Publications by authors named "Tingting Jiang"

213 Publications

LncRNA GAS8-AS1 downregulates lncRNA NEAT1 to inhibit glioblastoma cell proliferation.

Brain Behav 2021 May 4:e02128. Epub 2021 May 4.

Department of Neurology, People's Hospital of Deyang City, Deyang, China.

Background: LncRNA GAS8-AS1 has been reported to participate in several types of cancer, while its role in glioblastoma (GBM) is unknown. In the present study, we aimed to investigate the function of GAS8-AS1 in GBM and the underlying mechanisms.

Methods: The expression levels of GAS8-AS1 and NEAT1 in GBM patients and the healthy controls were measured by performing RT-qPCR. Diagnostic values of plasma GAS8-AS1 and NEAT1 for GBM were analyzed by performing ROC curve analysis with GBM patients as true positive cases and the healthy controls as true negative cases. Linear regression analysis was performed to study the correlation between the expression levels of GAS8-AS1 and NEAT1. The expression levels of GAS8-AS1 and NEAT1 in GBM cells were also determined by RT-qPCR. CCK-8 and transwell invasion assays were performed to detect the proliferation and invasion of GBM cells. Western blot assay was performed to detect the expression levels of β-catenin, Axin2, c-myc, cyclin D1, and GAPDH in GBM cells.

Results: GAS8-AS1 was downregulated, while lncRNA NEAT1 was upregulated in the plasma of GBM patients. Altered expression levels of GAS8-AS1 and NEAT1 distinguished GBM patients from the healthy controls. The expression of GAS8-AS1 and NEAT1 was inversely correlated only in GBM patients. Overexpression of GAS8-AS1 reduced the expression levels of NEAT1 in GBM cells, while knock-down of GAS8-AS1 increased the expression levels of NEAT1. However, overexpression of NEAT1 showed no significant effects on the expression of GAS8-AS1. Knock-down of GAS8-AS1 promoted GBM cell proliferation and invasion and enhanced the activation of the Wnt/β-catenin pathway. However, the effects of knock-down of GAS8-AS1 were alleviated by the knock-down of NEAT1.

Conclusion: Overexpression of GAS8-AS1 inhibits GBM cell proliferation and invasion by downregulating NEAT1.
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http://dx.doi.org/10.1002/brb3.2128DOI Listing
May 2021

Microscale mechanism of microstructure, micromorphology and Janus wettability of the banana leaf surface.

Micron 2021 Apr 22;146:103073. Epub 2021 Apr 22.

College of Engineering, South China Agricultural University, Guangzhou, 510642, China; Guangdong Provincial Key Laboratory of Conservation and Precision Utilization of Characteristic Agricultural Resources in Mountainous Areas, Jiaying University, Meizhou, 514015, China. Electronic address:

As a result of natural selection, the adaxial and abaxial sides of banana leaves show different wetting states and anisotropy. Janus wettability between the adaxial and abaxial sides of the banana leaf surface is revealed for the first time in this work. This has relevance for the preparation of bionic materials and an important role in the efficient and high-quality production management of pesticide spraying in banana orchards. The main purpose of this research is to analyze and study the microscale mechanism and coupling relationship between the Janus wettability of banana leaf surface and the microstructure and micromorphology. We adopt advanced modern instrument analysis technology, such as contact angle (CA) measurements, field emission scanning electron microscopy (FESEM), X-ray spectrometric analysis (EDS), and Fourier transform infrared spectroscopy (FTIR), and performed tests on the adaxial and abaxial sides of banana leaves to investigate the cause of Janus wettability. The results show that banana leaves exhibit different degrees of anisotropy, mainly due to the surface micromorphology. Banana leaves exhibit a hydrophilic Wenzel state on the adaxial side and a weakly hydrophobic Cassie-Baxter state on the abaxial side. We focused on studying the coupling effect and found that the main coupling element impacting the Janus wettability of the banana leaf surface is the nanopillars microstructure, and the secondary coupling element is the content of hydrophilic functional groups on the surface. This work may lead to the design and fabrication of Janus wetting surfaces by mimicking the nanopillar structure on banana leaf surfaces and help explore the potential application of efficient and high-quality pesticide spraying in banana orchards.
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http://dx.doi.org/10.1016/j.micron.2021.103073DOI Listing
April 2021

Sustainability efficiency assessment of listed companies in China: a super-efficiency SBM-DEA model considering undesirable output.

Environ Sci Pollut Res Int 2021 Apr 24. Epub 2021 Apr 24.

School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China.

Chinese listed companies play a vital role in national economy, which has contributed more than half of the gross domestic product. But the environmental pollution existed in the production and operation of companies has been ignored, so an objective and comprehensive efficiency assessment of Chinese listed companies were urgent. This paper constructed an index system based on green perspective, employed a super-efficiency slacks-based measure DEA (SBM-DEA) model considering undesirable outputs to assess the sustainability efficiency of Chinese listed companies. The results indicated that (1) the green sustainability efficiency of listed companies needed to be greatly improved. The average efficiency score of samples was 0.461 over 3 years, so the improvement potential was about 53.9%, only 23 companies were relatively effective. (2) The sustainability efficiency of companies has shown a slow upward trend in volatility since 2017, the non-daily consumer goods sector was the most efficient, while utilities were relatively inefficient. (3) When efficient and inefficient companies were compared, the latter were found to have significant input surplus, especially in water consumption. (4) The analysis of sensitivity on inputs and outputs showed that attention should be paid to water consumption and greenhouse gas emissions. (5) Spearman non-parametric test verified that company size and debt-paying ability were the implicit factors affecting company sustainability efficiency. The results of performance evaluation can not only provide a potential reference for the operation and management of listed companies in China, but also have guiding significance for local governments to strengthen the supervision of companies.
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http://dx.doi.org/10.1007/s11356-021-13997-1DOI Listing
April 2021

The first 90: Progress in HIV detection in Zhejiang Province, 2008-2018.

PLoS One 2021 8;16(4):e0249517. Epub 2021 Apr 8.

Department of AIDS/STD Control and Prevention, Zhejiang Provincial Center for Disease Control, Hangzhou, Zhejiang, People's Republic of China.

