Publications by authors named "Ting Zhang"

3,362 Publications

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Umami peptides screened based on peptidomics and virtual screening from Ruditapes philippinarum and Mactra veneriformis clams.

Food Chem 2022 Jun 16;394:133504. Epub 2022 Jun 16.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315211, Zhejiang, China. Electronic address:

The existing technology used for screening umami peptides is time-consuming and labor-intensive, making it difficult to meet the requirements of rapid screening of peptides. In this study, a high-throughput screening method for umami peptides was established based on peptidomics and virtual screening including the mass spectrometry, iUmami-SCM, PeptideRanker, and T1R1/T1R3 receptor. Subsequently, they were characterized and validated using sensory evaluation and electronic tongue. Results showed that 18 potential umami peptides were screened from two clams. Among them, 16 peptides had umami characteristics with thresholds range 0.123-1.481 mmol/L, and the accuracy of the screening method was about 88.9%. Additionally, active sites such as Tyr143, Gly144, Ser146, Ala145, His121, Ser123, and Glu277 may play a critical role in flavor presentation by molecular docking with T1R1/T1R3. The paper could provide a fast and reliable method for screening umami peptides as well as lay the foundation for novel strategies for evaluating umami taste.
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http://dx.doi.org/10.1016/j.foodchem.2022.133504DOI Listing
June 2022

Treatment Strategy of Metastatic Nasopharyngeal Carcinoma With Bone Marrow Involvement-A Case Report.

Front Oncol 2022 7;12:877451. Epub 2022 Jun 7.

Department of Radiation Oncology, The Second Affiliated Hospital of Zhejiang University, School of Medicine, Hangzhou, China.

Bone marrow involvement (BMI) of solid tumors is a special type of distant metastasis. It has an occult onset, atypical clinical and laboratory features, and a high mortality. We present a nasopharyngeal carcinoma case with cervical and axillary lymph nodes, bilateral lung, multiple bone, and bone marrow metastases, who was treated chemotherapy plus targeted therapy under the guidance of a patient-derived tumor xenograft (PDTX) model, followed by maintenance chemotherapy plus immunotherapy. The patient's symptoms were relieved after four cycles of chemotherapy plus targeted therapy. His bone marrow biopsy turned negative after 7 months of therapy. In addition, his total peripheral T cells as well as the proportion of CD8 T cells increased during the course of therapy. The combination of chemotherapy, targeted therapy, and immunotherapy provides an effective antitumor regimen for advanced NPC patients with BMI.
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http://dx.doi.org/10.3389/fonc.2022.877451DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9209652PMC
June 2022

Antitumor Effect of Pseudolaric Acid B Involving Regulation of Notch1/Akt Signaling Response in Human Hepatoma Cell In Vitro.

Evid Based Complement Alternat Med 2022 14;2022:5353686. Epub 2022 Jun 14.

School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, Gansu, China.

Background: Liver cancer, particularly hepatocellular carcinoma (HCC), is the fourth leading cause of cancer-related death worldwide. Sorafenib is a crucial drug for the treatment of advanced HCC, but it is difficult to meet the challenge of increasing clinical demands due to its severe side effects and drug resistance. Hence, development of novel antitumor drugs is urged. Previous studies showed that pseudolaric acid B (PAB) could reduce the expression of protein kinase B (PKB/Akt), a downstream effector of Notch signaling, facilitating cell apoptosis in HCC. The disruption of Notch signaling was verified to exacerbate malignant progression and drug resistance, however, the antitumor effect of PAB on Notch signaling in HCC remains unclear. Thus, this study aims to investigate the anti-HCC effect of PAB in association with the regulation of Notch1/Akt signaling.

Methods: CCK-8 assay and transwell assay were used to examine the cell proliferation and invasion in Huh7 cells after treatment with PAB and a Notch inhibitor DAPT. Moreover, the cell cycle of Huh7 cells after treatment with PAB was analyzed using flow cytometry. Finally, the changes of Notch1, Jagged1, Hes1, and Akt expression at the protein and mRNA level in Notch1/Akt signaling in Huh7 cells after treatment with PAB and DAPT were analyzed using immunofluorescence assay and real-time qPCR.

