Publications by authors named "V Beena"

55 Publications

Molecular and pathological screening of canine distemper virus in Asiatic lions, tigers, leopards, snow leopards, clouded leopards, leopard cats, jungle cats, civet cats, fishing cat, and jaguar of different states, India.

Infect Genet Evol 2022 03 18;98:105211. Epub 2022 Jan 18.

ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly-243122, Uttar Pradesh, India.. Electronic address:

The present investigation was conducted to rule out canine distemper (CD) diseases in Indian wild felids (Asiatic lions, tigers, leopards, snow leopards, clouded leopards, leopard cats, jungle cats, civet cats, fishing cat, and jaguar). The collected samples were screened for CD virus (CDV) by histopathology (HP), immunohistochemistry (IHC) and reverse transcriptase-polymerase chain reaction (RT-PCR) targeting H gene and N gene. The HP and IHC of suspected samples portrayed that 22 [11 leopards, 6 lions, 3 tigers, 1 snow leopard and 1 civet cat] out of 129 (17.05%) wild felids were positive for CD. The major pathological consequences were observed in spleen, lung, kidney and brain. The syncytia and intranuclear as well as intracytoplasmic eosinophilic inclusion bodies were seen in CDV infected cells. Although the histopathological lesions in spleen were more specific and consistent, however, the severe demyelinated leukoencephalitis (usually expected in CD infected dog) was not observed in the brain of any Indian wild felids. Conversely, the CDV antigen has been portrayed via IHC in pancreatic islets of Langerhans of tiger species for the first time in this study. Moreover, the concurrent CD and babesiosis has also been observed in a lioness without a usual coffee-coloured urine. The N gene and H gene of CDV isolates were amplified, sequenced and subsequently constructed the phylogenetic tree. The phylogenetic analysis of H gene revealed that the CDV isolates from Indian lion formed separate clade with CDV isolates from Indian dog and Indian palm civet cat. Furthermore, two CDV isolates from Indian tigers formed clade with Onderstepoort vaccine strain and CDV isolates from dogs of Uttar Pradesh, USA and UK. Evidently, CDV is circulating in Indian wild felids and causing diseases in them.
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http://dx.doi.org/10.1016/j.meegid.2022.105211DOI Listing
March 2022

Shear Stress Alterations Activate BMP4/pSMAD5 Signaling and Induce Endothelial Mesenchymal Transition in Varicose Veins.

Cells 2021 12 17;10(12). Epub 2021 Dec 17.

Cardiovascular Diseases and Diabetes Biology, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram 695014, India.

Chronic venous diseases, including varicose veins, are characterized by hemodynamic disturbances due to valve defects, venous insufficiency, and orthostatism. Veins are physiologically low shear stress systems, and how altered hemodynamics drives focal endothelial dysfunction and causes venous remodeling is unknown. Here we demonstrate the occurrence of endothelial to mesenchymal transition (EndMT) in human varicose veins. Moreover, the BMP4-pSMAD5 pathway was robustly upregulated in varicose veins. In vitro flow-based assays using human vein, endothelial cells cultured in microfluidic chambers show that even minimal disturbances in shear stress as may occur in early stages of venous insufficiency induce BMP4-pSMAD5-based phenotype switching. Furthermore, low shear stress at uniform laminar pattern does not induce EndMT in venous endothelial cells. Targeting the BMP4-pSMAD5 pathway with small molecule inhibitor LDN193189 reduced SNAI1/2 expression in venous endothelial cells exposed to disturbed flow. TGFβ inhibitor SB505124 was less efficient in inhibiting EndMT in venous endothelial cells exposed to disturbed flow. We conclude that disturbed shear stress, even in the absence of any oscillatory flow, induces EndMT in varicose veins activation of BMP4/pSMAD5-SNAI1/2 signaling. The present findings serve as a rationale for the possible use of small molecular mechanotherapeutics in the management of varicose veins.
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http://dx.doi.org/10.3390/cells10123563DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8700678PMC
December 2021

Mucosal keratocyst of buccal mucosa: A rare entity.

