Publications by authors named "Tong Chen"

664 Publications

Distribution and molecular characterization of avian infectious bronchitis virus in southern China.

Poult Sci 2021 Mar 27;100(7):101169. Epub 2021 Mar 27.

Guangdong Provincial Animal Virus Vector Vaccine Engineering Technology Research Center, College of Animal Science, South China Agricultural University, Guangzhou, 510642, P.R. China; Key Laboratory of Healthy Animal Husbandry and Environmental Control of Guangdong Province, Guangzhou, 510642, Guangdong, P.R. China. Electronic address:

Avian infectious bronchitis virus (IBV) is causing considerable economic losses in the world poultry industry. The main difficulty of prevention and control of IB disease is the numerous genotypes and serotypes. The genetic analysis of IBV was mainly based on the S1 gene which played an important role in infectivity. In the study, One hundred and thirty-nine strains of avian infectious bronchitis virus were isolated from chickens showing signs of disease in southern China during the period from April 2019 to March 2020. The nucleotide and amino acid sequences from the isolated field strains were compared to 22 published references. Nucleotide homologies ranged from 64.5% to 100% and amino acid homologies ranging from 70% to 99.8%. Six genotype IBV strains were co-circulating in southern China. QX-type was still the most dominant genotype. Alignment of nucleotide and amino acid sequences of S1 gene revealed that the substitutions, insertions and deletions are widely among isolated strains. Recombination analysis showed that there is a large number of recombinant strains amongst these isolates, forming new sub branches, subtypes and variants. Therefore, long-term continuing surveillance is necessary for IBV prevention and control.
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http://dx.doi.org/10.1016/j.psj.2021.101169DOI Listing
March 2021

βKlotho, a direct target of miR-206, contributes to the growth of hepatoblastoma through augmenting PI3K/Akt/mTOR signaling.

Am J Cancer Res 2021 15;11(5):1982-2004. Epub 2021 May 15.

Department of General Surgery, Shanghai Children's Hospital, Shanghai Jiao Tong University Shanghai 200040, P. R. China.

Hepatoblastoma (HB) is the most frequent pediatric liver malignancy. However, the treatment outcome for patients with advanced-stage HB remains unsatisfactory. Accumulating evidence indicates that βKlotho (KLB) acts as an oncogene or a tumor-suppressor gene in a context-dependent manner. Despite this, the expression profile and effects of KLB on the growth of HB are still elusive. This study aimed to explore the effect of miR-206/KLB axis on HB growth. The expression of KLB was explored in HB cells (HepG2 and HuH6) and tissues using quantitative polymerase chain reaction (qPCR), Western blot analysis, and immunohistochemistry. Besides, miR-206 expression was determined in HB cells and tissues using qPCR and fluorescence in situ hybridization. The prognostic value of KLB or miR-206 in our patients with HB was investigated using the Kaplan-Meier method. The biological effects of KLB or miR-206 on HB cells were identified . The proliferative effects of KLB on HuH6 cells were also investigated . Moreover, the mechanical signaling of KLB in HB was determined through bioinformatics analysis followed by experimental validation. The results showed a significant upregulation of KLB in HB tissues and cells. Elevated level of KLB was found to be significantly correlated with the aggressive phenotype and poor overall survival for children with HB. The function assay demonstrated that KLB knockdown promoted apoptosis and suppressed the proliferation, migration, and invasion of HB cells. Besides, KLB knockdown inhibited the proliferation of HuH6 cells , while KLB overexpression had the opposite effect. Furthermore, KLB was proved to be the direct target of miR-206. Low level of miR-206 served as an independent risk factor for poor prognosis in children with HB. The overexpression of miR-206 negatively regulated the aggressive biological behaviors of HB cells, which was partially rescued by KLB overexpression. Mechanically, the miR-206/KLB axis played a vital role in HB growth through augmenting the phosphatidylinositol 3-kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) signaling. In conclusion, the data demonstrated that the miR-206/KLB axis might serve as an important biomarker/therapeutic target for HB.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8167675PMC
May 2021

Distinctive gene expression patterns and imprinting signatures revealed in reciprocal crosses between cattle sub-species.

BMC Genomics 2021 Jun 3;22(1):410. Epub 2021 Jun 3.

Davies Research Centre, School of Animal and Veterinary Sciences, The University of Adelaide, Adelaide, Australia.

Background: There are two genetically distinct subspecies of cattle, Bos taurus taurus and Bos taurus indicus, which arose from independent domestication events. The two types of cattle show substantial phenotypic differences, some of which emerge during fetal development and are reflected in birth outcomes, including birth weight. We explored gene expression profiles in the placenta and four fetal tissues at mid-gestation from one taurine (Bos taurus taurus; Angus) and one indicine (Bos taurus indicus; Brahman) breed and their reciprocal crosses.

Results: In total 120 samples were analysed from a pure taurine breed, an indicine breed and their reciprocal cross fetuses, which identified 6456 differentially expressed genes (DEGs) between the two pure breeds in at least one fetal tissue of which 110 genes were differentially expressed in all five tissues examined. DEGs shared across tissues were enriched for pathways related to immune and stress response functions. Only the liver had a substantial number of DEGs when reciprocal crossed were compared among which 310 DEGs were found to be in common with DEGs identified between purebred livers; these DEGs were significantly enriched for metabolic process GO terms. Analysis of DEGs across purebred and crossbred tissues suggested an additive expression pattern for most genes, where both paternal and maternal alleles contributed to variation in gene expression levels. However, expression of 5% of DEGs in each tissue was consistent with parent of origin effects, with both paternal and maternal dominance effects identified.

