Publications by authors named "Tingting Zhang"

947 Publications

GPER mediates estrogen cardioprotection against epinephrine-induced stress.

J Endocrinol 2021 Apr 1. Epub 2021 Apr 1.

H Sun, physiology department, Xuzhou Medical University, Xuzhou, China.

Currently, there are no conventional treatments for stress-induced cardiomyopathy (SCM, also known as Takotsubo syndrome), and the existing therapies are not effective. The recently discovered G protein- coupled estrogen receptor (GPER) executes the rapid effects of estrogen (E2). In this study, we investigated the effects and mechanism of GPER on epinephrine (Epi)-induced cardiac stress. SCM was developed with a high dose of Epi in adult rats and human-induced pluripotent stem cells-derived cardiomyocytes(hiPSC-CMs). (1) GPER activation with agonist G1/ E2 prevented an increase in left ventricular internal diameter at end-systole, the decrease both in ejection fraction and cardiomyocyte shortening amplitude elicited by Epi. (2) G1/ E2 mitigated heart injury induced by Epi, as revealed by reduced plasma brain natriuretic peptide and lactate dehydrogenase release into culture supernatant. (3) G1/E2 prevented the raised phosphorylation and internalization of β2-adrenergic receptors(β2AR). (4) Blocking Gαi abolished the cardiomyocyte contractile inhibition by Epi. G1/E2 downregulated Gαi activity of cardiomyocytes and further upregulated cyclic adenosine monophosphate concentration in culture supernatant treated with Epi. (5) G1/E2 rescued decreased Ca2+ amplitude and Ca2+ channel current (ICa-L) in rat cardiomyocytes. Notably, the above effects of E2 were blocked by the GPER antagonist, G15. In hiPSC-CM (which expressed GPER, β1AR and β2ARs), knockdown of GPER by siRNA abolished E2 effects on increasing ICa-L and action potential duration in the stress state. In conclusion, GPER played a protective role against SCM. Mechanistically, this effect was mediated by balancing the coupling of β2AR to the Gαs and Gαi signalling pathways.
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http://dx.doi.org/10.1530/JOE-20-0451DOI Listing
April 2021

Subsoiling increases aggregate-associated organic carbon, dry matter, and maize yield on the North China Plain.

PeerJ 2021 24;9:e11099. Epub 2021 Mar 24.

College of Agronomy, Shandong Agricultural University, Tai'an, Shandong Province, P.R. China.

Background: Soil degradation is one of the main problems in agricultural production and leads to decreases in soil quality and productivity. Improper farming practices speed this process and are therefore not conducive to food security. The North China Plain (NCP) is a key agricultural area that greatly influences food security in China. To explore the effects of different tillage measures on aggregate-associated organic carbon (AOC), the accumulation and transport of dry matter, and maize yield, and to identify the most suitable tillage method for use on the NCP, a field experiment was conducted at Shandong Agricultural University from 2016-2017 using plots that have been farmed using conservation tillage since 2002.

Methods: In this study, Zhengdan 958 summer maize was used as the test material and undisturbed soil and plant samples were obtained under four tillage methods-no-tillage (NT, tillage depth: 0 cm); rotary tillage (RT, tillage depth: 10 cm); conventional tillage (CT, tillage depth: 20 cm); subsoiling (SS, tillage depth: 40 cm)-which were used to determine the AOC and dry matter contents, as well as the yields of two summer maize growing seasons. Each sample was replicated three times and the AOC content was determined via potassium dichromate oxidation colorimetry. Potassium dichromate oxidized organic carbon in organic matter was employed to reduce hexadecent chromium into green trivalent chromium. Colorimetry was then used to determine the amount of reduced trivalent chromium and calculate the organic matter content.

Results: The resulting data were statistically analyzed and the results showed that, compared with CT, the AOC contents with NT and SS increased by 5.65% and 9.73%, respectively, while that with RT decreased by 0.12%. Conventional tillage resulted in the highest mean dry matter weight when the maize reached maturity, which was 19.19%, 9.83%, and 3.38% higher than those achieved using NT, RT, and SS, respectively. No significant difference was found between CT and SS treatments, both of which tended to increase the accumulation of dry matter as well as its contribution of assimilates to grain yield post-anthesis. Compared with CT, the mean yield increased at a rate of 0.18% with SS, while yields declined at rates of 17.17% and 11.15 with NT and RT, respectively. The yield with NT was the lowest, though the harvest indices with NT and SS were higher than those with RT and CT. Overall, SS increased the accumulation of dry matter and its contribution of assimilates to grain yields post-anthesis, as well as the AOC content and yields, making it the ideal tillage method for the NCP.
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http://dx.doi.org/10.7717/peerj.11099DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8000453PMC
March 2021

Effects of CDKN2B-AS1 on cellular proliferation, invasion and AKT3 expression are attenuated by miR-424-5p in a model of ovarian endometriosis.

Reprod Biomed Online 2021 Feb 12. Epub 2021 Feb 12.

Department of Obstetrics and Gynecology, The Second Xiangya Hospital, Central South University, Changsha Hunan 410000, PR China. Electronic address:

Research Question: Endometriosis is a common and complicated gynaecologic disease. Long non-coding RNA CDKN2B-AS1 plays a crucial role in the development and progression of several cancers. Whether CDKN2B-AS1 contributes to endometriosis, however, remains unknown.

Design: Cellular proliferation, invasion and DNA synthesis abilities were assessed by CCK8, transwell and 5-ethynyle-2'-deoxyuridine assays. The expression of epithelial-mesenchymal transition markers and three isoforms of AKT was detected using Western blot. Real-time polymerase chain reaction was used to determine the relative expression levels of CDKN2B-AS1 and candidate miRNAs in ectopic, eutopic endometria and normal endometrial tissues. The relationship between CDKN2B-AS1 and miRNA was determined by luciferase reporter assays.

Results: The relative expression level of CDKN2B-AS1 was up-regulated in eutopic and ectopic endometria. In endometrial stromal cells and Ishikawa cells, CDKN2B-AS1 overexpression promoted cellular proliferation and invasion, and increased the protein expression of vimentin but decreased the expression of E-cadherin. miR-424-5p was confirmed the target of CDKN2B-AS1 through bioinformatics tools and luciferase reporter assays. In addition, the enhanced effect of cellular phenotype of CDKN2B-AS1 overexpression was significantly attenuated by miR-424-5p overexpression. Furthermore, miR-424-5p was able to directly target AKT3 through luciferase reporter assay. Mechanistically, CDKN2B-AS1 acts as a ceRNA by sponging miR-424-5p and targets AKT3.

Conclusions: The cellular mechanism of CDKN2B-AS1 in endometriosis was confirmed; CDKN2B-AS1 may be a potential target for ovarian endometriosis therapy.
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http://dx.doi.org/10.1016/j.rbmo.2021.02.004DOI Listing
February 2021

Responses of microbial communities and their interactions to ibuprofen in a bio-electrochemical system.

