Publications by authors named "Tingting Tang"

267 Publications

Users' willingness to adopt health information in a social question-and-answer community: A cross-sectional study in China.

J Med Internet Res 2021 May 8. Epub 2021 May 8.

Medical Informatics college, Chongqing Medical University, No.1 Yixueyuan Road, Yuzhong District, Chongqing, CN.

Background: COVID-19 has spread around the world, increasing the public's need for health information in the process. Meanwhile, in the context of lockdowns and other measures to prevent virus spread, the Internet has surged as an online resource for health information. Under these conditions, social question-and-answer communities (SQACs) are playing an increasingly important role in improving public health literacy. There is great theoretical and practical significance in exploring the influencing factors of SQAC users' willingness to adopt health information.

Objective: The aim of this study is to establish an UTAUT extended model which could analyze influence factors of SQAC users' willingness to adopt health information. Particularly, we tried to test the moderating effect of different demographic characteristics on the variables' influences.

Methods: The study was conducted with the administration of an online questionnaire survey and the analysis of the responses from a final total of 598 valid questionnaires after invalid data were cleaned. Using structural equation modelling, the influencing factors of SQAC users' willingness to adopt health information were analysed. The moderating effects of variables were verified by hierarchical regression.

Results: Performance expectation (βPE=.282, P<.001), social influence (βSI=.238,P=.02) and facilitating conditions (βFC=.279,P=.002) positively affected users' willingness to adopt health information, whereas effort expectancy (P=.79) and perceived risk (P=.41) had no significant effects. Gender had a significant moderating effect in the structural equation model.

Conclusions: SQAC users' willingness to adopt health information was evidently affected by multiple factors such as performance expectation, social influence and facilitating conditions. The structural equation model proposed in this study has a good fitting situation and explanatory power of users' willingness to adopt health information. Suggestions are provided for the SQAC operators and health management agencies based on research results.

Clinicaltrial:
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http://dx.doi.org/10.2196/27811DOI Listing
May 2021

VE-cadherin-based matrix promoting the self-reconstruction of pro-vascularization microenvironments and endothelial differentiation of human mesenchymal stem cells.

J Mater Chem B 2021 Apr 6;9(15):3357-3370. Epub 2021 Apr 6.

The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Tianjin, 300071, China.

Regulating the secretion and endothelial differentiation of human mesenchymal stem cells (hMSCs) plays an important role in the vascularization in tissue engineering and regenerative medicine. In this study, a recombinant cadherin fusion protein consisting of a human vascular endothelial-cadherin extracellular domain and immunoglobulin IgG Fc region (hVE-cad-Fc) was developed as a bioartificial matrix for modulating hMSCs. The hVE-cad-Fc matrix significantly enhanced the secretion of angiogenic factors, activated the VE-cadherin-VEGFR2/FAK-AKT/PI3K signaling pathway in hMSCs, and promoted the endothelial differentiation of hMSCs even without extra VEGF. Furthermore, the hVE-cad-Fc matrix was applied for the surface modification of a poly (lactic-co-glycolic acid) (PLGA) porous scaffold, which significantly improved the hemocompatibility and vascularization of the PLGA scaffold in vivo. These results revealed that the hVE-cad-Fc matrix should be a superior bioartificial ECM for remodeling the pro-vascularization extracellular microenvironment by regulating the secretion of hMSCs, and showed great potential for the vascularization in tissue engineering.
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http://dx.doi.org/10.1039/d1tb00017aDOI Listing
April 2021

Evaluation of measles vaccination coverage in Lincang City, Yunnan Province, China.

Hum Vaccin Immunother 2021 Apr 13:1-8. Epub 2021 Apr 13.

Epidemiology and Biostatistics Unit, Faculty of Public Health, Dali University, Dali, China.

Lincang City in Yunnan Province on the China-Myanmar border, has reached the World Health Organization recommended coverage (95%) for measles-containing vaccine (MCV), but measles outbreaks still occur. We conducted a survey in Lincang City to determine the measles vaccination status of children on the China-Myanmar border. We used multistage sampling among children aged 8-83 months. Information on measles vaccination status was obtained from the child's vaccination certificate, and serum samples were tested using commercially available ELISA kits. A total of 938 children were surveyed. The vaccination coverage rate was 98.9% (95% CI: 98.2-99.6%) for measles-containing vaccine dose 1 (MCV1), and 95.8% (95% CI:94.9-96.7%) for measles-containing vaccine dose 2 (MCV2). The timely vaccination coverage rate was 52.0% (95% CI:48.8-55.2%) for MCV1, and 74.1% (95% CI: 82.9-89.0%) for MCV2. The timely-and-complete vaccination coverage rate was 41.0% (95% CI: 36.7-45.3%). The median delay period was 33 (95% CI: 27-39) days for MCV1, and 196 (95% CI: 146-246) days for MCV2. The seropositivity rate in children aged less than 7 years was 94.0% (95% CI: 92.5-95.5%) with a geometric mean titer of 1210.1 mIU/mL. The MCV coverage was high, but timely and timely-and-complete vaccination coverage were low and insufficient to prevent measles outbreaks. It is necessary to add the timely and timely-and-complete vaccination coverage as indicators of vaccination to provide a more complete picture of measles immunization status.
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http://dx.doi.org/10.1080/21645515.2021.1911215DOI Listing
April 2021

The Loss Surface Of Deep Linear Networks Viewed Through The Algebraic Geometry Lens.

IEEE Trans Pattern Anal Mach Intell 2021 Apr 6;PP. Epub 2021 Apr 6.

By using the viewpoint of modern computational algebraic geometry, we explore properties of the optimization landscapes of the deep linear neural network models. After clarifying on the various definitions of "flat" minima, we show that the geometrically flat minima, which are merely artifacts of residual continuous symmetries of the deep linear networks, can be straightforwardly removed by a generalized L_ regularization. Then, we establish upper bounds on the number of isolated stationary points of these networks with the help of algebraic geometry. Using these upper bounds and utilizing a numerical algebraic geometry method, we find stationary points for modest depth and matrix size. We show that in the presence of the non-zero regularization, deep linear networks indeed possess local minima which are not the global minima. We show that though the number of stationary points increases as the number of neurons (regularization parameter) increases (decreases), the number of higher index saddles are surprisingly rare.
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http://dx.doi.org/10.1109/TPAMI.2021.3071289DOI Listing
April 2021

Users' Willingness to Share Health Information in a Social Question-and-Answer Community: Cross-sectional Survey in China.