Results: The proliferation rate of Huh7 cells exposed to PAB of 0.5, 1, 2, 4, 8, 10, 20, 40, 80, 100, and 200 mol/L revealed a time-and dose-dependent decrease showing cell cycle arrest at G2/M phase ( < 0.05). Furthermore, compared with the untreated group, at the concentration of 40 mol/L, the proliferation rate and invasion rate of Huh7 cells in PAB, DAPT, and PAB-DAPT combination (PAB + DAPT) group were significantly decreased ( < 0.05), but the PAB + DAPT showed no synergistic antiproliferation and anti-invasion effect in comparison with PAB treatment alone ( > 0.05). In addition, compared with the untreated group, PAB and DAPT alone significantly downregulated the expression of Notch1, Jagged1, Hes1, Akt mRNA, or/and protein in Huh7 cells ( < 0.05), but there was no significant difference in synergistic downregulated effect between the PAB + DAPT group and the PAB group ( > 0.05).

Conclusion: PAB can suppress proliferation and invasion of HCC cells through downregulating the expression of Notch1/Akt signaling protein and mRNA, and may be a potential novel antitumor drug candidate for the clinical treatment of HCC in the future.
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http://dx.doi.org/10.1155/2022/5353686DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9213129PMC
June 2022

Thermosensitive Hydrogels and Advances in Their Application in Disease Therapy.

Polymers (Basel) 2022 Jun 12;14(12). Epub 2022 Jun 12.

Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China.

Thermosensitive hydrogels, having unique sol-gel transition properties, have recently received special research attention. These hydrogels exhibit a phase transition near body temperature. This feature is the key to their applications in human medicine. In addition, hydrogels can quickly gel at the application site with simple temperature stimulation and without additional organic solvents, cross-linking agents, or external equipment, and the loaded drugs can be retained locally to improve the local drug concentration and avoid unexpected toxicity or side effects caused by systemic administration. All of these features have led to thermosensitive hydrogels being some of the most promising and practical drug delivery systems. In this paper, we review thermosensitive hydrogel materials with biomedical application potential, including natural and synthetic materials. We describe their structural characteristics and gelation mechanism and briefly summarize the mechanism of drug release from thermosensitive hydrogels. Our focus in this review was to summarize the application of thermosensitive hydrogels in disease treatment, including the postoperative recurrence of tumors, the delivery of vaccines, the prevention of postoperative adhesions, the treatment of nervous system diseases via nasal brain targeting, wound healing, and osteoarthritis treatment.
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http://dx.doi.org/10.3390/polym14122379DOI Listing
June 2022

Risk of illness-related school absenteeism for elementary students with exposure to PM and O.

Sci Total Environ 2022 Jun 20:156824. Epub 2022 Jun 20.

State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, 163 Xianlin Avenue, Qixia, Nanjing, 210023, China.

Air pollution addresses short-term health effects on morbidity, especially for children. Assessing the impacts of air pollution on elementary students is critical for developing preparedness response strategies for this sensitive group. In the 2016-17 academic year, up to 687,748 groups of illness-related absence records and the information on whether the absentee had gone to a hospital or not were collected from 2564 elementary schools across Jiangsu Province China. We explored the associations between air pollution and illness-related records using a time-stratified case-crossover analysis with distributed lag non-linear design. An increase of 10 μg/m in the current-day concentration of PM and O was positively associated with illness-related absenteeism overall. The excess risk of absenteeism was 4.52 % (95%CI 4.37-4.67 %) for PM and 0.25 % (95%CI 0.01-0.36 %) for O. The risk associated with O was boosted for the frequent absentees who tended to have basic diseases or were more vulnerable to infectious diseases. Students in 43.1 % illness-related absenteeism, mainly due to highly infectious diseases, only received home nursing without going to a hospital. The increase in the number of illness cases associated with PM and O estimated based on the illness-related absence data was 41.5 % and 18.6 % higher than that evaluated based on hospital visit records. Such underestimations persisted in sensitivity analyses and persisted in subgroups classified by gender or grade. Together, the performance of illness-related absence records far outweighed that of hospital visit data regarding the thorough evaluation of air pollution-related illness cases for elementary students. Improvement in air quality and home health care education are warranted as well for the health benefits of children.
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http://dx.doi.org/10.1016/j.scitotenv.2022.156824DOI Listing
June 2022

