Publications by authors named "Tingting Dai"

47 Publications

The plant beneficial rhizobacterium FZB42 controls the soybean pathogen due to bacilysin production.

Appl Environ Microbiol 2021 Sep 22:AEM0160121. Epub 2021 Sep 22.

Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, 210037, Nanjing, China.

Soybean root rot caused by the oomycete is a serious soil-borne disease threatening soybean production in China. FZB42 is a model strain for Gram-positive plant growth-promoting rhizobacteria and is able to produce multiple antibiotics. In this study, we demonstrated that FZB42 can efficiently antagonize The underlying mechanism for the inhibition was then investigated. The FZB42 mutants deficient in the synthesis of lipopeptides (bacillomycin D and fengycin), known for antifungal activities, and polyketides (bacillaene, difficidin, and macrolactin), known for antibacterial activities, were not impaired in their antagonism toward ; in contrast, mutants deficient in bacilysin biosynthesis completely lost their antagonistic activities toward , indicating that bacilysin was responsible for the activity. Isolated pure bacilysin confirmed this inference. Bacilysin was previously shown to be antagonistic mainly toward prokaryotic bacteria rather than eukaryotes. Here, we found that bacilysin could severely damage the hyphal structures of and lead to the loss of their intracellular contents. A device was invented allowing interactions between and FZB42 on nutrient agar. In this manner, the effect of FZB42 on was studied by transcriptomics. FZB42 significantly inhibited the expression of genes related to growth, macromolecule biosynthesis, pathogenicity, and ribosomes. Among them, the genes for pectate lyase were the most significantly downregulated. Additionally, we showed that bacilysin effectively prevents soybean sprouts from being infected by and could antagonize diverse species, such as , , , , and, most importantly, . spp. are widespread eukaryotic phytopathogens and often extremely harmful. can infect many types of plants important to agriculture and forestry and thus cause large economic losses. Perhaps due to inappropriate recognition of as a common pathogen in history, research on the biological control of is limited. This study shows that FZB42 can antagonize various species and prevent the infection of soybean seedlings by . The antibiotic produced by FZB42, bacilysin, which was previously known to have antibacterial effectiveness, is responsible for the inhibitory action against . We further showed that some genes and pathways may be targeted in future biocontrol studies. Therefore, our data provide a basis for the development of new tools for the prevention and control of root and stem rot in soybean and other plant diseases caused by .
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http://dx.doi.org/10.1128/AEM.01601-21DOI Listing
September 2021

lncRNA FEZF1-AS1 regulates biological behaviors of cervical cancer by targeting miRNA-1254.

Food Sci Nutr 2021 Sep 14;9(9):4722-4737. Epub 2021 Jul 14.

Department of gynaecology and obstetrics Chongqing General Hospital University of Chinese Academy of Sciences Chongqing China.

Aim: The purpose of this research was to evaluate lncRNA FEZF1-AS1 in cervical cancer development and clinical significance.

Materials And Methods: Collecting cervical cancer tissues, measuring FEZF1-AS1 expression, and analysis correlation between FEZF1-AS1 and prognosis. In cell vitro study, using MTT assay to measure cell proliferation, evaluating cell apoptosis by flow cytometry, measuring cell invasion and migration by Transwell and wound healing assay; lncRNA FEZF1-AS1 and miR-1254 gene expressions were evaluated by RT-qPCR assay; relative protein (Smurf1, E-cadherin, Vimentin, N-cadherin, AKT, p-AKT, c-Myc, and ZEB1) expressions were measured by Western blot assay. The correlation among FEZF1-AS1, miR-1254, and Smurf1 were analysis by dual luciferase reporter gene assay.

Results: By clinical analysis, lncRNA FEZF1-AS1 was high expression in cervical cancer tissues and high expression was closely correlated with poor prognosis in cervical cancer patients. In vitro study, the SiHa and HeLa cell biologically including cell proliferation, migration, and invasion of si-FEZF1-AS1 group which knockdown lncRNA FEZF1-AS1 were significantly depressed ( < .001, respectively). However, with miR-1254 expression inhibiting, the cell biological activities were significantly increased in si-FEZF1-AS1+miRNA inhibitor groups ( < .001, respectively).

Conclusion: lncRNA FEZF1-AS1 might be an oncological role in cervical cancer; lncRNA FEZF1-AS1 knockdown had antitumor effects with miR-1254 activating in cervical cancer by in vitro study.
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http://dx.doi.org/10.1002/fsn3.2315DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8441442PMC
September 2021

An Improved Transformation System for Using Green Fluorescent Protein.

Front Microbiol 2021 5;12:682754. Epub 2021 Jul 5.

Department of Botany and Plant Pathology, Center for Genome Research and Biocomputing, Oregon State University, Corvallis, OR, United States.

is a destructive pathogen causing root rot and dieback diseases on hundreds of economically and ecologically important plant species. Effective transformation systems enable modifications of candidate genes to understand the pathogenesis of . A previous study reported a polyethylene glycol and calcium dichloride (PEG/CaCl)-mediated protoplast transformation method of . However, the virulence of the transformants was compromised. In this study, we selected ATCC 15400 as a suitable wild-type isolate for PEG/CaCl transformation using the green fluorescent protein after screening 11 isolates. Three transformants, namely, PcGFP-1, PcGFP-3, and PcGFP-5, consistently displayed a green fluorescence in their hyphae, chlamydospores, and sporangia. The randomly selected transformant PcGFP-1 was as virulent as the wild-type isolate in causing hypocotyl lesions on lupines. Fluorescent hyphae and haustoria were observed intracellularly and intercellularly in lupine tissues inoculated with PcGFP-1 zoospores. The potential application of this improved transformation system for functional genomics studies of is discussed.
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http://dx.doi.org/10.3389/fmicb.2021.682754DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8287854PMC
July 2021

The evaluation of the chemical quality and UV overall components dissolution consistency of preparation.

Anal Methods 2021 08;13(31):3479-3492

School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning 110032, China.

In recent years, new chemical technology and chemical analysis methods have been widely used for the quality control of medicine to provide security for human life. However, for the increasingly popular traditional Chinese medicine (TCM) preparations, the traditional quality control technology has been challenged due the presence of multi-components. To solve this problem, this study proposed a comprehensive evaluation strategy from two aspects: chemical quality and in vitro dissolution consistency. Zhenju antihypertensive tablet (FCIP), as the research object of this study, is a compound preparation of Flos Chrysanthemi Indici with moderate antihypertensive effect. For the chemical quality, the capillary electrophoresis fingerprint (CE-FP) was established based on the characteristic multi-wavelength averaging fusion profiling (CMW-AFP) strategy, which can fuse peaks in the CE chromatogram at several select characteristic wavelengths into one profile through an averaging algorithm. All samples were clarified into 5 quality grades using the systematic quantified fingerprint method, and showed significant difference among the four manufacturers. Combined with the accurate determination of the marker components, the CE-FP evaluation results can provide a guarantee for the chemical quality consistency. For the in vitro dissolution consistency, a UV overall components dissolution method was proposed. Meanwhile, in order to match the established UV overall dissolution system, two f2 factors (f2-R and f2-MI) were calculated to compare the dissolution profiles. By comparing the chemical and UV overall dissolution results plus the PCA analysis, the holistic quality of the FCIP samples of four manufacturers were obtained as MA > MC > MD > MB. The established evaluation system is also a suitable strategy for controlling the chemical quality and dissolution consistency of other TCM preparations.
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http://dx.doi.org/10.1039/d1ay00994jDOI Listing
August 2021

Eyelid Lymphatics: An Anatomical Study by Microdissection.

