Publications by authors named "Tong Zhou"

818 Publications

Catalytic Atroposelective Electrophilic Amination of Indoles.

Angew Chem Int Ed Engl 2022 May 25. Epub 2022 May 25.

Huazhong University of Science and Technology, School of Chemistry and Chemical Engineering, Luoyu road 1037, 430074, Wuhan, CHINA.

Reported here is the first catalytic atroposelective electrophilic amination of indoles, which delivers functionalized atropochiral N-sulfonyl-3-arylaminoindoles with excellent optical purity. This reaction was furnished by 1,6-nucleophilic addition to p -quinone diimines. Control experiments suggest an ionic mechanism that differs from the radical addition pathway commonly proposed for 1,6-addition to quinones. The origin of 1,6-addition selectivity was investigated through computational studies. Preliminary studies show that the obtained 3-aminoindoles atropisomers exhibit anticancer activities. This method is valuable with respect to enlarging the toolbox for atropochiral amine derivatives.
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http://dx.doi.org/10.1002/anie.202205159DOI Listing
May 2022

An Integrated Immune-Related Bioinformatics Analysis in Glioma: Prognostic Signature's Identification and Multi-Omics Mechanisms' Exploration.

Front Genet 2022 3;13:889629. Epub 2022 May 3.

Department of Emergency Medicine, Shangrao Hospital Affiliated to Nanchang University, Shangrao People's Hospital, Shangrao, China.

As the traditional treatment for glioma, the most common central nervous system malignancy with poor prognosis, the efficacy of high-intensity surgery combined with radiotherapy and chemotherapy is not satisfactory. The development of individualized scientific treatment strategy urgently requires the guidance of signature with clinical predictive value. In this study, five prognosis-related differentially expressed immune-related genes (PR-DE-IRGs) (CCNA2, HMGB2, CASP3, APOBEC3C, and BMP2) highly associated with glioma were identified for a prognostic model through weighted gene co-expression network analysis, univariate Cox and lasso regression. Kaplan-Meier survival curves, receiver operating characteristic curves and other methods have shown that the model has good performance in predicting the glioma patients' prognosis. Further combined nomogram provided better predictive performance. The signature's guiding value in clinical treatment has also been verified by multiple analysis results. We also constructed a comprehensive competing endogenous RNA (ceRNA) regulatory network based on the protective factor BMP2 to further explore its potential role in glioma progression. Numerous immune-related biological functions and pathways were enriched in a high-risk population. Further multi-omics integrative analysis revealed a strong correlation between tumor immunosuppressive environment/IDH1 mutation and signature, suggesting that their cooperation plays an important role in glioma progression.
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http://dx.doi.org/10.3389/fgene.2022.889629DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9114310PMC
May 2022

Ionic Dopant-Free Polymer Alloy Hole Transport Materials for High-Performance Perovskite Solar Cells.

J Am Chem Soc 2022 May 20. Epub 2022 May 20.

The Centre of Nanoscale Science and Technology and Key Laboratory of Functional Polymer Materials, Institute of Polymer Chemistry, College of Chemistry and Renewable Energy Conversion and Storage Center (RECAST), Nankai University, Tianjin 300071, China.

The dominated hole transport material (HTM) used in state-of-the-art perovskite solar cells (PSCs) is Spiro-OMeTAD, which needs to be doped to improve its conductivity and mobility. The inevitable instability induced by deliquescent dopants and the necessary oxidation process in air hinders the commercialization of this technology. Here, an alloy strategy using two conjugated polymers with highly similar structures but different crystallinities for dopant-free HTM and high-performance PSCs has been demonstrated. We found that the polymeric packing and crystallinity of a polymer alloy could be regulated finely by blending the polymer PM6 and our developed polymer PMSe, which exhibits a shorter π-π stacking distance due to the improved planarity of the polymer backbone with strong C═O···Se noncovalent interactions. The structure-property relationship of the polymer alloy is investigated by theoretical and experimental analyses. The optimized PSCs using the polymer alloy HTM without any ionic dopants feature an excellent power conversion efficiency of 24.53% and a high open circuit voltage () of 1.19 V with much improved stability. This efficiency is much higher than that of the control device using doped Spiro-OMeTAD HTM (PCE = 22.54%). Our work provides a very effective strategy to design and construct dopant-free hole transport materials for highly efficient perovskite solar cells and other applications.
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http://dx.doi.org/10.1021/jacs.2c04029DOI Listing
May 2022

Does Dexmedetomidine Reduce the Risk of Atrial Fibrillation and Stroke After Adult Cardiac Surgery? A Systematic Review and Meta-analysis of Randomized Controlled Trials.

Anatol J Cardiol 2022 05;26(5):354-365

Department of Intensive Care Unit, Beijing Shijitan Hospital, Capital Medical University, Beijing, China.

Background: Postoperative atrial fibrillation is a common consequence of cardiac sur-gery with increased stroke complications and mortality. Although dexmedetomidine is thought to prevent postoperative atrial fibrillation and stroke because of its sympa-tholytic and anti-inflammatory properties, data from different studies show the effect of dexmedetomidine on postoperative atrial fibrillation and stroke uncertain in adult patients with cardiac surgery.

Methods: A database including EMBASE, PubMed, and Cochrane CENTRAL was searched for randomized controlled trials comparing dexmedetomidine with placebo or other anesthetic drugs in adult cardiac surgery. The primary outcome was the incidence of postoperative atrial fibrillation. The secondary outcomes were the incidence of postop-erative stroke, mechanical ventilation duration, intensive care unit length of stay, hospi-tal length of stay, and mortality.

Results: Eighteen trials with a total of 2933 patients were enrolled in the meta-analyses. Compared with controls, dexmedetomidine significantly reduced the incidence of post-operative atrial fibrillation [odds ratio, 0.82; 95% CI, 0.69-0.98; P = .03]. There was no sig-nificant difference between groups in stroke (odds ratio, 1.36; 95% CI, 0.59-3.16; P = .47), mechanical ventilation duration [weighted mean difference, -0.17; 95% CI, -0.35 to 0.14;P=.39], intensive care unit length of stay (weighted mean difference, -0.03; 95% CI,-0.93 to 0.87; P = .95), hospital length of stay (weighted mean difference, -0.04; 95% CI,-0.40 to 0.32; P = .83) and mortality (odds ratio, 0.72; 95% CI, 0.32-1.60; P = .42).

