Publications by authors named "Ting-Ting Zhang"

318 Publications

Rapid tumor recurrence in a novel murine GBM surgical model is associated with Akt/PD-L1/vimentin signaling.

Biochem Biophys Res Commun 2021 Jun 30;569:1-9. Epub 2021 Jun 30.

Department of Pathophysiology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Key Laboratory of Ministry of Education for Neurological Disorders, Wuhan, 430030, Hubei, China. Electronic address:

Glioblastoma multiforme (GBM) is the most common primary malignant brain tumor without curable therapy. Surgical resection remains the first choice of patients with GBM but tumors relapse rapidly even combined with conventional chemoradiotherapy. The mechanism of GBM rapid recurrence is poorly understood, which is largely due to the lack of an appropriate animal model, thus heavily impedes the improvement of postoperative therapy. Here we established a highly reproducible mouse GBM surgical model by using the syngeneic G422-GBM cells, which faithfully recapitulates the features of rapid recurrence of human GBM after surgery. Implanting 2 × 10-5 × 10 of G422-GBM cells in mouse cerebral cortex caused death in all animal within 23 days, while surgery was an effective therapy but not curable. After complete removal of visible tumors on day 5-9 of tumor growth, the tumors recurred macroscopically within 5 days accompanied by increasing infiltrative cancer foci. Mechanistically, the rapid recurrence of resected tumors was positively correlated to early Akt activation, which subsequently upregulated PD-L1/Vimentin and promoted proliferation/migration of cancer cells. In addition, environmental astrocytic activation with strong PD-L1 signal was prominent. Taken together, we provided a novel GBM surgical recurrence model for preclinical studies and suggested complicated recurring mechanisms involving in strong oncogenic signaling as well as immune inhibitory signals from both GBM cells and their neighboring astrocytes.
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http://dx.doi.org/10.1016/j.bbrc.2021.06.072DOI Listing
June 2021

An Evaluation of the Tolerability and Feasibility of Combining 5-Amino-Levulinic Acid (5-ALA) with BCNU Wafers in the Surgical Management of Primary Glioblastoma.

Cancers (Basel) 2021 Jun 29;13(13). Epub 2021 Jun 29.

Cancer Research UK and University College London Cancer Trials Centre, London W1T 4TJ, UK.

Glioblastoma (GBM) is the commonest primary malignant brain tumour in adults and effective treatment options are limited. Combining local chemotherapy with enhanced surgical resection using 5-aminolevulinic acid (5-ALA) could improve outcomes. Here we assess the safety and feasibility of combining BCNU wafers with 5-ALA-guided surgery. We conducted a multicentre feasibility study of 5-ALA with BCNU wafers followed by standard-of-care chemoradiotherapy (chemoRT) in patients with suspected GBM. Patients judged suitable for radical resection were administered 5-ALA pre-operatively and BCNU wafers at the end resection. Post-operative treatment continued as per routine clinical practice. The primary objective was to establish if combining 5-ALA and BCNU wafers is safe without compromising patients from receiving standard chemoRT. Seventy-two patients were recruited, sixty-four (88.9%) received BCNU wafer implants, and fifty-nine (81.9%) patients remained eligible following formal histological diagnosis. Seven (11.9%) eligible patients suffered surgical complications but only two (3.4%) were not able to begin chemoRT, four (6.8%) additional patients did not begin chemoRT within 6 weeks of surgery due to surgical complications. Eleven (18.6%) patients did not begin chemoRT for other reasons (other toxicity ( = 3), death ( = 3), lost to follow-up/withdrew ( = 3), clinical decision ( = 1), poor performance status ( = 1)). Median progression-free survival was 8.7 months (95% CI: 6.4-9.8) and median overall survival was 14.7 months (95% CI: 11.7-16.8). Combining BCNU wafers with 5-ALA-guided surgery in newly diagnosed GBM patients is both feasible and tolerable in terms of surgical morbidity and overall toxicity. Any potential therapeutic benefit for the sequential use of 5-ALA and BCNU with chemoRT requires further investigation with improved local delivery technologies.
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http://dx.doi.org/10.3390/cancers13133241DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8267684PMC
June 2021

Platelet count as an independent risk factor for acute kidney injury induced by rhabdomyolysis.

Chin Med J (Engl) 2021 Jun 24. Epub 2021 Jun 24.

Department of Intensive Care Medicine, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, 230036, China.

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http://dx.doi.org/10.1097/CM9.0000000000001651DOI Listing
June 2021

Jasmonate signaling restricts root soluble sugar accumulation and drives root-fungus symbiosis loss at flowering by antagonizing gibberellin biosynthesis.

Plant Sci 2021 Aug 19;309:110940. Epub 2021 May 19.

Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal University, Nanjing, China. Electronic address:

Jasmonate restricts accumulation of constitutive and fungus-induced root soluble sugars at flowering stage, and thus reduces root beneficial fungal colonization, but little is known about how these are achieved. To determine whether jasmonate-mediated depletion of soluble sugars is the result of direct phytohormonal cross-talk or indirect induced defensive secondary metabolism, we first profiled soluble sugar and tryptophan (Trp)-derived defensive secondary metabolites in the roots of wild-type and jasmonate signaling-impaired Arabidopsis thaliana at flowering upon a beneficial fungus Phomopsis liquidambaris inoculation. Next, jasmonate and gibberellin signaling were manipulated to determine the relationship between jasmonate and gibberellin, and to quantify the effects of these phytohormones on fungal colonization degree, soluble sugar accumulation, Trp-derived secondary metabolites production, and sugar source-sink transport and metabolism. Gibberellin complementation increased Ph. liquidambaris colonization and rescued jasmonate-dependent root soluble sugar depletion and phloem sugar transport and root invertase activity without influencing jasmonate-induced Trp-derived secondary metabolites production at flowering. Furthermore, jasmonate signaling antagonized gibberellin biosynthesis in Ph. liquidambaris-inoculated roots. Our results suggest a phytohormonal antagonism model that jasmonate signaling restricts root soluble sugar accumulation through antagonizing gibberellin biosynthesis rather than through promoting Trp-derived secondary metabolites production and thus drives beneficial fungal colonization decline at flowering.
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http://dx.doi.org/10.1016/j.plantsci.2021.110940DOI Listing
August 2021

