Publications by authors named "Tong Li"

1,589 Publications

  • Page 1 of 1

Explainable Artificial Intelligence Methods in Combating Pandemics: A Systematic Review.

IEEE Rev Biomed Eng 2022 Jun 23;PP. Epub 2022 Jun 23.

Despite the myriad peer-reviewed papers demonstrating novel Artificial Intelligence (AI)-based solutions to COVID-19 challenges during the pandemic, few have made a significant clinical impact, especially in diagnosis and disease precision staging. One major cause for such low impact is the lack of model transparency, significantly limiting the AI adoption in real clinical practice. To solve this problem, AI models need to be explained to users. Thus, we have conducted a comprehensive study of Explainable Artificial Intelligence (XAI) using PRISMA technology. Our findings suggest that XAI can improve model performance, instill trust in the users, and assist users in decision-making. In this systematic review, we introduce common XAI techniques and their utility with specific examples of their application. We discuss the evaluation of XAI results because it is an important step for maximizing the value of AI-based clinical decision support systems. Additionally, we present the traditional, modern, and advanced XAI models to demonstrate the evolution of novel techniques. Finally, we provide a best practice guideline that developers can refer to during the model experimentation. We also offer potential solutions with specific examples for common challenges in AI model experimentation. This comprehensive review, hopefully, can promote AI adoption in biomedicine and healthcare.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1109/RBME.2022.3185953DOI Listing
June 2022

The effect of discrete wavelengths of visible light on the developing murine embryo.

J Assist Reprod Genet 2022 Jun 23. Epub 2022 Jun 23.

School of Biomedicine, Robinson Research Institute, The University of Adelaide, Adelaide, SA, 5005, Australia.

Purpose: A current focus of the IVF field is non-invasive imaging of the embryo to quantify developmental potential. Such approaches use varying wavelengths to gain maximum biological information. The impact of irradiating the developing embryo with discrete wavelengths of light is not fully understood. Here, we assess the impact of a range of wavelengths on the developing embryo.

Methods: Murine preimplantation embryos were exposed daily to wavelengths within the blue, green, yellow, and red spectral bands and compared to an unexposed control group. Development to blastocyst, DNA damage, and cell number/allocation to blastocyst cell lineages were assessed. For the longer wavelengths (yellow and red), pregnancy/fetal outcomes and the abundance of intracellular lipid were investigated.

Results: Significantly fewer embryos developed to the blastocyst stage when exposed to the yellow wavelength. Elevated DNA damage was observed within embryos exposed to blue, green, or red wavelengths. There was no effect on blastocyst cell number/lineage allocation for all wavelengths except red, where there was a significant decrease in total cell number. Pregnancy rate was significantly reduced when embryos were irradiated with the red wavelength. Weight at weaning was significantly higher when embryos were exposed to yellow or red wavelengths. Lipid abundance was significantly elevated following exposure to the yellow wavelength.

Conclusion: Our results demonstrate that the impact of light is wavelength-specific, with longer wavelengths also impacting the embryo. We also show that effects are energy-dependent. This data shows that damage is multifaceted and developmental rate alone may not fully reflect the impact of light exposure.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10815-022-02555-4DOI Listing
June 2022

Ionization of D571 Is Coupled with SARS-CoV-2 Spike Up/Down Equilibrium Revealing the pH-Dependent Allosteric Mechanism of Receptor-Binding Domains.

J Phys Chem B 2022 Jun 23. Epub 2022 Jun 23.

Shanghai Engineering Research Center of Molecular Therapeutics and New Drug Development, Shanghai Frontiers Science Center of Molecule Intelligent Syntheses, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200062, China.

As a type I viral fusion protein, SARS-CoV-2 spike undergoes a pH-dependent switch to mediate the endosomal positioning of the receptor-binding domain to facilitate viral entry into cells and immune evasion. Gaps in our knowledge concerning the conformational transitions and key intramolecular motivations have hampered the development of effective therapeutics against the virus. To clarify the pH-sensitive elements on spike-gating the receptor-binding domain (RBD) opening and understand the details of the RBD opening transition, we performed microsecond-time scale constant pH molecular dynamics simulations in this study. We identified the deeply buried D571 with a clear p shift, suggesting a potential pH sensor, and showed the coupling of ionization of D571 with spike RBD-up/down equilibrium. We also computed the free-energy landscape for RBD opening and identified the crucial interactions that influence RBD dynamics. The atomic-level characterization of the pH-dependent spike activation mechanism provided herein offers new insights for a better understanding of the fundamental mechanisms of SARS-CoV-2 viral entry and infection and hence supports the discovery of novel therapeutics for COVID-19.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jpcb.2c02365DOI Listing
June 2022

Youssoufenes A2 and A3, Antibiotic Dimeric Cinnamoyl Lipids from the Δ Mutant of a Marine-Derived Strain.

Mar Drugs 2022 Jun 15;20(6). Epub 2022 Jun 15.

Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.

Two new dimeric cinnamoyl lipids (CL) featuring with an unusual dearomatic carbon-bridge, named youssoufenes A2 () and A3 (), were isolated from the Δ mutant strain of marine-derived OUC6819. Structures of the isolated compounds were elucidated based on extensive MS and NMR spectroscopic analyses, and their absolute configurations were determined by combination of the long-range NOE-based H-H distance measurements and ECD calculations. Compounds and exhibited moderate growth inhibition against multi-drug-resistant CCARM 5172 with an MIC value of 22.2 μM.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/md20060394DOI Listing
June 2022

Calorie restriction on normal body weight mice prevents body weight regain on a follow-up high-fat diet by shaping an obesity-resistant-like gut microbiota profile.

Food Funct 2022 Jun 23. Epub 2022 Jun 23.

Department of Nutrition and Food Safety, School of Public Health, Fujian Medical University, FuZhou 350122, P.R. China.