Conclusion: Perioperative dexmedetomidine reduced the incidence of postoperative atrial fibrillation in adult patients undergoing cardiac surgery. But there was no signifi-cant difference in the incidence of stroke, mechanical ventilation duration, intensive care unit length of stay, hospital length of stay, and mortality.
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http://dx.doi.org/10.5152/AnatolJCardiol.2022.1346DOI Listing
May 2022

A tough synthetic hydrogel with excellent post-loading of drugs for promoting the healing of infected wounds in vivo.

Mater Sci Eng C Mater Biol Appl 2021 Nov 29:112577. Epub 2021 Nov 29.

College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou 310027, China; Shanxi-Zheda Institute of Advanced Materials and Chemical Engineering, Taiyuan 030000, China; MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China. Electronic address:

Bacterial infection is a major obstacle to the wound healing process. The hydrogel dressings with a simpler structure and good antibacterial and wound healing performance are appealing for clinical application. Herein, a robust hydrogel was synthesized from acrylamide (AM), acrylic acid (AA) and N,N'-methylene diacrylamide (MBA) via a redox initiating polymerization. The polymerization conditions were optimized to obtain the hydrogel with minimum unreacted monomers, which were 0.25% and 0.12% for AM and AA, respectively. The hydrogel had good mechanical strength, and could effectively resist damage by external forces and maintain a good macroscopic shape. It showed large water uptake capacity, and could post load a wide range of molecules via hydrogen bonding and electrostatic interaction. Loading of antibiotic doxycycline (DOX) enabled the hydrogel with good antibacterial activity against both Gram-positive bacteria and Gram-negative bacteria in vitro and in vivo. In a rat model of methicillin-resistant Staphylococcus aureus (MRSA)-infected full-thickness skin defect wound, the DOX-loaded hydrogel showed good therapeutic effect. It could significantly promote the wound closure, increased the collagen coverage area, down-regulate the expressions of pro-inflammatory TNF-α and IL-1β factors, and up-regulate the expressions of anti-inflammatory IL-4 factor and CD31 neovascularization factor.
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http://dx.doi.org/10.1016/j.msec.2021.112577DOI Listing
November 2021

Single-cell transcriptome highlights a multilayer regulatory network on an invasive trajectory within colorectal cancer progression.

J Cancer Res Clin Oncol 2022 May 6. Epub 2022 May 6.

Department of Biomedical Engineering, Falcuty of Environmental and Life Sciences, Beijing University of Technology, Beijing, 100124, China.

Purpose: Colorectal cancer (CRC) is one of the most common and fatal gastrointestinal malignancies, in which cancer stem cells (CSCs) were identified to enable tumor heterogeneity and initiate tumor formation. However, the process from CSCs to invasion cells is unconfirmed.

Methods: Several bioinformatics methods, including clustering, pseudotime analysis, gene set variation analysis and gene ontology enrichment, were used to construct a path of gradual transformation of CSCs to invasive cells, called "stem-to-invasion path". A large amount of signaling interactions were collated to build the multilayer regulatory network. Kaplan-Meier curve and time-dependent ROC method were applied to reveal prognostic values.

Results: We validated the heterogeneity of cells in the tumor microenvironment and revealed the presence of malignant epithelial cells with high invasive potential within primary colonic carcinomas. Next, the "stem-to-invasion path" was identified through constructing a branching trajectory with cancer cells arranged in order. A multilayer regulatory network considered as the vital factor involved in acquiring invasion characteristics underlying the path was built to elucidate the interactions between tumor cell and tumor-associated microenvironment. Then we further identified a novel combinatorial biomarker that can be used to assess the prognosis for CRC patients, and validated its predictive robustness on the independent dataset.

Conclusion: Our work provides new insights into the acquisition of invasive potential in primary tumor cells, as well as potential therapeutic targets for CRC invasiveness, which may be useful for the cancer research and clinical treatment.
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http://dx.doi.org/10.1007/s00432-022-04020-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9075720PMC
May 2022

Nucleotide Evolution, Domestication Selection, and Genetic Relationships of Chloroplast Genomes in the Economically Important Crop Genus .

Front Plant Sci 2022 15;13:873788. Epub 2022 Apr 15.

Shaanxi Key Laboratory for Animal Conservation, Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), College of Life Sciences, Northwest University, Xi'an, China.

(upland cotton) is one of the most economically important crops worldwide, which has experienced the long terms of evolution and domestication process from wild species to cultivated accessions. However, nucleotide evolution, domestication selection, and the genetic relationship of cotton species remain largely to be studied. In this study, we used chloroplast genome sequences to determine the evolutionary rate, domestication selection, and genetic relationships of 72 cotton genotypes (36 cultivated cotton accessions, seven semi-wild races of . , and 29 wild species). Evolutionary analysis showed that the cultivated tetraploid cotton genotypes clustered into a single clade, which also formed a larger lineage with the semi-wild races. Substitution rate analysis demonstrated that the rates of nucleotide substitution and indel variation were higher for the wild species than the semi-wild and cultivated tetraploid lineages. Selection pressure analysis showed that the wild species might have experienced greater selection pressure, whereas the cultivated cotton genotypes underwent artificial and domestication selection. Population clustering analysis indicated that the cultivated cotton accessions and semi-wild races have existed the obviously genetic differentiation. The nucleotide diversity was higher in the semi-wild races compared with the cultivated genotypes. In addition, genetic introgression and gene flow occurred between the cultivated tetraploid cotton and semi-wild genotypes, but mainly historical rather than contemporary gene flow. These results provide novel molecular mechanisms insights into the evolution and domestication of economically important crop cotton species.
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http://dx.doi.org/10.3389/fpls.2022.873788DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9051515PMC
April 2022

A modularized high-power ultra-wideband radiation system based on the space-synthesis method.