Conclusions: These data identify candidate genes potentially driving the tissue-specific differences between these taurine and indicine breeds and provide a biological insight into parental genome effects underlying phenotypic differences in bovine fetal development.
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http://dx.doi.org/10.1186/s12864-021-07667-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176687PMC
June 2021

Expression and Biochemical Characterization of a Novel Marine Chitosanase from Suitable for Preparation of Chitobiose.

Mar Drugs 2021 May 24;19(6). Epub 2021 May 24.

National Key Laboratory of Biochemical Engineering, National Engineering Research Center for Biotechnology (Beijing), Key Laboratory of Biopharmaceutical Production & Formulation Engineering, PLA, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.

It is known that bioactivities of chitooligosaccharide (COS) are closely related to the degree of polymerization (DP); therefore, it is essential to prepare COS with controllable DP, such as chitobiose showing high antioxidant and antihyperlipidemia activities. In this study, BLAST, sequence alignment and phylogenetic analysis of characterized glycoside hydrolase (GH) 46 -chitosanases revealed that a chitosanase Sn1-CSN from was different from others. Sn1-CSN was overexpressed in , purified and characterized in detail. It showed the highest activity at pH 6.0 and exhibited superior stability between pH 4.0 and pH 11.0. Sn1-CSN displayed the highest activity at 50 °C and was fairly stable at ≤45 °C. Its apparent kinetic parameters against chitosan (DDA: degree of deacetylation, >94%) were determined, with and values of 1.8 mg/mL and 88.3 s, respectively. Cu enhanced the activity of Sn1-CSN by 54.2%, whereas Fe inhibited activity by 15.1%. Hydrolysis products of chitosan (DDA > 94%) by Sn1-CSN were mainly composed of chitobiose (87.3%), whereas partially acetylated chitosan with DDA 69% was mainly converted into partially acetylated COS with DP 2-13. This -chitosanase has great potential to be used for the preparation of chitobiose and partially acetylated COS with different DPs.
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http://dx.doi.org/10.3390/md19060300DOI Listing
May 2021

Correlation between the Nonperfusion Area on Ultra-Widefield Fluorescein Angiography and Nonflow Area on Optical Coherence Tomographic Angiography in Retinal Vein Occlusion.

J Ophthalmol 2021 30;2021:5581319. Epub 2021 Apr 30.

Medical Doctor, Department of Ophthalmology, Beijing Hospital, National Center of Gerontology, No. 1 Dahua Road Dongcheng District, Beijing, China.

Aims: To compare the relationship between the nonperfusion area (NPA) on ultra-widefield fluorescein angiography (UWFFA) and the nonflow area (NFA) on optical coherence tomographic angiography (OCTA) in retinal vein occlusion (RVO).

Methods: Cross-sectional study. 46 eyes of 46 RVO patients who underwent UWFFA and OCTA. NPA and ischemic index (ISI) were quantified on UWWFA. NFA, vessel density (VD) of the superficial capillary plexus (SCP), the deep capillary plexus (DCP), and the size foveal avascular zone (FAZ) on 3 ∗ 3 mm OCTA were measured. The association of the NPA and ISI on UWWFA and the parameters on OCTA were analyzed. Spearman correlation was used for statistical testing.

Results: The NPA and ISI on UWFFA were significantly correlated with the NFA on OCTA in RVO, and values were 0.688 ( < 0.01) and 0.680 ( < 0.01), respectively. VD in the SCP of the temporal quadrant was negatively correlated with NPA and ISI, and values were -0.346 ( < 0.05) and -0.337 ( < 0.05), respectively. VD in the DCP of the temporal quadrant was negatively correlated with the NPA, and the value was -0.246 ( < 0.05). No significant correlation was found between the NPA and ISI on UWFFA and VD of other quadrants in the SCP or DCP and the FAZ area on OCTA.

Conclusion: NPA in the peripheral retina was correlated with NFA in macula. NFA detected by OCTA could be an indicator of the ischemic status in RVO.
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http://dx.doi.org/10.1155/2021/5581319DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8110371PMC
April 2021

Systematic selection of microsatellite for paternity testing of yak.

Anim Genet 2021 May 17. Epub 2021 May 17.

Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Exploitation of Ministry of Education, Southwest Minzu University, Chengdu, 610041, China.

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http://dx.doi.org/10.1111/age.13080DOI Listing
May 2021

: A Review of Ethnomedicinal, Phytochemical, Pharmacological, and Toxicological Findings.

Front Chem 2021 29;9:666280. Epub 2021 Apr 29.

College of Animal Sciences, Jilin University, Changchun, China.

is a dry mature seed of Vaccaria hispanica (Mill.) Rauschert, which belongs to the genus (Neck.) Garcke. There are multiple medicinal parts of , according to the records, including roots, stems, leaves, flowers, and seeds, which should be used together. Currently, is most frequently used in the treatment of menstruation, dysmenorrhea, breast milk stoppages, and chylorrhea. Numerous studies present historical evidence of the use of to treat several diseases and describe its beneficial effects including prolactin- (PRL-) like, estrogen-like, antitumor, antiangiogenesis, and antioxidant activity. We summarized the period from January 1980 to December 2019 regarding . This review paper indicates that has promising clinical applications. The main active ingredients of the plant have been elucidated in recent years. We summarized the previously and newly discovered pharmacological effects of in addition to its active ingredients, ethnopharmacological uses, and toxicological properties, and provided a focus for future research.
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http://dx.doi.org/10.3389/fchem.2021.666280DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8117358PMC
April 2021

Therapeutic Mechanism and Effect of Camptothecin on Dextran Sodium Sulfate-Induced Ulcerative Colitis in Mice.

J Immunol Res 2021 24;2021:5556659. Epub 2021 Apr 24.

Department of Gastrointestinal Colorectal and Anal Surgery, China-Japan Union Hospital of Jilin University, Changchun 130033, China.

