Publications by authors named "Tingchun Li"

4 Publications

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Systematic Analysis of MYB Genes Family in Acer rubrum and Functional Characterization of ArMYB89 Gene.

J Exp Bot 2021 May 16. Epub 2021 May 16.

Institute of Agricultural Engineering, Anhui Academy of Agricultural Sciences, Hefei, China.

The MYB transcription factor (TF) family is extensively distributed across the plant kingdom. However, the functional significance of red maple MYB TFs remains unclear. Our research identified 393 MYB transcription factors in the Acer rubrum genome, and these ArMYB members were unevenly distributed across 34 chromosomes. Among them, R2R3 was the primary MYB subclass, which was further divided into 21 subgroups with their Arabidopsis homologs. The evolution of the ArMYB family was also investigated, with the results revealing several R2R3-MYB subgroups with expanded membership in woody species. Quantitative real-time PCR analysis revealed that the ArMYB89 transcription level was significantly up-regulated in red leaves in contrast to green leaves. Here, we report on the isolation and characterization of ArMYB89 in red maple. Subcellular localization experiments indicated that ArMYB89 was localized on the nucleus. Further experiments revealed that ArMYB89 could interact with ArSGT1 in vitro and in vivo. The overexpression of ArMYB89 in tobacco might enhance the anthocyanin content of transgenic plants. In conclusion, our results contributed to the elucidation of a theoretical basis for the ArMYB gene family and provides a foundation for the further characterization of the biological roles of MYB genes in the regulation of Acer rubrum leaf color.
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http://dx.doi.org/10.1093/jxb/erab213DOI Listing
May 2021

Comparative transcriptome analysis of differentially expressed genes related to the physiological changes of yellow-green leaf mutant of maize.

PeerJ 2021 16;9:e10567. Epub 2021 Feb 16.

Tobacco Research Institute, Anhui Academy of Agricultural Sciences, Hefei, China.

Chlorophylls, green pigments in chloroplasts, are essential for photosynthesis. Reduction in chlorophyll content may result in retarded growth, dwarfism, and sterility. In this study, a yellow-green leaf mutant of maize, indicative of abnormity in chlorophyll content, was identified. The physiological parameters of this mutant were measured. Next, global gene expression of this mutant was determined using transcriptome analysis and compared to that of wild-type maize plants. The yellow-green leaf mutant of maize was found to contain lower contents of chlorophyll , chlorophyll and carotenoid compounds. It contained fewer active PSII centers and displayed lower values of original chlorophyll fluorescence parameters than the wild-type plants. The real-time fluorescence yield, the electron transport rate, and the net photosynthetic rate of the mutant plants showed reduction as well. In contrast, the maximum photochemical quantum yield of PSII of the mutant plants was similar to that of the wild-type plants. Comparative transcriptome analysis of the mutant plants and wild-type plants led to the identification of differentially expressed 1,122 genes, of which 536 genes were up-regulated and 586 genes down-regulated in the mutant. Five genes in the chlorophyll metabolism pathway, nine genes in the tricarboxylic acid cycle and seven genes related to the conversion of sucrose to starch displayed down-regulated expression. In contrast, genes encoding a photosystem II reaction center PsbP family protein and the PGR5-like protein 1A (PGRL1A) exhibited increased transcript abundance.
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http://dx.doi.org/10.7717/peerj.10567DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7894110PMC
February 2021

Functional analysis of ZmMADS1a reveals its role in regulating starch biosynthesis in maize endosperm.

Sci Rep 2019 03 1;9(1):3253. Epub 2019 Mar 1.

National Engineering Laboratory of Crop Stress Resistence, Anhui Agricultural University, Hefei, 230036, China.

MADS-box family proteins play an important role in grain formation and flower development; however, the molecular mechanisms by which transcription factors regulate the starch metabolism pathway are unclear in maize. Here, we report a transcription factor, ZmMADS1a, that controls starch biosynthesis in maize (Zea mays L.). We demonstrate the expression of ZmMADS1a in tassel, silk, and endosperm, and show that the protein is localized to the cell nucleus. Compared with the control, seeds of overexpressing ZmMADS1a increased starch content (especially amylose content), had smaller starch granules and altered chemical structure. Meanwhile, overexpression of ZmMADS1a resulted in increases in the contents of soluble sugars and reducing sugars in maize. ZmMADS1a plays a positive regulatory role in the starch biosynthesis pathway by up-regulating several starch biosynthesis related genes. We also show that ZmMADS1a has a similar adjustment mechanism of starch biosynthesis in rice. Collectively, our study suggests that ZmMADS1a functions as a positive regulator of starch biosynthesis by regulating the expression of key starch metabolism genes during seed development.
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http://dx.doi.org/10.1038/s41598-019-39612-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6397188PMC
March 2019

Comparative transcriptome analysis reveals differentially expressed genes related to the tissue-specific accumulation of anthocyanins in pericarp and aleurone layer for maize.

Sci Rep 2019 02 21;9(1):2485. Epub 2019 Feb 21.

Corn Research Center, Tobacco Research Institute, Anhui Academy of Agricultural Sciences, Hefei, 230031, P. R. China.

Purple corn is a rich source of anthocyanins. In the experiment, two anthocyanins-enriched purple corn lines Ha0414 and Ha6130 were identified. The anthocyanins were respectively accumulated in the pericarp of Ha0414 and the aleurone layer of Ha6130 with different composition and content. Transcriptome analysis of the two tissues in both lines identified 16 and 14 differentially expressed genes belonging to anthocyanin metabolism pathway in pericarp and the aleurone layer, individually. Of these genes, two genes encoding 2-oxoglutarate (2OG) and Fe (II)-dependent oxygenase superfamily proteins, and one gene annotated as UDP-glycosyltransferase superfamily protein exhibited increased transcript abundance in both the colored pericarp and aleurone layer. Otherwise, one gene annotated as flavonoid 3', 5'-hydroxylase, and another gene encoding flavonoid 3'-monooxygenase displayed increased transcript abundance in the aleurone layer of Ha6130. Moreover, 36 transcription factors were identified with increased transcript abundance in the pericarp of Ha0414, such as bHLH transcription factors, WRKY transcription factors, and HB transcription factors. And 79 transcription factors were isolated with an increased expression level in the aleurone layer of Ha6130, including MYB transcription factors, MYB-related transcription factors, and bHLH transcription factors. These genes expression may result in the tissue-specific accumulation of anthocyanins in pericarp and aleurone layer.
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http://dx.doi.org/10.1038/s41598-018-37697-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6384890PMC
February 2019
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