Publications by authors named "Tingting Zhou"

291 Publications

The effect of different crystalline phases of InO on the ozone sensing performance.

J Hazard Mater 2021 Jun 2;418:126290. Epub 2021 Jun 2.

State Key Laboratory of Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, Changchun 130012, PR China. Electronic address:

Crystalline phase regulation could optimize the band gap, which has a great impact on the amount of chemisorbed gas molecules on the gas sensing materials. Herein, a facile route of hydrothermal method followed by calcination treatment was used to synthesize cubic bixbyite-type (C-InO), rhombohedral corundum-type (Rh-InO) and the mixed phase InO (Rh+C-InO). The band gap of C-InO was narrowed to a suitable value (2.38 eV) and the relative percentage of chemisorbed oxygen was enhanced (31.8%). The sensing results to ozone (O) indicated that the C-type structure stood out. The gas sensor based on C-InO exhibited extraordinary O sensing performances with a response of 5.7 (100 ppb) and an ultralow limit of detection of 30 ppb. The amazing results could be attributed to the narrow band gap and the enrichment of chemisorbed oxygen. This work inspires a new perspective to design highly sensitive and reliable O sensors.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126290DOI Listing
June 2021

UPLC-MS-Based Serum Metabolomics Reveals Potential Biomarkers of Ang II-Induced Hypertension in Mice.

Front Cardiovasc Med 2021 5;8:683859. Epub 2021 May 5.

Laboratory of Cardiovascular Research, School of Medicine, Jiangnan University, Wuxi, China.

Hypertension is caused by polygenic inheritance and the interaction of various environmental factors. Abnormal function of the renin-angiotensin-aldosterone system (RAAS) is closely associated with changes in blood pressure. As an essential factor in the RAAS, angiotensin II (Ang II) contributes to vasoconstriction and inflammatory responses. However, the effects of overproduction of Ang II on the whole body-metabolism have been unclear. In this study, we established a hypertensive mouse model by micro-osmotic pump perfusion of Ang II, and the maximum systolic blood pressure reached 140 mmHg after 2 weeks. By ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry, the metabolites in the serum of hypertensive model and control mice were analyzed. Partial least squares discriminant analysis (PLS-DA) in both positive and negative ionization modes showed clear separation of the two groups. Perfusion of Ang II induced perturbations of multiple metabolic pathways in mice, such as steroid hormone biosynthesis and galactose metabolism. Tandem mass spectrometry revealed 40 metabolite markers with potential diagnostic value for hypertension. Our data indicate that non-targeted metabolomics can reveal biochemical pathways associated with Ang II-induced hypertension. Although researches about the clinical use of these metabolites as potential biomarkers in hypertension is still needed, the current study improves the understanding of systemic metabolic response to sustained release of Ang II in hypertensive mice, providing a new panel of biomarkers that may be used to predict blood pressure fluctuations in the early stages of hypertension.
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http://dx.doi.org/10.3389/fcvm.2021.683859DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8131677PMC
May 2021

Molecular cloning and expression analysis of a WRKY transcription factor gene, , from .

Plant Signal Behav 2021 May 23:1930442. Epub 2021 May 23.

Co-Innovation Center for Sustainable Forestry in Southern China; College of Forestry, Nanjing Forestry University, Nanjing, China.

WRKY transcription factors are important regulators of diverse plant life processes. Our aim was to clone and characterize , a WRKY gene of group IIc, derived from . The cDNA sequence of was 818 bp long, encoding a 271-amino acid proteins and containing two introns and three exons. The proteinic molecular weight was 30.99 kDa, with a relevant theoretical isoelectric point of 8.15. Subcellular localization analysis confirmed that the protein localized to the nucleus. In total, 75 cis-regulatory elements of 19 different types were identified in the promoter sequence, including some elements involved in light responsiveness, anaerobic induction and circadian control, low-temperature responsiveness, as well as salicylic acid (SA) and auxin responsiveness. Expression pattern analysis of plant samples from different developmental stages and tissue types, revealed differential expression. The transcript was downregulated 12 h after heat treatment and at 4-12 h after drought treatment, but was upregulated 12 h after NaCl, cold and methyl jasmonate treatments. For abscisic acid and SA treatments, the transcript was upregulated at 24 h. In summary, encoded a newly cloned WRKY transcription factor of that might be involved in plant growth and plant responses to abiotic stresses and hormones treatments.
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http://dx.doi.org/10.1080/15592324.2021.1930442DOI Listing
May 2021

An analysis of the dynamic changes in the self-efficacy and quality of life of elderly patients with chronic obstructive pulmonary disease following community-based rehabilitation.

Am J Transl Res 2021 15;13(4):2745-2751. Epub 2021 Apr 15.

Department of Respiration, Nantong Hospital of Traditional Chinese Medicine Nantong 226000, Jiangsu, China.

Objective: This study aimed to investigate the effects of community-based rehabilitation (CBR) on the self-efficacy and quality of life in elderly patients with chronic obstructive pulmonary disease (COPD).

Method: Eighty-one elderly patients with COPD admitted to our hospital were recruited as the study cohort and were randomly divided into a control group (n=41) and a study group (n=40). The control group underwent outpatient rehabilitative treatment, and the study group additionally underwent CBR. The treatment effects of the two groups at 1 month, 3 months, and 6 months of intervention were assessed using their pulmonary function and quality of life scores.

Results: After completion of the CBR, the patients in the study group and the control group were scored using the CSES scale, which did not differ at 1 month of intervention, but the scores were higher in the study group than they were in the control group at 3 and 6 months of intervention ( < 0.05). The patients in the study group also scored higher on the WHOQOL-BREF scale than the control group ( < 0.05).

Conclusion: CBR improves the self-efficacy and quality of life in elderly patients with COPD.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129375PMC
April 2021

Single nucleotide polymorphisms of TRAF2 and TRAF5 gene in ankylosing spondylitis: a case-control study.

Clin Exp Med 2021 May 17. Epub 2021 May 17.

Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, 81 Meishan Road, Hefei, 230032, Anhui, China.