Calorie restriction (CR) is one of the most common approaches for obesity treatment, but whether resuming feeding after CR in normal-weight mice can affect excessive weight regain remains poorly studied. To address this issue, male C57BL/6 mice were placed in three groups: a control group ( = 10), a group fed normal diet with 30% CR ( = 20); and a group fed a HF diet ( = 30). After four weeks, the CR group was fed either a normal diet (NDCR, = 10) or a high-fat diet (HFCR, = 10) for an additional eight weeks. At the end of the experiment, mice in the HF group ranked in the upper and lower thirds for weight gain were designated as obesity-prone (HFOP, = 10) and obesity-resistant (HFOR, = 10), respectively. CR delayed weight regain and visceral fat accumulation. Gut microbiota in the HFCR group were more similar to the HFOR group than the HFOP group, mainly due to reversion of the decreased level of Clostridiales induced by CR. Mediation analysis showed that Clostridiales may delay body weight regain by affecting the interconversion of succinate and fumarate. Random forest and structural equation analyses showed Christensenellaceae were the most important biomarker for alleviation of obesity. In conclusion, CR shapes an obesity-resistant-like gut microbiota profile that may attenuate body weight regain.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1fo04358gDOI Listing
June 2022

Human Plasma Transcriptome Implicates Dysregulated S100A12 Expression: A Strong, Early-Stage Prognostic Factor in ST-Segment Elevated Myocardial Infarction: Bioinformatics Analysis and Experimental Verification.

Front Cardiovasc Med 2022 1;9:874436. Epub 2022 Jun 1.

Department of Heart Center, The Tianjin Third Central Hospital, Tianjin, China.

The ability of blood transcriptome analysis to identify dysregulated pathways and outcome-related genes following myocardial infarction remains unknown. Two gene expression datasets (GSE60993 and GSE61144) were downloaded from Gene Expression Omnibus (GEO) Datasets to identify altered plasma transcriptomes in patients with ST-segment elevated myocardial infarction (STEMI) undergoing primary percutaneous coronary intervention. GEO2R, Gene Ontology/Kyoto Encyclopedia of Genes and Genomes annotations, protein-protein interaction analysis, etc., were adopted to determine functional roles and regulatory networks of differentially expressed genes (DEGs). Dysregulated expressomes were verified at transcriptional and translational levels by analyzing the GSE49925 dataset and our own samples, respectively. A total of 91 DEGs were identified in the discovery phase, consisting of 15 downregulated genes and 76 upregulated genes. Two hub modules consisting of 12 hub genes were identified. In the verification phase, six of the 12 hub genes exhibited the same variation patterns at the transcriptional level in the GSE49925 dataset. Among them, S100A12 was shown to have the best discriminative performance for predicting in-hospital mortality and to be the only independent predictor of death during follow-up. Validation of 223 samples from our center showed that S100A12 protein level in plasma was significantly lower among patients who survived to discharge, but it was not an independent predictor of survival to discharge or recurrent major adverse cardiovascular events after discharge. In conclusion, the dysregulated expression of plasma S100A12 at the transcriptional level is a robust early prognostic factor in patients with STEMI, while the discrimination power of the protein level in plasma needs to be further verified by large-scale, prospective, international, multicenter studies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcvm.2022.874436DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9200219PMC
June 2022

SAST-GCN: Segmentation Adaptive Spatial Temporal-Graph Convolutional Network for P3-Based Video Target Detection.

Front Neurosci 2022 2;16:913027. Epub 2022 Jun 2.

Henan Key Laboratory of Imaging and Intelligent Processing, People's Liberation Army of China (PLA) Strategic Support Force Information Engineering University, Zhengzhou, China.

Detecting video-induced P3 is crucial to building the video target detection system based on the brain-computer interface. However, studies have shown that the brain response patterns corresponding to video-induced P3 are dynamic and determined by the interaction of multiple brain regions. This paper proposes a segmentation adaptive spatial-temporal graph convolutional network (SAST-GCN) for P3-based video target detection. To make full use of the dynamic characteristics of the P3 signal data, the data is segmented according to the processing stages of the video-induced P3, and the brain network connections are constructed correspondingly. Then, the spatial-temporal feature of EEG data is extracted by adaptive spatial-temporal graph convolution to discriminate the target and non-target in the video. Especially, a style-based recalibration module is added to select feature maps with higher contributions and increase the feature extraction ability of the network. The experimental results demonstrate the superiority of our proposed model over the baseline methods. Also, the ablation experiments indicate that the segmentation of data to construct the brain connection can effectively improve the recognition performance by reflecting the dynamic connection relationship between EEG channels more accurately.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fnins.2022.913027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201684PMC
June 2022

A Study on the Influence of Authoritarian-Benevolent Leadership on Employees' Innovative Behavior From the Perspective of Psychological Perception-Based on Fuzzy Set Qualitative Comparative Analysis.

Front Psychol 2022 2;13:886286. Epub 2022 Jun 2.

Yunnan College of Tourism Vocation, Kunming, China.

Employee innovation is the key to enhancing the core competitiveness of an enterprise, and leadership style plays an important role in stimulating employees' innovative behavior. This study explores the impact of unique ambidextrous leadership in the Chinese context, authoritarian-benevolent leadership, on employees' innovative behavior from the perspective of employees' psychological perception, based on research data from 430 employees of companies with direct leaders. Based on the configuration theory, using the fuzzy set qualitative comparative analysis method, the configuration analysis was carried out by taking authoritarian-benevolent ambidextrous leadership and employees' psychological perception as the influencing factors and obtained five configurations of high-level employees' innovative behavior. The results show that the combination of individualized care, understanding, and forgiving of benevolent leadership and Shang-yan of authoritarian leadership can effectively stimulate employees' innovative behavior. Juan-Chiuan leadership is not conducive to employees' innovative behavior. Employees' high psychological safety and low uncertainty are important conditions for promoting employee innovation. In this study, the four dimensions of authoritarian-benevolent leadership and the psychological perceptions of employees are discussed in combination, and the paths of motivating employees to innovate actively are obtained. It is hoped that it can provide certain ideas for leaders to promote employee innovation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpsyg.2022.886286DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201952PMC
June 2022

[Development and validation of a predictive model for the risk of 30-day death in emergency department patients].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2022 Apr;34(4):421-425

Department of Nursing, the First People's Hospital of Changde, Changde 415000, Hunan, China. Corresponding author: Tong Li, Email:

Objective: To explore the risk factors for 30-day death in emergency department patients, and then construct a prediction model and validate it using nomogram.

