016.026DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4910267PMC
August 2016

Prognostic value of PCT in septic emergency patients.

Ann Intensive Care 2016 Dec 21;6(1):47. Epub 2016 May 21.

Department of Emergency Medicine, Hôpitaux Universitaires Est Parisien - Hôpital Tenon, Assistance Publique des Hôpitaux de Paris (AP-HP), 4 rue de la Chine, 75020, Paris, France.

Background: An accurate assessment of septic patients at risk for poor clinical outcomes is challenging for clinicians in the emergency department (ED).

Objectives: We aimed to evaluate the prognostic value of procalcitonin (PCT) in septic patients in the ED for predicting death.

Results: In a retrospective study, 188 septic patients (median age 63 [IQR 51-80]) of two French university hospitals were included. Patients who deceased within 30 days (20 %, n = 37) presented higher PCT value at admission (median 34.0 µg/L [5.0-71.9]) in comparison with the survivals (median 6.4 µg/L [4.1-13.1], p = 0.0005). ROC curve analysis indicated a moderate AUC of 0.686 [95 % CI 0.613-0.752] and an optimal PCT threshold value at 32.5 [95 % CI 21.8-43.3] µg/L that was associated with a 51 % [34-67] sensitivity, a 96 % [90-98] specificity, a 73 % [52-88] positive predictive value, and a 89 % [83-93] negative predictive value for death. Only 26 patients (14 %) had PCT values above this threshold (19 in the deceased group vs 7 in survival group, p < 0.0001). By multivariate analysis, only three variables remained significantly predictive of the death: personal history of cardiovascular disease (OR 3.1 [1.0-9.4], p = 0.046), the presence of severe sepsis/septic shock in the ER (OR 4.4 [1.3-12.3], p = 0.013), and a PCT level >32.5 µg/L (OR 36.0 [10.0-128.4], p < 0.0001). Similar results were obtained when considering the combined outcome death and/or admission in ICU.

Conclusion: Elevated value of PCT at admission has moderate accuracy to identify poor outcome in ED septic patients in daily practice.
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http://dx.doi.org/10.1186/s13613-016-0146-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4875576PMC
December 2016

Chronically Hypocalcemic Patient with Hypercalcemia.

Clin Chem 2016 05;62(5):783-4

Department of Medical Biochemistry, and Department of Pharmacology, Rouen University Hospital, Rouen, France.

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http://dx.doi.org/10.1373/clinchem.2015.247023DOI Listing
May 2016

NMDA receptor blockade in the developing cortex induces autophagy-mediated death of immature cortical GABAergic interneurons: An ex vivo and in vivo study in Gad67-GFP mice.

Exp Neurol 2015 May 17;267:177-93. Epub 2015 Mar 17.

Region-Inserm Team NeoVasc ERI28, Laboratory of Microvascular Endothelium and Neonate Brain Lesions, Institute of Research for Innovation in Biomedicine, Normandy University, Rouen, France; Department of Medical Biochemistry, Rouen University Hospital, Rouen, France.