Silencing of transcription factor E93 inhibits adult morphogenesis and disrupts cuticle, wing and ovary development in Locusta migratoria.

Insect Sci 2021 Jun 11. Epub 2021 Jun 11.

Research Institute of Applied Biology, Shanxi University, Taiyuan, China.

Ecdysone-induced protein 93F (E93) plays important roles during the metamorphosis process in insects. In this study, a cDNA of the LmE93 gene was identified from the transcriptome of Locusta migratoria, which consists of the 3378-nucleotide open-reading frame (ORF) and encodes 1125 amino acids with helix-turn-helix (HTH) motifs. Reverse transcription quantitative polymerase chain reaction analysis revealed that LmE93 was highest expressed in ovary. The LmE93 expression level was markedly low from the 3rd to 4th instar nymphs, and greatly increased in 1-day-old 5th instar nymphs with a peak on middle nymphal days, then declined in the late nymphal days. Moreover, injected dsLmE93 into 4th and 5th instar nymphs greatly reduced LmE93 transcripts, respectively, and prevented the process of metamorphosis, causing supernumerary nymphal stages. Hematoxylin-eosin staining of the integument showed that the apolysis occurred in advance in 4th instar nymphs, and old cuticle degradation was decreased in dsLmE93-injected locusts of 5th instar nymphs. Smaller and no fully developed wings with reduced columns between the anterior and posterior regions were found in N6 and N7 supernumerary nymphs. In addition, the development of the ovary in dsLmE93-injected locusts was severely blocked, the yolk was almost not formed and there was no development of ovarioles. The results indicated that LmE93 play key roles in the metamorphosis, cuticle, wing and ovarian development of locusts.
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http://dx.doi.org/10.1111/1744-7917.12924DOI Listing
June 2021

The complete mitochondrial genome of Decne. (Asteraceae, Falc.).

Mitochondrial DNA B Resour 2021 May 19;6(6):1691-1693. Epub 2021 May 19.

School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, China.

The complete mitochondrial genome of was sequenced for the first time. The mitochondrial genome length was 320,439 bp, with 45.05% GC contents. There were 67 genes annotated, including 31 known protein-coding genes, 25 tRNAs, and six rRNAs. The maximum likelihood method was used to establish the phylogenetic tree of 37 species. Results have shown that and were sister groups. It reveals the genetic relationship between different species and provides a theoretical basis for the establishment of a classification system.
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http://dx.doi.org/10.1080/23802359.2021.1914524DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8143591PMC
May 2021

Novel Structural Variation and Evolutionary Characteristics of Chloroplast tRNA in Plants.

Genes (Basel) 2021 May 27;12(6). Epub 2021 May 27.

Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi'an 710069, China.

Cotton is one of the most important fiber and oil crops in the world. Chloroplast genomes harbor their own genetic materials and are considered to be highly conserved. Transfer RNAs (tRNAs) act as "bridges" in protein synthesis by carrying amino acids. Currently, the variation and evolutionary characteristics of tRNAs in the cotton chloroplast genome are poorly understood. Here, we analyzed the structural variation and evolution of chloroplast tRNA (cp tRNA) based on eight diploid and two allotetraploid cotton species. We also investigated the nucleotide evolution of chloroplast genomes in cotton species. We found that cp tRNAs in cotton encoded 36 or 37 tRNAs, and 28 or 29 anti-codon types with lengths ranging from 60 to 93 nucleotides. Cotton chloroplast tRNA sequences possessed specific conservation and, in particular, the Ψ-loop contained the conserved U-U-C-X3-U. The cp tRNAs of L. contained introns, and cp tRNA contained the anti-codon (C-A-U), which was generally the anti-codon of tRNA. The transition and transversion analyses showed that cp tRNAs in cotton species were iso-acceptor specific and had undergone unequal rates of evolution. The intergenic region was more variable than coding regions, and non-synonymous mutations have been fixed in cotton cp genomes. On the other hand, phylogeny analyses indicated that cp tRNAs of cotton were derived from several inferred ancestors with greater gene duplications. This study provides new insights into the structural variation and evolution of chloroplast tRNAs in cotton plants. Our findings could contribute to understanding the detailed characteristics and evolutionary variation of the tRNA family.
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http://dx.doi.org/10.3390/genes12060822DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8228828PMC
May 2021

Recent progress towards the transition-metal-catalyzed Nazarov cyclization of alkynes via metal carbenes.

Org Biomol Chem 2021 Jun 1;19(24):5274-5283. Epub 2021 Jun 1.

College of Sciences and College of Forestry, Henan Agricultural University, Zhengzhou, Henan 450002, China.