Objective To investigate the role of eight locus polymorphisms of tumor necrosis factor receptor-associated factor 2 (TRAF2) and TRAF5 gene and their interaction in the susceptibility to ankylosing spondylitis (AS) in Chinese Han population. Methods Eight single nucleotide polymorphisms (SNPs) of TRAF2 (rs3750511, rs10781522, rs17250673, rs59471504) and TRAF5 (rs6540679, rs12569232, rs4951523, rs7514863) gene were genotyped in 673 AS patients and 687 controls. Results The SNPs of TRAF2 and TRAF5 do not indicate a correlation with the susceptibility of AS in Chinese Han population. Genotype frequencies of rs3750511 were statistically significant in females between patients and controls. The allele frequencies of rs10781522 and genotype frequencies of rs3750511 were statistically significant between groups of different diseases activity. One three-locus model, TRAF2 (rs10781522, rs17250673) and TRAF5 (rs12569232), had a maximum testing accuracy of 52.67% and a maximum cross-validation consistency (10/10) that was significant at the level of P = 0.0001, after determined empirically by permutation testing. As to environmental variables, only marginal association was found between sleep quality and AS susceptibility. Conclusion TRAF2 rs3750511 polymorphism may be associated with the susceptibility and severity of AS. Besides, the interaction of TRAF2 and TRAF5 genes may be associated with AS susceptibility, but many open questions remain.
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http://dx.doi.org/10.1007/s10238-021-00719-7DOI Listing
May 2021

MicroRNA-370 carried by M2 macrophage-derived exosomes alleviates asthma progression through inhibiting the FGF1/MAPK/STAT1 axis.

Int J Biol Sci 2021 23;17(7):1795-1807. Epub 2021 Apr 23.

Department of Pediatrics, Shengjing Hospital of China Medical University, Shenyang 110004, China.

Emerging evidence has suggested the functions of exosomes in allergic diseases including asthma. By using a mouse model with asthma induced by ovalbumin (OVA), we explored the roles of M2 macrophage-derived exosomes (M2Φ-Exos) in asthma progression. M2Φ-Exos significantly alleviated OVA-induced fibrosis and inflammatory responses in mouse lung tissues, as well as inhibited abnormal proliferation, invasion, and fibrosis-related protein production in platelet derived growth factor (PDGF-BB) treated primary mouse airway smooth muscle cells (ASMCs). The OVA administration in mice or the PDGF-BB treatment in ASMCs reduced the expression of miR-370, which was detected in M2Φ-Exos by miRNA sequencing. However, treating the mice or ASMCs with M2Φ-Exos reversed the inhibitory effect of OVA or PDGF-BB on miR-370 expression. We identified that the target of miR-370 was fibroblast growth factor 1 (). Downregulation of miR-370 by Lv-miR-370 inhibitor or overexpression of FGF1 by Lv-FGF1 blocked the protective roles of M2Φ-Exos in asthma-like mouse and cell models. M2Φ-Exos were found to inactivate the MAPK signaling pathway, which was recovered by miR-370 inhibition or FGF1 overexpression. Collectively, we conclude that M2Φ-Exos carry miR-370 to alleviate asthma progression through downregulating FGF1 expression and the MAPK/STAT1 signaling pathway. Our study may offer a novel insight into asthma treatment.
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http://dx.doi.org/10.7150/ijbs.59715DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120458PMC
April 2021

PGE1 triggers Nrf2/HO-1 signal pathway to resist hemin-induced toxicity in mouse cortical neurons.

Ann Transl Med 2021 Apr;9(8):634

Department of Neurology, Affiliated Hospital of Nantong University, Nantong, China.

Background: Prostaglandin E1 (PGE1) exerts various pharmacological effects such as membrane stabilization, anti-inflammatory functions, vasodilation, and platelet aggregation inhibition. We have previously demonstrated that PGE1 has a beneficial impact on patients suffering from intracerebral hemorrhage (ICH). The related mechanism underlying PGE1's beneficial effect on ICH treatment needs further exploration.

Methods: The present study elucidates the mechanism of PGE1 on ICH treatment using a neuronal apoptosis model . The mouse primary cortical neurons were pretreated with different concentrations of PGE1, followed by the treatment with hemin, the main catabolite in whole blood, to mimic the clinical ICH.

Results: Comparing with the vehicle-treated group, PGE1 prevented cultured cortical neurons from the accumulation of inhibited intracellular levels of reactive oxygen species (ROS), amelioration of mitochondrial membrane potential, and hemin-induced apoptosis. The reduction of ROS and apoptosis were associated with the up-regulation of Heme oxygenase-1 (HO-1) expression. Knockdown of nuclear transcription factor erythroid 2-related factor (Nrf2) by siRNA attenuated the upregulation of HO-1 as well as the protective effect of PGE1.

Conclusions: Our work suggests that the Nrf2/HO-1 molecular pathway may play a crucial role in treating ICH patients with PGE1 and may represent novel molecular targets, resulting in discovering new drugs for ICH treatment.
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http://dx.doi.org/10.21037/atm-20-5839DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106031PMC
April 2021

Role of Transient Receptor Potential Vanilloid 4 in Vascular Function.

Front Mol Biosci 2021 26;8:677661. Epub 2021 Apr 26.

Wuxi School of Medicine, Jiangnan University, Wuxi, China.

Transient receptor potential vanilloid 4 (TRPV4) channels are widely expressed in systemic tissues and can be activated by many stimuli. TRPV4, a Ca-permeable cation channel, plays an important role in the vasculature and is implicated in the regulation of cardiovascular homeostasis processes such as blood pressure, vascular remodeling, and pulmonary hypertension and edema. Within the vasculature, TRPV4 channels are expressed in smooth muscle cells, endothelial cells, and perivascular nerves. The activation of endothelial TRPV4 contributes to vasodilation involving nitric oxide, prostacyclin, and endothelial-derived hyperpolarizing factor pathways. TRPV4 activation also can directly cause vascular smooth muscle cell hyperpolarization and vasodilation. In addition, TRPV4 activation can evoke constriction in some specific vascular beds or under some pathological conditions. TRPV4 participates in the control of vascular permeability and vascular damage, particularly in the lung capillary endothelial barrier and lung injury. It also participates in vascular remodeling regulation mainly by controlling vasculogenesis and arteriogenesis. This review examines the role of TRPV4 in vascular function, particularly in vascular dilation and constriction, vascular permeability, vascular remodeling, and vascular damage, along with possible mechanisms, and discusses the possibility of targeting TRPV4 for therapy.
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http://dx.doi.org/10.3389/fmolb.2021.677661DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107436PMC
April 2021

Metabolomics based comprehensive investigation of Gardeniae Fructus induced hepatotoxicity.