Camptothecin (CPT) is a cytotoxic quinoline alkaloid isolated from the bark and branches of the Chinese tree . CPT inhibits topoisomerase I. It possesses various antitumor activities and is mainly used in the treatment of colon, ovarian, liver, and bone cancers as well as leukemia. CPT inhibits the expressions of inflammatory genes and can prevent death from chronic inflammation. Therefore, we investigated the effect of CPT treatment in ulcerative colitis (UC) using DSS-induced UC mouse model; after that, we explored its potential mechanisms. Here, we found that CPT exerted protection on DSS-induced UC in rats. In addition, the administration prominently reduced the disease activity index as well as colon length of the model rats and remarkably reduced the inflammatory cytokines. Further, CPT significantly reduced several vital proinflammatory proteins in LPS-induced RAW264.7 cells. In summary, our findings demonstrate that CPT is hopefully to act as a therapeutic agent for UC.
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http://dx.doi.org/10.1155/2021/5556659DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8093050PMC
April 2021

Comparative Utility of Genetic Determinants of Drug Resistance and Phenotypic Drug Susceptibility Profiling in Predicting Clinical Outcomes in Patients With Multidrug-Resistant .

Front Public Health 2021 22;9:663974. Epub 2021 Apr 22.

School of International Pharmaceutical Business, China Pharmaceutical University, Nanjing, China.

Programmatic management of drug-resistant tuberculosis in Ningbo, China. To assess whether data-driven genetic determinants of drug resistance patterns could outperform phenotypic drug susceptibility testing in predicting clinical meaningful outcomes among patients with multidrug-resistant tuberculosis (MDR-TB). We conducted a prospective cohort study of 104 MDR-TB patients. All MDR-TB isolates underwent drug susceptibility testing and genotyping for mutations that could cause drug resistance. Study outcomes were time to sputum smear conversion and probability of treatment success, as well as time to culture conversion within 6 months. Data were analyzed using latent class analysis, Kaplan-Meier curves, and Cox regression models. We report that latent class analysis of data identified two latent classes that predicted sputum smear conversion with = 0.001 and area under receiver-operating characteristic curve of 0.73. The predicted latent class memberships were associated with superior capability in predicting sputum culture conversion at 6 months and overall treatment success compared to phenotypic drug susceptibility profiling using boosted logistic regression models. These results suggest that genetic determinants of drug resistance in combination with phenotypic drug-resistant tests could serve as useful biomarkers in predicting treatment prognosis in MDR-TB.
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http://dx.doi.org/10.3389/fpubh.2021.663974DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100237PMC
May 2021

Current and emerging therapies for primary central nervous system lymphoma.

Biomark Res 2021 May 6;9(1):32. Epub 2021 May 6.

Department of Hematology, Huashan Hospital, Fudan University, 12 Wulumuqi Middle Road, Shanghai, 200040, China.

Primary central nervous system (CNS) lymphoma (PCNSL) is a rare type of extranodal lymphoma exclusively involving the CNS at the onset, with diffuse large B-cell lymphoma (DLBCL) as the most common histological subtype. As PCNSL is a malignancy arising in an immune-privileged site, suboptimal delivery of systemic agents into tumor tissues results in poorer outcomes in PCNSL than in non-CNS DLBCLs. Commonly used regimens for PCNSL include high-dose methotrexate-based chemotherapy with rituximab for induction therapy and intensive chemotherapy followed by autologous hematopoietic stem cell transplantation or whole-brain radiotherapy for consolidation therapy. Targeted agents against the B-cell receptor signaling pathway, microenvironment immunomodulation and blood-brain barrier (BBB) permeabilization appear to be promising in treating refractory/relapsed patients. Chimeric antigen receptor-T cells (CAR-T cells) have been shown to penetrate the BBB as a potential tool to manipulate this disease entity while controlling CAR-T cell-related encephalopathy syndrome. Future approaches may stratify patients according to age, performance status, molecular biomarkers and cellular bioinformation. This review summarizes the current therapies and emerging agents in clinical development for PCNSL treatment.
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http://dx.doi.org/10.1186/s40364-021-00282-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8101140PMC
May 2021

Association of Insulin Glargine Treatment with Bone Mineral Density in Patients with Type 2 Diabetes Mellitus.

Diabetes Metab Syndr Obes 2021 29;14:1909-1917. Epub 2021 Apr 29.

Department of Endocrinology, First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, People's Republic of China.

Purpose: To assess the association of type 2 diabetes mellitus (T2DM) and insulin glargine treatment with bone mineral density (BMD) in Chinese people.

Methods: This retrospective study included 50 subjects with T2DM: 25 received oral glucose-lowering medication (ORL group), and 25 received oral glucose-lowering medication in combination with insulin glargine injection (CGI group). Thirty non-diabetic control subjects were also included. BMD was measured at lumbar vertebrae 1-4 (L1-L4), spine bone mineral density (sBMD) results summary (L2-L4), femoral neck and trochanter by dual-energy x-ray absorptiometry.

Results: Compared with non-diabetic controls, people with T2DM had significantly lower mean BMD at L2 (1.073±0.120 vs 0.984±0.158), L3 (1.094±0.129 vs 0.991±0.163) and L4 (1.089±0.130 vs 0.982±0.165) (all P<0.05), significantly lower levels of serum calcium (2.02±0.22 vs 2.27±0.17 mmol/L, P<0.05), PTH (24.19±9.71 vs 31.52±8.96 pg/mL, P<0.05), and higher serum phosphate levels (1.43±0.37 vs 1.20±0.15 mmol/L, P<0.05). The CGI group had higher L2, L3 and L4 BMD and sBMD (L2-L4) (P<0.05), higher serum calcium levels (2.19±0.11 vs 1.98±0.20 mmol/L, P<0.05) and lower serum phosphate levels (1.28±0.20 vs 1.58±0.43 mmol/L, P<0.05) versus the ORL group. BMD and serum calcium levels were associated with the application of insulin glargine.