To analyze the results of HIV screening and the HIV-positive rate based on different HIV detection strategies in Zhejiang Province, China. Data were downloaded from the AIDS Prevention and Control Information System on May 1, 2019. HIV screening, prevalence, and incidence data were analyzed from 2008 to 2018. The incidence of HIV was calculated from the results of BED testing. SPSS software (ver. 19.0) was used for the analysis. The number of people screened for HIV increased by 229.7% from 2008 to 2018, while the incidence of HIV increased from 1.14‱ (2010) to 1.67‱ (2018), peak by 2015 (2.28‱). The proportion of people screened for HIV in medical institutions increased from 62.0% in 2008 to 67.1% in 2018, while of all positive tests, 47.9% were conducted at medical institutions in 2008, which increased to 63.2% in 2018. VCT and STD clinic attendees, who had only 4.5% of all those undergoing HIV tests, accounted for 23.7% of all HIV positive in 2018. The rate of HIV-positive people and incidence of HIV both increased in Zhejiang Province between 2008 and 2015. The most effective strategy for detecting HIV new cases is screening visitors to VCT and STD clinics.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0249517PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8031385PMC
April 2021

Phosphoproteome Profiling Revealed the Importance of mTOR Inhibition on CDK1 Activation to Further Regulate Cell Cycle Progression.

J Proteome Res 2021 Apr 2. Epub 2021 Apr 2.

Department of Cell Biology, Zhejiang University School of Medicine, Hangzhou 310058, Zhejiang, China.

The mammalian target of rapamycin (mTOR) functions as a critical regulator of cell cycle progression. However, the underlying mechanism by which mTOR regulates cell cycle progression remains elusive. In this study, we used stable isotope labeling of amino acids in cell culture with a two-step strategy for phosphopeptide enrichment and high-throughput quantitative mass spectrometry to perform a global phosphoproteome analysis of mTOR inhibition by rapamycin. By monitoring the phosphoproteome alterations upon rapamycin treatment, downregulation of mTOR signaling pathway was detected and enriched. Further functional analysis of phosphoproteome revealed the involvement of cell cycle events. Specifically, the elevated profile of cell cycle-related substrates was observed, and the activation of CDK1, MAPK1, and MAPK3 kinases was determined. Second, pathway interrogation using kinase inhibitor treatment confirmed that CDK1 activation operated downstream from mTOR inhibition to further regulate cell cycle progression. Third, we found that the activation of CDK1 following 4-12 h of mTOR inhibition was accompanied by the activation of the Greatwall-endosulfine complex. In conclusion, we presented a high-confidence phosphoproteome map inside the cells upon mTOR inhibition by rapamycin. Our data implied that mTOR inhibition could contribute to CDK1 activation for further regulating cell cycle progression, which was mediated by the Greatwall-endosulfine complex.
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http://dx.doi.org/10.1021/acs.jproteome.0c00848DOI Listing
April 2021

Targeting Lysosomal Degradation Pathways: New Strategies and Techniques for Drug Discovery.

J Med Chem 2021 Apr 25;64(7):3493-3507. Epub 2021 Mar 25.

State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, Innovation Center of Nursing Research, Nursing Key Laboratory of Sichuan Province, West China Hospital, and Collaborative Innovation Center of Biotherapy, Sichuan University, Chengdu 610041, China.

A series of tools for targeted protein degradation are inspiring scientists to develop new drugs with advantages over traditional small-molecule drugs. Among these tools, proteolysis-targeting chimeras (PROTACs) are most representative of the technology based on proteasomes. However, the proteasome has little degradation effect on certain macromolecular proteins or aggregates, extracellular proteins, and organelles, which limits the application of PROTACs. Additionally, lysosomes play an important role in protein degradation. Therefore, lysosome-induced protein degradation drugs can directly regulate protein levels , achieve the goal of treating diseases, and provide new strategies for drug discovery. Lysosome-based degradation technology has the potential for clinical translation. In this review, strategies targeting lysosomal pathways and lysosome-based degradation techniques are summarized. In addition, lysosome-based degrading drugs are described, and the advantages and challenges are listed. Our efforts will certainly promote the design, discovery, and clinical application of drugs associated with this technology.
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http://dx.doi.org/10.1021/acs.jmedchem.0c01689DOI Listing
April 2021

Identification of a bridge-specific intramolecular exciton dissociation pathway in donor-π-acceptor alternating conjugated polymers.

Nanoscale Res Lett 2021 Mar 20;16(1):51. Epub 2021 Mar 20.

Henan Key Laboratory of Infrared Materials and Spectrum Measures and Applications, School of Physics, Henan Normal University, Xinxiang, 453007, People's Republic of China.

Intramolecular exciton dissociation is critical for high efficient mobile charge carrier generations in organic solar cells. Yet despite much attention, the effects of π bridges on exciton dissociation dynamics in donor-π-acceptor (D-π-A) alternating conjugated polymers remain still unclear. Here, using a combination of femtosecond time-resolved transient absorption (TA) spectroscopy and steady-state spectroscopy, we track ultrafast intramolecular exciton relaxation dynamics in three D-π-A alternating conjugated polymers which were synthesized by Qin's group and named HSD-A, HSD-B, HSD-C. It is found that the addition of thiophene unit as π bridges will lead to the red shift of steady-state absorption spectrum. Importantly, we reveal the existence of a new intramolecular exciton dissociation pathway mediated by a bridge-specific charge transfer (CT') state with the TA fingerprint peak at 1200 nm in π-bridged HSD-B and HSD-C. This CT' state results in higher electron capture rates for HSD-B and HSD-C as compared to HSD-A. Depending on the proportion of CT' state and nongeminate recombination are important step for the understanding of high power conversion efficiencies in HSD-B than in HSD-C. We propose that this bridge-specific exciton dissociation pathway plays an important role in ultrafast intramolecular exciton dissociation of organic photovoltaic material D-π-A alternating conjugated polymers.
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http://dx.doi.org/10.1186/s11671-021-03507-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7981365PMC
March 2021

Integrative identification of the pathogenic role of a novel G6PD missense mutation c.697G>C.

Ann Transl Med 2021 Feb;9(3):194

Center of Clinical Molecular Medicine & Newborn Screening Center, Ministry of Education Key Laboratory of Child Development and Disorders; National Clinical Research Center for Child Health and Disorders (Chongqing), China International Science and Technology Cooperation base of Child development and Critical Disorders, Children's Hospital of Chongqing Medical University, Chongqing, China.

Background: Glucose-6-phosphate dehydrogenase (G6PD) deficiency is a hereditary disease caused by pathogenic mutations of . While most of the pathogenic variants of have been annotated, hemolysis of unknown etiology but analogous to that in G6PD deficiency persists, implying the existence of undocumented pathogenic variants. In our previous study, we reported four novel variants in China, for which the pathogenicity remains to be verified.