Rev Sci Instrum 2022 Apr;93(4):044705

State Key Laboratory of Electrical Insulation and Power Equipment, School of Electrical Engineering, Xi'an Jiaotong University, Xi'an, Shaanxi 710049, China.

In this paper, a high-power ultra-wideband radiation system, composed of multiply radiation modules, is developed based on the space-synthesis method. The radiation module mainly consists of a transistorized pulser, a 2 × 2 combined antenna array, and a power divider. To improve the out parameters [the amplitude, the pulse repetition frequency (PRF), and the rise time] of the transistorized pulser based on the Marx circuit, the influence of the traveling wave process on the output pulse must be concerned. Based on the theoretical analysis, the printed circuit board circuit parameters and the circuit structure of the pulser are optimized. To improve the power synthesis efficiency, the pulse jitter characteristic of the pulser is improved by replacing the conventional base triggering mode with the collector voltage ramp triggering mode for the first-stage avalanche transistor in the pulser. The previous optimized antenna array is utilized in this radiation system, which has a better radiation performance in the prescribed aperture area. In addition, based on the gradient microstrip structure, the power divider integrated with the pulser is designed, which has the advantages of wide bandwidth, low loss, and light weight. Experimental results show that the peak effective potential rE of the radiation system of 20 radiation modules is 221.8 kV, the maximum PRF could reach 10 kHz, and the half-power radiation angles of its radiation field are about 5° in both the E plane and the H plane. More radiation modules could be integrated into the system to achieve a higher electric field in the future.
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http://dx.doi.org/10.1063/5.0085894DOI Listing
April 2022

Design of ferrocenylseleno-dopamine derivatives to optimize the Fenton-like reaction efficiency and antitumor efficacy.

RSC Adv 2021 Jul 22;11(41):25477-25483. Epub 2021 Jul 22.

School of Chemistry and Molecular Engineering, Nanjing Tech University Nanjing 211816 China

In the current study, six ferrocenylseleno-dopamine derivatives with different structural parameters were designed. Among these derivatives, F4b, containing two ferrocene units and a tertiary amine, showed anticancer activity with IC = 2.4 ± 0.4 μM for MGC-803 cells, and its studies suggested effective antitumor activity in mice bearing an MGC-803 tumor xenograft. Mechanistic study revealed that the cytotoxicity of these ferrocenylseleno-dopamine derivatives is mainly related to the Fenton-like reaction under physiological conditions, and the tertiary amine in F4b can facilitate the HO decomposition to generate toxic ˙OH which induces apoptosis through CDK-2 inactivation.
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http://dx.doi.org/10.1039/d1ra03537aDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9036967PMC
July 2021

Experimental beam combining stabilization using machine learning trained while phases drift.

Opt Express 2022 Apr;30(8):12639-12653

An 8-beam, diffractive coherent beam combiner is phase controlled by a learning algorithm trained while optical phases drift, using a differential mapping technique. Combined output power is stable to 0.4% with 95% of theoretical maximum efficiency, limited by the diffractive element.
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http://dx.doi.org/10.1364/OE.450255DOI Listing
April 2022

Maternal Anxiety Symptoms and Chinese Adolescents' Mental Health During the COVID-19 Pandemic: The Protective Role of Adolescents' Self-Compassion.

Front Psychiatry 2022 8;13:837846. Epub 2022 Apr 8.

Maxwell School of Citizenship and Public Affairs, Syracuse University, Syracuse, NY, United States.

The COVID-19 outbreak triggered dramatic changes to family life. Parents, especially mothers, were found to experience more psychological distress during the pandemic, which may have had an impact on their children's mental health. The primary goal of this study was to examine the potential protective role of adolescents' self-compassion in the relationship between maternal anxiety and adolescents' mental health during the COVID-19 pandemic. Participants included 5,720 adolescents (48.9% girls; = 11.60, = 1.36) and their mothers from Zhengzhou city, Henan province, in Mainland China. Adolescents reported their level of self-compassion, PTSD symptoms, and negative affect during the COVID-19 pandemic. Mothers reported their own anxiety symptoms and their children's depression and anxiety symptoms. Results indicated that older female adolescents reported higher levels of PTSD symptoms and negative affect and lower levels of self-compassion than their counterparts. Maternal anxiety during the COVID-19 pandemic was consistently positively associated with adolescents' psychological maladjustment. These associations were buffered by adolescents' self-compassion. Specifically, adolescents with higher levels of self-compassion were found to be less psychologically affected by their mothers' anxiety during the COVID-19 pandemic. Findings highlighted the possibility of improving adolescents' mental health through fostering their self-compassion during the COVID-19 pandemic.
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http://dx.doi.org/10.3389/fpsyt.2022.837846DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9026151PMC
April 2022

SIPA1 boosts migration and proliferation, and blocks apoptosis of glioma by activating the phosphorylation of the FAK signaling pathway.

J Med Biochem 2022 Feb;41(1):108-114

Jiamusi University, School of Stomatology, Jiamusi, China.

Background: We aimed to analyze the regulatory effects of SIPA1 (signal-induced proliferation-associated protein 1) on glioma progression and the dominant signaling pathway.

Methods: Differential level of SIPA1 in glioma and normal tissues and cells was determined. Migratory, proliferative, apoptotic and cell cycle progression changes in A172 cells with overexpression or knockdown of SIPA1 were examined. Finally, protein levels of phosphorylated FAKs in A172 cells intervened by SIPA1, and the FAK inhibitor PF562271 were detected.

Results: SIPA1 was upregulated in glioma cases. Knock-down of SIPA1 reduced migratory and proliferative rates of glioma cells, increased apoptotic cell rate, and declined cell ratio in the S phase. The knockdown of SIPA1 also downregulated cell cycle proteins. In addition, SIPA1 upregulated phosphorylated FAKs in A172 cells and thus boosted malignant phenotypes of glioma.

Conclusions: SIPA1 is upregulated in glioma that boosts migratory and proliferative potentials of glioma cells by activating the phosphorylation of the FAK signaling pathway.
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http://dx.doi.org/10.5937/jomb0-32903DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8970579PMC
February 2022

Systemic assessment of the chiral insecticide pyriproxyfen in a citrus nectar source system: Stereoselective degradation, biological effect and exposure risk.