J Environ Manage 2021 Apr 2;289:112473. Epub 2021 Apr 2.

Beijing Engineering Research Center of Environmental Material for Water Purification, College of Chemical Engineering, Beijing University of Chemical Technology, Beijing, 100029, China. Electronic address:

Ibuprofen has caused great concerns due to their potential environmental risks. However, their removal efficiency and their effects on microbial interactions in bio-electrochemical system remain unclear. To address these issues, a lab-scale bio-electrochemical reactor integrated with sulfur/iron-mediated autotrophic denitrification (BER-S/IAD) system exposing to 1000 μg L ibuprofen was operated for about two months. Results revealed that the BER-S/IAD system obtained efficient simultaneous denitrification (98.93%) and phosphorus (82.67%) removal, as well as an excellent ibuprofen removal performance (96.98%). Ibuprofen had no significant impacts on the nitrate (NO-N) removal and the ammonia (NH-N) accumulation, but decreased the total nitrogen (TN) and total phosphorus (TP) removal efficiencies. MiSeq sequencing analysis revealed that ibuprofen significantly (P < 0.05) decreased the microbial community diversity and changed their overall structure. Some bacteria related to denitrification and phosphorus removal, such as Pseudomonas and Thiobacillus, decreased significantly (P < 0.05). Moreover, molecular ecological network (MEN) analysis revealed that ibuprofen decreased the network's size and complexity, and enhanced the negative correlations of Proteobacteria and Firmicutes. Besides, ibuprofen decreased the links of some keystone bacteria related to denitrification and phosphorus removal. This research could provide a new dimension for our comprehending of the responses of microbial communities and their interactions to ibuprofen in bio-electrochemical system.
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http://dx.doi.org/10.1016/j.jenvman.2021.112473DOI Listing
April 2021

Characterization of the abnormal cortical effective connectivity in patients with sleep apnea hypopnea syndrome during sleep.

Comput Methods Programs Biomed 2021 Mar 21;204:106060. Epub 2021 Mar 21.

School of Biomedical Engineering, Sun Yat-sen University, China; Key Laboratory of Sensing Technology and Biomedical Instrument of Guangdong Province, Sun Yat-sen University, China. Electronic address:

Background And Objectives: Sleep apnea hypopnea syndrome (SAHS) is a prevalent sleep breathing disorder that can lead to brain damage and is also a risk factor for cognitive impairment and some common diseases. Studies on cortical effective connectivity (EC) during sleep may provide more direct and pathological information and shed new light on brain dysfunction due to SAHS. However, the EC is rarely explored in SAHS patients, especially during different sleep stages.

Methods: To this end, six-channel EEG signals of 43 SAHS patients and 41 healthy participants were recorded by whole-night polysomnography (PSG). The symbolic transfer entropy (STE) was applied to measure the EC between cortical regions in different frequency bands. Posterior-anterior ratio (PA) was employed to evaluate the posterior-to-anterior pattern of information flow based on overall cortical EC. The statistical characteristics of the STE and PA and of the intra-individual normalized parameters (STE* and PA*) were served as different feature sets for classifying the severity of SAHS.

Results: Although the patterns of STE across electrodes were similar, significant differences were found between the patient and the control groups. The variation trends across stages in the PA were also different in multiple frequency bands between groups. Important features extracted from the STE* and PA* were distributed in multiple rhythms, mainly in δ, α, and γ. The PA* feature set gave the best results, with accuracies of 98.8% and 83.3% for SAHS diagnosis (binary) and severity classification (four-way).

Conclusions: These results suggest that modifications in cortical EC were existed in SAHS patients during sleep, which may help characterize cortical abnormality in patients.
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http://dx.doi.org/10.1016/j.cmpb.2021.106060DOI Listing
March 2021

Oral delivery of staphylococcal nuclease ameliorates DSS induced ulcerative colitis in mice via degrading intestinal neutrophil extracellular traps.

Ecotoxicol Environ Saf 2021 Jun 31;215:112161. Epub 2021 Mar 31.

College of Life Science and Technology, China Pharmaceutical University, Nanjing, China. Electronic address:

Recent studies have revealed that neutrophil extracellular traps (NETs) may contribute directly to the initiation of ulcerative colitis (UC), a typical inflammatory bowel disease (IBD) characterized by mucosal damage. Staphylococcal nuclease (SNase), a nonspecific phosphodiesterase, has a strong ability to degrade DNA. Here we investigate whether intestinal NET degradation with an oral preparation of SNase can ameliorate dextran sulfate sodium (DSS)-induced UC in mice. SNase encapsulated with calcium alginate (ALG-SNase) was formulated using crosslinking technology with sodium alginate and calcium chloride. ALG-SNase were orally administered to DSS-induced UC mice, and their therapeutic efficacy was evaluated. The expression of inflammatory cytokines and biomarkers of NETs was also assessed, as well as the intestinal permeability in mice. The results showed that ALG-SNase nanoparticles were successfully prepared and delivered to the colon of UC mice. In addition, oral administration of ALG-SNase nanoparticles decreased NET levels in the colon and effectively alleviated the clinical colitis index and tissue inflammation in UC mice. Moreover, the SNase nanoparticles reduced intestinal permeability and regulated the expression of proinflammatory cytokines. Furthermore, the markers of NETs were strongly correlated with the expression levels of tight junction proteins in colon tissue. In conclusion, our data showed that oral administration of ALG-SNase can effectively ameliorate colitis in UC mice via NET degradation and suggested SNase as a candidate therapy for the treatment of UC.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112161DOI Listing
June 2021

Ag/Ultrathin-Layered Double Hydroxide Nanosheets Induced by a Self-Redox Strategy for Highly Selective CO Reduction.

ACS Appl Mater Interfaces 2021 Apr 1. Epub 2021 Apr 1.

State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, People's Republic of China.