Conclusion: These results suggest that insulin glargine may affect bone metabolism in patients diagnosed with T2DM. The study has implications for the selection of hypoglycemic agents for diabetic patients at risk of osteoporosis.
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http://dx.doi.org/10.2147/DMSO.S302627DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8092849PMC
April 2021

Follicular fluid exosomes: Important modulator in proliferation and steroid synthesis of porcine granulosa cells.

FASEB J 2021 May;35(5):e21610

College of Animal Sciences, Jilin University, Changchun, China.

Granulosa cells (GCs) are regulated by various factors during ovarian development. However, there are few reports on the role of follicular fluid exosomes in ovarian GCs. In this study, porcine ovarian GCs were used to explore the effects of follicular fluid exosomes on GCs. GCs were treated with in vitro, and the changes in cell proliferation, steroid synthesis, and associated signal pathways were detected. The results showed that exosomes increased cell viability and altered the gene expression profile of GCs. Exosomes also increased the level of gene expression associated with both proliferation and progesterone synthesis, in which the MAPK/ERK and WNT/B-CATENIN pathways were involved. In addition, exosome-carried microRNAs were identified by high-throughput sequencing, and exosomal miR-31-5p was found to promote the proliferation of GCs and progesterone synthesis via the WNT/B-CATENIN pathway by targeting the SFRP4 follicle growth inhibitor. In conclusion, this study has demonstrated that exosomes are essential substances involved in regulating the physiological function of GCs.
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http://dx.doi.org/10.1096/fj.202100030RRDOI Listing
May 2021

Colorectal Cancer Stem Cell States Uncovered by Simultaneous Single-Cell Analysis of Transcriptome and Telomeres.

Adv Sci (Weinh) 2021 04 8;8(8):2004320. Epub 2021 Feb 8.

State Key Laboratory of Medicinal Chemical Biology Nankai University Tianjin 300350 China.

Cancer stem cells (CSCs) presumably contribute to tumor progression and drug resistance, yet their definitive features have remained elusive. Here, simultaneous measurement of telomere length and transcriptome in the same cells enables systematic assessment of CSCs in primary colorectal cancer (CRC). The in-depth transcriptome profiled by SMART-seq2 is independently validated by high-throughput scRNA-seq using 10 × Genomics. It is found that rare CSCs exist in dormant state and display plasticity toward cancer epithelial cells (EPCs) that essentially are presumptive tumor-initiating cells (TICs), while both retaining the prominent signaling pathways including WNT, TGF-, and HIPPO/YAP. Moreover, CSCs exhibit chromosome copy number variation (CNV) pattern resembling cancer EPCs but distinct from normal stem cells, suggesting the phylogenetic relationship between CSCs and cancer EPCs. Notably, CSCs maintain shorter telomeres and possess minimal telomerase activity consistent with their nonproliferative nature, unlike cancer EPCs. Additionally, the specific signature of CSCs particularly , , , , and correlates with the prognosis of CRC. These findings characterize the heterogeneity of CSCs and their linkage to cancer EPCs/TICs, some of which are conventionally regarded as CSCs.
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http://dx.doi.org/10.1002/advs.202004320DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8061397PMC
April 2021

Postoperative corneal biomechanics and influencing factors during femtosecond-assisted laser in situ keratomileusis (FS-LASIK) and laser-assisted subepithelial keratomileusis (LASEK) for high myopia.

Lasers Med Sci 2021 Apr 23. Epub 2021 Apr 23.

Eye Hospital of Shandong First Medical University, 372 Jingsi Road, Jinan, 250021, China.

The purpose of this study is to compare the postoperative corneal biomechanics and assess the influence factors after femtosecond-assisted laser in situ keratomileusis (FS-LASIK) and laser-assisted subepithelial keratomileusis (LASEK) for high myopia. In this retrospective study, patients who completed 1-year follow-up were included. The corneal biomechanical parameters, including deformation amplitude ratio 2.0 mm (DA ratio 2.0 mm), integrated inverse radius (IntInv Rad), stiffness parameter at first applanation (SP-A1), and Ambrosio relational thickness through the horizontal meridian (ARTh), were measured with Corvis STII. We also investigated the relationship between these biomechanics and preoperative or intraoperative variables. Thirty eyes had FS-LASIK and 30 eyes had LASEK. The changes in DA ratio 2.0 mm, IntInv Rad, and SP-A1 after surgery were significantly smaller in the LASEK group than in the FS-LASIK group, while the change in the ARTh was not significantly different between groups. No significant differences were detected in the mean values of postoperative DA ratio 2.0 mm, IntInv Rad, and SP-A1 between LASEK and FS-LASIK, while significant difference was detected in the mean value of postoperative ARTh. There was a significant correlation between the resident stromal bed thickness and the postoperative DA ratio 2.0 mm, IntInv Rad, or SP-A1. The postoperative ARTh has shown significant correlation with postoperative central corneal thickness and the amount of myopic correction. The effect of LASEK on corneal biomechanics was smaller than FS-LASIK when the same central corneal thickness was consumed. LASEK may be performed with a lower risk of postoperative corneal ectasia than FS-LASIK.
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http://dx.doi.org/10.1007/s10103-021-03320-2DOI Listing
April 2021

Solid-State Thermal Memory of Temperature-Responsive Polymer Induced by Hydrogen Bonds.

Nano Lett 2021 May 21;21(9):3843-3848. Epub 2021 Apr 21.

CAS Key Laboratory of Mechanical Behavior and Design of Materials, University of Science and Technology of China, Hefei 230026, P. R. China.