Methods: A retrospective cohort study was conducted. The clinical data of 1 091 patients admitted to the emergency department of the First People's Hospital of Changde from January 1 to June 30, 2021 was collected, including 741 patients from January 1 to March 31 in the development group and 350 patients from April 1 to June 30 in the validation group. General information, first vital signs admitted to the emergency department, and laboratory results were collected, the modified early warning score (MEWS) was calculated, and 30-day outcomes were recorded. Univariate and multivariate Logistic regression analysis was used to screen out the risk factors of 30-day death. According to the results of multivariate analysis, the nomogram was used to construct a 30-day death prediction model. The receiver operator characteristic curve (ROC curve) was used to evaluate the consistency of the prediction model, the calibration of the prediction model was evaluated by the Hosmer-Lemeshow goodness of fit test.

Results: A total of 1 091 patients were enrolled. There were 741 patients in the development group, including 356 males and 385 females, aged (51.42±17.33) years old, and the 30-day mortality was 28.88%. There were 350 patients in the validation group, including 188 males and 162 females, aged (52.88±16.11) years old, and the 30-day mortality was 24.00%. The results of the univariate analysis showed that age, primary diagnosis on admission, consciousness, respiratory rate (RR), systolic blood pressure (SBP), heart rate (HR), pulse oxygen saturation (SpO), MEWS score, erythrocyte sedimentation rate (ESR), procalcitonin (PCT) and body mass index (BMI) might be the risk factors for 30-day death in patients in the emergency department. The results of the multivariate analysis showed that the MEWS score [odds ratio (OR) = 14.22, 95% confidence interval (95%CI) was 1.46-138.12], ESR (OR = 46.71, 95%CI was 20.48-106.53), PCT (OR = 4.97, 95%CI was 2.46-10.02), BMI (24.0-27.9 kg/m: OR = 37.82, 95%CI was 14.69-97.36; ≥ 28.0 kg/m: OR = 62.11, 95%CI was 25.77-149.72) were independent risk factors for 30-day death in the emergency department (all P < 0.05). Using the four variables with the results of multivariate analysis to construct a nomogram prediction model, the area under the ROC curve (AUC) was 0.974 (95%CI was 0.753-0.983) for the development group, and the AUC was 0.963 (95%CI was 0.740-0.975) for the validation group. The Hosmer-Lemeshow test showed no statistically significant difference between the predicted outcome of the nomogram prediction model and the actual occurrence (χ = 1.216, P = 1.270).

Conclusion: The prediction model developed by the MEWS score combined with BMI, ESR and PCT can scientifically and effectively predict the 30-day outcome of emergency department patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3760/cma.j.cn121430-20210830-01291DOI Listing
April 2022

DAF-16 is involved in colonic metabolites of Ferulic acid promoted the longevity and stress resistance of C.elegans.

J Sci Food Agric 2022 Jun 11. Epub 2022 Jun 11.

Department of Food Science, Cornell University, Ithaca, New York, 14853, US.

Background: Ferulic acid (FA) is a dietary polyphenol widely found in plant tissues. It has long been considered to have health-promoting qualities. However, the biological properties of dietary polyphenols depend largely on their absorption during digestion, and the effects of their intestinal metabolites on human health have attracted the interest of researchers. This study evaluated the effects of three main colonic metabolites 3-(3,4-dihydroxyphenyl) propionic acid (3,4diOHPPA), 3-(3-hydroxyphenyl) propionic acid (3OHPPA) and 3-phenylpropionic acid (3PPA) of FA on longevity and stress resistance in C.elegans.

Results: Our results showed that 3,4diOHPPA, 3OHPPA and 3PPA extended the lifespan in normal conditions of C.elegans while FA did not. The high-doses of 3,4diOHPPA (0.5 mM), 3OHPPA (2.5 mM) and 3PPA (2.5 mM) prolonged the mean lifespan by 11.2%, 13.0% and 10.6%, respectively. Moreover, 3,4diOHPPA, 3OHPPA and 3PPA treatments promoted stress tolerance against heat, UV irradiation and paraquat. Furthermore, three metabolites ameliorated the physical functions including ROS and MDA levels, motility and pharyngeal pumping rate. The anti-aging activities mediated by 3,4diOHPPA, 3OHPPA and 3PPA depend on the HSF-1 and JNK-1 linked insulin/IGF-1 signaling pathway, which converge onto DAF-16.

Conclusion: The current findings suggest the colonic metabolites of FA have the potential to be used as anti-aging bioactivate compounds. This article is protected by copyright. All rights reserved.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/jsfa.12063DOI Listing
June 2022

Interaction between m6A methylation and noncoding RNA in glioma.

Cell Death Discov 2022 Jun 10;8(1):283. Epub 2022 Jun 10.

Department of Orthopedic Surgery, the First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, 310003, Zhejiang, China.

Glioma is considered to be the most common brain malignancy in the central nervous system. At present, the aetiology of glioma is not clear. Due to its rapidly growth and easily recurrence, the prognosis of patients with glioma is very poor. N6-methyladenosine (m6A) methylation is an internal reversible modification in most RNAs, including messenger RNAs (mRNAs) and noncoding RNAs (ncRNAs). Recent studies have shown that the m6A regulators are abnormal expressed, and are extensively involved in the progression of glioma by targeting ncRNAs. Moreover, as the most important epigenetic regulators, ncRNAs can also affect the function of m6A regulators in glioma. This review summarized the expression and function of certain common m6A regulators in glioma. Also, the current review sum up the mutual interactions between m6A regulators and ncRNAs in glioma.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41420-022-01075-5DOI Listing
June 2022

Is There a Sex Gap in Outcomes of Comparable Patients Supported with Left Ventricular Assist Devices?

Artif Organs 2022 Jun 10. Epub 2022 Jun 10.

Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany.

Objectives: Historically, females were described as suffering from worse outcomes after left ventricular assist device (LVAD) implantation. However, females' preoperative conditions are unique, making direct comparisons with males challenging. This study aimed to select through propensity score (PS) matching two preoperatively comparable populations of females and males and test if any real sex-related difference exists regarding survival and adverse events after LVAD implantation.

Methods: This retrospective single-center observational study investigated patients who received LVAD implantation between 2010 and 2018. PS matching was applied to balance preoperative heterogeneity between males and females. Primary endpoint was survival at follow-up. Secondary endpoints included perioperative outcomes and LVAD-related adverse events.