Food Chem Toxicol 2021 Jul 5;153:112250. Epub 2021 May 5.

School of Pharmacy, Second Military Medical University, Shanghai, 200433, China; Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, Shanghai, 200433, China. Electronic address:

Gardeniae Fructus (Zhizi in Chinese, ZZ in brief), a commonly used herbal medicine, has aroused wide concern for hepatotoxicity, but the mechanism remains to be investigated. This study was aimed at investigating the mechanism of ZZ-induced liver injury in vivo and in vitro based on metabolomics and evaluating the hepatotoxicity prediction ability of the in vitro model. SD rats were administered with extracted ZZ and HepG2 cells were treated with genipin, the major hepatotoxic metabolite of ZZ. Liver, plasma, intracellular and extracellular samples were obtained for metabolomics analysis. As a result, ZZ caused plasma biochemical and liver histopathological alterations in rats, and induced purine and amino acid metabolism disorder in the liver and pyrimidine, primary bile acids, amino acid metabolism and pantothenate and CoA biosynthesis disorder in the plasma. Pyrimidine, purine, amino acid metabolism and pantothenate and CoA biosynthesis were also found to be disturbed in the genipin-treated HepG2 cells, which exhibited similarity with the result in vivo. This study comprehensively illustrates the underlying mechanism involved in ZZ-related hepatotoxicity from the aspect of metabolome, and provides evidence that identifying hepatotoxicity can be achieved in cells, representing a non-animal alternative for systemic toxicology.
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http://dx.doi.org/10.1016/j.fct.2021.112250DOI Listing
July 2021

Sb/Pd co-doped SnO2 nanoparticles for methane detection: resistance reduction and sensing performance studies.

Nanotechnology 2021 May 6. Epub 2021 May 6.

State Key Laboratory of Integrated Optoelectronics, Jilin University, Qianwei Campus, 2699 Qianjin Street, Changchun 130012, PEOPLE'S REPUBLIC OF CHINA,, changchun, CHINA.

Methane (CH4) gas sensors play an important role in industrial safety and detection of indoor gas quality. In general, metal oxide semiconductor sensing materials with nano-structure have high responses to the target gas. However, the sensor resistance is usually very high. Considering the practical application, it is vital to reduce base resistance and improve sensitivity for gas sensors. Herein, Pd-doped SnO2 nanoparticles were prepared as the basis material by a simple sol-gel method. In order to adjust the resistance, the pentavalent metal element (Sb) was introduced via a simple doping route. As CH4 sensing layers, the prepared SnO2-sensors doped with Pd and Sb exhibited the most obvious resistance reduction effect. Meantime, excellent sensing performances including high response, fast response/recovery time, excellent reproducibility and great stability were also obtained. In-depth research has shown that the ability to reduce resistance depends on the effective internal doping of cation with high valence. The enhanced sensing capability can be attributed to the "synergistic effects" including catalytic effects of novel metals, increased oxygen vacancies and decreased band gap energy. This work can provide a new opportunity to design metal oxide sensing materials with low resistance and high sensitivity.
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http://dx.doi.org/10.1088/1361-6528/abfe92DOI Listing
May 2021

Testis-sparing surgery in children with testicular tumors: A systematic review and meta-analysis.

Asian J Surg 2021 Apr 21. Epub 2021 Apr 21.

Department of Pediatric Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China. Electronic address:

Testis-sparing surgery (TSS) has been increasingly used for treating a variety of testicular tumors (TTs) in children. However, the indications and feasibility associated with TSS remain uncertain. This study aimed to present the clinical outcomes of TSS in children with TTs. The PubMed, Cochrane Library, and Embase databases were reviewed for relevant articles on the clinical outcomes of TSS in children. Recurrence rate, benign rate, rate of TSS and its 95% confidence interval (CI) were calculated. A total of nine relevant studies with 320 patients were included in this study. The recurrence rate was 5.8% (95% CI: 2.3%-14.1%), benign rate was 70.9% (95% CI: 56.3%-82.1%), the rate of TSS (RTSS) was 36.2% (95% CI: 26.1%-47.8%), RTSS in benign tumor was 48.4% (95% CI: 34.3%-62.9%) and rate of elevated AFP was 29.3% (95% CI: 19.7%-41.3%) in children with TTs. Regarding the distribution of TTs, 159 (49.6%) were teratomas, 74 (23.1%) were yolk sac tumors, 36 (11.3%) were epidermoid cysts, 3 (0.9%) were rhabdomyosarcomas, 7 (2.2%) were leydig cell tumor, 6 (1.8%) were sex-cord stromal tumor, 8(2.5%) were mixed malignant germ cell tumors, 3(0.9%) were hemangioma, and 4(1.3%) were adrenal rest tumors. The findings of this meta-analysis suggested that most of the TTs in children were benign, and the most common histologic subtype was teratoma. TSS should be provided to children with benign lesions. This study confirmed that very low rates of tumor recurrence were observed in children with TTs.
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http://dx.doi.org/10.1016/j.asjsur.2021.03.016DOI Listing
April 2021

Association of CTLA-4 (+49 A/G) polymorphism with susceptibility to autoimmune diseases: A meta-analysis with trial sequential analysis.

Int Immunopharmacol 2021 Apr 16;96:107617. Epub 2021 Apr 16.

Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, Hefei, Anhui, China; Inflammation and Immune Mediated Diseases Laboratory of Anhui Province, Hefei, Anhui, China. Electronic address:

Objectives: In recent years, more and more studies have been focusing on the association between Cytotoxic T lymphocyte antigen-4 (CTLA-4) (+49 A/G) gene polymorphism and autoimmune diseases. However, the results of previous studies are still controversial. The meta-analysis is aiming at determining the association in CTLA-4 (+49 A/G) gene rs231775 polymorphism and ankylosing spondylitis (AS), rheumatoid arthritis (RA), systemic lupus erythematosus (SLE).