In recent years, transition-metal-catalyzed tandem cyclization reactions of alkynes, especially those involving a metal carbene intermediate, have received worthwhile interest, as this type of reaction does not require the use of risky and potentially explosive diazo compounds as starting materials for carbene generation. A significant and general strategy for the stereospecific synthesis of 5-membered cycles is Nazarov cyclization based on the 4π-conrotatory electrocyclization of a conjugated pentadienyl cation to afford a cyclopentenyl cation. In this review, we introduce an overview of recent advances in the transition-metal-catalyzed Nazarov cyclization of alkynes via a metal carbene intermediate, and categorize these reactions according to the structure of the metal carbene. Our aim is to accelerate advancements in this enchanting area of research.
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http://dx.doi.org/10.1039/d1ob00744kDOI Listing
June 2021

Living-Cell MicroRNA Imaging with Self-Assembling Fragments of Fluorescent Protein-Mimic RNA Aptamer.

ACS Sens 2021 06 24;6(6):2339-2347. Epub 2021 May 24.

State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.

As the cellular roles of RNA abundance continue to increase, there is an urgent need for the corresponding tools to elucidate native RNA functions and dynamics, especially those of short, low-abundance RNAs in live cells. Fluorescent RNA aptamers provide a useful strategy to create the RNA tag and biosensor devices. Corn, which binds with 3,5-difluoro-4-hydroxybenzylidene-imidazolinone-2-oxime (DFHO), is a good candidate for the RNA tag because of its enhanced photostability and red-shifted spectrum. Herein, we report for the first time the utilization of Corn as a split aptamer system, combined with RNA-initiated fluorescence complementation (RIFC), for monitoring RNA self-assembly and sensing microRNA. In this platform, the 28-nt Corn was divided into two nonfunctional halves (named probe I and probe II), and an additional target RNA recognition and stem part was introduced in each probe. The target RNA can trigger the self-assembly reconstitution of the Corn's G-quadruplex scaffold for DFHO binding and turn-on fluorescence. These probes can be transfected stably into mammalian cells and deliver the light-up fluorescent response to microRNA-21 (miR-21). Significantly, the probes have good photostability, with minimal fluorescence loss after continuous irradiation, and can be used for imaging of miR-21 in living mammalian cells. The proposed method is universal and could be applied to the sensing of other tumor-associated RNAs, including messenger RNA and noncoding RNA, as well as for monitoring RNA/RNA interactions. The Corn-based splitting aptamers show promising potential in the real-time visualization and mechanistic analysis of nucleic acids.
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http://dx.doi.org/10.1021/acssensors.1c00453DOI Listing
June 2021

[Study on chemical constituents from fruiting bodies of Ganoderma calidophilum].

Zhongguo Zhong Yao Za Zhi 2021 Apr;46(7):1783-1789

Haikou Key Laboratory for Research and Utilization of Tropical Natural Products, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences Haikou 571101, China.

Chemical constituents were isolated and purified from fruiting bodies of Ganoderma calidophilum by various column chromatographic techniques, and their chemical structures were identified through combined analysis of physicochemical properties and spectral data. As a result, 11 compounds were isolated and identified as(24E)-lanosta-8,24-dien-3,11-dione-26-al(1), ganoderone A(2), 3-oxo-15α-acetoxy-lanosta-7,9(11), 24-trien-26-oleic acid(3),(23E)-27-nor-lanosta-8,23-diene-3,7,25-trione(4), ganodecanone B(5), ganoderic aldehyde A(6), 11β-hydroxy-lucidadiol(7), 3,4-dihydroxyacetophenone(8), methyl gentiate(9), ganoleucin C(10), ganotheaecolumol H(11). Among them, compound 1 is a new triterpenoid. The cytotoxic activities of all of the compounds against tumor cell lines were evaluated. The results showed that compounds 1, 3, 4 and 6 showed cytotoxic activity against BEL-7402, with IC_(50) values of 26.55, 11.35, 23.23, 18.66 μmol·L~(-1); compounds 1 and 3-6 showed cytotoxic activity against K562, with IC_(50) values of 5.79, 22.16, 12.16, 35.32, and 5.59 μmol·L~(-1), and compound 4 showed cytotoxic activity against A549, with IC_(50) value of 42.50 μmol·L~(-1).
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20201223.602DOI Listing
April 2021

A novel 3-D lead-iodide polymer based on the linkage of rare binuclear [PbI] cations and anionic bis(pyrazinyl)-trizole bridges.

Dalton Trans 2021 Apr 25;50(13):4486-4489. Epub 2021 Mar 25.

School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, P.R. China.

A novel 3-D lead-iodide polymer [PbI(μ-bpt-z)] (1, Hbpt-z = 3,5-bis(pyrazinyl)-1,2,4-trizole) has been hydrothermally prepared and structurally characterized. 1 offers the first example of a 3-D lead-iodide polymer built up from the linkage of rare binuclear [PbI] cations and anionic bis(pyrazinyl)-trizole bridges. 1 exhibits photoluminescent and photocurrent response properties, and its density functional theory calculation was also studied.
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http://dx.doi.org/10.1039/d1dt00471aDOI Listing
April 2021

The complete mitochondrial genome of Rchb. (Ranales, Ranunculaceae).

Mitochondrial DNA B Resour 2021 Mar 11;6(3):779-781. Epub 2021 Mar 11.

School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, China.

Rchb. is a medicinal plant in the Ranunculaceae family. In this study, we report the first complete mitochondrial genome of . The total length of the mitochondrial genome of is 440,720 bp and the GC content of 46.85%. The mitochondrial genome contained 37 protein-coding genes, 29 tRNAs, and three rRNAs. These data will provide the basis for the systematic evolutionary analysis of Ranunculaceae.
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http://dx.doi.org/10.1080/23802359.2021.1882894DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7954411PMC
March 2021

Faciogenital Dysplasia 5 supports cancer stem cell traits in basal-like breast cancer by enhancing EGFR stability.