The carbon-neutral photocatalytic CO reduction reaction (CORR) enables the conversion of CO into hydrocarbon fuels or value-added chemicals under mild conditions. Achieving high selectivity for the desired products of the CORR remains challenging. Herein, a self-redox strategy is developed to construct strong interfacial bonds between Ag nanoparticles and an ultrathin CoAl-layered double hydroxide (U-LDH) nanosheet support, where the surface hydroxyl groups associated with oxygen vacancies of U-LDH play a critical role in the formation of the interface structure. The supported Ag@U-LDH acts as a highly efficient catalyst for CO reduction, resulting in a high CO evolution rate of 757 μmol g h and a CO selectivity of 94.5% under light irradiation. Such a high catalytic selectivity may represent a new record among current photocatalytic CORR to CO systems. The Ag-O-Co interface bonding is confirmed by Fourier-transform infrared (FTIR) spectroscopy, Raman spectroscopy, X-ray photoelectron spectroscopy, and FTIR CO adsorption studies. The in situ FTIR measurements indicate that the formation of the *COOH intermediate is accelerated and the mass transfer is improved during the CORR. Density functional theory calculations show that the Ag-O-Co interface reduces the formation energy of the *COOH intermediate and accumulates localized charge. Experimental and theoretical analysis collectively demonstrates that the strong interface bonding between Ag and U-LDH activates the interface structure as catalytically CORR active sites, effectively optimizing the binding energies with reacted intermediates and facilitating the CORR performance.
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http://dx.doi.org/10.1021/acsami.1c02737DOI Listing
April 2021

Role of DNA methylation-related chromatin remodeling in aryl hydrocarbon receptor-dependent regulation of T-2 toxin highly inducible Cytochrome P450 1A4 gene.

FASEB J 2021 May;35(5):e21469

Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, P.R. China.

Mycotoxins are toxic secondary metabolites produced by food-contaminating fungi, which lead to global epigenetic changes and cause toxicity to both farm animals and humans. However, whether mycotoxins induce gene-specific epigenetic alterations associated with inducible downstream gene expression is unclear as are the underlying regulatory mechanisms. Here, we found that T-2 toxin and its deacetylated metabolites but not deoxynivalenol (DON) or other representative mycotoxins highly induced the expression of cytochrome P450 1A4 (CYP1A4) in both Leghorn male hepatoma (LMH) cells and chicken primary hepatocytes, and this effect was related to the regulation of both aryl hydrocarbon receptor (AhR) and DNA methylation. We used methylation-sensitive restriction enzyme digestion-qPCR (MSRE-qPCR) and chromatin immunoprecipitation (ChIP) assays and found that the binding of DNA methyltransferase 1 (DNMT1) and histone deacetylase 2 (HDAC2) to highly methylated CpG island 3-2 at the enhancer of CYP1A4 was accompanied by the recruitment of the repressive histone modification marker H3K27me3, inducing a silent state. In turn, T-2 toxin stimulation enriched the binding of AhR to demethylated CpG island 3-2, which facilitated p300 and H3K9ac recruitment and ultimately generated an activated chromatin structure at the enhancer by increasing the active histone modification markers, including H3K4me3, H3K27ac, and H3K14ac. Interestingly, T-2 toxin-induced AhR activation also facilitated RNA polymerase II binding to CpG island 2, which may form a transcriptionally active chromatin structure at the promoter and ultimately transactivate CYP1A4. Our findings provide novel insights into the epigenetic regulation of T-2 toxin-induced gene expression.
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http://dx.doi.org/10.1096/fj.202002570RRDOI Listing
May 2021

Down-regulation of HLA-DRs and HLA-DPs reflects the deficiency of antigen-presenting cells in endometrium from infertile women with and without ovarian endometriosis.

Hum Fertil (Camb) 2021 Mar 31:1-12. Epub 2021 Mar 31.

Department of Obstetrics and Gynecology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China.

This study aimed to explore common molecular changes in the infertile endometrium from women with and without endometriosis (EM). By analyzing the dataset GSE120103 from Gene Expression Omnibus, a total of 3252 shared differentially expressed genes were identified between ovarian EM in infertile vs. fertile endometrium and EM-free infertile vs. fertile endometrium. In addition, the gene annotation and pathway analysis of the shared differentially expressed genes with the same expression trend indicated that the pathway 'MHC class II antigen presentation' and five candidate genes: HLA-DRA, HLA-DRB1, HLA-DRB3, HLA-DPA1, HLA-DPB1 were both down-regulated in infertile endometrium with or without EM. Logistic regression showed that HLA-DRA might be an independent predictor of the infertile status of the endometrium. Single sample gene set enrichment analysis (ssGSEA) showed that some classic antigen-presenting cells: macrophages type 1, macrophages type 2, and mature dendritic cells were significantly down-regulated in infertile endometrium with or without EM, whose enrichment correlated positively with the expression of candidate HLA molecules. Hence, the down-regulation of HLA-DRs and HLA-DPs reflecting the deficiency of antigen-presenting cells in endometrium might serve as a common biomarker for diagnosing endometrium-associated infertility in women with and without endometriosis.
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http://dx.doi.org/10.1080/14647273.2021.1902576DOI Listing
March 2021

Relationships between soil respiration and hyperspectral vegetation indexes and crop characteristics under different warming and straw application modes.

Environ Sci Pollut Res Int 2021 Mar 26. Epub 2021 Mar 26.

School of Applied Meteorology, Nanjing University of Information Science and Technology, Nanjing, 210044, China.

Examining the relationship between seasonal variations in soil respiration and abiotic factors and vegetation indexes is crucial for modeling soil respiration using upscaled remote sensing satellite data. A field experiment including control (CK), warming (WA), straw application (SA), and warming and straw application (WASA) treatments was performed in a winter wheat-soybean rotation cropland on the north shore of the lower reaches of the Yangtze River. Soil respiration, abiotic factors, crop hyperspectral vegetation indexes, leaf area index (LAI), and chlorophyll content (represented as the SPAD value) were measured during the 2018-2020 rotation growing seasons. The results indicated that the mean annual soil respiration was 2.27 ± 0.04, 3.08 ± 0.06, 3.64 ± 0.08, and 3.95 ± 0.20 μmol m s in the CK, WA, SA, and WASA plots, respectively, during the 2-year experimental period. Soil respiration was significantly (P < 0.05) correlated with soil temperature, soil moisture, hyperspectral vegetation indexes, LAI, and SPAD value in all plots. Models that included temperature, moisture, hyperspectral vegetation indexes, LAI, and SPAD value explained 50.5-74.7% of the seasonal variation in soil respiration in the CK, WA, SA, and WASA plots during the 2-year experimental period. A model including the seasonal mean NDVI, DVI, EVI, PRI, and LAI explained 72.4% of the interseasonal and intertreatment variations in seasonal mean soil respiration in the different plots across the four different crop-growing seasons. Our study indicated the potential applicability of hyperspectral vegetation indexes, LAI, and SPAD value to the estimation of soil respiration at a regional scale.
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http://dx.doi.org/10.1007/s11356-021-13612-3DOI Listing
March 2021

A novel mutation in NF1 gene of patient with Neurofibromatosis type 1: A case report and functional study.

Mol Genet Genomic Med 2021 Mar 25:e1643. Epub 2021 Mar 25.

Department of Pediatrics, Ruijin Hospital Affiliated to Shanghai Jiao Tong University, Shanghai, China.

Background: Neurofibromatosis type 1 is an autosomal dominant inherited disease and caused by NF1 gene mutation. Its clinical manifestations include multiple cafe´-au lait (CAL) spots, skinfold freckling, neurofibroma, bone dysplasia, learning disabilities, and an increased risk of malignancy.

