Publications by authors named "Tingting Li"

1,180 Publications

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Intracellular Infection Decreases Milk Protein Synthesis by Preventing Amino Acid Uptake in Bovine Mammary Epithelial Cells.

Front Vet Sci 2021 16;8:756375. Epub 2021 Nov 16.

State Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot, China.

() is one of the main pathogens in cow mastitis, colonizing mammary tissues and being internalized into mammary epithelial cells, causing intracellular infection in the udder. Milk that is produced by cows that suffer from mastitis due to is associated with decreased production and changes in protein composition. However, there is limited information on how mastitis-inducing bacteria affect raw milk, particularly with regard to protein content and protein composition. The main purpose of this work was to examine how infection affects milk protein synthesis in bovine mammary epithelial cells (BMECs). BMECs were infected with , and milk protein and amino acid levels were determined by ELISA after invasion. The activity of mTORC1 signaling and the transcription factors NF-κB and STAT5 and the expression of the amino acid transporters SLC1A3 and SLC7A5 were measured by western blot or immunofluorescence and RT-qPCR. was internalized by BMECs , and the internalized bacteria underwent intracellular proliferation. Eight hours after invasion, milk proteins were downregulated, and the level of BMECs that absorbed Glu, Asp, and Leu from the culture medium and the exogenous amino acids induced β-casein synthesis declined. Further, the activity of mTORC1 signaling, NF-κB, and STAT5 was impaired, and and were downregulated. Eight hours of treatment with 100 nM rapamycin inhibited NF-κB and STAT5 activity, and expression, and milk protein synthesis in BMECs. Thus mTORC1 regulates the expression of and through NF-κB and STAT5. These findings constitute a model by which infection suppresses milk protein synthesis by decreasing amino acids uptake in BMECs.
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http://dx.doi.org/10.3389/fvets.2021.756375DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8636274PMC
November 2021

Recent Advancements in Serum Albumin-Based Nanovehicles Toward Potential Cancer Diagnosis and Therapy.

Front Chem 2021 18;9:746646. Epub 2021 Nov 18.

School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.

Recently, drug delivery vehicles based on nanotechnology have significantly attracted the attention of researchers in the field of nanomedicine since they can achieve ideal drug release and biodistribution. Among the various organic or inorganic materials that used to prepare drug delivery vehicles for effective cancer treatment, serum albumin-based nanovehicles have been widely developed and investigated due to their prominent superiorities, including good biocompatibility, high stability, nontoxicity, non-immunogenicity, easy preparation, and functionalization, allowing them to be promising candidates for cancer diagnosis and therapy. This article reviews the recent advances on the applications of serum albumin-based nanovehicles in cancer diagnosis and therapy. We first introduce the essential information of bovine serum albumin (BSA) and human serum albumin (HSA), and discuss their drug loading strategies. We then discuss the different types of serum albumin-based nanovehicles including albumin nanoparticles, surface-functionalized albumin nanoparticles, and albumin nanocomplexes. Moreover, after briefly discussing the application of serum albumin-based nanovehicles used as the nanoprobes in cancer diagnosis, we also describe the serum albumin-based nanovehicle-assisted cancer theranostics, involving gas therapy, chemodynamic therapy (CDT), phototherapy (PTT/PDT), sonodynamic therapy (SDT), and other therapies as well as cancer imaging. Numerous studies cited in our review show that serum albumin-based nanovehicles possess a great potential in cancer diagnostic and therapeutic applications.
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http://dx.doi.org/10.3389/fchem.2021.746646DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8636905PMC
November 2021

The spillover of tourism development on CO emissions: a spatial econometric analysis.

Environ Sci Pollut Res Int 2021 Dec 2. Epub 2021 Dec 2.

Graduate School of Management, Management and Science University, Shah Alam, Selangor Darul Ehsan, Malaysia.

Climate change and tourism's interaction and vulnerability have been among the most hotly debated topics recently. In this context, the study focuses on how CO emissions, the primary cause of global warming and climate change, respond to changes in tourism development. In order to do so, the impact of tourism development on CO emissions in the most visited countries is investigated. A panel data from 2000 to 2017 for top 70 tourist countries are analysed using a spatial econometric method to investigate the spatial effect of tourism on environmental pollution. The direct, indirect, and overall impact of tourism on CO emissions are estimated using the most appropriate generalized nested spatial econometric (GNS) method. The findings reveal that tourism has a positive direct effect and a negative indirect effect; both are significant at the 1% level. The negative indirect effect of tourism is greater than its direct positive effect, implying an overall significantly negative impact. Further, the outcome of financial development and CO emissions have an inverted U-shaped and U-shaped relationship in direct and indirect impacts. Population density, trade openness, and economic growth significantly influence environmental pollution. In addition, education expenditure and infrastructure play a significant moderating role among tourism and environmental pollution. The results have important policy implications as they establish an inverted-U-shaped relationship among tourism and CO emissions and indicate that while a country's emissions initially rise with the tourism industry's growth, it begins declining after a limit.
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http://dx.doi.org/10.1007/s11356-021-17026-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8638795PMC
December 2021

Maslinic Acid Attenuates Ischemia/Reperfusion Injury-Induced Myocardial Inflammation and Apoptosis by Regulating HMGB1-TLR4 Axis.

Front Cardiovasc Med 2021 10;8:768947. Epub 2021 Nov 10.

School of Medicine, Nankai University, Tianjin, China.

The inflammatory response and apoptosis are the major pathological features of myocardial ischemia/reperfusion injury (MI/RI). Maslinic acid (MA), a natural pentacyclic triterpene with various bioactivities, plays critical roles in the multiple cellular biological processes, but its protective effects on the pathophysiological processes of MI/RI have not been extensively investigated. Our study aimed to determine whether MA treatment alleviate ischemia/reperfusion (I/R)-induced myocardial inflammation and apoptosis both and , and further reveal the underlying mechanisms. An MI/RI rat model was successfully established by ligating the left anterior descending coronary artery and H9c2 cells were exposed to hypoxia/reoxygenation (H/R) to mimic I/R injury. In addition, prior to H/R stimulation or myocardial I/R operation, the H9c2 cells or rats were treated with varying concentrations of MA or vehicle for 24 h and two consecutive days, respectively. In this study, our results showed that MA could obviously increase the cell viability and decrease the cardiac enzymes release after H/R . MA could significantly improve the H/R-induced cardiomyocyte injury and I/R-induced myocardial injury in a dose-dependent manner. Moreover, MA suppressed the expression of inflammatory cytokines (tumor necrosis factor alpha [TNF-α, interleukin-1β [IL-1β and interleukin-6 [IL-6]) and the expressions of apoptosis-related proteins (cleaved caspase-3 and Bax) as well as increased the levels of anti-apoptotic protein Bcl-2 expression both and . Mechanistically, MA significantly inhibited nuclear translocation of nuclear factor-κB (NF-κB) p65 after H/R regulating high mobility group box 1 (HMGB1)/toll-like receptor 4 (TLR4) axis. Taken together, MA treatment may alleviate MI/RI by suppressing both the inflammation and apoptosis in a dose-dependent manner, and the cardioprotective effect of MA may be partly attributable to the inactivation of HMGB1/TLR4/NF-κB pathway, which offers a new therapeutic strategy for MI/RI.
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http://dx.doi.org/10.3389/fcvm.2021.768947DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8631436PMC
November 2021

Pattern Recognition Receptors (PRRs) in Macrophages Possess Prognosis and Immunotherapy Potential for Melanoma.