In neonates, excitotoxicity is a major process involved in hypoxic-ischemic brain lesions, and several research groups have suggested the use of NMDA antagonists for neuroprotection. However, despite their clinical interest, there is more and more evidence suggesting that, in the immature brain, these molecules exert deleterious actions on migrating GABAergic interneurons by suppressing glutamatergic trophic inputs. Consequently, preventing the side effects of NMDA antagonists would be therapeutically useful. Because macroautophagy is involved in the adaptive response to trophic deprivation, the aim of the present study was to investigate the impact of autophagy modulators on the MK801-induced death of immature GABAergic interneurons and to characterize the crosstalk between autophagic and apoptotic mechanisms in this cell type. Ex vivo, using cortical slices from NMRI and Gad67-GFP mice, we show that blockade of the NMDA receptor results in an accumulation of autophagosomes due to the disruption of the autophagic flux. This effect precedes the activation of the mitochondrial apoptotic pathway, and the degeneration of immature GABAergic neurons present in developing cortical layers II-IV and is prevented by 3-MA, an autophagy inhibitor. In contrast, modulators of autophagy (3-MA, rapamycin) do not interfere with the anti-excitotoxic and neuroprotective effect of MK801 observed in deep layers V and VI. In vivo, 3-MA blocks the rapid increase in caspase-3 cleavage induced by the blockade of NMDA receptors and prevents the resulting long-term decrease in Gad67-GFP neurons in layers II-IV. Together, these data suggest that, in the developing cortex, the suppression of glutamatergic inputs through NMDA receptor inhibition results in the impairment of the autophagic flux and the subsequent switch to apoptotic death of immature GABAergic interneurons. The concomitant inhibition of autophagy prevents this pro-apoptotic action of the NMDA blocker and favors the long-term rescue of GABAergic interneurons without interfering with its neuroprotective actions. The use of autophagy modulators in the developing brain would create new opportunities to prevent the side effects of NMDA antagonists used for neuroprotection or anesthesia.
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http://dx.doi.org/10.1016/j.expneurol.2015.02.037DOI Listing
May 2015

Fortuitous detection of a case of unknown haemoglobin Athens-Georgia from atypical HbA1c electropherogram.

Clin Chim Acta 2015 Feb 5;440:6-7. Epub 2014 Nov 5.

Clinical Biology Institute, Rouen University Hospital-Charles Nicolle, Rouen, France. Electronic address:

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http://dx.doi.org/10.1016/j.cca.2014.10.032DOI Listing
February 2015

Calciuria determined by new NM-BAPTA calcium assay is not free from magnesium interference.

Clin Chim Acta 2015 Feb 21;440:113-4. Epub 2014 Nov 21.

Clinical Biology Institute, Rouen University Hospital-Charles Nicolle, Rouen, France.

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http://dx.doi.org/10.1016/j.cca.2014.10.033DOI Listing
February 2015

A 9-year-old child with methemoglobinemia.

Clin Chem 2014 Aug;60(8):1126-7

Laboratoire de biochimie médicale, Institut de biologie clinique, Centre hospitalier universitaire Ch. Nicolle;

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http://dx.doi.org/10.1373/clinchem.2013.218073DOI Listing
August 2014

Liver mass in a young adult.

Lancet 2011 Sep;378(9797):1196

Department of Biochemistry, Centre Hospitalier Universitaire de Rouen, Rouen, France.

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http://dx.doi.org/10.1016/S0140-6736(11)61022-2DOI Listing
September 2011

Is heparin plasma suitable for the determination of B-type natriuretic peptide on the Beckman-Coulter Access 2?

Clin Chem Lab Med 2010 Mar;48(3):399-401

Medical Biochemistry Laboratory, Charles Nicolle Hospital, Rouen-Cedex, France.

Background: The use of heparin as an alternative to EDTA in the production of plasma samples is of particular interest for B-type natriuretic peptide (BNP) measurements. Lithium heparin is now widely used for the determination of biochemical parameters, including cardiac markers. The goal of this study was to determine the feasibility of measuring BNP using heparin plasma instead of EDTA plasma with the Access 2 system (Beckman-Coulter).

Methods: BNP was determined in heparin plasma and EDTA plasma from 24 patients within 1 h of blood collection. Additional measurements were performed with heparin plasma, every hour for the first 4 h, and then 8 h after the collection of blood that was stored at room temperature.

Results: At H(0), the observed BNP concentrations in heparin plasma were much higher (mean values 65% higher) than those in EDTA plasma. Using predetermined thresholds, this difference would lead to 30% discordance between samples in heparin and EDTA. BNP stability decreased over time in heparin plasma: immunoreactivity decreased approximately by 30% during the first 2 h and by 60% after 8 h.

Conclusions: Heparin plasma does not seem to be a suitable alternative to EDTA plasma for measurement of BNP using the Access 2 system, even if measurements are performed immediately after blood sampling.
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http://dx.doi.org/10.1515/CCLM.2010.069DOI Listing
March 2010