Dr. Vaibhav Singh, M.Sc, D.Pharma, PhD - Canadian Center for Behavioural Neuroscience - Postdoctoral fellow

Dr. Vaibhav Singh

M.Sc, D.Pharma, PhD

Canadian Center for Behavioural Neuroscience

Postdoctoral fellow

Alberta | Netherlands

Main Specialties: Biology

Additional Specialties: Neuro-immunology, Multiple Sclerosis, Proteomics-Mass Spectrometry, Immunoproteomics, 2-D Gel electrophoresis, Immunoassays, Protein Biomarkers and translational research, Antibodies, Experimental Autoimmune Encephalitis mouse model, Neurobehavioural testing, Data Analysis

Dr. Vaibhav Singh, M.Sc, D.Pharma, PhD - Canadian Center for Behavioural Neuroscience - Postdoctoral fellow

Dr. Vaibhav Singh

M.Sc, D.Pharma, PhD

Education

Sep 2015
Erasmus University Rotterdam, The Netherlands
PhD
Immuno-Proteomics/Multiple Sclerosis

Publications

8Publications

153Reads

2Profile Views

70PubMed Central Citations

Gray matter-related proteins are associated with childhood-onset multiple sclerosis.

Neurol Neuroimmunol Neuroinflamm 2015 Oct 24;2(5):e155. Epub 2015 Sep 24.

MS Centre ErasMS, Department of Neurology, Erasmus MC, Rotterdam, the Netherlands.

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http://dx.doi.org/10.1212/NXI.0000000000000155DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4582906PMC
October 2015
57 Reads
5 Citations

Identification of Potential Biomarkers in Multiple Sclerosis

Rotterdam : Erasmus Universiteit Rotterdam, 2015

Multiple sclerosis (MScl) is an inflammatory, autoimmune, demyelinating disease of the central nervous system (CNS). The thesis describes the identification of potential MScl biomarker research, with emphasis on distinguishing subtypes, first event of CNS demyelination in children, pathogenic IgG antibodies and pregnancy MScl. Chapter 2 highlights the differences and similarities of CSF proteome profile between primary-progressive (PP) and relapsing- remitting (RR) MScl. Our research indicated that the proteome profiles of these two major subtypes of MScl overlap to a large extent. However, few differences were seen and confirmed by independent techniques for two proteins- Protein jagged-1 (more abundant in RR MScl as compared to PP MScl) and Vitamin D-binding protein (only detected in the RR MScl samples). In Chapter 3, study aimed at the identification of protein markers linked to diagnosis of MScl after the first demyelinating event. Twenty-one proteins differentiated childhood-onset MScl and children presenting with monophasic acquired demyelinating syndromes (ADS). Proteomics analysis revealed that the CSF of childhood onset MScl is associated with the increased abundance of 14 CNS grey matter related proteins as compared to the children presenting with monophasic ADS. Whereas, seven proteins related to the innate immune system were abundant in children with monophasic ADS (such as haptoglobin and CD14). In Chapter 4, we focused on humoral immune responses in CSF of MScl patients. In this study, our main objective was to identify specific proteomic profiles of mutated complementarity determining regions (CDR) of IgG present in MScl patients but absent in controls. We found five CDR shared in three or more patients and not in controls. Interestingly, one CDR with a single mutation was found to be in common in six patients. The result of the study provides leads concerning the question of whether common antigenic stimuli are accountable for the recruitment of intrathecal B cells in MScl. In Chapter 5, we performed a study to monitor the effect of Natalizumab treatment on MScl patients. We addressed its effects on CSF proteome before and after one year of the treatment. This study revealed a number of proteins that were significantly differentially abundant between the before and after treatment groups. Subsequently, three proteins (Ig mu chain C region, haptoglobin and chitinase-3-like protein 1) were validated in an independent sample set and by means of an independent method. In Chapter 6, we performed proteomics approach on alterations of the proteome of urine from MScl patients during third trimester of pregnancy and at first postpartum period. We found that pregnancy related peptides, were significantly elevated in MScl patients compared to controls. When comparing the pregnancy relate changes, we found 43 disease-associated peptides identified with increased or decreased difference ratio in MScl compared to control. Our findings indicated that the peptides that relate strongly to pregnancy are linked with or involved in innate and adaptive immune response either directly or indirectly or were immunosuppressive. Our studies show that we add new information to MScl biomarker field and such biomarkers are of substantial interest with respect to the disease pathology, general understanding, diagnosis and for novel therapeutic targets.