J Craniofac Surg 2021 Jun 30. Epub 2021 Jun 30.

Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine Shanghai Huamei Medical Cosmetology Hospital, Shanghai, China.

Objective: To obtain further understanding of the eyelid lymphatic anatomy.

Method: Thirty-two halves of eyelids from 16 fresh fetus cadavers were studied by microdissection using a mixture of 3% Prussian blue and chloroform to visualize the lymphatic vessels.

Results: Three layers of lymphatic plexuses were demonstrated in the eyelids: a superficial or preorbicularis muscle plexus; a pretarsal or postorbicular muscle plexus; and a deep or posttarsal plexus. Furthermore, communicating branches among these plexuses were also spotted.

Conclusions: The study results demonstrated the topographic distribution of the eyelid lymphatic vessels and confirmed the existence of communicating branches. This discovery will be conducive to understanding the route and mechanism by which inflammation of the eyelid spreads and cancer disseminates. It also provides anatomical insights to apply during eyelid surgery with regard to the prevention of possible eyelid lymphatic injury.
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http://dx.doi.org/10.1097/SCS.0000000000007759DOI Listing
June 2021

The Relationship Between the Gut Microbiome and Neurodegenerative Diseases.

Neurosci Bull 2021 Jul 3. Epub 2021 Jul 3.

State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, China.

Many recent studies have shown that the gut microbiome plays important roles in human physiology and pathology. Also, microbiome-based therapies have been used to improve health status and treat diseases. In addition, aging and neurodegenerative diseases, including Alzheimer's disease and Parkinson's disease, have become topics of intense interest in biomedical research. Several researchers have explored the links between these topics to study the potential pathogenic or therapeutic effects of intestinal microbiota in disease. But the exact relationship between neurodegenerative diseases and gut microbiota remains unclear. As technology advances, new techniques for studying the microbiome will be developed and refined, and the relationship between diseases and gut microbiota will be revealed. This article summarizes the known interactions between the gut microbiome and neurodegenerative diseases, highlighting assay techniques for the gut microbiome, and we also discuss the potential therapeutic role of microbiome-based therapies in diseases.
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http://dx.doi.org/10.1007/s12264-021-00730-8DOI Listing
July 2021

First Report of a Phytopythium litorale Species Causing Crown and Root Rot on Rhododendron pulchrum in China.

Plant Dis 2021 Jun 30. Epub 2021 Jun 30.

College of Forestry, Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China, NANJING, jiangsu, China;

During a survey of pathogenic oomycetes in Nanjing, China from June 2019 to October 2020, at least ten adjacent Rhododendron pulchrum plants at a Jiangjun Mountain scenic spot showed symptoms of blight, and crown and root discoloration . Symptomatic root tissues collected from three 6-year-old plants were rinsed with water, cut into 10-mm pieces, surface sterilized with 70% ethanol for 1 min, and plated onto 10% clarified V8 PARP agar (cV8A-PARP) containing pimaricin (20 mg/liter), ampicillin (125 mg/liter), rifampicin (10 mg/liter), and pentachloronitrobenzene (20 mg/liter). Four Pythium-like isolates were recovered after three days of incubation at 26°C, and purified using hyphal-tipping. Ten agar plugs (2×2 mm2) of each isolate were grown in 10 mL of 10% clarified V8 juice (cV8) in a 10 cm plate at 26°C for 3 days to produce mycelial mats, and then the cV8 was replaced with sterile water. To stimulate sporangial production, three to five drops of soil extract solution were added to each plate. Sporangia were terminal, ovoid to globose, and the size is 24 to 45.6 (mean 34.7) (n=10.8) in length x 23.6 to 36.0 (mean 29.8) (n=6.2) in width. Gametangia were not observed in cV8A or liquid media after 30 days. For colony morphology, the isolates were sub-cultured onto three solid microbial media (cV8A-PARP, potato dextrose agar, corn meal agar) . All isolates had identical morphological features in the three media. Complete ITS and partial LSU and cox2 gene regions were amplified using primer pairs ITS1/ITS4, NL1/NL4, and FM58/FM66 , respectively. The ITS, LSU, and cox2 sequences of isolate PC-dj1 (GenBank Acc. No. MW205746, MW208002, MW208003) were 100.00% (936/936 nt), 100.00% (772/772 nt), and 99.64% (554/556 nt) identical to those of JX985743, MT042003, and GU133521, respectively. We built a maximum-likelihood tree of Phytopythium species using the concatenated dataset (ITS, LSU, cox2) to observe interspecific differences. Based on the morphological characters and sequences, isolate PC-djl was identified as Phytopythium litorale . As the four isolates (PC-dj1, PC-dj2, PC-dj3 and PC-dj4) tested had identical morphological characters and molecular marker sequences, the pathogenicity of the representative isolate, PC-dj1, was tested using two inoculation methods on ten one-year-old R. pulchrum plants. For the first inoculation method, plants were removed from the pot, and their roots were rinsed with tap water to remove the soil. Each of these plants was placed in a glass flask containing 250 mL of sterile water and 10 blocks (10 x 10 mm2) of mycelial mats harvested from a three-day-old culture of P. litorale, while the other plant was placed in sterile water as a control, and incubated at 26°C. After three days, symptoms including crown rot, root rot and blight was observed on the inoculated plants whereas the control remained asymptomatic. For the second inoculation method, ten plants were dug up to expose the root ball. Ten three-day-old cV8A plugs (5×5 mm2) from a PC-dj1 culture or sterile cV8A plugs were evenly insert into the root ball of a plant before it was planted back into the original pots. Both plants were maintained in a growth chamber set at 26°C with a 12/12 h light/dark cycle and irrigated as needed. After 14 to 21 days, the inoculated plant had symptoms resembling those in the field , while the control plant remained asymptomatic. Each inoculation method was repeated at triplicate and the outcomes were identical. Phytopythium isolates with morphological features and sequences identical to those of PC-dj1 were recovered from rotted crown and root tissues of all inoculated plants. Previously, P. litorale was found causing diseases of apple and Platanus orientalis in Turkey, fruit rot and seedling damping-off of yellow squash in southern Georgia, USA. This is the first report of this species causing crown and root rot on R. pulchrum, an important ornamental plant species in China. Additional surveys are ongoing to determine the distribution of P. litorale in the city of Nanjing.
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http://dx.doi.org/10.1094/PDIS-04-21-0830-PDNDOI Listing
June 2021

Tricyclic or Pentacyclic D Units: Design of D-π-A-Type Copolymers for High Organic Photovoltaic Cells.

ACS Appl Mater Interfaces 2021 Jul 27;13(26):30756-30765. Epub 2021 Jun 27.

CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, China.

