Publications by authors named "Tran Van"

456 Publications

Resistance mechanisms and genetic relatedness among carbapenem-resistant isolates from three major hospitals in Hanoi, Vietnam (2011-15).

JAC Antimicrob Resist 2021 Sep 27;3(3):dlab103. Epub 2021 Jul 27.

Oxford University Clinical Research Unit, Hanoi, Vietnam.

Background: MDR bacteria including carbapenem-resistant are recognized as an important cause of hospital-acquired infections worldwide. This investigation seeks to determine the molecular characterization and antibiotic resistance genes associated with carbapenem-resistant .

Methods: We conducted WGS and phylogenetic analysis of 72 carbapenem-resistant isolated from hospital-acquired infection patients from August 2011 to March 2015 in three major hospitals in Hanoi, Vietnam.

Results: We identified three variants of IMP gene, among which was the most frequent (=34) in comparison to (=2) and (=12). We observed two isolates with imipenem MIC >128 mg/L that co-harboured and genes and seven isolates (imipenem MIC > 128 mg/L) with a gene from the same hospital. MLST data shows that these 72 isolates belong to 18 STs and phylogenetic tree analysis has divided these isolates into nine groups.

Conclusions: Our results provide evidence that not only but other IMP variants such as and genes and several STs (ST235, ST244, ST277, ST310, ST773 and ST3151) have been disseminating in healthcare settings in Vietnam. In addition, we report the emergence of two isolates belonging to ST1240 and ST3340 that harboured two important carbapenemase genes ( and ) and seven isolates belonging to ST3151 of that carried the gene in Vietnam, which could potentially cause serious restricted availability of treatment options in healthcare settings.
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http://dx.doi.org/10.1093/jacamr/dlab103DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313516PMC
September 2021

Novel and very rare causative variants in the COL7A1 gene of Vietnamese patients with recessive dystrophic epidermolysis bullosa revealed by whole-exome sequencing.

Mol Genet Genomic Med 2021 Jul 19:e1748. Epub 2021 Jul 19.

Institute of Genome Research, Vietnam Academy of Science and Technology, Hanoi, Vietnam.

Background: Dystrophic epidermolysis bullosa (DEB) is a rare inherited disorder characterized by skin fragility leading to trauma-induced subepidermal blisters and healing with scarring. DEB is caused by mutations in COL7A1, the gene encoding for type VII collagen (COLVII). The DEB inheritance trait is divided into dominant dystrophic epidermolysis bullosa (DDEB) and recessive dystrophic epidermolysis bullosa (RDEB).

Methods: Whole-exome sequencing (WES) was performed for identifying mutations in six affected individuals of five Vietnamese families.

Results: Three novel variants in total of eight variants were found in five families. The first novel variant causing glycine substitution (c.8279G>A, p.G2760E), the remaining two novel variants resulted in splice site affecting (c.4518+2delT and c.5821-2A>G). Functional analysis indicated that the splice site at c.4518+2delT resulted in a skipping of exon 43, leading to an in-frame deletion of 12 amino acids.

Conclusion: Our finding expands the spectrum of COL7A1 mutations and reports altered splicing at c.4518+2delT during the processing of the pre-mRNA. This study provides an additional scientific basis for diagnosis, genetic counseling, and prognosis purposes of EB patients.
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http://dx.doi.org/10.1002/mgg3.1748DOI Listing
July 2021

Successful Pregnancy Following Preimplantation Genetic Diagnosis of Adrenoleukodystrophy by Detection of Mutation on the Gene.

Appl Clin Genet 2021 14;14:313-319. Epub 2021 Jul 14.

Division of Military Science, Military Hospital 103, Hanoi, 12108, Vietnam.

Background: Adrenoleukodystrophy (ALD) is a rare sex-linked recessive disorder that disrupts adrenal gland function and the white matter of the nervous system. According to recent epidemiological statistics, up to this moment, the disease is the most recorded peroxisomal disorder. is a gene related to ALD, with more than 850 unique mutations have been reported. Early diagnosis of the disease would help to consult families with ALD to plan for interventions to prevent passing along the pathogenic mutations to their children.

Material And Methods: A heterozygous gene mutation related to ALD found in a Vietnamese woman was used to design primers for the polymerase chain reaction (PCR) to amplify the segment spanning the mutation. Then, combining sequencing methods for the PCR products, especially Sanger sequencing and next-generation sequencing (NGS), a protocol was developed to detect mutations on the gene to apply for the DNA samples of in-vitro fertilization (IVF) embryos biopsied at the blastocyst stage to screen for pathogenic alleles.

Results: The established protocol for PGD of ALD detected mutant alleles in 5/8 embryos (62.5%), while the remaining 3 embryos (37.5%) did not carry any mutation. One of the 3 embryos was transferred, and a healthy female baby was born after a full-term pregnancy.

Conclusion: The developed protocol was helpful for the preimplantation genetic diagnosis process to help families with the monogenic disease of ALD but wish to have healthy children.
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http://dx.doi.org/10.2147/TACG.S318884DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8286725PMC
July 2021

Genetic parameters and litter trait trends of Danish pigs in South Vietnam.

Anim Biosci 2021 Jun 23. Epub 2021 Jun 23.

Department of Animal Genetics, Institute of Animal Sciences for Southern Vietnam, Hiep Thang Quarter, Binh Thang Ward, Di An Town, Binh Duong 75000, Vietnam.

Objective: The objective of this study was to estimate the genetic parameters and various litter trait trends of Danish pigs in South Vietnam, including the number born alive (NBA), number weaned (NW), and litter weight at the 21st day (LW21).

Methods: Records of 936 Yorkshire sows with 3361 litters and 973 Landrace sows with 3161 litters were used to estimate the variance components, genetic parameters, and trends of NBA, NW, and LW21. The restricted maximum likelihood method was applied using VCE6 software to obtain the variance components and genetic parameters. Thereafter, the best linear unbiased prediction procedure with an animal model was applied using PEST software to estimate the breeding values of the studied traits.