Results: 92 fully comparable females(n=46) and males(n=46) were selected after PS matching (median age:57 years, min-max:18-75). 26.1% of patients required preoperative mechanical circulatory support. Females needed more intraoperative fresh frozen plasma (p<0.001) and platelets transfusions (p=0.008) compared to males, but postoperative outcomes were comparable between groups. In-hospital, 1-year and 2-year survival were 78.3%, 69.6% and 65.2%, respectively, with no differences between groups. Survival probability remained comparable up to 8 years of follow-up(p=0.35). Overall, females showed a higher rate of strokes(p=0.039) compared to males in the follow-up time.

Conclusions: After reducing preoperative heterogeneity between females and males, survival after LVAD implantation does not differ based on sex. However, differences might exist in terms of higher transfusions and strokes in females. Reducing preoperative sex disparities and developing intraoperative and anticoagulation strategies which acknowledge sex-related variations might help abolishing differences in LVAD outcomes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/aor.14333DOI Listing
June 2022

A new LDH based sustained-release carbon source filter media to achieve advanced denitrogenation of low C/N wastewater at low temperature.

Sci Total Environ 2022 Sep 6;838(Pt 3):156488. Epub 2022 Jun 6.

State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, Jiangsu, PR China. Electronic address:

Advanced denitrogenation of wastewater is now facing major challenges brought by low C/N ratio and low temperature. The development of sustained-release materials with good and stable carbon release properties was an effective countermeasure. FeNi-Layered double-metal hydroxides (LDH)- sodium carboxymethyl cellulose (CMC) filter media and its potential use in heterotrophic and sulfur-based mixotrophic denitrification biological filter (DNBF), was firstly reported. It demonstrated stable structure and good carbon release performance with a mass transfer coefficient (K) of 4.40 mg·L·s. When the influent NO-N of 50 mg/L with the C/N ratio of 3 at 10 °C, the maximum nitrogen loading rate of 0.22 kg·N/(m·d) and effluent TN close to 5 mg/L (nitrogen removal of almost 90 %) could be achieved. The slowly released carbon source and the leached iron increased the abundance of denitrifying bacteria and functional genes, and the augmentation of Sulfuritalea and the secretion of biofilm protein stimulated by sulfur also played a synergistic role. This study provided a new potentially effective strategy to enhance advanced denitrification of wastewater of low C/N wastewater at low temperature.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2022.156488DOI Listing
September 2022

An aptamer-assisted biological nanopore biosensor for ultra-sensitive detection of ochratoxin A with a portable single-molecule measuring instrument.

Talanta 2022 Oct 27;248:123619. Epub 2022 May 27.

School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi'an, 710021, China. Electronic address:

Biological nanopore-based single-molecule detection technology has shown ultrahigh sensitivity to various target analyte. But the detection scope of interesting targets is limited due to the lack of effective signal conversion strategies. In addition, conventional nanopore detection instruments are cumbersome, resulting nanopore detection can only be performed in laboratory. Herein, a customizable nanopore current amplifier is constructed to lower the cost and increase the portability of the nanopore instrument, and then an immobilized aptamer-based signal conversion strategy is proposed for α-hemolysin (α-HL) nanopore to detect small molecules (ochratoxin A, OTA). The presence of OTA in sample would trigger the release of probe single-strand DNA (ssDNA) from magnetic beads, which could subsequently cause current blockage in nanopore. The results show that the signal frequency of probe ssDNA has a linear relationship with the OTA concentration in the range of 2 × 10~2 × 10 pmol/L. Compared to other methods, our sensing system has achieved an ultra-sensitive detection of OTA with the detection limit as low as 1.697 pmol/L. This strategy could broaden the scope of nanopore detection and have the potential for rapid and in-situ detection of other food contaminants in the future.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.talanta.2022.123619DOI Listing
October 2022

Bioinspired Amphibious Origami Robot with Body Sensing for Multimodal Locomotion.

Soft Robot 2022 Jun 7. Epub 2022 Jun 7.

Department of Biomedical Engineering, National University of Singapore, Singapore.

Animals have long captured the inspirations of researchers in robotics with their unrivaled capabilities of multimodal locomotion on land and in water, achieved by functionally versatile limbs. Conventional soft robots show infinite degrees-of-freedom (DOFs), making it hard to be actuated and conduct multiple movements especially for multimodal locomotion in different environments. An origami robot, which is capable of reversibly transforming the robotic shape by simple creases folding/unfolding, reveals advantages for imitating flexible movements of animals, thus drawing more and more attention. However, it poses substantial technological challenges for bioinspired design, sensing, and actuation of origami robots that can generate multimodal locomotion through performing complex morphologic deformation in different scenarios such as land and water. To relieve this issue, we propose a novel bioinspired amphibious origami machine with body sensing for multimodal locomotion. In this work, inspired by the peristalsis of inchworm and human swimming behaviors, a unique origami body with legs and origami arms is developed to enable the integrated robot to move both on land and in water. Instead of traditional electronic sensors, we design highly stretchable and foldable layer resistive sensor with conductive polymers coated onto the origami body to achieve robotic sensing such as obstacle detection. In addition, with detailed analysis, a self-designed pneumatic system of time division, multiplexing, and serialization is adopted to efficiently control the robot with high DOF. We eventually demonstrate that the fabricated origami robot successfully moves in amphibious environments, which is capable of crawling forward, turning right/left, and swimming. We expect that this work indicates contributions to advanced origami design, actuation control, and body sensor of the bioinspired robot with multimodal locomotion for broadly practical applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1089/soro.2021.0118DOI Listing
June 2022

Analysis of Human Information Recognition Model in Sports Based on Radial Basis Fuzzy Neural Network.

Comput Intell Neurosci 2022 26;2022:5625006. Epub 2022 May 26.

Yong In University, Yongin 17092, Republic of Korea.

In sports, because the movement of the human body is composed of the movements of the human limbs, and the complex and changeable movements of the human limbs lead to various and complicated movement modes of the entire human body, it is not easy to accurately track the human body movement. The recognition of human characteristic behavior belongs to a higher level computer vision topic, which is used to understand and describe the characteristic behavior of people, and there are also many research difficulties. Because the radial basis fuzzy neural network has the characteristics of parallel processing, nonlinearity, fault tolerance, self-adaptation, and self-learning, it has the advantage of high recognition efficiency when it is applied to the recognition of intersecting features and incomplete features. Therefore, this paper applies it to the analysis of the human body information recognition model in sports. The research results show that the human body information recognition model proposed in this paper has a high recognition accuracy and can detect the movement state of people in sports in real time and accurately.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2022/5625006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9162820PMC
June 2022

Hybrid imidazo[1,2-a]pyridine analogs as potent ATX inhibitors with concrete in vivo antifibrosis effect.