Methods And Results: Here, we reported a Chinese patient bearing with a novel NF1 mutation (c.2064delGGATGCAGCGG/p.Gly672AsnfsTer24) and complaining mainly about bone phenotype. Functional studies found that this novel mutation caused the damage of NF1 mRNA and protein levels, and lost the inhibition on Ras/Erk signaling.

Conclusion: A novel mutation in NF1 gene was identified and in vitro functional studies were performed, which provided a potential molecular mechanism to explain the bone maldevelopment of patients with neurofibromatosis type 1.
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http://dx.doi.org/10.1002/mgg3.1643DOI Listing
March 2021

Compensatory combination of mTOR and TrxR inhibitors to cause oxidative stress and regression of tumors.

Theranostics 2021 25;11(9):4335-4350. Epub 2021 Feb 25.

The First Affiliated Hospital of Wenzhou Medical University, Wenzhou Medical University, Wenzhou 325035, China.

Cancer is a leading cause of death worldwide. Extensive research over decades has led to the development of therapies that inhibit oncogenic signaling pathways. The mammalian target of rapamycin (mTOR) signaling pathway plays an important role in the development of many cancers. Several mTOR inhibitors are approved for the treatment of cancers. However, the anticancer efficacies of mTOR inhibitor monotherapy are still limited. Western blot was used to detect the expression of indicated molecules. Thioredoxin reductase (TrxR) activity in cells was determined by the endpoint insulin reduction assay. Immunofluorescence staining was used to analyze precise location and expression of target proteins. Nude mice were used for xenograft tumor models. We identified a synergistic lethal interaction of mTOR and TrxR inhibitors and elucidated the underlying molecular mechanisms of this synergism. We demonstrated that mTOR and TrxR inhibitors cooperated to induce cell death by triggering oxidative stress, which led to activation of autophagy, endoplasmic reticulum (ER) stress and c-Jun N-terminal Kinase (JNK) signaling pathway in cancer cells. Remarkably, we found that auranofin (AF) combined with everolimus significantly suppressed tumor growth in HCT116 and SGC-7901 xenograft models with no significant signs of toxicity. Our findings identify a promising therapeutic combination for cancer and has important implications for developing mTOR inhibitor-based combination treatments.
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http://dx.doi.org/10.7150/thno.52077DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7977446PMC
February 2021

miR-182-5p and miR-96-5p Target and Mediate the Negative Feedback Regulatory Loop between PIAS1 and STAT3 in Endometrial Cancer.

DNA Cell Biol 2021 Apr 22;40(4):618-628. Epub 2021 Mar 22.

Department of Obstetrics and Gynecology, The Second Xiangya Hospital, Central South University, Changsha, China.

The expressions and roles of protein inhibitor of activated STAT (PIAS) proteins, a group of proteins with STAT inhibition and SUMOylation E3 ligase activity, are rarely revealed in endometrial cancer (EC). In this study, we analyzed the expressions of PIASs and their relationships with clinical features by mining online data through web servers, including UALCAN and Gene Expression Profiling Interactive Analysis (GEPIA) in EC. The expressions of PIASs in EC tissues were further validated by immunohistochemistry (IHC). The online analyses revealed only PIAS1 was consistently downregulated both at mRNA and protein level in EC, which was validated by the IHC. Subsequently, the mechanism of PIAS1 downregulation was explored with online tools like UALCAN, cBioPortal, LinkedOmics, and the Encyclopedia of RNA Interactomes (ENCORI). The results indicated that the mutation rate of PIAS1 was extremely low and not associated with PIAS1 expression. The promoter methylation level of PIAS1 was comparable between normal and EC tissues. miR-182-5p and miR-96-5p with negative association with PIAS1 in EC were predicted to target PIAS1. Dual luciferase reporter assay confirmed miR-182-5p and miR-96-5p could target PIAS1 in EC. MiR-182-5p and miR-96-5p inhibitors could upregulate PIAS1 in EC cells. Moreover, ectopic PIAS1 expression and STAT3 inhibitor treatment significantly inhibited STAT3's activity and the levels of miR-182-5p and miR-96-5p in EC cells. Collectively, our findings revealed PIAS1 was downregulated in EC, which was caused by upregulation of miR-182-5p and miR-96-5p, and PIAS1 downregulation further activated STAT3 and increased the expression of miR-182-5p and miR-96-5p, confirming miR-182-5p and miR-96-5p mediated the negative feedback regulatory loop between PIAS1 and STAT3 in EC.
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http://dx.doi.org/10.1089/dna.2020.6379DOI Listing
April 2021

Cloning, analysis, and anti-duck Tembusu virus innate immune response of Cherry Valley duck tripartite motif-containing 32.

Poult Sci 2021 Feb 15;100(5):101048. Epub 2021 Feb 15.

Sino-German Cooperative Research Centre for Zoonosis of Animal Origin of Shandong Province, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an City 271018, Shandong Province, China; Collaborative Innovation Center for the Origin and Control of Emerging Infectious Diseases, College of Basic Medical Sciences, Shandong First Medical University, Tai'an City 271000, Shandong Province, China. Electronic address:

Tripartite motif-containing 32 (TRIM32) is an E3 ubiquitin ligase with multiple functions. In this study, we amplified TRIM32 gene from the Cherry Valley duck, and its cDNA sequence contained an open reading frame of 1,950 bp that encodes 649 amino acids. Duck TRIM32 (duTRIM32) mRNA was expressed in all tissues tested. A series of immune-related genes that were induced by viral infection, including interferon alfa, IL-1β, retinoic acid-inducible gene-I, Mx, and OAS, were regulated by duTRIM32 expression. DuTRIM32 overexpression inhibits duck Tembusu virus (DTMUV) replication in the early stages of viral infection. Knockdown of duTRIM32 expression by siRNA reduced the ability of duck embryo fibroblast cells to mount a type Ⅰ interferon response to DTMUV. Therefore, our results suggest that the duTRIM32-mediated signal pathway plays an essential role in DTMUV infection-induced innate immune response.
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http://dx.doi.org/10.1016/j.psj.2021.101048DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8005831PMC
February 2021

The ABCA1-efferocytosis axis: A new strategy to protect against atherosclerosis.

Clin Chim Acta 2021 Mar 16;518:1-8. Epub 2021 Mar 16.