Results: The heritability estimates were low, ranging from 0.12 to 0.21 for NBA, 0.03 to 0.04 for NW, and from 0.11 to 0.13 for LW21. The genetic correlation between the NBA and NW was relatively strong in both breeds, at 0.77 and 0.60 for Yorkshire and Landrace, respectively. Similarly, the genetic correlation between the NW and LW21 was considerably stronger in Landrace pigs (0.71) than in Yorkshire pigs (0.48). The estimates of annual genetic progress were 0.0431, 0.0233, and 0.0461 for NBA, NW, and LW21 in Landrace pigs and 0434, 0.0202, and 0.0667 for NBA, NW, and LW21 in Yorkshire pigs, respectively.

Conclusion: The positive genetic trends estimated for the additive genetic values of the selected traits indicated that the current breeding system has achieved favorable results.
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http://dx.doi.org/10.5713/ab.20.0692DOI Listing
June 2021

Ni-Catalyzed 1,2-Diarylation of Alkenyl Ketones: A Comparative Study of Carbonyl-Directed Reaction Systems.

Org Lett 2021 Jul 2;23(14):5311-5316. Epub 2021 Jul 2.

Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, United States.

A nickel-catalyzed 1,2-diarylation of alkenyl ketones with aryl iodides and arylboronic esters is reported. Ketones with a variety of substituents serve as effective directing groups, offering high levels of regiocontrol. A representative product is diversified into a wide range of useful products that are not readily accessible via existing 1,2-diarylation reactions. Preliminary mechanistic studies shed light on the binding mode of the substrate, and Hammett analysis reveals the effect of electronic factors on initial rates.
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http://dx.doi.org/10.1021/acs.orglett.1c01447DOI Listing
July 2021

Oral Pathogenic Bacteria-Inducing Neurodegenerative Microgliosis in Human Neural Cell Platform.

Int J Mol Sci 2021 Jun 28;22(13). Epub 2021 Jun 28.

Department of Biophysics, Institute of Quantum Biophysics, Sungkyunkwan University, Suwon 16419, Korea.

is a gram-negative bacterium found in the human oral cavity and is responsible for the development of chronic periodontitis as well as neurological diseases, including Alzheimer's disease (AD). Given the significance of the roles of in AD pathogenesis, it is critical to understand the underlying mechanisms of -driven neuroinflammation and their contribution to neurodegeneration. Herein, we hypothesize that produces secondary metabolites that may cause neurodegeneration through direct or indirect pathways mediated by microglia. To test our hypothesis, we treated human neural cells with bacterial conditioned media on our brain platforms and assessed microgliosis, astrogliosis and neurodegeneration. We found that bacteria-mediated microgliosis induced the production of nitric oxide, which causes neurodegeneration assessed with high pTau level. Our study demonstrated the elevation of detrimental protein mediators, CD86 and iNOS and the production of several pro-inflammatory markers from stimulated microglia. Through inhibition of LPS and succinate dehydrogenase in a bacterial conditioned medium, we showed a decrease in neurodegenerative microgliosis. In addition, we demonstrated the bidirectional effect of microgliosis and astrogliosis on each other exacerbating neurodegeneration. Overall, our study suggests that the mouth-brain axis may contribute to the pathogenesis of AD.
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http://dx.doi.org/10.3390/ijms22136925DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8269080PMC
June 2021

Electronic States of CoSi ( = 1-3) Clusters from Density Matrix Renormalization Group-CASPT2 Calculations.

Authors:
Van Tan Tran

J Phys Chem A 2021 Jul 27;125(26):5800-5810. Epub 2021 Jun 27.

Theoretical and Physical Chemistry Division, Dong Thap University, 783-Pham Huu Lau, Cao Lanh City, Dong Thap, Vietnam.

Density matrix renormalization group-CASPT2 (DMRG-CASPT2), CASPT2, and density functional theory are employed to describe the complicated geometrical and electronic structures of CoSi ( = 1-3) clusters. The active spaces of DMRG-CASPT2 are extended to 23 orbitals. The DMRG-CASPT2 method with such large active spaces is reasonable to provide highly accurate relative energies of the electronic states. The pure BP86, PBE, and TPSS functionals appear to be suitable to compute the relative energies of the electronic states of cobalt-doped silicon clusters. The leading configurations, bond distances, vibrational frequencies, normal modes, and relative energies of the electronic states are reported. The electron detachment energies of the removals of one electron from the anionic and neutral clusters are estimated. All six bands in the photoelectron spectrum of CoSi are interpreted based on the computed electron detachment energies and Franck-Condon factor simulations.
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http://dx.doi.org/10.1021/acs.jpca.1c04469DOI Listing
July 2021

Spontaneous and action potential-evoked Ca release from endoplasmic reticulum in neocortical synaptic boutons.

Cell Calcium 2021 Jun 16;97:102433. Epub 2021 Jun 16.

Eccles Institute of Neuroscience, John Curtin School of Medical Research, Canberra, ACT 2601, Australia; ANU Medical School, the Australian National University, Canberra, ACT 2601, Australia.

Although the endoplasmic reticulum (ER) is present throughout axons, and IP and ryanodine receptors are widely expressed in nerve terminals, whether Ca release from presynaptic stores contributes to action potential (AP)-evoked Ca transients remains controversial. We investigated the release of Ca from ER stores in boutons en passant of neocortical layer 5 pyramidal neurons. A hallmark of these stores is that they spontaneously release Ca at a low frequency. Using a high-affinity Ca indicator, we documented and characterised such spontaneous Ca transients (sCaTs), which occurred at a rate of ~0.2 per min and raised the intracellular Ca concentration ([Ca]) by ~2 µM in the absence of exogenous buffers. Caffeine increased the average frequency of sCaTs by 90%, without affecting their amplitude and decay kinetics. Therefore, presynaptic ryanodine receptors were likely involved. To determine if presynaptic ER stores contribute to intracellular Ca accumulation during repetitive stimulation, we measured [Ca] during 2 s long trains of APs evoked at 10-50 Hz. We found that for frequencies <20 Hz, [Ca] reached a steady state within ~500 ms after stimulation onset. However, for higher frequencies, [Ca] continued to increase with AP number, suggesting that the rate of Ca entry exceeded the rate of clearance. Comparison between measured and predicted values indicates supralinear summation of Ca. Block of the sarco/endoplasmic reticulum Ca-ATPase reduced the supralinearity of summation, without reducing the amplitude of a single AP-evoked Ca transient. Together, our results implicate presynaptic ER stores as a source of Ca during repetitive stimulation.
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http://dx.doi.org/10.1016/j.ceca.2021.102433DOI Listing
June 2021

Exhaled Nitric Oxide as a Surrogate Marker for Obstructive Sleep Apnea Severity Grading: An In-Hospital Population Study.