Arch Pharm (Weinheim) 2022 Jun 3:e2200171. Epub 2022 Jun 3.

Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang, People's Republic of China.

In recent years, small-molecule inhibitors targeting the autotaxin (ATX)/lysophosphatidic acid axis gradually brought excellent disease management benefits. Herein, a series of imidazo[1,2-a]pyridine compounds (1-11) were designed as ATX inhibitors through a hybrid strategy by combining the imidazo[1,2-a]pyridine skeleton in GLPG1690 and the benzyl carbamate moiety in PF-8380. As indicated by FS-3-based enzymatic assay, the carbamate derivatives revealed moderate to satisfying ATX inhibitory potency (IC  = 23-343 nM). Subsequently, the carbamate linker was altered to a urea moiety (12-19) with the aim of retaining ATX inhibition and improving the druglikeness profile. The binding mode analysis all over the modification process well rationalized the leading activity of urea derivatives in an enzymatic assay. Following further structural optimization, the diethanolamine derivative 19 exerted an amazing inhibitory activity (IC  = 3.98 nM) similar to the positive control GLPG1690 (IC  = 3.72 nM) and PF-8380 (IC  = 4.23 nM). Accordingly, 19 was tested directly for in vivo antifibrotic effects through a bleomycin model (H&E staining), in which 19 effectively alleviated lung structural damage and fibrosis at an oral dose of 20 and 60 mg/kg. Collectively, 19 qualified as a promising ATX inhibitor for potential application in fibrosis-relevant disease treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/ardp.202200171DOI Listing
June 2022

Endovascular Treatment Versus Best Medical Management in Acute Basilar Artery Occlusion Strokes: Results From the ATTENTION Multicenter Registry.

Circulation 2022 Jun 3:101161CIRCULATIONAHA121058544. Epub 2022 Jun 3.

Department of Neurology, Anqing Hospital Affiliated to Anhui Medical University, China (X.S.).

Background: The authors compare the effectiveness and safety of endovascular treatment (EVT) versus best medical management (BMM) in strokes attributable to acute basilar artery occlusion (BAO).

Methods: The present analysis was based on the ongoing, prospective, multicenter ATTENTION (Endovascular Treatment for Acute Basilar Artery Occlusion) trial registry in China. Our analytic sample comprised 2134 patients recruited at 48 sites between 2017 and 2021 and included 462 patients who received BMM and 1672 patients who received EVT. We performed an inversed probability of treatment weighting analysis. Qualifying patients had to present within 24 hours of estimated BAO. The primary clinical outcome was favorable functional outcome (modified Rankin Scale score, 0-3) at 90 days. We also performed a sensitivity analysis with the propensity score matching-based and the instrumental variable-based analysis.

Results: In our primary analysis using the inversed probability of treatment weighting-based analysis, there was a significantly higher rate of favorable outcome at 90 days among EVT patients compared with BMM-treated patients (adjusted relative risk, 1.42 [95% CI, 1.19-1.65]; absolute risk difference, 11.8% [95% CI, 6.9-16.7%]). The mortality was significantly lower (adjusted relative risk, 0.78 [95% CI, 0.69-0.88]; absolute risk difference, -10.3% [95% CI, -15.8 to -4.9]) in patients undergoing EVT. Results were generally consistent across the secondary end points. Similar associations were seen in the propensity score matching-based and instrumental variable-based analysis.

Conclusion: In this real-world study, EVT was associated with significantly better functional outcomes and survival at 90 days. Well-designed randomized studies comparing EVT with BMM in the acute BAO are needed.

Registration: URL: www.chictr.org.cn Unique identifier: ChiCTR2000041117.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1161/CIRCULATIONAHA.121.058544DOI Listing
June 2022

Effects of Exercise on Extracellular Vesicles in Patients with Metabolic Dysfunction: a Systematic Review.

J Cardiovasc Transl Res 2022 Jun 2. Epub 2022 Jun 2.

Key Laboratory of Chinese Internal Medicine of Ministry of Education, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, 100700, People's Republic of China.

The aim of this study was to investigate the effect of exercise on extracellular vesicles (EVs) in patients with metabolic dysfunction. The literatures were searched until Apr 28, 2022, and 16 studies that met inclusion criteria were included in this review. The results showed that the concentrations of platelet-derived extracellular vesicles (PEVs) and endothelial cell-derived extracellular vesicles (EEVs) decreased after long-term exercise, especially for CD62E EEVs and CD105 EEVs. Simultaneously, exercise improved the concentration of clinical evaluation indicators of metabolic diseases, and the changes in these indicators were positively correlated with the changes of EEVs and PEVs. The concentration of skeletal muscle-derived extracellular vesicles (SkEVs) increased after a single bout of exercise. The aforementioned results indicated that long-term exercise might improve endothelial function and hypercoagulability in patients with metabolic dysfunction. The changes in concentrations of EVs could assist in assessing effect of exercise on patients with metabolic dysfunction.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12265-022-10282-5DOI Listing
June 2022

Predictive Nomogram for Unfavorable Outcome of Spontaneous Intracerebral Hemorrhage.

World Neurosurg 2022 May 30. Epub 2022 May 30.

Department of Neurosurgery, Qingdao Municipal Hospital, Qingdao, Shandong, P. R. China. Electronic address:

Background: The goal of this retrospective study was to evaluate the effect of surgical timing on patient outcomes after spontaneous intracerebral hemorrhage (ICH). We also identified risk factors associated with poor prognosis.

Methods: We reviewed all patients who underwent surgery for ICH between January 2014 and January 2021. The outcome was measured using the modified Rankin Scale (mRS) score at 6 months after the surgery. Patients with mRS 0-2 were considered having favorable outcomes, and those with mRS 3-5 were considered having unfavorable outcomes. The relationships of surgical timing with the risk of unfavorable outcomes were identified using the interaction and stratified analyses, and generalized additive and logistic regression models. A nomogram was established and evaluated using a receiver operating characteristic curve analysis, plotted decision curve, and calibration curve.