JMIR Med Inform 2021 Mar 30;9(3):e26265. Epub 2021 Mar 30.

College of Medical Informatics, Chongqing Medical University, Chongqing, China.

Background: Social question-and-answer communities play an increasingly important role in the dissemination of health information. It is important to identify influencing factors of user willingness to share health information to improve public health literacy.

Objective: This study explored influencing factors of social question-and-answer community users who share health information to provide reference for the construction of a high-quality health information sharing community.

Methods: A cross-sectional study was conducted through snowball sampling of 185 participants who are Zhihu users in China. A structural equation analysis was used to verify the interaction and influence of the strength between variables in the model. Hierarchical regression was also used to test the mediating effect in the model.

Results: Altruism (β=.264, P<.001), intrinsic reward (β=.260, P=.03), self-efficacy (β=.468, P<.001), and community influence (β=.277, P=.003) had a positive effect on users' willingness to share health information (WSHI). By contrast, extrinsic reward (β=-0.351, P<.001) had a negative effect. Self-efficacy also had a mediating effect (β=.147, 29.15%, 0.147/0.505) between community influence and WSHI.

Conclusions: The findings suggest that users' WSHI is influenced by many factors including altruism, self-efficacy, community influence, and intrinsic reward. Improving the social atmosphere of the platform is an effective method of encouraging users to share health information.
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http://dx.doi.org/10.2196/26265DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8075348PMC
March 2021

FOXP1 drives osteosarcoma development by repressing P21 and RB transcription downstream of P53.

Oncogene 2021 Apr 14;40(15):2785-2802. Epub 2021 Mar 14.

Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Osteosarcoma has a poor prognosis, and the poor understanding of the genetic drivers of osteosarcoma hinders further improvement in therapeutic approaches. Transcription factor forkhead box P1 (FOXP1) is a crucial modulator in skeletal development and aging. Here, we determined the role and regulatory mechanisms of FOXP1 in osteosarcoma. Higher FOXP1 expression correlated with malignancy in both osteosarcoma cell lines and clinical biopsies. FOXP1 overexpression and knockdown in osteosarcoma cell lines revealed that FOXP1 promoted proliferation, tumor sphere formation, migration and invasion, and inhibited anoikis. Mechanistically, FOXP1 acted as a repressor of P21 and RB (retinoblastoma protein) transcription, and directly interacted with the tumor suppressor p53 to inhibit its activity. Extracellular signal-regulated kinase/c-Jun N-terminal kinase (ERK/JNK) signaling and c-JUN/c-FOS transcription factors were found to be upstream activators of FOXP1. Moreover, FOXP1 silencing via lentivirus or adeno-associated virus (AAV)-mediated delivery of shRNA suppressed osteosarcoma development and progression in cell-derived and patient-derived xenograft animal models. Taken together, we demonstrate that FOXP1, which is transactivated by ERK/JNK-c-JUN/c-FOS, drives osteosarcoma development by regulating the p53-P21/RB signaling cascade, suggesting that FOXP1 is a potential target for osteosarcoma therapy.
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http://dx.doi.org/10.1038/s41388-021-01742-4DOI Listing
April 2021

Inhibition of fibroblast IL-6 production by ACKR4 deletion alleviates cardiac remodeling after myocardial infarction.

Biochem Biophys Res Commun 2021 Apr 18;547:139-147. Epub 2021 Feb 18.

Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China; Key Laboratory of Biological Targeted Therapy of the Ministry of Education, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. Electronic address:

Fibrotic scarring is tightly linked to the development of heart failure in patients with post-myocardial infarction (MI). Atypical chemokine receptor 4 (ACKR4) can eliminate chemokines, such as C-C chemokine ligand 21 (CCL21), which is independently associated with heart failure mortality. However, the role of ACKR4 in the heart during MI is unrevealed. This study aimed to determine whether ACKR4 modulates cardiac remodeling following MI and to illuminate the potential molecular mechanisms. The expression of ACKR4 was upregulated in the border/infarct area, and ACKR4 was predominantly expressed in cardiac fibroblasts (CFs). Knockout of ACKR4 protected against adverse ventricular remodeling in mice post-MI. These protective effects of ACKR4 deficiency were independent of dendritic cell immune response but could be attributed to downregulated CF-derived IL-6, affecting CF proliferation and endothelial cell (EC) functions, which consequently inhibited cardiac fibrosis. ACKR4 promoted IL-6 generation and proliferation of CFs. Besides, ACKR4 induced endothelial-to-mesenchymal transition (EndMT) in ECs through IL-6 paracrine effect. The p38 MAPK/NF-κB signaling pathway was involved in ACKR4 facilitated IL-6 generation. Moreover, ACKR4 overexpression in vivo via AAV9 carrying a periostin promoter aggravated heart functional impairment post-MI, which was abolished by IL-6 neutralizing antibody. Therefore, our study established a novel link between ACKR4 and IL-6 post-MI, indicating that ACKR4 may be a novel therapeutic target to ameliorate cardiac remodeling.
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http://dx.doi.org/10.1016/j.bbrc.2021.02.013DOI Listing
April 2021

Structural basis for ligand recognition of the neuropeptide Y Y receptor.

Nat Commun 2021 02 2;12(1):737. Epub 2021 Feb 2.

CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zuchongzhi Road, Pudong, Shanghai, 201203, China.

The human neuropeptide Y (NPY) Y receptor (YR) plays essential roles in food intake, bone formation and mood regulation, and has been considered an important drug target for obesity and anxiety. However, development of drugs targeting YR remains challenging with no success in clinical application yet. Here, we report the crystal structure of YR bound to a selective antagonist JNJ-31020028 at 2.8 Å resolution. The structure reveals molecular details of the ligand-binding mode of YR. Combined with mutagenesis studies, the YR structure provides insights into key factors that define antagonistic activity of diverse antagonists. Comparison with the previously determined antagonist-bound YR structures identified receptor-ligand interactions that play different roles in modulating receptor activation and mediating ligand selectivity. These findings deepen our understanding about molecular mechanisms of ligand recognition and subtype specificity of NPY receptors, and would enable structure-based drug design.
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http://dx.doi.org/10.1038/s41467-021-21030-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7854658PMC
February 2021

Intention to accept pertussis vaccination among Chinese people older than age 5.