Methods: We searched PubMed, Web of Science, Chinese National Knowledge Infrastructure (CNKI) and Chinese Biomedical Database (CBM) up to November 2020, use random or fixed-effect models to perform meta-analysis to compare alleles and other genetic models, including homozygous, heterozygous, recessive and dominant models. The odds ratio (OR) with a 95% confidence interval (95% CI) was used to assess the correlation between CTLA-4 (+49 A/G) gene polymorphism and the genetic affectability of AS, RA, and SLE. Meanwhile, we used sequential trial analysis (TSA) to analyze the reliability of the results. Finally, we searched the relevant data of genome-wide association studies (GWAS) to further verify the accuracy of the experimental results.

Results: 47 studies with 11,893 cases and 12,032 healthy controls were included. The rs231775 G allele was relevant to high risk of autoimmune disease over all people (P < 0.05). The G allele of rs231775 was significantly related to RA susceptibility (P < 0.05), but not with AS or SLE. Subgroup analysis by ethnicity indicated that rs231775 G allele was closely related to RA in Caucasian populations and Mongolian populations (P < 0.05). A strong connection within rs231775 G allele and AS affectability was uncovered in Caucasian populations (P < 0.05). The analysis of the TSA shows that the meta-analysis can draw the conclusion.

Conclusion: CTLA-4 (+49 A/G) gene rs231775 G allele increases the risk of autoimmune diseases in Caucasian populations. And it also increases the risk of RA in Caucasian and Mongolian populations. More sample size and more elaborately designed studies are needed to elucidate the relationship in CTLA-4 (+49 A/G) gene rs231775 G allele and autoimmune diseases, especially AS, SLE.
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http://dx.doi.org/10.1016/j.intimp.2021.107617DOI Listing
April 2021

Connecting the dots: Targeting the microbiome in drug toxicity.

Med Res Rev 2021 Apr 15. Epub 2021 Apr 15.

Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai, China.

The gut microbiota has a vast influence on human health and its role in initiating, aggravating, or ameliorating diseases is beginning to emerge. Recently, its contribution to heterogeneous toxicological responses is also gaining attention, especially in drug-induced toxicity. Whether they are orally administered or not, drugs may interact with the gut microbiota directly or indirectly, which leads to altered toxicity. Present studies focus more on the unidirectional influence of how xenobiotics disturb intestinal microbial composition and functions, and thus induce altered homeostasis. However, interactions between the gut microbiota and xenobiotics are bidirectional and the impact of the gut microbiota on xenobiotics, especially on drugs, should not be neglected. Thus, in this review, we focus on how the gut microbiota modulates drug toxicity by highlighting the microbiome, microbial enzyme, and microbial metabolites. We connect the dots between drugs, the microbiome, microbial enzymes or metabolites, drug metabolites, and host toxicological responses to facilitate the discovery of microbial targets and mechanisms associated with drug toxicity. Besides this, current mainstream strategies to manipulate drug toxicity by targeting the microbiome are summarized and discussed. The review provides technical reference for the evaluation of medicinal properties in the research and development of innovative drugs, and for the future exploitation of strategies to reduce drug toxicity by targeting the microbiome.
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http://dx.doi.org/10.1002/med.21805DOI Listing
April 2021

A Rapid Screening Workflow to Identify Potential Combination Therapy for GBM using Patient-Derived Glioma Stem Cells.

J Vis Exp 2021 03 28(169). Epub 2021 Mar 28.

Department of Cell Biology, School of Basic Medical Sciences, Nanjing Medical University; Institute for Brain Tumors & Key Laboratory of Rare Metabolic Diseases, Nanjing Medical University; Nanjing Medical University Affiliated Cancer Hospital; Key Laboratory of Human Functional Genomics of Jiangsu Province;

The glioma stem cells (GSCs) are a small fraction of cancer cells which play essential roles in tumor initiation, angiogenesis, and drug resistance in glioblastoma (GBM), the most prevalent and devastating primary brain tumor. The presence of GSCs makes the GBM very refractory to most of individual targeted agents, so high-throughput screening methods are required to identify potential effective combination therapeutics. The protocol describes a simple workflow to enable rapid screening for potential combination therapy with synergistic interaction. The general steps of this workflow consist of establishing luciferase-tagged GSCs, preparing matrigel coated plates, combination drug screening, analyzing, and validating the results.
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http://dx.doi.org/10.3791/62312DOI Listing
March 2021

Effects of PGT-A on Pregnancy Outcomes for Young Women Having One Previous Miscarriage with Genetically Abnormal Products of Conception.

Reprod Sci 2021 Mar 15. Epub 2021 Mar 15.

Center for Reproductive Medicine, Shandong University, 157 Jingliu Road, Jinan, 250012, Shandong, China.

In this retrospective study, the effect of preimplantation genetic testing for aneuploidy (PGT-A) was evaluated in women younger than 38 years with a history of one prior miscarriage and embryonic chromosomal abnormalities were detected in previous products of conception (POCs). Abnormal karyotypes were detected in POCs at our center between January 2014 and December 2017. Of the women included in this analysis, 124 continued with conventional in vitro fertilization/intracytoplasmic sperm injection cycles (non-PGT-A group) and 93 chose PGT-A cycles (PGT-A group), and the pregnancy outcomes in both groups were compared. Although the clinical pregnancy rate per embryo transfer was significantly higher in the PGT-A group (67.23% vs. 51.85%, p-adj = 0.01), no between-group differences were found in the live birth rate or miscarriage rate (45.38% vs. 40.74%, p-adj = 0.59; 16.25% vs. 14.29%, p-adj = 0.15). Women in both groups had comparative cumulative live birth rates (PGT-A vs. non-PGT-A, 58.06% vs. 53.23%, p = 0.48). The main results of this study suggest that PGT-A is not associated with an increased likelihood of a live birth or a decreased rate of miscarriage among women younger than 38 years without recurrent pregnancy loss and with a history of POCs with embryonic chromosomal abnormalities.
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http://dx.doi.org/10.1007/s43032-021-00542-1DOI Listing
March 2021

The role of IFT140 in early bone healing of tooth extraction sockets.