Results: We identified 53 patients with favorable outcomes and 144 with unfavorable outcomes. The number of cases who underwent surgery at >12 hours and <36 hours in the favorable outcome group was more than that in the unfavorable outcome group (P < 0.001). When the time to operating room (TOR) was less than 21 hours, a shorter TOR was associated with unfavorable outcomes, using the smoothing spline analysis (odds ratio = 0.8, P < 0.001). Finally, we developed a nomogram using systolic blood pressure, Glasgow Coma Scale, midline shift, hematoma volume, and TOR for predicting the unfavorable outcome. The area under the curve, accuracy, specificity, and sensitivity of nomogram were 0.90, 0.87, 0.72, and 0.93, respectively.

Conclusion: Surgical timing between 12 and 26 hours after ICH was associated with favorable outcomes. The nomogram including systolic blood pressure, Glasgow Coma Scale, midline shift, hematoma volume, and TOR was reliable for predicting the ICH outcome.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.wneu.2022.05.111DOI Listing
May 2022

Association between fluid intake and extubation failure in intensive care unit patients with negative fluid balance: a retrospective observational study.

BMC Anesthesiol 2022 Jun 1;22(1):170. Epub 2022 Jun 1.

Department of Critical Care Medicine, Beijing Tongren Hospital, Capital Medical University, Beijing, China.

Background: Negative fluid balance (NFB) is associated with reduced extubation failure. However, whether achieving more NFB can further improve extubation outcome has not been investigated. This study aimed to investigate whether more NFB and restricted fluid intake were associated with extubation success.

Methods: We performed a retrospective study of adult patients with mechanical ventilation (MV) admitted to Medical Information Mart for Intensive Care (MIMIC-III) from 2001 to 2012. Patients with duration of MV over 24 hours and NFB within 24 hours before extubation were included for analysis. The primary outcome was extubation failure, defined as reintubation within 72 hours after extubation. Association between fluid balance or fluid intake and extubation outcome were investigated with multivariable logistic models.

Results: A total of 3433 extubation events were recorded. 1803 with NFB were included for the final analysis, of which 201(11.1%) were extubation failure. Compared with slight NFB (- 20 to 0 ml/kg), more NFB were not associated improved extubation outcome. Compared with moderate fluid intake (30 to 60 ml/kg), lower (< 30 ml/kg, OR 0.75, 95% CI [0.54, 1.05], p = 0.088) or higher (> 60 ml/kg, OR 1.63, 95% CI [0.73, 3.35], p = 0.206) fluid intake was not associated with extubation outcome. Duration of MV, chronic obstructive pulmonary disease (COPD), hypercapnia, use of diuretics, and SAPSIIscore were associated with extubation failure.

Conclusions: More NFB or restricted fluid intake were not associated with reduced extubation failure in patients with NFB. However, for COPD patients, restricted fluid intake was associated with extubation success.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12871-022-01708-3DOI Listing
June 2022

Targeting C3b/C4b and VEGF with a bispecific fusion protein optimized for neovascular age-related macular degeneration therapy.

Sci Transl Med 2022 Jun 1;14(647):eabj2177. Epub 2022 Jun 1.

Innovent Biologics Inc., Suzhou 215000, China.

Antiangiogenesis therapies targeting vascular endothelial growth factor (VEGF) have revolutionized the treatment of neovascular ocular diseases, including neovascular age-related macular degeneration (nAMD). Compelling evidence has implicated the vital role of complement system dysregulation in AMD pathogenesis, implying it as a potential therapeutic strategy for geographic atrophy in dry AMD and to enhance the efficacy of anti-VEGF monotherapies in nAMD. This study reports the preclinical assessment and phase 1 clinical outcomes of a bispecific fusion protein, efdamrofusp alfa (code: IBI302), which is capable of neutralizing both VEGF isoforms and C3b/C4b. Efdamrofusp alfa showed superior efficacy over anti-VEGF monotherapy in a mouse laser-induced choroidal neovascularization (CNV) model after intravitreal delivery. Dual inhibition of VEGF and the complement activation was found to further inhibit macrophage infiltration and M2 macrophage polarization. Intravitreal efdamrofusp alfa demonstrated favorable safety profiles and exhibited antiangiogenetic efficacy in a nonhuman primate laser-induced CNV model. A phase 1 dose-escalating clinical trial (NCT03814291) was thus conducted on the basis of the preclinical data. Preliminary results showed that efdamrofusp alfa was well tolerated in patients with nAMD. These data suggest that efdamrofusp alfa might be effective for treating nAMD and possibly other complement-related ocular conditions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1126/scitranslmed.abj2177DOI Listing
June 2022

Exercise Intervention as a Therapy in Patients with Diabetes Mellitus and Sarcopenia: A Meta-Analysis.

Diabetes Ther 2022 Jun 1. Epub 2022 Jun 1.

Department of Internal Medicine, Beijing Chaoyang Hospital, Chaoyang, Beijing, 100020, People's Republic of China.

Introduction: Sarcopenia is defined as a progressive and generalized muscle disorder that involves accelerated loss of muscle mass and impaired function. It is believed to influence the ability to carry out daily activities, muscle strength, and physical capacity in the elderly. Studies have shown that sarcopenia has been implicated as both a cause and a consequence of diabetes mellitus. In this analysis, we aimed to systematically show the impact of exercise intervention as a therapy for patients with diabetes mellitus and sarcopenia.

Methods: Electronic databases, including PubMed, EMBASE, Web of Science, and the Cochrane database, were searched from November to December 2021 for publications based on exercise intervention in patients with sarcopenia. After the selection of studies for this analysis, patients with diabetes mellitus were retrieved. Since dichotomous data including mean and standard deviation were reported, weighted mean difference (MD) with 95% confidence intervals (CI) were used to represent the data following analysis.

Results: A total of 431 participants with diabetes mellitus and sarcopenia were included in this meta-analysis. A statistical analysis was carried out on patients with diabetes mellitus who were assigned to the exercise intervention group. Our analysis showed that "sit-to-stand test" and "timed up and go" were significantly in favor of exercise intervention: MD -1.57, 95% confidence interval (CI) -2.26 to -0.87 (P = 0.0001) versus MD -0.61, 95% CI -1.21 to -0.01 (P = 0.05), respectively. Handgrip strength, walking speed and leg strength were also assessed. Another statistical analysis was carried out, this time on patients with diabetes mellitus and sarcopenia who were not assigned to an exercise intervention. The results showed no significant difference among sit-to-stand test, timed up and go, handgrip strength, and leg strength.

