Publications by authors named "Uk-Kyu Kim"

53 Publications

Sequential treatment from mandibulectomy to reconstruction on mandibular oral cancer - Case review II: mandibular anterior and the floor of the mouth lesion of basaloid squamous cell carcinoma and clear cell odontogenic carcinoma.

J Korean Assoc Oral Maxillofac Surg 2021 Jun;47(3):216-223

Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, Yangsan, Korea.

Preoperative patient analysis for oral cancer involves multiple considerations that are based on multiple factors; these include TNM stages, histopathologic findings, and adjacent anatomical structures. Once the decision is made to excise the lesion, the margin of dissection and its extent should be considered along with the best form of reconstruction and airway management. Treatment methods include surgical resection, radiotherapy, and chemotherapy. Although the combined method of treatment is controversial, surgical resection is considered predominantly, and immediate reconstruction after surgical resection follows. The choice of treatment is dictated by the anticipated functional and esthetic results of treatment and also by the availability of a surgeon with the required expertise. Segmental mandibulectomy with primary reconstruction has been shown to have advantages in both functional and esthetic results. A 52-year-old male patient with basaloid squamous cell carcinoma of the floor of the mouth, and the anterior portion of the mandible was treated with surgical procedures that included segmental mandibulectomy with both supraomohyoid neck dissection (SOHND) at Levels I-III and mandible reconstruction with a left fibula free flap. A 55-year-old male patient with clear cell odontogenic carcinoma of the oral cavity underwent segmental mandibulectomy with both SOHND at Levels I-III and mandible reconstruction with a left fibula free flap. The purpose of this study was to review the anatomic and functional results of patients after immediate reconstruction with a fibula free flap following resection of carcinoma in the anterior portion of the mandible and floor of the mouth.
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http://dx.doi.org/10.5125/jkaoms.2021.47.3.216DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8249190PMC
June 2021

Comparison of time and cost between conventional surgical planning and virtual surgical planning in orthognathic surgery in Korea.

Maxillofac Plast Reconstr Surg 2021 Jun 21;43(1):18. Epub 2021 Jun 21.

Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, Busandaehak-ro, Mulgeum-eup, Yangsan, 50612, Republic of Korea.

Background: The purpose of this study was to measure the time of the conventional surgical planning (CSP) and virtual surgical planning (VSP) in orthognathic surgery and to compare them in terms of cost.

Material And Method: This is a retrospective study of the patients who underwent orthognathic surgery at the OOOOO University Dental Hospital from December 2017 to August 2018. All the patients were analyzed through both CSP and VSP, and all the surgical stents were fabricated through manual and 3-dimensional (3D) printing. The predictor variables were the planning method (CSP vs. VSP) and the surgery type (group I: Le Fort I osteotomy+bilateral sagittal split osteotomy [LFI+BSSO] or group II: only bilateral sagittal split osteotomy [BSSO]), and the outcomes were the time and cost. The results were analyzed using paired t test.

Results: Thirty patients (12 females, 18 males) met the inclusion criteria, and 17 patients were excluded from the study due to missing or incomplete data. There were 20 group I patients (LFI+BSSO regardless of genioplasty) and 10 group II patients (BSSO regardless of genioplasty). The average time of CSP for group I was 385±7.8 min, and that for group II was 195±8.33 min. The time reduction rate of VSP compared with CSP was 62.8% in group I and 41.5% in group II. On the other hand, there was no statistically significant cost reduction.

Conclusions: The time investment in VSP in this study was significantly smaller than that in CSP, and the difference was greater in group I than in group II.
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http://dx.doi.org/10.1186/s40902-021-00305-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8217346PMC
June 2021

The effect of autogenous tooth bone graft material without organic matter and type I collagen treatment on bone regeneration.

Maxillofac Plast Reconstr Surg 2021 Jun 18;43(1):17. Epub 2021 Jun 18.

Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, Yangsan, 50612, Republic of Korea.

Objectives: The aim of this study is to examine the effect of particulate autogenous tooth graft removed with organic matter and type I collagen addition on bone regeneration and to validate the possibility of useful allograft material for jaw defects.

Material And Methods: Autogenous tooth bone maker (Korean Dental Solution® KOREA) made particulate autogenous tooth not including organic matter. We used to the developed tooth grafts for experiment. Cell adhesion test with hemacytometer and energy dispersive X-ray spectroscopy (Supra40 VP®, Carl Zeiss, Germany) analysis about the particulate autogenous tooth and type I collagen were performed. Rabbits were divided into three groups: bone graft with organic matter (OM) removing particulate autogenous tooth group, bone graft with OM removing particulate autogenous tooth and type I collagen group, and a control group. Bone grafting was performed in rabbit's calvaria. The rabbits were sacrificed at different interval at 1, 2, 4, and 6 weeks after bone grafting for the histopathologic observation and observed the effect of bone regeneration by SEM, H-E & Masson stains, osteocalcin IHC staining.

Result: In vitro cytopathological study showed affinity for cells, cell attachment pattern, and cell proliferation in the order of control group, OM-removed and collagen-treated group, OM-removed particulate autogenous tooth group. The results of the degree of mineralization were opposite to those of the previous cell experimental results, and the OM-removed group, OM-removed group and collagen-treated group were relatively higher than the control group. Histopathologic analysis showed that vascularization and neonatal bone formation were higher in particulate autogenous tooth group with removing OM and with addition of collagen than control group and group of OM removed only. Immunohistochemical analysis showed that osteocalcin (OSC) expression was not observed in the control group, but at 4 weeks groups, OSC expression was observed the OM removed and OM-removed-collagen-treated particulate autogenous tooth, and the degree of expression was somewhat stronger in group of the OM removed and collagen additionally treated particulate autogenous tooth.

Conclusion: Particles that do not contain organic matter, the saint tooth, was responsible for sufficient bone graft material through the role of space maintenance and bone conduction, and further improved bone formation ability through additional collagen treatment. Therefore, research on various extracellular substrates and autologous bone grafting materials is necessary, and through this, it is possible to lay the foundation for a new type of autologous bone grafting material with excellent academic and technical utility.
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http://dx.doi.org/10.1186/s40902-021-00302-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212298PMC
June 2021

Sequential treatment from mandibulectomy to reconstruction on mandibular oral cancer - Case review I: mandibular ramus and angle lesion of primary intraosseous squamous cell carcinoma.

J Korean Assoc Oral Maxillofac Surg 2021 Apr;47(2):120-127

Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, Yangsan, Korea.