Oral Dis 2021 Mar 7. Epub 2021 Mar 7.

Department of Implantology, School & Hospital of Stomatology, Tongji University, Shanghai, China.

Objectives: Primary cilium is a key organelle of regulating bone development and maintenance. The aim of this study is to investigate whether ciliary intraflagellar transporter protein 140 (IFT140) plays a positive role in extraction socket healing by promoting bone formation.

Materials And Methods: A left maxillary first molar extraction model was established using 6-week-old Ift140 (Ctrl group) and Ift140 , Osx-cre (cKO group) mice. The maxillary bone samples from 1, 2, and 3 weeks were postoperatively evaluated by micro-CT, molecular biology, and histomorphometry analysis. Alveolar bone marrow stromal cells (aBMSCs) from 4-week-old mice were cultured in vitro and tested for proliferation and osteogenic ability.

Results: Ciliated cells were predominantly observed in the early socket healing stage with highly expressed ciliary protein IFT140. Compared with the Ctrl group, the healing of extraction sockets in the cKO group was significantly delayed. The proliferation and osteogenic differentiation ability of aBMSCs were reduced in the cKO group.

Conclusion: IFT140 has a facilitating role in the early osteogenesis of extraction socket healing and is involved in regulating the proliferation and osteogenic differentiation of aBMSCs.
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http://dx.doi.org/10.1111/odi.13833DOI Listing
March 2021

Insulin-like androgenic gland hormone from the shrimp Fenneropenaeus merguiensis: Expression, gene organization and transcript variants.

Gene 2021 May 23;782:145529. Epub 2021 Feb 23.

Fisheries College, Guangdong Ocean University, Zhanjiang, Guangdong, PR China. Electronic address:

Male sex differentiation in the crustacean is best known to be controlled by the insulin-like androgenic gland hormone (IAG). In this report, the cDNA and gene of the shrimp Fenneropenaeus merguiensis FmIAG were studied and characterized. FmIAG gene shares a high sequence identity in the coding region as well as the promoter region with that of F. chinensis. FmIAG gene is most likely consists of 5 exons and 4 introns. The cDNA reported here is the most abundant transcript that retained cryptic intron 4. The use of different splicing acceptor sites in exon 2 can produce a long-form FmIAG transcript variant with 6 additional amino acids inserted. Splicing of cryptic intron 4 would produce a transcript variant with a different C-terminal end. Therefore 4 different FmIAG transcripts can be produced from the FmIAG gene. During the molt cycle, the expression level of FmIAG was low in the early intermolt, increase steadily towards the late premolt and decreased rapidly in the early postmolt. In addition to the androgenic gland, FmIAG is also expressed in the hepatopancreas and ovary of adult females. Unilateral eyestalk ablation caused a significant increase in FmIAG transcript suggesting that the eyestalk consists of inhibiting factor(s) that suppressesFmIAGexpression. To explore the function of FmIAG in males, injection of FmIAG dsRNA knock-down the expression of FmIAG and up-regulated the expression of the vitellogenin gene in the testis and hepatopancreas. Interestingly a CHH-like gene identified in the androgenic gland was down-regulated. CHH-like gene knock-down resulted in altered expression of FmIAG in males suggesting that the CHH-like may be involved in FmIAG's regulation. RT-PCR with specific primers to the different transcript variant were used to determine if there is an association of different sizes of male and the type of IAG transcript. Results indicated that a high percentage of the large male shrimp expressed the long-form of FmIAG. The results suggested that FmIAG may be useful as a size marker for male shrimp aquaculture. In summary, the results of this study have expanded our knowledge of shrimp insulin-like androgenic gland hormone in male sex development and its potential role as a marker gene for growth regulation in shrimp.
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http://dx.doi.org/10.1016/j.gene.2021.145529DOI Listing
May 2021

The deacetylation-phosphorylation regulation of SIRT2-SMC1A axis as a mechanism of antimitotic catastrophe in early tumorigenesis.

Sci Adv 2021 Feb 24;7(9). Epub 2021 Feb 24.

Department of Pathology, School of Medicine, University of Alabama at Birmingham, Birmingham, AL 35249-7331, USA.

Improper distribution of chromosomes during mitosis can contribute to malignant transformation. Higher eukaryotes have evolved a mitotic catastrophe mechanism for eliminating mitosis-incompetent cells; however, the signaling cascade and its epigenetic regulation are poorly understood. Our analyses of human cancerous tissue revealed that the NAD-dependent deacetylase SIRT2 is up-regulated in early-stage carcinomas of various organs. Mass spectrometry analysis revealed that SIRT2 interacts with and deacetylates the structural maintenance of chromosomes protein 1 (SMC1A), which then promotes SMC1A phosphorylation to properly drive mitosis. We have further demonstrated that inhibition of SIRT2 activity or continuously increasing SMC1A-K579 acetylation causes abnormal chromosome segregation, which, in turn, induces mitotic catastrophe in cancer cells and enhances their vulnerability to chemotherapeutic agents. These findings suggest that regulation of the SIRT2-SMC1A axis through deacetylation-phosphorylation permits escape from mitotic catastrophe, thus allowing early precursor lesions to overcome oncogenic stress.
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http://dx.doi.org/10.1126/sciadv.abe5518DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7904255PMC
February 2021

Loss of FGFR3 Delays Acute Myeloid Leukemogenesis by Programming Weakly Pathogenic CD117-Positive Leukemia Stem-Like Cells.

Front Pharmacol 2020 29;11:632809. Epub 2021 Jan 29.

Department of Biotechnology, Guangdong Medical University, Dongguan, China.

