Publications by authors named "Tong Liu"

1,784 Publications

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Association between leucocyte telomere length and the risk of atrial fibrillation: An updated systematic review and meta-analysis.

Ageing Res Rev 2022 Aug 3;81:101707. Epub 2022 Aug 3.

Tianjin Key Laboratory of Ionic-Molecular Function of Cardiovascular Disease, Department of Cardiology, Tianjin Institute of Cardiology, Second Hospital of Tianjin Medical University, Tianjin 300211, China. Electronic address:

Background And Aims: Advancing age is the most important risk factor of atrial fibrillation (AF). The shortening of telomere length is a biomarker of biologic aging. There is an increasing body of evidence that leucocyte telomere length (LTL) is associated with the risk of AF development. However, the results in these studies were controversial. The current systematic review and meta-analysis was conducted to examine the role of LTL in predicting the incidence of AF.

Methods And Results: Observational studies reporting the association between LTL and the risk of AF were retrieved through 25th June, 2022 from PubMed and Embase. A total of twelve studies including 18,293 patients were included in the present analysis. Leucocyte telomere shortening was found to be an independent predictor of AF as a continuous variable in both univariate [OR:2.14; 95%CI(1.48,3.10); P < 0.0001] and multivariate analyses [OR:1.41;95%CI(1.11,1.79); P = 0.005], as well as categorical variable in multivariate analysis [OR:1.53; 95%CI(1.04,2.27); P = 0.03]. Furthermore, leucocyte telomere shortening was significantly associated with recurrent AF [OR:4.32;95%CI(2.42,7.69); P < 0.00001] but not new-onset AF [OR:1.14; 95%CI(0.90,1.45); P = 0.29]. Leucocyte telomere shortening was also associated with an increased risk of persistent AF [OR:14.73;95%CI (3.16,68.67); P = 0.0006] and paroxysmal AF [OR:2.74;95%CI(1.45,5.18); P = 0.002]. Besides, LTL was an independent predictor for progression from paroxysmal AF to persistent AF [OR:3.2;95%CI(1.66,6.18); P = 0.0005]. Differences between males [OR:1.99; 95%CI(1.29,3.06); P = 0.002] and females [OR:0.86; 95%CI (0.29,2.56);P = 0.79] were observed.

Conclusions: Leucocyte telomere shortening predicts the risk of AF, especially recurrent AF. The predictive value is more prominent in males than in females. Shortening in LTL can predict the progression from paroxysmal to persistent AF.
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http://dx.doi.org/10.1016/j.arr.2022.101707DOI Listing
August 2022

Clinical Outcome of Paclitaxel-Coated Balloon Angioplasty Versus Drug-Eluting Stent Implantation for the Treatment of Coronary Drug-Eluting Stent In-Stent Chronic Total Occlusion.

Cardiovasc Drugs Ther 2022 Aug 5. Epub 2022 Aug 5.

Center for Coronary Artery Disease, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart Lung and Blood Vessel Diseases, No. 2 Anzhen Road, Chaoyang District, Beijing, 100029, China.

Aims: In-stent chronic total occlusion (IS-CTO) represents a unique challenge for percutaneous coronary intervention. Whether the optimal treatment for IS-CTO is angioplasty with paclitaxel-coated balloons (PCBs) or repeat stenting with drug-eluting stents (DESs) is unclear. We aimed to evaluate the long-term clinical outcome of PCB angioplasty and DES repeat stenting for DES IS-CTO.

Methods: We retrospectively included patients with DES IS-CTO who underwent successful PCB angioplasty or DES repeat stenting from January 2016 to December 2019. The primary endpoints were major adverse cardiac events (MACEs), including cardiac death, myocardial infarction, and target lesion revascularization (TLR). Cox proportional hazards model was performed to compare the risk of MACEs between PCB angioplasty and DES repeat stenting, and to further explore the prognostic factors of patients with DES IS-CTO.

Results: A total of 214 patients with DES IS-CTO were enrolled: 78 patients (36.4%) treated with PCB and 136 patients (63.6%) treated with DES respectively. The median follow-up was 1160 days, and MACEs were observed in 28.2% of patients with PCB angioplasty versus 26.5% of patients with DES repeat stenting (P = 0.784), mainly driven by TLR (21.8% vs. 19.9%, P = 0.735). There was no significant difference in the risk of MACEs between the PCB group and the DES group (hazard ratio [HR] 1.25, 95% confidence interval [CI] 0.64-2.46, P = 0.512). Multivariate Cox analysis revealed that chronic kidney disease and ≥ 3 stent layers in the lesion were independent predictors of MACEs, while switching to another antiproliferative drug was an independent protective factor (all P < 0.05).

Conclusions: PCB angioplasty was an effective alternative treatment strategy for DES IS-CTO, which had similar long-term outcomes to DES repeat stenting in contemporary practice, but both were accompanied by a high rate of long-term MACEs. Improving the poor prognosis of patients with DES IS-CTO remains a challenge.
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http://dx.doi.org/10.1007/s10557-022-07363-7DOI Listing
August 2022

Association between metabolic syndrome, C-reactive protein, and the risk of primary liver cancer: a large prospective study.

BMC Cancer 2022 Aug 4;22(1):853. Epub 2022 Aug 4.

Department of Gastrointestinal Surgery/Clinical Nutrition, Beijing Shijitan Hospital, Capital Medical University, Beijing, 100038, China.

Background And Aims: High-sensitivity C-reactive protein (hs-CRP) levels and metabolic syndrome (MetS) are known to be associated with an increased incidence of different cancers. We aimed to evaluate the effect of MetS combined with high hs-CRP levels on the risk of primary liver cancer (PLC).

Methods: Participants were recruited from the Kailuan cohort study and were classified into four groups according to the presence or absence of MetS and inflammation (hs-CRP ≥ 3 or < 3 mg/L). The associations of MetS and inflammation with the risk of PLC were assessed using Cox proportional hazards models.

Results: This study included 92,770 participants. The mean age was 51.4 years old. Over a median follow-up of 13.02 years, 395 participants were diagnosed as PLC. Compared to the control participants without inflammation (hs-CRP < 3 mg/L) and MetS (n = 69,413), participants with high hs-CRP levels combined with MetS (n = 2,269) had a higher risk of PLC [hazard ratios (HR) 2.91; 95% confidence interval (CI), 1.77-4.81], and participants with high hs-CRP levels and without MetS (n = 14,576) had the same trend (HR, 1.36; 95%CI, 1.05-1.75). However, participants with low hs-CRP levels and MetS (n = 6,512) had no significant association with an elevated risk of PLC (HR, 1.18; 95%CI, 0.76-1.82). After excluding participants who had cancer during the first year of follow-up, sensitivity analysis showed the same trend. In addition, co-occurrence of MetS and high hs-CRP levels had significant interactive effects on the risk of PLC between the sexes (P < 0.001) and the patients with HBV infection (P = 0.012).

Conclusions: Participants with co-occurrence of MetS and high hs-CRP levels have an elevated risk of PLC.