Although there are several electron-donating (D) units, only the classic benzo[1,2-b:4,5-b']dithiophenes (BDT) unit was utilized to develop D-π-A-type copolymers for high-voltage organic photovoltaic (OPV) cells. Hence, in this work, we chose two tricyclic D units, BDT and benzo[1,2-b:4,5-b']difurans (BDF), together with one pentacyclic ring, dithieno[2,3-d;2',3'-d']benzo[1,2-b;4,5-b']dithiophenes (DTBDT), to comprehensively study the effect of different D units on the optoelectronic properties and photovoltaic performance. By copolymerized with the benzo[1,2,3]triazole (BTA) electron-accepting unit, the final copolymers , , and were combined with a nonfullerene acceptor (NFA) according to the "Same-A-Strategy." As we preconceived, all the three single-junction OPV cells can obtain high open-circuit voltage () over 1.10 V. Although the tricyclic D unit of BDF exhibits a slightly lower of 1.12 V because of its mildly larger energy loss of 0.698 eV, its higher carrier mobilities and exciton dissociation efficiency strikingly boost the short-circuit current () and fill factor, which contribute to a comparable PCE of 10.04% with (10.10%). However, the DTBDT-based polymer shows the worst performance with a PCE of 6.78% and a of 1.14 V, owing to the higher bimolecular recombination and disordered molecular stacking. Our results indicate that tricyclic D units should be a better choice for constructing D-π-A-type polymers for high-voltage photovoltaic materials than the pentacyclic analogues.
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http://dx.doi.org/10.1021/acsami.1c08487DOI Listing
July 2021

Sphingomyelin synthase 2 but not sphingomyelin synthase 1 is upregulated in ovarian cancer and involved in migration, growth and survival via different mechanisms.

Am J Transl Res 2021 15;13(5):4412-4421. Epub 2021 May 15.

Department of Gynaecology, Renmin Hospital of Wuhan University Wuhan, China.

Sphingomyelin synthase 1 (SMS1) and 2 (SMS2) are two enzymes required for sphingomyelin synthesis, and their roles in tumor transformation and development have been recently recognized. In this work, we systematically evaluated the expression patterns of SMS1 and 2 in ovarian cancer patient samples and cell lines. Furthermore, we analyzed the functions of SMS2 and its underlying mechanisms. We observed a specific increase in SMS2 expression in ovarian cancer tissues compared to the adjacent normal ovary tissues in majority of patients' samples. This is regardless of their clinico-pathological characteristics. SMS1 expression was similar between ovarian cancer and its normal counterpart in 30 patients tested. The upregulation of SMS2 but not SMS1 was also reproducible in a panel of ovarian cancer cell lines. Functional analysis indicated that SMS2 plays a predominant role in promoting migration rather than proliferation in ovarian cancer. SMS2 depletion suppressed migration, growth and survival, and furthermore this was dependent on SMS2 baseline level in ovarian cancer cells. SMS2 inhibition significantly augmented cisplatin's efficacy. We further found that migration inhibition induced by SMS2 depletion was largely due to the suppression of RhoA/ROCK/LIMK/cofilin and RhoA/ROCK/FAK/paxillin pathways. In addition, lipid metabolism disruption, oxidative stress and damage, and impaired mitochondrial function contributed to the inhibitory effects of SMS2 depletion in ovarian cancer growth and survival. Our work demonstrates that SMS2 but not SMS1 is upregulated in ovarian cancer and involved in migration, growth and survival via different mechanisms. Our findings highlight the therapeutic value of SMS2 inhibition in the treatment of ovarian cancer.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8205710PMC
May 2021

Case Report: Infective Endocarditis Caused by in a Hemodialysis Patient.

Front Med (Lausanne) 2021 5;8:655640. Epub 2021 May 5.

Hospital Infection Control Center, Xiangya Hospital, Central South University, Changsha, China.

Fungal endocarditis (FE) is a rare but fatal disease. The incidence of infective endocarditis (IE) in hemodialysis patients with catheters is thought to be obviously higher than that in the general population. We reported a case of IE caused by () in a 36-year-old woman on hemodialysis. Because the blood cultures were persistently negative, so we used mNGS (Metagenomic next generation sequencing) for early clinical diagnosis. After treatment with voriconazole, the patient's condition improved rapidly. She continued oral voriconazole treatment 1 year after discharge and is in good condition. The diagnosis and treatment strategies of FE in hemodialysis patients were discussed.
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http://dx.doi.org/10.3389/fmed.2021.655640DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8131528PMC
May 2021

Nivolumab vs Pembrolizumab for Treatment of US Patients With Platinum-Refractory Recurrent or Metastatic Head and Neck Squamous Cell Carcinoma: A Network Meta-analysis and Cost-effectiveness Analysis.

JAMA Netw Open 2021 May 3;4(5):e218065. Epub 2021 May 3.

Medical Decision and Economic Group, Department of Pharmacy, South Campus, Ren Ji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.

Importance: Nivolumab and pembrolizumab are approved for treating platinum-refractory recurrent or metastatic head and neck squamous cell carcinoma (R/M HNSCC). Physicians and patients are uncertain which drug is preferable, rendering a cost-effectiveness comparison between them necessary.

Objective: To evaluate the cost-effectiveness of nivolumab vs pembrolizumab in treating platinum-refractory R/M HNSCC.

Design, Setting, And Participants: Both the network meta-analysis and cost-effectiveness analysis included patients from the CheckMate 141 and the KEYNOTE 040 phase 3 randomized clinical trials. The Checkmate 141 trial started on May 1, 2014, with the present analysis based on a September 2017 data cutoff. The KEYNOTE 040 trial started on November 17, 2014, with the present analysis based on a May 15, 2017, data cutoff. A bayesian network meta-analysis that included 856 patients was carried out, and a cost-effectiveness analysis that included 487 patients was conducted by developing a partitioned survival model, both between February and November 2020. The robustness of the model was assessed via 1-way, 2-way, and probabilistic sensitivity analyses; subgroup analyses were included; and scenario analyses were conducted to investigate the associations of dosage adjustment of nivolumab with cost-effectiveness.

Main Outcomes And Measures: Life-years, quality-adjusted life-years (QALYs), overall costs, and incremental cost-effectiveness ratios (ICERs) were measured.

Results: In the cost-effectiveness analysis that included 487 patients, for US health care payers, when nivolumab was administered based on patient weight (3 mg/kg biweekly), at a willingness-to-pay (WTP) threshold of $100 000 per QALY, the probability of nivolumab being cost-effective compared with pembrolizumab was 56%; at a WTP threshold of $150 000 per QALY, the probability was 62%. When nivolumab was administered at a fixed dose of 240 mg biweekly or 480 mg monthly, at a WTP threshold of $100 000 per QALY, the probability of nivolumab being cost-effective was 42% to 45%; at a WTP threshold of $150 000 per QALY, the probability was 52% to 55%.

Conclusions And Relevance: Findings from this network meta-analysis and cost-effectiveness analysis suggest considering both WTP threshold and patient body weight when choosing between nivolumab and pembrolizumab for the treatment of patients with platinum-refractory R/M HNSCC. When the WTP threshold was $100 000 per QALY, for patients weighing less than 72 kg, nivolumab (3 mg/kg, biweekly) was considered cost-effective; otherwise, pembrolizumab was preferable. When the WTP threshold was $150 000 per QALY, nivolumab (3 mg/kg biweekly) was considered cost-effective for patients weighing less than 75 kg; otherwise, fixed-dose nivolumab (240 mg biweekly or 480 mg monthly) provided more cost savings.
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http://dx.doi.org/10.1001/jamanetworkopen.2021.8065DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8103222PMC
May 2021

Fabrication of High Organic Solar Cells with a Non-Halogenated Solvent and the Effect of Substituted Groups for "Same-A-Strategy" Material Combinations.

ACS Appl Mater Interfaces 2021 May 28;13(18):21556-21564. Epub 2021 Apr 28.

CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, China.