Primary intraosseous squamous cell carcinoma (PIOSCC) is very rare type of squamous cell carcinoma (SCC) that occurs within the jaw and arises from remnants of odontogenic epithelium with no connection to the oral mucosa. This study reports two cases of PIOSCC of the mandible. Reported in this article are two cases of PIOSCC of the mandible that were treated with resection and reconstruction using a fibular free flap. The first case was a 36-year-old male patient who complained of right mandibular pain. Computed tomography (CT) and panoramic radiograph revealed a large radiolucency in the mandibular ramus area. At first, an odontogenic keratocyst was tentatively diagnosed, and an excision procedure was carried out at another clinic. A final biopsy after cyst enucleation revealed well-differentiated SCC, so we proceeded with segmental mandibulectomy and reconstruction using a fibular free flap. The second case was a 48-year-old male patient with left mandibular pain. CT and panoramic radiograph revealed irregular radiolucency in the mandibular angle area near tooth #38. At first, osteomyelitis was tentatively diagnosed, and a curettage was carried out. A later biopsy revealed well-differentiated SCC, so segmental mandibulectomy and reconstruction with a fibular free flap were secondarily performed. Our two cases have had no recurrence. The facial appearance of both patients is satisfactory, and the neo-mandibular body created using a fibular bone transfer displays adequate bony volume.
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http://dx.doi.org/10.5125/jkaoms.2021.47.2.120DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8084740PMC
April 2021

Gold nanoparticles conjugated with programmed death-ligand 1 antibodies induce apoptosis of SCC-25 oral squamous cell carcinoma cells via programmed death-ligand 1/signal transducer and transcription 3 pathway.

Arch Oral Biol 2021 May 16;125:105085. Epub 2021 Feb 16.

Department of Oral Anatomy, School of Dentistry, Pusan National University, Yangsan 50612, Republic of Korea. Electronic address:

Objective: Objective of this study is to test the anti-cancer effect of the gold nanoparticles conjugated with programmed death-ligand 1 (PD-L1) specific antibodies (PDL1-GNP), on oral squamous cell carcinoma.

Design: To test the effect of PDL1-GNP on oral squamous cell carcinoma, SCC-25 cells, a type of human oral squamous cell carcinoma which were isolated from human tongue, and HaCaT human keratinocytes as normal cell control, were used. Cell viability was tested by the water-soluble tetrazolium-1 and live/dead assays, while apoptotic cell death of SCC-25 cells were monitored by immunofluorescent staining and flow cytometry. The molecular changes during PDL1-GNP-mediated apoptosis were analyzed using Western blot analysis.

Results: PDL1-GNP treatment effectively decreased the growth of SCC-25 cells but not HaCaT cells. The results of the confocal microscopic assay showed that PDL1-GNP specifically bound to the SCC-25 cell membrane. Furthermore, the results of the live/dead, cytochrome c release assays and flow cytometry indicated PDL1-GNP-mediated apoptotic cell death of SCC-25 cells. PDL1-GNP-treated SCC-25 cells showed a phenotype with increased apoptotic proteins, including cleaved form of caspase-3, caspase-9, and poly (ADP-ribose) polymerase 1 (PARP1). PDL1-GNP treatment also effectively decreased B-cell lymphoma 2 (Bcl-2) and PD-L1 protein expression. Phosphorylation of signal transducer and transcription 3 (STAT3) was significantly increased after PDL1-GNP treatment on SCC-25 cells.

Conclusions: PDL1-GNP treatment induced SCC-25 cell apoptosis possibly by inhibiting the function of the PD-L1 protein, since PD-L1 blocks STAT3 phosphorylation, which promotes apoptotic cell death.
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http://dx.doi.org/10.1016/j.archoralbio.2021.105085DOI Listing
May 2021

Non-thermal plasma application enhances the recovery of transected sciatic nerves in rats.

Exp Biol Med (Maywood) 2021 Jun 2;246(11):1287-1296. Epub 2021 Mar 2.

Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, Gyeongnam 50612, Republic of Korea.

This experimental research aimed to investigate the effects of non-thermal plasma on nerve regeneration after transected nerve damage using the sciatic nerve in Wistar albino (A) rats. The experiments were performed on 27 Wistar A rats. The rats underwent surgery for right sciatic nerve exposure and were divided into three groups (each group,  = 9) according to sciatic nerve transected injury (SNTI) and non-thermal plasma application: a non-nerve damage (non-ND) group, a only nerve damage without non-thermal plasma application (ND) group, and a nerve damage with non-thermal plasma application (ND + NTP) group. Subsequent to SNTI and immediate suture, non-thermal plasma was administered three times per week for eight weeks. Evaluation for functional recovery was performed using the static sciatic index measured over the full treatment period of eight weeks. The sciatic nerve specimens were obtained after euthanasia and third day from the last non-thermal plasma application. The sciatic nerve tissues were subjected to histological analysis. Behavior analysis presented that the ND + NTP group showed improved static sciatic index compared with the nerve damage group. Histopathological findings demonstrated that the ND + NTP group had more dense Schwann cells and well-established continuity of nerve fibers, greater than the nerve damage group. Immunohistochemistry showed that the ND + NTP group had increased levels of markers for microtubule-associated protein 2 (MAP2), tau, S100 calcium-binding protein B, and neurofilament-200 and regulated the overexpression of CD68 and MAP2. These results indicated that non-thermal plasma enhanced the motor function and restored the neuronal structure by accelerating myelination and axonal regeneration. Additionally, non-thermal plasma was confirmed to have a positive effect on the recovery of SNTI in rats.
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http://dx.doi.org/10.1177/1535370221996655DOI Listing
June 2021

Sports-Related Oral and Maxillofacial Injuries: A 5-Year Retrospective Study, Pusan National University Dental Hospital.

J Oral Maxillofac Surg 2021 Jan 5;79(1):203.e1-203.e8. Epub 2020 Aug 5.

Professor, Department of Oral Maxillofacial Surgery, School of Dentistry, Pusan National University, Yangsan, Gyeongnam, Republic of Korea. Electronic address:

Purpose: The incidence of sports trauma is gradually increasing, and its importance is therefore also increasing. The present study was conducted to analyze the types, proportions, and tendencies of sports trauma associated with the oral and maxillofacial regions.

Patients And Methods: Patients who visited the Emergency Department of Pusan National University Dental Hospital between 2014 and 2018 for more than 5 years were surveyed through retrospective epidemiologic investigations. Type of sports was classified according to American Academy of Pediatrics classification. For statistical analysis, age and gender distribution, cause of trauma, and annual trends data were collected.