Methods: The variants were verified by exogenous expression in HEK-293 cells, and their functions were predicted by PolyPhen-2 and SIFT. The CRISPR/Cas9 system was exploited to edit the c.697G>C variant in HEK-293 cells and K562 cells. The expression of was detected by quantitative PCR (qPCR) and western blotting. The cell growth capacity was detected by the CCK-8 assay and crystal violet staining. The G6PD enzyme activity was reflected by the G6P/6PG ratio test. The apoptosis of cells was detected by Annexin V-APC/7-AAD staining. The secondary and crystallographic structures were denoted according to the literature and PyMOL software. The G6PD protein was purified from lysis of transformed Escherichia coli () cell with Ni-charged Resin Column. The enzymatic activity was detected at different temperatures.

Results: The G6PD activity of exogenous c.697G>C in HEK-293 cells was significantly lower than that of wild type (WT) , a finding that was consistent with the observation in clinical samples. The functional predictions conducted by different algorithms indicated the damage role of the c.697G>C variant in its enzymatic activity. We recapitulated the c.697G>C variant both in HEK-293 cells and K562 cells by adapting the CRISPR/Cas9 strategy. Using distinct cell lines expressing the c.697G>C variant endogenously, we confirmed the deteriorative role of the c.697G>C variant in its enzymatic activity. Regarding the secondary and crystallographic structure, we found a mutated amino acid approaching the structural NADP binding site. Finally, we demonstrated the c.697G>C variant compromised the thermal stability of G6PD protein.

Conclusions: Our data delineated the pathogenic role of c.697G>C variant for G6PD deficiency, implying the wide usage of CRISPR/Cas9 for genetic disease research.
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http://dx.doi.org/10.21037/atm-20-3941DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7940930PMC
February 2021

The Roles of the LIM Domain Proteins in Cardiac and Hematopoietic Morphogenesis.

Front Cardiovasc Med 2021 11;8:616851. Epub 2021 Feb 11.

Department of Biochemistry and Molecular Biology, College of Hengyang Medical, University of South China, Hengyang, China.

has been used as a model organism for study on development and pathophysiology of the heart. LIM domain proteins act as adaptors or scaffolds to promote the assembly of multimeric protein complexes. We found a total of 75 proteins encoded by 36 genes have LIM domain in by the tools of SMART, FLY-FISH, and FlyExpress, and around 41.7% proteins with LIM domain locate in lymph glands, muscles system, and circulatory system. Furthermore, we summarized functions of different LIM domain proteins in the development and physiology of fly heart and hematopoietic systems. It would be attractive to determine whether it exists a probable "LIM code" for the cycle of different cell fates in cardiac and hematopoietic tissues. Next, we aspired to propose a new research direction that the LIM domain proteins may play an important role in fly cardiac and hematopoietic morphogenesis.
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http://dx.doi.org/10.3389/fcvm.2021.616851DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7928361PMC
February 2021

Comparative validation of multi-instance instrument segmentation in endoscopy: Results of the ROBUST-MIS 2019 challenge.

Med Image Anal 2021 May 28;70:101920. Epub 2020 Nov 28.

National Center for Tumor Diseases (NCT), Partner Site Dresden, Germany: German Cancer Research Center, Im Neuenheimer Feld 460, 69120 Heidelberg, Germany; Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, Germany; Helmholtz Association/Helmholtz-Zentrum Dresden - Rossendorf (HZDR), Bautzner Landstraße 400, 01328 Dresden, Germany.

Intraoperative tracking of laparoscopic instruments is often a prerequisite for computer and robotic-assisted interventions. While numerous methods for detecting, segmenting and tracking of medical instruments based on endoscopic video images have been proposed in the literature, key limitations remain to be addressed: Firstly, robustness, that is, the reliable performance of state-of-the-art methods when run on challenging images (e.g. in the presence of blood, smoke or motion artifacts). Secondly, generalization; algorithms trained for a specific intervention in a specific hospital should generalize to other interventions or institutions. In an effort to promote solutions for these limitations, we organized the Robust Medical Instrument Segmentation (ROBUST-MIS) challenge as an international benchmarking competition with a specific focus on the robustness and generalization capabilities of algorithms. For the first time in the field of endoscopic image processing, our challenge included a task on binary segmentation and also addressed multi-instance detection and segmentation. The challenge was based on a surgical data set comprising 10,040 annotated images acquired from a total of 30 surgical procedures from three different types of surgery. The validation of the competing methods for the three tasks (binary segmentation, multi-instance detection and multi-instance segmentation) was performed in three different stages with an increasing domain gap between the training and the test data. The results confirm the initial hypothesis, namely that algorithm performance degrades with an increasing domain gap. While the average detection and segmentation quality of the best-performing algorithms is high, future research should concentrate on detection and segmentation of small, crossing, moving and transparent instrument(s) (parts).
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http://dx.doi.org/10.1016/j.media.2020.101920DOI Listing
May 2021

Complete mitochondrial genome and phylogenetic analysis of Huangshan Black chicken ().

Mitochondrial DNA B Resour 2021 Jan 27;6(1):243-244. Epub 2021 Jan 27.

College of Animal Science and Technology, Anhui Agricultural University, Hefei, P.R. China.

In this study, we sequenced the complete mitochondrial genome (mitogenome) of the Huangshan Black chicken (HBC). Results showed that the complete HBC mitogenome was 16,785 bp in size, comprising 22 transfer RNA genes, 2 ribosomal RNA genes, 13 protein-coding genes, and 1 non-coding control region (D-loop). The overall nucleotide composition was 32.5% for C, 30.3% for A, 23.7% for T, and 13.5% for G. Phylogenetic analysis showed that the HBC mitogenome was clustered with Xianju chicken, which belonged to the haplogroup D2. Our results therefore demonstrate that the origin of HBC corresponds to haplogroup D2 distribution and might have at least one maternal lineage originated from Southeast Asia.
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http://dx.doi.org/10.1080/23802359.2020.1860694DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7872527PMC
January 2021

Dietary Adherence, Self-Regulatory Fatigue and Trait Self-Control Among Chinese Patients with Peritoneal Dialysis: A Cross-Sectional Study.

Patient Prefer Adherence 2021 25;15:443-451. Epub 2021 Feb 25.