The Role of Changes in Cumulative Lipid Parameter Burden in the Pathogenesis of Type 2 Diabetes Mellitus: A Cohort Study of People Aged 35-65 Years in Rural China.

Diabetes Metab Syndr Obes 2022 16;15:1831-1843. Epub 2022 Jun 16.

Key Laboratory of Cardio Cerebrovascular Diseases, Ministry of Education, Gannan Medical University, Ganzhou, People's Republic of China.

Purpose: The main purpose of this study was to examine the effect of the cumulative exposure of blood lipid parameters on type 2 diabetes mellitus (T2DM). Another purpose was to explore whether the cumulative burden of blood lipid parameters plays a certain role in the pathogenesis of diet affecting T2DM.

Patients And Methods: A total of 63 cases of diabetes occurred from 2017 to 2020, with an incidence density of 3.71 person-years. The dietary intake of the residents was obtained by using a dietary frequency questionnaire (FFQ). Cumulative lipid parameter burden was calculated according to the number of years (2016-2020) multiplied by total cholesterol (TC), high density lipoprotein (HDL), low density lipoprotein (LDL) and triglyceride (TG). A Cox proportional hazard model was used to estimate the effect of cumulative lipid burden on T2DM. A mediating analysis of accelerated failure time (AFT) was used to investigate the mediating effects of certain foods, the cumulative lipid parameter burden and T2DM.

Results: A higher cumulative TG load corresponded to a higher risk of T2DM onset ( =0.021). After adjusting for covariates, the highest quartile cumulative TG burden had a 3.462 times higher risk of T2DM than that in the lowest quartile (=3.462, 95% : 1.297-9.243). Moreover, a higher cumulative HDL load corresponded to a lower risk of T2DM onset ( =0.006). After adjusting for covariates, the risk of T2DM was 0.314-fold lower in the highest quartile of cumulative HDL burden than that in the lowest quartile (=0.314, 95% : 0.131-0.753). Cumulative TG burden partially mediated the association between red meat and T2DM.

Conclusion: The increase in cumulative HDL burden and the decrease in cumulative HDL burden are related to the incidence of T2DM. Cumulative TG burden was shown to play a partial mediating role in the pathogenesis of red meat and diabetes.
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http://dx.doi.org/10.2147/DMSO.S363692DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9208634PMC
June 2022

Efficient Production of 2-O-α-D-Glucosyl Glycerol Catalyzed by an Engineered Sucrose Phosphorylase from Bifidobacterium longum.

Appl Biochem Biotechnol 2022 Jun 22. Epub 2022 Jun 22.

College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 211816, China.

2-O-α-D-Glucosyl glycerol (2-αGG) can be used as a multipurpose anti-aging, cell-stimulating, and skin moisturizing agent in the cosmetic industry. Sucrose phosphorylase (SPase) has been widely used in the production of 2-αGG. In this paper, the gene encoding sucrose phosphorylase from Bifidobacterium longum (BlSP) was inserted into pRSF-Duet-1 to construct the recombinant plasmid pRSF-BlSP and was functionally expressed in E. coli BL21(DE3) to be used as a biocatalyst for the synthesis of 2-αGG firstly. The mutations of BlSP were carried out based on alanine scanning, and a positive mutant G293A with a 50% increase in activity for 2-αGG production was identified. Mutant G293A has less K and bigger k/K towards glycerol than the parental BlSP. Subsequently, the production of 177.6 g/L 2-αGG was attained from 1 M sucrose and 1.2 M glycerol catalyzed by 17 mg/mL G293A mutant. This study indicated that BlSP has good potential in the production of 2-αGG.
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http://dx.doi.org/10.1007/s12010-022-03939-zDOI Listing
June 2022

Retraction Note: Long non-coding RNA TPT1-AS1 promotes cell growth and metastasis in cervical cancer via acting AS a sponge for miR-324-5p.