Trial Registration: Kailuan study, ChiCTR-TNRC-11001489. Registered 24 August, 2011-Retrospectively registered, http://www.chictr.org.cn/showprojen.aspx?proj=8050.
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http://dx.doi.org/10.1186/s12885-022-09939-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9351132PMC
August 2022

Whole cell response to receptor stimulation involves many deep and distributed subcellular biochemical processes.

J Biol Chem 2022 Aug 1:102325. Epub 2022 Aug 1.

Department of Pharmacological Sciences and Institute for Systems Biomedicine, Icahn School of Medicine at Mount Sinai, New York, NY 10029, United States. Electronic address:

Neurite outgrowth is an integrated whole-cell response triggered by the cannabinoid-1 receptor (CB1R). We sought to identify the many different biochemical pathways that contribute to this whole cell response. To understand underlying mechanisms, we identified subcellular processes composed of one or more biochemical pathways and their interactions required for this response. Differentially expressed genes and proteins were obtained from bulk transcriptomics and proteomic analysis of extracts from cells stimulated by a CB1R agonist. We then used these differentially expressed genes and proteins to build networks of SCPs and their interactions by combining the expression data with prior pathway knowledge. From these SCP networks, we identified additional genes that when ablated, experimentally validated the SCP involvement in neurite outgrowth. Our experiments and informatics modeling allowed us to identify diverse SCPs such as those involved in pyrimidine metabolism, lipid biosynthesis, and mRNA splicing and stability, along with more predictable SCPs such as membrane vesicle transport and microtubule dynamics. We find that SCPs required for neurite outgrowth are widely distributed among many biochemical pathways required for constitutive cellular functions, several of which are termed 'deep', since they are distal to signaling pathways and the key SCPs directly involved in extension of the neurite. In contrast, 'proximal' SCPs are involved in microtubule growth and membrane vesicle transport dynamics required for neurite outgrowth. From these bioinformatics and dynamical models based on experimental data, we conclude that receptor-mediated regulation of subcellular functions for neurite outgrowth is both distributed, i.e., involves many different biochemical pathways, and deep.
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http://dx.doi.org/10.1016/j.jbc.2022.102325DOI Listing
August 2022

Aspirin protects against UVB-induced DNA damage through activation of AMP kinase.

J Invest Dermatol 2022 Aug 1. Epub 2022 Aug 1.

Huntsman Cancer Institute, University of Utah Health Sciences Center, Salt Lake City, Utah; Department of Dermatology, University of Utah Health Sciences Center, Salt Lake City, Utah; Department of Oncological Sciences, University of Utah, Salt Lake City, Utah. Electronic address:

The anti-inflammatory and chemopreventive activities of aspirin (ASA) may be mediated through its cyclooxygenase (COX) inhibitor function. We have previously shown that ASA can protect against ultraviolet (UV) radiation-induced skin inflammation and DNA damage, however the role of inflammation in UV-induced DNA damage and the mechanism underlying ASA protection are poorly characterized. Using immunodeficient NSG mice and immunocompetent C57BL/6 mice treated with immune cell-depleting antibodies, we found that inflammation was not required for UVB-induced 8-oxoguanine (8-OG) and cyclobutane pyrimidine dimers (CPD) in vivo. Unlike ASA, neither its immediate metabolite salicylate nor the COX inhibitor indomethacin reduced UVB-induced 8-OG or CPD in melanocyte Melan-a or keratinocyte HaCat cells in vitro. Moreover, addition of prostaglandin-E (PGE) did not reverse the protective effect of ASA on UVB-treated cells. Phosphorylation of the 5' AMP protein kinase (AMPK), observed in ASA-treated cells, could be blocked by the AMPK inhibitor Compound C (Comp C). Comp C or AMPK knockdown partially reduced ASA-mediated protection against UVB-induced DNA damage. Finally, injection of Comp C partially reversed the protective effect of ASA on UVB-treated mouse skin in vivo. These studies suggest that ASA confers protection against UVB-induced DNA damage through activation of AMPK rather than COX inhibition.
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http://dx.doi.org/10.1016/j.jid.2022.07.011DOI Listing
August 2022

Nuclear Condensation of CDYL Links Histone Crotonylation and Cystogenesis in Autosomal Dominant Polycystic Kidney Disease.

J Am Soc Nephrol 2022 Aug 2. Epub 2022 Aug 2.

Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Tianjin Institute of Urology, The Second Hospital of Tianjin Medical University, Tianjin Medical University, Tianjin, China

Background: Emerging evidence indicates that epigenetic modulation of gene expression plays a key role in the progression of autosomal dominant polycystic kidney disease (ADPKD). However, the molecular basis for how the altered epigenome modulates transcriptional responses, and thereby disease progression in ADPKD, remains largely unknown.

Methods: Kidneys from control and ADPKD mice were examined for the expression of CDYL and histone acylations. CDYL expression and its correlation with disease severity were analyzed in a cohort of patients with ADPKD. transgenic mice were crossed with knockout mice to explore CDYL's role in ADPKD progression. Integrated cistromic and transcriptomic analyses were performed to identify direct CDYL target genes. High-sensitivity mass spectrometry analyses were undertaken to characterize CDYL-regulated histone lysine crotonylations (Kcr). Biochemical analysis and zebrafish models were used for investigating CDYL phase separation.

Results: CDYL was downregulated in ADPKD kidneys, accompanied by an increase of histone Kcr. Genetic overexpression of reduced histone Kcr and slowed cyst growth. We identified CDYL-regulated cyst-associated genes, whose downregulation depended on CDYL-mediated suppression of histone Kcr. CDYL assembled nuclear condensates through liquid-liquid phase separation in cultured kidney epithelial cells and in normal kidney tissues. The phase-separating capacity of CDYL was required for efficient suppression of locus-specific histone Kcr, of expression of its target genes, and of cyst growth.

Conclusions: These results elucidate a mechanism by which CDYL nuclear condensation links histone Kcr to transcriptional responses and cystogenesis in ADPKD.
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http://dx.doi.org/10.1681/ASN.2021111425DOI Listing
August 2022

MXene-Derived 3D Defect-Rich [email protected] Graphene Oxide Aerogel with Ultrafast Carrier Separation for Photo-Assisted Uranium Extraction: A Combined Batch, X-ray Absorption Spectroscopy, and Density Functional Theory Calculations.

Inorg Chem 2022 Aug 1. Epub 2022 Aug 1.

State Key Laboratory of Environmentally Friendly Energy Materials, National Collaborative Innovation Center for Nuclear Waste and Environmental Safety, School of National Defence, Fundamental Science on Nuclear Wastes and Environmental Safety Laboratory, Sichuan Civil-military Integration Institute, Southwest University of Science and Technology, Mianyang 621010, China.