Nat Sci Sleep 2021 14;13:763-773. Epub 2021 Jun 14.

Bio-Medical Research Centre, Lam Dong Medical College, Dalat, Vietnam.

Purpose: Our study aimed to evaluate the relationship between exhaled nitric oxide (eNO) markers and obstructive sleep apnea (OSA) severity and verify the changes in eNO profiles among mild, moderate, and severe OSA subgroups.

Methods: This study was a cross-sectional and in-hospital population-based study. We investigated 123 OSA patients (17 mild, 23 moderate and, 83 severe OSA) in the department of respiratory diseases. Studied data included anthropometry, respiratory polygraphy, biological markers, spirometry, and multi-flow eNO measurements. Data analysis implied linear correlation, non-parametric ANOVA, and pair-wise comparison.

Results: No significant difference could be found among 3 OSA severity subgroups for FENO at - four sampling flow rates (50-350 mL/s). The bronchial production rate of NO (J'awNO) was proportionally increased, with median values of 11.2, 33.9, and 36.2 in mild, moderate, and severe OSA, respectively (p=0.010). The alveolar concentration of NO (CANO) changed with a non-linear pattern; it was increased in moderate (6.49) vs mild (7.79) OSA but decreased in severe OSA (5.20, p = 0.015). The only correction that could be established between OSA severity and exhaled nitric oxide markers is through J'AWNO (rho=0.25, p=0.02) and CANO (rho= 0.18, p=0.04). There was no significant correlation between FENO measured at three different flow rates and the OSA severity. We also found a weak but significant correlation between FENO 100 and averaged SpO2 (rho = 0.07, p= 0.03).

Conclusion: The present study showed that J'AWNO, which represents eNO derived from the central airway, is proportionally increased in more severe OSA, while eNO from alveolar space, indicated by CANO, was also associated with OSA severity and relatively lower in the most severe OSA patients. In contrast, stand-alone FENO metrics did not show a clear difference among the three severity subgroups.
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http://dx.doi.org/10.2147/NSS.S307012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8214111PMC
June 2021

Profiles of Linguistic Multicompetence in Vietnamese-English Speakers.

Am J Speech Lang Pathol 2021 07 23;30(4):1711-1727. Epub 2021 Jun 23.

School of Teacher Education, Charles Sturt University, Bathurst, New South Wales, Australia.

Purpose Speech-language pathologists work with increasing numbers of multilingual speakers; however, even when the same languages are spoken, multilingual speakers are not homogeneous. Linguistic multicompetence (aka multi-competence) considers competency across all languages and is associated with multiple demographic, migration, linguistic, and cultural factors. Method This article examines the linguistic multicompetence of adults with Vietnamese heritage living in Australia ( = 271) and factors associated with varying profiles of multilingualism. Participants completed a self-report questionnaire (available in English and Vietnamese) regarding their language proficiency and associated factors. Results Participants were largely (76.6%) first-generation migrants to Australia. Three distinct profiles of linguistic multicompetence were statistically identified using a cluster analysis: (a) Vietnamese proficient ( = 81, 31%), (b) similar proficiency ( = 135, 52%), and (c) English proficient ( = 43, 17%); that is, half were proficient in both languages. Multinomial logistic regression analyses compared participants profiled as having similar proficiency with those who were more dominant in one language. Factors associated with the Vietnamese proficient group (compared with the similar proficiency group) were that the participants used Vietnamese much more than English with different people across different situations, were more likely to believe that maintaining Vietnamese helped them communicate in English, and earned less. Participants in the English proficient group used English more than Vietnamese with different people across different situations, were more likely to have lived in English-speaking countries longer, were younger in age, and were less likely to believe that maintaining Vietnamese helped improve academic study than those with similar proficiency. Conclusion Undertaking a comprehensive language profile is an important component of any multilingual assessment to enable speech-language pathologists to develop an understanding of different presentations of linguistic multicompetence, engage in culturally responsive practice, and acknowledge that high levels of competence can be achieved across multiple languages. Supplemental Material https://doi.org/10.23641/asha.14781984.
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http://dx.doi.org/10.1044/2021_AJSLP-20-00296DOI Listing
July 2021

CRISPRloci: comprehensive and accurate annotation of CRISPR-Cas systems.

Nucleic Acids Res 2021 07;49(W1):W125-W130

Bioinformatics Group, Department of Computer Science, University of Freiburg, Georges-Koehler-Allee 106, 79110 Freiburg, Germany.

CRISPR-Cas systems are adaptive immune systems in prokaryotes, providing resistance against invading viruses and plasmids. The identification of CRISPR loci is currently a non-standardized, ambiguous process, requiring the manual combination of multiple tools, where existing tools detect only parts of the CRISPR-systems, and lack quality control, annotation and assessment capabilities of the detected CRISPR loci. Our CRISPRloci server provides the first resource for the prediction and assessment of all possible CRISPR loci. The server integrates a series of advanced Machine Learning tools within a seamless web interface featuring: (i) prediction of all CRISPR arrays in the correct orientation; (ii) definition of CRISPR leaders for each locus; and (iii) annotation of cas genes and their unambiguous classification. As a result, CRISPRloci is able to accurately determine the CRISPR array and associated information, such as: the Cas subtypes; cassette boundaries; accuracy of the repeat structure, orientation and leader sequence; virus-host interactions; self-targeting; as well as the annotation of cas genes, all of which have been missing from existing tools. This annotation is presented in an interactive interface, making it easy for scientists to gain an overview of the CRISPR system in their organism of interest. Predictions are also rendered in GFF format, enabling in-depth genome browser inspection. In summary, CRISPRloci constitutes a full suite for CRISPR-Cas system characterization that offers annotation quality previously available only after manual inspection.
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http://dx.doi.org/10.1093/nar/gkab456DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8265192PMC
July 2021

Kangaroo Island propolis types originating from two Lepidosperma species and Dodonaea humilis.

Phytochemistry 2021 Aug 1;188:112800. Epub 2021 Jun 1.