Pest Manag Sci 2022 Apr 14. Epub 2022 Apr 14.

Institute of Plant Protection and Agro-Product Safety, Anhui Academy of Agricultural Sciences, Hefei, China.

Background: Balancing the safety and efficiency of chiral pesticides can help protect pollinators. We evaluated the stereoselective behavior, bioactivity, toxicity and exposure risk of the chiral insecticide pyriproxyfen in a citrus nectar system.

Results: Density functional theory (DFT) and ultra-performance liquid chromatography tandem mass spectroscopy (UPLC-MS/MS) were applied for absolute configuration appraisal and chiral analysis validation, respectively. The recoveries ranged from 72.3% to 100.5% with an relative standard deviation (RSD) ranging from 1.2% to 9.7%. In a field trial, we determined insecticide half-lives in citrus leaves and flowers, which were 7.0 and 8.6 days for R-(+)-pyriproxyfen, and 11.7 and 14.7 days for S-(-)-pyriproxyfen, respectively. We found that the bioactivity of R-(+)-pyriproxyfen was 3.39 and 2.37 times higher than S-(-)-pyriproxyfen against Unaspis yanonensis and Diaphorina citri nymphs, respectively. S-(-)-pyriproxyfen had 3.8 times higher acute toxicity than R-(+)-pyriproxyfen on Apis mellifera L., and its exposure risk was moderate based on the hazard quotient.

Conclusion: The phenomenon of stereoselective degradation and biological effect demonstrated that the high-risk stereoisomer of S-(-)-pyriproxyfen degraded more slowly than R-(+)-pyriproxyfen, but R-(+)-pyriproxyfen with better efficiency for target. Therefore, an increased duration of R-(+)-pyriproxyfen activity on citrus was beneficial for efficacy. Our results could guide the scientific application and evaluation of chiral pesticides on nectar plants. © 2022 Society of Chemical Industry.
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http://dx.doi.org/10.1002/ps.6926DOI Listing
April 2022

Exploring the expanding universe of small RNAs.

Nat Cell Biol 2022 Apr 12;24(4):415-423. Epub 2022 Apr 12.

Division of Biomedical Sciences, School of Medicine, University of California, Riverside, CA, USA.

The world of small noncoding RNAs (sncRNAs) is ever-expanding, from small interfering RNA, microRNA and Piwi-interacting RNA to the recently emerging non-canonical sncRNAs derived from longer structured RNAs (for example, transfer, ribosomal, Y, small nucleolar, small nuclear and vault RNAs), showing distinct biogenesis and functional principles. Here we discuss recent tools for sncRNA identification, caveats in sncRNA expression analysis and emerging methods for direct sequencing of sncRNAs and systematic mapping of RNA modifications that are integral to their function.
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http://dx.doi.org/10.1038/s41556-022-00880-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9035129PMC
April 2022

Cu(I)-Catalyzed C-H Alkenylation of Tertiary C(sp)-H Bonds of 3-Aryl Benzofuran-2()-ones to Give - and -Styrene Containing Quaternary Carbon Centers with 99/1 Regioselectivity.

J Org Chem 2022 May 12;87(9):6064-6074. Epub 2022 Apr 12.

State Key Laboratory of Chemo/Biosensing and Chemometrics, Advanced Catalytic Engineering Research Center of the Ministry of Education, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China.

The synthesis of isomerically pure olefins containing all-carbon quaternary centers is a challenging issue. Herein, we developed an efficient protocol for the synthesis of ()-olefins (27 examples, yield up to 97%, / up to 99/1) and ()-olefins (16 examples, yield up to 94%, / up to 99/1) containing all-carbon quaternary centers. This protocol is adopted for the copper-catalyzed regioselective C-H alkenylation of the tertiary C(sp)-H bond of 3-aryl benzofuran-2()-ones with alkyne and alkenes. A diverse range of functional groups in the substrates is well-tolerated, such as F, Cl, Br, Me, OMe, ester, CF, etc. A gram scale experiment was performed in good yield, and the radical mechanisms are also proposed based on the control experiments.
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http://dx.doi.org/10.1021/acs.joc.2c00325DOI Listing
May 2022

An engineered nano-liposome-human ACE2 decoy neutralizes SARS-CoV-2 Spike protein-induced inflammation in both murine and human macrophages.

Theranostics 2022 6;12(6):2639-2657. Epub 2022 Mar 6.

Division of Biomedical Sciences, School of Medicine, University of California, Riverside, CA.

Macrophages are the frontline immune cells in response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Angiotensin-converting enzyme 2 (ACE2) serves as the binding receptor to SARS-CoV-2 Spike glycoprotein for fusion and internalization into the human host cells. However, the mechanisms underlying SARS-CoV-2-elicited macrophage inflammatory responses remain elusive. Neutralizing SARS-CoV-2 by human ACE2 (hACE2) decoys has been proposed as a therapeutic approach to ameliorate SARS-CoV-2-stimulated inflammation. This study aims to investigate whether an engineered decoy receptor can abrogate SARS-CoV-2-induced macrophage inflammation. hACE2 was biotinylated to the surface of nano-liposomes (d = 100 nm) to generate Liposome-human ACE2 complex (Lipo-hACE2). Lentivirus expressing Spike protein (D614G) was also created as a pseudo-SARS-CoV-2 (Lenti-Spike). Liposome-hACE2 was used as a decoy receptor or competitive inhibitor to inhibit SARS-CoV-2 or Lenti-Spike-induced macrophage inflammation and . Both SARS-CoV-2 and Lenti-Spike stimulated strong inflammatory responses by inducing the expression of key cytokine and chemokines, including IL-1β, IL-6, TNFα, CCL-2, and CXCL-10, in murine and human macrophages , whereas Lipo-hACE2 decoy abolished these effects in macrophages. Furthermore, intravenous injection of Lenti-Spike led to increased macrophage and tissue inflammation in wild type mice, which was also abolished by Lipo-hACE2 treatment. Mechanistically, Spike protein stimulated macrophage inflammation by activating canonical NF-κB signaling. RNA sequencing analysis revealed that Lenti-Spike induced over 2,000 differentially expressed genes (DEGs) in murine macrophages, but deficiency of IκB kinase β (IKKβ), a key regulator for NF-κB activation, abrogated Lenti-Spike-elicited macrophage inflammatory responses. We demonstrated that the engineered Lipo-hACE2 acts as a molecular decoy to neutralize SARS-CoV-2 or Spike protein-induced inflammation in both murine and human macrophages, and activation of the canonical IKKβ/NF-κB signaling is essential for SARS-CoV-2-elicited macrophage inflammatory responses.
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http://dx.doi.org/10.7150/thno.66831DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8965482PMC
March 2022