Hum Vaccin Immunother 2021 Jun 15;17(6):1686-1692. Epub 2021 Jan 15.

Department of Immunization Program, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, P.R. China.

: The intention of Chinese people older than age 5 to accept the pertussis vaccination is unknown, which is important for the future design of an effective pertussis vaccination program in China.: We conducted a cross-sectional survey among people older than age 5 in China. A 24-item questionnaire was used to explore the determinants of intention to accept a pertussis vaccination, and for children and adolescents (≦15 years old), their guardian was required to help answer the questionnaire on their behalf. Univariate and multivariate logistic regression was used to analyze the influencing factors of intention.: A total of 3,041 individuals participated in our survey and 3025 completed the questionnaire, among which 1938 (64.07%) reported a positive intention to be vaccinated. A multiple logistic regression analysis revealed that the main positive factors for the intention to accept pertussis vaccination were younger age (Odd ratio [OR] 0.838, < .001), higher educational attainment (OR 1.171, = .002), no hospitalization because of the most recent cough (OR 2.468, < .001), awareness about pertussis (OR 1.958, < .001), and consideration of pertussis vaccination to be safe (OR 1.450, = .026).: There is a relatively high level of intention to receive the pertussis vaccine among people older than age 5 in China. Future pertussis vaccination strategies geared at them should consider focusing on middle-aged and older individuals and those with a low education as well as strengthen the promotion of disease characteristics, vaccine effectiveness, and safety.
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http://dx.doi.org/10.1080/21645515.2020.1849517DOI Listing
June 2021

Effect of Prandtl Number on Mixed Convective Heat Transfer from a Porous Cylinder in the Steady Flow Regime.

Entropy (Basel) 2020 Feb 6;22(2). Epub 2020 Feb 6.

Shenzhen Key Laboratory of Complex Aerospace Flows, Department of Mechanics and Aerospace Engineering, Southern University of Science and Technology, Shenzhen 518055, China.

The effect of the Prandtl number () on the flow and heat transfer from a porous circular cylinder with internal heat generation in the mixed convection regime is numerically investigated. The steady flow regime is considered over the ranges of the Reynolds number (), Darcy number (), and Richardson number (), varying from 5 to 40, 10 to 10, and 0 to 2, respectively. The wake structure, the temperature distribution, and the heat transfer rate are discussed. Besides precipitating the growth of the recirculating wake, the Prandtl number is found to have a significant impact on the thermal characteristics. The concave isotherms, resembling a saddle-shaped structure, occur behind the cylinder at larger , resulting in swells of the isotherms pairing off at the lateral sides. These swells are found to have a negative effect on heat transfer owing to a relatively smaller temperature gradient there. Then, the heat transfer rate in terms of the local Nusselt number () and enhancement ratio () is calculated, which is closely related to , , , and The local minimum heat transfer rate along the cylinder surface is found at the position where the swells of the isotherms form.
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http://dx.doi.org/10.3390/e22020184DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7516614PMC
February 2020

A 3D-bioprinted scaffold with doxycycline-controlled BMP2-expressing cells for inducing bone regeneration and inhibiting bacterial infection.

Bioact Mater 2021 May 10;6(5):1318-1329. Epub 2020 Nov 10.

Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, 200011, China.

Large bone defects face a high risk of pathogen exposure due to open wounds, which leads to high infection rates and delayed bone union. To promote successful repair of infectious bone defects, fabrication of a scaffold with dual functions of osteo-induction and bacterial inhibition is required. This study describes creation of an engineered progenitor cell line (C3H10T1/2) capable of doxycycline (DOX)-mediated release of bone morphogenetic protein-2 (BMP2). Three-dimensional bioprinting technology enabled creation of scaffolds, comprising polycaprolactone/mesoporous bioactive glass/DOX and bioink, containing these engineered cells. and experiments confirmed that the scaffold could actively secrete BMP2 to significantly promote osteoblast differentiation and induce ectopic bone formation. Additionally, the scaffold exhibited broad-spectrum antibacterial capacity, thereby ensuring the survival of embedded engineered cells when facing high risk of infection. These findings demonstrated the efficacy of this bioprinted scaffold to release BMP2 in a controlled manner and prevent the occurrence of infection; thus, showing its potential for repairing infectious bone defects.
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http://dx.doi.org/10.1016/j.bioactmat.2020.10.022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7658329PMC
May 2021

Molecular basis and therapeutic implications of CD40/CD40L immune checkpoint.

Pharmacol Ther 2021 Mar 20;219:107709. Epub 2020 Oct 20.

Center for Metabolic Disease Research, Lewis Katz School of Medicine, Temple University, Philadelphia, PA; Department of Microbiology and Immunology, Lewis Katz School of Medicine, Temple University, Philadelphia, PA. Electronic address:

The CD40 receptor and its ligand CD40L is one of the most critical molecular pairs of the stimulatory immune checkpoints. Both CD40 and CD40L have a membrane form and a soluble form generated by proteolytic cleavage or alternative splicing. CD40 and CD40L are widely expressed in various types of cells, among which B cells and myeloid cells constitutively express high levels of CD40, and T cells and platelets express high levels of CD40L upon activation. CD40L self-assembles into functional trimers which induce CD40 trimerization and downstream signaling. The canonical CD40/CD40L signaling is mediated by recruitment of TRAFs and NF-κB activation, which is supplemented by signal pathways such as PI3K/AKT, MAPKs and JAK3/STATs. CD40/CD40L immune checkpoint leads to activation of both innate and adaptive immune cells via two-way signaling. CD40/CD40L interaction also participates in regulating thrombosis, tissue inflammation, hematopoiesis and tumor cell fate. Because of its essential role in immune activation, CD40/CD40L interaction has been regarded as an attractive immunotherapy target. In recent years, significant advance has been made in CD40/CD40L-targeted therapy. Various types of agents, including agonistic/antagonistic monoclonal antibodies, cellular vaccines, adenoviral vectors and protein antagonist, have been developed and evaluated in early-stage clinical trials for treating malignancies, autoimmune diseases and allograft rejection. In general, these agents have demonstrated favorable safety and some of them show promising clinical efficacy. The mechanisms of benefits include immune cell activation and tumor cell lysis/apoptosis in malignancies, or immune cell inactivation in autoimmune diseases and allograft rejection. This review provides a comprehensive overview of the structure, processing, cellular expression pattern, signaling and effector function of CD40/CD40L checkpoint molecules. In addition, we summarize the progress, targeted diseases and outcomes of current ongoing and completed clinical trials of CD40/CD40L-targeted therapy.
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http://dx.doi.org/10.1016/j.pharmthera.2020.107709DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7886970PMC
March 2021

A Unique Population of Regulatory T Cells in Heart Potentiates Cardiac Protection From Myocardial Infarction.