Sydney Pharmacy School, Faculty of Medicine and Health, The University of Sydney, New South Wales, Australia. Electronic address:

The endemic Australian plants Lepidosperma sp. Flinders Chase (Cyperaceae), Lepidosperma viscidum (Cyperaceae) and Dodonaea humilis (Sapindaceae) were found to be the botanical origin of three propolis types found on Kangaroo Island identified by TLC and H NMR matching of propolis and plant resin analytical profiles. Resin samples extracted from the plant, Lepidosperma sp. Flinders Chase, were chromatographically fractionated to give: methyl 3-phenyl-2-(E-cinnamoyloxy)propanoate (1), 3-(E-8-methoxy-8-oxo-3,7-dimethyloct-2-enyl)-4-hydroxy-E-cinnamic acid (2), 3-(E-6,7-dihydroxy-3,7-dimethyloct-2-enyl)-4-hydroxy-E-cinnamic acid (3), previously undescribed; and the known stilbenes, 2-prenyl-3,5-dihydroxy-E-stilbene (6) and 2-prenyl-3-methoxy-5-hydroxy-E-stilbene (7). The resin from L. viscidum gave: 5'-(E-4-hydroxy-3-methylbut-2-enyl)-4,2',4'-trihydroxydihydrochalcone (4); 5'-(E-4-hydroxy-3-methylbut-2-enyl)-4'-methoxy-4,2'-dihydroxydihydrochalcone (5), previously undescribed; and three known flavanones, farrerol (8), 5,7,3',5'-tetrahydroxy-6,8-dimethylflavanone (9) and 5,7,3',5'-tetrahydroxy-6-methylflavanone (10). The major constituent in the propolis identified as being sourced from D. humilis was identified as 6,8-diprenyl-5,7,3',4'-tetrahydroxyflavanone (11), a known compound identified in several unrelated plant species.
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http://dx.doi.org/10.1016/j.phytochem.2021.112800DOI Listing
August 2021

Knowledge, Attitudes, Practices, and Related Factors Towards COVID-19 Prevention Among Patients at University Medical Center Ho Chi Minh City, Vietnam.

Risk Manag Healthc Policy 2021 24;14:2119-2132. Epub 2021 May 24.

Tissue Engineering and Regenerative Medicine Department, School of Biomedical Engineering, International University, Ho Chi Minh City, 700000, Vietnam.

Aim: This study measured the level of knowledge, attitudes and practices towards COVID-19 prevention and examined associated factors among patients at a national tertiary general hospital in Vietnam.

Methods: Adult patients admitted to University Medical Center during research period were recruited in a cross-sectional study, which employed a convenience sampling method with a 4-component questionnaire in order to examine the patients' consciousness towards COVID-19 spreading prevention based on four aspects: demographic characteristics (10 items), knowledge (14 items), attitudes (6 items), and practices (7 items).

Results: The study involved 2769 respondents (18-90 years) with the mean age of 38.05±13.91 years. About two thirds of the respondents obtained diploma degree or higher (63.4%) and shared their living space with others (64.4%). The majority of patients settled in urban area (74.9%). All participants stayed informed about COVID-19, with the most commonly used channels like television (75.2%), the Internet (72.2%) and phone (69.8%). The vast majority showed sufficient knowledge (93.7%) and positive attitudes (76.3%). Just over half of participants remained good practiced of COVID-19 prevention (57.7%). On average, the factors of younger age, higher educational level, frequency and department of admission, and the number of COVID-19 informative channels were significantly associated with sufficient knowledge, positive attitudes, and good practices regarding preventive action against COVID-19 spreading. The optimistic attitude and having more undergoing chronic diseases were associated with the likelihood of well-practiced COVID-19 preventive measures (OR 3.63, 95% CI 1.54-8.55, p=0.003 and OR 0.86, 95% CI 0.78-0.98, p=0.02, respectively).

Conclusion: The results of this study demonstrated that the likelihood of good preventive practices in the fight against COVID-19 pandemic was influenced by attitudes and several sociodemographic factors. More drastic interventions for the prevention of COVID-19 should be widely furnished and equipped in hospitals, through various routes to maximize the efficiency and adherence to prevention practices.
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http://dx.doi.org/10.2147/RMHP.S305959DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163721PMC
May 2021

A Transient Directing Group Strategy Enables Enantioselective Multicomponent Organofluorine Synthesis.

J Am Chem Soc 2021 Jun 2;143(24):8962-8969. Epub 2021 Jun 2.

Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, United States.

The vicinal fluorofunctionalization of alkenes represents an expedient strategy for converting feedstock olefins into valuable fluorinated molecules and as such has garnered significant attention from the synthetic community; however, current methods remain limited in terms of scope and selectivity. Here we report the site-selective palladium-catalyzed three-component coupling of alkenylbenzaldehydes, arylboronic acids, and -fluoro-2,4,6-trimethylpyridinium hexafluorophosphate facilitated by a transient directing group. The synthetically enabling methodology constructs vicinal stereocenters with excellent regio-, diastereo-, and enantioselectivities, forging products that map onto bioactive compounds.
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http://dx.doi.org/10.1021/jacs.1c03178DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8222155PMC
June 2021

Calcium-dependent docking of synaptic vesicles.

Trends Neurosci 2021 07 25;44(7):579-592. Epub 2021 May 25.

Université de Paris, SPPIN-Saints Pères Paris Institute for the Neurosciences, CNRS, F-75006 Paris, France. Electronic address:

The concentration of calcium ions in presynaptic terminals regulates transmitter release, but underlying mechanisms have remained unclear. Here we review recent studies that shed new light on this issue. Fast-freezing electron microscopy and total internal reflection fluorescence microscopy studies reveal complex calcium-dependent vesicle movements including docking on a millisecond time scale. Recordings from so-called 'simple synapses' indicate that calcium not only triggers exocytosis, but also modifies synaptic strength by controlling a final, rapid vesicle maturation step before release. Molecular studies identify several calcium-sensitive domains on Munc13 and on synaptotagmin-1 that are likely involved in bringing the vesicular and plasma membranes closer together in response to calcium elevation. Together, these results suggest that calcium-dependent vesicle docking occurs in a wide range of time domains and plays a crucial role in several phenomena including synaptic facilitation, post-tetanic potentiation, and neuromodulator-induced potentiation.
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http://dx.doi.org/10.1016/j.tins.2021.04.003DOI Listing
July 2021

The Trilogy of SARS-CoV-2 in Pediatrics (Part 2): Multisystem Inflammatory Syndrome in Children.