Memory is an essential element for a computer to process information, which is integrated by logical circuits. Like electronic computing, thermal information can also be stored and read out by a thermal memory. Here, we show that a phase-changing polymer with hysteretic thermal transport properties can be experimentally processed into thermal memories at room temperature. We used a temperature-responsive and reversible polymer synthesized with melamine (M) and 6,7-dimethoxy-2,4[1H,3H]-quinazolinedione (Q) as a model system to demonstrate the manipulation of thermal transport at a molecular level. Fourier transform infrared spectroscopy and differential scanning calorimetry measurements indicate that this hysteretic behavior is based on the interaction of hydrogen bonds at high (317 K) and low (297 K) temperatures. This work demonstrates a controllable phonon transport process through the manipulation of hydrogen bonds, and thus it has potential applications in thermal memories.
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http://dx.doi.org/10.1021/acs.nanolett.1c00289DOI Listing
May 2021

Transcription activation of microRNA-25 by PEA3 augments progression of gastric cancer through suppressing SIK1.

Exp Physiol 2021 Apr 20. Epub 2021 Apr 20.

Department of Gastrointestinal Colorectal and Anal Surgery, China-Japan Union Hospital of Jilin University, Changchun, Jilin, 130033, P.R. China.

New Findings: What is the central question of this study? What is the function of miR-25 in the development of gastric cancer (GC) and the molecular mechanism? What is the main finding and its importance? Polyomavirus enhancer activator protein 3 (PEA3) activates transcription of microRNA-25 and augments progression of GC through suppressing salt-inducible kinase 1 (SIK1) and activating the Wnt/β-catenin pathway.

Abstract: Gastric cancer (GC) is one of the leading causes of cancer-related death worldwide. Aberrant expression of microRNAs (miRNAs) is frequently involved in the pathogenesis of GC. This study aims to determine the functions of miR-25 in GC development and the molecular mechanism. miR-25 was screened to be significantly highly expressed in GC tissues using a miRNA microarray analysis. The subsequent RT-qPCR suggested high-expression profiling of miR-25 in cancer tissues and cells relative to the normal samples. High miR-25 expression indicated poor prognosis and shorter survival time of GC patients. Polyomavirus enhancer activator protein 3 (PEA3) was identified as an upstream transcriptional activator of miR-25. miR-25 directly bound to salt-inducible kinase 1 (SIK1) mRNA. Downregulation of miR-25 suppressed proliferation and invasiveness of GC cells and the growth of xenograft tumors in vivo. These tumor-suppressive effects were strengthened by further silencing of PEA3 but blocked by SIK1 knockdown. The PEA3/miR-25 axis was found to activate the Wnt/β-catenin signaling pathway. To conclude, this study suggested that PEA3 activates miR-25 transcription and regulates GC progression through suppressing SIK1 and activating the Wnt/β-catenin pathway. This study may provide new ideas in GC treatment. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1113/EP089254DOI Listing
April 2021

Does exercise training improve the function of vascular smooth muscle? A systematic review and meta-analysis.

Res Sports Med 2021 Apr 18:1-16. Epub 2021 Apr 18.

School of Sports and Health, Nanjing Sport Institute, Nanjing, Jiangsu, China.

We aimed to determine the effects of exercise training on the function of vascular smooth muscle cells. PubMed and Web of Science about the effects of exercise training on vascular smooth muscle cells were searched up to August 2020. The effect sizes were estimated in terms of the standardized mean difference. The number of studies included was thirty-five overall. Exercise training had positive effects on vascular smooth muscle cells function in participants older than 40. Effect sizes for HIGH intensity and MIX were positive but small, and also when training duration was longer than 12 weeks. We concluded that vascular smooth muscle cells response can be promoted by exercise training. Vigorous aerobic exercise and mixture training modality were the best ways to promote the dilation response of vascular smooth muscle cells. Additionally, the significant improvement induced by exercise training only occurred when training lasted for longer than 12 weeks.
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http://dx.doi.org/10.1080/15438627.2021.1917408DOI Listing
April 2021

LINC01977 Promotes Breast Cancer Progression and Chemoresistance to Doxorubicin by Targeting miR-212-3p/GOLM1 Axis.

Front Oncol 2021 31;11:657094. Epub 2021 Mar 31.

Department of Breast Surgery, General Surgery, Qilu Hospital of Shandong University, Jinan, China.

Long non-coding RNAs(lncRNAs) play an important role in cancer initiation and progression. However, hub lncRNAs involved in breast cancer still remain underexplored. In this study, integrated bioinformatics analysis was used to define LINC01977 as a key oncogenic driver in breast cancer. Subsequently, assays showed that LINC01977 could significantly promote breast cancer progression and chemoresistance to doxorubicin. To further investigate its biological mechanism, we performed dual-luciferase reporter assay, real-time PCR, RNA immunoprecipitation (RIP), and rescue assay. Our results indicated that LINC01977 may function as ceRNA to prevent GOLM1 gene from miRNA-mediated repression by sponging miR-212-3p. Overall, LINC01977 can serve as a novel prognostic indicator, and help develop more effective therapeutic approaches for breast cancer patients.
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http://dx.doi.org/10.3389/fonc.2021.657094DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8046671PMC
March 2021

Colostrum source and passive immunity transfer in dairy bull calves.

J Dairy Sci 2021 Apr 14. Epub 2021 Apr 14.

Davies Livestock Research Centre, School of Animal and Veterinary Sciences, University of Adelaide, Roseworthy Campus, Roseworthy, SA 5371, Australia.