Sci Transl Med 2021 03;13(586)

Immunology and Cancer Pharmacology Group, State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.

Most basal-like breast cancers (BLBCs) are triple-negative breast cancers (TNBCs), which have the worst prognosis and distant metastasis-free survival among breast cancer subtypes. Now, no targeted therapies are available for patients with BLBC due to the lack of reliable and effective molecular targets. Here, we performed the BLBC tissue microarray-based immunohistochemical analysis and showed that Faciogenital Dysplasia 5 (FGD5) abundance is associated with poor prognosis in BLBCs. deletion decreased the proliferation, invasion, and tumorsphere formation capacity of BLBC cells. Furthermore, genetic inhibition of in mouse mammary epithelial cells attenuated BLBC initiation and progression by reducing the self-renewal ability of tumor-initiating cells. In addition, FGD5 abundance was positively correlated with the abundance of epidermal growth factor receptor (EGFR) in BLBCs. ablation decreased EGFR abundance by reducing EGFR stability in TNBC cells in 2D and 3D culture conditions. Mechanistically, FGD5 binds to EGFR and interferes with basal EGFR ubiquitination and degradation induced by the E3 ligase ITCH. Impaired EGFR degradation caused BLBC cell proliferation and promoted invasive properties and self-renewal. To verify the role of the FGD5-EGFR interaction in the regulation of EGFR stability, we screened a cell-penetrating α-helical peptide PER3 binding with FGD5 to disrupt the interaction. Treatment of BLBC patient-derived xenograft-bearing mice with the peptide PER3 disrupting the FGD5-EGFR interaction either with or without chemotherapy reduced BLBC progression. Our study identified FGD5 as a positive modulator of tumor-initiating cells and suggests a potential therapeutic option for the BLBC subtype of breast cancer.
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http://dx.doi.org/10.1126/scitranslmed.abb2914DOI Listing
March 2021

Two Organic Hybrid Iodoplumbates Directed by a Bifunctional Bis(pyrazinyl)triazole.

Inorg Chem 2021 Apr 23;60(7):5362-5366. Epub 2021 Mar 23.

Chongqing Key Laboratory of Inorganic Functional Materials, College of Chemistry, Chongqing Normal University, Chongqing 401331, People's Republic of China.

The two novel organic hybrid iodoplumbates [Hhbpt][Hhbpt][Hhbpt][PbI]·9HO (, hbpt = 1-3,5- bis(pyrazinyl)-1,2,4-triazole) and [PbI(bpt)(HO)(I·1/2I)] () were synthesized under hydrothermal conditions. contains a new type of pentanuclear cluster [PbI] anion, while comprises an unprecedented 2-D polyiodoplumbate layer. Both and are potential semiconductors with narrow absorption edges of 1.98 eV for and 1.38 eV for . also shows photocurrent response and photoluminescent properties.
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http://dx.doi.org/10.1021/acs.inorgchem.1c00330DOI Listing
April 2021

Controlled ovarian stimulation should not be preferred for male infertility treated with intrauterine insemination: a retrospective study.

Reprod Biol Endocrinol 2021 Mar 19;19(1):45. Epub 2021 Mar 19.

Department of Gynecology and Obstetrics, Centre for Reproductive Medicine, Zhongshan City People's Hospital, No. 2, Sunwen East Road, Shiqi District, Zhongshan, 528400, Guangdong Province, China.

Background: Some studies have stated that intrauterine insemination (IUI) with controlled ovarian stimulation (COS) might increase the pregnancy rate, while others suggest that IUI in the natural cycle (NC) should be the first line of treatment. It remains unclear whether it is necessary to use COS at the same time when IUI is applied to treat isolated male factor infertility. Thus, we aimed to investigate efficacy of IUI with COS for isolated male factor infertility.

Methods: A total of 601 IUI cycles from 307 couples who sought medical care for isolated male factor infertility between January 2010 and February 2020 were divided into two groups: NC-IUI and COS-IUI. The COS-IUI group was further divided into two subgroups according to the number of pre-ovulatory follicles on the day of HCG: cycles with monofollicular development (one follicle group) and cycles with at least two pre-ovulatory follicles (≥ 2 follicles group). The IUI outcomes, including clinical pregnancy, live birth, spontaneous abortion, ectopic pregnancy, and multiple pregnancy rates were compared.

Results: The clinical pregnancy, live birth, spontaneous abortion, and ectopic pregnancy rates were comparable between the NC-IUI and COS-IUI group. Similar results were also observed among the NC-IUI, one follicle, and ≥ 2 follicles groups. However, with respect to the multiple pregnancy rate, a trend toward higher multiple pregnancy rate was observed in the COS-IUI group compared to the NC-IUI group (8.7% vs. 0, P = 0.091), and a significant difference was found between the NC-IUI and ≥ 2 follicles group (0 vs. 16.7%, P = 0.033).

Conclusion: In COS cycles, especially in those with at least two pre-ovulatory follicles, the multiple pregnancy rate increased without a substantial gain in overall pregnancy rate; thus, COS should not be preferred in IUI for isolated male factor infertility. If COS is required, one stimulated follicle and one healthy baby should be the goal considering the safety of both mothers and foetuses.
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http://dx.doi.org/10.1186/s12958-021-00730-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7977560PMC
March 2021

Bowel Wall Visualization Using MR Enterography in Relationship to Bowel Lumen Contents and Patient Demographics.