Cancer Institute, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao Cancer Institute, Qingdao, Shandong 266071, China; School of Life Sciences, Tsinghua University, Beijing 100084, China. Electronic address:

Atherosclerosis, a disease process characterized by lipid accumulation and inflammation, is the main cause of coronary heart disease (CHD) and myocardial infarction (MI). Efferocytosis involves the clearance of apoptotic cells by phagocytes. Successful engulfment triggers the release of anti-inflammatory cytokines to suppress atherosclerosis. ABCA1 is a key mediator of cholesterol efflux to apoA-I for the generation of HDL-C in reverse cholesterol transport (RCT). Intriguingly, ABCA1 promotes not only cholesterol efflux but also efferocytosis. ABCA1 promotes efferocytosis by regulating the release of "find-me" ligands, including LPC, and the exposure, release, and expression of "eat-me" ligands, including PtdSer, ANXA1, ANXA5, MEGF10, and GULP1. ABCA1 has a pathway similar to TG2, which is an "eat-me" ligand. ABCA1 has the highest known homology to ABCA7, which controls efferocytosis as the engulfment and processing ligand. In addition, ABCA1 can form several regulatory feedback axes with ANXA1, MEGF10, GULP1, TNFα, and IL-6. Therefore, ABCA1 is the central factor that links cholesterol efflux and apoptotic cell clearance. Several drugs have been studied or approved for apoptotic cell clearance, such as CD47 antibody and PD1-/PD-L1 antibody. In this article, we review the role and mechanism of action of ABCA1 in efferocytosis and focus on new insights into the ABCA1-efferocytosis axis and its potential as a novel therapeutic target in atherosclerosis.
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http://dx.doi.org/10.1016/j.cca.2021.02.025DOI Listing
March 2021

Large-scale functional network connectivity mediate the associations of gut microbiota with sleep quality and executive functions.

Hum Brain Mapp 2021 Mar 19. Epub 2021 Mar 19.

Department of Radiology, The First Affiliated Hospital of Anhui Medical University, Hefei, China.

Network neuroscience has broadly conceptualized the functions of the brain as complex communication within and between large-scale neural networks. Nevertheless, whether and how the gut microbiota influence functional network connectivity that in turn impact human behaviors has yet to be determined. We collected fecal samples from 157 healthy young adults and used 16S sequencing to assess gut microbial diversity and enterotypes. Large-scale inter- and intranetwork functional connectivity was measured using a combination of resting-state functional MRI data and independent component analysis. Sleep quality and core executive functions were also evaluated. Then, we tested for potential associations between gut microbiota, functional network connectivity and behaviors. We found significant associations of gut microbial diversity with internetwork functional connectivity between the executive control, default mode and sensorimotor systems, and intranetwork connectivity of the executive control system. Moreover, some internetwork functional connectivity mediated the relations of microbial diversity with sleep quality, working memory, and attention. In addition, there was a significant effect of enterotypes on intranetwork connectivity of the executive control system, which could mediate the link between enterotypes and executive function. Our findings not only may expand existing biological knowledge of the gut microbiota-brain-behavior relationships from the perspective of large-scale functional network organization, but also may ultimately inform a translational conceptualization of how to improve sleep quality and executive functions through the regulation of gut microbiota.
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http://dx.doi.org/10.1002/hbm.25419DOI Listing
March 2021

A Three-Dimensional Branched TiO Photoanode with an Ultrathin AlO Passivation Layer and a NiOOH Cocatalyst toward Photoelectrochemical Water Oxidation.

ACS Appl Mater Interfaces 2021 Mar 16;13(11):13301-13310. Epub 2021 Mar 16.

School of Petrochemical Engineering, Changzhou University, Changzhou, Jiangsu 213164, China.

Photoelectrochemical (PEC) water splitting provides an alternative strategy for clean and renewable hydrogen production; however, the practical application is severely limited by the low solar conversion. Herein, a novel and simple strategy has been developed to construct a 3D branched TiO photoanode with an ultrathin AlO passivation layer and NiOOH cocatalyst. The structure and properties of the as-obtained photoanodes are explored by X-ray diffraction, Mott-Schottky, electrochemical impedance spectroscopy, and open circuit voltage measurements. The as-obtained B-TiO/AlO/NiOOH ternary heterojunction with a high-quality contact interface exhibits improved light absorption ability, an enhanced photocurrent density of 1.42 mA/cm at 1.23 V, high conversion efficiency (0.44% at 0.80 V), and excellent stability compared to pristine TiO and alone-AlO or NiOOH decorated TiO photoanodes. Therefore, this work could offer a new approach to designing and fabricating high-quality contact interfaces between photoelectrodes and various cocatalysts.
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http://dx.doi.org/10.1021/acsami.1c00948DOI Listing
March 2021

In situ sulfidation of porous sponge-like CuO/SiWCo into CuS/SiWCo as stabilized and efficient counter electrode for quantum dot-sensitized solar cells.

Dalton Trans 2021 Apr 15;50(13):4519-4526. Epub 2021 Mar 15.

Key Laboratory of Polyoxometalates Science of Ministry of Education, College of Chemistry, Northeast Normal University, Changchun 130024, P. R. China.

Quantum dot-sensitized solar cells (QDSSCs), which are cost-effective, facilely prepared and environmentally friendly, are regarded as a promising third-generation photovoltaic technology, although the conversion efficiency of QDSSCs is still far lower than the theoretical efficiency (44%). Furthermore, the traditional CuS/brass counter electrode (CE) is susceptible to continuous corrosion from the polysulfide electrolyte, reducing the stability and electrocatalytic activity of the CE, thereby affecting cell performance. To enhance the stability of the CuS CE and make full use of its excellent catalytic properties, we used eco-friendly polyoxometalates (KSiWOCo(ii) (HO), SiWCo) and porous sponge-like CuO as precursors to prepare a novel SiWCo-doped high-efficiency CuS/SiWCo CE on FTO by adopting the method of in situ sulfidation in the electrolyte. Electrochemical analysis revealed that the novel CuS/2%-SiWCo (CuS-2) CE exhibits extremely high stability and electrocatalytic performance compared with other electrodes. Meanwhile, adding an appropriate amount of SiWCo can inhibit charge recombination and improve cell performance. The CdS/CdSe co-sensitized QDSSCs assembled with the novel CuS-2 CE obtained a power conversion efficiency (PCE) of 5.94%, which was 20% more efficient than QDSSCs based on CuS/brass CE (4.96%). The method reported here puts forth a new direction for the preparation of efficient CuS counter electrodes.
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http://dx.doi.org/10.1039/d1dt00247cDOI Listing
April 2021

A Survival-Related Competitive Endogenous RNA Network of Prognostic lncRNAs, miRNAs, and mRNAs in Wilms Tumor.

Front Oncol 2021 26;11:608433. Epub 2021 Feb 26.

Department of Pediatric Surgery, The Second Hospital Affiliated to Harbin Medical University, Harbin, China.