Colostrum is essential for good neonate health; however, it is not known whether different calves absorb the nutrients from colostrum equally well. In this study, the absorption of protein, IgG, and γ-glutamyl transferase was compared in newborn dairy bull calves for 1 wk after feeding colostrum from different sources. Thirty-five Holstein-Friesian bull calves were randomly allocated into 3 groups and fed colostrum within 4 h after birth. Group A calves (n = 12) were bottle fed colostrum from their own dam for 3 d. Colostrum from these group A cows was also used as foster cow colostrum for the group B calves (n = 12), such that each group A and B calf pair received identical colostrum from each milking of the respective group A dam (10% of birth weight per day). The group C calves (n = 11) were fed 1 bottle (2 L) of pooled colostrum and transition milk (referred to as pooled colostrum), as was the standard practice on the dairy farm. The pooled colostrum was collected from the other dairy cows on the farm 0 to 4 d postpartum and stored at 4°C for less than 12 h. Blood was sampled from calves before the first feeding and at 1, 2, 3, and 7 d after birth. Levels of total solids, total protein, and IgG were higher in the dam colostrum than in the pooled colostrum. At birth, there were no differences between the calf groups for any measurements, and all calves had very low IgG levels. After receiving colostrum, the glucose, plasma γ-glutamyl transferase, serum total protein, and IgG concentrations increased significantly in all calves. There were no differences in any blood measurements at any time point between the pairs of group A and group B calves that received colostrum from the same cow except for the IgG concentration 2 d after birth. However, the group A calves had a higher total serum protein level and IgG concentration than the group C calves for all the time points after the first feeding. The group B calves had a higher IgG concentration than the group C calves on d 1, 2, and 7 after birth. Compared with groups A and B, there was no difference in the proportion of calves in group C that failed to have passive immunity transferred adequately based on the IgG threshold (<10 g/L). Thus, the calves receiving identical colostrum from the same cow had the same levels of IgG, and even the pooled colostrum provided sufficient transfer of IgG as the calves were fed within 4 h after birth.
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http://dx.doi.org/10.3168/jds.2020-19318DOI Listing
April 2021

Neolinulicin A and B from Inula japonica and their anti-inflammatory activities.

Fitoterapia 2021 Apr 10;152:104905. Epub 2021 Apr 10.

State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Haike Road, Shanghai 201203, People's Republic of China; University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing 100049, People's Republic of China. Electronic address:

A phytochemical study performed on Inula japonica led to isolation of a new 1,10-seco-sesquiterpene dimer Neolinulicin A (1) and 1,10-seco-sesquiterpene Neolinulicin B (2), together with nine known sesquiterpenes (3-11). Among them, Neolinulicin A (1), which has a new carbon skeleton, was a Diels-Alder [4 + 2] adduct of two sesquiterpene moieties. Their structures were established by extensive spectroscopic analysis. All of the isolated compounds showed inhibition of NO production in RAW 264.7 macrophages. The findings might supply information for the future design of anti-inflammatory agents from I. japonica.
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http://dx.doi.org/10.1016/j.fitote.2021.104905DOI Listing
April 2021

Dyskinesia-hyperpyrexia syndrome in Parkinson's disease: a systematic review.

Clin Auton Res 2021 Apr 7. Epub 2021 Apr 7.

Geriatric Neurological Department of the Second Medical Center, National Clinical Research Center for Geriatric Diseases, Chinese People's Liberation Army General Hospital, Beijing, China.

Purpose: Dyskinesia-hyperpyrexia syndrome (DHS) is a rare but life-threatening disease. The clinical manifestations of this syndrome overlap substantially with Parkinson hyperpyrexia syndrome and serotonin syndrome and are often confused by clinicians. The purpose of this review was to enable clinicians to recognize this syndrome and thereby reach a correct diagnosis and provide optimal treatments to improve prognosis in clinical practice.

Methods: Using the methodology described in the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement, we conducted a literature search of the PubMed, Embase, and MEDLINE databases using keywords in titles and abstracts of published literature. Quality assessment was performed using the modified Newcastle-Ottawa scale.

Results: A total of 11 patients obtained from nine publications were included in this systematic review. All of the cases occurred in patients with advanced Parkinson's disease (PD) of long disease duration. High ambient temperature was the most common trigger of this syndrome. Hyperpyrexia and dyskinesias were present in all cases. The consciousness disturbances of this syndrome included confusion, hallucination, and lethargy or stupor. Autonomic dysfunction (except for hyperpyrexia) is uncommon in DHS, and only two patients presented with tachycardia. The treatment of this syndrome included supportive interventions (including rehydration, anti-pyretic and anti-infection treatments, and maintaining electrolyte balance), dopaminergic drug reduction and sedation. Two patients died due to DHS.

Conclusions: We summarized the triggers, clinical features, and treatments of all reported dyskinesia-hyperpyrexia syndrome cases, proposed guiding diagnostic criteria, and established a flow chart to guide diagnoses to quickly identify these three syndromes in clinical practice.
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http://dx.doi.org/10.1007/s10286-021-00801-wDOI Listing
April 2021

MicroRNA-497-5p Is Downregulated in Hepatocellular Carcinoma and Associated with Tumorigenesis and Poor Prognosis in Patients.

Int J Genomics 2021 16;2021:6670390. Epub 2021 Mar 16.

Department of Urology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, China.

Background: MicroRNAs (miRNAs) have been demonstrated to exhibit important regulatory roles in multiple malignancies, including hepatocellular carcinoma (HCC). hsa-miR-497-5p was reported to involve in cancer progression and poor prognosis in many kinds of tumors. However, the expression and its clinical significance of hsa-miR-497-5p in HCC remain unclear.

Methods: In the present study, we investigated the expression of hsa-miR-497-5p in HCC and analyzed the correction of clinical features with prognosis. The expression levels of hsa-miR-497-5p and potential target genes were analyzed in HCC and adjacent noncancerous tissues using The Cancer Genome Atlas (TCGA) database and Gene Expression Omnibus (GEO) datasets. Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to analyze hsa-miR-497-5p levels in 328 HCC tissues and 30 paired adjacent noncancer tissues. Overall survival (OS) and progression-free survival (PFS) of patients with HCC were assessed using the Kaplan-Meier method and the log-rank test.