J Magn Reson Imaging 2021 Mar 4. Epub 2021 Mar 4.

Department of Radiology and Biomedical Imaging, UCSF Medical Center, San Francisco, California, USA.

Background: MR enterography (MRE) is generally performed without bowel preparation, but the frequency and extent by which bowel contents affect bowel wall visualization are poorly described in the literature.

Purpose: To evaluate MRE bowel wall visualization quality relative to bowel lumen contents and patient demographics.

Study Type: Retrospective, single-center.

Population: One hundred and four consecutive patients (mean age 29 years, range 5-76 years) without prior bowel resection who had undergone MRE.

Field Strength/sequence: 3 T (N = 87) or 1.5 T (N = 17)/T2-weighted single-shot spin echo (T2WI) and fat-saturated T1-weighted gradient echo (T1WI) without and with gadolinium.

Assessment: For the proximal and distal jejunum and ileum and colon, three readers independently categorized bowel lumen signal (water = bright T2 dark T1, T1-bright, or air = dark T2 and T1 signal) and scored distension (0 = poor, 1 = moderate, 2 = well) and wall enhancement (0 = unclear, 1 = perceptible, 2 = clear) based upon gadolinium enhanced T1WI for the 104 MRE exams). The bowel visualization score was the sum of the wall enhancement and distension scores and was considered adequate if ≥3.

Statistical Tests: Wilcoxon signed-rank test.

Results: The bowel lumen content was water signal in 93% (97/104 scans), 92% (95/104), 98% (102/104), and 93% (92/104) of the proximal and distal jejunum and proximal and distal ileum, respectively, but only in 12.5% (13/104) of the colon. There was adequate bowel visualization of 53.8%, 77.8%, 84.6%, 90.4% of the proximal and distal jejunum and proximal and distal ileum, respectively, but only 19.2% of the colon. In children (age < 18 years), the visualization score of the ileum was lower when the adjacent colon contained air (2.4 ± 0.97) compared to water (3.75 ± 0.29, P < 0.05) or T1-bright material (3.21 ± 0.82, P < 0.05).

Data Conclusion: Without bowel preparation, colon wall visualization was often unsatisfactory at MRE, and air-filled colon also degraded small bowel visualization, particularly in children.

Level Of Evidence: 4 TECHNICAL EFFICACY: Stage 1.
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http://dx.doi.org/10.1002/jmri.27589DOI Listing
March 2021

Characteristics of Halloween genes and RNA interference-mediated functional analysis of LmCYP307a2 in Locusta migratoria.

Insect Sci 2021 Feb 25. Epub 2021 Feb 25.

Research Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi, 030006, China.

Halloween genes are involved in the biosynthesis of the molting hormone, which plays a key role in insect ecdysis, development, metamorphosis, and reproduction. Our previous work identified five Halloween genes from Locusta migratoria, but their functions are currently unknown. In this study, the sequences of these five Halloween genes were analyzed and characterized. LmCYP307a2, LmCYP306a1, LmCYP302a1, and LmCYP315a1 were primarily expressed in the prothoracic glands, while LmCYP314a1 was universally expressed in peripheral tissues, especially in the ovaries and Malpighian tubules. All five Halloween genes were mainly expressed from the 5th to the 7th d in 5th-instar nymphs. RNA interference (RNAi) silencing of LmCYP307a2 resulted in severe molting delays and molting failure, which could be rescued by supplementary 20-hydroxyecdysone. A hematoxylin and eosin staining analysis suggested that the RNAi of LmCYP307a2 inhibited the ecdysis process by inhibiting the apolysis and degradation of the old cuticle, and by promoting the synthesis of a new cuticle. Quantitative reverse transcription polymerase chain reaction results showed that the expressions of LmE74, LmCht5, and LmCht10 were dramatically down-regulated, while that of LmChsI was substantially up-regulated, after knockdown of LmCYP307a2. The results suggest that LmCYP307a2 is related to the molt process via regulation of chitin synthesis and degradation.
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http://dx.doi.org/10.1111/1744-7917.12907DOI Listing
February 2021

The complete mitochondrial genome of Fisch. (Fabales, Leguminosae).

Mitochondrial DNA B Resour 2021 Feb 9;6(2):475-477. Epub 2021 Feb 9.

School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, China.

The complete mitochondrial genome of an important medicinal plant Fisch. is reported for the first time. The mitochondrial genome sequence of was 463,869 bp in length and had a GC content of 45.19%. The genome contained 40 protein-coding genes (PCGs), 30 transfer RNAs (tRNAs), and three ribosomal RNAs (rRNAs). The phylogenetic tree was built based on 25 plants, using the maximum-likelihood method. These data will provide certain help to determine the taxonomic status of .
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http://dx.doi.org/10.1080/23802359.2021.1872432DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7889147PMC
February 2021

Phylogeny and evolution of chloroplast tRNAs in Adoxaceae.

Ecol Evol 2021 Feb 6;11(3):1294-1309. Epub 2021 Jan 6.

Shaanxi Key Laboratory for Animal Conservation Key Laboratory of Resource Biology and Biotechnology in Western China Ministry of Education College of Life Sciences Northwest University Xi'an China.