We report a class of high-voltage organic solar cells (OSCs) processed by the environmentally friendly solvent tetrahydrofuran (THF), where four benzotriazole (BTA)-based p-type polymers (, , , and ) and a BTA-based small molecule are applied as p-type and n-type materials, respectively, according to "Same-A-Strategy" (SAS). The single-junction OSCs based on all four material blends exhibit a high open-circuit voltage () above 1.10 V. We systematically study the impact of the three different substituents (-OCH, -F, -Cl) on the BTA unit of the polymer donors. Interestingly, containing the unsubstituted BTA unit shows the efficient hole transfer and more balanced charge mobilities, thus leading to the highest power conversion efficiency (PCE) of 10.08% with a of 1.11 V and a of 13.68 mA cm. Due to the upshifted highest electron-occupied molecular orbital (HOMO) level and the weak crystallinity of the methoxy-substituted polymer , the resulting device shows the lowest PCE of 7.40% with a slightly decreased of 1.10 V. In addition, after the chlorination and fluorination, the HOMO levels of the donor materials and are gradually downshifted, contributing to increased values of 1.16 and 1.21 V, respectively. Our results prove that an unsubstituted p-type polymer can also afford high voltage and promising performance via non-halogenated solvent processing, which is of great significance for simplifying the synthesis steps and realizing the commercialization of OSCs.
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http://dx.doi.org/10.1021/acsami.1c03757DOI Listing
May 2021

Side chain engineering of copolymers based on benzotriazole (BTA) and dithieno[2,3-d;2',3'-d']benzo[1,2-b;4,5-b']dithiophenes (DTBDT) enables a high PCE of 14.6.

Nanotechnology 2021 Feb 22. Epub 2021 Feb 22.

National Center for Nanoscience and Technology, Haidian District, 100190, CHINA.

Dithieno[2,3-d;2',3'-d']benzo[1,2-b;4,5-b']dithiophenes (DTBDT) is a kind of prospective candidate for constructing donor-π-acceptor (D-π-A) copolymer donors applied in organic solar cells (OSCs), but restricted for its relatively poor photovoltaic performance compared with benzo[1,2-b;4,5-b']dithiophenes (BDT)-based analogue. Herein, three conjugated polymers (PE51, PE52 and PE53) based DTBDT and benzo[d][1,2,3]triazole (BTA) bearing different length of side alkyl chain unit were synthesized. The change of alkyl chain length can effectively affect energy level distribution, molecular stacking, miscibility and morphology with the non-fullerene acceptor of Y6, resulting in gradually increased open-circuit voltage (VOC) from 0.79 to 0.83 V, improved short-current density (JSC) and fill-factor (FF) reached up to 25.36 mA cm-2 and 71.94% in PE52-based device (with moderate alkyl chain). Impressively, compared with BDT-based analogue J52-Cl, the significantly enhanced crystallinity and intermolecular interaction of PE52 had effectively boosted the charge transport characteristic and optimized the surface morphology, thereby increasing the power conversion efficiency (PCE) from 12.3% to 14.6%, which is the highest PCE among DTBDT-based polymers and BTA-based polymers. Our results show that not only high efficiency could be achieved via using DTBDT as D unit, but also the length of the alkyl chain on BTA has a significant impact on photovoltaic performance for DTBDT-based polymers.
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http://dx.doi.org/10.1088/1361-6528/abe896DOI Listing
February 2021

Cationic covalent-organic framework for sulfur storage with high-performance in lithium-sulfur batteries.

J Colloid Interface Sci 2021 Jun 6;591:264-272. Epub 2021 Feb 6.

State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan Provincial Key Lab of Fine Chemistry, School of Chemical Engineering and Technology, Hainan University, Haikou 570228, China. Electronic address:

Covalent organic frameworks (COFs) with pre-designed structure and customized properties have been employed as sulfur storage materials for lithium-sulfur (Li-S) batteries. In this work, a cationic mesoporous COF (COF-NI) was synthesized by grafting a quaternary ammonium salt group onto the pore channel of COFs via a one-pot three components tandem reaction strategy. The post-functionalized COFs were utilized as the matrix framework to successfully construct the Li-S battery with high-speed capacity and long-term stability. The experimental results showed that, after loading active material sulfur, cationic COF-NI effectively suppressed the shuttle effect of the intermediate lithium polysulfide species in Li-S batteries, and exhibited better cycle stability than the as-obtained neutral COF (COF-Bu). For example, compared with COF-Bu based sulfur cathode (521 mA h g), the cationic COF-NI based sulfur cathode maintained a discharge capacity of 758 mA h g after 100 cycles. These results clearly showed that appropriate pore environment of COFs can be prepared by rational design, which can reduce the shuttle effect of lithium polysulfide species and improve the performance of Li-S battery.
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http://dx.doi.org/10.1016/j.jcis.2021.02.010DOI Listing
June 2021

The analysis of active compounds in by UHPLC Q exactive HF hybrid Quadrupole-Orbitrap MS and comprehensive quality assessment of its preparation.

Food Funct 2021 Mar;12(4):1769-1782

School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, China.

Flos Chrysanthemi Indici (FCI) is a common food and its preparation (FCIP) is generally made to realize the medicinal value of FCI in a more accurate and controllable way. In this paper, an efficient UHPLC Q Exactive HF Hybrid Quadrupole-Orbitrap MS method was exploited to elucidate the difference of chemical components between FCI and FCIP. Multi-fingerprints (HPLC-FP, UV-FP, and DSC-FP) were established with the evaluation results integrated by a weighted mean algorithm using variation coefficient (CVWM), which is more objective and reasonable. Through this method, the quality grades of 25 FCIP samples from four manufacturers were successfully discriminated. Meanwhile, the PLS model for the profile-efficacy relationship between the HPLC-FP and the antioxidant activities was established, as well as the correlation between the antioxidant ability (IC50) and the DSC curve (enthalpy values) were preliminary explored. In conclusion, this study provides a novel and holistic analytical strategy for food herbal remedies and its preparation.
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http://dx.doi.org/10.1039/d0fo03053hDOI Listing
March 2021

Balanophorin B inhibited glycolysis with the involvement of HIF-1α.

Life Sci 2021 Feb 24;267:118910. Epub 2020 Dec 24.

Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China; Nanjing Jinling Hospital, Nanjing, Jiangsu, China. Electronic address:

Aims: Cancer cells exhibit a metabolic change called aerobic glycolysis compared with normal cells. Balanophorin B is a terpenoid ingredient reported from the genus Balanophora. In this research, we studied the effect of balanophorin B on glycolysis of HepG2 cells and Huh-7 cells under hypoxia.

Main Methods: The Warburg effect was monitored by assessing glucose uptake, oxygen consumption rate (OCR) and extracellular acidification rate (ECAR). Key enzymes in the glycolytic pathway and HIF-1α protein expression and degradation were analyzed by real-time PCR and western blotting. The anti-cancer effect of balanophorin B in vivo was also investigated.

Key Findings: Balanophorin B inhibited the proliferation, glucose uptake, and ECAR in both HepG2 cells and Huh-7 cells. In addition, balanophorin B inhibited the protein level of HIF-1α and its downstream targets LDHA and HKII under hypoxia, whereas HIF-1α mRNA level did not change after balanophorin B treatment. The HIF-1α plasmid reversed the inhibition of balanophorin B on glycolysis, and the proteasome inhibitor MG132 attenuated the effect of balanophorin B on HIF-1α protein expression, suggesting that balanophorin B might post-transcriptionally affect HIF-1α. Moreover, balanophorin B increased the expression of VHL and PHD2. HIF-1α siRNA also greatly attenuated the inhibitory effect of balanophorin B on HepG2 cells glucose uptake. Balanophorin B significantly inhibited tumor growth in vivo, without causing obvious toxicity to mice.