Front Immunol 2021 9;12:765615. Epub 2021 Nov 9.

National Key Laboratory of Medical Immunology, Institute of Immunology, Navy Medical University, Shanghai, China.

Background: Pattern recognition receptors (PRRs) family plays a vital role in the initial stage of innate immune response and the subsequent activation of adaptive immunity. Increasing evidences have indicated that several PRRs play critical roles in the progress of inflammation and tumorigenesis. However, the comprehensive significance of PRRs family in clinical prognosis of different cancers is still elusive.

Methods: We analyzed expression of 20 canonical PRRs in tumor samples from 9502 patients of 33 tumor types. Next, we used expression profiles of PRRs in skin cutaneous melanoma (SKCM) to build a Cox prognosis model. Then, we analyzed immune infiltration features and immune activity of high risk score and low risk score patients. Finally, we analyzed the single-cell sequencing data of different cancers and detected the expression of PRRs in mouse melanoma model to identify PRRs-expressing cell types.

Results: We found PRRs had a significantly positive correlation with prognosis in SKCM rather than other tumors, and PRR-based Cox model had a much better prognosis potential than any single PRR. Further analysis shows risk score could indicate immunocyte infiltration and immune activity in SKCM. We also found the expressions of some PRR genes were highly correlated with the expression of immune checkpoints molecules in SKCM, indicating they could be indicators for clinical immune therapy. Finally, we found only in SKCM samples, the expression of PRRs is especially high in a subpopulation of macrophages with a trait of CD206 low expression, probably explaining why PRRs have prognosis potential in melanoma.

Conclusions: Our study reveals PRR family in macrophages has a positive prognosis potential in melanoma and could be valuable for clinical prognosis and immune therapy.
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http://dx.doi.org/10.3389/fimmu.2021.765615DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8630683PMC
November 2021

Diagnosis experiences from 50 hepatitis B patients in Chongqing, China: a qualitative study.

BMC Public Health 2021 12 1;21(1):2195. Epub 2021 Dec 1.

School of Public Health and Management, Chongqing Medical University, No. 1 Yixueyuan Road, Yuzhong District, Chongqing, 400016, China.

Background: The aim of this study was to provide recommendations for reducing the impact of hepatitis B infection on patients with chronic hepatitis B by describing their experiences during the diagnosis process.

Methods: We conducted face-to-face interviews with 50 hepatitis B patients recruited by convenient sampling from an infectious diseases department of a teaching hospital in Chongqing, China from July to August 2019. Thematic analysis framework included interviewees' social demographic characteristics, diagnosis approach, signs and symptoms before diagnosis, feelings after diagnosis, and doctor's instructions.

Results: Most patients first detected hepatitis B through various types of physical examinations when the patients were asymptomatic or had only mild symptoms. Most patients were shocked, scared, or overwhelmed when they were diagnosed with hepatitis B. They were able to remember the doctor's instructions about maintaining a healthy lifestyle, but not impressed by the doctor's advice about regular follow-up liver function tests. The lack of regular follow-up has caused irreversible damage to some patients.

Conclusions: Most patients are passively diagnosed with hepatitis B due to their lack of awareness on active hepatitis B prevention. Patients need professional mental health care to overcome the negative emotions that following the diagnosis. Physicians' instruction should emphasize the importance of regular follow-up liver function tests in addition to a healthy lifestyle.
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http://dx.doi.org/10.1186/s12889-021-11929-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8638347PMC
December 2021

An interpretable machine learning model based on a quick pre-screening system enables accurate deterioration risk prediction for COVID-19.

Sci Rep 2021 11 30;11(1):23127. Epub 2021 Nov 30.

Department of Emergency, The First Medical Center to Chinese People's Liberation Army General Hospital, Beijing, China.

A high-performing interpretable model is proposed to predict the risk of deterioration in coronavirus disease 2019 (COVID-19) patients. The model was developed using a cohort of 3028 patients diagnosed with COVID-19 and exhibiting common clinical symptoms that were internally verified (AUC 0.8517, 95% CI 0.8433, 0.8601). A total of 15 high risk factors for deterioration and their approximate warning ranges were identified. This included prothrombin time (PT), prothrombin activity, lactate dehydrogenase, international normalized ratio, heart rate, body-mass index (BMI), D-dimer, creatine kinase, hematocrit, urine specific gravity, magnesium, globulin, activated partial thromboplastin time, lymphocyte count (L%), and platelet count. Four of these indicators (PT, heart rate, BMI, HCT) and comorbidities were selected for a streamlined combination of indicators to produce faster results. The resulting model showed good predictive performance (AUC 0.7941 95% CI 0.7926, 0.8151). A website for quick pre-screening online was also developed as part of the study.
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http://dx.doi.org/10.1038/s41598-021-02370-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8633326PMC
November 2021

[Regulatory mechanism of long noncoding RNA in the occurrence and development of leukemia: a review].

Sheng Wu Gong Cheng Xue Bao 2021 Nov;37(11):3933-3944

College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China.

Long noncoding RNAs (lncRNAs) are a class of RNA molecules that are greater than 200 nt in length and do not have protein-coding capabilities or encode micropeptides only. LncRNAs are involved in the regulation of cell proliferation, differentiation, apoptosis and other biological processes, and are closely associated with the occurrence, recurrence and metastasis of a variety of malignant hematologic diseases. This article summarizes the function, regulatory mechanism and potential clinical application of lncRNAs in leukemia. In general, lncRNAs regulate the occurrence and development of leukemia and the multi-drug resistance in chemotherapy through epigenetic modification, ribosomal RNA transcription, competitive binding with miRNA, modulating glucose metabolic pathway, and activating tumor-related signaling pathway. Studies on lncRNAs provide new references for understanding the pathogenesis of leukemia, uncovering new prognostic markers and potential therapeutic targets, and addressing the problems of drug resistance and post-treatment recurrence in patients in clinical treatment of leukemia.
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http://dx.doi.org/10.13345/j.cjb.210139DOI Listing
November 2021

African Swine Fever Virus pE199L Induces Mitochondrial-Dependent Apoptosis.