Encapsulation of nano-semiconductor materials in three-dimensional (3D) adsorbents to build a typical semiconductor-adsorbent heterostructure is a forward-looking strategy for photo-assisted uranium extraction. Here, we develop 3D MXene-derived TiO(M)@reduced graphene oxide (RGO) aerogel for photo-assisted uranium extraction. Theoretical simulations demonstrate that oxygen vacancies on TiO(M) tailor the energy level structure and enhance the electron accumulation at gap states of TiO(M), thereby further realizing the spatial separation efficiency of electron-hole pairs by the Schottky junction. By virtue of the in situ X-ray photoelectron spectroscopy spectrum, we identify that photogenerated electrons generated over TiO(M) were transferred to graphene oxide aerogel by the Schottky junction. Accordingly, TiO (M)@RGO aerogel presents a considerable removal efficiency for U(VI) with a removal ratio of 95.7%. Relying on the X-ray absorption spectroscopy technique, we distinguish the evolution of 2HO-2O-U-5O into HO-2O-U-3O from dark to light conditions, further confirming the reduction of high-valent uranium. This strategy may open a paradigm for developing novel heterojunctions as photocatalysts for selective U(VI) extraction.
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http://dx.doi.org/10.1021/acs.inorgchem.2c01850DOI Listing
August 2022

Combining Immune-Related Genes For Delineating the Extracellular Matrix and Predicting Hormone Therapy and Neoadjuvant Chemotherapy Benefits In Breast Cancer.

Front Immunol 2022 14;13:888339. Epub 2022 Jul 14.

Department of Oncology, Harbin Medical University, Harbin, China.

Breast cancer (BC) is the most prevalent cancer in women worldwide. A systematic approach to BC treatment, comprising adjuvant and neoadjuvant chemotherapy (NAC), as well as hormone therapy, forms the foundation of the disease's therapeutic strategy. The extracellular matrix (ECM) is a dynamic network that exerts a robust biological effect on the tumor microenvironment (TME), and it is highly regulated by several immunological components, such as chemokines and cytokines. It has been established that the ECM promotes the development of an immunosuppressive TME. Therefore, while analyzing the ECM of BC, immune-related genes must be considered. In this study, we used bioinformatic approaches to identify the most valuable ECM-related immune genes. We used weighted gene co-expression network analysis to identify the immune-related genes that potentially regulate the ECM and then combined them with the original ECM-related gene set for further analysis. Least absolute shrinkage and selection operator (LASSO) regression and SurvivalRandomForest were used to narrow our ECM-related gene list and establish an ECM index (ECMI) to better delineate the ECM signature. We stratified BC patients into ECMI high and low groups and evaluated their clinical, biological, and genomic characteristics. We found that the ECMI is highly correlated with long-term BC survival. In terms of the biological process, this index is positively associated with the cell cycle, DNA damage repair, and homologous recombination but negatively with processes involved in angiogenesis and epithelial-mesenchymal transition. Furthermore, the tumor mutational burden, copy number variation, and DNA methylation levels were found to be related to the ECMI. In the Metabric cohort, we demonstrated that hormone therapy is more effective in patients with a low ECMI. Additionally, differentially expressed genes from the ECM-related gene list were extracted from patients with a pathologic complete response (pCR) to NAC and with residual disease (RD) to construct a neural network model for predicting the chance of achieving pCR individually. Finally, we performed qRT-PCR to validate our findings and demonstrate the important role of the gene OGN in predicting the pCR rate. In conclusion, delineation of the ECM signature with immune-related genes is anticipated to aid in the prediction of the prognosis of patients with BC and the benefits of hormone therapy and NAC in BC patients.
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http://dx.doi.org/10.3389/fimmu.2022.888339DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9331652PMC
July 2022

Ensemble Learning-Based Feature Selection for Phage Protein Prediction.

Front Microbiol 2022 15;13:932661. Epub 2022 Jul 15.

School of Computer Science and Technology, Harbin Institute of Technology, Harbin, China.

Phage has high specificity for its host recognition. As a natural enemy of bacteria, it has been used to treat super bacteria many times. Identifying phage proteins from the original sequence is very important for understanding the relationship between phage and host bacteria and developing new antimicrobial agents. However, traditional experimental methods are both expensive and time-consuming. In this study, an ensemble learning-based feature selection method is proposed to find important features for phage protein identification. The method uses four types of protein sequence-derived features, quantifies the importance of each feature by adding perturbations to the features to influence the results, and finally splices the important features among the four types of features. In addition, we analyzed the selected features and their biological significance.
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http://dx.doi.org/10.3389/fmicb.2022.932661DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9335128PMC
July 2022

CircRNA ITCH: Insight Into Its Role and Clinical Application Prospect in Tumor and Non-Tumor Diseases.

Front Genet 2022 15;13:927541. Epub 2022 Jul 15.

Department of Gastrointestinal Nutrition and Hernia Surgery, The Second Hospital of Jilin University, Changchun, China.

CircRNA E3 ubiquitin protein ligase (ITCH) (circRNA ITCH, circ-ITCH), a stable closed-loop RNA derived from the 20q11.22 region of chromosome 20, is a new circRNA discovered in the cytoplasm in recent decades. Studies have shown that it does not encode proteins, but regulates proteins expression at different levels. It is down-regulated in tumor diseases and is involved in a number of biological activities, including inhibiting cell proliferation, migration, invasion, and promoting apoptosis. It can also alter disease progression in non-tumor disease by affecting the cell cycle, inflammatory response, and critical proteins. Circ-ITCH also holds a lot of promise in terms of tumor and non-tumor clinical diagnosis, prognosis, and targeted therapy. As a result, in order to aid clinical research in the hunt for a new strategy for diagnosing and treating human diseases, this study describes the mechanism of circ-ITCH as well as its clinical implications.
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http://dx.doi.org/10.3389/fgene.2022.927541DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9335290PMC
July 2022

Metal-organic framework-derived Co/CoO nanoparticles with tunable particle size for strong low-frequency microwave absorption in the S and C bands.

J Colloid Interface Sci 2022 Jul 25;628(Pt A):10-21. Epub 2022 Jul 25.

Key Laboratory of Aerospace Materials and Performance (Ministry of Education) School of Materials Science and Engineering, Beihang University, No. 37 Xueyuan Road, Beijing 100191, PR China. Electronic address:

Nowadays, constructing strong absorption materials addressing the low-frequency electromagnetic radiation (S and C bands) from electronic devices remains a significant challenge. In this work, size-tunable Co/CoO nanoparticles (NPs) are fabricated by decomposing zeolitic imidazolate framework (ZIF-67) precursors and subsequent hydrogen reduction. All samples show obvious low-frequency attenuation in the S and C bands. At a thin thickness of 2.3 mm, the minimum reflection loss (RL) value for the Co/CoO NPs of 30 nm reaches up to -90.3 dB at 4.4 GHz, and the corresponding effective absorption bandwidth (EAB) of RL ≤ -10 dB ranges from 3.8 to 5.4 GHz. Notably, 90 % of the electromagnetic waves can be absorbed in the frequency range of 2.3-13.2 GHz, covering almost the entire S, C, and X bands at a thickness of 1.0-4.0 mm. The strong low-frequency absorption performance is attributed to the nano-porous structure, high conduction loss, tunable dielectric/magnetic loss, as well as optimized impedance matching. These Co/CoO NPs are promising candidates for high-efficient microwave absorbers in the low-frequency application.
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http://dx.doi.org/10.1016/j.jcis.2022.07.138DOI Listing
July 2022

Retraction Note: Exosome-transmitted miR-128-3p increase chemosensitivity of oxaliplatin-resistant colorectal cancer.

Mol Cancer 2022 Jul 30;21(1):157. Epub 2022 Jul 30.

Department of Clinical Laboratory, The Second Hospital of Shandong University, No. 247 Beiyuan Street, Jinan, 250033, China.