Circulation 2020 Nov 28;142(20):1956-1973. Epub 2020 Sep 28.

Department of Cardiology, Union Hospital, and Key Laboratory of Biological Targeted Therapy of the Ministry of Education (N.X., Y. Lu, M.G., N.L., M.L., J.J., Z.Z., J.L., D.L., T.T., B.L., S.N., M.Z., M.L., Y. Liao, X.C.), Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: Regulatory T cells (Tregs), traditionally recognized as potent suppressors of immune response, are increasingly attracting attention because of a second major function: residing in parenchymal tissues and maintaining local homeostasis. However, the existence, unique phenotype, and function of so-called tissue Tregs in the heart remain unclear.

Methods: In mouse models of myocardial infarction (MI), myocardial ischemia/reperfusion injury, or cardiac cryoinjury, the dynamic accumulation of Tregs in the injured myocardium was monitored. The bulk RNA sequencing was performed to analyze the transcriptomic characteristics of Tregs from the injured myocardium after MI or ischemia/reperfusion injury. Photoconversion, parabiosis, single-cell T-cell receptor sequencing, and adoptive transfer were applied to determine the source of heart Tregs. The involvement of the interleukin-33/suppression of tumorigenicity 2 axis and Sparc (secreted acidic cysteine-rich glycoprotein), a molecule upregulated in heart Tregs, was further evaluated in functional assays.

Results: We showed that Tregs were highly enriched in the myocardium of MI, ischemia/reperfusion injury, and cryoinjury mice. Transcriptomic data revealed that Tregs isolated from the injured hearts had plenty of differentially expressed transcripts in comparison with their lymphoid counterparts, including heart-draining lymphoid nodes, with a phenotype of promoting infarct repair, indicating a unique characteristic. The heart Tregs were accumulated mainly because of recruitment from the circulating Treg pool, whereas local proliferation also contributed to their expansion. Moreover, a remarkable case of repeatedly detected T-cell receptor of heart Tregs, more than that of spleen Tregs, suggests a model of clonal expansion. Besides, HeliosNrp-1 phenotype proved the mainly thymic origin of heart Tregs, with a small contribution of phenotypic conversion of conventional CD4 T cells, proved by the analysis of T-cell receptor repertoires and conventional CD4 T cells adoptive transfer experiments. The interleukin-33/suppression of tumorigenicity 2 axis was essential for sustaining heart Treg populations. Last, we demonstrated that Sparc, which was highly expressed by heart Tregs, acted as a critical factor to protect the heart against MI by increasing collagen content and boosting maturation in the infarct zone.

Conclusions: We identified and characterized a phenotypically and functionally unique population of heart Tregs that may lay the foundation to harness Tregs for cardioprotection in MI and other cardiac diseases.
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http://dx.doi.org/10.1161/CIRCULATIONAHA.120.046789DOI Listing
November 2020

Cerium Oxide Nanoparticles Regulate Osteoclast Differentiation Bidirectionally by Modulating the Cellular Production of Reactive Oxygen Species.

Int J Nanomedicine 2020 25;15:6355-6372. Epub 2020 Aug 25.

Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, People's Republic of China.

Background: Cerium oxide nanoparticles (CeONPs) are potent scavengers of cellular reactive oxygen species (ROS). Their antioxidant properties make CeONPs promising therapeutic agents for bone diseases and bone tissue engineering. However, the effects of CeONPs on intracellular ROS production in osteoclasts (OCs) are still unclear. Numerous studies have reported that intracellular ROS are essential for osteoclastogenesis. The aim of this study was to explore the effects of CeONPs on osteoclast differentiation and the potential underlying mechanisms.

Methods: The bidirectional modulation of osteoclast differentiation by CeONPs was explored by different methods, such as fluorescence microscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), quantitative real-time polymerase chain reaction (qRT-PCR), and Western blotting. The cytotoxic and proapoptotic effects of CeONPs were detected by cell counting kit (CCK-8) assay, TdT-mediated dUTP nick-end labeling (TUNEL) assay, and flow cytometry.

Results: The results of this study demonstrated that although CeONPs were capable of scavenging ROS in acellular environments, they facilitated the production of ROS in the acidic cellular environment during receptor activator of nuclear factor kappa-Β ligand (RANKL)-dependent osteoclast differentiation of bone marrow-derived macrophages (BMMs). CeONPs at lower concentrations (4.0 µg/mL to 8.0 µg/mL) promoted osteoclast formation, as shown by increased expression of and , F-actin ring formation and bone resorption. However, at higher concentrations (greater than 16.0 µg/mL), CeONPs inhibited osteoclast differentiation and promoted apoptosis of BMMs by reducing Bcl2 expression and increasing the expression of cleaved caspase-3, which may be due to the overproduction of ROS.

Conclusion: This study demonstrates that CeONPs facilitate osteoclast formation at lower concentrations while inhibiting osteoclastogenesis in vitro by inducing the apoptosis of BMMs at higher concentrations by modulating cellular ROS levels.
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http://dx.doi.org/10.2147/IJN.S257741DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7457858PMC
October 2020

Correlation of sarcopenia and depressive mood in older community dwellers: a cross-sectional observational study in China.

BMJ Open 2020 09 1;10(9):e038089. Epub 2020 Sep 1.

Department of Geratology, the First Hospital Affiliated to Nanjing Medical University, Nanjing, China

Objective: Whether sarcopenia is detrimental to depression is still controversial, which may be due to the three components of the sarcopenia. Our objective was to define the correlation between depression and sarcopenia in older Chinese community dwellers.

Design: The study has a cross-sectional design.

Setting: The study was conducted in Jiangsu, China.

Participants: A total of 101 men and 149 women aged 60 years or older were recruited.