J Exp Clin Cancer Res 2022 06 22;41(1):207. Epub 2022 Jun 22.

Department of Abdominal Oncology, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510700, China.

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http://dx.doi.org/10.1186/s13046-022-02418-xDOI Listing
June 2022

MSTN modification in goat mediated by TALENs and performance analysis.

Yi Chuan 2022 Jun;44(6):531-542

Department of Basic Medicine, School of Health and Nursing, Wuxi Taihu University, Wuxi 214000, China.

Myostatin (MSTN) is a negative regulator of skeletal muscle growth and development. It can inhibit the proliferation of myoblasts and serve as an important candidate gene for animal breed improvement. Mutations of the MSTN gene can cause extensive skeletal muscle hyperplasia and hypertrophy, resulting in "double muscle" symptoms. This leads to reduction of animal fat differentiation and increase of muscle content, thereby meeting the demand for quality consumption of animal meat in the market. In order to obtain a double-muscle phenotype using mutant MSTN gene in cloned goat, the goat MSTN gene was target-modified by TALENs. In this study, the TALENs expression vector was designed and constructed in the first exon sequence of the goat MSTN gene, which was then transfected into the goat fetal fibroblasts. The resistant cell lines were obtained by puromycin selection, and the cell lines with the MSTN gene mutations were analyzed by PCR and gene sequencing, thereby identifying the mutation type(s). The MSTN gene mutant cell lines were used as the nuclear donor cells in somatic cell nuclear transfer procedures in goats, and The morphological structure of the muscle tissue of the goats with MSTN gene mutations was analyzed by tissue section. The body weight of the cloned goats were monitored at different months of age, which provided the growth trend of their weight at different developmental stages. The results show that a total of 109 MSTN gene mutant cell lines were obtained. The mutation efficiency was 79.0% (109/138), of which 46 were biallelic mutations, accounting for 33.3% (46/138) of the total cell lines. Four MSTN gene mutant cell lines (1 biallelic homozygous mutation, 3 non-homozygous mutations) with good growth status were selected for somatic cell nuclear transfer in 12 recipients, of which 4 were pregnant by B-ultrasound at 30 days, indicating the a 33.3% (4/12) pregnancy rate. Two cloned goats were born at the end of the pregnancy. Sequencing analysis showed that there was no mutation in one allele of the M-1 cloned lamb, and the other allele harbored a 3 bp-deletion. The M-2 cloned lamb harbored a 1 bp base insertion in one allele of the MSTN gene, and a deletion of 13 bp in the other allele, resulting in mutations in both alleles and the loss of the protein-coding sequence of MSTN after the mutation site. In addition, the muscle fibers of cloned M-1 goats are tightly arranged and thick, and their monthly body weight is higher than that of normal wild-type goats. However, it is still consistent with the growth trend of normal wild-type goats and the M-1 goats can develop into healthy adults. In summary, this study showed that goat fetal fibroblasts with the multiple MSTN gene mutations were successfully obtained by TALENs technology, and cloned goats with mutant MSTN genes could be generated by somatic cell nuclear transfer method, thereby providing a technical foundation for the cultivation of the "double muscle" phenotype goats, and serving as a reference method for the preparation of other transgenic animals in the future.
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http://dx.doi.org/10.16288/j.yczz.22-011DOI Listing
June 2022

Co-assembly of egg white-derived peptides and protein-polysaccharide complexes for curcumin encapsulation: The enhancement of stability, redispersibility, and bioactivity.

Food Chem 2022 Jun 16;394:133496. Epub 2022 Jun 16.