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http://dx.doi.org/10.1186/s12943-022-01627-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338635PMC
July 2022

COVID-19 vaccination and carditis in children and adolescents: a systematic review and meta-analysis.

Clin Res Cardiol 2022 Jul 30. Epub 2022 Jul 30.

Research Department of Practice and Policy, School of Pharmacy, University College London, London, UK.

Background: Coronavirus Disease-2019 (COVID-19) vaccination has been associated with the development of carditis, especially in children and adolescent males. However, the rates of these events in the global setting have not been explored in a systematic manner. The aim of this systematic review and meta-analysis is to investigate the rates of carditis in children and adolescents receiving COVID-19 vaccines.

Methods: PubMed, Embase and several Latin American databases were searched for studies. The number of events, and where available, at-risk populations were extracted. Rate ratios were calculated and expressed as a rate per million doses received. Subgroup analysis based on the dose administered was performed. Subjects ≤ 19 years old who developed pericarditis or myocarditis following COVID-19 vaccination were included.

Results: A total of 369 entries were retrieved. After screening, 39 articles were included. Our meta-analysis found that 343 patients developed carditis after the administration of 12,602,625 COVID-19 vaccination doses (pooled rate per million: 37.76; 95% confidence interval [CI] 23.57, 59.19). The rate of carditis was higher amongst male patients (pooled rate ratio: 5.04; 95% CI 1.40, 18.19) and after the second vaccination dose (pooled rate ratio: 5.60; 95% CI 1.97, 15.89). In 301 cases of carditis (281 male; mean age: 15.90 (standard deviation [SD] 1.52) years old) reported amongst the case series/reports, 261 patients were reported to have received treatment. 97.34% of the patients presented with chest pain. The common findings include ST elevation and T wave abnormalities on electrocardiography. Oedema and late gadolinium enhancement in the myocardium were frequently observed in cardiac magnetic resonance imaging (CMR). The mean length of hospital stay was 3.91 days (SD 1.75). In 298 out of 299 patients (99.67%) the carditis resolved with or without treatment.

Conclusions: Carditis is a rare complication after COVID-19 vaccination across the globe, but the vast majority of episodes are self-limiting with rapid resolution of symptoms within days. Central illustration. Balancing the benefits of vaccines on COVID-19-caused carditis and post-vaccination carditis.
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http://dx.doi.org/10.1007/s00392-022-02070-7DOI Listing
July 2022

Bacillus coagulans in Combination with Chitooligosaccharides Regulates Gut Microbiota and Ameliorates the DSS-Induced Colitis in Mice.

Microbiol Spectr 2022 Jul 28:e0064122. Epub 2022 Jul 28.

Antibiotics Research and Re-evaluation Key Laboratory of Sichuan Province, Sichuan Industrial Institute of Antibiotics, School of Pharmacy, Chengdu Universitygrid.411292.d, Chengdu, People's Republic of China.

Ulcerative colitis (UC) are chronic inflammatory disorders, which may be caused by intestinal barrier dysfunction, immune system disorders and intestinal microbiota dysbiosis. Synbiotic, the combination of probiotics and prebiotics, is thought to be a pragmatic approach in mitigating inflammation in UC. Bacillus coagulans has been recognized as a potential probiotic for treating intestinal diseases because of its favorable industrial and probiotic properties, including sporulation and lactic acid production. In this study, we evaluated the treatment effects of the FCYS01 spores with or without the chitooligosaccharides (COSs) on UC generated using dextran sulfate sodium (DSS) in mice. Supplementation of spores, prebiotic COSs or the synbiotic (the spores + COSs) had a significant positive effect on DSS-induced UC. The disease activity index and histological damage score were significantly reduced after these supplementations. Compared to DSS group, these supplementations also significantly modulated the cytokines IL-4, IL-6, IL-8, IL-10, and C-reactive protein (CRP) levels and significantly maintained expressions of tight junction proteins and mucin protein and promotes recovery of the intestinal barrier. In addition, these supplementations regulate the composition of gut microbiota and improve the production of short-chain fatty acids (SCFAs), through enrichment of SCFA-producing bacteria, such as and species. In summary, the synbiotic ameliorated the overall inflammatory status of the experimental UC model and showed a better treatment effect than or COSs did alone as revealed by the markers such as, colon length, IL-4 and Occludin levels. Probiotic and prebiotic are believed to be useful in alleviating the inflammatory, thereby resolving or preventing the severity of UC. Spore-forming bacteria Bacillus coagulans show advantages of stability and probiotic effects, being suggested as the important probiotics for UC treatment. Here, we demonstrate that administration of spores, chitooligosaccharides (COSs), or the synbiotic attenuates DSS-induced colitis and significantly correlates with altered gut immune responses. The treatment effect of the synbiotic is inferred to be relied on the enrichment of probiotic bacteria, such as and species, which are reported to be crucial important for gut health. Our findings facilitate the development of therapeutic and preventive strategies for UC using spore-forming lactic acid bacteria in combination with COSs.
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http://dx.doi.org/10.1128/spectrum.00641-22DOI Listing
July 2022

Novel Diagnostic and Prognostic Tools for Lung Cancer Cachexia: Based on Nutritional and Inflammatory Status.

Front Oncol 2022 11;12:890745. Epub 2022 Jul 11.

Department of Gastrointestinal Surgery, Beijing Shijitan Hospital, Capital Medical University, Beijing, China.

Background: Cachexia is one of the most common complications affecting lung cancer patients that seriously affects their quality-of-life and survival time. This study aimed to analyze the predictors and prognostic factors of lung cancer cachexia as well as to develop a convenient and accurate clinical prediction tool for oncologists.

Methods: In this multicenter cohort study, 4022 patients with lung cancer were retrospectively analyzed. The patients were randomly categorized into training and verification sets (7:3 ratio). Univariate and multivariate logistic regression analyses were performed to determine the risk factors of cachexia in patients with lung cancer. Cox regression analysis was applied to determine independent prognostic factors in the patients with lung cancer cachexia. Meanwhile, two nomograms were established and evaluated by time-dependent receiver operating characteristic curve, calibration curve, and decision curve analysis (DCA).

Results: Stage, serum albumin, ALI, anemia, and surgery were independent risk factors for cachexia in patients with lung cancer. Patients with lung cancer cachexia have a shorter survival time. Sex, stage, serum albumin, ALI, KPS score, and surgery served as independent prognostic factors for patients with lung cancer cachexia. The area under the curves (AUCs) of diagnostic nomogram in the training and validation sets were 0.702 and 0.688, respectively, the AUCs of prognostic nomogram in the training set for 1-, 3-, and 5-year were 0.70, 0.72, and 0.75, respectively, while in the validation set the AUCs were 0.71, 0.75, and 0.79, respectively. The calibration curves and DCA of the two nomograms were consistent and the clinical benefit rate was high.

Conclusion: Cachexia brings an additional economic burden and worsens the prognosis of lung cancer patients. The two nomograms can accurately screen and predict the probability of occurrence of cachexia in lung cancer and the prognosis of patients with lung cancer cachexia, and guide clinical work.
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http://dx.doi.org/10.3389/fonc.2022.890745DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309732PMC
July 2022

Clinical Characteristics, Genetic Findings and Arrhythmic Outcomes of Patients with Catecholaminergic Polymorphic Ventricular Tachycardia from China: A Systematic Review.