Nursing and Health School of Zhengzhou University, Zhengzhou, Henan, People's Republic of China.

Purpose: Little is known about factors that predict dietary adherence among Chinese patients undergoing peritoneal dialysis. We investigated whether self-regulatory fatigue and trait self-control influence dietary adherence among Chinese patients with peritoneal dialysis.

Methods: A total of 192 Chinese patients undergoing peritoneal dialysis were recruited at two peritoneal dialysis centers. The dietary adherence, trait self-control and self-regulatory fatigue of these patients were assessed using self-administered questionnaires. Clinical data were extracted from the hospital medical records. The significance of several social demographic factors on dietary adherence was analyzed using One-way ANOVA was used to analyze, whereas the association between dietary adherence and self-regulatory fatigue as well as trait self-control were analyzed using Pearson correlation. The independence association between dietary adherence and other influencing factors was assessed using Multivariable linear regression analyses.

Results: We found that peritoneal dialysis patients only moderately adhere to dietary prescriptions. The top three least adherences were observed for salt intake (1.89±0.36), face of difficulty (2.86±0.26) and fluid restriction adherence intake (2.97±0.30). Multivariable linear regression analysis revealed that education level (β=0.339, P<0.001), residence (β=-0.151, P=0.015), self-regulatory fatigue (β= -0.648, P<0.001), and trait self-control (β=0.118, P=0.022) were independent predictors of dietary adherence.

Conclusion: Education level and residence strongly influence dietary adherence among Chinese peritoneal dialysis patients. On the other hand, Self-regulatory fatigue and trait self-control are independent predictors of dietary adherence among peritoneal dialysis patients. These findings can guide the enhancement of dietary adherence of peritoneal dialysis patients.
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http://dx.doi.org/10.2147/PPA.S298231DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7920602PMC
February 2021

Development of small-molecule tropomyosin receptor kinase (TRK) inhibitors for fusion cancers.

Acta Pharm Sin B 2021 Feb 23;11(2):355-372. Epub 2020 May 23.

State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, and Collaborative Innovation Center of Biotherapy, Sichuan University, Chengdu 610041, China.

Tropomyosin receptor kinase A, B and C (TRKA, TRKB and TRKC), which are well-known members of the cell surface receptor tyrosine kinase (RTK) family, are encoded by the neurotrophic receptor tyrosine kinase 1, 2 and 3 ( and ) genes, respectively. TRKs can regulate cell proliferation, differentiation and even apoptosis through the RAS/MAPKs, PI3K/AKT and PLC pathways. Gene fusions involving act as oncogenic drivers of a broad diversity of adult and pediatric tumors, and TRKs have become promising antitumor targets. Therefore, achieving a comprehensive understanding of TRKs and relevant TRK inhibitors should be urgently pursued for the further development of novel TRK inhibitors for potential clinical applications. This review focuses on summarizing the biological functions of TRKs and fusion proteins, the development of small-molecule TRK inhibitors with different chemotypes and their activity and selectivity, and the potential therapeutic applications of these inhibitors for future cancer drug discovery efforts.
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http://dx.doi.org/10.1016/j.apsb.2020.05.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7893124PMC
February 2021

Prognosis of Ocular Tuberculosis Following Long-Term Antitubercular Therapy.

J Ocul Pharmacol Ther 2021 Feb 1. Epub 2021 Feb 1.

Department of Ophthalmology, Eye Institute, Eye and ENT Hospital, Fudan University, Shanghai, China.

This study presents clinical features and prognosis after long-term (12-18 months) antitubercular therapy (ATT) in patients with ocular tuberculosis (OTB) in East China, an endemic area of tuberculosis. This retrospective study reviewed data from OTB patients treated at the Eye and ENT Hospital of Fudan University from 2008 to 2018. All the patients completed a minimum follow-up of 6 months after the cessation of ATT. Sixty-six patients with OTB were studied. The ocular manifestations included retinal vasculitis (51.6%), choroiditis (24.2%), panuveitis (23.2%), intermediate uveitis (7.4%), scleritis (5.3%), anterior uveitis (2.1%), and optic neuropathy (1%). Except for two patients (ATT for 6 months), all other patients (64/66, 96.97%) received ATT for at least 12 months (6 patients for 12 months, 30 patients for 15 months, and 28 patients for 18 months). Treatment in conjunction with oral corticosteroids was used in 48 patients (72.7%). The average initial best-corrected visual acuity (BCVA) was 0.8 ± 0.64 (LogMAR), which improved to 0.31 ± 0.35 (LogMAR) at the last follow-up ( < 0.05). The final BCVA was significantly associated with the initial BCVA and the duration of clinical symptoms. A complete remission of uveitis was achieved in 97% of the patients. This study observed a favorable prognosis with long-term ATT regimens. Patients with better baseline visual acuity and a shorter duration of clinical symptoms before diagnosis had a better prognosis.
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http://dx.doi.org/10.1089/jop.2020.0100DOI Listing
February 2021

The effects of message framing on online health headline selection of female users: A moderation of approach/avoidance motivation.

Int J Med Inform 2021 Jan 20;148:104397. Epub 2021 Jan 20.

School of Information Management, Wuhan University, Wuhan, Hubei, 430072, China. Electronic address:

Background: More and more females are diagnosed with and die of cancers. Acquiring cancer-related information and enriching one's knowledge of cancers are important to cancer prevention and treatment. Effective online health headlines are indispensable to encouraging the reading of the hyperlinked health articles, especially those on daunting topics such as cancers.

Objective: This study aims to reveal how message framing, i.e., gain- or loss-framing, influences female users' selection of cancer-related health headlines at two levels, i.e., attention and behavior.

Methods: An eye-tracking experiment was conducted to capture female participants' attention and clicking behavior in response to cancer-related headlines manipulated in terms of message framing. The Stimulus-Organism-Response (S- O-R) framework was introduced to develop the research model that also took approach/avoidance motivation into account as moderator.

Results: Compared with loss-framed headlines, gain-framed ones attracted more and longer fixations (β = .09, p < .01; β = .12, p < .01) as well as more clicks (exp(B) = 1.76, p < .001), and they additionally evoked a higher level of pleasure (β = .50, p = .00) yet a lower level of arousal (β=-.16, p = .00). Arousal partially mediated the relationship between message framing and headline selection (β = .16, p = .00; β = .16, p = .00; exp(B) = 1.8, p = .00). The participants high in approach motivation devoted more attention to gain-framed headlines than to loss-framed ones (F(1,1333) = 15.74, p < .001; F(1,1333) = 31.94, p < .001).