Viruses 2021 Nov 8;13(11). Epub 2021 Nov 8.

Division of Fundamental Immunology, National African Swine Fever Para-Reference Laboratory, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Harbin 150069, China.

African swine fever (ASF) is a severe hemorrhagic disease in swine characterized by massive lymphocyte depletion and cell death, with apoptosis and necrosis in infected lymphoid tissues. However, the molecular mechanism regarding ASFV-induced cell death remains largely unknown. In this study, 94 ASFV-encoded proteins were screened to determine the viral proteins involved in cell death in vitro, and pE199L showed the most significant effect. Ectopic expression of pE199L in porcine cells (CRL-2843) and human cells (HEK293T and HeLa cells) induced cell death remarkably, showing obvious shrinking, blistering, apoptotic bodies, and nuclear DNA fragments. Meanwhile, cell death was markedly alleviated when the expression of pE199L was knocked down during ASFV infection. Additionally, the expression of pE199L caused a loss of mitochondrial membrane potential, release of cytochrome C, and caspase-9 and -3/7 activation, indicating that the mitochondrial apoptotic pathway was involved in pE199L-induced apoptosis. Further investigations showed that pE199L interacted with several anti-apoptotic BCL-2 subfamily members (such as BCL-X, MCL-1, BCL-W, and BCL-2A1) and competed with BAK for BCL-X, which promoted BAK and BAX activation. Taken together, ASFV pE199L induces the mitochondrial-dependent apoptosis, which may provide clues for a comprehensive understanding of ASFV pathogenesis.
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http://dx.doi.org/10.3390/v13112240DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8617669PMC
November 2021

Quorum Quenching Enzyme (PF-1240) Capable to Degrade AHLs as a Candidate for Inhibiting Quorum Sensing in Food Spoilage Bacterium .

Foods 2021 Nov 5;10(11). Epub 2021 Nov 5.

National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, College of Food Science and Technology, Bohai University, Jinzhou 121013, China.

Quorum sensing (QS) is widely present in microorganisms in marine aquatic products. Owing to the use of antibiotics, many spoilage bacteria in aquatic products are drug resistant. In order to slow down this evolutionary trend, the inhibition of spoilage phenotype of spoilage bacteria by interfering with QS has become a research hot spot in recent years. In this study, we found a new QS quenching enzyme, PF-1240; it was cloned and expressed in 08. Sequence alignment showed that its similarity with -homoserine lactone (AHL) acylase QuiP protein of ( 0-1) was 78.4%. SDS-PAGE confirmed that the protein is a dimer composed of two subunits, which is similar to the structure of AHL acylases. The concentration of heterologous expression in (DE3) was 26.64 μg/mL. Unlike most AHL acylases, PF-1240 can quench AHLs with different carbon chain lengths and inhibit the quorum sensing of the aquatic spoilage bacterium . It can significantly reduce the formation rate of biofilm of to 44.4% and the yield of siderophores to 54%, inhibit the production of protease and lipase, and interfere with the motility of . Through these corruption phenotypes, the specific application effect of PF-1240 can be further determined to provide a theoretical basis for its application in the preservation of practical aquatic products.
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http://dx.doi.org/10.3390/foods10112700DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8622684PMC
November 2021

T Cell Immunity Evaluation and Immunodominant Epitope T Cell Receptor Identification of Severe Acute Respiratory Syndrome Coronavirus 2 Spike Glycoprotein in COVID-19 Convalescent Patients.

Front Cell Dev Biol 2021 3;9:696662. Epub 2021 Nov 3.

Department of Immunology, College of Basic Medicine, Chongqing Medical University, Chongqing, China.

A better understanding of the role of T cells in the immune response to Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is helpful not only for vaccine development but also for the treatment of COVID-19 patients. In this study, we determined the existence of SARS-CoV-2-specific T cells in the blood of COVID-19 convalescents. Meanwhile, the specific T cell response in the non-RBD region was stronger than in the RBD region. We also found that SARS-CoV-2 S-specific reactive CD4 T cells exhibited higher frequency than CD8 T cells in recovered COVID-19 patients, with greater number of corresponding epitopes presented. Importantly, we isolated the SARS-CoV-2-specific CD4 T cell receptors (TCRs) and inserted the TCRs into allogenic CD4 T cells. These TCR-T cells can be activated by SARS-CoV-2 spike peptide and produce IFN-γ . These results might provide valuable information for the development of vaccines and new therapies against COVID-19.
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http://dx.doi.org/10.3389/fcell.2021.696662DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8595245PMC
November 2021

Antimicrobial compounds from an FDA-approved drug library with activity against Streptococcus suis.

J Appl Microbiol 2021 Nov 20. Epub 2021 Nov 20.

State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.

Aim: Antimicrobial resistance (AMR) has become a global concern. Developing novel antimicrobials is one of the most effective approaches in tackling AMR. Considering its relatively low cost and risk, drug repurposing has been proposed as a valuable approach for novel antimicrobial discovery. The aim of this study was to screen for antimicrobial compounds against Streptococcus suis, an important zoonotic bacterial pathogen, from an Food and Drug Administration (FDA)-approved drug library.

Methods And Results: In this study, we tested the antimicrobial activity of 1815 FDA-approved drugs against S. suis. Sixty-seven hits were obtained that showed a growth inhibition of more than 98%. After excluding already known antibiotics and antiseptics, 12 compounds were subjected to minimal inhibition concentration (MIC) assessment against S. suis. This showed that pralatrexate, daunorubicin (hydrochloride), teniposide, aclacinomycin A hydrochloride and floxuridine gave a relatively low MIC, ranging from 0.85 to 5.25 μg/ml. Apart from pralatrexate, the remaining four drugs could also inhibit the growth of antimicrobial-resistant S. suis. It was also demonstrated that these four drugs had better efficacy against Gram-positive bacteria than Gram-negative bacteria. Cytotoxicity assays showed that floxuridine and teniposide had a relatively high 50% cytotoxic concentration (CC ). The in vivo efficacy of floxuridine was analysed using a Galleria mellonella larvae infection model, and the results showed that floxuridine was effective in treating S. suis infection in vivo.