Life (Basel) 2022 Jul 22;12(8). Epub 2022 Jul 22.

Cardiac Electrophysiology Unit, Cardiovascular Analytics Group, China-UK Collaboration, Hong Kong, China.

Introduction: Catecholaminergic polymorphic ventricular tachycardia (CPVT) is a rare inherited cardiac ion channelopathy. The present study aims to examine the clinical characteristics, genetic basis, and arrhythmic outcomes of CPVT patients from China to elucidate the difference between CPVT patients in Asia and Western countries.

Methods: PubMed and Embase were systematically searched for case reports or series reporting on CPVT patients from China until 19 February 2022 using the keyword: "Catecholaminergic Polymorphic Ventricular Tachycardia" or "CPVT", with the location limited to: "China" or "Hong Kong" or "Macau" in Embase, with no language or publication-type restriction. Articles that did not state a definite diagnosis of CPVT and articles with duplicate cases found in larger cohorts were excluded. All the included publications in this review were critically appraised based on the Joanna Briggs Institute Critical Appraisal Checklist. Clinical characteristics, genetic findings, and the primary outcome of spontaneous ventricular tachycardia/ventricular fibrillation (VT/VF) were analyzed.

Results: A total of 58 unique cases from 15 studies (median presentation age: 8 (5.0-11.8) years old) were included. All patients, except one, presented at or before 19 years of age. There were 56 patients (96.6%) who were initially symptomatic. Premature ventricular complexes (PVCs) were present in 44 out of 51 patients (86.3%) and VT in 52 out of 58 patients (89.7%). Genetic tests were performed on 54 patients (93.1%) with a yield of 87%. RyR2, CASQ2, TERCL, and SCN10A mutations were found in 35 (71.4%), 12 (24.5%), 1 (0.02%) patient, and 1 patient (0.02%), respectively. There were 54 patients who were treated with beta-blockers, 8 received flecainide, 5 received amiodarone, 2 received verapamil and 2 received propafenone. Sympathectomy ( = 10), implantable cardioverter-defibrillator implantation ( = 8) and ablation ( = 1) were performed. On follow-up, 13 patients developed VT/VF.

Conclusion: This was the first systematic review of CPVT patients from China. Most patients had symptoms on initial presentation, with syncope as the presenting complaint. RyR2 mutation accounts for more than half of the CPVT cases, followed by CASQ2, TERCL and SCN10A mutations.
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http://dx.doi.org/10.3390/life12081104DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330865PMC
July 2022

ICIs-Related Cardiotoxicity in Different Types of Cancer.

J Cardiovasc Dev Dis 2022 Jun 28;9(7). Epub 2022 Jun 28.

Department of Cardiology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai 264000, China.

Immune checkpoint inhibitors (ICIs) are rapidly developing immunotherapy cancer drugs that have prolonged patient survival. However, ICIs-related cardiotoxicity has been recognized as a rare, but fatal, consequence. Although there has been extensive research based on different types of ICIs, these studies have not indicated whether cardiotoxicity is specific to a type of cancer. Therefore, we conducted a systematic review to analyze a variety of ICIs-related cardiotoxicity, focusing on different types of cancer. We found that the incidence of ICIs-related cardiac adverse events (CAEs) and common cardiotoxic manifestations vary with cancer type. This inspired us to explore the underlying mechanisms to formulate targeted clinical strategies for maintaining the cardiovascular health of cancer patients.
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http://dx.doi.org/10.3390/jcdd9070203DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9324462PMC
June 2022

Association of Visit-to-Visit Variability in Fasting Plasma Glucose with Digestive Cancer Risk.

Oxid Med Cell Longev 2022 13;2022:4530894. Epub 2022 Jul 13.

Tianjin Key Laboratory of Ionic-Molecular Function of Cardiovascular Disease, Department of Cardiology, Tianjin Institute of Cardiology, Second Hospital of Tianjin Medical University, Tianjin 300211, China.

Background And Aims: The aim of this study is to investigate the association between visit-to-visit variability in fasting plasma glucose (FPG) and the risk of digestive cancers among individuals with and without diabetes.

Methods: Using data from Kailuan cohort, a prospective population-based study, individuals who had at least two measurements of FPG between 2006 and 2012 without prior cancer were included in this study. Four indexes of variability were used, including standard deviation (SD), coefficient of variation (CV), variability independent of the mean (VIM), and average successive variability (ARV). Cox regression was used to evaluate the relationship between the quartiles of FPG variability and digestive cancers.

Results: A total of 98,161 individuals were studied. Over a mean follow-up of 9.32 ± 0.81 years, 1103 individuals developed incident digestive cancer (1.21 per 1000 person-years). Compared to the individuals in the lowest quartile, those in the highest quartile of FPG variability by SD had 38.7% higher risk of developing overall digestive cancers after adjusting for the significant confounders (hazard ratio, 1.387; 95% confidence interval, 1.160-1.659; = 0.0003). Higher FPG variability was associated with significantly higher risks of colorectal cancer (fully adjusted HR 1.432, 95% CI [1.073-1.912], = 0.015) and pancreatic cancer (fully adjusted HR 2.105, 95% CI [1.024-4.329], = 0.043), but not liver cancer (fully adjusted HR 1.427, 95% CI [0.973-2.092], = 0.069) or esophageal and gastric cancer (fully adjusted HR 1.139, 95% CI [0.776-1.670], = 0.506). Subgroup analyses showed that individuals who were younger (<65 years), male, and those without diabetes experienced a predominantly higher risk of developing digestive cancers. Similar results were observed when using CV, VIM, and ARV.

Conclusions: FPG variability was significantly associated with increasing risk of digestive cancers, especially for pancreatic and colorectal cancer. Our study suggested a potential role of FPG variability in risk stratification of digestive cancers. Approaches that reduce FPG variability may lower the risks of incident digestive cancers among the general population. This trial is registered with ChiCTR-TNRC-11001489.
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http://dx.doi.org/10.1155/2022/4530894DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301759PMC
July 2022

Development of an Electronic Frailty Index for Predicting Mortality and Complications Analysis in Pulmonary Hypertension Using Random Survival Forest Model.

Front Cardiovasc Med 2022 8;9:735906. Epub 2022 Jul 8.

School of Data Science, City University of Hong Kong, Hong Kong, Hong Kong SAR, China.

Background: The long-term prognosis of the cardio-metabolic and renal complications, in addition to mortality in patients with newly diagnosed pulmonary hypertension, are unclear. This study aims to develop a scalable predictive model in the form of an electronic frailty index (eFI) to predict different adverse outcomes.

Methods: This was a population-based cohort study of patients diagnosed with pulmonary hypertension between January 1st, 2000 and December 31st, 2017, in Hong Kong public hospitals. The primary outcomes were mortality, cardiovascular complications, renal diseases, and diabetes mellitus. The univariable and multivariable Cox regression analyses were applied to identify the significant risk factors, which were fed into the non-parametric random survival forest (RSF) model to develop an eFI.