Conclusion: Gain-framing is a preferred technique over loss-framing for online health information providers to create effective headlines of cancer-related information. Using gain-framed headlines helps alleviate cancer information avoidance and enrich people's knowledge of fatal diseases.
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http://dx.doi.org/10.1016/j.ijmedinf.2021.104397DOI Listing
January 2021

Microbial diversity characteristics and the influence of environmental factors in a large drinking-water source.

Sci Total Environ 2021 May 19;769:144698. Epub 2021 Jan 19.

Department of Environmental Science & Engineering, Fudan University, 2205 Songhu Road, Shanghai 200438, PR China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, PR China. Electronic address:

Although the influence of environmental factors on the microbial community in water sources is crucial, it is seldom evaluated. The seasonal relationship between microbial diversity of bacteria and fungi and environmental factors was investigated in a large drinking-water reservoir using Illumina MiSeq sequencing. Forty-one bacterial phyla and nine fungal phyla were analyzed in the Qingcaosha Reservoir, Shanghai, China. The predominant bacterial phyla were Actinobacteria, Proteobacteria, Bacteroidetes, and Cyanobacteria, with the maximum relative abundance of 46%, 36.6%, 16.1%, and 14.9%, respectively. Actinobacteria were observed to be the predominant bacterial phylum during spring and summer. The maximum relative abundance of unclassified fungi appeared in summer (98.8%), which was higher than that of Ascomycota and Basidiomycota (11.7% and 8.2%, respectively). Principal coordinate analysis (PCoA) results showed that the structural similarity in the bacterial community was greater during summer and winter; however, the fungal community exhibited a greater similarity during spring and summer. 2-Methylisoborneol (2-MIB), an olfactory compound produced by microorganisms, was detected at a concentration of 8.97 ng/L during summer, which was slightly lower than the olfactory threshold (10 ng/L). The positive correlation between Actinobacteria and unclassified fungi and 2-MIB (p < 0.05) confirmed that Actinobacteria and unclassified fungi produced 2-MIB. The chemical oxygen demand (COD) was 1.48-1.94 mg/L, and the maximum concentrations of total nitrogen (TN) and total phosphorus (TP) were 2.1 mg/L and 0.5 mg/L, respectively. Chloroflexi were negatively correlated with COD (p < 0.05) but positively correlated with TP (p < 0.01). Nitrospirae were negatively correlated with COD (p < 0.05), but positively correlated with TN (p < 0.05). Among the classified fungi, Rozellomycota, Basidiomycota (p < 0.05), and Chytridiomycota (p < 0.01) were positively correlated with TP. Therefore, the relative abundance of predominant bacteria was affected by various environmental factors; however, fungi were mainly influenced by TP.
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http://dx.doi.org/10.1016/j.scitotenv.2020.144698DOI Listing
May 2021

Malignancy Risk Stratification Prediction of Amorphous Calcifications Based on Clinical and Mammographic Features.

Cancer Manag Res 2021 12;13:235-245. Epub 2021 Jan 12.

Department of Radiology, Fudan University Shanghai Cancer Center, Shanghai, People's Republic of China.

Purpose: To explore the potential factors influencing the malignancy risk of amorphous calcifications and establish a predictive nomogram for malignancy risk stratification.

Patients And Methods: Consecutive mammograms from January 2013 to December 2018 were retrospectively reviewed. Traditional clinical features were recorded, and mammographic features were estimated according to the 5th BI-RADS. Included calcifications were randomly divided into the training and validation cohorts. A nomogram was developed to graphically predict the risk of malignancy (risk) based on stepwise multivariate logistic regression analysis. The discrimination and calibration performance of the model were assessed in both the training and validation cohorts.

Results: Finally, 1018 amorphous calcifications with final pathological results in 907 women were identified with a malignancy rate of 28.4% (95% CI: 25.7%, 31.3%). The malignancy rates of subgroups divided by the distribution of calcifications, quantity of calcifications, age, menopausal status and family history of cancer were significantly different. There were 712 cases and 306 cases in the training and validation cohorts. The prediction nomogram was finally developed based on four risk factors, including age and distribution, maximum diameter and quantity of calcifications. The AUC of the nomogram was 0.799 (95% CI: 0.761, 0.836) in the training cohort and 0.795 (95% CI: 0.738, 0.852) in the validation cohort.

Conclusion: On mammography, the distribution, maximum diameter and quantity of calcifications are independent predictors of malignant amorphous calcifications and can be easily obtained in the clinic. The nomogram developed in this study for individualized malignancy risk stratification of amorphous calcifications shows good discrimination performance.
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http://dx.doi.org/10.2147/CMAR.S286269DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7811441PMC
January 2021

Ultrasensitive, rapid and selective sensing of hazardous fluoride ion in aqueous solution using a zirconium porphyrinic luminescent metal-organic framework.

Anal Chim Acta 2021 Feb 11;1145:95-102. Epub 2020 Nov 11.

State Key Laboratory of Pollution Control and Resource Reuse/School of the Environment, Nanjing University, Jiangsu, 210046, China.

The development of a rapid and sensitive method for the detection of fluoride ion (F) in aqueous systems is of great significance for human health and environmental monitoring. In this study, a zirconium porphyrinic luminescent metal-organic framework (LMOF), PCN-222, was employed as a novel fluorescent probe for the ultrasensitive, rapid and selective detection of F in water. The PCN-222 probe was prepared by a facile solvothermal method. It exhibited good fluorescence stability and was highly stable in water. The fluorescence emission of PCN-222 could be effectively and selectively quenched by F due to the strong coordination affinity of F to the zirconium clusters in PCN-222. The proposed fluorescence method for F detection based on PCN-222 probe afforded a linear response range of 1-20 μmol/L and a very low detection limit (0.048-0.065 μmol/L) in reference to many reported F fluorescent probes. Moreover, a rapid response time (<10 s) was obtained due to the open and uniform pore structure of PCN-222 that allowed the fast diffusion of F to interact with the zirconium recognition sites. Finally, the PCN-222 probe was successfully applied for the fluorescence detection of F in real water samples. These results highlight the great application potential of LMOF in the sensing fields.
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http://dx.doi.org/10.1016/j.aca.2020.11.007DOI Listing
February 2021

Oxygen availability affects the synthesis of quorum sensing signal in the facultative anaerobe Novosphingobium pentaromativorans US6-1.