Outcome Measures: Lean tissue mass was measured by dual-energy X-ray absorptiometry. Muscle strength in the upper and lower limbs was measured by a handheld dynamometer and a chair stand test, respectively. Physical performance was assessed by gait speed and standing balance tests. Depressive mood was assessed using the Geriatric Depression Scale-30 (range 0-30).

Results: Participants in the sarcopenia group had a higher mean depression score than the normal group (p=0.002). Pearson's correlation analysis showed that depression was negatively associated with muscle strength (handgrip strength: R=-0.170, p=0.028 for women, R=-0.196, p=0.048 for men; chair stand test performance: R=0.252, p=0.002 for women, R=0.311, p=0.001 for men) and physical performance (gait speed: R=-0.200, p=0.009, standing balance test performance: R=-0.224, p=0.006, Short Physical Performance Battery (SPPB): R=-0.218, p=0.007 for women; SPPB: R=-0.252, p=0.01 for men). Multiple linear regression models revealed that depressive mood was inversely associated with chair stand test (β0.325, p<0.001), gait speed (β-0.009, p=0.041) and standing balance test (β=-0.24, p=0.016) after adjusting for confounding factors, while no significant correlation was observed between depressive mood and muscle mass.

Conclusion: The diagnostic components of sarcopenia-strength of the leg muscles (chair stand test) and physical performance (gait speed and standing balance test)-were associated with depressive mood.
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http://dx.doi.org/10.1136/bmjopen-2020-038089DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7467510PMC
September 2020

Nrf2 mitigates prolonged PM2.5 exposure-triggered liver inflammation by positively regulating SIKE activity: Protection by Juglanin.

Redox Biol 2020 09 17;36:101645. Epub 2020 Jul 17.

Chongqing Key Laboratory of Medicinal Resources in the Three Gorges Reservoir Region, School of Biological and Chemical Engineering, Chongqing University of Education, Chongqing, 400067, PR China; Research Center of Brain Intellectual Promotion and Development for Children Aged 0-6 Years, Chongqing University of Education, Chongqing, 400067, PR China; Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, 400030, China. Electronic address:

Air pollution containing particulate matter (PM) less than 2.5 μm (PM) plays an essential role in regulating hepatic disease. However, its molecular mechanism is not yet clear, lacking effective therapeutic strategies. In this study, we attempted to investigate the effects and mechanisms of PM exposure on hepatic injury by the in vitro and in vivo experiments. At first, we found that PM incubation led to a significant reduction of nuclear factor erythroid-derived 2-related factor 2 (Nrf2), along with markedly reduced expression of different anti-oxidants. Notably, suppressor of IKKε (SIKE), known as a negative regulator of the interferon pathway, was decreased in PM-incubated cells, accompanied with increased activation of TANK-binding kinase 1 (TBK1) and nuclear factor-κB (NF-κB). The in vitro studies showed that Nrf2 positively regulated SIKE expression under the conditions with or without PM. After PM treatment, Nrf2 knockdown further accelerated SIEK decrease and TBK1/NF-κB activation, and opposite results were observed in cells with Nrf2 over-expression. Subsequently, the gene loss- and gain-function analysis demonstrated that SIKE deficiency further aggravated inflammation and TBK1/NF-κB activation caused by PM, which could be abrogated by SIKE over-expression. Importantly, SIKE-alleviated inflammation was mainly dependent on TBK1 activation. The in vivo studies confirmed that SIKE- and Nrf2-knockout mice showed significantly accelerated hepatic injury after long-term PM exposure through reducing inflammatory response and oxidative stress. Juglanin (Jug), mainly isolated from Polygonum aviculare, exhibits anti-inflammatory and anti-oxidant effects. We found that Jug could increase Nrf2 activation, and then up-regulated SIKE in cells and liver tissues, mitigating PM-induced liver injury. Together, all these data demonstrated that Nrf2 might positively meditate SIKE to inhibit inflammatory and oxidative damage, ameliorating PM-induced liver injury. Jug could be considered as an effective therapeutic strategy against this disease by improving Nrf2/SIKE signaling pathway.
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http://dx.doi.org/10.1016/j.redox.2020.101645DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7387847PMC
September 2020

A sandwich electrochemiluminescent assay for determination of concanavalin A with triple signal amplification based on MoSNF@MWCNTs modified electrode and Zn-MOF encapsulated luminol.

Mikrochim Acta 2020 08 28;187(9):523. Epub 2020 Aug 28.

Key Laboratory of Synthetic and Natural Functional Molecule Chemistry (Ministry of Education), College of Chemistry & Materials Science, Northwest University, Xi'an, 710069, People's Republic of China.

An ultrasensitive sandwich electrochemiluminescence (ECL) biosensor was designed for determination of concanavalin A (ConA) through the specific carbohydrate-ConA interactions. Three-dimensional porous metal-organic framework (Zn-MOF) was synthesized, which loaded a large amount of luminescent reagents as luminol by encapsulating into its pores to form Zn-MOF@luminol complex. Interestingly, Zn-MOF also acted as the coreactant accelerator in the luminol-HO ECL system. This Zn-MOF@luminol complex was used as the signal probe to achieve a super strong and stable ECL signal. In addition, three-dimensional hierarchical molybdenum disulfide nanoflower and multiwalled carbon nanotubes complex (MoSNF@MWCNTs) with peroxidase-mimicking enzyme property were used as a substrate to modify the glassy carbon electrode to further enhance the ECL signal of luminol by promoting decomposition of HO into reactive oxygen species (ROSs). In addition to the horseradish peroxidase (HRP) catalysis effect on the luminol ECL signal, a triple amplified ConA sandwich ECL sensor with high sensitivity sensor was constructed. The linear range for ConA detection was from 0.5 pg/mL to 100 ng/mL with a detection limit of 0.3 pg/mL (S/N = 3). The recovery test for ConA in human serum samples was performed with satisfactory results. Graphical abstract.
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http://dx.doi.org/10.1007/s00604-020-04472-8DOI Listing
August 2020

Role of microRNA-33a in malignant cells.

Oncol Lett 2020 Sep 9;20(3):2537-2556. Epub 2020 Jul 9.

Key Laboratory for Research and Development of Natural Drugs of Guangdong Province, School of Pharmacy, Guangdong Medical University, Dongguan, Guangdong 523808, P.R. China.