Jilin Provincial Key Laboratory of Nutrition and Functional Food, and College of Food Science and Engineering, Jilin University, Changchun 130062, People's Republic of China. Electronic address:

In this study, a nanocomposite was developed by introducing egg white-derived peptides (EWDP) into protein-polysaccharide complexes to trigger the self-assembly of EWDP for encapsulating curcumin (Cur) via the pH-driven method. In this system, EWDP could cooperate with protein-polysaccharide complexes to exert superior colloidal properties with excellent Cur aqueous solubility, redispersibility, and physical stability and act as a bioactivity amplifier to endow the delivery system with the synergistic antioxidant activity. This phenomenon was ascribed to the additional hydrophobic cavities, hydrogen bonding, and electrostatic interactions organized by EWDP. Additionally, the presence of EWDP could considerably boost the cellular antioxidant activity of Cur by decreasing reactive oxygen species (ROS) levels, improving free radical scavenging capacity, and recovering the activity of endogenous antioxidant enzymes. These findings might open up an avenue to reinforce lipophilic nutraceuticals' physicochemical properties and functionalities based on the co-assembly of food-derived peptides and protein-polysaccharide complexes.
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http://dx.doi.org/10.1016/j.foodchem.2022.133496DOI Listing
June 2022

A Non-canonical MITE in WOX11 Promoter is Associated With Robust Crown Root in Rice.

Plant Cell Physiol 2022 Jun 21. Epub 2022 Jun 21.

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China.

The formation of tissues and organs in multicellular organisms are tightly controlled by transcriptional programs determined by temporal and spatial patterns of gene expression. As an important regulator of rice crown root development, WOX11 is essential for crown root formation and its transcript level is positively correlated with crown roots biomass. However, how WOX11 is regulated during crown root primordium emergence and outgrowth still remains unknown. In this study, variations of WOX11 genomic sequence were analyzed, and the highest genetic diversity was found within its promoter, which contained a non-canonical miniature inverted-repeat transposable element (ncMITE) sequence. Analysis of WOX11 promoter driven reporter gene GUS (β-glucuronidase) transgenic plants pWOX11(ncMITE+):GUS and pWOX11(ncMITE-):GUS uncovered higher GUS expression level in crown roots of pWOX11(ncMITE+):GUS plants. Furthermore, pWOX11(ncMITE+):WOX11-FLAG in wox11 background could complement crown root number and length compared to those of wild type (WT), while pWOX11(ncMITE-):WOX11-FLAG could not. These results suggested that the ncMITE was positively associated WOX11 transcripts in rice crown roots. In addition, DNA methylation nearby ncMITE region attenuated the activation effect of the ncMITE on WOX11 expression, which might be also the cause conferred to the root-specific expression of WOX11. This work provides novel insight into WOX11 expression regulation and reveals a promising target for genetic improvement of root architecture in rice.
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http://dx.doi.org/10.1093/pcp/pcac075DOI Listing
June 2022

The effect of high-risk HPV E6/E7 mRNA on the efficacy of topical photodynamic therapy with 5-aminolevulinic acid for cervical high-grade squamous intraepithelial lesions.

Photodiagnosis Photodyn Ther 2022 Jun 17:102974. Epub 2022 Jun 17.

Department of Gynecology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China. Electronic address:

Background: E6 and E7 high-risk human papillomavirus (HR-HPV) oncoproteins are closely associated with the initiation and progression of cervical cancer (CC) and pre-cancerous lesions. Cervical high-grade squamous intraepithelial lesions (HSIL), as pre-cancerous lesions, have a 5% chance of progressing to invasive cancer. Topical 5-aminolevulinic acid photodynamic therapy (ALA-PDT) is a novel non-invasive targeted therapy for intraepithelial lesions. Herein, we analyzed the effect of HR-HPV E6/E7 mRNA on ALA-PDT for cervical HSIL.

Methods: A retrospective analysis of 148 HR-HPV-positive patients diagnosed with cervical HSIL and receiving ALA-PDT was carried out. ALA-PDT was performed with 20% ALA thermosensitive gel, and irradiation at wavelength of 635 nm and density of 80-100 J/cm for 20-30 min. The therapeutic instruments of LED and semiconductor laser were applied for cervical lesions and lesions in endocervical canal, respectively. All patients were tested for HPV E6/E7 mRNA before and after PDT, and then followed up at 3, 6, and 12 months after treatment, and every six months thereafter.