Results: Of the 517 patients, most of the patients were teenagers (27.9%), followed by those younger than 10 years (23.2%) (χ = 22.897; P = .002), and noncontact sports, which is cycling, was the most common (43.5%) cause for trauma in both adult and children groups (χ = 91.824; P < .001). The most common sports associated with contact sports, limited-contact sports, and noncontact sports were football (47.7%), baseball (50.0%), and cycling (74.8%), respectively. The causes for trauma injury were as follows: contact sports, other person's body (53.4%); limited-contact sports, other objects (60.9%); and noncontact sports, slip down (77.4%) (χ = 298.901; P < .001). The prevalence and incidence of sports injuries increase every year, and the proportion of injured patients in May was the highest (χ = 52.360; P = .181). The high percentage of traumatic sports demonstrated statistically significant trends (χ = 43.073; P = .002). Kickboard injuries showed a rapid increase recently (P for linear trend = .045), whereas other sports showed no significant trends.

Conclusions: Considering the increasing incidence of sports-related injuries, oral and maxillofacial surgeons should be more concerned with maxillofacial trauma during sports.
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http://dx.doi.org/10.1016/j.joms.2020.07.218DOI Listing
January 2021

Importance in the occurrence rate of shortest buccal bone marrow distance (<1 mm) for sagittal split ramus osteotomy.

J Formos Med Assoc 2021 Jan 1;120(1 Pt 3):697-704. Epub 2020 Aug 1.

School of Dentistry and Graduate Program of Dental Science, College of Dental Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan; Department of Orthodontics, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan. Electronic address:

Background/purpose: The neurosensory disturbance is a common complication following sagittal split ramus osteotomy (SSRO) whereas the shortest buccal bone marrow (SBM) is an important risk factor. The present study aimed to investigate the relationship between the occurrence rates of SBM among three skeletal patterns.

Methods: The cone-beam computed tomography (CBCT) images of 90 participants were divided into skeletal Class I, II, and III. There were six horizontal planes separated apart by a 2 mm interval; it started with plane 0 (original intact mandibular canal) to plane 5 which was 10 mm below. The data of SBM were divided into two groups (SBM ≥ 1 mm and SBM < 1 mm). With an SBM value < 1 mm, we defined a high occurrence rate of postoperative neurosensory abnormality or unfavorable split.

Results: The Class III patients had the smallest SBM value (1.31-1.75 mm) whereas the Class II patients had the largest SBM value (1.57-2.09 mm). For the Class III patients, the highest and lowest occurrence rates of SBM were 56.5% and 43.5% respectively. For the Class II patients, the highest and lowest occurrence rates of SBM were 37.1% and 17.7% respectively. The patients with Class III malocclusion had higher occurrence rates of SBM than the patients with Class II malocclusion.

Conclusion: Class III had a significantly higher occurrence of probability (SBM < 1 mm) than Class II. Therefore, patients with Class III were more likely to experience postoperative neurosensory abnormalities and unfavorable split than patients with Class II.
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http://dx.doi.org/10.1016/j.jfma.2020.07.030DOI Listing
January 2021

Non-thermal plasma accelerates the healing process of peripheral nerve crush injury in rats.

Int J Med Sci 2020 27;17(8):1112-1120. Epub 2020 Apr 27.

Department of Oral & Maxillofacial Surgery, School of Dentistry, Pusan National University.

The objective of this study was to evaluate the effect of non-thermal plasma (NTP) on the healing process of peripheral nerve crush injuries, which can occur during dental implant procedures. For this, a rat model of sciatic nerve crush injury (SNCI) was adopted. The rats were divided into three groups: non-nerve damage (non-ND), nerve damage (ND), and ND+NTP group. To evaluate the sciatic nerve (SN) function, the static sciatic index was calculated, and the muscle and SN tissues were subjected to a histologic analysis. The results showed that NTP effectively accelerated the healing process of SNCI in rats. In contrast to the ND group, which showed approximately 60% recovery in the SN function, the NTP-treated rats showed complete recovery. Histologically, the NTP treatments not only accelerated the muscle healing, but also reduced the edema-like phenotype of the damaged SN tissues. In the ND group, the SN tissues had an accumulation of CD68-positive macrophages, partially destroyed axonal fibers and myelinated Schwann cells. Conversely, in the ND+NTP group, the macrophage accumulation was reduced and an overall regeneration of the damaged axon fibers and the myelin sheath was accomplished. The results of this study indicate that NTP can be used for healing of injured peripheral nerves.
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http://dx.doi.org/10.7150/ijms.44041DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211154PMC
March 2021

Complications associated with specific characteristics of supernumerary teeth.

Oral Surg Oral Med Oral Pathol Oral Radiol 2020 Aug 5;130(2):150-155. Epub 2020 May 5.

Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, Yangsan, Korea; Dental and Life Science Institute & Dental Research Institute, School of Dentistry, Pusan National University, Busan, Yangsan, Korea. Electronic address:

Objectives: The purpose of this study was to identify the characteristics of supernumerary teeth, analyze the associated complications, and to present new clinical knowledge on surgical interventions for supernumerary teeth.

Study Design: This retrospective cohort study was based on the medical records and radiographic records of patients who underwent surgical extraction of supernumerary teeth. The relationships among the patient's age, gender, anatomic features of supernumerary teeth, and presence and type of complications (i.e., spacing, rotation, delayed eruption of the adjacent tooth, cyst formation.) were investigated. The groups were compared by using the Mann-Whitney U test, the Kolmogorov-Smirnov test, and multiple logistic regression analysis (P < .05).

Results: The study population consisted of 705 participants who underwent extraction for 1036 supernumerary teeth. The mean age of the participants was 11.5 years, and 73.5% of the participants were males. The complication rate was 55.6%. Variables associated with an increased risk of complications were the patient's age, dentition, tuberculate shape, and horizontal direction of eruption (P < .05).

Conclusions: An increase in the patient's age or abnormalities in the shape and direction of eruption of supernumerary teeth was associated with complications. These parameters should be considered while formulating the treatment plan.
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http://dx.doi.org/10.1016/j.oooo.2020.03.002DOI Listing
August 2020

Versatility of the pedicled buccal fat pad flap for the management of oroantral fistula: a retrospective study of 25 cases.

Maxillofac Plast Reconstr Surg 2019 Dec 25;41(1):50. Epub 2019 Nov 25.

1Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, Yangsan, Republic of Korea.