Cancer causes most of the mortality and morbidity worldwide, with a significant increase in incidence during recent years. MicroRNAs (miRNAs/miRs) are non-coding small RNAs capable of regulating gene expression. They regulate crucial cellular processes, including proliferation, differentiation, metastasis and apoptosis. Therefore, abnormal miRNA expression is associated with multiple diseases, including cancer. There are two types of cancer-associated miRNAs, oncogenic and tumor suppressor miRNAs, depending on their roles and expression patterns in cancer. Accordingly, miRNAs are considered to be targets for cancer prevention and treatment. miR-33a controls cellular cholesterol uptake and synthesis, which are both closely associated with carcinogenesis. The present review thoroughly describes the roles of miR-33a in more than a dozen types of cancer and the underlying mechanisms. Accordingly, the present review may serve as a guide for researchers studying the involvement of miR-33a in diverse cancer settings.
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http://dx.doi.org/10.3892/ol.2020.11835DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7399786PMC
September 2020

Algae removal performance of UV-radiation-enhanced coagulation for two representative algal species.

Sci Total Environ 2020 Nov 19;745:141013. Epub 2020 Jul 19.

Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, China.

Algal blooms severely impact the ecological environment and human health, as well as drinking water supplies and treatment systems. This study investigated UV-radiation-enhanced aluminum (Al)-based coagulation for the removal of two representative algal species (Microcystis aeruginosa and Cyclotella sp.) which are responsible for most fresh water algal bloom in different seasons. The results demonstrated that the UV-Al process can enhance algae removal, and simultaneously control algal organic matter (AOM) release. Comparing with Microcystis aeruginosa, Cyclotella sp. was more sensitive to UV irradiation and its activity was severely inhibited by 240 s of UV irradiation; intracellular reactive oxygen species (ROS) increased sharply then decreased rapidly, and SEM images showed cell walls exhibited substantial compression. UV irradiation decreased the zeta potential, which might have contributed to algae removal. Approximately 93.5% of Microcystis aeruginosa cells and 91.4% of Cyclotella sp. cells were removed after 240 s of UV irradiation with 0.4 mmol/L Al. The MCs concentrations after Al coagulation were low (<100 ng/L). The DOC of Microcystis aeruginosa and Cyclotella sp. was also lower (1.2 and 1.6 mg/L, respectively) than the national standard level after UV-Al process. This study highlights the practical application of UV irradiation for enhancing algae removal and simultaneously controlling AOM release in water treatment plants, which is a simple and promising technology. This result also indicates that the water treatment parameters should be adjusted according to the algae species present in different seasons, especially for diatom which needs low UV irradiation and Al dosage.
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http://dx.doi.org/10.1016/j.scitotenv.2020.141013DOI Listing
November 2020

Role of PIWI-interacting RNAs on cell survival: Proliferation, apoptosis, and cycle.

IUBMB Life 2020 09 22;72(9):1870-1878. Epub 2020 Jul 22.

Pathophysiology Department, Institute of Cardiovascular Disease, Key Laboratory for Arteriosclerology of Hunan Province, Hunan International Scientific and Technological Cooperation Base of Arteriosclerotic disease, University of South China, Hengyang, China.

PIWI-interacting RNAs (piRNAs) are a kind of non-coding small RNAs, which play a biological role by specifically binding to PIWI proteins. Studies have demonstrated that piRNAs play a significant role in germline cell growth by repressing transposable elements, especially in the regulation of DNA methylation. Recently increasing evidence revealed that piRNAs involved in the regulation of cell proliferation, apoptosis, and cycle; however, the mechanism of piRNAs is unclear. This review summarizes the research progress regarding the roles of piRNAs in the cell proliferation, apoptosis, and cycle.
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http://dx.doi.org/10.1002/iub.2332DOI Listing
September 2020

Low muscle mass impairs fat utilization during light-intensity exercise in Chinese men.

Ann Palliat Med 2020 Jul 13;9(4):1453-1461. Epub 2020 Jul 13.

Department of Geratology, the First Hospital Affiliated to Nanjing Medical University, Nanjing, China.

Background: Age-related loss of the muscle mass is linked to obesity and diabetes because the muscle is the major site for fat oxidation which is influenced by exercise. We aimed to explore the association between fat oxidation rates (FORs) and exercise intensity during age-related muscle loss.

Methods: A total of 224 healthy Chinese men aged 23-92 years were recruited. Fat and lean tissue masses were measured by DXA. The subjects were also tested using graded incremental exercise with an increased intensity of 10 W until maximal fat oxidation rate (Fatmax) was achieved. Real-time contribution (%) of fat to total energy expenditure were determined using indirect calorimetry.

Results: We found that appendicular skeletal muscle mass/weight (ASM/Wt) decreased during ageing and positively correlated with FORs during low-intensity exercise. In multiple linear regression models, FORs was positively related with ASM/Wt (β =0.446, P=0.0091) but negatively associated with exercise intensity (β =-0.573, P<0.0001), whereas fat oxidation rate did not show any association with age. Moreover, there was a significantly negative correlation between ASM/Wt and Fatmax.

Conclusions: Our findings highlight the importance of muscle mass in fat utilization during low-intensity exercise. A higher exercise intensity indicated by Fatmax is recommended for improving fat oxidation in Chinese men with decreased muscle mass.
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http://dx.doi.org/10.21037/apm-20-611DOI Listing
July 2020

Bioprinting of an osteocyte network for biomimetic mineralization.

Biofabrication 2020 07 29;12(4):045013. Epub 2020 Jul 29.

Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China. These authors contributed equally to this work.