Direct Full-Length RNA Sequencing Reveals an Important Role of Epigenetics During Sexual Reversal in Chinese Soft-Shelled Turtle.

Front Cell Dev Biol 2022 25;10:876045. Epub 2022 Mar 25.

Yangtze River Fisheries Research Institute, Chinese Academy of Fisheries Science, Wuhan, China.

Sex dimorphism is a key feature of Chinese soft-shelled turtle (). The males (M) have higher econosmic value than females (F) due to wider calipash and faster growth. Exogenous hormones like estradiol and methyltestosterone can induce sexual reversal to form new phenotypes (pseudo-female, PF; pseudo-male, PM) without changing the genotype. The possibility of inducing sexual reversal is particularly important in aquaculture breeding, but the underlying biological mechanisms remain unclear. Here we applied a direct RNA sequencing method with ultralong reads using Oxford Nanopore Technologies to study the transcriptome complexity in . Nanopore sequencing of the four gender types (M, F, PF, and PM) showed that the distribution of read length and gene expression was more similar between same-sex phenotypes than same-sex genotypes. Compared to turtles with an M phenotype, alternative splicing was more pronounced in F turtles, especially at alternative 3' splice sites, alternative 5' splice sites, and alternative first exons. Furthermore, the two RNA methylation modifications m5C and m6A were differentially distributed across gender phenotypes, with the M type having more modification sites in coding sequence regions, but fewer modification sites in 3'UTR regions. Quantitative analysis of enriched m6A RNAs revealed that the N6-methylated levels of , , and were significantly higher in M phenotype individuals, while the N6-methylated levels of were reduced after sexual reversal from both M and F phenotypes. Taken together, these findings reveal an important role of epigenetics during sexual reversal in Chinese soft-shelled turtles.
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http://dx.doi.org/10.3389/fcell.2022.876045DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8990255PMC
March 2022

Fusion of Majorana bound states with mini-gate control in two-dimensional systems.

Nat Commun 2022 Apr 1;13(1):1738. Epub 2022 Apr 1.

Department of Physics, University at Buffalo, State University of New York, Buffalo, NY, 14260, USA.

A hallmark of topological superconductivity is the non-Abelian statistics of Majorana bound states (MBS), its chargeless zero-energy emergent quasiparticles. The resulting fractionalization of a single electron, stored nonlocally as a two spatially-separated MBS, provides a powerful platform for implementing fault-tolerant topological quantum computing. However, despite intensive efforts, experimental support for MBS remains indirect and does not probe their non-Abelian statistics. Here we propose how to overcome this obstacle in mini-gate controlled planar Josephson junctions (JJs) and demonstrate non-Abelian statistics through MBS fusion, detected by charge sensing using a quantum point contact, based on dynamical simulations. The feasibility of preparing, manipulating, and fusing MBS in two-dimensional (2D) systems is supported in our experiments which demonstrate the gate control of topological transition and superconducting properties with five mini gates in InAs/Al-based JJs. While we focus on this well-established platform, where the topological superconductivity was already experimentally detected, our proposal to identify elusive non-Abelian statistics motivates also further MBS studies in other gate-controlled 2D systems.
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http://dx.doi.org/10.1038/s41467-022-29463-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8976011PMC
April 2022

Paravertebral abscess and bloodstream infection caused by Burkholderia pseudomallei after acupuncture: a case report.

BMC Complement Med Ther 2022 Mar 31;22(1):95. Epub 2022 Mar 31.

Department of Intensive Care Unit, the Second Hospital of Nanjing, Nanjing University of Chinese Medicine, 1-1 Zhongfu Road, Nanjing, 210003, China.

Background: Various pathogenic bacterial infections caused by acupuncture have raised widespread concern, but paravertebral abscesses and bloodstream infections of Burkholderia pseudomallei (B.pseudomallei) after acupuncture have not been reported.

Case Presentation: A 49-year-old man was admitted to hospital with recurrent back pain and fever for 1 month, along with the finding of undiagnosed diabetes. He was considered to have tuberculosis because of unrelieved high fever and pulmonary nodules. Bilateral blood culture suggested B.pseudomallei infection, MRI of the lumbar spine suggested paravertebral abscess, and the final diagnosis was paravertebral abscess and bloodstream infection after acupuncture combined with migrating lung infection. He was discharged after abscess debridement and intensive anti-infective therapy, but no further oral antibiotics were administered because of his poor adherence. More than 5 months later, he was readmitted with the urine culture findings of B.pseudomallei. No other abscess formation was observed and he received oral antibiotics for more than 3 months without recurrence.

Conclusions: Acupuncture may lead to B.pseudomallei infection in high-risk groups, and inadequate treatment can lead to recurrent infections.
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http://dx.doi.org/10.1186/s12906-022-03563-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8974153PMC
March 2022

Tandem Mass Tagging-Based Quantitative Proteomics Analysis Reveals Damage to the Liver and Brain of Exposed to Acute Hypoxia and Reoxygenation.

Antioxidants (Basel) 2022 Mar 19;11(3). Epub 2022 Mar 19.

Yangtze River Fisheries Research Institute, Chinese Academy of Fisheries, Wuhan 430223, China.