J Pediatr Pharmacol Ther 2021 19;26(4):318-338. Epub 2021 May 19.

Multisystem Inflammatory Syndrome in Children (MIS-C) was first recognized as a novel illness in 2020 with manifestations similar to other hyperinflammatory syndromes, such as Kawasaki disease or macrophage activation syndrome. Severity varies from a self-limited febrile illness to shock requiring inotropes and mechanical ventilation. Gastrointestinal symptoms and persistent fevers are the most common clinical symptoms, with the addition of cardiac manifestations inclusive of ventricular dysfunction and coronary artery aneurysms. With no controlled trials or comparative effectiveness studies evaluating treatment of MIS-C to date, current treatment with immunomodulatory agents has mainly been derived from previous experience treating Kawasaki disease. This article provides a comprehensive review summarizing published data for the evaluation and management of MIS-C, with a focus on pharmacotherapy treatment considerations.
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http://dx.doi.org/10.5863/1551-6776-26.4.318DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139565PMC
May 2021

Characteristics, management, and prognosis of elderly patients with COVID-19 admitted in the ICU during the first wave: insights from the COVID-ICU study : Prognosis of COVID-19 elderly critically ill patients in the ICU.

Ann Intensive Care 2021 May 14;11(1):77. Epub 2021 May 14.

Sorbonne Université, INSERM, UMRS_1166-iCAN, Institute of Cardiometabolism and Nutrition, 75651, Paris Cedex 13, France.

Background: The COVID-19 pandemic is a heavy burden in terms of health care resources. Future decision-making policies require consistent data on the management and prognosis of the older patients (> 70 years old) with COVID-19 admitted in the intensive care unit (ICU).

Methods: Characteristics, management, and prognosis of critically ill old patients (> 70 years) were extracted from the international prospective COVID-ICU database. A propensity score weighted-comparison evaluated the impact of intubation upon admission on Day-90 mortality.

Results: The analysis included 1199 (28% of the COVID-ICU cohort) patients (median [interquartile] age 74 [72-78] years). Fifty-three percent, 31%, and 16% were 70-74, 75-79, and over 80 years old, respectively. The most frequent comorbidities were chronic hypertension (62%), diabetes (30%), and chronic respiratory disease (25%). Median Clinical Frailty Scale was 3 (2-3). Upon admission, the PaO/FiO ratio was 154 (105-222). 740 (62%) patients were intubated on Day-1 and eventually 938 (78%) during their ICU stay. Overall Day-90 mortality was 46% and reached 67% among the 193 patients over 80 years old. Mortality was higher in older patients, diabetics, and those with a lower PaO/FiO ratio upon admission, cardiovascular dysfunction, and a shorter time between first symptoms and ICU admission. In propensity analysis, early intubation at ICU admission was associated with a significantly higher Day-90 mortality (42% vs 28%; hazard ratio 1.68; 95% CI 1.24-2.27; p < 0·001).

Conclusion: Patients over 70 years old represented more than a quarter of the COVID-19 population admitted in the participating ICUs during the first wave. Day-90 mortality was 46%, with dismal outcomes reported for patients older than 80 years or those intubated upon ICU admission.
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http://dx.doi.org/10.1186/s13613-021-00861-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120254PMC
May 2021

Production of polyclonal antibody against the recombinant PirB protein of Vibrio parahaemolyticus.

J Genet Eng Biotechnol 2021 May 11;19(1):70. Epub 2021 May 11.

University of Science, 227 Nguyen Van Cu Street, Ward 4, District 5, Ho Chi Minh City, Vietnam.

Background: Acute hepatopancreatic necrosis disease (AHPND) is caused by toxin-producing strains of Vibrio parahaemolyticus which contain deadly binary toxins PirA and PirB encoded in pVA1 plasmid. The polyclonal antibodies against PirB protein could be used to develop immunochromatographic test strip for in-field diagnosis of AHPND.

Results: In this study, PirB gene was amplified, cloned, and expressed in E. coli. The expressed protein was detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot probed with 6xHis antibodies. Then, the recombinant PirB (rPirB) was purified using Ni-Sepharose column. Rabbits were immunized with the purified rPirB, and produced antibodies were analyzed using Ouchterlony double immunodiffusion. The antibody titration and antibody purification were performed by ELISA and affinity chromatography, respectively. Finally, antibody specificity and sensitivity were evaluated by dot blotting. The present study showed a high titer of polyclonal antibodies in rabbit serum after immunization and the titer increased steadily during the immunization schedule. The highest titer of antibody reached up to 2,560,000 with LOD of 0.1 ng/mL. The purified antibodies showed no cross-reactivity with proteins from other Vibrio species, and the detection threshold ranged from 6.25 to 12.5 ng toxin/dot.

Conclusion: This study highlights the production of high titer and specific polyclonal antibodies as an initial material towards the development of lateral-flow strip test.
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http://dx.doi.org/10.1186/s43141-021-00172-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113428PMC
May 2021

Dentinogenesis Imperfecta and Caries in Osteogenesis Imperfecta among Vietnamese Children.

Dent J (Basel) 2021 Apr 27;9(5). Epub 2021 Apr 27.

School of Odonto-Stomatology, Hanoi Medical University, Hanoi 100000, Vietnam.

Osteogenesis imperfecta (OI) is a genetic disorder characterized by increased bone fragility and low bone mass, caused mainly by mutations in collagen type I encoding genes. The current study aimed to evaluate dentinogenesis imperfecta (DI), oral manifestations and caries status of OI children. Sixty-eight children (41 males, 27 females) aged from 3 to 17 years old (mean 9 ± 4.13) participated in the study. Participants were classified into three OI type groups (I-2 cases, III-31 cases and IV-35 cases). Clinical examination and an orthopantomogram were used to obtain prevalences and associations of DI, caries status, malocclusion, crossbite, open bite, eruption, impaction and missing teeth with OI. The prevalence of DI among OI patients was 47.1%, more common in OI type III than type IV. The yellow-brown discoloration type was more vulnerable to attrition than the opalescent-grey one in the primary dentition. OI seemed not to have a high risk of caries; the prevalence of caries was 69.1%. A high incidence of malocclusion, crossbite and open bite was observed. In-depth oral information would provide valuable data for better dental management in OI patients. Parents and general doctors should pay more attention to dental care to prevent caries and premature tooth loss.
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http://dx.doi.org/10.3390/dj9050049DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144955PMC
April 2021

Evaluation of Survival, Recurrence Patterns and Adjuvant Therapy in Surgically Staged High-Grade Endometrial Cancer with Retroperitoneal Metastases.