Significance: These data suggest that balanophorin B inhibits glycolysis probably via an HIF-1α-dependent pathway, and the ubiquitin-proteasome pathway was greatly involved in the induction of balanophorin B on HIF-1α degradation.
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http://dx.doi.org/10.1016/j.lfs.2020.118910DOI Listing
February 2021

First Report of Phytophthora pini Causing Foliage Blight and Shoot Dieback of Rhododendron pulchrum in China.

Plant Dis 2020 Dec 2. Epub 2020 Dec 2.

College of Forestry, Nanjing Forestry University, Nanjing, JiangsuNANJING, jiangsu, China, 210037;

During a 2019-2020 survey for plant pathogenic oomycetes in Nanjing, China, severe foliage blight and dieback were observed on approximately 20 Rhododendron pulchrum plants at three public parks and gardens. Approximately 25% of leaves and shoots were affected. Symptoms included brown to black lesions on leaves and stems, dieback of shoot tips, and wilting. Diseased tissues were collected from a five-year-old shrub with typical disease symptoms at Xuanwuhu Park. They were cut into 10×10 mm2 squares, immersed in 70% ethanol for 30 sec, and placed onto fresh clarified V8 juice agar (cV8A) containing pimaricin, ampicillin, rifampicin, and pentachloronitrobenzene. Phytophthora-like hypae were transferred to new cV8A plates daily. A total of five isolates were obtained after five days of incubation at 25°C. After approximately 20 days, all isolates were identical in morphological traits including semi-papillate sporangia and gametangia (homothallic). Thirty sporangia of a representative isolate Ppi were randomly selected and examined. They were mostly ovoid and sometimes obpyriform, averaging 41.0 ± 3.9 × 24.8 ± 3.2 µm. Antheridia of 30 randomly selected gametangia were paragynous, averaging 16.7 ± 0.7 × 12.4 ± 1.5 µm. Average diameters of oogonia and plerotic oospores were 29.2 ± 0.3 µm and 26.4 ± 1.6 µm, respectively. Chlamydospores were not observed. The above morphological traits suggested the causal agent belonging to the "P. citricola-complex". Isolate Ppi was subjected to sequencing of the rDNA internal transcribed spacer (ITS) region and the ras-related GTP-binding protein 1 (Ypt1) gene. ITS sequence of Ppi (GenBank ACN. MT672594) has 100% identity to that of P. pini (MG865565). It has a 3-nt difference from the ITS sequences of P. acerina (MG518642) and P. citricola (MG865475) and a 4-nt difference from that of P. plurivora (FJ665225). Ypt1 sequence of Ppi (MT680000) has 100% identity to that of P. pini (MK058416). Pathogenicity of Ppi on R. pulchrum was tested using both detached-leaf and whole-plant assays. In the former assay, each of six asymptomatic leaves was symmetrically wounded at both sides using a sterile inoculation needle. A 5×5 mm2 Ppi-colonized cV8A plug was placed on each wound of five leaves. Sterile agar plugs were used for a control leaf. All six leaves were placed on a wet filter paper in a closed container at 25°C. This assay was repeated twice. On the fifth day, all inoculated leaves had necrotic tissues around the wounds, while the control leaves remained asymptomatic. In the whole-plant assay, 20-inch-tall plants were used. Five attached leaves and the twig base of each plant were wounded. A control plant was inoculated in the same manner above, while sterile agar plugs were used. Each plant was covered with a plastic bag and maintained at 25°C. Wet cotton balls were placed in the bags to maintain humidity. After two days, the bag containing cotton balls was removed. This assay was repeated three times. After two weeks, all three inoculated plants in the three replicated trials had severe foliage blight and dieback, whereas control plants remained healthy. Phytophthora isolates recovered from artificially inoculated tissues were identical to isolate Ppi in morphological characters. Rhododendron diseases caused by P. pini were reported in the USA and Finland . This is the first report of P. pini causing foliage blight and dieback on R. pulchrum, an important nursery and landscape plant in China. Additional surveys are ongoing to determine the distribution of this pathogen in Nanjing. Management programs are under development to contain the spread of P. pini and treat diseased plants.
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http://dx.doi.org/10.1094/PDIS-07-20-1422-PDNDOI Listing
December 2020

First Report of Phytopythium helicoides Causing Crown and Root Rot on Rhododendron pulchrum in China.

Plant Dis 2020 Oct 2. Epub 2020 Oct 2.

Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China, NANJING, jiangsu, China;

During a 2019-2020 survey of plant pathogenic oomycetes in Nanjing, China, a cluster of five adjacent Rhododendron pulchrum plants in Xuanwuhu Park exhibited symptoms including crown and root rot and wilting. foliage blight caused due to collar and had rotting crown and root tissues resultingrot foliage blight. Diseased roots were rinsed in water, cut into 10 mm pieces, immersed in 70% ethanol for 60 sec, and plated onto clarified V8 juice agar (cV8A) containingamended with pimaricin (20 mg/liter), ampicillin (125 mg/liter), rifampicin (10 mg/liter), and pentachloronitrobenzene (20 mg/liter). After three3 days of incubation at 26°C, Ffive Pythium-like isolatescoloniesisolates were obtained using hypalhyphal-tipping after 3 days of incubation at 25°C. Ten agar plugs (2×2 mm2) of each isolate were growntransferred into 10 mLl of 10% clarified V8 juice (cV8) in a 100 -mm plate at 26°C to produce mycelial mats. After 3three days, cV8 was replaced with sterile water. To stimulate sporangial production, 3-5 drops of soil extract solution were added to each plate. Five isolates had identical morphological features. Sporangia were terminal, ovoid to globose, andmeasuring 34.2 ± 6.2 µm (24.0-42.5 µm range) in length and 30.7 ± 6.6 µm (20.9-41.1 µm range) in width. Oogonia were not observed. The following primers were used to amplify the rDNA internal transcribed spacer (ITS) region and the mitochondrial cytochrome c oxidase subunit 1 (cox1COI) and 2 (cox2COII) genes  of from aA representative isolate, PH-C were amplified using the primer pairs ITS6 and ITS4 (Cooke et al. 2000), OomCoxI-Levup and OomCoxI-Levlo (Robideau et al. 2011) and Cox2-F and Cox2-RC4 (Hudspeth et al. 2000), respectivelyPhe-1. Isolate A xxx675 bp, xxx657 bp and 561xxx bp fragmentPH-C , respectively were amplified and had have identical sequences of the ITS (GenBank ACN. MT824568), and cox1 (MT834959), COI and cox2 COII genes the rDNA internal transcribed spacer (ITS) region and the mitochondrial cytochrome c oxidase subunit 1 and 2 genes (GenBank ACN. MT824568, MT834959, (MT834958, respectively) sequences identical to those of Phytopythium helicoides (MN541109, MK879709, KT595689, respectively). Based on the morphological and molecular characters, all five isolatesthe causal agent waswere identified the species represented by Phe-1 was identified as P. helicoides. One-year-old R. pulchrum plants (approx. 0.3 m in height) grown in 8×8 cm2 pots were used in to test the pathogenicity trials. Ten plants wasere carefully dug up to expose root ballsclusterballs. TenThree- days -old cultures of the isolate PH-Che-1 were used as the inoculum. Five The pplantss wereere inoculated by inserting 10 agar plugs into thee root ball of each plantcluster. For inoculatingfive control plants, sterile cV8A discsplugs were used. All inoculated plants were re-potted using original fresh potting mix and potsture .Ten 3-day-old cV8A cultural plugs (5×5 mm2) of Phe-1 were evenly insert into the root ball of each of five plants, while sterile cV8A plugs were used for five control plants. All were then planted into their original pots. Plants were maintained in a growth chamber set at 26°C with a 12/12 h light/dark cycle and irrigated as needed. After 21-25 days, the inoculated plants had symptoms identical to those in the field, while the controls remained asymptomatic. Identical outcomes were obtained from two repeated The pathogenicity trials. test was repeatedconducted twice . and the coutcome was identical. Phytopythium. helicoides (Phe-1) was reisolated from all symptomatic plants inemerging from the pathogenicity trials. Phytopythium helicoides was found causing diseases of Asian lotus (Yin et al. 2015), mandarin orange (Chen et al. 2016), and kiwifruit (Wang et al. 2015) plants in China. Phytopythium isolates with identical morphological features to those of Phe-1 were recovered from rotted crown and root tissues of all inoculated plants. In this note, P. helicoides causing crown and root rot on R. pulchrum is reported for the first time. Globally, this is the first report of P. helicoides causing crown blight and root rot of R. pulchrum. Additional surveys are being conducted forto mapping the distribution of P. helicoides in Nanjing, Province of China.
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http://dx.doi.org/10.1094/PDIS-08-20-1798-PDNDOI Listing
October 2020