Conclusion: Exercise intervention significantly improved the time taken to stand up from a sitting position, and to "stand up and go" in patients with diabetes mellitus and sarcopenia. Therefore, exercise intervention should be considered a relevant therapy for such patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s13300-022-01275-3DOI Listing
June 2022

Promoting Survivor Safety in Immigrant Communities: Online Simulation Training for Korean American Faith Leaders.

J Interpers Violence 2022 May 31:8862605221101189. Epub 2022 May 31.

College of Education, 311285Penn State University, University Park, PA, USA.

This study examined the efficacy of a short virtual case simulation for Korean American (KA) faith leaders, "Religious Leaders for Healthy Families." The goal of the program is to increase knowledge about intimate partner violence (IPV) and healthy intimate partner relationships, enhance self-efficacy in IPV prevention and intervention, strengthen attitudes that support their roles on IPV prevention and intervention, increase positive outcome expectations of their actions, and increase behavioral intentions and behaviors on IPV prevention and intervention. KA faith leaders from two large metropolitan areas with a high concentration of KA immigrants were invited to participate in the study (=102). Participants completed three online assessments: baseline, a 3-month, and a 6-month follow-up. After the baseline assessment, participants were randomized to either intervention ( = 53) or control ( = 49). The intervention consisted of four online simulation modules, each taking approximately 15-20 min to complete. At the 6-month follow-up, faith leaders in the intervention group significantly increased their knowledge and self-efficacy in IPV prevention and intervention compared to the control group. Mean scores for attitudes against IPV and prevention behaviors increased from baseline to the 6-month follow-up for the intervention group more than the control group, but the differences were not statistically significant. "Religious Leaders for Healthy Families" has the potential to reduce disparities in accessing resources and services for immigrant survivors of IPV. With its ease of use, this short, free online intervention has a high potential for uptake among faith leaders. Results are promising, but the COVID-19 pandemic negatively affected the study, with participants having scarce opportunities to practice the skills learned from the intervention. A larger follow-up study that combines "Religious Leaders for Healthy Families" with a community-wide intervention that targets all community members is warranted to reach more faith leaders and community members.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/08862605221101189DOI Listing
May 2022

High-Frequency Notable HBV Mutations Identified in Blood Donors With Occult Hepatitis B Infection From Heyuan City of Southern China.

Front Immunol 2022 13;13:754383. Epub 2022 May 13.

The Joint Laboratory on Transfusion-Transmitted Diseases (TTDs) between Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Nanning Blood Center, Nanning Blood Center, Nanning, China.

Background: All Chinese blood centers have implemented mini pool (MP) HBV nucleic acid testing (NAT) together with HBsAg ELISA in routine donor screening since 2015. The prevalence of occult hepatitis B virus infection (OBI) in donors from different regions varies, and the molecular characterization of the HBV DNA and clinical outcomes of these OBIs remain largely unexplored.

Methods: Blood donations from Heyuan city in Southern China were screened by HBsAg ELISA and HBV MP8 NAT. Donations with HBsAg-/HBV DNA+ were collected for this study. Molecular characterizations of HBV DNAs were further analyzed by various DNA amplification assays including quantitative PCR (qPCR) and nested PCR, amplifying the basic core and pre-core promoter regions (BCP/PC). The HBsAg (S) region from HBV DNA was isolated by high-volume nucleic acid extraction. Notable mutations were identified by comparison to the HBV reference sequences. The clinical outcomes of the donors with OBIs were further followed for nearly 3 years.

Results: Seventy OBIs from 44,592 donations (0.15%) that we identified and reported previously were enrolled for this current study. HBV sequences were obtained from 44/70 OBIs, and genotyping analysis showed that 42/44 (95.2%) OBIs were genotype B, and 2/44 (4.8%) were genotype C. Interestingly, mutation analysis revealed that various mutations including M133L/T, F134L, P142L, V168A, R169H, S174N, L175S, and V177A of HBV DNA affecting HBsAg detection were observed in genotype B OBIs. Two notable mutations, T47K and L53S, were identified in genotype C OBIs. Follow-up studies showed that 3/31 (9.7%) OBIs converted to HBsAg+ as chronic infections while 1/31 (3.2%) HBV DNA was undetectable (classified as recovery) and 27/31 (87.1%) remained as OBIs.

Conclusion: Various notable mutations affecting HBsAg detection were observed in blood donors with OBIs in Heyuan city of Southern China. Follow-up studies showed that most OBIs remained as OBIs with fluctuating or low viral loads. Higher sensitive HBV ID NAT is recommended for donor screening to further reduce the transmission risk of OBIs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2022.754383DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9136029PMC
May 2022

Bioinspired and Biomimetic Nanomedicines for Targeted Cancer Therapy.

Pharmaceutics 2022 May 23;14(5). Epub 2022 May 23.

Laboratory of Human Diseases and Immunotherapies, West China Hospital, Sichuan University, Chengdu 610041, China.

Undesirable side effects and multidrug resistance are the major obstacles in conventional chemotherapy towards cancers. Nanomedicines provide alternative strategies for tumor-targeted therapy due to their inherent properties, such as nanoscale size and tunable surface features. However, the applications of nanomedicines are hampered in vivo due to intrinsic disadvantages, such as poor abilities to cross biological barriers and unexpected off-target effects. Fortunately, biomimetic nanomedicines are emerging as promising therapeutics to maximize anti-tumor efficacy with minimal adverse effects due to their good biocompatibility and high accumulation abilities. These bioengineered agents incorporate both the physicochemical properties of diverse functional materials and the advantages of biological materials to achieve desired purposes, such as prolonged circulation time, specific targeting of tumor cells, and immune modulation. Among biological materials, mammalian cells (such as red blood cells, macrophages, monocytes, and neutrophils) and pathogens (such as viruses, bacteria, and fungi) are the functional components most often used to confer synthetic nanoparticles with the complex functionalities necessary for effective nano-biointeractions. In this review, we focus on recent advances in the development of bioinspired and biomimetic nanomedicines (such as mammalian cell-based drug delivery systems and pathogen-based nanoparticles) for targeted cancer therapy. We also discuss the biological influences and limitations of synthetic materials on the therapeutic effects and targeted efficacies of various nanomedicines.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/pharmaceutics14051109DOI Listing
May 2022

Core-Shell Covalently Linked Graphitic Carbon Nitride-Melamine-Resorcinol-Formaldehyde Microsphere Polymers for Efficient Photocatalytic CO Reduction to Methanol.