Appl Microbiol Biotechnol 2021 Feb 13;105(3):1191-1201. Epub 2021 Jan 13.

Zhejiang Provincial Key Laboratory of Organic Pollution Process and Control, Department of Environmental Science, College of Environmental and Resource Sciences, Zhejiang University, 866 Yuhangtang Road, Bulding NongB, Hangzhou, 310058, China.

Bacterial populations rely on quorum sensing (QS) to coordinate their behaviors and are often challenged by the fluctuation in oxygen concentrations in their habitats. Oxygen is a crucial factor that affects bacterial metabolism in multiple ways. However, little is known about whether and how oxygen availability affects QS activities. To fill this gap, we used the facultative anaerobe Novosphingobium pentaromativorans US6-1 as a model system, and observed that the QS signal acyl homoserine-lactones (AHLs) were produced only in anoxic environments, such as biofilm, or liquid medium that initially contained less than 2 mg/L dissolved oxygen, but not in highly oxic environments. Comparative transcriptome analysis revealed that oxygen availability significantly affected the physiological activities in US6-1, including fatty acid metabolism, oxidative phosphorylation, citrate cycle, QS activities, and flagellar assembly. The absence of AHLs in the oxic culture was not due to degradation, but to the very low expression of the AHL synthase gene novI. High concentration of NADH during the middle log phase under static cultivation may be a trigger for AHL synthesis. This is the first report that production of AHLs is coupled with anoxic metabolism in a facultative anaerobe, which extends our knowledge on factors affecting bacterial QS occurrence. KEY POINTS: • AHL production is anoxic cultivation related. • Oxygen availability affects AHL synthesis by influencing novI expression. • Oxygen availability changes many metabolism activities including NADH production.
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http://dx.doi.org/10.1007/s00253-021-11089-1DOI Listing
February 2021

Development and validation of a photographic atlas of food portions for accurate quantification of dietary intakes in China.

J Hum Nutr Diet 2021 Jan 6. Epub 2021 Jan 6.

Department of Maternal, Child and Adolescent Health, School of Public Health, Nanjing Medical University, Nanjing, China.

Background: Accurate estimation of food portion sizes remains an important challenge in dietary data collection. The present study aimed to develop a food atlas with adequate visual reference to improve the accuracy of dietary surveys in China.

Methods: A food atlas for dietary surveys in China was developed using three visual reference systems, namely, regularly placed food portions, the two-dimensional background coordinates and common objects known in daily life. The atlas was validated by estimating a meal before and after using the food atlas, and differences in weight estimation were compared using a paired t-test. In total, 50 college students participated in the study.

Results: After determination of food varieties; design of the food display; purchase, processing, cooking and weighing of food; photographing food; post-image processing and data processing, a total of 799 pictures of 303 types of food and two types of tableware were produced. The mean value of food weight estimated with the atlas was closer to the actual weight, and the variation range of these values was smaller and more stable than that estimated without the atlas. The differences estimated before and after using the atlas for all foods were significant (P < 0.05). Comparing the differences in weight before using the atlas, the error ranges of food samples were reduced.

Conclusions: A food atlas has been developed for a retrospective dietary survey in China, which can be used to enable a better understanding of nutritional adequacy in the Chinese population.
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http://dx.doi.org/10.1111/jhn.12844DOI Listing
January 2021

Multiple Single-Nucleotide Polymorphism Detection for Antimalarial Pyrimethamine Resistance via Allele-Specific PCR Coupled with Gold Nanoparticle-Based Lateral Flow Biosensor.

Antimicrob Agents Chemother 2021 02 17;65(3). Epub 2021 Feb 17.

Department of Human Parasitology, School of Basic Medical Sciences, Hubei University of Medicine, Shiyan, China

Molecular genotyping holds tremendous potential to detect antimalarial drug resistance (ADR) related to single nucleotide polymorphisms (SNPs). However, it relies on the use of complicated procedures and expensive instruments. Thus, rapid point-of-care testing (POCT) molecular tools are urgently needed for field survey and clinical use. Herein, a POCT platform consisting of multiple-allele-specific PCR (AS-PCR) and a gold nanoparticle (AuNP)-based lateral flow biosensor was designed and developed for SNP detection of the dihydrofolate reductase () gene related to pyrimethamine resistance. The multiple-AS-PCR utilized 3' terminal artificial antepenultimate mismatch and double phosphorothioate-modified allele-specific primers. The duplex PCR amplicons with 5' terminal labeled with biotin and digoxin are recognized by streptavidin (SA)-AuNPs on the conjugate pad and then captured by anti-digoxin antibody through immunoreactions on the test line to produce a golden red line for detection. The system was applied to analyze SNPs in Pfdhfr N51I, C59R, and S108N of 98 clinical isolates from uncomplicated malaria patients. Compared with the results from nested PCR followed by Sanger DNA sequencing, the sensitivity was 97.96% (96/98) for N51I, C59R, and S108N. For specificity, the values were 100% (98/98), 95.92% (94/98), and 100% (98/98) for N51I, C59R, and S108N, respectively. The limit of detection is approximately 200 fg/μl for plasmid DNA as the template and 100 parasites/μl for blood filter paper. The established platform not only offers a powerful tool for molecular surveillance of ADR but also is easily extended to interrelated SNP profiles for infectious diseases and genetic diseases.
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http://dx.doi.org/10.1128/AAC.01063-20DOI Listing
February 2021

Targeting de novo purine synthesis pathway via ADSL depletion impairs liver cancer growth by perturbing mitochondrial function.

Hepatology 2020 Dec 17. Epub 2020 Dec 17.

RNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA, 01605, USA.