Chemotherapeutic patients with leukemia often relapse and produce drug resistance due to the existence of leukemia stem cells (LSCs). Fibroblast growth factor receptor 3 (FGFR3) signaling mediates the drug resistance of LSCs in chronic myeloid leukemia (CML). However, the function of FGFR3 in acute myeloid leukemia (AML) is less understood. Here, we identified that the loss of FGFR3 reprograms MLL-AF9 (MA)-driven murine AML cells into weakly pathogenic CD117-positive leukemia stem-like cells by activating the FGFR1-ERG signaling pathway. FGFR3 deletion significantly inhibits AML cells engraftment and extends the survival time of leukemic mice. FGFR3 deletion sharply decreased the expression of chemokines and the prolonged survival time in mice receiving FGFR3-deficient MA cells could be neutralized by overexpression of CCL3. Here we firstly found that FGFR3 had a novel regulatory mechanism for the stemness of LSCs in AML, and provided a promising anti-leukemia approach by interrupting FGFR3.
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http://dx.doi.org/10.3389/fphar.2020.632809DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7879375PMC
January 2021

Predicting a Potential Link to Antidepressant Effect: Neuroprotection of Zhi-zi-chi Decoction on Glutamate-induced Cytotoxicity in PC12 Cells.

Front Pharmacol 2020 25;11:625108. Epub 2021 Jan 25.

School of Pharmacy, Second Military Medical University, Shanghai, China.

Zhi-zi-chi Decoction (ZZCD), composed of (Zhizi in Chinese, ZZ in brief) and (Dandouchi in Chinese, DDC in brief), has been used as a drug therapy for depression for thousands of years in China. However, the antidepressant mechanism of ZZCD still remains unknown. This study was aimed at exploring antidepressant effects of ZZCD from the aspect of neuroprotection based on herb compatibility. Glutamate-treated PC12 cells and chronic unpredictable mild stress (CUMS)-induced rats were established as models of depression and respectively. Cell viability, lactate dehydrogenase (LDH), apoptosis rate, reactive oxygen species (ROS), glutathione reductase (GR) and superoxide dismutase (SOD), and the expressions of Bax, Bcl-2 and cyclic adenosine monophosphate-response element binding protein (CREB) were measured to compare neuroprotection among single herbs and the formula . Behavior tests were conducted to validate antidepressant effects of ZZCD . Results showed that the compatibility of ZZ and DDC increased cell viability and activities of GR and SOD, and decreased the levels of LDH, apoptosis cells and ROS. Besides, the expressions of Bcl-2 and CREB were up-regulated while that of Bax was down-regulated by ZZCD. Furthermore, the compatibility of ZZ and DDC reversed abnormal behaviors in CUMS-induced rats and displayed higher efficacy than any of the single herbs. This study revealed that the antidepressant effects of ZZCD were closely associated with neuroprotection and elucidated synergistic effects of the compatibility of ZZ and DDC based on it.
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http://dx.doi.org/10.3389/fphar.2020.625108DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7868552PMC
January 2021

Effect of the Characteristic Size and Content of Graphene on the Crack Propagation Path of Alumina/Graphene Composite Ceramics.

Materials (Basel) 2021 Jan 28;14(3). Epub 2021 Jan 28.

School of Mechanical and Automotive Engineering, Qilu University of Technology (Shandong Academyof Sciences), Jinan 250353, China.

In this paper, the Voronoimosaic model and the cohesive element method were used to simulate crack propagation in the microstructure of alumina/graphene composite ceramic tool materials. The effects of graphene characteristic size and volume content on the crack propagation behavior of microstructure model of alumina/graphene composite ceramics under different interfacial bonding strength were studied. When the phase interface is weak, the average energy release rate is the highest as the short diameter of graphene is 10-50 nm and the long diameter is 1600-2000 nm. When the phase interface is strong, the average energy release rate is the highest as the short diameter of graphene is 50-100 nm and the long diameter is 800-1200 nm. When the volume content of graphene is 0.50 vol.%, the average energy release rate reaches the maximum. When the velocity load is 0.005 m s, the simulation result is convergent. It is proven that the simulation results are in good agreement with the experimental phenomena.
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http://dx.doi.org/10.3390/ma14030611DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7865948PMC
January 2021

Effects of srtA variation on phagocytosis resistance and immune response of Streptococcus equi.

Infect Genet Evol 2021 Apr 24;89:104732. Epub 2021 Jan 24.

Department of Microbiology and Immunology, College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, Xinjiang, China. Electronic address:

Strangles, which is caused by Streptococcus equi subspecies equi (S. equi), is one of the most prevalent equine infectious diseases with worldwide distribution and leads to serious economic loss in the horse industry. Sortase A (srtA) is a transpeptidase that anchors multiple virulence-associated surface proteins to the cell surface of S. equi. srtA plays a major role in S. equi infection and colonization of the host cell. In this study, we aimed to investigate the effects of srtA mutation on the phagocytic activity and immunogenicity of S. equi. The point-mutated recombinant sortases, including srtA-HT1112 (I88V), srtA-5012 (R147G), and srtA-ZZM17 (control), were expressed, purified, and used to immunize the mouse models. Phagocytic activity was assessed using equine polymorphonuclear cells, whereas opsonophagocytic function and adherence inhibition were measured using the antiserum of these mutants. Mouse serum antibody, bacterial load, and weight gain were also measured. The srtA-HT1112 (I88V) mutant showed significantly enhanced antiphagocytic capability, and its antiserum exhibited increased adherence inhibition activity. In addition, the srtA-HT1112 (I88V) mutant presented the highest lung bacterial load and lowest protection rate (50%) after the challenge with S. equi ZZM17. The srtA-5012 (R147G) mutant exhibited a high IgG2a level and protection rate (62.5%-75%) and the lowest lung bacterial load. These results indicate that the I88V mutation is associated with a high antiphagocytic activity, whereas R147G mutation is associated with the decreased lung bacterial load. Our findings may be useful for the evaluation and development of vaccines.
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http://dx.doi.org/10.1016/j.meegid.2021.104732DOI Listing
April 2021

Enhanced removal of toxic hexavalent chromium from aqueous solution by magnetic [email protected]: performance and mechanism.

Environ Sci Pollut Res Int 2021 Mar 25;28(11):13084-13096. Epub 2021 Jan 25.