Conclusions: Five compounds from the FDA-approved drug library showed high antimicrobial activity against S. suis, among which floxuridine displayed potent in vivo efficacy that is worth further development.

Significance And Impact Of Study: Our study identified several antimicrobial compounds that are effective against S. suis, which provides a valuable starting point for further antimicrobial development.
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http://dx.doi.org/10.1111/jam.15377DOI Listing
November 2021

Screening and validation of quorum quenching enzyme PF2571 from Pseudomonas fluorescens strain PF08 to inhibit the spoilage of red sea bream filets.

Int J Food Microbiol 2021 Nov 14;362:109476. Epub 2021 Nov 14.

College of Food Science and Technology, Bohai University, Jinzhou, Liaoning 121013, China; National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Jinzhou, Liaoning 121013, China.

Bacteria are the main cause of spoilage for fish and fishery products. Through the inactivation of the quorum sensing (QS) system, quorum quenching (QQ) enzymes can block the synthesis of bacterial virulence factors and effectively inhibit bacteria-induced food spoilage. This study analyzed the changes of microbiota in red sea bream filets during refrigerated storage. The results showed a decrease in microbial diversity with storage time, with Aeromonas veronii becoming the dominant bacteria on day 4. A novel N-acyl homoserine lactones (AHL) acylase PF2571, from the screened QQ bacterium Pseudomonas fluorescens PF08, was identified and expressed in Escherichia coli to evaluate its QQ efficiency and effects on spoilage potential. Spoilage-related QS factors of A. veronii BY-8, including biofilm formation, motility, and protease, lipase, and alginate production, were inhibited by PF2571. Its inhibitory effect on red sea bream spoilage was demonstrated by the lower freshness indicators for PF2571 treated filets. Our study demonstrates the potential of the QQ enzyme for prolonging the shelf life of fish and fishery products.
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http://dx.doi.org/10.1016/j.ijfoodmicro.2021.109476DOI Listing
November 2021

Characteristics of ocular findings of patients with neuronal intranuclear inclusion disease.

Neurol Sci 2021 Nov 19. Epub 2021 Nov 19.

Department of Ophthalmology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Xuhui District, 600 Yishan Road, Shanghai, 200233, China.

Purpose: This study aimed to explore the ocular characteristics of neuronal intranuclear inclusion disease (NIID), caused by GGC repeat expansion in the NOTCH2NLC gene, combined with the systemic clinical manifestations, and propose early diagnostic features of NIID.

Methods: Six patients (12 eyes) were enrolled in this study. In vivo corneal confocal microscopy (IVCCM), fundus photography, fundus autofluorescence (FAF) imaging, optical coherence tomography (OCT), full-field electroretinography (ERG), and electromyography were performed.

Results: The average corneal nerve fiber density (CNFD) was 6.83 ± 4.96 number/mm, and the corneal nerve fiber length (CNFL) was 6.76 ± 1.96 mm/mm. The nerves were looser and more curved in affected individuals. Dendritic cells were observed in patients with NIID. Chorioretinal atrophy, hyper-AF spots, and outer retinal abnormalities were observed during FAF imaging and OCT examinations. In full-field ERGs, the amplitudes of the a-wave and b-wave reduced or extinguished over time. The compound muscle action potential and motor nerve conduction velocity of the left common peroneal nerve decreased substantially.

Conclusion: The findings of IVCCM and retinal changes should be included in the diagnostic criteria for NIID. Corneal confocal characteristics may precede the systemic neurological manifestations and provide a clinical basis for the early treatment and staging of the disease. ClincalTrials.gov. Identifier: ChiCTR21000500227.
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http://dx.doi.org/10.1007/s10072-021-05748-4DOI Listing
November 2021

Efficacy and safety of targeted drugs in advanced or metastatic gastric and gastroesophageal junction cancer: A network meta-analysis.

J Clin Pharm Ther 2021 Nov 18. Epub 2021 Nov 18.

Provincial-Level Key Laboratory of Molecular Medicine of Major Diseases and Study on Prevention and Treatment of Traditional Chinese Medicine, Gansu University of Chinese Medicine, Lanzhou, Gansu, China.

What Is Known And Objective: An increasing number of targeted drugs have been used to treat advanced or metastatic gastric cancer (GC) and gastroesophageal junction cancer (GEJC). However, the optimal treatment efficacy of these drugs is still controversial. The aims of this study are to systematically summarize the efficacy and safety of current targeted drugs for advanced or metastatic GC and GEJC.

Methods: PubMed, EmBase, Cochrane Library, Web of Science and ClinicalTrials were searched for double-blind randomized controlled trials (RCTs) on GC and GEJC up to December 2019. Additionally, we updated the literature search from Jan, 1, 2020 to September 30, 2021. Narrative and quantitative analysis were performed to analyse the efficacy and safety. STATA 15.1 was used to identify publication bias, and the SUCRA (surface under the cumulative ranking) curve was conducted to rank the treatments for each outcome.

Results: A total of 27 RCTs with 9295 GC and GEJC patients treated by 19 drugs were included. SUCRA showed that regorafenib was the most likely to improve patients' progression-free survival (96.4%), followed by apatinib (90.7%), nivolumab (82.4%), everolimus (76.5%) and pertuzumab (68.5%). Meanwhile, apatinib (92.4%) was most likely to improve overall survival, followed by nivolumab (87.9%), regorafenib (72.5%), olaparib (67.7%) and lapatinib (63.2%). Additionally, neutropenia, diarrhoea and fatigue were the most common adverse events caused by these drugs, followed by pain, nausea, decreased appetite, anaemia and vomiting.

What Is New And Conclusion: Regorafenib and nivolumab have higher efficacy and tolerability and are the most advantageous for advanced GC and GEJC. Moreover, apatinib has higher efficacy but lower tolerability. Everolimus and pertuzumab combined with chemotherapy have best secondary higher efficacy for progression-free survival and good tolerability. Lapatinib and olaparib combined with chemotherapy have moderate efficacy for overall survival and good tolerability.
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http://dx.doi.org/10.1111/jcpt.13570DOI Listing
November 2021

A metabolite panel that differentiates Alzheimer's disease from other dementia types.

Alzheimers Dement 2021 Nov 17. Epub 2021 Nov 17.

Innovation Center for Neurological Disorders and Department of Neurology, Xuanwu Hospital, Capital Medical University, National Clinical Research Center for Geriatric Diseases, Beijing, China.

Introduction: Alzheimer's disease (AD) is associated with altered metabolites. This study aimed to determine the validity of using circulating metabolites to differentiate AD from other dementias.