Aquaculture environments frequently experience hypoxia and subsequent reoxygenation conditions, which have significant effects on hypoxia-sensitive fish populations. In this study, hepatic biochemical activity indices in serum and the content of major neurotransmitters in the brain were altered markedly after acute hypoxia and reoxygenation exposure in silver carp (). Proteomics analysis of the liver showed that a number of immune-related and cytoskeletal organization-related proteins were downregulated, the ferroptosis pathway was activated, and several antioxidant molecules and detoxifying enzymes were upregulated. Proteomics analysis of the brain showed that somatostatin-1A (SST1A) was upregulated, dopamine-degrading enzyme catechol O methyltransferase (COMT) and ferritin, heavy subunit (FerH) were downregulated, and the levels of proteins involved in the nervous system were changed in different ways. In conclusion, these findings highlight that hypoxia-reoxygenation has potential adverse effects on growth, locomotion, immunity, and reproduction of silver carp, and represents a serious threat to liver and brain function, possibly via ferroptosis, oxidative stress, and cytoskeleton destruction in the liver, and abnormal expression of susceptibility genes for neurodegenerative disorders in the brain. Our present findings provide clues to the mechanisms of hypoxia and reoxygenation damage in the brain and liver of hypoxia-sensitive fish. They could also be used to develop methods to reduce hypoxia or reoxygenation injury to fish.
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http://dx.doi.org/10.3390/antiox11030589DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8945220PMC
March 2022

Selection of optimal models for predicting growth stress in Artemisia desertorum by comparison of linear regression and multiple neural networks: Take the construction of a green mine in the Bayan Obo mine as an example.

Ecotoxicol Environ Saf 2022 Apr 21;235:113400. Epub 2022 Mar 21.

School of Energy and Environment, Inner Mongolia University of Science and Technology, Baotou 014010, Inner Mongolia, China; Engineering Research Center of Evaluation and Restoration in the Mining Ecological Environments, Inner Mongolia University of Science and Technology, Baotou 014010, Inner Mongolia, China; Collaborative Innovation Center of Inner Mongolia Autonomous Region Carbon Neutral, Inner Mongolia University of Science and Technology, Baotou 014010, China; Collaborative Innovation Center of Integrated Exploitation of Bayan Obo Multi-Metal Resources, Inner Mongolia University of Science and Technology, Baotou 014010, China. Electronic address:

In recent years, more and more countries are focusing on the control of mining sites and the surrounding ecological environment, and the new environmental concept of green mines has been proposed. By investigating the ecological background of a mine site, pollution and ecological imbalances in the mine can be predicted, managed or transformed. This study investigated the effects of rare earth elements on plant growth in the Baotou Bayan Obo Rare Earth Mine and evaluated soil contamination and subsequent remediation through the measured plant height. Using linear regression, BP(Back Propagation) neural networks, GA-BP(Genetic Algorithm- Back Propagation) neural networks, ELM(Extreme Learning Machine) and GA-ELM(Genetic Algorithm- Extreme Learning Machine) model prediction instruments, the different rare earth solution concentrations were set as input values and the heights of Artemisia desertorum, which as the model plant, were set as output values in the prediction. The results showed that the linear regression predicted the standard error of single La(III), Ce(III) solution and compound La(III) + Ce(III) solution for Artemisia desertorum growth stress was on the high side, 7.02%- 8.92%; the efficiency range of each group of models under BP neural network, GA-BP neural network and ELM neural network were 1.15%- 2.53%, 0.85%- 1.28%, 1.76%- 3.53%; while the efficiency range under GA-ELM neural network was 0.59%- 0.68%, with average error values and predicted values close to the true values. Among them, the MAPE of GA-ELM neural network are significantly lower than other models, and the error decreases with increasing concentration of the compound solution. So GA-ELM neural network can be used as an efficient, fast and reasonable optimal model for predicting the growth stress of Artemisia desertorum in Bayan Obo mining area. The experimental results can provide a theoretical basis for assessing the risk of soil rare earth contamination in the area, evaluating the expectation of later remediation, and provide a degree of new ideas for the construction of green mines.
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http://dx.doi.org/10.1016/j.ecoenv.2022.113400DOI Listing
April 2022

Novel Prenylated Indole Alkaloids with Neuroprotection on SH-SY5Y Cells against Oxidative Stress Targeting Keap1-Nrf2.

Mar Drugs 2022 Mar 4;20(3). Epub 2022 Mar 4.

National & Local Joint Engineering Research Centre of High-Throughput Drug Screening Technology, Hubei Key Laboratory of Biotechnology of Traditional Chinese Medicine, State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430062, China.

Oxidative stress has been implicated in the etiology of Parkinson's disease (PD). Molecules non-covalently binding to the Keap1-Nrf2 complex could be a promising therapeutic approach for PD. Herein, two novel prenylated indole alkaloids asperpenazine (), and asperpendoline () with a scarce skeleton of pyrimido[1,6-]indole were discovered from the co-cultivated fungi of MCCC 3A00521 and sp. HUBU 0120. Compound exhibited potential neuroprotective activity on SH-SY5Y cells against oxidative stress. Molecular mechanism research demonstrated that inhibited Keap1 expression, resulting in the translocation of Nrf2 from the cytoplasm to the nucleus, activating the downstream genes expression of HO-1 and NQO1, leading to the reduction in reactive oxygen species (ROS) and the augment of glutathione. Molecular docking and dynamic simulation analyses manifested that interacted with Keap1 (PDB ID: 1X2R) via forming typical hydrogen and hydrophobic bonds with residues and presented less fluctuation of RMSD and RMSF during a natural physiological condition.
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http://dx.doi.org/10.3390/md20030191DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8952805PMC
March 2022

Rice Stripe Virus Modulates the Feeding Preference of Small Brown Planthopper from the Stems to Leaves of Rice Plants to Promote Virus Infection.

Phytopathology 2022 Mar 17. Epub 2022 Mar 17.

Jiangsu Academy of Agricultural Sciences, 117941, Institute of Plant Protection, Nanjing, Jiangsu, China.