Chongqing Key Laboratory of Environmental Materials & Remediation Technologies, Chongqing University of Art and Science, Chongqing, 402160, People's Republic of China.

Magnetic Zr-based metal organic framework (UiO-66) @Polypyrrole (magnetic [email protected]) was prepared to eliminate Cr(VI) from water. SEM and TEM results clearly revealed that the magnetic [email protected] was a core-double-shell structure with the core of FeO, inner shell UiO-66, and outer shell Ppy. The introduction of zirconium MOFs UiO-66 effectively prevented the agglomeration of polypyrrole and provided more available adsorption sites, the surface area increased from 9.57 m/g (Ppy) to 10.57 m/g ([email protected]) and 52.49 m/g (magnetic [email protected]). The magnetic [email protected] possessed a high adsorption capacity of 259.1 mg/g in removing Cr(VI) from water. Adsorption kinetics followed the pseudo-second-order model. The removal of Cr(VI) involved the following mechanisms: (1) electrostatic attraction and ions exchange, the HCrO was adsorbed on the surface of magnetic [email protected] by the protonated N(PpyN) and Cl; (2) reduction, Cr(VI) was reduced to Cr(III) by the reductive functional group(-NH-); (3) chelation, Cr(III) was immobilized on adsorbent by amine groups.
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http://dx.doi.org/10.1007/s11356-021-12341-xDOI Listing
March 2021

Comparison of the overall survival of proximal and distal gastric cancer after gastrectomy: a systematic review and meta-analysis.

World J Surg Oncol 2021 Jan 19;19(1):17. Epub 2021 Jan 19.

Department of Gastroenterology Surgery, The Dalian Municipal Central Hospital Affiliated of Dalian Medical University, No. 826 Southwest Road Shahekou District, Dalian, 116033, P.R. China.

Background: The aim of this study was to investigate the overall survival (OS) between proximal gastric cancer (PG) and distal gastric cancer (DG) patients after gastrectomy.

Methods: Articles on the prognostic study of PG and DG patients after gastrectomy were collected from the PubMed, EMBASE, Web of Science, Cochrane Library, China National Knowledge Infrastructure (CNKI), Wanfang, and VIP databases from the date of establishment until December 2020. The data were statistically analyzed by Stata software (version 11.0, StataCorp).

Results: A total of 10 articles met the inclusion criteria. Meta-analysis showed that the 1-, 3- and 5-year OS rates of PG patients were significantly lower than those of DG patients (RR = 0.898, 95% CI: 0.825 to 0.977, P = 0.013; RR = 0.802, 95% CI: 0.708 to 0.909, P = 0.001; RR = 0.736, 95% CI: 0.642 to 0.844, P = 0.000). After subgroup analysis according to different countries, the combined RR values of were as follows: 1-year OS: eastern countries: RR = 0.966, 95% CI: 0.944 to 0.988, P = 0.003, western countries: RR = 0.687, 95% CI: 0.622 to 0.759, P = 0.000; 3-year OS: eastern countries: RR = 0.846, 95% CI: 0.771 to 0.929, P = 0.000, western countries: RR = 0.742, 95% CI: 0.399 to 1.382, P = 0.348; and 5-year OS: eastern countries: RR = 0.798, 95% CI: 0.716 to 0.889, P = 0.000, western countries: RR = 0.646, 95% CI: 0.414 to 1.008, P = 0.054.

Conclusion: In terms of 1-, 3-, and 5-year OS, PG patients had lower rates than DG patients and the eastern countries/western countries subgroup, but there were no significant differences in 3- and 5-year OS for the western countries. These results merit further clinical validation in future studies. (Registration ID: UMIN000040393; Date of registration: 2020/05/13).
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http://dx.doi.org/10.1186/s12957-021-02126-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816301PMC
January 2021

Systematic investigation and expression profiles of the GbR2R3-MYB transcription factor family in ginkgo (Ginkgo biloba L.).

Int J Biol Macromol 2021 Mar 12;172:250-262. Epub 2021 Jan 12.

Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, PR China. Electronic address:

As one of the largest families of transcription factors, the R2R3-MYB family plays a significant role in plant growth, development, and response to hormone and environmental stress. To explore its evolutionary mechanism and potential function in Ginkgo biloba, a gymnosperm of great economic and ecological value, we presented a comprehensive analysis of the R2R3-MYB genes in ginkgo. Sixty-nine GbR2R3-MYB genes were identified and these genes could be classified into 33 groups based on the characteristics of the amino acid sequence of the R2R3-MYB domain and gene structure. Syntenic analyses indicated that few tandem and segmental duplications possibly resulted in the contraction of the GbR2R3-MYB gene family. Based on the transcriptome data, expression profiles of eight different tissues and different developmental stages of leaf and kernel showed that GbR2R3-MYB genes had distinct temporal and spatial expression characteristics. Specific expression patterns of the sixteen GbR2R3-MYB genes were also identified in response to different abiotic stresses and hormonal exposures. Further investigation revealed that GbR2R3-MYB19 was located in the nucleus and possessed transcriptional activity, implying its potential roles in the regulation of multiple biological processes. Our findings provide a robust basis for future comprehensive evolutionary and functional analyses of GbR2R3-MYB genes in ginkgo.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.01.053DOI Listing
March 2021

Dermal toxicity, dermal irritation, and delayed contact sensitization evaluation of oil body linked oleosin-hEGF microgel emulsion transdermal drug delivery for wound healing.

Cutan Ocul Toxicol 2021 Mar 25;40(1):45-53. Epub 2021 Jan 25.

College of Life Science, Engineering Research Center of the Chinese Ministry of Education for Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, PR China.