Results: The hsa-miR-497-5p expression levels were decreased, and its target genes ACTG1, CSNK1D, PPP1CC, and BIRC5 were upregulated in HCC tissues compared with normal tissues. Lower levels of hsa-miR-497-5p expression and higher levels of the four target genes were significantly associated with higher tumor diameter. Moreover, patients with lower hsa-miR-497-5p expression and higher target genes levels had shorter OS.

Conclusion: The expression levels of hsa-miR-497-5p may play an important regulatory role in HCC and are closely correlated with HCC progression and poor prognosis in patients. The hsa-miR-497-5p may be a specific therapeutic target for the treatment of HCC.
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http://dx.doi.org/10.1155/2021/6670390DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7987441PMC
March 2021

Forsythiaside a plays an anti-inflammatory role in LPS-induced mastitis in a mouse model by modulating the MAPK and NF-κB signaling pathways.

Res Vet Sci 2021 May 24;136:390-395. Epub 2021 Mar 24.

Biotechnology Developing Center of Henan Academy of Sciences, Henan Academy of sciences, Zhengzhou 450002, Henan Province, PR China. Electronic address:

Forsythiaside A, a major bioactive component extracted from Forsythiae fructus, possesses multiple biological properties, especially anti-inflammatory properties. In the present study, the anti-inflammatory effect of forsythiaside A was investigated in lipopolysaccharide (LPS)-induced acute mastitis in mice. Our results showed that the expression levels of IL-1β, IL-6, TNF-α, p38 MAPK, IκBα, and NF-κB p65 in the LPS group were all up-regulated, and obvious pathological changes were observed by sectioning. Compared with those in the LPS group, the expression levels of the above factors were significantly reduced, and the inflammation symptoms were also significantly reduced by section observation after forsythiaside A intervention. These results indicated that forsythiaside A effectively inhibited LPS-induced mammary inflammation in mice by attenuating the activation of the NF-κB and p38 MAPK signaling pathways.
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http://dx.doi.org/10.1016/j.rvsc.2021.03.020DOI Listing
May 2021

The complete mitochondrial genome of (Isoptera: Rhinotermitidae).

Mitochondrial DNA B Resour 2021 Mar 11;6(3):794-795. Epub 2021 Mar 11.

College of Agricultural and Food Science, Zhejiang A&F University, Hangzhou, PR China.

The mitochondrial genome of the is 15,913 bp in length and encodes 37 genes including 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNA), 2 ribosomal RNA genes (rRNA), and a non-coding control region (D-loop). The percentage of A/T (65.59%) is much higher than that of C/G (34.41%). The phylogenetic tree revealed that was closest to and . The mitochondrial genome of the provides a resource for evolutional analysis within termites especially .
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http://dx.doi.org/10.1080/23802359.2021.1882903DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7954491PMC
March 2021

Associations of Plasma Glucagon Levels with Estimated Glomerular Filtration Rate, Albuminuria and Diabetic Kidney Disease in Patients with Type 2 Diabetes Mellitus.

Diabetes Metab J 2021 Mar 23. Epub 2021 Mar 23.

Department of Endocrinology, Affiliated Hospital 2 of Nantong University, and First People's Hospital of Nantong City, Nantong, China.

Background: Type 2 diabetes mellitus (T2DM) is characterized by elevated fasting glucagon and impaired suppression of postprandial glucagon secretion, which may participate in diabetic complications. Therefore, we investigated the associations of plasma glucagon with estimated glomerular filtration rate (eGFR), albuminuria and diabetic kidney disease (DKD) in T2DM patients.

Methods: Fasting glucagon and postchallenge glucagon (assessed by area under the glucagon curve [AUCgla]) levels were determined during oral glucose tolerance tests. Patients with an eGFR <60 mL/min/1.73 m2 and/or a urinary albumin-to-creatinine ratio (UACR) ≥30 mg/g who presented with diabetic retinopathy were identified as having DKD.

Results: Of the 2,436 recruited patients, fasting glucagon was correlated with eGFR and UACR (r=-0.112 and r=0.157, respectively; P<0.001), and AUCgla was also correlated with eGFR and UACR (r=-0.267 and r=0.234, respectively; P<0.001). Moreover, 31.7% (n=771) presented with DKD; the prevalence of DKD was 27.3%, 27.6%, 32.5%, and 39.2% in the first (Q1), second (Q2), third (Q3), and fourth quartile (Q4) of fasting glucagon, respectively; and the corresponding prevalence for AUCgla was 25.9%, 22.7%, 33.7%, and 44.4%, respectively. Furthermore, after adjusting for other clinical covariates, the adjusted odds ratios (ORs; 95% confidence intervals) for DKD in Q2, Q3, and Q4 versus Q1 of fasting glucagon were 0.946 (0.697 to 1.284), 1.209 (0.895 to 1.634), and 1.521 (1.129 to 2.049), respectively; the corresponding ORs of AUCgla were 0.825 (0.611 to 1.114), 1.323 (0.989 to 1.769), and 2.066 (1.546 to 2.760), respectively. Additionally, when we restricted our analysis in patients with glycosylated hemoglobin <7.0% (n=471), we found fasting glucagon and AUCgla were still independently associated with DKD.

Conclusion: Both increased fasting and postchallenge glucagon levels were independently associated with DKD in T2DM patients.
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http://dx.doi.org/10.4093/dmj.2020.0149DOI Listing
March 2021

Resistance Training with Blood Flow Restriction on Vascular Function: A Meta-analysis.

Int J Sports Med 2021 Mar 18. Epub 2021 Mar 18.

Department of Marxism Studies, Jiangsu Normal University, Xuzhou, China.