Research on plant-virus-vector interactions has revealed that viruses can enhance their spread to new host plants by attracting non-viruliferous vectors to infected plants or driving viruliferous vectors to non-infected plants. However, whether viruses can also modulate the feeding preference of viruliferous vectors for different plant parts remains largely unknown. Here, by using Rice stripe virus (RSV) and its vector, the small brown planthopper (SBPH), as a model, the effect of the virus on the feeding preference of its vector was studied by calculating the number of non-viruliferous and viruliferous SBPHs settling on different parts of rice plants. The results showed that the RSV-free SBPHs significantly preferred feeding on the stems of rice plants, whereas RSV-carrying SBPHs fed more on rice leaves. Moreover, the rice plants inoculated with RSV on the leaves showed more severe symptoms with enhanced disease incidence and virus accumulation compared to rice plants inoculated at the top and bottom of stems, suggesting that the leaves are more susceptible to RSV than the stems of rice plants. These results demonstrate that RSV modulates the feeding preference of its transmitting vector SBPH from the stems to leaves of rice plants to promote virus infection. Interestingly, we also found that the leaves were more susceptible than the stems to Rice black-streaked dwarf virus (RBSDV). This study proves that the feeding preference of insect vectors can be modulated by plant viruses to facilitate virus transmission.
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http://dx.doi.org/10.1094/PHYTO-01-22-0040-RDOI Listing
March 2022

An ensemble approach to predict binding hotspots in protein-RNA interactions based on SMOTE data balancing and random grouping feature selection strategies.

Bioinformatics 2022 Mar 7. Epub 2022 Mar 7.

Falcuty of Environmental and Life Sciences, Beijing University of Technology, Beijing, 100124, China.

Motivation: The identification of binding hotspots in protein-RNA interactions is crucial for understanding their potential recognition mechanisms and drug design. The experimental methods have many limitations, since they are usually time-consuming and labor-intensive. Thus, developing an effective and efficient theoretical method is urgently needed.

Results: Here we present SREPRHot, a method to predict hotspots, defined as the residues whose mutation to alanine generate a binding free energy change ≥ 2.0 kcal/mol, while others use a cutoff of 1.0 kcal/mol to obtain balanced datasets. To deal with the dataset imbalance, Synthetic Minority Over-sampling Technique (SMOTE) is utilized to generate minority samples to achieve a dataset balance. Additionally, besides conventional features, we use two types of new features, residue interface propensity previously developed by us, and topological features obtained using node-weighted networks, and propose an effective Random Grouping feature selection strategy combined with a two-step method to determine an optimal feature set. Finally, a stacking ensemble classifier is adopted to build our model. The results show SREPRHot achieves a good performance with SEN, MCC and AUC of 0.900, 0.557 and 0.829 on the independent testing dataset. The comparison study indicates SREPRHot shows a promising performance.

Availability And Implementation: The source code is available at https://github.com/ChunhuaLiLab/SREPRHot.

Supplementary Information: Supplementary data are available at Bioinformatics online.
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http://dx.doi.org/10.1093/bioinformatics/btac138DOI Listing
March 2022

Crystal Growth Regulation of 2D/3D Perovskite Films for Solar Cells with Both High Efficiency and Stability.

Adv Mater 2022 Apr 16;34(17):e2200705. Epub 2022 Mar 16.

The Centre of Nanoscale Science and Technology and Key Laboratory of Functional Polymer Materials, Institute of Polymer Chemistry, College of Chemistry, Nankai University, Tianjin, 300071, China.

Reducing the electronic defects in perovskite films has become a substantial challenge to further boost the photovoltaic performance of perovskite solar cells. Here, 2D (NpMA) PbI perovskite and 1-naphthalenemethylammonium iodide (NpMAI) are separately introduced into the PbI precursor solutions to regulate the crystal growth in a 2D/3D perovskite film using a two-step deposition method. The (NpMA) PbI modulated perovskite film shows a significantly improved film quality with enlarged grain size from ≈500 nm to over 1000 nm, which greatly reduces the grain-boundary defects, improves the charge carrier lifetime, and hinders ionic diffusion. As a result, the best-performing device shows a high power conversion efficiency (PCE) of 24.37% for a small-area (0.10 cm ) device and a superior PCE of 22.26% for a large-area (1.01 cm ) device. Importantly, the unencapsulated device shows a dramatically improved operational stability with maintains over 98% of its initial efficiency after 1500 h by maximum power point (MPP) tracking under continuous light irradiation.
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http://dx.doi.org/10.1002/adma.202200705DOI Listing
April 2022

Analyses on the Infection Process of Rice Virus and the Spatiotemporal Expression Pattern of Host Defense Genes Based on a Determined-Part Inoculation Approach.

Pathogens 2022 Jan 24;11(2). Epub 2022 Jan 24.

Key Laboratory of Food Quality and Safety, Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.

Rice viral diseases adversely affect crop yield and quality. Most rice viruses are transmitted through insect vectors. However, the traditional whole-plant inoculation method cannot control the initial inoculation site in rice plants because the insect feeding sites in plants are random. To solve this problem, we established a determined-part inoculation approach in this study that restricted the insect feeding sites to specific parts of the rice plant. (RSV) was used as the model virus and was inoculated at the bottom of the stem using our method. Quantitative real-time PCR and Western blot analyses detected RSV only present at the bottom of the Nipponbare (NPB) stem at 1 day post-inoculation (dpi), indicating that our method successfully controlled the inoculation site. With time, RSV gradually moved from the bottom of the stem to the leaf in NPB rice plants, indicating that systemic viral spread can also be monitored using this method. In addition, a cultivar resistant to RSV, Zhendao 88 (ZD88), was inoculated using this method. We found that RSV accumulation in ZD88 was significantly lower than in NPB. Additionally, the expression level of the resistant gene in ZD88 was highly induced at the initial invasion stage of RSV (1 dpi) at the inoculation site, whereas it remained relatively stable at non-inoculated sites. This finding indicated that directly responded to RSV invasion to inhibit virus accumulation at the invasion site. We also proved that this approach is suitable for other rice viruses, such as (RBSDV). Interestingly, we determined that systemic infection with RSV was faster than that with RBSDV in NPB, which was consistent with findings in field trails. In summary, this approach is suitable for characterizing the viral infection process in rice plants, comparing the local viral accumulation and spread among different cultivars, analyzing the spatiotemporal expression pattern of resistance-associated genes, and monitoring the infection rate for different viruses.
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http://dx.doi.org/10.3390/pathogens11020144DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8880328PMC
January 2022

Developing and validating a Chinese multimorbidity-weighted index for middle-aged and older community-dwelling individuals.