The expression of therapeutic proteins in plant oil body bioreactors has attracted much attention. But its safety is not yet clear. This article determines the risk of safety after using the drug. The oil body-linked oleosin-hEGF microgel emulsion (OBEME) was prepared by mixing the xanthan gum with suitable concentrations in an appropriate proportion. Skin irritation and sensitization reaction were investigated in rats and guinea pigs using OBEME as test article. The OBEME did not produce dermal erythema/eschar or oedema responses. The dermal subacute and subchronic toxicity of OBEME were evaluated in accordance with OECD guidelines. Compared with the control group, the basic physical signs, such as weight, feed, drinking, excretion, and behaviour of experimental animals, were not abnormal. In addition, no abnormality was found in haematological parameters, biochemical indexes, relative organ weight, and histopathological observation of organs, and there was no significant difference compared with normal saline treatment group. Therefore, we conclude that OBEME has no toxic effects and is safe and reliable to be used for topical application.
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http://dx.doi.org/10.1080/15569527.2021.1874008DOI Listing
March 2021

Isoflavones' effects on pharmacokinetic profiles of main iridoids from in rats.

J Pharm Anal 2020 Dec 14;10(6):571-580. Epub 2019 Nov 14.

School of Pharmacy, Second Military Medical University, Shanghai, 200433, China.

(GF) and Praeparatum (SSP) are both medicine food homologies and widely used in Chinese clinical prescriptions together. The research investigated the pharmacokinetics of four iridoids in normal rats and isolfavones-fed rats, which were administered with isolfavones from SSP for 7, 14, 21 and 28 consecutive days. A validated LC-MS/MS method was developed for determining shanzhiside, genipin-1-gentiobioside, geniposide and their metabolite genipin in rat plasma. Plasma samples were pretreated by solid-phase extraction using paeoniflorin as the internal standard. The chromatographic separation was performed on a Waters Atlantis T3 (4.6 mm × 150 mm, 3 μm) column using a gradient mobile phase consisting of acetonitril and water (containing 0.06% acetic acid). The mass detection was under the multiple reaction monitoring (MRM) mode via polarity switching between negative and positive ionization modes. The calibration curves exhibited good linearity ( > 0.997) for all components. The lower limit of quantitation was in the range of 1-10 ng/mL. The intra-day and inter-day precisions (RSD) at three different levels were both less than 12.2% and the accuracies (RE) ranged from -10.1% to 16.4%. The extraction recovery of them ranged from 53.8% to 99.7%. Pharmacokinetic results indicated the bioavailability of three iridoid glycosides and the metabolite, genipin in normal rats was higher than that in rats exposed to isoflavones. With the longer time of administration of isoflavones, plasma concentrations of iridoids decreased, while genipin sulfate, the phase Ⅱ metabolite of genposide and genipin-1-gentiobioside, appeared the rising exposure. The pharmacokinetic profiles of main iridoids from GF were altered by isoflavones.
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http://dx.doi.org/10.1016/j.jpha.2019.11.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7775847PMC
December 2020

Molecular characterization and expression dynamics of three key genes in the PI3K-AKT pathway reveal its involvement in the immunotoxicological responses of the giant river prawn Macrobrachium rosenbergii to acute ammonia and nitrite stress.

Ecotoxicol Environ Saf 2021 Jan 11;208:111767. Epub 2020 Dec 11.

College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong Province, PR China; Guangdong Provincial Engineering Laboratory for Mariculture Organism Breeding, Guangdong Ocean University, Zhanjiang, Guangdong Province, PR China; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Guangdong Province, PR China. Electronic address:

Ammonia nitrogen and nitrite are two common forms of environmental toxicants for aquatic organisms including crustaceans. The PI3K-AKT pathway is an important intracellular signaling pathway related to cellular stress response, but involvement of this pathway in the immunotoxicological response of decapod crustaceans to aquatic toxicants such as ammonia nitrogen and nitrite still remains enigmatic. In this study, based on transcriptome mining and molecular cloning techniques, three key genes (named as MrPI3K, MrAKT and MrFoxO) in the PI3K-AKT signaling pathway were identified from the giant river prawn Macrobrachium rosenbergii. Sequence homology and phylogenetic analysis revealed that all the three genes harbored signature sequences of corresponding protein families, and shared high levels of similarities with their respective homologs from other species. MrPI3K, MrAKT and MrFoxO all displayed ubiquitous tissue distribution profiles, but their expression levels varied to a great extend among different tissues and between sexes. Following exposure to nitrite (20 mg/L nitrite-N) or ammonia (25 mg/L total ammonia-N) stresses for 24 h and 48 h, the three genes all responded by altering their expression levels at different time points, but they didn't show uniform expression patterns following these stresses, indicating the diversified roles of these genes in different tissues and the complexity of this signaling pathway. Remarkably, MrPI3K and MrAKT were induced only in the hemocytes and intestine, respectively, indicating their specific roles in these organs. Our study demonstrated the potential utility of these genes as biomarkers of acute ammonia or nitrite toxicity in prawns, and also provided evidence that the PI3K-AKT pathway is involved in the immunotoxicological responses to nitrite and ammonia stress in M. rosenbergii.
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http://dx.doi.org/10.1016/j.ecoenv.2020.111767DOI Listing
January 2021

The Regulatory Effect of SIRT1 on Extracellular Microenvironment Remodeling.

Int J Biol Sci 2021 1;17(1):89-96. Epub 2021 Jan 1.

College of Basic Medical Science, Institute of Translational Medicine, Key Laboratory of Medical Cell Biology, Ministry of Education, Key Laboratory of Liaoning Province, China Medical University, Shenyang, Liaoning Province, P.R. China, 110122.

The sirtuins family is well known by its unique nicotinamide adenine dinucleotide (NAD)-dependent deacetylase function. The most-investigated member of the family, Sirtuin 1 (SIRT1), accounts for deacetylating a broad range of transcription factors and coregulators, such as p53, the Forkhead box O (FOXO), and so on. It serves as a pivotal regulator in various intracellular biological processes, including energy metabolism, DNA damage response, genome stability maintenance and tumorigenesis. Although the most attention has been focused on its intracellular functions, the regulatory effect on extracellular microenvironment remodeling of SIRT1 has been recognized by researchers recently. SIRT1 can regulate cell secretion process and participate in glucose metabolism, neuroendocrine function, inflammation and tumorigenesis. Here, we review the advances in the understanding of SIRT1 on remodeling the extracellular microenvironment, which may provide new ideas for pathogenesis investigation and guidance for clinical treatment.
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http://dx.doi.org/10.7150/ijbs.52619DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757024PMC
January 2021