Purpose: Maxillary bone grafts and implantations have increased over recent years despite a lack of maxillary bone quality and quantity. The number of patients referred for oroantral fistula (OAF) due to implant or bone graft failure has increased, and in patients with an oroantral fistula, the pedicled buccal fat pad is viewed as a robust, reliable option. This study was conducted to document the usefulness of buccal fat pad grafts for oroantral fistula closure.

Materials And Methods: We retrospectively studied 25 patients with OAF treated with a buccal fat pad graft from 2015 to 2018. Sex, age, OAF location, cause, duration, presence of systemic disease, smoking, previous dental surgery, and side effects were investigated.

Results: A total of 25 patients were studied. Mean patient age was 54.8 years, and the male to female ratio was 19:6. Causes of oroantral fistula were cyst enucleation, tumor resection, implant removal, bone graft failure, and extraction. Excellent results were obtained in 23 (92%) of the 25 patients. In the other two patients that both smoked, a small fistula was observed during follow-up. No recurrence of oroantral fistula was observed after 2 months to 1 year of follow-up.

Conclusions: The incidence of oroantral fistula is increasing due to implant and bone graft failures. Oroantral fistula closure using a pedicled buccal fat pad was found to have a high success rate.
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http://dx.doi.org/10.1186/s40902-019-0229-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6877680PMC
December 2019

A retrospective analysis of risk factors of oromaxillofacial infection in patients presenting to a hospital emergency ward.

Maxillofac Plast Reconstr Surg 2019 Dec 22;41(1):49. Epub 2019 Nov 22.

1Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, Beomeo, Mulgeum, Yangsan, 50612 Republic of Korea.

Background: The purpose of this study was to review the clinical features of oromaxillofacial infections in patients presenting to a hospital emergency ward, to identify the key factors affecting the requirement for hospitalization, and the potential risk factors predisposing to a prolonged length of hospital stay.

Methods: A retrospective medical record review of the 598 patients treated for oromaxillofacial infection from 2013 to 2017 at the oral and maxillofacial surgery department, Yangsan Pusan National University Hospital, was conducted. The following information was collected from each patient: sex, age, past medical history, site of infection, etiology, admission or outpatient care, level of C-reactive protein (mg/dL), fascial spaces involved, treatment method, and duration of hospitalization. Chi-squared tests were used to identify risk factors, which were further analyzed using multivariable logistic regression.

Results: A total of 606 patients were eligible for inclusion in the study, of which eight were excluded due to having incomplete charts; thus, 598 patients were included: 55% were male, mean patient age was 47.1 ± 19.9 years, and 12.9% of patients were diabetic. Furthermore, 71.2% of patients had infection originating in the mandible; the most common tooth of origin was lower posterior, and 29.8% of patients were hospitalized. Risk factors for hospital admission were elderly patients with concurrent disease, elevated C-reactive protein level, and multiple-space infection in the oromaxillofacial area. The duration of hospitalization was correlated with both diabetes and age.

Conclusions: The requirement for hospital admission is determined by the severity of the infection; even severe infections, once treated with appropriate surgery, have no relation to the length of hospital stay. The important risk factors for increased duration of hospitalization are diabetes mellitus and older age. The understanding of risk factors associated with a prolonged hospital stay during the treatment of oromaxillofacial infection will aid in treatment planning as well as highlight the importance of adequate diabetes control in patients at risk of such infection.
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http://dx.doi.org/10.1186/s40902-019-0238-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6872703PMC
December 2019

Comparison of time and cost between conventional surgical planning and virtual surgical planning in orthognathic surgery in Korea.

Maxillofac Plast Reconstr Surg 2019 Dec 12;41(1):35. Epub 2019 Sep 12.

1Department of Oral and Maxillofacial surgery School of dentistry, Pusan National University, Busandaehak-ro, Mulgeum-eup, Yangsan, 50612 Republic of Korea.

Background: The purpose of this study was to measure the time of the conventional surgical planning (CSP) and virtual surgical planning (VSP) in orthognathic surgery and to compare them in terms of cost.

Material And Method: This is a retrospective study of the patients who underwent orthognathic surgery at the Pusan National University Dental Hospital from December 2017 to August 2018. All the patients were analyzed through both CSP and VSP, and all the surgical stents were fabricated through manual and three-dimensional (3D) printing. The predictor variables were the planning method (CSP vs. VSP) and the surgery type (group I: Le Fort I osteotomy + bilateral sagittal split osteotomy [LFI+BSSO] or group II: only bilateral sagittal split osteotomy [BSSO]), and the outcomes were the time and cost. The results were analyzed using the paired test.

Results: Thirty patients (12 females, 18 males) met the inclusion criteria, and 17 patients were excluded from the study due to missing or incomplete data. There were 20 group I patients (LFI+BSSO regardless of genioplasty) and 10 group II patients (BSSO regardless of genioplasty). The average time of CSP for group I was 385 ± 7.8 min, and that for group II was 195 ± 8.33 min. The time reduction rate of VSP compared with CSP was 62.8% in group I and 41.5% in group II. On the other hand, there was no statistically significant cost reduction.

Conclusions: The time investment in VSP in this study was significantly smaller than that in CSP, and the difference was greater in group I than in group II.
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http://dx.doi.org/10.1186/s40902-019-0220-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6739435PMC
December 2019

Langerhans cell histiocytosis of the mandible: two case reports and literature review.

J Korean Assoc Oral Maxillofac Surg 2019 Jun 28;45(3):167-172. Epub 2019 Jun 28.

Department of Translational Dental Science, School of Dentistry, Pusan National University, Yangsan, Korea.

Langerhans cell histiocytosis (LCH) is a rare disorder characterized by the proliferation of dendritic cells resulting in local or systemic symptoms. The clinical symptoms of patients with Langerhans cell histiocytosis depend on the site and the degree of involvement. This article describes two case histories of unifocal bony Langerhans cell histiocytosis with mandibular involvement and further discusses the appropriate management of such via a review of the literature.
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http://dx.doi.org/10.5125/jkaoms.2019.45.3.167DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6620304PMC
June 2019

Induction of Melanoma Cell-Selective Apoptosis Using Anti-HER2 Antibody-Conjugated Gold Nanoparticles.

Yonsei Med J 2019 Jun;60(6):509-516

Department of Oral Anatomy, School of Dentistry, Pusan National University, Yangsan, Korea.

Purpose: This study was conducted to verify the induction and mechanism of selective apoptosis in G361 melanoma cells using anti-HER2 antibody-conjugated gold nanoparticles (GNP-HER2).