Results: At the 6-month follow up, the complete remission (CR) rate of patients' lesions was 86.5% (128/148), whereas the total HPV clearance rate was 72.3% (107/148). It was evident that positive E6/E7 mRNA before treatment had a significant effect on HPV clearance rate (66.3% VS 81.4%, P=0.045) and CR rate (80.9% VS 94.9%, P=0.015). The E6/E7 mRNA associated with HPV16/18 and HPV16/18 combined with other HR-HPV (HPV16/18 and other HR-HPV) affected HPV clearance (P=0.035) and lesions CR (P=0.039), respectively. Moreover, persistently positive E6/E7 mRNA after treatment was closely associated with poor efficacy (HPV clearance rate: P=0.000, CR rate: P=0.000). Throughout the follow up period, two cases recurred but none of the patients progressed.

Conclusions: This study has shown that ALA-PDT is an effective, safe, and alternative treatment for cervical HSIL, especially for the patients of childbearing age. However, its efficacy is relatively poor in patients with persistently positive E6/E7 mRNA before and after treatment, who are insensitive to ALA-PDT.
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http://dx.doi.org/10.1016/j.pdpdt.2022.102974DOI Listing
June 2022

Botany, traditional uses, phytochemistry, pharmacological and toxicological effects of Croton tiglium Linn.: a comprehensive review.

J Pharm Pharmacol 2022 Jun 20. Epub 2022 Jun 20.

School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, P.R. China.

Objectives: Croton tiglium Linn. (Euphorbiaceae) is an ancient medicinal plant that has been used for a long time, which is widely distributed in tropical and subtropical regions. And it is widely used for defecation, induced labour, treatment of gastrointestinal diseases, headache, as well as rheumatoid arthritis.

Key Findings: Approximately 150 compounds have been isolated and identified from the seeds, stems, leaves and branches of C. tiglium, including fatty acids, terpenoids, alkaloids, the plants proteins and other types of components. Based on a wide range of biological properties, C. tiglium has a wide range of pharmacological effects, such as antitumor, anti-HIV, analgesic, anti-inflammatory and antibacterial effects.

Summary: The review aims to provide a critical and comprehensive evaluation of the botany, phytochemistry, pharmacology and toxicity of C. tiglium, with a vision for promoting further pharmaceutical research to explore its complete potential for better clinical application. The tigliane diterpenoids have been the most studied compounds isolated from C. tiglium, which showing a variety of biological activities, but there is insufficient evidence to explain the mechanism of action. In addition, C. tiglium may have potential toxic effects, and it is necessary to reduce the toxic effects to ensure the safety of clinical medication, which may promote the discovery and development of new drugs.
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http://dx.doi.org/10.1093/jpp/rgac040DOI Listing
June 2022

Effects of Biomimetic Micropatterned Surfaces on the Adhesion and Morphology of Cervical Cancer Cells.

ACS Omega 2022 Jun 27;7(23):19913-19919. Epub 2022 May 27.

State Key Laboratory of Solid Lubrication, , Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China.

It has been demonstrated that micropatterned surfaces have an important influence on modulating cellular behavior. In recent years, with the rapid development of microfabrication techniques and in-depth study of nature, an increasing number of patterned structures imitating natural organisms have been successfully fabricated and widely evaluated. However, there are only a few reports about biomimetic patterned microstructures in biologically related fields. In our work, micropatterned polydimethylsiloxane (PDMS) was fabricated by mimicking the surface microstructures of natural Trifolium and leaves using the template duplication method. The interactions between the two types of biomimetic micro-PDMS surfaces and two kinds of human cervical cancer cells (HeLa and SiHa) were investigated. HeLa and SiHa cells cultured on the two micropatterned PDMS samples exhibited more stretchable morphology, higher diffusion, and a much lower nuclear/cytoplasmic ratio than those cultured on flat PDMS surfaces, indicating a higher adhesion area of the cells. Both of the micro-PDMS substrates were found to induce significantly different morphological changes between HeLa and SiHa cells. This suggests that the micropatterned structure affects cell adhesion and morphology correlated with their surface geometric structure and roughness. The results reveal that biomimetic micropatterned surfaces from natural leaves significantly regulate the morphology and adhesion behavior of cervical cancer cells and are believed to be the new platforms for investigating the interaction between cells and substrates.
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http://dx.doi.org/10.1021/acsomega.2c01703DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9202008PMC
June 2022