Cancers (Basel) 2021 Apr 23;13(9). Epub 2021 Apr 23.

Division of Gynecologic Oncology, SUNY Downstate Medical Center, Brooklyn, NY 11203, USA.

Background: We seek to evaluate the difference in recurrence patterns and survival among stage IIIC high-grade endometrial cancer treated with surgery followed by adjuvant chemotherapy alone, radiation therapy alone, or both (chemoradiation).

Methods: A multicenter retrospective analysis of surgically staged IIIC HGEC receiving adjuvant therapy was conducted. HGEC was defined as grade 3 endometrioid adenocarcinoma, serous, clear cell and carcinosarcoma. Differences in the frequency of recurrence sites and treatment delays were identified using Pearson's χ test. Progression-free survival (PFS) and overall survival (OS) were calculated using Kaplan-Meier estimates.

Results: A total of 155 patients were evaluable: 41.9% carcinosarcoma, 36.8% serous, 17.4% grade 3 and 3.9% clear cell. Of these, 67.1% received chemoradiation, 25.8% received chemotherapy and 7.1% received radiation therapy. There was no difference in the frequency of treatment delays between regimens ( = 0.571). There was a trend towards greater retroperitoneal recurrence with chemotherapy (25.9%) versus chemoradiation (8.4%) and radiation therapy (7.7%) ( = 0.252). Grade 3 tumors had improved progression-free and overall survival (26 and 42 months, respectively) versus serous (17 and 30 months, respectively), carcinosarcoma (14 and 24 months, respectively) and clear cell (24 and 30 months respectively) ( = 0.002, < 0.001). Overall, chemoradiation was superior to chemotherapy and radiation therapy in PFS ( < 0.001) and OS ( < 0.001). Upon multivariate analysis, only histology and receipt of chemoradiation were independent predictors of survival.

Conclusion: The majority of stage IIIC high-grade endometrial carcinomas recurred. Chemoradiation was associated with improved survival and less retroperitoneal recurrence. Grade 3 tumors demonstrated improved survival versus other histologies regardless of adjuvant treatment modality.
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http://dx.doi.org/10.3390/cancers13092052DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8123054PMC
April 2021

Interpretation of photoelectron spectra of CoGe (n = 4, 5) clusters by multiconfigurational RASPT2 calculations.

J Mol Model 2021 Apr 23;27(5):131. Epub 2021 Apr 23.

Theoretical and Physical Chemistry Division, Dong Thap University, 783-Pham Huu Lau, Cao Lanh City, Dong Thap, Vietnam.

The low-lying electronic states CoGe (n = 4, 5) have been investigated with density functional theory and the state-of-the-art RASSCF/RASPT2 method to give assignments for the anion photoelectron spectra. The BP86 functional was employed to optimize the geometrical structures of the electronic states, while the RASSCF/RASPT2 was applied to calculate the single-point energies. With the RASSCF/RASPT2 approach, the active spaces are extended to a size of 21 orbitals for CoGe and 24 orbitals for CoGe. The ground states of CoGe are determined to be A″ and A″ of a trigonal bipyramidal structure in which the Co atom is situated at the equatorial corner of the bipyramid. The vertical detachment energies of the transitions from the anionic ground state to the neutral A″, 1A″, A', 2A″, 3A″, 1A', 2A', and 6A″ states are evaluated to be 2.29, 2.39, 2.60, 2.83, 3.17, 3.24, 3.47, and 4.00 eV. For the CoGe clusters, the ground states are computed to be A and 1A of an octahedral structure. The vertical detachment energies of the removal of one electron from the anionic ground state to result in the 1A, 1A, 2A, 1B, 1B, 4B, 4B, and 6A states are estimated to be 2.16, 2.79, 2.84, 3.06, 3.06, 3.59, 3.59, and 4.22 eV. All features in the photoelectron spectra of CoGe and CoGe are interpreted based on the computed electron detachment energies of the anionic ground states.
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http://dx.doi.org/10.1007/s00894-021-04753-wDOI Listing
April 2021

The Trilogy of SARS-CoV-2 in Pediatrics (Part 1): Acute COVID-19 in Special Populations.

J Pediatr Pharmacol Ther 2021 31;26(3):220-239. Epub 2021 Mar 31.

With the emergence of the SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) virus, the pandemic has resulted in a severe respiratory disease known as COVID-19. Data and literature are limited in the evaluation, treatment, and considerations for pediatric patients including special populations (e.g., neonates, children, immunocompromised patients, and those with sickle cell disease). There exists a need for a comprehensive review of pediatric proven and disproven treatments as therapies continue to emerge. This article evaluates the pharmacologic treatment and prevention therapies used in pediatric patients to date, including emergency use authorizations, as well as rationales for pharmacotherapies not routinely used to treat acute COVID-19 infection. It is important to note this review article is current as of January 25, 2021, given the rapid evolvement of the pandemic.
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http://dx.doi.org/10.5863/1551-6776-26.3.220DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8021247PMC
March 2021

Determinants of COVID-19 vaccine acceptance in a high infection-rate country: a cross-sectional study in Russia.

Pharm Pract (Granada) 2021 Jan-Mar;19(1):2276. Epub 2021 Mar 22.

Department of Chemistry, College of Natural Sciences, Can Tho University. Can Tho (Vietnam).

Background: COVID-19 vaccine development is proceeding at an unprecedented pace. Once COVID-19 vaccines become widely available, it will be necessary to maximize public vaccine acceptance and coverage.

Objective: This research aimed to analyze the predictors of COVID-19 vaccine acceptance in Russia.

Methods: A cross-sectional online survey was conducted among Russian adults from September 26 to November 9, 2020. Predictors of the intent to take up COVID-19 vaccination were explored using logistic regression.