Chloroplasts are semiautonomous organelles found in photosynthetic plants. The major functions of chloroplasts include photosynthesis and carbon fixation, which are mainly regulated by its circular genomes. In the highly conserved chloroplast genome, the chloroplast transfer RNA genes (cp tRNA) play important roles in protein translation within chloroplasts. However, the evolution of cp tRNAs remains unclear. Thus, in the present study, we investigated the evolutionary characteristics of chloroplast tRNAs in five Adoxaceae species using 185 tRNA gene sequences. In total, 37 tRNAs encoding 28 anticodons are found in the chloroplast genome in Adoxaceae species. Some consensus sequences are found within the Ψ-stem and anticodon loop of the tRNAs. Some putative novel structures were also identified, including a new stem located in the variable region of tRNA in a similar manner to the anticodon stem. Furthermore, phylogenetic and evolutionary analyses indicated that synonymous tRNAs may have evolved from multiple ancestors and frequent tRNA duplications during the evolutionary process may have been primarily caused by positive selection and adaptive evolution. The transition and transversion rates are uneven among different tRNA isotypes. For all tRNAs, the transition rate is greater with a transition/transversion bias of 3.13. Phylogenetic analysis of cp tRNA suggested that the type I introns in different taxa (including eukaryote organisms and cyanobacteria) share the conserved sequences "U-U-x2-C" and "U-x-G-x2-T," thereby indicating the diverse cyanobacterial origins of organelles. This detailed study of cp tRNAs in Adoxaceae may facilitate further investigations of the evolution, phylogeny, structure, and related functions of chloroplast tRNAs.
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http://dx.doi.org/10.1002/ece3.7133DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7863635PMC
February 2021

Ternary Cocrystals with Large Soft Cavities: A 1,4-diiodotetrafluorobenzene (DITFB)⋅4-Biphenylpyridine N-oxide (BPNO) Host Assembled by Inclusion of Planar Aromatic Guests.

Chempluschem 2021 Feb;86(2):252-258

School of Chemistry and Chemical Engineering, Shanxi Datong University, Datong, Shanxi, 037009, P. R. China.

A large soft-cavity host composed of 1,4-diiodotetrafluorobenzene (DITFB) and 4-biphenylpyridine N-oxide (BPNO) is assembled under the mediation of a planar aromatic guest molecule (pyrene or perylene) through C-I⋅⋅⋅ O-N halogen bonds and π-hole⋅⋅⋅π bonds. Single-crystal X-ray diffraction reveals that guest molecules can be completely encapsulated in the four-layer host cavity to assemble ternary host-guest cocrystals; namely, [email protected] ⋅ BPNO and [email protected] ⋅ BPNO. The luminescence of these ternary cocrystals originates from their discrete guest molecules, which exhibit pure-blue and yellow emissions, respectively, that are localized at 425 nm and in the range of 485 to 578 nm, respectively. In addition, the contribution of different fragments to the stabilization of the crystal structure is estimated by computational chemistry. These cocrystals have significant potential for use in optical applications or materials, such as photonics or organic light-emitting diodes, respectively, that require to avoid the aggregation between luminophores.
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http://dx.doi.org/10.1002/cplu.202000779DOI Listing
February 2021

[Comparison of two types of T-shaped coronectomy applied in extraction of impacted mandibular third molars].

Shanghai Kou Qiang Yi Xue 2020 Oct;29(5):550-553

Department of Oral and Maxillofacial Surgery, Stomatological Hospital of Tianjin Medical University. Tianjin 300070, China.

Purpose: To compare the clinical efficiency of two types of T-shaped coronectomy applied in extraction of impacted mandibular third molars. METHODS:One hundred and twenty patients meeting with the criteria were randomly divided into 2 groups. Two types of T-shaped coronectomy were applied in extraction of impacted mandibular third molars. Indexes including operation time, intraoperative anxiety, postoperative pain and limitation of mouth opening were recorded and analyzed with SPSS 22.0 software package.

Results: Patients with transverse method experienced more time in operation [(17.41±3.72) vs (15.22±2.53) min], with less intraoperative anxiety (P<0.05). There was no significant difference in postoperative pain and limitation of mouth opening(P>0.05) between two methods.

Conclusions: Transverse T-shaped method can alleviate intraoperative anxiety, while longitudinal method is more conducive to shortening operation time.
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October 2020

The complete mitochondrial genome of (celastraceae,  L.).

Mitochondrial DNA B Resour 2021 Jan 16;6(1):182-184. Epub 2021 Jan 16.

School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, China.

The complete mitochondrial genome of medicinal plant, , was sequenced for the first time. The genome sequence is 1,045,106 bp in length (GenBank accession number MW009108), with 44.98% GC contents. There are 72 genes in the genome, including 41 known protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), and three ribosomal RNAs (rRNAs). The phylogenetic trees of 28 species are constructed using the maximum-likelihood method. The information will provide references for phylogenetic research.
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http://dx.doi.org/10.1080/23802359.2020.1860702DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7832542PMC
January 2021

Copper(I)-Catalyzed Enyne Oxidation/Cyclopropanation: Divergent and Enantioselective Synthesis of Cyclopropanes.

Org Lett 2021 02 2;23(4):1285-1290. Epub 2021 Feb 2.

College of Sciences, Henan Agricultural University, Zhengzhou, Henan 450002, China.

An efficient copper(I)-catalyzed enyne oxidation/cyclopropanation for the modular synthesis of cyclopropane derivatives is described, which represents the first non-noble metal-catalyzed enynes oxidation/cyclopropanation by the in situ generated α-oxo copper carbenes. This protocol allows the assembly of valuable cyclopropane-γ-lactams in generally good to excellent yields with excellent diastereoselectivity. More significantly, the enantioselective version of enyne oxidation/cyclopropanation has been disclosed with chiral copper catalysts.
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http://dx.doi.org/10.1021/acs.orglett.0c04268DOI Listing
February 2021

NIN-like protein 7 promotes nitrate-mediated lateral root development by activating transcription of TRYPTOPHAN AMINOTRANSFERASE RELATED 2.