A Rapid, Equipment-Free Method for Detecting in the Field Using a Lateral Flow Strip-Based Recombinase Polymerase Amplification Assay.

Plant Dis 2020 Nov 14;104(11):2774-2778. Epub 2020 Sep 14.

College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.

Late blight, caused by the oomycete , is a major constraint on the production of potatoes and tomatoes as well as a constant threat to global food security. An early diagnostic tool is important for the effective management of late blight in the field. Here, in combination with a simplified DNA extraction method, we developed a lateral flow strip-based recombinase polymerase amplification (LF-RPA) assay for the rapid, equipment-free detection of . . This assay targets the Ras-related protein () gene and can be performed over a wide range of temperatures (25 to 45°C). All 12 . isolates yielded positive detection results using the LF-RPA assay, and no cross-reaction occurred with related oomycetes or fungal species. With this assay, the detection limit was 500 fg of genomic DNA in optimized conditions. Furthermore, by combining a simplified polyethylene glycol-NaOH method for extracting DNA from plant samples, the entire LF-RPA assay enabled the detection of within 30 min with no specialized equipment. When applied to field samples, it successfully detected . in naturally diseased potato plants from eight different fields in China. Therefore, the LF-RPA assay is simple, rapid, and cost-effective and has potential for further development as a kit for diagnosing late blight in resource-limited settings or even on-site.
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http://dx.doi.org/10.1094/PDIS-01-20-0203-SCDOI Listing
November 2020

Xigua Video as a Source of Information on Breast Cancer: Content Analysis.

J Med Internet Res 2020 09 29;22(9):e19668. Epub 2020 Sep 29.

Department of Radiation Oncology, The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University, Jiangsu province, Huaian, China.

Background: Seeking health information on the internet is a popular trend. Xigua Video, a short video platform in China, ranks among the most accessed websites in the country and hosts an increasing number of videos with medical information. However, the nature of these videos is frequently unscientific, misleading, or even harmful.

Objective: Little is known about Xigua Video as a source of information on breast cancer. Thus, the study aimed to investigate the contents, quality, and reliability of breast cancer-related content on Xigua Video.

Methods: On February 4, 2020, a Xigua Video search was performed using the keyword "breast cancer." Videos were categorized by 2 doctors based on whether the video content provided useful or misleading information. Furthermore, the reliability and quality of the videos were assessed using the 5-point DISCERN tool and 5-point global quality score criteria.

Results: Out of the 170 videos selected for the study, 64 (37.6%) were classified as useful, whereas 106 (62.4%) provided misleading information. A total of 41.8% videos (71/170) were generated by individuals compared to 19.4% videos (33/170) contributed by health care professionals. The topics mainly covered etiology, anatomy, symptoms, preventions, treatments, and prognosis. The top topic was "treatments" (119/170, 70%). The reliability scores and global quality scores of the videos in the useful information group were high (P<.001). No differences were observed between the 2 groups in terms of video length, duration in months, and comments. The number of total views was higher for the misleading information group (819,478.5 vs 647,940) but did not reach a level of statistical significance (P=.112). The uploading sources of the videos were mainly health care professionals, health information websites, medical advertisements, and individuals. Statistical differences were found between the uploading source groups in terms of reliability scores and global quality scores (P<.001). In terms of total views, video length, duration, and comments, no statistical differences were indicated among the said groups. However, a statistical difference was noted between the useful and misleading information video groups with respect to the uploading sources (P<.001).

Conclusions: A large number of Xigua videos pertaining to breast cancer contain misleading information. There is a need for accurate health information to be provided on Xigua Video and other social media; health care professionals should address this challenge.
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http://dx.doi.org/10.2196/19668DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7574999PMC
September 2020

A Fluorogenic Trehalose Probe for Tracking Phagocytosed .

J Am Chem Soc 2020 09 27;142(36):15259-15264. Epub 2020 Aug 27.

Department of Chemistry, Stanford University, Stanford, California 94305, United States.

Tuberculosis (TB) disease is a global epidemic caused by the pathogenic (Mtb). Tools that can track the replication status of viable Mtb cells within macrophages are vital for the elucidation of host-pathogen interactions. Here, we present a cephalosphorinase-dependent green trehalose (CDG-Tre) fluorogenic probe that enables fluorescence labeling of single live Bacille Calmette-Guérin (BCG) cells within macrophages at concentrations as low as 2 μM. CDG-Tre fluoresces upon activation by BlaC, the β-lactamase uniquely expressed by Mtb, and the fluorescent product is subsequently incorporated within the bacterial cell wall via trehalose metabolic pathway. CDG-Tre showed high selectivity for mycobacteria over other clinically prevalent species in the suborder. The unique labeling strategy of BCG by CDG-Tre provides a versatile tool for tracking Mtb in both pre- and postphagocytosis and elucidating fundamental physiological and pathological processes related to the mycomembrane.
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http://dx.doi.org/10.1021/jacs.0c07700DOI Listing
September 2020

Identification of possible reductants in the aqueous leaf extract of mangrove plant Rhizophora apiculata for the fabrication and cytotoxicity of silver nanoparticles against human osteosarcoma MG-63 cells.

Mater Sci Eng C Mater Biol Appl 2020 Nov 3;116:111252. Epub 2020 Jul 3.