Methods: Blood metabolites were measured in three data sets. Data set 1 (controls, 27; AD, 28) was used for analyzing differential metabolites. Data set 2 (controls, 93; AD, 92) was used to establish a diagnostic AD model with use of a metabolite panel. The model was applied to Data set 3 (controls, 76; AD, 76; other dementias, 205) to verify its capacity for differentiating AD from other dementias.

Results: Data set 1 revealed 7 upregulated and 77 downregulated metabolites. In Data set 2, a panel of 11 metabolites was included in a model that could distinguish AD from controls. In Data set 3, this panel was used to successfully differentiate AD from other dementias.

Discussion: This study revealed an AD-specific panel of 11 metabolites that may be used for AD diagnosis.
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http://dx.doi.org/10.1002/alz.12484DOI Listing
November 2021

Effects of silkworm excrement and water management on the accumulation of Cd and As in different varieties of rice and an assessment of their health risk.

Ecotoxicol Environ Saf 2021 Nov 13;228:112974. Epub 2021 Nov 13.

Agricultural Resources and Environment Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China. Electronic address:

Rice (Oryza sativa L.) consumption represents a major route for the exposure to cadmium (Cd) and arsenic (As) in many countries. Two varieties of rice that were grown in soils contaminated with Cd and As were evaluated for the accumulation of these toxins in rice grains and the risks of exposure of local residents to Cd and As when treated with different amounts of silkworm excrement and types of water management. Silkworm excrement, water management and the variety of rice significantly affected the accumulation of Cd and As in rice. The combination of multiple measures can be more effective at reducing heavy metals than the use of single measure, i.e., silkworm excrement management, water management, and the selection of low accumulation variety. The use of a variety that accumulates low amounts of Cd combined with 1% silkworm excrement management can effectively increase the soil pH and electrical conductivity (EC) and decrease the contents of soil available Cd and the transfer coefficients of Cd in rice, subsequently reducing the concentrations of Cd in rice grains and lowering the health risks of the intake of Cd. Similarly, the use of a conventional rice variety combined with alternating periods of drying and wetting in the three weeks before and after the heading stage decreased the contents of soil available As and the transfer coefficient of As in rice, subsequently reducing the accumulation of As in the grains and lowering the health risk of the intake of As. The significantly lower concentrations of Cd and As in rice grains and the risk of intake of Cd and As from rice was observed using a conventional rice variety combined with alternating drying-wetting in the three weeks before and after the heading stage and 1% silkworm excrement management. Thus, the combination of multiple measures in the coexistence of Cd and As in contaminated soils can be a promising strategy to avoid serious health risks and ensure the safety of food for local residents.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112974DOI Listing
November 2021

Net CO and CH emissions from restored mangrove wetland: New insights based on a case study in estuary of the Pearl River, China.

Sci Total Environ 2021 Nov 12:151619. Epub 2021 Nov 12.

Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519000, China; School of Atmospheric Sciences, Guangdong Province Key Laboratory for Climate Change and Natural Disaster Studies, Sun Yat-sen University, Zhuhai 519000, China.

Mangroves have the potential to affect climate via C sequestration and methane (CH) emissions. With half of the world's mangroves lost during the 20th century, mangrove restoration in mitigating greenhouse gases has been increasingly recognized. However, the carbon exchanges during restored processes still remain large uncertain. In this study, we analyzed the temporal variations of CO and CH fluxes and their environmental controls during 2019 and 2020 based on a closed-path eddy covariance (EC) system in a 12-year restored subtropical mangrove wetland, in estuary of the Pearl River, southeastern China. We also estimated the CO and CH fluxes and their climate effect from the beginning of restoration by Random Forest algorithm (RF). The EC observations showed that annually the 12-year restored mangrove acted as CO and CH sources, with net CO ecosystem exchange (NEE) of 82-175 gC·m·a and CH fluxes of 24.7-26.3 gC·m·a. Low vegetation gross primary productivity (GPP) and high ecosystem respiration (Re) caused net CO emissions in the mangroves. The estimation by RF indicated that the mangroves were always a CO source after the beginning of restoration, but the annual NEE was linearly decreased from 233 to 131 gC·m·a from 2008 to 2020. The annual CH emissions continually increased from 19.0 to 25.8 gC·m·a after restoration. As a result, the restored mangrove had a positive effect on climate warming, with increased GWP from 1276 to 1386 g CO-eq ·m·a from 2008 to 2020. This is mainly due to lower GPP and higher Re by young restored mangroves, large water area as well as low salinity induced strong CH emissions. Our results indicate new sights that young restored mangrove with large area of water surface may act as carbon sources. However, the long-term climate and ecosystem benefits due to mangrove restoration should not be ignored in future.
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http://dx.doi.org/10.1016/j.scitotenv.2021.151619DOI Listing
November 2021

The stress granule protein G3BP1 promotes pre-condensation of cGAS to allow rapid responses to DNA.

EMBO Rep 2021 Nov 15:e53166. Epub 2021 Nov 15.

State Key Laboratory of Proteomics, National Center of Biomedical Analysis, Beijing, China.

Cyclic GMP-AMP synthase (cGAS) functions as a key sensor for microbial invasion and cellular damage by detecting emerging cytosolic DNA. Here, we report that GTPase-activating protein-(SH3 domain)-binding protein 1 (G3BP1) primes cGAS for its prompt activation by engaging cGAS in a primary liquid-phase condensation state. Using high-resolution microscopy, we show that in resting cells, cGAS exhibits particle-like morphological characteristics, which are markedly weakened when G3BP1 is deleted. Upon DNA challenge, the pre-condensed cGAS undergoes liquid-liquid phase separation (LLPS) more efficiently. Importantly, G3BP1 deficiency or its inhibition dramatically diminishes DNA-induced LLPS and the subsequent activation of cGAS. Interestingly, RNA, previously reported to form condensates with cGAS, does not activate cGAS. Accordingly, we find that DNA - but not RNA - treatment leads to the dissociation of G3BP1 from cGAS. Taken together, our study shows that the primary condensation state of cGAS is critical for its rapid response to DNA.
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http://dx.doi.org/10.15252/embr.202153166DOI Listing
November 2021

Prediction of P-tau/Aβ42 in the cerebrospinal fluid with blood microRNAs in Alzheimer's disease.

BMC Med 2021 11 15;19(1):264. Epub 2021 Nov 15.

Innovation Center for Neurological Disorders and Department of Neurology, Xuanwu Hospital, Capital Medical University, National Clinical Research Center for Geriatric Diseases, 45 Changchun St, Beijing, China.