Wilms tumor (WT) commonly occurs in infants and children. We evaluated clinical factors and the expression of multiple RNAs in WT samples in the TARGET database. Eight long non-coding RNAs (lncRNAs; AC079310.1, MYCNOS, LINC00271, AL445228.3, Z84485.1, AC091180.5, AP002518.2, and AC007879.3), two microRNAs (miRNAs; hsa-mir-152 andhsa-mir-181a), and nine messenger RNAs (mRNAs; TCTEX1D4, RNF133, VRK1, CCNE1, HEY1, C10orf71, SPRY1, SPAG11A, and MAGEB18) were screened from differentially expressed RNAs and used to construct predictive survival models. These models showed good prognostic ability and were highly correlated with tumor stage and histological classification. Additionally, survival-related ceRNA network was constructed using 35 RNAs (15 lncRNAs, eight miRNAs, and 12 mRNAs). KEGG pathway analysis suggested the "Wnt signaling pathway" and "Cellular senescence" as the main pathways. In conclusion, we established a multinomial predictive survival model and a survival-related ceRNA network, which provide new potential biomarkers that may improve the prognosis and treatment of WT patients.
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http://dx.doi.org/10.3389/fonc.2021.608433DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7953909PMC
February 2021

Proteomics reveals the function reverse of MPSSS-treated prostate cancer-associated fibroblasts to suppress PC-3 cell viability via the FoxO pathway.

Cancer Med 2021 Apr 11;10(7):2509-2522. Epub 2021 Mar 11.

Key Laboratory of Protein and Peptide Pharmaceuticals & Laboratory of Proteomics, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.

Prostate cancer-associated fibroblasts (prostate CAFs) are essential components of the tumor microenvironment and can promote tumor progression through their immunosuppressive functions. MPSSS, a novel polysaccharide purified from Lentinus edodes, has been reported to have anti-tumor activity. MPSSS could also inhibit the immunosuppressive function of prostate CAFs, which has been demonstrated through that the secretome of MPSSS-treated prostate CAFs could inhibit the proliferation of T cells. However, how the secretome of MPSSS-treated prostate CAFs influence prostate cancer progression is still unclear. Interestingly, we found that the low molecular weight (3-100kD) secretome of prostate CAFs (lmwCAFS) could promote the growth of PC-3 cells, while that of MPSSS-treated prostate CAFs (MT-lmwCAFS) could inhibit their growth. We carried out comparative secretomic analysis of lmwCAFS and MT-lmwCAFS to identify functional molecules that inhibit the growth of PC-3 cells, and proteomic analysis of lmwCAFS-treated PC-3 cells and MT-lmwCAFS-treated PC-3 cells to investigate the underlying molecular mechanism. These analyses suggest that TGF-β3 from MT-lmwCAFS may inhibit the growth of PC-3 cells. The validated experiments revealed that TGF-β3 from MT-lmwCAFS activated p21 expression in PC-3 cells by regulating the FoxO pathway thereby inducing G0/G1 cell cycle arrest of PC-3 cells. Overall, our data demonstrated that MPSSS reversed the ability of prostate CAFs to suppress the cell viability of PC-3 cells, which might provide a potential therapeutic strategy to prevent prostate cancer progression.
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http://dx.doi.org/10.1002/cam4.3825DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982613PMC
April 2021

TRIM32 inhibits the proliferation and migration of pulmonary artery smooth muscle cells through the inactivation of PI3K/Akt pathway in pulmonary arterial hypertension.

J Bioenerg Biomembr 2021 Mar 10. Epub 2021 Mar 10.

Department of Cardiovascular Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, No. 277 West Yanta Road, Xi'an, Shaanxi, 710061, China.

Pulmonary arterial hypertension (PAH) is a progressive and fetal cardiovascular disease. Tripartite motif 32 (TRIM32) is a member of TRIM family that has been found to be involved in cardiovascular disease. However, the role of TRIM32 in PAH remains unclear. Here we investigated the effects of TRIM32 on hypoxia-induced pulmonary artery smooth muscle cells (PASMCs) in vitro. Our results showed that TRIM32 protein level in the plasma samples from PAH patients was decreased as compared with healthy volunteers. Exposure to hypoxia condition caused a significant decrease in TRIM32 expression in PASMCs. Overexpression of TRIM32 inhibited hypoxia-induced proliferation and migration of PASMCs. TRIM32 overexpression elevated the increased apoptotic rate and caspase-3 activity in hypoxia-induced PASMCs. Moreover, overexpression of TRIM32 reversed hypoxia-induced down-regulation of myocardin, SM 22 and calponin, as well as up-regulation of osteopontin (OPN). Whereas, TRIM32 knockdown shwed the opposite effect. Furthermore, overexpression of TRIM32 inhibited hypoxia-induced activation of PI3K/Akt with decreased phosphorylated level of PI3K and Akt. Additionally, activation of PI3K/Akt by IGF-1 treatment reversed the effects of TRIM32 on hypoxia-induced PASMCs. In conclusion, these findings indicated that TRIM32 was involved in the development of PAH through regulating the proliferation, migration, apoptosis and dedifferentiation of PASMCs, which might be mediated by the PI3K/Akt signaling pathway. Thus, TRIM32 might be a potential target for PAH treatment.
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http://dx.doi.org/10.1007/s10863-021-09880-wDOI Listing
March 2021

Loosening Neuro-Optic Structures Dosimetric Constraints Provides High 5-Year Local Recurrence-Free Survival With Acceptable Toxicity in T4 Nasopharyngeal Carcinoma Patients Treated With Intensity-Modulated Radiotherapy.

Front Oncol 2021 22;11:598320. Epub 2021 Feb 22.

Department of Radiation Oncology, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.

Objective: Whether the original dosimetric constraints of neuro-optic structures (NOS) are appropriate for patients with nasopharyngeal carcinoma (NPC) undergoing intensity-modulated radiotherapy (IMRT) remains controversial. The present study compared the survival rates and radiation-induced optic neuropathy (RION) occurrence between T4 NPC patients whose NOS were irradiated with a near maximum dose received by 2% of the volume (D2%) >55 Gy and ≤55 Gy. Moreover, the NOS dosimetric parameters and their correlation with RION occurrence were also evaluated.

Methods: In this retrospective study, 256 T4 NPC patients treated with IMRT between May 2009 and December 2013 were included. Patient characteristics, survival rates, dosimetric parameters, and RION incidence were compared between the D2% ≤55 Gy and D2% >55 Gy groups.

Results: The median follow-up durations were 87 and 83 months for patients in the D2% >55 Gy and D2% ≤55 Gy groups, respectively. The 5-year local recurrence-free survival rates were 92.0 and 84.0% in the D2% >55 Gy and D2% ≤55 Gy groups (P = 0.043), respectively. There was no significant difference in the 5-year overall survival (OS) between both groups (D2% >55 Gy, 81.6%; D2% ≤55 Gy, 79.4%; P = 0.586). No patients developed severe RION (Grades 3-5), and there was no significant difference (P = 0.958) in the incidence of RION between the two groups. The maximum dose of NOS significantly affected the RION incidence, with a cutoff point of 70.77 Gy.