Hepatocellular carcinoma (HCC) is among the most common cancer types worldwide; yet, patients with HCC have limited treatment options. There is an urgent need to identify new drug targets that specifically inhibit the growth of HCC cells. Here, we used a newly-engineered CRISPR library targeting ~2,000 druggable genes to perform a high throughput screen, and identified adenylosuccinate lyase (ADSL) - a key enzyme involved in the de novo purine synthesis pathway - as a potential drug target for HCC. ADSL has been implicated as a potential oncogenic driver in some cancers, but its role in liver cancer progression remains unknown. CRISPR-mediated knockout of ADSL impaired colony formation of liver cancer cells by affecting adenosine monophosphate (AMP) production. In the absence of ADSL, the growth of liver tumors is retarded in vivo. Mechanistically, we found that ADSL knockout caused S-phase cell cycle arrest, not by inducing DNA damage, but by impairing mitochondrial function. Using HCC patient data, we also revealed that high ADSL expression occurs during tumorigenesis and is linked to poor survival rate. In conclusion, our findings uncover the role of ADSL-mediated de novo purine synthesis in fueling mitochondrial ATP production to promote liver cancer cell growth. Targeting ADSL may be a therapeutic approach for HCC patients.
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http://dx.doi.org/10.1002/hep.31685DOI Listing
December 2020

The N2N3 domains of ClfA, FnbpA, and FnbpB in Staphylococcus aureus bind to human complement factor H, and their antibodies enhance the bactericidal capability of human blood.

J Biochem 2020 Dec 16. Epub 2020 Dec 16.

Host Defense Protein Laboratory, College of Pharmacy, Pusan National University, Busan, Republic of Korea.

In the complement system, the opsonin C3b binds to the bacterial cell surface and mediates the opsonophagocytosis. However, the cell wall protein SdrE of Staphylococcus aureus inhibits the C3b activity by recruiting the complement regulatory protein factor H (fH). SdrE binds to fH via its N-terminal N2N3 domain, which are also found in six other staphylococcal cell wall proteins. In this study, we report that not only the N2N3 domain of SdrE but also those of ClfA, FnbpA, and FnbpB can bind to fH. When immobilized on a microplate, the N2N3 domains recruited fH and enhanced the factor I (fI)-mediated cleavage of C3b. When mixed with fH and S. aureus cells, the N2N3 domains inhibited the fH binding to S. aureus cells and reduced the fI-mediated C3b cleavage on the bacterial cell surface. The F(ab)'2 fragments of the rabbit N2N3 antibodies also inhibited the fH-binding to the S. aureus cell surface. When added to human blood, the N2N3 antibodies or the N2N3 domain proteins significantly increased the bactericidal activity. Based on these results, we conclude that, in S. aureus, not only SdrE but also ClfA, FnbpA, and FnbpB can contribute to the inhibition of C3b-mediated opsonophagocytosis.
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http://dx.doi.org/10.1093/jb/mvaa142DOI Listing
December 2020

Molecular surveillance of anti-malarial resistance Pfdhfr and Pfdhps polymorphisms in African and Southeast Asia Plasmodium falciparum imported parasites to Wuhan, China.

Malar J 2020 Nov 25;19(1):434. Epub 2020 Nov 25.

Department of Human Parasitology, School of Basic Medical Sciences, Hubei University of Medicine, Shiyan, 442000, China.

Background: Anti-malarial drug resistance is a severe challenge for eventual control and global elimination of malaria. Resistance to sulfadoxine-pyrimethamine (SP) increases as mutations accumulate in the Pfdhfr and Pfdhps genes. This study aimed to assess the polymorphisms and prevalence of mutation in these genes in the Plasmodium falciparum infecting migrant workers returning to Wuhan, China.

Methods: Blood samples were collected for 9 years (2011-2019). Parasite genomic DNA was extracted from blood spots on filter paper. The mutations were evaluated by nested PCR and sequencing. The single-nucleotide polymorphisms (SNPs) and haplotypes of the Pfdhfr and Pfdhps genes were analysed.

Results: Pfdhfr codon 108 showed a 94.7% mutation rate, while for Pfdhps, the rate for codon 437 was 79.0%. In total, five unique haplotypes at the Pfdhfr locus and 11 haplotypes at the Pfdhps locus were found while the Pfdhfr-Pfdhps combined loci revealed 28 unique haplotypes. A triple mutant (IRNI) of Pfdhfr was the most prevalent haplotype (84.4%). For Pfdhps, a single mutant (SGKAA) and a double mutant (SGEAA) were detected at frequencies of 37.8 and 22.3%, respectively. Among the combined haplotypes, a quadruple mutant (IRNI-SGKAA) was the most common, with a 30.0% frequency, followed by a quintuplet mutant (IRNI-SGEAA) with a frequency of 20.4%.

Conclusion: The high prevalence and saturation of Pfdhfr haplotypes and the medium prevalence of Pfdhps haplotypes demonstrated in the present data will provide support for predicting the status and progression of antifolate resistance in malaria-endemic regions and imported malaria in nonendemic areas. Additional interventions to evaluate and prevent SP resistance should be continuously considered.
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http://dx.doi.org/10.1186/s12936-020-03509-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7691106PMC
November 2020

A Novel Approach to Quantitative Evaluation of Outer Retinal Lesions Via a New Parameter "Integral" in Spectral Domain Optical Coherence Tomography.

Transl Vis Sci Technol 2020 11 2;9(12). Epub 2020 Nov 2.

Department of Ophthalmology, Eye and ENT Hospital of Fudan University, Fudan University, Shanghai, China.

Purpose: The purpose of this study was to design a new parameter "integral" to quantitatively evaluate the spatial cumulative reflectivity of the outer retinal layers in optical coherence tomography (OCT), and to investigate its role in the detection of outer retinal diseases.

Methods: This was a cross-sectional study. Fovea-centered line OCT scans were performed on 60 eyes of 60 healthy volunteers and 44 eyes of 44 patients diagnosed with outer retinal diseases. The integrals of the ellipsoid zone (EZ) and interdigitation zone (IZ) were measured by respectively accumulating the grayscale values of all the pixels within the EZ and IZ at specified locations on the scanning lines, and were then adjusted by calculating their percentages on the outer retina. The integrals of the EZ and IZ were compared between the two groups.

Results: The integrals of the EZ and IZ were stably and normally distributed in the healthy eyes, and were significantly lower in eyes with outer retinal lesions than in healthy ones ( < 0.05). Moreover, the integrals of the EZ and IZ were correlated with best corrected visual acuity (BCVA; adjusted R = 0.620) and the presence of outer retinal lesions (Nagelkerke R = 0.767). The area under the receiver operating characteristic (ROC) curve was 0.954 (95% confidence interval [CI] = 0.918-0.990) when the integral was selected as a diagnostic variable.

Conclusions: Obtained from this novel quantification method, the new parameter integral was comparable between different individuals and had the potential to detect outer retinal abnormalities in reflectivity through OCT.