Plant Sci 2021 Feb 25;303:110771. Epub 2020 Nov 25.

State Key Laboratory of Crop Biology, Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An, 271018, Shandong, China. Electronic address:

Nitrate is essential for plant growth and development. When nitrate availability is low, plants produce more lateral roots (LRs) to seek nitrate from the soil. In this study, by DNA electrophoretic mobility shift and luciferase assays, it was showed that NIN-like protein 7 (NLP7) transcription factor activated expression of TAR2 by directly binding to its promoter. Finally, through genetic analysis, it was speculated that NLP7 regulated LR development through TAR2. In conclusion, NLP7 binds to the TAR2 promoter and activates TAR2 expression, thereby promoting nitrate-dependent LR development.
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http://dx.doi.org/10.1016/j.plantsci.2020.110771DOI Listing
February 2021

The complete chloroplast genome of (Umbelliferae, L.).

Mitochondrial DNA B Resour 2020 Jul 28;5(3):3025-3027. Epub 2020 Jul 28.

School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, China.

is an important medicinal plant. Chloroplast genome information is helpful for the development of molecular markers and the study of plant phylogeny. In this study, we report the complete chloroplast genome sequence of . The genome sequence is 148,493 bp in size (GenBank accession number MN652885), with 37.25% GC contents. There are 127 genes in the genome, including 83 known protein-coding genes (PCGs), 36 transfer RNAs (tRNAs), and 8 ribosomal RNAs (rRNAs). The maximum-likelihood method are used to construct phylogenetic tree of 32 species. These data will provide certain theoretical basis for plant genetics research.
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http://dx.doi.org/10.1080/23802359.2020.1797560DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7781892PMC
July 2020

A fixed nitrous oxide/oxygen mixture as an analgesic for patients with postherpetic neuralgia: study protocol for a randomized controlled trial.

Trials 2021 Jan 6;22(1):29. Epub 2021 Jan 6.

School of Nursing, Ningxia Medical University, 1160 Sheng Li Street, Yinchuan, 750004, China.

Background: The pain management of postherpetic neuralgia (PHN) remains a major challenge, with no immediate relief. Nitrous oxide/oxygen mixture has the advantages of quick analgesic effect and well-tolerated. The purpose of this study is to investigate the analgesic effect and safety of nitrous oxide/oxygen mixture in patients with PHN.

Methods/design: This study is a single-center, two-group (1:1), randomized, placebo-controlled, double-blind clinical trial. A total of 42 patients with postherpetic neuralgia will be recruited and randomly divided into the intervention group and the control group. The control group will receive routine treatment plus oxygen, and the intervention group will receive routine treatment plus nitrous oxide/oxygen mixture. Data collectors, patients, and clinicians are all blind to the therapy. The outcomes of each group will be monitored at baseline (T0), 5 min (T1), and 15 min (T2) after the start of the therapy and at 5 min after the end of the therapy (T3). The primary outcome measure will be the pain intensity. Secondary outcomes included physiological parameters, adverse effects, patients' acceptance of analgesia, and satisfaction from patients.

Discussion: Previous studies have shown that nitrous oxide/oxygen mixture can effectively relieve cancer patients with breakthrough pain. This study will explore the analgesic effect of oxide/oxygen mixture on PHN. If beneficial to patients with PHN, it will contribute to the pain management of PHN.

Trial Registration: Chinese Clinical Trial Register ChiCTR1900023730 . Registered on 9 June 2019.
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http://dx.doi.org/10.1186/s13063-020-04960-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7787626PMC
January 2021

The apple MdCOP1-interacting protein 1 negatively regulates hypocotyl elongation and anthocyanin biosynthesis.

BMC Plant Biol 2021 Jan 6;21(1):15. Epub 2021 Jan 6.

State Key Laboratory of Crop Biology, Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An, 271018, Shandong, China.

Background: In plants, CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1) is a key negative regulator in photoperiod response. However, the biological function of COP1-interacting protein 1 (CIP1) and the regulatory mechanism of the CIP1-COP1 interaction are not fully understood.

Results: Here, we identified the apple MdCIP1 gene based on the Arabidopsis AtCIP1 gene. Expression pattern analysis showed that MdCIP1 was constitutively expressed in various tissues of apple, and responded to stress and hormone signals at the transcriptional level. Ectopic expression of MdCIP1 complemented the phenotypes of the Arabidopsis cip1 mutant, and MdCIP1 inhibited anthocyanin biosynthesis in apple calli. In addition, the biochemical assay demonstrated that MdCIP1 could interact with MdCOP1 protein by their coiled-coil domain, and MdCIP1-OX/cop1-4 had a similar phenotype in photomorphogenesis with the cop1-4 mutant, suggesting that COP1 is epistatic to CIP1. Furthermore, the transient transformation assay indicated that MdCIP1 repressed anthocyanin biosynthesis in an MdCOP1-mediated pathway.

Conclusion: Take together, this study finds that MdCIP1 acts as a repressor in regulating hypocotyl elongation and anthocyanin biosynthesis through MdCOP1 in apple.
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http://dx.doi.org/10.1186/s12870-020-02789-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7789773PMC
January 2021

Evolutionary history of endangered and relict tree species in response to geological and climatic events in the Qinling Mountains and adjacent areas.

Ecol Evol 2020 Dec 11;10(24):14052-14066. Epub 2020 Nov 11.