Background: The most common biomarkers of Alzheimer's disease (AD) are amyloid β (Aβ) and tau, detected in cerebrospinal fluid (CSF) or with positron emission tomography imaging. However, these procedures are invasive and expensive, which hamper their availability to the general population. Here, we report a panel of microRNAs (miRNAs) in serum that can predict P-tau/Aβ42 in CSF and readily differentiate AD from other dementias, including vascular dementia (VaD), Parkinson disease dementia (PDD), behavioral variant frontotemporal dementia (bvFTD), and dementia with Lewy body (DLB).

Methods: RNA samples were extracted from the participant's blood. P-tau/Aβ42 of CSF was examined for diagnostic purposes. A pilot study (controls, 21; AD, 23), followed by second (controls, 216; AD, 190) and third groups (controls, 153; AD, 151), is used to establish and verify a predictive model of P-tau/Aβ42 in CSF. The test is then applied to a fourth group of patients with different dementias (controls, 139; AD,155; amnestic mild cognitive impairment [aMCI], 55; VaD, 51; PDD, 53; bvFTD, 53; DLB, 52) to assess its diagnostic capacity.

Results: In the pilot study, 29 upregulated and 31 downregulated miRNAs in the AD group were found. In Dataset 2, these miRNAs were then included as independent variables in the linear regression model. A seven-microRNA panel (miR-139-3p, miR-143-3p, miR-146a-5p, miR-485-5p, miR-10a-5P, miR-26b-5p, and miR-451a-5p) accurately predicted values of P-tau/Aβ42 of CSF. In Datasets 3 and 4, by applying the predicted P-tau/Aβ42, the predictive model successfully differentiates AD from controls and VaD, PDD, bvFTD, and DLB.

Conclusions: This study suggests that the panel of microRNAs is a promising substitute for traditional measurement of P-tau/Aβ42 in CSF as an effective biomarker of AD.
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http://dx.doi.org/10.1186/s12916-021-02142-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8591889PMC
November 2021

Cellular retinol binding protein-1 inhibits cancer stemness via upregulating WIF1 to suppress Wnt/β-catenin pathway in hepatocellular carcinoma.

BMC Cancer 2021 Nov 14;21(1):1224. Epub 2021 Nov 14.

Jiangsu Key Laboratory of Immunity and Metabolism, Department of Pathogenic Biology and Immunology, Xuzhou Medical University, Xuzhou, Jiangsu Province, 221004, People's Republic of China.

Background: CRBP-1, a cytosolic chaperone of vitamin A, is identified in a serious number of cancers; however, its biological role in hepatocellular carcinoma (HCC) needs to be further explored. The aim of our present study is to explore the roles and mechanisms of CRBP-1 in regulating liver cancer by using in vitro and in vivo biology approaches.

Methods: The expression level of CRBP-1 was detected using immunohistochemistry in HCC and matching adjacent non-tumorous liver tissues. Following established stable CRBP-1 overexpressed HCC cell lines, the cell growth and tumorigenicity were investigated both in vitro and in vivo. Intracellular retinoic acid was quantified by ELISA. The relationship between CRBP-1 and WIF1 was validated by using dual luciferase and ChIP analyses.

Results: The low expression of CRBP-1 was observed in HCC tissues compared to the normal liver tissues, while high CRBP-1 expression correlated with clinicopathological characteristics and increased overall survival in HCC patients. Overexpression of CRBP-1 significantly inhibited cell growth and tumorigenicity both in vitro and in vivo. Moreover, overexpression of CRBP-1 suppressed tumorsphere formation and cancer stemness related genes expression in HCC. Mechanically, CRBP-1 inhibited Wnt/β-catenin signaling pathway to suppress cancer cell stemness of HCC. Furthermore, our results revealed that CRBP-1 could increase the intracellular levels of retinoic acid, which induced the activation of RARs/RXRs leading to the transcriptional expression of WIF1, a secreted antagonist of the Wnt/β-catenin signaling pathway, by physically interacting with the region on WIF1 promoter.

Conclusion: Our findings reveal that CRBP-1 is a crucial player in the initiation and progression of HCC, which provide a novel independent prognostic biomarker and therapeutic target for the diagnosis and treatment of HCC.
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http://dx.doi.org/10.1186/s12885-021-08967-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8590789PMC
November 2021

Did patients with COVID-19 receive timely treatment in the early epidemic?-A systematic review and meta-analysis.

Ann Palliat Med 2021 Oct 25. Epub 2021 Oct 25.

School of Public Health, Chengdu Medical College, Chengdu, China.

Background: Corona virus disease 2019 (COVID-19) showed a significant difference in case fatality rate between different regions at the early stage of the epidemic. In addition to the well-known factors such as age structure, detection efficiency, and race, there was also a possibility that medical resource shortage caused the increase of the case fatality rate in some regions.

Methods: Medline, Cochrane Library, Embase, Web of Science, CBM, CNKI, and Wan fang of identified articles were searched through 29 June 2020. Cohort studies and case series with duration information on COVID-19 patients were included. Two independent reviewers extracted the data using a standardized data collection form and assessed the risk of bias. Data were synthesized through description and analysis methods including a meta-analysis.

Results: A total of 109 articles were retrieved. The time interval from onset to the first medical visit of COVID-19 patients in China was 3.38±1.55 days (corresponding intervals in Hubei province, non-Hubei provinces, Wuhan, Hubei provinces without Wuhan were 4.22±1.13, 3.10±1.57, 4.20±0.97, and 4.34±1.72 days, respectively). The time interval from onset to the hospitalization of COVID-19 patients in China was 8.35±6.83 days (same corresponding intervals were 12.94±7.43, 4.17±1.45, 14.86±7.12, and 5.36±1.19 days, respectively), and when it was outside China, this interval was 5.27±1.19 days.

Discussion: In the early stage of the COVID-19 epidemic, patients with COVID-19 did not receive timely treatment, resulting in a higher case fatality rate in Hubei province, partly due to the relatively insufficient and unequal medical resources. This research suggested that additional deaths caused by the out-of-control epidemic can be avoided if prevention and control work is carried out at the early stage of the epidemic.

Trial Registration: CRD42020195606.
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http://dx.doi.org/10.21037/apm-21-1975DOI Listing
October 2021

R2R3-MYB Transcription Factor Confers Drought Tolerance in Herbaceous Peony ( Pall.).

Int J Mol Sci 2021 Nov 2;22(21). Epub 2021 Nov 2.

Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, China.