Materials And Methods: Following GNP-HER2 treatment of G361 cells, cell cycle arrest and apoptosis were measured by WST-1 assay, Hemacolor staining, Hoechst staining, immunofluorescence staining, fluorescence-activated cell sorting analysis, and Western blotting.

Results: G361 cells treated with GNP-HER2 showed condensation of nuclei, which is an apoptotic phenomenon, and translocation of apoptosis-inducing factor and cytochrome c from mitochondria into the nucleus and cytoplasm, respectively. Increases in BAX in cells undergoing apoptosis, activation of caspase-3 and -9, and fragmentation of poly (ADP-ribose) polymerase and DNA fragmentation factor 45 (inhibitor of caspase-activated DNase) were observed upon GNP-HER2 treatment. Following GNP-HER2 treatment, an increase of cells in sub-G1 phase, which is a signal of cell apoptosis, was observed. This resulted in the down-regulation of cyclin A, cyclin D1, cyclin E, cdk2, cdk4, and cdc2 and the up-regulation of p21. Thus, GNP-HER2 treatment was confirmed to induce the cessation of cell cycle progression. Also, decreases in phospho-focal adhesion kinase and phospho-human epidermal growth factor receptor, which activate cellular focal adhesion, and decreases in phospho-paxillin, which stimulates the disassembly of filamentous actin, were observed. Reduced cell adhesion and disassembly of the intracellular structure indicated cell deactivation.

Conclusion: GNP-HER2 can selectively kill G361 melanoma cells without affecting normal cells. The mechanism of G361 cell death upon treatment with GNP-HER2 was apoptosis accompanied by activation of caspases.
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http://dx.doi.org/10.3349/ymj.2019.60.6.509DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6536400PMC
June 2019

Misdiagnosis of ameloblastoma in a patient with clear cell odontogenic carcinoma: a case report.

J Korean Assoc Oral Maxillofac Surg 2019 Apr 29;45(2):116-120. Epub 2019 Apr 29.

Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, Yangsan, Korea.

Clear cell odontogenic carcinoma (CCOC), a rare tumor in the head and neck region, displays comparable properties with other tumors clinically and pathologically. In consequence, an incorrect diagnosis may be established. A 51-year-old male patient who was admitted to the Department of Oral and Maxillofacial Surgery at Pusan National University Dental Hospital was initially diagnosed with ameloblastoma via incisional biopsy. However, the excised mass of the patient was observed to manifest histopathological characteristics of ameloblastic carcinoma. The lesion was ultimately diagnosed as clear cell odontogenic carcinoma by the Department of Oral Pathology of Pusan National Dental University. Therefore, segmental mandibulectomy and bilateral neck dissection were performed, followed by reconstruction with fibula free flap and reconstruction plate. Concomitant chemotherapy radiotherapy was not necessary. The patient has been followed up, and no recurrence has occurred 6 months after surgery.
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http://dx.doi.org/10.5125/jkaoms.2019.45.2.116DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6502756PMC
April 2019

Complications Following Orthognathic Surgery for Patients With Cleft Lip/Palate.

J Craniofac Surg 2019 Sep;30(6):1815-1819

Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, Yangsan, South Korea.

Introduction: Cleft lip/palate is a facial anomaly caused by an abnormal developmental process. It is also the most common congenital anomaly. Orthognathic surgery is required in 25% of patients with cleft lip and palate for the correction of dentofacial deformity. There are various complications that can occur after orthognathic surgery. Complications that can occur during surgery include bleeding, improper fracture, and injuries to the inferior alveolar nerve (IAN) and lingual nerve. Meanwhile, postoperative complications include hemorrhage, edema, pain, infection, and delayed union or nonunion. This study retrospectively examines the complications that occurred after the orthognathic surgery in cleft lip/palate patients at Pusan National University Dental Hospital.

Patients And Methods: From June 1, 2008 to July 31, 2017, we selected 17 patients who underwent orthognathic surgery for cleft lip/palate at the Department of Oral and Maxillofacial Surgery, Pusan National University Dental Hospital. The patients were treated at different hospitals for all operations related to cleft lip/palate.

Result: Intraoperative complications include hemorrhage, inadequate fracture, injury to the IAN and lingual nerve, root damage, and fistula. The patients who were evaluated included 2 patients with inadequate fracture, 3 patients with injury to the IAN, and 1 patient with fistula. Postoperative complications (e.g., as damage of the inferior alveolar nerve and velopharyngeal insufficiency) may occur, and all patients recovered during the follow-up period of 6 months or more after the surgery. The relapse rates were A-N per 14.0%, Pog-N per 15.1%, SNA 24.4%, and SNB 4.6%. There was no statistically significant difference in relapse rate.

Conclusion: Complications that may occur after the orthognathic surgery in the patients with cleft lip/palate are similar to those without cleft lip/palate.
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http://dx.doi.org/10.1097/SCS.0000000000005536DOI Listing
September 2019

Mandibular reconstruction with a ready-made type and a custom-made type titanium mesh after mandibular resection in patients with oral cancer.

Maxillofac Plast Reconstr Surg 2018 Dec 25;40(1):35. Epub 2018 Nov 25.

Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, 20, Geumo-ro, Mulgeum-eup, Yangsan, Gyeongsangnam-do South Korea.

Background: After the resection at the mandibular site involving oral cancer, free vascularized fibular graft, a type of vascularized autograft, is often used for the mandibular reconstruction. Titanium mesh (T-mesh) and particulate cancellous bone and marrow (PCBM), however, a type of non-vascularized autograft, can also be used for the reconstruction. With the T-mesh applied even in the chin and angle areas, an aesthetic contour with adequate strength and stable fixation can be achieved, and the pores of the mesh will allow the rapid revascularization of the bone graft site. Especially, this technique does not require microvascular training; as such, the surgery time can be shortened. This advantage allows older patients to undergo the reconstructive surgery.

Case Presentation: Reported in this article are two cases of mandibular reconstruction using the ready-made type and custom-made type T-mesh, respectively, after mandibular resection. We had operated double blind peer-review process. A 79-year-old female patient visited the authors' clinic with gingival swelling and pain on the left mandibular region. After wide excision and segmental mandibulectomy, a pectoralis major myocutaneous flap was used to cover the intraoral defect. Fourteen months postoperatively, reconstruction using a ready-made type T-mesh (Striker-Leibinger, Freibrug, Germany) and iliac PCBM was done to repair the mandible left body defect.Another 62-year-old female patient visited the authors' clinic with pain on the right mandibular region. After wide excision and segmental mandibulectomy on the mandibular squamous cell carcinoma (SCC), reconstruction was done with a reconstruction plate and a right fibula free flap. Sixteen months postoperatively, reconstruction using a custom-made type T-mesh and iliac PCBM was done to repair the mandibular defect after the failure of the fibula free flap. The CAD-CAM T-mesh was made prior to the operation.