Key Laboratory of Resource Biology and Biotechnology in Western China Ministry of Education College of Life Sciences Northwest University Xi'an China.

Geological and climatic events are considered to profoundly affect the evolution and lineage divergence of plant species. However, the evolutionary histories of tree species that have responded to past geological and climate oscillations in central China's mountainous areas remain mostly unknown. In this study, we assessed the evolutionary history of the endangered and relict tree species in the Qinling Mountains (QM) and adjacent areas in East Asia based on variations in the complete chloroplast genomes (cpDNA) and reduced-genomic scale single nucleotide polymorphisms (SNPs). Population structure and phylogenetic analysis based on the cpDNA variations suggested that could be divided into two intraspecific genetic lineages in the eastern and western sides of the QM (EQM and WQM, respectively) in East Asia. Molecular dating suggested that the intraspecific divergence of occurred approximately 39.2 million years ago during the later Paleogene. It was significantly correlated with the orogeny of the QM, where the formation of this significant geographic barrier in the region may have led to the divergence of independent lineages. Bayesian clustering and demographic analysis showed that intraspecific gene flow was restricted between the EQM and WQM lineages. Isolation-with-migration analysis indicated that the two genetic lineages experienced significant demographic expansions after the Pleistocene ice ages. However, the genetic admixture was determined in some populations between the two lineages by the large scale of SNP variations due to DNA incompatibility, the large significant population size, and rapid gene flow of nuclear DNA markers. Our results suggest that two different conservation and management units should be constructed for in the EQM and WQM areas. These findings provide novel insights into the unprecedented effects of tectonic changes and climatic oscillations on lineage divergence and plant population evolution in the QM and adjacent areas in East Asia.
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http://dx.doi.org/10.1002/ece3.6996DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7771168PMC
December 2020

Validation of the Chinese Version of the Quality of Dying and Death Questionnaire for Family Members of ICU Patients.

J Pain Symptom Manage 2021 Jan 1. Epub 2021 Jan 1.

Department of Nursing, Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, China. Electronic address:

Context: The quality of end-of-life care services directly affects the end-of-life quality of life of patients and their families. At present, there are no standard tools in China for assessing the quality of dying and death (QODD) of critical intensive care unit (ICU) patients.

Objectives: This study aimed to introduce the Chinese version of the QODD questionnaire for family members of ICU patients, after transcultural adaptation and validation, to provide an effective instrument for assessing the quality of end-of-life care of ICU patients in China, fill the gap in the evaluation of the quality of end-of-life care of critical ICU patients in China, and offer a theoretical basis and practical guidance during purposeful intervention.

Methods: This study involved the main adult caregivers or principal family members of 149 dying critically ill patients. The original QODD scale was translated using the double forward and backward method. Nine cultural adaptation experts adapted the Chinese version of the QODD scale for completion by family members of ICU patients. Then, we carried out content validity, structural validity, internal consistency, confirmatory factors, and item correlation analysis of the modified scale.

Results: The Chinese version of the QODD for family members of ICU patients was developed after some items were deleted or modified. The content validity index was 0.93, indicating that all items were correlated with the measurement of death quality. The Kaiser-Meyer-Olkin value was 0.797, suggesting that the correlations between items were high. The Cronbach's α was 0.865, indicating good internal consistency. In confirmatory factor analysis, the fit indices were χ = 207.327, non-normed fit index = 0.916, root mean square error of approximation = 0.033, and comparative fit index = 0.93, indicating a good fit of the five-factor model of the Chinese version of the QODD questionnaire for family members of ICU patients.

Conclusion: The Chinese version of the QODD questionnaire for family members of ICU patients is a reliable and effective instrument for evaluating the quality of death among patients who die in the ICU and can be applied to clinical practice and research.
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http://dx.doi.org/10.1016/j.jpainsymman.2020.12.022DOI Listing
January 2021

Biobinder Nanocoating for Upgrading the Assembling Structures of High-Capacity Composite Electrodes with a Robust Polymeric Artificial Solid Electrolyte Interphase.

ACS Appl Mater Interfaces 2020 Dec 17;12(52):58201-58211. Epub 2020 Dec 17.

College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, Sichuan, China.

The success of next-generation lithium-ion batteries (LIBs) fundamentally depends on the rational design of not only the microstructure of an individual component but the component assembling structures on the electrode level. However, building advanced assembling structures for especially high-capacity electrodes is an urgent but a challenging task due to the lacking of in-depth understanding and effective strategies. Here, we propose a functional nanocoating biobinder using the well-known poly(lactic acid) to address the above need. It is found that the composite electrodes with this nanocoating biobinder are upgraded with uniform and robust assembling structures, including the electron-transportation network, ion-transportation network, and interfaces. Importantly, the nanocoating finally works as a new type of polymeric artificial cathode electrolyte interphase (poly-CEI) to protect the active particles. Therefore, a remarkable improvement in the electrochemical performance has been achieved for high-capacity electrodes (LiFePO, lithium nickel cobalt manganite (NCM), and lithium nickel cobalt aluminum acid (NCA)). In particular, the LFP cathode can deliver a high discharge capacity of 74.6 mA h g at 10C and a high capacity retention of 95.5% even after 850 cycles at 2C. For NCA and NCM cathodes, the cycling stability is dramatically improved due to the protection by the poly-CEI. In short, this study may reshape the essential roles of a binder in composite electrodes by highlighting its critical link to assembling structures.
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http://dx.doi.org/10.1021/acsami.0c16589DOI Listing
December 2020