The MYB transcription factor (TF) is crucial for plant growth, development, and response to abiotic stress, but it is rarely reported in the herbaceous peony ( Pall.). Here, an MYB TF gene was isolated, and based on our prior mRNA data from samples, it was treated with drought stress (DS). Its complete cDNA structure was 1314 bp, which encoded 291 amino acids (aa). Furthermore, using sequence alignment analysis, we demonstrated that was an R2R3-MYB TF. We also revealed that PlMYB108 was primarily localized in the nucleus. Its levels rose during DS, and it was positively correlated with drought tolerance (DT) in . In addition, when was overexpressed in tobacco plants, the flavonoid content, antioxidant enzyme activities, and photosynthesis were markedly elevated. Hence, the transgenic plants had stronger DT with a higher leaf water content and lower HO accumulation compared to the wild-type (WT) plants. Based on these results, is a vital gene that serves to increase flavonoid accumulation, reactive oxygen species (ROS), scavenging capacity, and photosynthesis to confer DT. The results would provide a genetic resource for molecular breeding to enhance plant DT.
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http://dx.doi.org/10.3390/ijms222111884DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8584830PMC
November 2021

Label-Free Quantitative Proteome Analysis Reveals the Underlying Mechanisms of Grain Nuclear Proteins Involved in Wheat Water-Deficit Response.

Front Plant Sci 2021 25;12:748487. Epub 2021 Oct 25.

Beijing Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, College of Life Sciences, Capital Normal University, Beijing, China.

In this study, we performed the first nuclear proteome analysis of wheat developing grains under water deficit by using a label-free based quantitative proteomic approach. In total, we identified 625 unique proteins as differentially accumulated proteins (DAPs), of which 398 DAPs were predicted to be localized in nucleus. Under water deficit, 146 DAPs were up-regulated and mainly involved in the stress response and oxidation-reduction process, while 252 were down-regulated and mainly participated in translation, the cellular amino metabolic process, and the oxidation-reduction process. The -acting elements analysis of the key nuclear DAPs encoding genes demonstrated that most of these genes contained the same -acting elements in the promoter region, mainly including ABRE involved in abscisic acid response, antioxidant response element, MYB responsive to drought regulation and MYC responsive to early drought. The -acting elements related to environmental stress and hormones response were relatively abundant. The transcription expression profiling of the nuclear up-regulated DAPs encoding genes under different organs, developmental stages and abiotic stresses was further detected by RNA-seq and Real-time quantitative polymerase chain reaction, and more than 50% of these genes showed consistency between transcription and translation expression. Finally, we proposed a putative synergistic responsive network of wheat nuclear proteome to water deficit, revealing the underlying mechanisms of wheat grain nuclear proteome in response to water deficit.
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http://dx.doi.org/10.3389/fpls.2021.748487DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8572964PMC
October 2021

Factors affecting the electron-phonon coupling in FeSe under pressure.

Phys Chem Chem Phys 2021 Nov 17;23(44):25107-25113. Epub 2021 Nov 17.

Key Laboratory of Materials Physics, Institute of Solid State Physics, HFIPS, Chinese Academy of Sciences, Hefei 230031, China.

Hydrostatic pressure on superconductor FeSe increases the Wyckoff position, , and decreases lattice constants. However, previously only the increasing of was emphasized to be important in determining the electron-phonon coupling. We explicitly study each of the two factors individually to understand its influence on the electron-phonon coupling, band structure and Raman frequencies. We find that the increasing of enhances the states around the Fermi level more, while the decreasing of the lattice constants enhances the phonon frequencies more, which together increase the electron-phonon coupling under pressure. Based on the above facts, we predict and prove that the in-plane biaxial strain on FeSe increases the electron-phonon coupling due to the increasing value and the decreasing in-plane lattice constant. Our results uncover the factors affecting the increase of the electron-phonon coupling and provide information on how to enhance the electron-phonon coupling in FeSe.
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http://dx.doi.org/10.1039/d1cp02749bDOI Listing
November 2021

Effects of rocuronium dosage on intraoperative neurophysiological monitoring in patients undergoing spinal surgery.

J Clin Pharm Ther 2021 Nov 8. Epub 2021 Nov 8.

Department of Anesthesiology, Second Affiliated Hospital of Harbin Medical University, Harbin, China.

What Is Known And Objective: Intraoperative neurophysiological monitoring (IONM) has been widely used in clinical practice. Therefore, the influence of neuromuscular blockers essential for spinal anaesthesia on IONM is worthy of our attention, but no randomized study has evaluated the dose-response effect. This study investigated the effects of different doses of rocuronium bromide on the intraoperative monitoring of motor evoked potentials (MEPs).

Methods: We conducted a randomized, double-blind trial to assess the effects of three rocuronium bromide doses (6.0, 9.0, 12 μg·kg ·min ) combined with intravenous infusion of propofol 6-8 mg·kg ·h and remifentanil 10 μg·kg ·h on the amplitudes of somatosensory evoked potentials (SEPs) and MEPs at the time of the baseline recording (T ), before pedicle screw placement (T ) and before spinal canal decompression (T ). Secondary outcomes included measurement of neuromuscular function, the occurrence of unexpected intraoperative body movement and recovery of spontaneous breathing.

Results And Discussion: A total of 123 patients were enrolled, and 120 patients were ultimately analysed. No differences were observed in the amplitude of SEPs among the three groups (p > 0.05). The MEP amplitude differences at T , T and T in all limbs did not differ in patients receiving rocuronium at 6.0 μg·kg ·min and 9.0 μg·kg ·min (p > 0.05). However, when rocuronium was administered at 12.0 μg·kg ·min , MEP amplitudes at the time point T were significantly attenuated compared with the time points T and T in both right upper limb and left lower limb (p = 0.002, p = 0.025, respectively). In patients treated with rocuronium 6.0 μg·kg ·min , the incidence of unexpected body movement was significantly higher (p = 0.026), and the train-of-four count (TOF count) showed a significant increase at T and T (p < 0.001) compared to other doses.

What Is New And Conclusion: Rocuronium bromide at a rate of 9.0 μg·kg ·min provided suitable and adequate muscle relaxation without inhibiting IONM; thus, this dose is recommended for spinal surgery.
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http://dx.doi.org/10.1111/jcpt.13557DOI Listing
November 2021

Sustainable Fenton-like degradation of methylene blue over MnO-loaded poly(amidoxime-hydroxamic acid) cellulose microrods.

Int J Biol Macromol 2021 Nov 6. Epub 2021 Nov 6.

College of Light-Textile Engineering and Art, Anhui Agricultural University, Hefei, Anhui 230036, China.