Jiangsu Province Hospital of Chinese Medicine, Nanjing 210000, PR China; Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210000, PR China. Electronic address:

Rhizophora apiculata is a less studied tannin-rich plant of the mangrove ecosystem with potent biomedical applications. Tannins have been known to reduce silver ions into silver nanoparticles which in particular are known to possess cytotoxic effects against a variety of cancer cells. The aqueous leaf extract was prepared and quantitatively analyzed for its phytochemical content. According to the quantitative phytochemical analysis, the extract was rich in tannins and other reducing sugars. The reducing sugar-rich extract was further used for the synthesis of silver nanoparticles. Taking these facts into consideration, in this study, an eco-friendly approach was followed to biosynthesize silver nanoparticles using a tannin-rich Rhizophora apiculata aqueous leaf extract. The synthesized nanoparticles were partially characterized by our previous reports. This report further characterizes the particles by determining its average size, polydispersity index and zeta potential using dynamic light scattering. After characterization, the nanoparticles were tested for cytotoxic effects against human osteosarcoma MG-63 cells. The effects were analyzed by microscopic observation and MTT assay. The results indicate that the tannin-rich extract reduced the precursor silver nitrate into silver nanoparticles of favorable size for tumor infiltration. The nanoparticles possessed significant cytotoxic effects against MG-63 cells which could be possibly attributed to the antioxidant activity of silver nanoparticles. Further studies at the molecular level can indicate its potential in nanomedicine for the treatment of bone cancer at the clinical level.
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http://dx.doi.org/10.1016/j.msec.2020.111252DOI Listing
November 2020

Integration of antimicrobial peptides and gold nanorods for bimodal antibacterial applications.

Biomater Sci 2020 Aug;8(16):4447-4457

Department of Infectious Disease, Shulan (Hangzhou) Hospital Affiliated to Zhejiang Shuren University, Shulan International Medical College, Hangzhou 310022, China and State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310022, China.

The misuse and abuse of antibiotics have given rise to a severe problem of the drug resistance of bacteria. Solving this problem has been a vitally important task in the modern medical arena. In this work, an antimicrobial peptide (AMP), BF2b, and gold nanorods (AuNRs) were used to develop a specific drug delivery system for killing methicillin-resistant Staphylococcus aureus (MRSA). On the one hand, BF2b has unique anti-bacterial performance and has a lower tendency than traditional antibiotics to engender the drug resistance of bacteria. On the other hand, AuNRs have diverse distinct properties, such as photo-thermal conversion, which can be employed for photo-thermal sterilization. We aimed to integrate the anti-bacterial activity of BF2b and the photo-thermal sterilization of AuNRs to kill drug-resistant bacteria. Fourier-transform infrared spectroscopy, microBCA and zeta potential measurements were utilized to characterize the product, [email protected]/BF2b. Transmittance electron microscopy, UV-vis spectroscopy and photothermal conversion measurement were conducted to verify the stability and photothermal conversion capacity of [email protected]/BF2b. Cell viability and hemolysis assay were carried out to test the biocompatibility of [email protected]/BF2b. Finally, the in vitro and in vivo experiments were performed to demonstrate the excellent bactericidal activity of [email protected]/BF2b.
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http://dx.doi.org/10.1039/d0bm00782jDOI Listing
August 2020

Near-Infrared Fluorescent and Magnetic Resonance Dual-Imaging Coacervate Nanoprobes for Trypsin Mapping and Targeted Payload Delivery of Malignant Tumors.

ACS Appl Mater Interfaces 2020 Apr 2;12(15):17302-17313. Epub 2020 Apr 2.

The State Key Laboratory of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, P. R. China.

Trypsin-responsive near-infrared fluorescent (NIRF) and magnetic resonance (MR) dual-imaging composite nanoparticle/polypeptide coacervate nanoprobes with tunable sizes, have been constructed herein via electrostatic interaction-induced self-assembly. Considering the requirements of in vivo metabolism on nanoparticle size, three coacervate nanoprobes with diameters of around 100, 200, and 300 nm were fabricated with a polydispersity of around 0.2. These coacervate nanoprobes consist of FeO magnetic nanoparticles surface-decorated with poly acrylic acid and Cy5.5-modified poly-l-lysine (PLL--Cy5.5) serving as MR imaging and trypsin-responsive substrate/NIRF agents, respectively. The notable fluorescence signal from PLL--Cy5.5 is self-quenched due to the short distances between the fluorescent Cy5.5 molecules after construction of the coacervate nanoprobes. Remarkably, coacervate nanoprobes with a diameter of around 100 nm are selectively disintegrated into fragmented segments upon the hydrolysis of PLL by trypsin, resulting in an 18-fold amplification of the NIRF intensity in comparison with the self-assembled coacervate nanoprobes in the quenched state. Moreover, the MR imaging enhancement is also related to the disintegration of the coacervate nanoprobes. Cellular experiments and in vivo studies demonstrate that the coacervate nanoprobes exhibit remarkable trypsin-sensitive NIRF and MR dual-imaging capabilities and thus have excellent potential to serve as dual-imaging nanoprobes for the efficient mapping of malignant tumors in which trypsin is often overexpressed. In consideration of their excellent capability to enrich charged molecules, the coacervate nanoprobes provide a conceptually novel and promising platform toward in vivo trypsin mapping and controlled delivery of targeted payloads.
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http://dx.doi.org/10.1021/acsami.0c03433DOI Listing
April 2020

The Roles of Podoplanin-Positive/Podoplanin-Negative Cells from Adipose-Derived Stem Cells in Lymphatic Regeneration.

Plast Reconstr Surg 2020 Feb;145(2):420-431

From the Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering; the Department of Surgery, Xiangya School of Medicine, Central South University; and the Division of Plastic Surgery, Lahey Hospital and Medical Center.

Background: Secondary lymphedema is a refractory disease, for which adipose-derived stem cells have shown some therapeutic potential. However, the mechanism of this action remains poorly understood.

Methods: The authors identified podoplanin-expressing adipose-derived stem cells, which allowed them to divide adipose-derived stem cells into podoplanin-positive and podoplanin-negative groups that they characterized in vitro. The authors then used a mouse hindlimb model for lymphedema to trace the fate of podoplanin-positive, podoplanin-negative, and unsorted adipose-derived stem cells in vivo.

Results: When induced in culture, podoplanin-positive cells were noted to up-regulate the expression of lymphatic endothelial cell markers, including LYVE-1, and assumed a cobblestone morphology. In addition, a substantial increase in lymphangiogenic cytokines was detected in the podoplanin-positive supernatant. The above findings were largely absent from the podoplanin-negative and unsorted groups. In the mouse model, the implanted cells relieved the limb lymphedema by promoting lymphangiogenesis, with the podoplanin-positive group showing the most significant effect. Immunocolocalization further revealed that the podoplanin-positive cells incorporated into lymphatic vessels were positive for LYVE-1.

Conclusions: These data demonstrated that actions by means of both paracrine and differentiation pathways were involved in the adipose-derived stem cell-mediated therapeutic effects. The podoplanin-positive cells possessed lymphatic paracrine and differentiation abilities and may represent lymphatic endothelial cell precursor cells. The podoplanin-negative cells, which constitute a considerable proportion of the adipose-derived stem cells, may play an important paracrine role by secreting mesenchymal stem cell-related factors.
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http://dx.doi.org/10.1097/PRS.0000000000006474DOI Listing
February 2020

Targeting MMP-14 for dual PET and fluorescence imaging of glioma in preclinical models.

Eur J Nucl Med Mol Imaging 2020 06 26;47(6):1412-1426. Epub 2019 Nov 26.

Department of Otolaryngology, University of Alabama at Birmingham, Birmingham, AL, 35294, USA.

Purpose: There is a clinical need for agents that target glioma cells for non-invasive and intraoperative imaging to guide therapeutic intervention and improve the prognosis of glioma. Matrix metalloproteinase (MMP)-14 is overexpressed in glioma with negligible expression in normal brain, presenting MMP-14 as an attractive biomarker for imaging glioma. In this study, we designed a peptide probe containing a near-infrared fluorescence (NIRF) dye/quencher pair, a positron emission tomography (PET) radionuclide, and a moiety with high affinity to MMP-14. This novel substrate-binding peptide allows dual modality imaging of glioma only after cleavage by MMP-14 to activate the quenched NIRF signal, enhancing probe specificity and imaging contrast.