Conclusion: Appropriately loosening NOS dosimetric constraints in order to ensure a more sufficient dose to the target volume can provide a better 5-year local recurrence-free survival and acceptable neuro-optic toxicity in T4 NPC patients undergoing IMRT.
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http://dx.doi.org/10.3389/fonc.2021.598320DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7937960PMC
February 2021

Changes in plasma HDL and its subcomponents HDL2b and HDL3 regulate inflammatory response by modulating SOCS1 signaling to affect severity degree and prognosis of sepsis.

Infect Genet Evol 2021 Mar 5;91:104804. Epub 2021 Mar 5.

Department of Intensive Care Unit, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, Guangdong 510180, China.

Objectives: To explore if SOCS1 is regulated by plasma HDL and its subcomponents HDL2b and HDL3 to affect inflammatory reaction then to influence the severity degree and prognosis of sepsis.

Methods: One hundred sepsis patients in ICU and 85 normal control persons from October 2018 to October 2019 in our hospital were enrolled. Adult male C57BL/6 mice were used to establish sepsis model by CLP method. HDL, CRP, and WBC count of human were measured using an auto-analyzer. Plasma HDL, IL-1β, and TNF-α proteins levels of mice were measured with ELISA. Microfluidic chip was used for plasma HDL2b and HDL3 detections. SOCS1 in liver and spleen of mice were measured by qRT-PCR. The relationship between plasma HDL//HDL2b and inflammatory indices/SOCS1 in liver/spleen was analyzed with spearman correlation coefficient method. The sepsis patients/mice were divided into non-survival and survival groups. The sepsis patients were divided into severe and mild sepsis patients based on the SOFA score or divided into high and low score groups according to the APACHE II score. The sepsis mice were divided into high and low score group based on the modified sepsis severity score criterion.

Results: Plasma HDL and HDL2b levels were significantly declined (P < 0.01), while HDL3 was normal in both sepsis patients and mice (P > 0.05). Plasma HDL and HDL2b were negatively associated with the serum CRP concentration and positively correlated with the prognosis and severity in sepsis patients (P < 0.05). Moreover, the downregulated plasma HDL but not HDL2b was negatively related to increased SOCS1 mRNA levels in liver and spleen of mice, which were positively connected with TNF-α and IL-1β protein levels (P < 0.05).

Conclusions: Plasma HDL is downregulated in sepsis, which may facilitate inflammatory reaction then activate the SOCS1 signaling to regulate the severity and affect prognosis of sepsis. The decline of plasma HDL2b content could aggravate the severity and poor prognosis of sepsis through facilitating inflammatory reaction. The plasma HDL3 is not involved in sepsis. The more and further explorations may be needed.
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http://dx.doi.org/10.1016/j.meegid.2021.104804DOI Listing
March 2021

Changes of serum immunoglobulin level in healthy pregnant women and establishment of its reference interval.

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2021 Jan;46(1):53-59

Department of Laboratory Medicine, Second Xiangya Hospital, Central South University, Changsha 410011.

Objectives: Pregnant women in a special physiological period, the body's blood indicators will change to a certain extent. This study aims to explore the changes of serum immunoglobulin levels in healthy pregnant women and establish its reference interval (RI).

Methods: A total of 369 healthy pregnant women, who underwent pregnancy examination in the Department of Obstetrics, Second Xiangya Hospital of Central South University from August 2019 to October 2019, were enrolled for this study. They were divided into an early pregnancy group, a middle pregnancy group, and a late pregnancy group according to the pregnancy period, and 123 healthy non-pregnant women were selected as the controls. The levels of immunoglobulin G (IgG), immunoglobulin M (IgM), and immunoglobulin A (IgA) were determined by immune transmission turbidities. The level of immunoglobulin E (IgE) was determined by electrochemiluminescence. The differences in immunoglobulin levels between pregnant women and non-pregnant women and among different gestational periods were analyzed, and the RI of serum immunoglobulin level during pregnancy was established.

Results: Compared to the non-pregnant women, the levels of serum IgG, IgM, IgA, and IgE in pregnant women were significantly decreased (all <0.01), with 51.81% for IgG, 43.84% for IgM, 55.80% for IgA, and 49.80% for IgE. Except that the IgG level of late pregnancy group was significantly lower than that of early pregnancy group (<0.05), there were no significant differences in the IgG, IgM, IgA, and IgE levels among the other groups (all >0.05). The RIs of serum IgG in early pregnancy, middle pregnancy, and late pregnancy were 6.02-7.70 g/L, 5.18-6.85 g/L, and 4.58-5.72 g/L, respectively, while the RIs of serum IgM, IgA, and IgE were 0.71-0.93 g/L, 0.90-1.09 g/L, and 68.30-107.69 ng/mL, respectively in pregnant women.

Conclusions: The levels of immunoglobulin in pregnant women are decreased significantly. The establishment of RIs of IgG, IgM, IgA and IgE in healthy pregnant women could provide scientific basis for clinical decision-making.
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http://dx.doi.org/10.11817/j.issn.1672-7347.2021.200468DOI Listing
January 2021

Use of Antiretroviral Therapy for a US Medicaid Enrolled Pediatric Cohort with HIV.

AIDS Behav 2021 Mar 4. Epub 2021 Mar 4.

Department of Health Services, Policy & Practice, Brown University School of Public Health, 121 South Main Street, Providence, Rhode Island, 02903, USA.

Appropriate antiretroviral therapy use in children with Human Immunodeficiency Virus (HIV) is essential for optimizing clinical outcomes and preventing HIV transmission. To describe and determine correlates of HIV antiretroviral therapy (ART) persistence and implementation for children and adolescents in the United States. We studied Medicaid enrollees (ages 2-19 years) with HIV in 14 states in 2011 and 2012. We defined non-persistence as a discontinuation of an ART regimen for at least 90 days, and calculated implementation as the proportion of days on ART while persistent. We used Cox proportional regression and logistic regression to determine characteristics associated with ART non-persistence and poor (< 90%) implementation, respectively. Among those with ≥ 1 year of observation (n = 8679), 55.7% never received ART. For ART recipients (n = 3849), 34.9% discontinued ART. Correlates of ART non-persistence included older age (e.g., 15-19 vs. 2-5 years [adjusted hazard ratio (aHR) 2.9, 95% CI 2.1-4.0]; females vs. males (aHR 1.2; 1.1-1.3); mental health conditions (aHR 1.3; 1.1-1.5), drug/alcohol abuse (aHR 1.2; 1.0-1.5) and HIV-related conditions (aHR 1.2; 1.0-1.4). Those with an outpatient visit were less likely to discontinue an ART (aHR 0.32; 0.28-0.36). During persistent episodes, 42.3% had poor ART implementation. Correlates of poor implementation included females vs. males (aOR 1.2; 95% CI 1.0-1.3), Black vs. White race (aOR 1.3; 95% CI 1.1-1.7) and Hispanic/Latino vs. White (aOR 1.3; 1.0-1.8). Among Medicaid youth with HIV, there were low rates of ART exposure, and ART discontinuation was common. Correlates of persistence and implementation differed, suggesting a need for varying clinical interventions to improve connection to care and ensuring ongoing engagement with ART use.
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http://dx.doi.org/10.1007/s10461-021-03208-wDOI Listing
March 2021

High-Efficiency All-Inorganic Perovskite Solar Cells Tailored by Scalable Rutile TiO Nanorod Arrays with Excellent Stability.