Results: A total of 2,560 patients with a mean age of 63.4 years old (interquartile range: 38.0-79.0) were included. Over a follow-up, 1,347 died and 1,878, 437, and 684 patients developed cardiovascular complications, diabetes mellitus, and renal disease, respectively. The RSF-model-identified age, average readmission, anti-hypertensive drugs, cumulative length of stay, and total bilirubin were among the most important risk factors for predicting mortality. Pair-wise interactions of factors including diagnosis age, average readmission interval, and cumulative hospital stay were also crucial for the mortality prediction. Patients who developed all-cause mortality had higher values of the eFI compared to those who survived ( < 0.0001). An eFI ≥ 9.5 was associated with increased risks of mortality [hazard ratio (HR): 1.90; 95% confidence interval [CI]: 1.70-2.12; < 0.0001]. The cumulative hazards were higher among patients who were 65 years old or above with eFI ≥ 9.5. Using the same cut-off point, the eFI predicted a long-term mortality over 10 years (HR: 1.71; 95% CI: 1.53-1.90; < 0.0001). Compared to the multivariable Cox regression, the precision, recall, area under the curve (AUC), and C-index were significantly higher for RSF in the prediction of outcomes.

Conclusion: The RSF models identified the novel risk factors and interactions for the development of complications and mortality. The eFI constructed by RSF accurately predicts the complications and mortality of patients with pulmonary hypertension, especially among the elderly.
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http://dx.doi.org/10.3389/fcvm.2022.735906DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304657PMC
July 2022

Gender-specific clinical risk scores incorporating blood pressure variability for predicting incident dementia.

J Am Med Inform Assoc 2022 Jul 23. Epub 2022 Jul 23.

Family Medicine Research Unit, Cardiovascular Analytics Group, Hong Kong, China.

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http://dx.doi.org/10.1093/jamia/ocac117DOI Listing
July 2022

Application value of contrast-enhanced ultrasound in the diagnosis of peripheral pulmonary focal lesions.

Medicine (Baltimore) 2022 Jul 22;101(29):e29605. Epub 2022 Jul 22.

Second Department of Pulmonary and Critical Care Medicine.

Introduction: Peripheral pulmonary lesions are encountered frequently in clinical practice. Accurate diagnosis of these lesions is of great importance for clinicians. Ultrasound-guided lung tissue puncture is a reliable method for diagnosing these lesions.

Objectives: To investigate the application value of contrast-enhanced ultrasound (CEUS) combined with rapid on-site evaluation (ROSE) in the diagnosis of peripheral pulmonary focal lesions.

Methods: Eighty patients enrolled from July 2020 to June 2021 were divided into two groups: a conventional ultrasound group and a CEUS group. Both groups underwent diagnostic procedures guided by ROSE to improve the success rate of puncture sampling. The success rates and complications in both groups were compared. The results for lesion enhancement, time taken for the contrast agent to reach the lesions (AT) and lung tissues (L-AT), and the difference between these times (∆AT) were compared in the CEUS group.

Results: The success rate of biopsy in the CEUS group was 97.62%, which was significantly higher than that in the conventional ultrasound group (84%; P < .05). Puncture complications did not occur in the CEUS group and occurred in 5.26% of the cases in the conventional ultrasound group, but the difference was not statistically significant (P > .05). A comparison of enhancement of benign lesions and malignant lesions in the CEUS group showed a statistically significant difference (P < .05). The difference between the AT and ∆AT of benign and malignant lesions was statistically significant (P <.05). The optimal threshold of ∆AT was 2.05 s.

Conclusion: CEUS combined with ROSE is a very important approach for biopsy in the diagnosis of peripheral pulmonary focal lesions. CEUS has definite clinical value in the diagnosis of benign and malignant lung lesions.
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http://dx.doi.org/10.1097/MD.0000000000029605DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302248PMC
July 2022

CD40×HER2 bispecific antibody overcomes the CCL2-induced trastuzumab resistance in HER2-positive gastric cancer.

J Immunother Cancer 2022 Jul;10(7)

Department of General Surgery, Tianjin Medical University General Hospital, Tianjin, China

Background: There was much hard work to study the trastuzumab resistance in HER2-positive gastric cancer (GC), but the information which would reveal this abstruse mechanism is little. In this study, we aimed to investigate the roles of tumor cell-derived CCL2 on trastuzumab resistance and overcome the resistance by treatment with the anti-CD40-scFv-linked anti-HER2 (CD40 ×HER2) bispecific antibody (bsAb).

Methods: We measured the levels of CCL2 expression in HER2-positive GC tissues, and revealed biological functions of tumor cell-derived CCL2 on tumor-associated macrophages (TAMs) and the trastuzumab resistance. Then, we developed CD40 ×HER2 bsAb, and examined the targeting roles on HER2 and CD40, to overcome the trastuzumab resistance without systemic toxicity.

Results: We found the level of CCL2 expression in HER2-postive GC was correlated with infiltration of TAMs, polarization status of infiltrated TAMs, trastuzumab resistance and survival outcomes of GC patients. On exposure to CCL2, TAMs decreased the M1-like phenotype, thereby eliciting the trastuzumab resistance. CCL2 activated the transcription of ZC3H12A, which increased K63-linked deubiquitination and K48-linked auto-ubiquitination of TRAF6/3 to inactivate NF-κB signaling in TAMs. CD40 ×HER2 bsAb, which targeted the CD40 to restore the ubiquitination level of TRAF6/3, increased the M1-like phenotypic transformation of TAMs, and overcame trastuzumab resistance without immune-related adversary effects (irAEs).

Conclusions: We revealed a novel mechanism of trastuzumab resistance in HER2-positive GC via the CCL2-ZC3H12A-TRAF6/3 signaling axis, and presented a CD40 ×HER2 bsAb which showed great antitumor efficacy with few irAEs.
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http://dx.doi.org/10.1136/jitc-2022-005063DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9295658PMC
July 2022

Expression and activation of nuclear hormone receptors result in neuronal differentiation and favorable prognosis in neuroblastoma.

J Exp Clin Cancer Res 2022 Jul 19;41(1):226. Epub 2022 Jul 19.

Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, SE-171 65, Stockholm, Sweden.

Background: Neuroblastoma (NB), a childhood tumor derived from the sympathetic nervous system, presents with heterogeneous clinical behavior. While some tumors regress spontaneously without medical intervention, others are resistant to therapy, associated with an aggressive phenotype. MYCN-amplification, frequently occurring in high-risk NB, is correlated with an undifferentiated phenotype and poor prognosis. Differentiation induction has been proposed as a therapeutic approach for high-risk NB. We have previously shown that MYCN maintains an undifferentiated state via regulation of the miR-17 ~ 92 microRNA cluster, repressing the nuclear hormone receptors (NHRs) estrogen receptor alpha (ERα) and the glucocorticoid receptor (GR).

Methods: Cell viability was determined by WST-1. Expression of differentiation markers was analyzed by Western blot, RT-qPCR, and immunofluorescence analysis. Metabolic phenotypes were studied using Agilent Extracellular Flux Analyzer, and accumulation of lipid droplets by Nile Red staining. Expression of angiogenesis, proliferation, and neuronal differentiation markers, and tumor sections were assessed by immunohistochemistry. Gene expression from NB patient as well as adrenal gland cohorts were analyzed using GraphPad Prism software (v.8) and GSEA (v4.0.3), while pseudo-time progression on post-natal adrenal gland cells from single-nuclei transcriptome data was computed using scVelo.