Methods: MMP-14 expression and activity in human glioma tissues and cells were measured in vitro by immunofluorescence and gel zymography. Cleavage of the novel substrate and substrate-binding peptides by glioma cells in vitro and glioma xenograft tumors in vivo was determined by NIRF imaging. Biodistribution of the radiolabeled MMP-14-binding peptide or substrate-binding peptide was determined in mice bearing orthotopic patient-derived xenograft (PDX) glioma tumors by PET imaging.

Results: Glioma cells with MMP-14 activity showed activation and retention of NIRF signal from the cleaved peptides. Resected mouse brains with PDX glioma tumors showed tumor-to-background NIRF ratios of 7.6-11.1 at 4 h after i.v. injection of the peptides. PET/CT images showed localization of activity in orthotopic PDX tumors after i.v. injection of Ga-binding peptide or Cu-substrate-binding peptide; uptake of the radiolabeled peptides in tumors was significantly reduced (p < 0.05) by blocking with the non-labeled-binding peptide. PET and NIRF signals correlated linearly in the orthotopic PDX tumors. Immunohistochemistry showed co-localization of MMP-14 expression and NIRF signal in the resected tumors.

Conclusions: The novel MMP-14 substrate-binding peptide enabled PET/NIRF imaging of glioma models in mice, warranting future image-guided resection studies with the probe in preclinical glioma models.
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http://dx.doi.org/10.1007/s00259-019-04607-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7968515PMC
June 2020

A recombinase polymerase amplification-lateral flow dipstick assay for rapid detection of the quarantine citrus pathogen in China, .

PeerJ 2019 18;7:e8083. Epub 2019 Nov 18.

Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, Jiangsu, China.

, the causal agent of brown rot of citrus fruit, is an important worldwide pathogen and a quarantine pest in China. Current diagnosis of the disease relies on disease symptoms, pathogen isolation and identification by DNA sequencing. However, symptoms caused by can be confused with those by other and fungal species. Moreover, pathogen isolation, PCR amplification and sequencing are time-consuming. In this study, a rapid assay including 20-min recombinase polymerase amplification targeting the gene and 5-min visualization using lateral flow dipsticks was developed for detecting . This assay was able to detect 0.2 ng of genomic DNA in a 50-µL reaction system. It was specific to without detection of other tested species including , , and , four other important citrus pathogens. Using this assay, was also detected from artificially inoculated orange fruits. Results in this study indicated that this assay has the potential application to detect at diagnostic laboratories and plant quarantine departments of customs, especially under time- and resource-limited conditions.
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http://dx.doi.org/10.7717/peerj.8083DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6870529PMC
November 2019

A Novel LAMP Assay for the Detection of Utilizing a New Target Gene Identified From Genome Sequences.

Plant Dis 2019 Dec 15;103(12):3101-3107. Epub 2019 Oct 15.

Lianyungang Customs (formerly Lianyungang Entry-Exit Inspection and Quarantine Bureau), Lianyungang, China.

is an ecologically and agriculturally significant plant pathogen. Early and accurate detection of is paramount to disease prevention and management. In this study, a loop-mediated isothermal amplification (LAMP) assay utilizing a new target gene identified from genomic sequence data was developed and evaluated for the detection of . This LAMP assay was found highly specific to . All 10 tested isolates of yielded positive results, whereas 50 isolates belonging to 16 other species, , and 14 fungal species lacked detection. This assay was 10 times more sensitive (100 pg in a 25-µl reaction mixture) than a conventional PCR assay (2 ng in a 50-µl reaction mixture) for detecting the genomic DNA of . In addition, it detected from artificially inoculated leaves of . Moreover, detection rates of using environmental DNAs extracted from 13 naturally infested rhizosphere samples were 100% in the LAMP assay versus 46% in the conventional PCR assay. Considering its higher accuracy and shorter time span, this LAMP assay is a promising diagnostic tool to replace conventional PCR-based and culture-dependent assays for screening of in regions at risk of infection or contamination.
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http://dx.doi.org/10.1094/PDIS-04-19-0781-REDOI Listing
December 2019

Comparative Evaluation of a Novel Recombinase Polymerase Amplification-Lateral Flow Dipstick (RPA-LFD) Assay, LAMP, Conventional PCR, and Leaf-Disc Baiting Methods for Detection of .

Front Microbiol 2019 9;10:1884. Epub 2019 Aug 9.

Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.

Early and accurate detection of the causal pathogen is crucial for effective prevention and control of root and stem rot and seedling damping-off of soybean. In the present study, a novel isothermal amplification assay was developed for detecting . This 25 min assay included a two-step approach. First, a pair of novel primers, PSYPT-F and PSYPT-R were used to amplify a specific fragment of the gene of in a 20 min recombinase polymerase amplification (RPA) step. Second, lateral flow dipsticks (LFD) were used to detect and visualize RPA amplicons of within 5 min. This RPA-LFD assay was specific to . It yielded negative detection results against 24 other , one , and 14 fungal species. It was also found to be sensitive, detecting as low as 10 pg of genomic DNA in a 50-μL reaction. Furthermore, was detected from artificially inoculated hypocotyls of soybean seedlings using this novel assay. In a comparative evaluation using 130 soybean rhizosphere samples, this novel assay consistently detected in 55.4% of samples, higher than other three methods, including loop-mediated isothermal amplification (54.6%), conventional PCR (46.9%), and leaf-disc baiting (38.5-40.0%). Results in this study indicated that this rapid, specific, and sensitive RPA-LFD assay has potentially significant applications to diagnosing Phytophthora root and stem rot and damp-off of soybean, especially under time- and resource-limited conditions.
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http://dx.doi.org/10.3389/fmicb.2019.01884DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6696978PMC
August 2019

Novel Hybrid Dextran-Gadolinium Nanoparticles as High-relaxivity T1 Magnetic Resonance Imaging Contrast Agent for Mapping the Sentinel Lymph Node.

J Comput Assist Tomogr 2019 Mar/Apr;43(2):350-357

Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering

Objectives: To assess the applicability of a novel hybrid dextran-gadolinium nanoparticles (NPs) as high-relaxivity T1 magnetic resonance imaging (MRI) contrast agent for mapping the sentinel lymph node (SLN).

Methods: Dextran-bis-acrylamide-polyacrylic acid (Dex-MBA-PAA) NPs were synthesized through a self-assembly assisted approach and complexed with multiple chelated gadolinium (Gd) (III) ions. After their characterization was validated, they were used to mapping SLNs by MRI in Wistar rats, and their biosafety was evaluated.

Results: Dextran-MBA-polyacrylic acid-Gd NPs have suitable particle size and much higher longitudinal relaxivity (r1) than that of commonly used clinical MRI contrast agents (eg, gadopentetic acid dimeglumine salt injection). The in vivo T1-weighted MRI results revealed their effectiveness at mapping SLNs. And their biological safety was also verified.

Conclusions: Dextran-MBA-polyacrylic acid-Gd NPs were synthesized and validated by in vitro and in vivo experiments for their ability to visualize SLNs by MRI with accurate positioning and excellent biosafety, and they have great potential for clinical SLN mapping.
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http://dx.doi.org/10.1097/RCT.0000000000000842DOI Listing
March 2019
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