Osteocytes, essential regulators of bone homeostasis, are embedded in the mineralized bone matrix. Given the spatial arrangement of osteocytes, bioprinting represents an ideal method to biofabricate a 3D osteocyte network with a suitable surrounding matrix similar to native bone tissue. Here, we reported a 3D bioprinted osteocyte-laden hydrogel for biomimetic mineralization in vitro with exceptional shape fidelity, a high cell density (10 cells per ml) and high cell viability (85%-90%). The bioinks were composed of biomimetic modified biopolymers, namely, gelatine methacrylamide (GelMA) and hyaluronic acid methacrylate (HAMA), with or without type I collagen. The osteocyte-laden constructs were printed and cultured in mineralization induction media. After 28 d, increased dendritic cell connections and enhanced mineralized matrix production were observed after the addition of type I collagen. These results were further confirmed by the expression of osteocyte-related genes, markers of osteocyte morphology (Connexin43 and E11/Podoplanin), markers of mineralization (dentin matrix acidic phosphoprotein 1 (Dmp1)) and the cellular response to parathyroid hormone (PTH). Moreover, the 3D bioprinting constructs outperformed the 2D monolayer culture and they were at least comparable to 3D casted hydrogels in mimicking the natural osteocyte phenotype. All results indicated that the 3D bioprinting osteocyte network shows promise for mechanistic studies and pharmaceutical screening in vitro.
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http://dx.doi.org/10.1088/1758-5090/aba1d0DOI Listing
July 2020

Intestinal microbiota-farnesoid X receptor axis in metabolic diseases.

Clin Chim Acta 2020 Oct 4;509:167-171. Epub 2020 Jun 4.

Pathophysiology Department, Institute of Cardiovascular Disease, Key Laboratory for Arteriosclerology of Hunan Province, Hunan International Scientific and Technological Cooperation Base of Arteriosclerotic Disease, University of South China, Hengyang City, Hunan Province 421001, PR China. Electronic address:

Studies have demonstrated that intestinal microbiota is associated with various metabolic diseases including obesity, nonalcoholic fatty liver, and insulin resistance. Farnesoid X receptor (FXR), also known as the bile acid receptor, belongs to the nuclear receptor superfamily, which is involved in the regulation of bile acid, glucose, and lipid metabolism. Researchers have found that intestinal microbiota can regulate FXR activity by affecting bile acid composition, and then regulate the balance of in vivo metabolism. The intestinal microbiota -FXR axis may be an ideal drug target for metabolic diseases. This review summarizes the latest research on the intestinal microbiota /FXR axis, hoping to provide a theoretical basis for further research and clinical application.
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http://dx.doi.org/10.1016/j.cca.2020.06.006DOI Listing
October 2020

Removal of cyanobacteria and control of algal organic matter by simultaneous oxidation and coagulation - comparing the HO/Fe(II) and HO/Fe(III) processes.

Sci Total Environ 2020 Jun 29;720:137653. Epub 2020 Feb 29.

Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, China. Electronic address:

Cyanobacterial blooms in drinking water are worldwide concern. It is known that pre-oxidation enhanced coagulation can be more efficient at removing algae than traditional coagulation. However, its application is hindered by high oxidant/coagulant consumption and the resultant potential health risk, in the form of algal organic matter (AOM) released during oxidation. To remove the cyanobacteria and meanwhile ensure cell integrity, HO/Fe(II) and HO/Fe(III), which have been widely used to degrade organic pollutants in waters, are proposed in this study. The removal efficiency of Microcystis aeruginosa (M. aeruginosa) under various oxidant/coagulant dosages, AOM release and cell integrity, as well as floc formation and morphology were investigated with these simultaneous oxidation/coagulation processes. The results show that the removal efficiency was higher than 95% with HO/Fe(II) and HO/Fe(III) under 100 μmol/L HO and Fe. In addition, neither method was found to damage the algal cells in 50-200 μmol/L HO dosing concentrations. It was also found that AOM, including microcystins (MCs), was well controlled owing to the oxidation of HO or hydroxyl radicals, and in-situ Fe(III) settled down the cells in the processes. Compared with HO/Fe(II), HO/Fe(III) could remove algae efficiently and control AOM release with lower HO (50 μmol/L) and Fe(III) (80 μmol/L) dosages, which suggests that a low chemical consumption is suitable for this simultaneous oxidation/coagulation processes. This is a promising technology for the removal of algae from drinking water in a clean, economical way.
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http://dx.doi.org/10.1016/j.scitotenv.2020.137653DOI Listing
June 2020

The purification, structural characterization and antidiabetic activity of a polysaccharide from Anoectochilus roxburghii.

Food Funct 2020 Apr 21;11(4):3730-3740. Epub 2020 Apr 21.

College of Food Science, Sichuan Agricultural University, Yaan 625014, China.

Anoectochilus roxburghii, a traditional Chinese medicinal herb, has been widely used for treating numerous chronic diseases. In this study, a polysaccharide from A. roxburghii (ARPs-p) was purified by anion exchange and size exclusion chromatography. The structural characteristics of ARPs-p were systematically investigated for the first time via numerous chromatographic techniques, periodic acid oxidation, Smith degradation, methylation analysis, FTIR spectroscopy and 1D/2D NMR spectroscopy. The results showed that ARPs-p is a heteropolysaccharide with a molecular weight of 97 kDa; it consists of 97.75% glucose, 1.2% galactose and trace amounts of galacturonic acid, and its backbone structure is composed of →3)-β-d-Glcp-(1→ with some branching points at O-6 linked to non-reducing end units or 6-O-linked Glcp units. Furthermore, streptozotocin (STZ)-induced diabetic mouse experiments suggested that ARPs-p has excellent antihyperglycemic, antioxidant and antihyperlipidemic activities, in which 1,3-β-d-glucan, the main component of ARPs-p, plays a vital role.
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http://dx.doi.org/10.1039/c9fo00860hDOI Listing
April 2020

Prediction Network of Metamaterial with Split Ring Resonator Based on Deep Learning.

Nanoscale Res Lett 2020 Apr 15;15(1):83. Epub 2020 Apr 15.

Chengdu University of Information Technology, Chengdu, 610225, China.

The introduction of "metamaterials" has had a profound impact on several fields, including electromagnetics. Designing a metamaterial's structure on demand, however, is still an extremely time-consuming process. As an efficient machine learning method, deep learning has been widely used for data classification and regression in recent years and in fact shown good generalization performance. We have built a deep neural network for on-demand design. With the required reflectance as input, the parameters of the structure are automatically calculated and then output to achieve the purpose of designing on demand. Our network has achieved low mean square errors (MSE), with MSE of 0.005 on both the training and test sets. The results indicate that using deep learning to train the data, the trained model can more accurately guide the design of the structure, thereby speeding up the design process. Compared with the traditional design process, using deep learning to guide the design of metamaterials can achieve faster, more accurate, and more convenient purposes.
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http://dx.doi.org/10.1186/s11671-020-03319-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7158974PMC
April 2020

Improved antibacterial properties of collagen I/hyaluronic acid/quaternized chitosan multilayer modified titanium coatings with both contact-killing and release-killing functions.