Conclusions: In both cases, sufficient new-bone formation was observed in terms of volume and strength. In the CAD-CAM custom-made type T-mesh case, especially, it was much easier to fix screws onto the adjacent mandible, and after the removal of the mesh, the appearance of both patients improved, and the neo-mandibular body showed adequate bony volume for implant or prosthetic restoration.
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http://dx.doi.org/10.1186/s40902-018-0175-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6261084PMC
December 2018

Three-Dimensional Analysis of Perioperative Condylar Displacement After Mandibular Setback Surgery With Intended Manual Condylar Positioning.

J Craniofac Surg 2018 Nov;29(8):e767-e773

Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, Yangsan, Republic of Korea.

Purpose: The purpose of this study is to investigate the effect of orthognathic surgery with intended manual condylar positioning on condyle by examining a change in its position.

Method: About 18 patients with mandibular prognathism who underwent orthognathic surgery with intentional manual condyle positioning were included. Condyle displacement was analyzed through 3D cone-beam computed tomography before and after operation. The patients were categorized into 2 experimental groups: group A (1-jaw) and group B (2-jaw). The experimental groups were examined before surgery (T0), 3 days (T1), and 6 months (T2) after surgery. Condylar displacement direction was investigated in terms of bodily shift and rotational movement.

Results: Downward bodily shift of condyle after surgery was significantly apparent from all of the patients. Condylar bodily shift in other directions was statistically insignificant. Gross bodily shift of condyle right after surgery was anterolateral-inferior direction. In perspective of rotational movement, condyle rotated in infero-medial direction right after operation, but no significant change was presented afterwards. In addition, no significant difference in the amounts of condylar shift and pattern existed between groups A and B.

Conclusion: Intended manual condylar positioning may minimize postoperative displacement of condyle while accomplishing skeletal stability.
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http://dx.doi.org/10.1097/SCS.0000000000004733DOI Listing
November 2018

Retrospective clinical study of multiple keratocystic odontogenic tumors in non-syndromic patients.

J Korean Assoc Oral Maxillofac Surg 2018 Jun 26;44(3):107-111. Epub 2018 Jun 26.

Institute of Translational Dental Sciences, Pusan National University, Yangsan, Korea.

Objectives: A keratocystic odontogenic tumor (KOT) is a type of odontogenic tumor that mainly occurs in the posterior mandible. Most KOTs appear as solitary lesions; however, they sometimes occur as multiple cysts. This study analyzed the clinical features of multiple KOTs.

Materials And Methods: The participants were diagnosed with KOT by biopsy with multiple surgical sites, and were patients at the Pusan National University Hospital and the Pusan National University Dental Hospital from January 1, 2005 to March 31, 2016. Charts, records, images and other findings were reviewed.

Results: A total of 31 operations were conducted in 17 patients. The mean patient age was 28.4±20.1 years. Multiple KOTs were found to occur at a young age (<0.01). The predominant sites were in the posterior mandible (28.6%). Most cases of multiple lesions appeared in both the upper and lower jaw, and 40.3% of lesions were associated with unerupted and impacted teeth. The overall recurrence rate measured by operation site was 10.4% (8/77 sites). No patients were associated with nevoid basal cell carcinoma syndrome.

Conclusion: The pure recurrence rate was lower than estimated, but there was a higher possibility of secondary lesions regardless of the previous operation site; therefore, long-term follow-up is necessary.
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http://dx.doi.org/10.5125/jkaoms.2018.44.3.107DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6024063PMC
June 2018

Reconstruction of cheek mucosal defect with a buccal fat pad flap in a squamous cell carcinoma patient: a case report and literature review.

Maxillofac Plast Reconstr Surg 2018 Dec 25;40(1):11. Epub 2018 May 25.

2Institute of Translational Dental Sciences, Pusan National University, Yangsan, South Korea.

Background: Squamous cell carcinoma (SCC) is the most commonly occurring malignant tumor in the oral cavity. In South Korea, it occurs most frequently in the mandible, tongue, maxilla, buccal mucosa, other areas of the oral cavity, and lips. Radial forearm free flap (RFFF) is the most widely used reconstruction method for the buccal mucosal defect. The scar of the forearm donor, however, is highly visible and unsightly, and a secondary surgical site is needed when such technique is applied. For these reasons, buccal fat pad (BFP) flap has been commonly used for closing post-surgical excision sites since the recent decades because of its reliability, ease of harvest, and low complication rate.

Case Presentation: In the case reported herein, BFP flap was used to reconstruct a cheek mucosal defect after excision. The defect was completely covered by the BFP flap, without any complications.

Conclusion: Discussed herein is the usefulness of BFP flap for the repair of the cheek mucosal defect. Also, further studies are needed to determine the possibility of using BFP flap when the defect is deep, and the maximum volume that can be harvested considering the changes in volume with age.
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http://dx.doi.org/10.1186/s40902-018-0150-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5968009PMC
December 2018

The Flavonoid Jaceosidin from Induces Apoptotic Cell Death and Inhibits the Akt Pathway in Oral Cancer Cells.

Evid Based Complement Alternat Med 2018 13;2018:5765047. Epub 2018 May 13.

Department of Oral Pathology, BK21 Plus Project, School of Dentistry, Pusan National University, Yangsan, Gyeongnam 50612, Republic of Korea.

Jaceosidin is a single compound from the Japanese mugwort , which is used as a food and a traditional medicinal herb. extracts and flavonoid components have been shown to have antihyperglycaemic, antioxidant, and anti-inflammatory properties. Although the anticancer properties of these extracts were recently demonstrated, the related mechanisms have not been characterised. In this study, we investigated the effects of jaceosidin in oral squamous cell carcinoma (OSCC) cells and initially showed selective suppression of proliferation (IC = 82.1 M in HSC-3 cells and 97.5 M in Ca9.22 cells) and accumulation of cells at the sub-G1 stage of the cell cycle. In addition, jaceosidin increased cleavage of caspase-9 and caspase-3 in OSCC cells, although caspase-8 was not detected. In further experiments, jaceosidin downregulated Akt phosphorylation and ectopic activation of Akt blocked the antiproliferative effects of jaceosidin. Finally, we showed that jaceosidin has no effects on HaCaT normal epithelial cell viability, indicating selective chemotherapeutic potential of jaceosidin and that tumour-specific downregulation of Akt increases apoptosis and inhibits growth in OSCC cells.
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http://dx.doi.org/10.1155/2018/5765047DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5971256PMC
May 2018

Evaluation of Intersegmental Displacement After Mandibular Setback Split Ramus Osteotomy Using Modified L-Shaped Monocortical Plate: Cone-Beam Computed Tomography Superimposition.