ACS Appl Mater Interfaces 2021 Mar 4;13(10):12091-12098. Epub 2021 Mar 4.

Institute of New Energy Technology, College of Information Science and Technology, Jinan University, Guangzhou 510632, PR China.

Tailored optimization of perovskite solar cells (PSCs) is a persistent objective to achieve the ultimate commercialization purpose, in which the electron/hole transport layer with thickness on the nanometer scale is generally required to maximize the charge collection and minimize the series resistance. Therefore, precise control on the fabrication technology of the charge transport layer is important. Herein, one-dimensional (1D) rutile TiO nanorod arrays with a thickness of 1.8 μm have been fabricated and employed as a potential electron extraction layer for high-efficiency all-inorganic CsPbBr PSCs for the first time. Arising from the sufficient carrier mobility, excellent conductivity, and superior charge extraction ability by means of regulating the donor concentration with nitrogen atoms, a champion efficiency of 8.50% has been achieved with excellent long-term stability after 50 days storage in air conditions, which is comparable to that of the 200 nm-thick TiO layer tailored device. The primary results demonstrate that the TiO layer with micrometer scale thickness is also feasible to effectively collect the photogenerated carriers and realize considerable solar-to-electric conversion ability, providing multifarious technologies to fabricate the electron extraction layer.
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http://dx.doi.org/10.1021/acsami.1c00688DOI Listing
March 2021

Research Progress in Organic Synthesis by Means of Photoelectrocatalysis.

Chem Rec 2021 Mar 3. Epub 2021 Mar 3.

Beijing Key Laboratory of Energy Conversion and Storage Materials, College of Chemistry, and Key Laboratory of Radiopharmaceuticals, Ministry of Education, Beijing Normal University, Beijing, 100875, P. R. China.

The rapid development of radical chemistry has spurred several innovative strategies for organic synthesis. The novel approaches for organic synthesis play a critical role in promoting and regulating the single-electron redox activity. Among them, photoelectrocatalysis (PEC) has attained considerable attention as the most promising strategy to convert organic compounds into fine chemicals. This review highlights the current progress in organic synthesis through PEC, including various catalytic reactions, catalyst systems and practical applications. The numerous catalytic reactions suffer the high overpotential and poor conversion efficiency, depending on the design of electrolyzers and the reaction mechanisms. We also considered the recent developments with special emphasis on scientific problems and efficient solutions, which enhance accessibility to utilize and further develop the photoelectrocatalytic technology for the specific chemical bonds formation and the fabrication of numerous catalytic systems.
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http://dx.doi.org/10.1002/tcr.202000186DOI Listing
March 2021

Strain Distribution and Drug Susceptibility of Invasive Fungal Infection in Clinical Patients With Systemic Internal Diseases.

Front Bioeng Biotechnol 2020 11;8:625024. Epub 2021 Feb 11.

Department of Dermatology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

Background: Patients with systemic internal diseases present high risks for invasive fungal infections, which results in increased morbidity and mortality. Identification of high-risk departments and susceptibility systems could help to reduce the infective rate clinically. Correct selection of sensitive anti-fungal drugs not only could improve the cure rate but also could reduce the adverse reactions and complications caused by long-term antifungal drug treatment, which can be especially important in patients with serious systemic diseases. Therefore, the distribution changes of invasive fungal strains in patients with systemic internal diseases and the choice of antifungal drugs in clinical practice should be updated.

Objective: This work aimed to investigate the incidence, strain distributions, and drug susceptibility of invasive fungal strains isolated from patients with systemic internal diseases.

Methods: Samples were collected from 9,430 patients who were diagnosed with internal diseases in our hospital from January to December 2018. We then cultured and identified the fungal strains using API 20C AUX. We performed drug sensitivity analysis the ATB Fungus-3 fungal susceptibility strip. Resistance was defined using the revised Clinical Laboratory Standardization Committee of United States breakpoints/epidemiological cutoff values to assign susceptibility or wild-type status to systemic antifungal agents.

Results: A total of 179 patients (49 female, 130 male) with fungal infection were included. The high-incidence departments were determined to be the respiratory department (34.64%), intensive care unit (ICU; 21.79%), and hepatology department (9.50%). The susceptible systems for infection were the respiratory tract (sputum, 68.72%, 123/179; secretion retained in the tracheal catheter, 3.35%, 6/179), urinary tract (urine, 9.50%, 17/179), and gastrointestinal tract (feces, 9.50%, 17/179). The major pathogens were (90.50%), (8.93%), and (0.56%). The infective candida subgroups were (70.95%), (6.15%), (5.59%), (3.91%), and (3.91%). The susceptibility of non- fungi for amphotericin B was 100.0%. The susceptibility rates of 5-fluorocytocine (5-FC) and voriconazole were 72.73 and 81.82%, respectively, for , 98.43 and 100% for , and 100% for both drugs for , , and . The susceptibility rates of fluconazole and itraconazole were 0 and 54.55%, respectively, for , 20 and 20% for , and 57.14 and 57.14% for . The resistance rate of for both fluconazole and itraconazole was 41.43%.

Conclusion: Patients in the respiratory department, ICU, and hepatology department presented high rates of invasive fungal infections and should include special attention during clinical treatment. The respiratory tract, urinary tract, and gastrointestinal tract were the susceptible systems. , especially , was the main pathogen. From the perspective of drug sensitivity, amphotericin B should be given priority in treating the non- fungi infection in patients with systemic internal diseases, while the susceptibility of invasive fungal strains to azoles was variant. These data might provide clinical evidence for the prevention and treatment of invasive fungal infection in patients with systemic internal diseases.
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http://dx.doi.org/10.3389/fbioe.2020.625024DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7906281PMC
February 2021

Corrigendum: Active Anaerobic Archaeal Methanotrophs in Recently Emerged Cold Seeps of Northern South China Sea.

Front Microbiol 2021 11;12:653239. Epub 2021 Feb 11.

Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou, China.

[This corrects the article DOI: 10.3389/fmicb.2020.612135.].
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http://dx.doi.org/10.3389/fmicb.2021.653239DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7906071PMC
February 2021