J Mater Chem B 2019 03 18;7(11):1951-1961. Epub 2019 Feb 18.

Shanghai Key Laboratory of Orthopedic Implants, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhizaoju Road, Shanghai, China.

Implant infection is one of the most severe complications after orthopedic surgery. The construction of an antibacterial coating on orthopedic implants with release-killing or contact-killing is one of the most efficient strategies to prevent implant-related infections. Here we reported a hydroxypropyltrimethyl ammonium chloride chitosan (HACC) based multilayer modified plasma-sprayed porous titanium coating generated via the layer-by-layer covalent-immobilized method. We demonstrated that the multilayer coating inhibited the colonization and biofilm formation of several bacterial strains, including Staphylococcus aureus (ATCC 25923), methicillin-resistant Staphylococcus aureus (MSRA, ATCC 43300) and clinical isolates of methicillin-resistant Staphylococcus epidermidis (MRSE 287), in vitro. HACC in the multilayer was released slowly with the degradation of the coating under the action of collagenase, further killing the planktonic bacteria, while the remaining HACC could kill the colonized bacteria. In a rat model of femur implants, the HACC-based multilayer-modified TCs effectively controlled the infection caused by MRSA and prevented bone destruction. Therefore, the HACC-based multilayer modified TCs with multiple antimicrobial properties could be a new potential ideal surface modification strategy to prevent implant associated infections.
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http://dx.doi.org/10.1039/c8tb02425aDOI Listing
March 2019

Proteoglycan 4 predicts tribological properties of repaired cartilage tissue.

Theranostics 2020 22;10(6):2538-2552. Epub 2020 Jan 22.

Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedics, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China.

: One of the essential requirements in maintaining the normal joint motor function is the perfect tribological property of the articular cartilage. Many cartilage regeneration strategies have been developed for treatment in early stages of osteoarthritis, but there is little information on how repaired articular cartilage regains durability. The identification of biomarkers that can predict wear resistant property is critical to advancing the success of cartilage regeneration therapies. Proteoglycan 4 (PRG4) is a macromolecule distributing on the chondrocyte surface that contributes to lubrication. In this study, we investigate if PRG4 expression is associated with tribological properties of regenerated cartilage, and is able to predict its wear resistant status. : Two different strategies including bone marrow enrichment plus microfracture (B/BME-MFX) and microfracture alone (B-MFX) of cartilage repair in sheep were used. PRG4 expression and a series of tribological parameters on regenerated cartilage were rigorously examined and compared. : Highly and continuously expression of PRG4 in regenerated cartilage surface was negatively correlated with each tribological parameter (<0.0001, respectively). Multivariate analysis showed that PRG4 expression was the key predictor that contributed to the promotion of cartilage wear resistance. : Higher PRG4 expression in regenerated cartilage is significantly associated with wear resistance improvement. PRG4 may be useful for predicting the wear resistant status of regenerated cartilage and determining the optimal cartilage repair strategy.
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http://dx.doi.org/10.7150/thno.39386DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7052906PMC
February 2021

Development and validation of a 10-gene prognostic signature for acute myeloid leukaemia.

J Cell Mol Med 2020 04 9;24(8):4510-4523. Epub 2020 Mar 9.

Department of Hematology and Oncology, The Affiliated Hospital of Hangzhou Normal University, Hangzhou, China.

Acute myeloid leukaemia (AML) is the most common type of adult acute leukaemia and has a poor prognosis. Thus, optimal risk stratification is of greatest importance for reasonable choice of treatment and prognostic evaluation. For our study, a total of 1707 samples of AML patients from three public databases were divided into meta-training, meta-testing and validation sets. The meta-training set was used to build risk prediction model, and the other four data sets were employed for validation. By log-rank test and univariate COX regression analysis as well as LASSO-COX, AML patients were divided into high-risk and low-risk groups based on AML risk score (AMLRS) which was constituted by 10 survival-related genes. In meta-training, meta-testing and validation sets, the patient in the low-risk group all had a significantly longer OS (overall survival) than those in the high-risk group (P < .001), and the area under ROC curve (AUC) by time-dependent ROC was 0.5854-0.7905 for 1 year, 0.6652-0.8066 for 3 years and 0.6622-0.8034 for 5 years. Multivariate COX regression analysis indicated that AMLRS was an independent prognostic factor in four data sets. Nomogram combining the AMLRS and two clinical parameters performed well in predicting 1-year, 3-year and 5-year OS. Finally, we created a web-based prognostic model to predict the prognosis of AML patients (https://tcgi.shinyapps.io/amlrs_nomogram/).
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http://dx.doi.org/10.1111/jcmm.15109DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7176885PMC
April 2020

Influences of niobium pentoxide on roughness, hydrophilicity, surface energy and protein absorption, and cellular responses to PEEK based composites for orthopedic applications.

J Mater Chem B 2020 04;8(13):2618-2626

Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, China.

To improve the bio-performances of polyetheretherketone (PEEK) for orthopedic applications, submicro-particles of niobium pentoxide (Nb2O5) were synthesized using a sol-gel method, and PEEK/Nb2O5 composites (PNC) with a Nb2O5 content of 25v% (PNC25) and 50v% (PNC50) were fabricated by utilizing a process of pressing-sintering. The results showed that the Nb2O5 particles were not only dispersed in the composites but also exposed on the surface of the composites, which formed submicro-structural surfaces. In addition, the hydrophilicity, surface energy, surface roughness and absorption of proteins of the composites were improved with increasing Nb2O5 content. Moreover, the release of Nb ions with the highest concentration of 5.01 × 10-6 mol L-1 from the composite into the medium displayed no adverse effects on cell proliferation and morphology, indicating no cytotoxicity. Furthermore, compared with PEEK, the composites, especially PNC50, obviously stimulated adhesion and proliferation as well as osteogenic differentiation of bone mesenchymal stem cells of rats. The results suggested that the incorporation of Nb2O5 submicro-particles into PEEK produced novel bioactive composites with improved surface properties, which played important roles in regulating cell behaviors. In conclusion, the composites, especially PNC50 with good cytocompatibility and promotion of cellular responses, exhibited great potential as implantable materials for bone repair.
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http://dx.doi.org/10.1039/c9tb02456eDOI Listing
April 2020