Results: Out of 876 participants, 365 (41.7%) would be willing to receive the vaccine if it became available. Acceptance increased for a vaccine with verified safety and effectiveness (63.2%). Intention to receive the COVID-19 vaccine was relatively higher among males (aOR=2.37, 95% CI 1.41-4.00), people with lower monthly income (aOR=2.94, 95%CI 1.32-6.57), and with positive trust in the healthcare system (aOR=2.73, 95% CI 1.76-4.24). The Russian people were more likely to accept the COVID-19 vaccine if they believed that the vaccine reduces the risk of virus infection (aOR=8.80, 95%CI 5.21-14.87) or relieves the complications of the disease (aOR=10.46, 95%CI 6.09-17.96). Other barriers such as being unconcerned about side-effects (aOR=1.65, 95%CI 1.03-2.65) and the effectiveness and safety of the vaccination (aOR=2.55, 95%CI 1.60-4.08), also affected acceptance.

Conclusions: The study showed the usefulness of the health belief model constructs in understanding the COVID-19 vaccination acceptance rate in the Russian population. This rate was influenced by sociodemographic and health-related characteristics, and health beliefs. These findings might help guide future efforts for policymakers and stakeholders to improve vaccination rates by enhancing trust in the healthcare system.
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http://dx.doi.org/10.18549/PharmPract.2021.1.2276DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8005327PMC
March 2021

Investigation of ANN architecture for predicting shear strength of fiber reinforcement bars concrete beams.

PLoS One 2021 2;16(4):e0247391. Epub 2021 Apr 2.

University of Transport Technology, Hanoi, Vietnam.

In this paper, an extensive simulation program is conducted to find out the optimal ANN model to predict the shear strength of fiber-reinforced polymer (FRP) concrete beams containing both flexural and shear reinforcements. For acquiring this purpose, an experimental database containing 125 samples is collected from the literature and used to find the best architecture of ANN. In this database, the input variables consist of 9 inputs, such as the ratio of the beam width, the effective depth, the shear span to the effective depth, the compressive strength of concrete, the longitudinal FRP reinforcement ratio, the modulus of elasticity of longitudinal FRP reinforcement, the FRP shear reinforcement ratio, the tensile strength of FRP shear reinforcement, the modulus of elasticity of FRP shear reinforcement. Thereafter, the selection of the appropriate architecture of ANN model is performed and evaluated by common statistical measurements. The results show that the optimal ANN model is a highly efficient predictor of the shear strength of FRP concrete beams with a maximum R2 value of 0.9634 on the training part and an R2 of 0.9577 on the testing part, using the best architecture. In addition, a sensitivity analysis using the optimal ANN model over 500 Monte Carlo simulations is performed to interpret the influence of reinforcement type on the stability and accuracy of ANN model in predicting shear strength. The results of this investigation could facilitate and enhance the use of ANN model in different real-world problems in the field of civil engineering.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0247391PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8018664PMC
April 2021

Grain filling in barley relies on developmentally controlled programmed cell death.

Commun Biol 2021 03 30;4(1):428. Epub 2021 Mar 30.

Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Seeland, Germany.

Cereal grains contribute substantially to the human diet. The maternal plant provides the carbohydrate and nitrogen sources deposited in the endosperm, but the basis for their spatial allocation during the grain filling process is obscure. Here, vacuolar processing enzymes have been shown to both mediate programmed cell death (PCD) in the maternal tissues of a barley grain and influence the delivery of assimilate to the endosperm. The proposed centrality of PCD has implications for cereal crop improvement.
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http://dx.doi.org/10.1038/s42003-021-01953-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009944PMC
March 2021

Central versus peripheral lesion on chest X-Ray: A case series of 31 endobronchial tuberculosis patients with negative sputum smears.

Int J Mycobacteriol 2021 Jan-Mar;10(1):89-92

Department of Internal Medicine, University of Medicine and Pharmacy at Ho Chi Minh City, Vietnam.

Background: Clinical characteristics of endobronchial tuberculosis (EBTB) patients whose sputum smears were negative have not been elucidated yet.

Method: EBTB patients with negative sputum smears were documented retrospectively at the outpatient pulmonary clinic from late 2015 to early 2019.

Results: We described the characteristics of 31 EBTB patients with negative sputum smears. The median age was 36 years (range 18-81 years). The male-to-female ratio is 1:1.58. The "peripheral" lesion group included 16 cases with opacity/consolidation, 2 cases with atelectasis, 1 case with cavitary lesion, and 1 case with pleural effusion. The "central" lesion group included four cases with normal chest X-ray and seven cases with only unilateral hilar enlargement. EBTB patients with "central" lesion were more common the presence of cough, the positive rate of bronchial lavage acid-fast bacilli smear, and the rate of misdiagnosis as pharyngitis, bronchitis, or asthma than that with "peripheral" lesion.

Conclusions: EBTB with negative sputum smears was found in adult patients at any age and predominant in females. The diagnosis of EBTB with "central" lesion was more difficult than that with "peripheral" lesion. The location of the lesion could play a role in inducing cough among EBTB patients.
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http://dx.doi.org/10.4103/ijmy.ijmy_216_20DOI Listing
March 2021

Association of the STAT4, CDKN1A, and IRF5 variants with risk of lupus nephritis and renal biopsy classification in patients in Vietnam.

Mol Genet Genomic Med 2021 Apr 9;9(4):e1648. Epub 2021 Mar 9.

Center for Gene and Protein Research, Hanoi Medical University, Hanoi, Vietnam.

Background: Lupus nephritis is a common complication of systemic lupus erythematosus (SLE, OMIM #15200) in the Asian population and a main contributor to mortality and morbidity. In this study, we evaluate the variants on three genes STAT4, CDKN1A, and IRF5 and their association with lupus nephritis.

Method: One hundred fifty-two SLE patients with confirmed lupus nephritis (through biopsy) and 76 healthy controls were recruited. Genotyping of SNPs on three gene STAT4, CDKN1A, and IRF5, phenotypic, and laboratory assessment were performed; renal biopsy and classification were carried out for the patient group.