Age Ageing 2022 02;51(2)

Department of Medical Statistics, School of Public Health, Sun Yat-sen University, Guangzhou, P.R. China.

Objective: To develop and validate an index to quantify the multimorbidity burden in Chinese middle-aged and older community-dwelling individuals.

Methods: We included 20,035 individuals aged 45 and older from the China Health and Retirement Longitudinal Study (CHARLS) and 19,297 individuals aged 65 and older from the Chinese Longitudinal Healthy Longevity Survey (CLHLS). Health outcomes of physical functioning (PF), basic and instrumental activities of daily living (ADL and IADL) and mortality were obtained. Based on self-reported disease status, we calculated five commonly used western multimorbidity indexes for CHARLS baseline participants. The one that predicted the health outcomes the best was selected and then modified through a linear mixed model using the repeated individual data in CHARLS. The performance of the modified index was internally and externally evaluated with CHARLS and CLHLS data.

Results: The multimorbidity-weighted index (MWI) performed the best among the five indexes. In the modified Chinese multimorbidity-weighted index (CMWI), the weights of the diseases varied greatly (range 0.2-5.1). The top three diseases with the highest impact were stroke, memory-related diseases and cancer, corresponding to weights of 5.1, 4.3 and 3.4, respectively. Compared with the MWI, the CMWI showed better model fits for PF and IADL with larger R2 and smaller Akaike information criterion, and comparable prediction performances for ADL, IADL and mortality (e.g. the same predictive accuracy of 0.80 for ADL disability).

Conclusion: The CMWI is an adequate index to quantify the multimorbidity burden for Chinese middle-aged and older community-dwelling individuals. It can be directly computed via disease status examined in regular community health check-ups to facilitate health management.
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http://dx.doi.org/10.1093/ageing/afab274DOI Listing
February 2022

Knockdown of miR-92a suppresses the stemness of colorectal cancer cells via mediating SOCS3.

Bioengineered 2022 03;13(3):5613-5624

Department of Gastrointestinal Surgery II, The Second Affiliated Hospital of North Sichuan Medical College, Sichuan, China.

Abbreviations: CRC, cancer cell; CSCs, cancer stem cells; SOCS3, suppressor of cytokine signaling 3.
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http://dx.doi.org/10.1080/21655979.2021.2022267DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8974062PMC
March 2022

In situ deletions reveal regulatory components for expression of an intracellular immune receptor gene and its co-expressed genes in Arabidopsis.

Plant Cell Environ 2022 Jun 24;45(6):1862-1875. Epub 2022 Feb 24.

School of Integrative Plant Science, Plant Biology Section, Cornell University, Ithaca, New York, USA.

Intracellular immune receptor nucleotide-binding leucine-rich repeats (NLRs) are highly regulated transcriptionally and post-transcriptionally for balanced plant defense and growth. NLR genes often exist in gene clusters and are usually co-expressed under various conditions. Despite intensive studies of the regulation of NLR proteins, cis-acting elements for NLR gene induction, repression or co-expression are largely unknown due to a larger than usual cis-region for their expression regulation. Here we used the CRISPR/Cas9 genome editing technology to generate a series of in situ deletions at the endogenous location of an NLR gene SNC1 residing in the RPP5 gene cluster. These deletions that made in the wild type and the SNC1 constitutive expressing autoimmune mutant bon1 revealed both positive and negative cis-acting elements for SNC1 expression. Two transcription factors that could bind to these elements were found to have an impact on the expression of SNC1. In addition, co-expression of two genes with SNC1 in the same cluster is found to be mostly dependent on the SNC1 function. Therefore, SNC1 expression is under complex local regulation involving multiple cis-elements and SNC1 itself is a critical regulator of gene expression of other NLR genes in the same gene cluster.
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http://dx.doi.org/10.1111/pce.14293DOI Listing
June 2022

Preparation of three dimensional bimetallic Cu-Ni/NiF electrodes for efficient electrochemical removal of nitrate nitrogen.

Chemosphere 2022 May 8;295:133929. Epub 2022 Feb 8.

Dalian (Fushun) Research Institute of Petroleum and Petrochemical, Sinopec Corp, National Engineering Research Center of Industrial Wastewater Detoxication and Resource Recovery, Dalian, 116045, China.

It still remains a hotspot and great challenge to efficiently remove the nitrate nitrogen from high salt wastewater. Herein, a novel three dimensional porous bimetallic copper-nickel alloy electrode was fabricated with Ni foam (NiF) as substrate. The physicochemical and electrochemical characterization results showed Cu-Ni/NiF electrode possessed the smaller particle size (0.3-1.0 μm) and electrode film resistance comparing with Ni/NiF and Cu/NiF electrodes. Besides, higher double layer capacitance (C) for Cu-Ni/NiF electrode indicated more electrochemical active sites could be used in the electrochemical nitrate nitrogen (NO-N) removal. The electrochemical experiments showed the Cu-Ni/NiF electrode had the optimal NO-N reduction ability and almost 100% NO-N removal could be achieved with 30 min. All NO-N removal processes were in accord with the pseudo-first-order reaction kinetics completely. The gaseous nitrogen selectivity for Cu-Ni/NiF electrode could reach 80.9% within 300 min. Stability assessment experiments indicated the Cu-Ni/NiF electrode all kept an excellent stability with NaSO or NaCl electrolyte and the Cl addition could significantly improve the gaseous nitrogen selectivity. Finally, a possible removal mechanism of NO-N was proposed. This work offered a direction for designing non-noble bimetallic electrodes for nitrate removal.
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http://dx.doi.org/10.1016/j.chemosphere.2022.133929DOI Listing
May 2022
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