J Am Chem Soc 2022 Jun 27;144(22):9576-9585. Epub 2022 May 27.

Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois 60208, United States.

Photocatalytic reduction of CO with light and HO to form CHOH is a promising route to mitigate carbon emissions and climate changes. Although semiconducting metal oxides are potential photocatalysts for this reaction, low photon efficiency and leaching of environmentally unfriendly toxic metals limit their applicability. Here, we report metal-free, core-shell photocatalysts consisting of graphitic carbon nitride (g-CN, CN) covalently linked to melamine-resorcinol-formaldehyde (MRF) microsphere polymers for this reaction. Covalent linkage enabled efficient separation of photo-generated carriers and photocatalysis. Using 100 mg of a photocatalyst containing 15 wt % CN, a CHOH yield of 0.99 μmol·h was achieved at a reaction temperature of 80 °C and 0.5 MPa with external quantum efficiencies ranging from 5.5% at 380 nm to 1.7% at 550 nm. The yield was about 20 and 10 times higher than that of its components CN and MRF, respectively. Characterization with X-ray photoelectron spectroscopy, transmission electron microscopy, and bulk and surface elemental analyses supported the formation of a core-shell structure and the charge transfer in the C-N bond at the CN-MRF interface between the methoxy group in the 2,4-dihydroxylmethyl-1,3-diphenol part of MRF and the terminal amino groups in CN. This enhanced ligand-to-ligand charge transfer resulted in 67% of the photo-excited internal charge transferred from CN to the hydroxymethylamino group in MRF, whose amino group was the catalytic site for the CO photocatalytic reduction to CHOH. This study provides a series of new metal-free photocatalyst designs and insights into the molecular-level structure-mediated photocatalytic response.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/jacs.1c13301DOI Listing
June 2022

Effect of Extracellular Vesicles From Multiple Cells on Vascular Smooth Muscle Cells in Atherosclerosis.

Front Pharmacol 2022 10;13:857331. Epub 2022 May 10.

Key Laboratory of Chinese Internal Medicine of Ministry of Education, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China.

Atherosclerosis (AS)-related diseases are still the main cause of death in clinical patients. The phenotype switching, proliferation, migration, and secretion of vascular smooth muscle cells (VSMCs) have a pivotal role in atherosclerosis. Although numerous research studies have elucidated the role of VSMCs in AS, their potential functional regulations continue to be explored. The formation of AS involves various cells, such as endothelial cells, smooth muscle cells, and macrophages. Therefore, intercellular communication of blood vessels cannot be ignored due to closely connected endothelia, media, and adventitia. Extracellular vesicles (EVs), as the vectors of cell-to-cell communication, can deliver proteins and nucleic acids of parent cells to the recipient cells. EVs have emerged as being central in intercellular communication and play a vital role in the pathophysiologic mechanisms of AS. This review summarizes the effects of extracellular vesicles (EVs) derived from multiple cells (endothelial cells, macrophages, mesenchymal stem cells, etc.) on VSMCs in AS. The key findings of this review are as follows: 1) endothelial cell-derived EVs (EEVs) have anti- or pro-atherogenic effects on VSMCs; 2) macrophage-derived EVs (MEVs) aggravate the proliferation and migration of VSMCs; 3) mesenchymal stem cells can inhibit VSMCs; and 4) the proliferation and migration of VSMCs can be inhibited by the treatment of EVs with atherosclerosis-protective factors and promoted by noxious stimulants. These results suggested that EVs have the same functional properties as treated parent cells, which might provide vital guidance for treating AS.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fphar.2022.857331DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9127356PMC
May 2022

One-step synthesis of blue emission copper nanoclusters for the detection of furaltadone and temperature.

Spectrochim Acta A Mol Biomol Spectrosc 2022 Oct 20;279:121408. Epub 2022 May 20.

Department of Chemistry, Taiyuan Normal University, Jinzhong 030619, China.

Polyvinyl pyrrolidone (PVP), playing roles as a templating agent, can be applied to prepare blue-emitting copper nanoclusters (Cu [email protected]) on the basis of a rapid chemical reduction synthesis method. The Cu [email protected] displayed a blue emission wavelength at 430 nm and the corresponding quantum yield (QY) could reach 10.4%. Subsequently, the as-synthesized Cu [email protected] were used for the trace analysis of furaltadone based on the inner filter effect (IFE) between Cu [email protected] and furaltadone, which caused the fluorescence to be effectively quenched. Additionally, this proposed determination platform based on the Cu [email protected] for furaltadone sensing possessed an excellent linear range from 0.5 to 100 μM with a lower detection limit of 0.045 μM (S/N = 3). Meanwhile, the Cu [email protected] also could be applied for the sensing of temperature. Furthermore, the practicability of the sensing platform has been successfully verified by measuring furaltadone in real samples, affirming its potential to increase fields for the determination of furaltadone.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.saa.2022.121408DOI Listing
October 2022

MUSE: Visual Analysis of Musical Semantic Sequence.

IEEE Trans Vis Comput Graph 2022 May 24;PP. Epub 2022 May 24.

Visualization has the capacity of converting auditory perceptions of music into visual perceptions, which consequently opens the door to music visualization (e.g., exploring group style transitions and analyzing performance details). Current research either focuses on low-level analysis without constructing and comparing music group characteristics, or concentrates on high-level group analysis without analyzing and exploring detailed information. To fill this gap, integrating the high-level group analysis and low-level details exploration of music, we design a musical semantic sequence visualization analytics prototype system (MUSE) that mainly combines a distribution view and a semantic detail view, assisting analysts in obtaining the group characteristics and detailed interpretation. In the MUSE, we decompose the music into note sequences for modeling and abstracting music into three progressively fine-grained pieces of information (i.e., genres, instruments and notes). The distribution view integrates a new density contour, which considers sequence distance and semantic similarity, and helps analysts quickly identify the distribution features of the music group. The semantic detail view displays the music note sequences and combines the window moving to avoid visual clutter while ensuring the presentation of complete semantic details. To prove the usefulness and effectiveness of MUSE, we perform two case studies based on real-world music MIDI data. In addition, we conduct a quantitative user study and an expert evaluation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1109/TVCG.2022.3175364DOI Listing
May 2022
-->