Results: Here, we show that simultaneous activation of GR and ERα potentiated induction of neuronal differentiation, reduced NB cell viability in vitro, and decreased tumor burden in vivo. This was accompanied by a metabolic reprogramming manifested by changes in the glycolytic and mitochondrial functions and in lipid droplet accumulation. Activation of the retinoic acid receptor alpha (RARα) with all-trans retinoic acid (ATRA) further enhanced the differentiated phenotype as well as the metabolic switch. Single-cell nuclei transcriptome analysis of human adrenal glands indicated a sequential expression of ERα, GR, and RARα during development from progenitor to differentiated chromaffin cells. Further, in silico analysis revealed that patients with higher combined expression of GR, ERα, and RARα mRNA levels had elevated expression of neuronal differentiation markers and a favorable outcome.

Conclusion: Together, our findings suggest that combination therapy involving activation of several NHRs could be a promising pharmacological approach for differentiation treatment of NB patients.
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http://dx.doi.org/10.1186/s13046-022-02399-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9295514PMC
July 2022

Predicting residue-specific qualities of individual protein models using residual neural networks and graph neural networks.

Proteins 2022 Jul 17. Epub 2022 Jul 17.

Department of Computer Science, University of Miami, Coral Gables, Florida, USA.

The estimation of protein model accuracy (EMA) or model quality assessment (QA) is important for protein structure prediction. An accurate EMA algorithm can guide the refinement of models or pick the best model or best parts of models from a pool of predicted tertiary structures. We developed two novel methods: MASS2 and LAW, for predicting residue-specific or local qualities of individual models, which incorporate residual neural networks and graph neural networks, respectively. These two methods use similar features extracted from protein models but different architectures of neural networks to predict the local accuracies of single models. MASS2 and LAW participated in the QA category of CASP14, and according to our evaluations based on CASP14 official criteria, MASS2 and LAW are the best and second-best methods based on the Z-scores of ASE/100, AUC, and ULR-1.F1. We also evaluated MASS2, LAW, and the residue-specific predicted deviations (between model and native structure) generated by AlphaFold2 on CASP14 AlphaFold2 tertiary structure (TS) models. LAW achieved comparable or better performances compared to the predicted deviations generated by AlphaFold2 on AlphaFold2 TS models, even though LAW was not trained on any AlphaFold2 TS models. Specifically, LAW performed better on AUC and ULR scores, and AlphaFold2 performed better on ASE scores. This means that AlphaFold2 is better at predicting deviations, but LAW is better at classifying accurate and inaccurate residues and detecting unreliable local regions. MASS2 and LAW can be freely accessed from http://dna.cs.miami.edu/MASS2-CASP14/ and http://dna.cs.miami.edu/LAW-CASP14/, respectively.
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http://dx.doi.org/10.1002/prot.26400DOI Listing
July 2022

The advanced lung cancer inflammation index is the optimal inflammatory biomarker of overall survival in patients with lung cancer.

J Cachexia Sarcopenia Muscle 2022 Jul 14. Epub 2022 Jul 14.

Department of Gastrointestinal Surgery/Clinical Nutrition, Capital Medical University Affiliated Beijing Shijitan Hospital, Beijing, China.

Backgrounds: Malnutrition and systemic inflammatory responses are associated with poor overall survival (OS) in lung cancer patients, but it remains unclear which biomarkers are better for predicting their prognosis. This study tried to determine the best one among the existing common nutrition/inflammation-based indicators of OS for patients with lung cancer.

Materials And Methods: There were 16 nutrition or systemic inflammation-based indicators included in this study. The cut-off points for the indicators were calculated using maximally selected rank statistics. The OS was evaluated using the Kaplan-Meier estimator, and univariate and multivariate Cox proportional hazard models were used to determine the relationship between the indicators and OS. A time-dependent receiver operating characteristic curves (time-ROC) and C-index were calculated to assess the predictive ability of the different indicators.

Results: There were 1772 patients with lung cancer included in this study. In univariate analysis, all 16 indicators were significantly associated with OS of the patients (all P < 0.001). Except for platelet-to-lymphocyte ratio, all other indicators were independent predictors of OS in multivariate analysis (all P < 0.05). Low advanced lung cancer inflammation index (ALI) was associated with higher mortality risk of lung cancer [hazard ratio, 1.30; 95% confidence interval (CI), 1.13-1.49]. The results of the time-AUC and C-index analyses indicated that the ALI (C-index: 0.611) had the best predictive ability on the OS in patients with lung cancer. In different sub-groups, the ALI was the best indicator for predicting the OS of lung cancer patients regardless of sex (C-index, 0.609 for men and 0.613 for women) or smoking status (C-index, 0.629 for non-smoker and 0.601 for smoker) and in patients aged <65 years (C-index, 0.613). However, the modified Glasgow prognostic score was superior to the other indicators in non-small cell lung cancer patients (C-index, 0.639) or patients aged ≥65 years (C-index, 0.610), and the glucose-to-lymphocyte ratio performed better prognostic ability in patients with small cell lung cancer (C-index, 0.601).

Conclusions: The prognostic ability of the ALI is superior to the other inflammation/nutrition-based indicators for all patients with lung cancer.
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http://dx.doi.org/10.1002/jcsm.13032DOI Listing
July 2022

Low Serum Dehydroepiandrosterone and Dehydroepiandrosterone Sulfate Are Associated With Coronary Heart Disease in Men With Type 2 Diabetes Mellitus.

Front Endocrinol (Lausanne) 2022 27;13:890029. Epub 2022 Jun 27.

Department of Endocrinology and Metabolism, Tianjin Medical University General Hospital, Tianjin, China.

Aims: Sex hormones play an important role in the pathogenesis of cardiovascular disease (CVD). This cross-sectional study aimed to explore the associations of dehydroepiandrosterone (DHEA) and dehydroepiandrosterone sulfate (DHEAS) with coronary heart disease (CHD) and stroke in middle-aged and elderly patients with type 2 diabetes mellitus (T2DM).

Materials And Methods: A total of 995 patients with T2DM were included in the study analysis. Serum levels of DHEA and DHEAS were quantified using liquid chromatography-tandem mass spectrometry. Binary logistic regression analyses were performed to assess the associations of DHEA and DHEAS with CHD and stroke. Receiver operating characteristic (ROC) curve analysis was performed to determine the optimal DHEA and DHEAS cutoff values for the detection of CHD in men with T2DM.