J Craniofac Surg 2018 May;29(3):655-660

Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, Yangsan, South Korea.

Purpose: The purpose of the current study is to compare intersegmental displacements after mandibular setback sagittal split ramus osteotomy (SSRO) using 4 types of osteosynthesis methods.

Patients And Methods: This is a retrospective study of 53 subjects who presented underwent bilateral setback SSRO at Pusan National University Hospital from January 2009 to December 2013. The subjects were divided into 4 groups according to the osteosynthesis method applied: group A-modified L-type monocortical plate; B-conventional miniplate; group C-bicortical screws; group D-metal and absorbable screws. To obtain the intersegmental displacement, the mean of the differences of the 3-dimensional from T0 (2 days after surgery) to T1 (6 months after surgery) was calculated for the right and left condylar heads (condylion, Cd) and the right and left coronoid processes (Cps) using 3-dimensional imaging software (Ondemand 3D; Cybermed Co, Seoul, Korea).

Results: For the condylion in the x, y, z coordinate system, in group A, there were significant differences in the y-axis for the right and left Cd; in group B, significant differences in the y-axis for the right Cd and in the y- and z-axes for the left Cd; in group C, no significant differences in the axis for the Cd; and in group D, there were significant differences in the y- and z-axes for the right Cd and in the x- and y-axes for the left Cd. For the Cps, the results are not much different from the condylion movement in all group.

Conclusion: In the current study, group C manifested the greatest displacement for the healing period. Group A did not show the significant difference to group B. In view of these results, modified L-shaped monocortical plate can be applied for osteosynthesis effectively.
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http://dx.doi.org/10.1097/SCS.0000000000004161DOI Listing
May 2018

Selective Killing of Melanoma Cells With Non-Thermal Atmospheric Pressure Plasma and p-FAK Antibody Conjugated Gold Nanoparticles.

Int J Med Sci 2017 4;14(11):1101-1109. Epub 2017 Sep 4.

Department of Oral Anatomy, School of Dentistry, Pusan National University, Yangsan 626-870, Republic of Korea.

Melanomas are fast growing high-mortality tumors, and specific treatments for melanomas are needed. Melanoma cells overexpress focal adhesion kinase (FAK) compared to normal keratinocytes, and we sought to exploit this difference to create a selectively lethal therapy. We combined gold nanoparticles (GNP) with antibodies targeting phosphorylated FAK (p-FAK). These conjugates (p-FAK-GNP) entered G361 melanoma cells and bound p-FAK. Treatment with p-FAK-GNP decreased the viability of G361 cells in a time dependent manner by inducing apoptosis. To maximize the preferential killing of G361 cells, non-thermal atmospheric pressure plasma was used to stimulate the GNP within p-FAK-GNP. Combined treatment with plasma and p-FAK-GNP showed much higher lethality against G361 cells than HaCaT keratinocyte cells. The p-FAK-GNP induced apoptosis over 48 hours in G361 cells, whereas plasma and p-FAK-GNP killed G361 cells immediately. This study demonstrates that combining plasma with p-FAK-GNP results in selective lethality against human melanoma cells.
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http://dx.doi.org/10.7150/ijms.20104DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5666541PMC
June 2018

Corrigendum: Relationship between disease stage and renal function in bisphosphonate-related osteonecrosis of the jaw.

J Korean Assoc Oral Maxillofac Surg 2017 Jun 28;43(3):212. Epub 2017 Jun 28.

Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, Yangsan, Korea.

[This corrects the article on p. 16 in vol. 43, PMID: 28280705.].
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http://dx.doi.org/10.5125/jkaoms.2017.43.3.212DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5529198PMC
June 2017

The effect of biomechanical stimulation on osteoblast differentiation of human jaw periosteum-derived stem cells.

Maxillofac Plast Reconstr Surg 2017 Dec 5;39(1). Epub 2017 Mar 5.

Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, Yangsan, South Korea.

Background: This study was to investigate the effect of biomechanical stimulation on osteoblast differentiation of human periosteal-derived stem cell using the newly developed bioreactor.

Methods: Human periosteal-derived stem cells were harvested from the mandible during the extraction of an impacted third molar. Using the new bioreactor, 4% cyclic equibiaxial tension force (0.5 Hz) was applied for 2 and 8 h on the stem cells and cultured for 3, 7, and 14 days on the osteogenic medium. Biochemical changes of the osteoblasts after the biomechanical stimulation were investigated. No treatment group was referred to as control group.

Results: Alkaline phosphatase (ALP) activity and ALP messenger RNA (mRNA) expression level were higher in the strain group than those in the control group. The osteocalcin and osteonectin mRNA expressions were higher in the strain group compared to those in the control group on days 7 and 14. The vascular endothelial growth factor (VEGF) mRNA expression was higher in the strain group in comparison to that in the control group. Concentration of alizarin red S corresponding to calcium content was higher in the strain group than in the control group.

Conclusions: The study suggests that cyclic tension force could influence the osteoblast differentiation of periosteal-derived stem cells under optimal stimulation condition and the force could be applicable for tissue engineering.
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http://dx.doi.org/10.1186/s40902-017-0104-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5337228PMC
December 2017

Relationship between disease stage and renal function in bisphosphonate-related osteonecrosis of the jaw.

J Korean Assoc Oral Maxillofac Surg 2017 Feb 20;43(1):16-22. Epub 2017 Feb 20.

Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, Yangsan, Korea.

Objectives: Bisphosphonate is the primary cause of bisphosphonate-related osteonecrosis of the jaw (BRONJ). Bisphosphonates are eliminated from the human body by the kidneys. It is anticipated that bisphosphonate levels in the body will increase if the kidney is in a weak state or if there is systemic disease that affects kidney function. The aim of this study was to analyze the relevance of renal function in the severity of BRONJ.

