Publications by authors named "Torsak Seelanan"

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Using 6-gingerol content and gene mapping for identification of two types of ginger used in Thai traditional medicine.

J Med Assoc Thai 2012 Jan;95 Suppl 1:S135-41

Department of Applied Thai Traditional Medicine, Faculty of medicine, Thammasat University, Rungsit Campus, Klongluang, Pathumthani, Thailand.

Background: Two species of ginger were used in Thai traditional medicine as Zingiber officinale Roscoe and Zingiber ligulatum Roxb.

Objective: To investigate for identification two types of ginger by morphological and microscopic characters, DNA profiles, and determination of 6-gingerol content by HPLC.

Material And Method: Fresh rhizomes of two gingers, their ages more than one year were collected from 12 sources in 4 parts of Thailand. The fresh leaves were also collected for studying DNA profile by amplified fragment length polymorphism (AFLP) method.

Results: The morphological characters of two types of gingers were almost corresponded to AFLP patterns and they were identified as Z officinale Rocs. and Z. ligulatum Roxb. Microscopic examinations of dried rhizomes from the both species showed the same pattern. By means of HPLC and TLC methods, 6-gingerol content was found only in Z. officanale in range of 2.58-17.04% but disappeared in Z. ligulatum.

Conclusion: Determination of 6-gingerol content by HPLC or TLC pattern can be used to identified two types of ginger used in Thai Traditional Medicine.
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January 2012