Catalysts based on cellulose/metal oxide hybrids are considered effective for the remediation of dye wastewater. However, the difficult recovery of commonly used nanocellulose and the weak binding strength of metal oxide nanoparticles restrict their wide application. Herein, MnO nanoparticle-loaded poly(amidoxime-hydroxamic acid) modified microcrystalline cellulose ([email protected]) catalysts were synthesized via an oximation reaction followed by in-situ growth. Morphology, crystallinity and textural characteristics of pAHA-MCC before and after deposition of MnO nanoparticles were characterized by SEM, EDS, FTIR, XRD and XPS analyses. The main results indicated the formation of hierarchical porous structured cellulose microrods with uniform distribution of hydrangea flower-like MnO nanoparticles. In the presence of HO, [email protected] displayed good catalytic performance toward the degradation of methylene blue (MB) over a wide pH range of 3-10, due to the advanced Fenton-like catalysis. Reaction conditions, such as amount of HO used, the initial MB concentration and catalyst dosage were also investigated. The optimized system showed 97.6% removal of MB in 25 min for 100 mg/L MB solution, with very little decrease in performance after 5 cycles. This work provides a facile and promising strategy for the development of biodegradable and sustainable architectures capable of efficiently degrading dye wastewater.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.11.026DOI Listing
November 2021

Construction and Validation of a Predictive Nomogram Based on Ultrasound for Lymph Node Metastasis of Papillary Thyroid Carcinoma in the Cervical Central Region.

Ultrasound Q 2021 Nov 5. Epub 2021 Nov 5.

Department of Ultrasound, Zhangzhou Hospital affiliated to Fujian Medical University, Zhangzhou Department of Clinical Medicine, Quanzhou Medical College, Quanzhou, Fujian, China.

Abstract: To establish and validate a nomogram for predicting lymph node metastasis (LNM) of papillary thyroid carcinoma (PTC) in the cervical central region. This retrospective study included 287 PTC patients with 309 nodules treated from December 2018 to May 2020 at our hospital. The cohort was divided randomly into a training set and a testing set according to a 7:3 ratio. The training set contained 216 nodules, and the testing set contained 93 nodules. The nomogram was developed using the training set, and the data of the testing set were used to validate the performance of nomogram. The predictive accuracy and discriminative ability of the nomogram were determined by concordance index (C-index) and calibration curve. The study showed multifocality, thyroid lesion size, and American College of Radiology Thyroid Imaging, Reporting and Data System (TI-RADS) score were significantly independently associated with LNM in the cervical central region. In the testing set, the calibration curve showed that the nomogram had good discrimination with a C-index of 0.775 (95% confidence interval, 0.680-0.869) and adequate calibration (P = 0.808). By decision curve analysis and clinical impact curve analysis, the nomogram was shown to have a satisfactory net benefit between thresholds of 0.40 and 0.75. The nomogram can be used for predicting LNM of PTC in the cervical central region and may provide valuable guidance for planning the surgical treatment of PTC patients.
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http://dx.doi.org/10.1097/RUQ.0000000000000583DOI Listing
November 2021

Potent SARS-CoV-2 neutralizing antibodies with protective efficacy against newly emerged mutational variants.

Nat Commun 2021 11 2;12(1):6304. Epub 2021 Nov 2.

Key Laboratory of Medical Molecular Virology, Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, 200032, China.

Accumulating mutations in the SARS-CoV-2 Spike (S) protein can increase the possibility of immune escape, challenging the present COVID-19 prophylaxis and clinical interventions. Here, 3 receptor binding domain (RBD) specific monoclonal antibodies (mAbs), 58G6, 510A5 and 13G9, with high neutralizing potency blocking authentic SARS-CoV-2 virus display remarkable efficacy against authentic B.1.351 virus. Surprisingly, structural analysis has revealed that 58G6 and 13G9 both recognize the steric region S on the RBD, overlapping the E484K mutation presented in B.1.351. Also, 58G6 directly binds to another region S in the RBD. Significantly, 58G6 and 510A5 both demonstrate prophylactic efficacy against authentic SARS-CoV-2 and B.1.351 viruses in the transgenic mice expressing human ACE2 (hACE2), protecting weight loss and reducing virus loads. Together, we have evidenced 2 potent neutralizing Abs with unique mechanism targeting authentic SARS-CoV-2 mutants, which can be promising candidates to fulfill the urgent needs for the prolonged COVID-19 pandemic.
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http://dx.doi.org/10.1038/s41467-021-26539-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8563728PMC
November 2021

Associations between screen time, physical activity, and depressive symptoms during the 2019 coronavirus disease (COVID-19) outbreak among Chinese college students.

Environ Health Prev Med 2021 Nov 2;26(1):107. Epub 2021 Nov 2.

Department of Maternal, Child and Adolescent Health, School of Public Health, Anhui Medical University, No. 81 Meishan Road, Hefei, 230032, Anhui, China.

Background: The 2019 novel coronavirus disease (COVID-19) emerges in China, which spreads rapidly and becomes a public health emergency of international concern. Chinese government has promptly taken quarantine measures to block the transmission of the COVID-19, which may cause deleterious consequences on everyone's behaviors and psychological health. Few studies have examined the associations between behavioral and mental health in different endemic areas. This study aimed to describe screen time (ST), physical activity (PA), and depressive symptoms, as well as their associations among Chinese college students according to different epidemic areas.

Methods: The study design is cross-sectional using online survey, from 4 to 12 February 2020, 14,789 college students accomplished this online study, participants who did not complete the questionnaire were excluded, and finally this study included 11,787 college students from China.

Results: The average age of participants was 20.51 ± 1.88 years. 57.1% of the college students were male. In total, 25.9% of college students reported depression symptoms. ST > 4 h/day was positively correlated with depressive symptoms (β = 0.48, 95%CI 0.37-0.59). COVID-19ST > 1 h/day was positively correlated with depressive symptoms (β = 0.54, 95%CI 0.43-0.65), compared with COVID-19ST ≤ 0.5 h/day. Compared with PA ≥ 3 day/week, PA < 3 day/week was positively associated with depression symptoms (β = 0.01, 95%CI 0.008-0.012). Compared with low ST and high PA, there was an interaction association between high ST and low PA on depression (β = 0.31, 95%CI 0.26-0.36). Compared with low COVID-19ST and high PA, there was an interaction association between high COVID-19ST and low PA on depression (β = 0.37, 95%CI 0.32-0.43). There were also current residence areas differences.

Conclusions: Our findings identified that high ST or low PA was positively associated with depressive symptoms independently, and there was also an interactive effect between ST and PA on depressive symptoms.
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http://dx.doi.org/10.1186/s12199-021-01025-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8562930PMC
November 2021
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