J Oral Maxillofac Pathol 2020 Sep-Dec;24(3):589. Epub 2021 Jan 9.

Department of Oral Pathology and Microbiology, Government Dental College, Thrissur, Kerala, India.

The odontogenic keratocyst (OKC) is a developmental odontogenic cyst that is important due to its specific histopathologic features and clinical behavior. It is well known that the OKC arises from cell rests of the dental lamina. This cyst is important as it shows a different growth mechanism and biologic behavior from other common odontogenic cysts. In rare cases, OKCs occur in sites other than intraosseous sites. The most common location of peripheral OKC is the gingiva, but mucosal, epidermal and even intramuscular sites have also been described. The origin of peripheral OKCs is still under controversy. We, hereby, add a case of peripheral OKC located in the soft tissue of the buccal mucosa to the scarce literature consisting of only a few case reports and small case series of peripheral odontogenic cysts.
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http://dx.doi.org/10.4103/jomfp.JOMFP_54_20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8083401PMC
January 2021

Emerging horizon for bat borne viral zoonoses.

Authors:
V Beena G Saikumar

Virusdisease 2019 Sep 26;30(3):321-328. Epub 2019 Oct 26.

2ICAR-Indian Veterinary Research Institute, Bareilly, UP 243122 India.

Bats are the only flying placental mammals that constitute the second largest order of mammals and present all around the world except in Arctic, Antarctica and a few oceanic islands. Sixty percent of emerging infectious diseases originating from animals are zoonotic and more than two-thirds of them originate in wildlife. Bats were evolved as a super-mammal for harboring many of the newly identified deadly diseases without any signs and lesions. Their unique ability to fly, particular diet, roosting behavior, long life span, ability to echolocate and critical susceptibility to pathogens make them suitable host to harbor numerous zoonotic pathogens like virus, bacteria and parasite. Many factors are responsible for the emergence of bat borne zoonoses but the most precipitating factor is human intrusions. Deforestation declined the natural habitat and forced the bats and other wild life to move out of their niche. These stressed bats, having lost foraging and behavioral pattern invade in proximity of human habitation. Either directly or indirectly they transmit the viruses to humans and animals. Development of fast detection modern techniques for viruses from the diseased and environmental samples and the lessons learned in the past helped in preventing the severity during the latest outbreaks.
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http://dx.doi.org/10.1007/s13337-019-00548-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6864002PMC
September 2019

strains from Western Ghats of India possess nematocidal property against larvae of goats.

Heliyon 2019 Oct 1;5(10):e02724. Epub 2019 Nov 1.

Department of Veterinary Physiology, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Mannuthy, Thrissur, Kerala, India.

Nematocidal properties of spore crystal mixtures of six (Bt) strains (KAU 49, 50, 52, 61, 99 and 424) collected from Western Ghats, a biodiversity hot spot of India, were analysed against larvae isolated from goats. One dose nematocidal assay dose response to lyophilised spore-crystal mixtures (SCM) of the six Bt strains were determined by adding 200 μg/mL of each SCMs to culture plate wells containing aqueous suspension of larvae. Out of the strains screened, KAU 50 and 424 were found to possess nematocidal properties. Maximum nematocidal properties were exhibited 7 days post-inoculation of the lyophilised SCMs. The 50 per cent lethal concentrations deduced by log probit analysis for KAU 50 was found to be 130.59 μg/mL, whereas that of KAU 424 was found to be 144.536 μg/mL at 95 per cent confidence level. This is the first report on the nematocidal propery of Bt strains against larvae isolated from goats. Further studies are needed for identification and characterisation of the toxin.
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http://dx.doi.org/10.1016/j.heliyon.2019.e02724DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6838916PMC
October 2019
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