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September 2015
9 Reads

Proteomics urine analysis of pregnant women suffering from multiple sclerosis.

J Proteome Res 2015 May 30;14(5):2065-73. Epub 2015 Mar 30.

Department of Neurology, Erasmus University Medical Center, Dr. Molewaterplein 50, Rotterdam 3015 GE, The Netherlands.

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http://dx.doi.org/10.1021/pr501162wDOI Listing
May 2015
18 Reads
3 Citations
4.250 Impact Factor

Cerebrospinal-fluid-derived immunoglobulin G of different multiple sclerosis patients shares mutated sequences in complementarity determining regions.

Mol Cell Proteomics 2013 Dec 22;12(12):3924-34. Epub 2013 Aug 22.

Department of Neurology, Erasmus University Medical Center, Rotterdam CA 3000, The Netherlands;

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http://dx.doi.org/10.1074/mcp.M113.030346DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3861734PMC
December 2013
41 Reads
5 Citations
6.564 Impact Factor

Effects of natalizumab treatment on the cerebrospinal fluid proteome of multiple sclerosis patients.

J Proteome Res 2013 Mar 4;12(3):1101-7. Epub 2013 Feb 4.

Department of Neurology, Erasmus University Medical Center, Rotterdam, The Netherlands.

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http://dx.doi.org/10.1021/pr3012107DOI Listing
March 2013
15 Reads
10 Citations
4.250 Impact Factor

Proteomics technologies for biomarker discovery in multiple sclerosis.

J Neuroimmunol 2012 Jul 29;248(1-2):40-7. Epub 2011 Nov 29.

Laboratories of Neuro-Oncology/Clinical and Cancer Proteomics, Department of Neurology, Erasmus University Medical Centre, Rotterdam, The Netherlands.

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http://dx.doi.org/10.1016/j.jneuroim.2011.11.004DOI Listing
July 2012
3 Citations
2.470 Impact Factor

Proteomics comparison of cerebrospinal fluid of relapsing remitting and primary progressive multiple sclerosis.

PLoS One 2010 Aug 27;5(8):e12442. Epub 2010 Aug 27.

Laboratory of Neuro-Oncology, Department of Neurology, Erasmus University Medical Centre, Rotterdam, The Netherlands.

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0012442PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2929207PMC
August 2010
18 Reads
14 Citations
3.234 Impact Factor

Proteomics-based study on asthenozoospermia: differential expression of proteasome alpha complex.

Mol Hum Reprod 2010 Jul 18;16(7):452-62. Epub 2010 Mar 18.

Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.

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http://dx.doi.org/10.1093/molehr/gaq009DOI Listing
July 2010
4 Reads
30 Citations
3.750 Impact Factor

Top co-authors

Theo M Luider
Theo M Luider

Erasmus University Medical Center

6
Rogier Q Hintzen
Rogier Q Hintzen

Erasmus University Medical Centre

6
Marcel P Stoop
Marcel P Stoop

Erasmus University Medical Centre

6
Christoph Stingl
Christoph Stingl

Erasmus University Medical Center

5
Lennard J Dekker
Lennard J Dekker

Erasmus University Medical Center

2
Rinze F Neuteboom
Rinze F Neuteboom

Erasmus University Medical Centre

2
Duvvuri Butchi Kameshwari
Duvvuri Butchi Kameshwari

Centre for Cellular and Molecular Biology

1
Peter C Burgers
Peter C Burgers

Erasmus University Medical Centre

1
Archana Bharadwaj Siva
Archana Bharadwaj Siva

CSIR-Centre for Cellular and Molecular BiologyHyderabad 500007

1