In this meta-analysis, the aims were to examine the effects of resistance training with and without blood flow restriction on vascular function. Five databases were searched up to June 2020 for papers about resistance training with blood flow restriction influence on vascular function. The quality of each identified study was evaluated. Effect sizes were estimated in terms of the standardized mean difference. A subgroup analysis was conducted according to participants' age, training duration, and cuff pressure. The results of six studies on arterial compliance and five on vascular function were highly homogenous regarding responses to resistance training with or without blood flow restriction. Resistance training with blood flow restriction had a more positive effect for regulating arterial compliance than resistance training without blood flow restriction, but not for vascular function. Resistance training with blood flow restriction leads to a more positive effect on vascular function than resistance training when training for no longer than four weeks.
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http://dx.doi.org/10.1055/a-1386-4846DOI Listing
March 2021

Influence of chemical treatment and drying method on the properties of cellulose fibers of luffa sponge.

Int J Biol Macromol 2021 Jun 16;180:112-120. Epub 2021 Mar 16.

College of Forest and Garden, Anhui Agricultural University, Hefei 230036, China. Electronic address:

The exploration of modified luffa sponge (LS) cellulose fiber in the field of polymer composite can contribute to the development of high-performance and lightweight composites. In this study, two chemical treatments (10%NaOH-20%CHCOOH (Method 1) and 10%NaOH-5%NaSO (Method 2)) and two drying methods (air drying and freeze-drying) were used to treat LS. The microscopic characteristics and physical properties showed that Methods 1 and 2 caused shrinkage of the LS fibers and increased their fiber density by 30.6% and 15.0%. Meanwhile, freeze-drying kept the cells of modified LS fibers full and decreased their fiber density by 5.0% and 21.0%, respectively. The tensile properties test analyses indicated that freeze-drying further increased the elongation at break values of modified LS fibers by 25.3% and 17.7%, respectively. The moisture absorption analyses showed that freeze-drying could further decrease the moisture absorption ratios of modified LS fibers by 25.8% and 35.8%, respectively, which was useful for improving the dimensional stability of composite materials. Moreover, the thermogravimetric analysis reveals that freeze-drying increased onset degradation temperatures of the modified fibers by 24.0 °C and 6.7 °C, which was beneficial to improve the thermal stability of the composite material.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.03.053DOI Listing
June 2021

Integrated Protein-Protein Interaction and Weighted Gene Co-expression Network Analysis Uncover Three Key Genes in Hepatoblastoma.

Front Cell Dev Biol 2021 26;9:631982. Epub 2021 Feb 26.

Department of Microbiology, Faculty of Basic Medical Sciences, Guilin Medical University, Guilin, China.

Hepatoblastoma (HB) is the most common liver tumor in the pediatric population, with typically poor outcomes for advanced-stage or chemotherapy-refractory HB patients. The objective of this study was to identify genes involved in HB pathogenesis via microarray analysis and subsequent experimental validation. We identified 856 differentially expressed genes (DEGs) between HB and normal liver tissue based on two publicly available microarray datasets (GSE131329 and GSE75271) after data merging and batch effect correction. Protein-protein interaction (PPI) analysis and weighted gene co-expression network analysis (WGCNA) were conducted to explore HB-related critical modules and hub genes. Subsequently, Gene Ontology (GO) analysis was used to reveal critical biological functions in the initiation and progression of HB. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that genes involved in cell cycle phase transition and the PI3K/AKT signaling were associated with HB. The intersection of hub genes identified by both PPI and WGCNA analyses revealed five potential candidate genes. Based on receiver operating characteristic (ROC) curve analysis and reports in the literature, we selected CCNA2, CDK1, and CDC20 as key genes of interest to validate experimentally. CCNA2, CDK1, or CDC20 small interfering RNA (siRNA) knockdown inhibited aggressive biological properties of both HepG2 and HuH-6 cell lines . In conclusion, we identified CCNA2, CDK1, and CDC20 as new potential therapeutic biomarkers for HB, providing novel insights into important and viable targets in future HB treatment.
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http://dx.doi.org/10.3389/fcell.2021.631982DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7953069PMC
February 2021

CircHIF1A regulated by FUS accelerates triple-negative breast cancer progression by modulating NFIB expression and translocation.

Oncogene 2021 Apr 13;40(15):2756-2771. Epub 2021 Mar 13.

Department of Breast Surgery, General Surgery, Qilu Hospital of Shandong University, Jinan, Shandong, China.

Emerging evidence has demonstrated that circular RNAs (circRNAs) play critical roles in the development and progression of human cancer. However, the biological functions and underlying mechanisms of circRNAs in triple-negative breast cancer (TNBC) remain to be investigated. In our present study, we found that the novel circRNA circHIF1A was significantly overexpressed in breast cancer tissues and that it was associated with metastasis, poor prognosis, and the TNBC subtype. Gain- and loss-of-function experiments were conducted to investigate the biological roles of circHIF1A in TNBC. Overexpression of circHIF1A significantly promoted TNBC growth and metastasis in vitro and in vivo, while knockdown of circHIF1A exerted the opposite effects. Mechanistically, circHIF1A modulated the expression and translocation of NFIB through posttranscriptional and posttranslational modifications, resulting in the activation of the AKT/STAT3 signaling pathway and inhibition of P21. The RNA binding protein FUS could regulate the biogenesis of circHIF1A by interacting with the flanking intron, and FUS was transcriptionally regulated by NFIB, thus forming the circHIF1A/NFIB/FUS positive feedback loop. Moreover, circHIF1A could be packaged into exosomes and was upregulated in the plasma of breast cancer patients. Our findings indicated that circHIF1A played a critical role in the growth and metastasis of TNBC via a positive feedback loop and that circHIF1A could be a promising biomarker for breast cancer diagnosis and a potential therapeutic target for TNBC treatment.
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http://dx.doi.org/10.1038/s41388-021-01739-zDOI Listing
April 2021