Results: Carriers of rs7582694 C alleles on STAT4 have higher risk of lupus nephritis (OR 2.0; 95% CI [1.14, 3.19]; p = 0.015), at higher risk of hematuria and higher serum level of dsDNA antibodies compared to controls (p < 0.05) and were more likely to have nephrotic histopathology grading of class III or higher. No association was observed for CDKN1A; and no variation was observed for the IRF5 gene in both the study and control group.

Conclusion: This study investigates the relationship between STAT4, CDKN1A, and IRF5 gene and SLE in a Vietnamese patient population. Patients with the C allele (STAT4) in rs7582694 were associated with a more severe disease phenotype.
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http://dx.doi.org/10.1002/mgg3.1648DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8123735PMC
April 2021

Generation and evaluation of polyclonal antibodies specific for ToxA from causing acute hepatopancreatic necrosis disease (AHPND) in shrimp.

Mol Biol Res Commun 2021 Mar;10(1):23-32

Department of Molecular and Environmental Biotechnology, Faculty of Biology and Biotechnology, University of Science, Ho Chi Minh, Vietnam.

Acute Hepatopancreatic Necrosis Disease (AHPND) is a newly emerging shrimp disease with mortality up to 100 percent caused by which carries a plasmid encoding for two toxins, ToxA and ToxB. In 2013, the Global Aquaculture Alliance (GAA) estimated shrimp farming decline in Asia accounted for 1-billion US dollar lost. Currently, diagnosis using PCR method does not meet the demand of detection, which is based on antigen-antibody interaction, has not been developed yet. In this present study, we proceeded to create the toxin and its antibody for lateral flow development. First, recombinant toxin ToxA was generated by gene manipulation. After that, purified ToxA was used to immunize rabbits. Finally, antisera from rabbits and protein-A purified antibodies were evaluated for titer, specificity, and detection threshold. Results showed that recombinant ToxA was overexpressed in soluble fraction at 37C with 1mM IPTG. Purification by affinity chromatography was able to isolate recombinant ToxA with the purity up to 94.49%. In ELISA experiment, the immunized antisera reached a titer of up to 1/5,210,000 with 1µg/ml of antigen, and detection threshold was 100ng recombinant toxin. After purification, the detection threshold of purified polyclonal antibodies was 25ng toxin per dot. These results laid a groundwork for the development of AHPND detection kit based on antigen - antibody interactions.
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http://dx.doi.org/10.22099/mbrc.2020.38774.1561DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7936389PMC
March 2021

Pharmacokinetics of Tobramycin Administered at the Beginning of Intermittent Hemodialysis Session (ESRD Study).

Can J Kidney Health Dis 2021 19;8:2054358120987061. Epub 2021 Feb 19.

Service de Néphrologie, Hôpital Maisonneuve-Rosemont, Centre intégré universitaire de santé et de services sociaux de l'Est-de-l'Île-de-Montréal, QC, Canada.

Background And Objectives: There is a renewed interest in the successful use of aminoglycosides due to increasing resistance in gram-negative infections. Few studies to date have examined the pharmacokinetics (PK) of intradialytic infusions of tobramycin. This study sought to characterize the pharmacokinetic profile of intradialytically administered tobramycin in infected patients receiving chronic intermittent hemodialysis and to determine whether it is possible to achieve favorable PK targets.

Design Setting Participants And Measurements: In this prospective pharmacokinetic study, a single dose (5 mg/kg) of tobramycin was administered intradialytically to 11 noncritically ill patients undergoing chronic intermittent hemodialysis. Blood samples were collected at selected time to determine tobramycin serum concentrations. The PK analysis was performed using Phoenix™ NLME. The efficacy exposure outcome for nonsevere gram-negative infections sensitive to tobramycin with a minimum inhibitory concentration ≤1 were maximum concentration (Cmax ≥ 10 mg/L) and area under the curve (AUC24 h > 30 mg⋅h/L). For toxicity, the goal was to identify plasma trough concentrations <2 mg/L.

Results: Tobramycin disposition was best described by a one-compartment model using a total clearance composed of the systemic clearance and a transitory hemodialysis clearance. Tobramycin mean (SD) C, trough levels, and AUC were 13.1 (1.3) mg/L, 1.32 (0.47) mg/L, and 61 (23) mg⋅h/L, respectively. Monte Carlo simulation run with 1000 virtual patients showed that a 5 mg/kg dose of tobramycin administered intradialytically can outperformed the usual low-dose postdialysis dosing (80% meeting all targets versus <1%, respectively).

Conclusions: A single high dose of tobramycin can achieve favorable PK outcome when administered using intradialytic infusions in hemodialysis patients. This practical dosing regimen may represent an effective and safer alternative to the usual dosing in the treatment of nonsevere gram-negative infections.
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http://dx.doi.org/10.1177/2054358120987061DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7897820PMC
February 2021

Edge-Rich Interconnected Graphene Mesh Electrode with High Electrochemical Reactivity Applicable for Glucose Detection.

Nanomaterials (Basel) 2021 Feb 17;11(2). Epub 2021 Feb 17.

Department of Physics, National Chung Cheng University, Chiayi 621, Taiwan.

The development of graphene structures with controlled edges is greatly desired for understanding heterogeneous electrochemical (EC) transfer and boosting EC applications of graphene-based electrodes. We herein report a facile, scalable, and robust method to produce graphene mesh (GM) electrodes with tailorable edge lengths. Specifically, the GMs were fabricated at 850 °C under a vacuum level of 0.6 Pa using catalytic nickel templates obtained based on a crack lithography. As the edge lengths of the GM electrodes increased from 5.48 to 24.04 m, their electron transfer rates linearly increased from 0.08 to 0.16 cm∙s, which are considerably greater than that (0.056 ± 0.007 cm∙s) of basal graphene structures (defined as zero edge length electrodes). To illustrate the EC sensing potentiality of the GM, a high-sensitivity glucose detection was conducted on the graphene/Ni hybrid mesh with the longest edge length. At a detection potential of 0.6 V, the edge-rich graphene/Ni hybrid mesh sensor exhibited a wide linear response range from 10.0 μM to 2.5 mM with a limit of detection of 1.8 μM and a high sensitivity of 1118.9 μA∙mM∙cm. Our findings suggest that edge-rich GMs can be valuable platforms in various graphene applications such as graphene-based EC sensors with controlled and improved performance.
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http://dx.doi.org/10.3390/nano11020511DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7922656PMC
February 2021
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