Translational Relevance: Our work verified the feasibility of the new image analysis technique in the detection of the diseases affecting the outer retina.
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http://dx.doi.org/10.1167/tvst.9.12.8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7645250PMC
November 2020

Inhibition of AURKB, regulated by pseudogene , confers synthetic lethality to PARP inhibition in skin cutaneous melanoma.

Am J Cancer Res 2020 1;10(10):3458-3474. Epub 2020 Oct 1.

Laboratory of Cancer Biology, Key Lab of Biotherapy in Zhejiang, Cancer Center of Zhejiang University, Sir Run Run Shaw Hospital, Medical School of Zhejiang University Hangzhou 310000, China.

Despite significant advances, skin cutaneous melanoma (SKCM) is a common life-threatening cancer worldwide. Recently, pseudogenes have been discovered to be functional in many physiological processes and the pathogenesis of various diseases, including cancer. However, their relevance to SKCM remains largely unknown. In this study, seven upregulated pseudogenes were identified based on TCGA data. Among them, was negatively correlated with the overall survival of SKCM patients. After constructing a pseudogene-miRNA-mRNA regulatory network, was found to regulate the expression of oncogene by serving as a ceRNA. Both genetic and chemical inhibition of AURKB reduced viability and induced apoptosis of melanoma cells. Interestingly, DNA repair pathway seems to be involved in the anti-tumor effect of AURKB inhibition. Indeed, a synergistic therapeutic effect of AURKB inhibition and PARP inhibitor was confirmed both in vitro and in vivo. In conclusion, AURKB plays an oncogenic role and is a novel therapeutic target in SKCM. The combination of AURKB inhibition and PARP inhibitor has a synergistic effect, representing a promising treatment for SKCM.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7642665PMC
October 2020

The pelvis urinary microbiome in patients with kidney stones and clinical associations.

BMC Microbiol 2020 11 5;20(1):336. Epub 2020 Nov 5.

Department of Urology, Affiliated Wuxi No.2 Hospital, Nanjing Medical University, Wuxi, 214002, China.

Background: The long-held notion that, without urinary tract or circulatory infection, bladder urine and blood are sterile biofluids has been disproven. There have been no previous reports on the kidney pelvis urinary microbiome after bladder disinfection in kidney stone patients. This study aimed to determine whether a kidney pelvis urinary microbiome is present after eliminating the influence of the bladder urinary microbiome, whether the microbiome composition is different in patients with stone kidney pelvis (SKP) and non-stone kidney pelvis (NSKP), and the correlation between SKP and patient clinical characteristics.

Results: Comparisons of bacterial diversity and community structure exhibited that urine in bladder was similar to SKP and NSKP. However, the comparisons showed that urine samples were different from blood. The most common operational taxonomic units were shared by all three types of urine samples. Corynebacterium was significantly higher in SKP compared to NSKP. Several bacteria were associated with patient characteristics, including Lactobacillus, which was positively correlated with fasting blood glucose, and Prevotella was negatively correlated with BMI. Lactobacillus was significantly higher in SKP compared to blood but not in NSKP compared to blood.

Conclusions: The composition of the kidney pelvis urinary microbiome after disinfection of the bladder and its similarity to the bladder microbiome indicate that bladder urine can be used to replace kidney pelvis urine in microbiome research. Additionally, the comparison of SKP and NSKP and clinical associations suggest that the occurrence of kidney stones is responsible for the SKP urinary microbiome.
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http://dx.doi.org/10.1186/s12866-020-01992-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7643416PMC
November 2020

Clearness of operating field: a surrogate for surgical skills on in vivo clinical data.

Int J Comput Assist Radiol Surg 2020 Nov 12;15(11):1817-1824. Epub 2020 Oct 12.

Peking University Cancer Hospital, Beijing, China.

Purpose: Automatic surgical skill assessment is an emerging field beneficial to both efficiency and quality of surgical education and practice. Prior works largely evaluate skills on elementary tasks performed in the simulation laboratory, which cannot fully reflect the variety of intraoperative circumstances in the real operating room. In this paper, we attempt to fill this gap by expanding surgical skill assessment onto a clinical dataset including fifty-seven in vivo surgeries.

Methods: To tackle the workflow and device constraints in the clinical setting, we propose a robust and non-interruptive surrogate for surgical skills, namely the clearness of operating field (COF), which shows strong correlation with overall skills and high inter-annotator consistency on our clinical data. Then, an automatic model based on neural networks is developed to regress surgical skills through the surrogate of COF using only video as input.

Results: The automatic model achieves 0.595 Spearman's correlation with the ground truth of overall technical skill, which even exceeds the human performance of junior surgeons. Moreover, an exploratory study is conducted to validate the skill predictions against the clinical outcomes of patients.

Conclusion: Our results demonstrate that the surrogate of COF is promising and the approach is potentially applicable to clinical practice.
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http://dx.doi.org/10.1007/s11548-020-02267-zDOI Listing
November 2020

The prevalence and correlates of oral sex among low-tier female sex workers in Zhejiang province, China.

PLoS One 2020 8;15(9):e0238822. Epub 2020 Sep 8.

Department of HIV/STD Control and Prevention, Center for Disease Control and Prevention of Zhejiang Province, Hangzhou, PR China.

Objectives: Oral sex and its associated factors among low-tier female sex workers (FSWs) have not been documented in the Chinese literature. Here, we report this perspective in this group.

Methods: The data were derived from a large cross-sectional study conducted among low-tier FSWs using a structured questionnaire in 21 counties in Zhejiang province, China. The prevalence of oral sex and its associated factors among 2645 low-tier FSWs were analyzed using bivariate and multivariate analysis.

Results: Of all participants, 579 (21.9%) had performed oral sex with clients over the previous month. Multivariate analysis revealed that oral sex is related to being unmarried, low income, early initiation of commercial sex, having conducted commercial sex in more counties, longer duration of commercial sex, larger number of clients, ever having engaged in anal sex, less use of condoms and oral contraceptives during the previous month, low rate of adoption for contraception at the present time, and STI-related symptoms during the previous half year.

Conclusion: Oral sex practitioners among low-tier FSWs in China are at a higher risk of STI, HIV, and unwanted pregnancy compared to those who did not engage in oral sex. Behavioral interventions carried out among low-tier FSWs should specifically target low-tier FSWs who practice oral sex, should carefully take into account the characteristics of these FSWs, provide risk awareness education and training for condom use negotiation, and promote the availability of condom and reproductive health care, timely diagnosis, and treatment of STIs.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0238822PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7478619PMC
November 2020