Materials And Methods: Ninety-three patients diagnosed with BRONJ in Pusan National University Dental Hospital from January 2012 to December 2014 were included in this study. All patients underwent a clinical exam, radiographs, and serologic lab test, including urine analysis. The patient's medical history was also taken, including the type of bisphosphonate drug, the duration of administration and drug holiday, route of administration, and other systemic diseases. In accordance with the guidelines of the 2009 position paper of American Association of Oral and Maxillofacial Surgeons, the BRONJ stage was divided into 4 groups, from stage 0 to 3, according to the severity of disease. IBM SPSS Statistics version 21.0 (IBM Co., USA) was used to perform regression analysis with a 0.05% significance level.

Results: BRONJ stage and renal factor (estimated glomerular filtration rate) showed a moderate statistically significant correlation. In the group with higher BRONJ stage, the creatinine level was higher, but the increase was not statistically significant. Other factors showed no significant correlation with BRONJ stage. There was a high statistically significant correlation between BRONJ stage and 'responder group' and 'non-responder group,' but there was no significant difference with the 'worsened group.' In addition, the age of the patients was a relative factor with BRONJ stage.

Conclusion: With older age and lower renal function, BRONJ is more severe, and there may be a decrease in patient response to treatment.
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http://dx.doi.org/10.5125/jkaoms.2017.43.1.16DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5342967PMC
February 2017

Effect of low-level laser therapy on bisphosphonate-treated osteoblasts.

Maxillofac Plast Reconstr Surg 2016 Dec 25;38(1):48. Epub 2016 Nov 25.

Department of Oral and Maxillofacial Surgery, Dentistry, Dong-A Medical Center, 602-715 Pusan, Republic of Korea.

Background: This study investigates the effect of alendronate-treated osteoblasts, as well as the effect of low-level laser therapy (LLLT) on the alendronate-treated osteoblasts. Bisphosphonate decreases the osteoblastic activity. Various treatment modalities are used to enhance the bisphosphonate-treated osteoblasts; however, there were no cell culture studies conducted using a low-level laser.

Methods: Human fetal osteoblastic (hFOB 1.19) cells were treated with 50 μM alendronate. Then, they were irradiated with a 1.2 J/cm low-level Ga-Al-As laser ( = 808 ± 3 nm, 80 mW, and 80 mA; spot size, 1 cm; NDLux, Seoul, Korea). The cell survivability was measured with the MTT assay. The three cytokines of osteoblasts, receptor activator of nuclear factor κB ligand (RANKL), osteoprotegerin (OPG), and macrophage colony-stimulating factor (M-CSF) were analyzed.

Results: In the cells treated with alendronate at concentrations of 50 μM and higher, cell survivability significantly decreased after 48 h ( < 0.05). After the applications of low-level laser on alendronate-treated cells, cell survivability significantly increased at 72 h ( < 0.05). The expressions of OPG, RANKL, and M-CSF have decreased via the alendronate. The RANKL and M-CSF expressions have increased, but the OPG was not significantly affected by the LLLT.

Conclusions: The LLLT does not affect the OPG expression in the hFOB cell line, but it may increase the RANKL and M-CSF expressions, thereby resulting in positive effects on osteoclastogenesis and bone remodeling.
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http://dx.doi.org/10.1186/s40902-016-0095-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5122599PMC
December 2016

Stability of simultaneously placed dental implants with autologous bone grafts harvested from the iliac crest or intraoral jaw bone.

BMC Oral Health 2015 Dec 30;15:172. Epub 2015 Dec 30.

Department of Oral and Maxillofacial Surgery, Gyeongsang National University School of Medicine, Institute of Health Science, Jinju, 660-702, Republic of Korea.

Background: Jaw bone and iliac bone are the most frequently used autologous bone sources for dental implant placement in patients with atrophic alveolar ridges. However, the comparative long-term stability of these two autologous bone grafts have not yet been investigated. The aim of this study was to compare the stability of simultaneously placed dental implants with autologous bone grafts harvested from either the iliac crest or the intraoral jaw bone for severely atrophic alveolar ridges.

Methods: In total, 36 patients (21 men and 15 women) were selected and a retrospective medical record review was performed. We compared the residual increased bone height of the grafted bone, peri-implantitis incidence, radiological density in newly generated bones (HU values), and implant stability using resonance frequency analysis (ISQ values) between the two autologous bone graft groups.

Results: Both autologous bone graft groups (iliac bone and jaw bone) showed favorable clinical results, with similar long-term implant stability and overall implant survival rates. However, the grafted iliac bone exhibited more prompt vertical loss than the jaw bone, in particular, the largest vertical bone reduction was observed within 6 months after the bone graft. In contrast, the jaw bone graft group exhibited a slower vertical bone resorption rate and a lower incidence of peri-implantitis during long-term follow-up than the iliac bone graft group.

Conclusions: These findings demonstrate that simultaneous dental implantation with the autologous intraoral jaw bone graft method may be reliable for the reconstruction of edentulous atrophic alveolar ridges.
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http://dx.doi.org/10.1186/s12903-015-0156-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4696287PMC
December 2015

FOXO1 Is Involved in the Effects of Cigarette Smoke Extract on Osteoblastic Differentiation of Cultured Human Periosteum-derived Cells.

Int J Med Sci 2015 21;12(11):881-90. Epub 2015 Oct 21.

3. Department of Oral and Maxillofacial Surgery, Gyeongsang National University School of Medicine and Gyeongsang National University Hospital, Institute of Health Sciences, Gyeongsang National University, Jinju, Republic of Korea.

Cigarette smoke is associated with delayed fracture healing, alterations in mineral content, and osteoporosis, however, its effects on osteoblastic differentiation of osteoprogenitor cells are not fully understood. In the present study, we examined the effects of cigarette smoke extract (CSE) on osteoblastic differentiation of cultured human periosteum-derived cells. We found that CSE inhibited alkaline phosphatase (ALP) activity, mineralization and Runx2 transactivation of the periosteum-derived cells. Nucleofection of RUNX2 into the periosteum-derived cells increased expression of endogenous osteocalcin (OC) and ALP genes in osteogenic induction medium and increased OC expression in non-osteogenic medium. Treatment of the periosteum-derived cells with CSE resulted in decreased phosphorylation of AKT and forkhead box protein O1 (FOXO1). The AKT phosphorylation-resistant mutant, FOXO1-A3, inhibited transcriptional activity of RUNX2 in the periosteum-derived cells. The current study suggests one mechanism by which CSE exposure leads to inhibition of osteoblastic differentiation of cultured human periosteum-derived cells.
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http://dx.doi.org/10.7150/ijms.13172DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4643079PMC
September 2016
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