Results: In men with T2DM, after adjustment for potential confounders in model 3, the risk of CHD decreased with an increasing serum DHEA level [odds ratio (OR) = 0.38, quartile 4 . quartile 1; 95% confidence interval (CI) = 0.16-0.90;  = 0.037 for trend). Consistently, when considered as a continuous variable, this association remained significant in the fully adjusted model (OR = 0.59, 95% CI = 0.40-0.87,  < 0.05). When taken as a continuous variable in model 3, serum DHEAS level was also inversely related to the risk of CHD among men (OR = 0.56, 95% CI = 0.38-0.82,  < 0.05). Similarly, this relationship remained statistically significant when DHEAS was categorized into quartiles (OR = 0.27, quartile 4 . quartile 1; 95% CI = 0.11-0.67;  = 0.018 for trend). ROC curve analyses revealed that the optimal cutoff values to detect CHD in men with T2DM were 6.43 nmol/L for DHEA and 3.54 μmol/L for DHEAS. In contrast, no significant associations were found between DHEA and DHEAS on the one hand and stroke on the other in men and women with T2DM (all  > 0.05).

Conclusions: Serum DHEA and DHEAS were significantly and negatively associated with CHD in middle-aged and elderly men with T2DM. This study suggests potential roles of DHEA and DHEAS in CHD pathogenesis.
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http://dx.doi.org/10.3389/fendo.2022.890029DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9271610PMC
June 2022

Quantitative proteomics analysis to assess protein expression levels in the ovaries of pubescent goats.

BMC Genomics 2022 Jul 13;23(1):507. Epub 2022 Jul 13.

Department of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, Anhui, China.

Background: Changes in the abundance of ovarian proteins play a key role in the regulation of reproduction. However, to date, no studies have investigated such changes in pubescent goats. Herein we applied isobaric tags for relative and absolute quantitation (iTRAQ) and liquid chromatography-tandem mass spectrometry to analyze the expression levels of ovarian proteins in pre-pubertal (n = 3) and pubertal (n = 3) goats.

Results: Overall, 7,550 proteins were recognized; 301 (176 up- and 125 downregulated) were identified as differentially abundant proteins (DAPs). Five DAPs were randomly selected for expression level validation by Western blotting; the results of Western blotting and iTRAQ analysis were consistent. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis indicated that DAPs were enriched in olfactory transduction, glutathione metabolism, and calcium signaling pathways. Besides, gene ontology functional enrichment analysis revealed that several DAPs enriched in biological processes were associated with cellular process, biological regulation, metabolic process, and response to stimulus. Protein-protein interaction network showed that proteins interacting with CDK1, HSPA1A, and UCK2 were the most abundant.

Conclusions: We identified 301 DAPs, which were enriched in olfactory transduction, glutathione metabolism, and calcium signaling pathways, suggesting the involvement of these processes in the onset of puberty. Further studies are warranted to more comprehensively explore the function of the identified DAPs and aforementioned signaling pathways to gain novel, deeper insights into the mechanisms underlying the onset of puberty.
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http://dx.doi.org/10.1186/s12864-022-08699-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9281040PMC
July 2022

Molecular Design, Supramolecular Assembly, and Excellent Dye Adsorption Capacity of Natural Rigid Dehydroabietic Acid-Tailored Amide Organogelators.

Langmuir 2022 Jul 12;38(29):8918-8927. Epub 2022 Jul 12.

MOE Engineering Research Center of Forestry Biomass Materials and Bioenergy, College of Materials Science and Technology, Beijing Forestry University, Beijing 100083, China.

It is very appealing to synthesize functional soft materials from natural and abundant plant diterpenes because they have conformationally rigid and chiral properties. Herein, dehydroabietic-based monoamide (DA-1) and diamide (DA-2) were designed by introducing device interactions, π-π stacking and hydrogen bonding, with an aromatic group, C═O, and N-H. DA-1 and DA-2 can be gelled in a mixed solvent and a single solvent, respectively. Several novel supramolecular organic gels including highly entangled three-dimensional networks composed of rods or fibers were constructed. Interestingly, DA-2 forms a helical structure that is right-handed under the cooperative control of the solvent and the rigid structure of rosin. Gel formation was primarily driven by hydrogen bonding, π-π stacking, and van der Waals force. Combined with Gaussian calculation and X-ray diffraction (XRD), we established pack patterns for each system, revealing the roles played by rosin and amide groups. Moreover, the carbon tetrachloride gel of DA-2 can effectively remove Congo red in an aqueous solution, and the removal rate can reach 98.4%. This research explores an efficient organic gel for adsorbing Congo red dye with the secretions of pine trees.
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http://dx.doi.org/10.1021/acs.langmuir.2c01068DOI Listing
July 2022

Efficient Excitation and Tuning of Multi-Fano Resonances with High Q-Factor in All-Dielectric Metasurfaces.

Nanomaterials (Basel) 2022 Jul 4;12(13). Epub 2022 Jul 4.

College of Science, Xi'an University of Science and Technology, Xi'an 710054, China.

Exciting Fano resonance can improve the quality factor (Q-factor) and enhance the light energy utilization rate of optical devices. However, due to the large inherent loss of metals and the limitation of phase matching, traditional optical devices based on surface plasmon resonance cannot obtain a larger Q-factor. In this study, a silicon square-hole nano disk (SHND) array device is proposed and studied numerically. The results show that, by breaking the symmetry of the SHND structure and transforming an ideal bound state in the continuum (BIC) with an infinite Q-factor into a quasi-BIC with a finite Q-factor, three Fano resonances can be realized. The calculation results also show that the three Fano resonances with narrow linewidth can produce significant local electric and magnetic field enhancements: the highest Q-factor value reaches 35,837, and the modulation depth of those Fano resonances can reach almost 100%. Considering these properties, the SHND structure realizes multi-Fano resonances with a high Q-factor, narrow line width, large modulation depth and high near-field enhancement, which could provide a new method for applications such as multi-wavelength communications, lasing, and nonlinear optical devices.
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http://dx.doi.org/10.3390/nano12132292DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9268095PMC
July 2022

Enhanced internal field of BiNbOCl ferroelectric photocatalyst to promote charge separation via constructing crystal plane-dependent BiOI dielectric layer.

J Colloid Interface Sci 2022 Jun 30;626:405-415. Epub 2022 Jun 30.

State Key Laboratory of Optoelectronic Materials and Technologies, School of Materials Science and Engineering, Sun Yat-Sen University, Guangzhou 510275, PR China. Electronic address:

Spontaneous polarization induced by the unique crystal structure of ferroelectric semiconductor photocatalyst facilitates charge separation and injects new vitality into the improvement of the photocatalytic activity. However, due to the complexity of multi-electric field coupling, the actual efficiency of charge separation driven by the depolarization field is restricted by the shielding field, which is lower than theoretical expectations. Here, we take BiNbOCl as a model system and selectively construct a BiOI dielectric layer on its positive polarized surface through the adsorption-self-assembly method, aiming to reduce the attenuation of the shielding field to the depolarization field. The enhanced residual depolarization field (RDF) is quantitatively characterized by ferroelectric performance test. Moreover, the charge transfer path and final position are elaborated by photo-deposition experiments, while high-quality interface and calculated difference of the potential between BiNbOCl and BiOI is responsible for the formation of charge transfer channel. The enhanced RDF promotes the separation of charges, which causes that BiNbOCl/BiOI photo-degradation of bisphenol A (BPA) gives 7.35-fold greater efficiency than BiNbOCl. This scheme of weakening the shielding field by surface reconstruction engineering is promising to be extended to more ferroelectric photocatalyst systems.
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http://dx.doi.org/10.1016/j.jcis.2022.06.136DOI Listing
June 2022
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