Urine ALCAM, PF4 and VCAM-1 Surpass Conventional Metrics in Identifying Nephritis Disease Activity in Childhood-Onset Systemic Lupus Erythematosus.

Front Immunol 2022 26;13:885307. Epub 2022 May 26.

Department of Biomedical Engineering, University of Houston, Houston TX, United States.

Objectives: Serial kidney biopsy for repeat evaluation and monitoring of lupus nephritis (LN) in childhood-onset Systemic Lupus Erythematosus (cSLE) remains challenging, thus non-invasive biomarkers are needed. Here, we evaluate the performance of ten urine protein markers of diverse nature including cytokines, chemokines, and adhesion molecules in distinguishing disease activity in cSLE.

Methods: Eighty-four pediatric patients meeting ≥4 ACR criteria for SLE were prospectively enrolled for urine assay of 10 protein markers normalized to urine creatinine, namely ALCAM, cystatin-C, hemopexin, KIM-1, MCP-1, NGAL, PF-4, Timp-1, TWEAK, and VCAM-1 by ELISA. Samples from active renal (LN) and active non-renal SLE patients were obtained prior to onset/escalation of immunosuppression. SLE disease activity was evaluated using SLEDAI-2000. 59 patients had clinically-active SLE (SLEDAI score ≥4 or having a flare), of whom 29 patients (34.5%) were classified as active renal, and 30 patients (35.7%) were active non-renal. Twenty-five healthy subjects were recruited as controls.

Results: Urine concentrations of ALCAM, KIM-1, PF4 and VCAM-1 were significantly increased in active LN patients versus active non-renal SLE, inactive SLE and healthy controls. Five urine proteins differed significantly between 2 (hemopexin, NGAL, MCP1) or 3 (Cystatin-C, TWEAK) groups only, with the highest levels detected in active LN patients. Urine ALCAM, VCAM-1, PF4 and hemopexin correlated best with total SLEDAI as well as renal-SLEDAI scores (p < 0.05). Urine ALCAM, VCAM-1 and hemopexin outperformed conventional laboratory measures (anti-dsDNA, complement C3 and C4) in identifying concurrent SLE disease activity among patients (AUCs 0.75, 0.81, 0.81 respectively), while urine ALCAM, VCAM-1 and PF4 were the best discriminators of renal disease activity in cSLE (AUCs 0.83, 0.88, 0.78 respectively), surpassing conventional biomarkers, including proteinuria. Unsupervised Bayesian network analysis based on conditional probabilities re-affirmed urine ALCAM as being most predictive of active LN in cSLE patients.

Conclusion: Urinary ALCAM, PF4, and VCAM-1 are potential biomarkers for predicting kidney disease activity in cSLE and hold potential as surrogate markers of nephritis flares in these patients.
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http://dx.doi.org/10.3389/fimmu.2022.885307DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204340PMC
June 2022

Characteristics of Body Composition Estimated by Air-Displacement Plethysmography in Chinese Preschool Children.

Front Public Health 2022 3;10:926819. Epub 2022 Jun 3.

Department of Epidemiology, Capital Institute of Pediatrics, Beijing, China.

Objective: To describe the characteristics of body composition by air-displacement plethysmography (ADP) among Chinese preschool children.

Methods: Preschool children were recruited from three kindergartens. Adiposity indices were evaluated using the ADP method. BMI, fat mass index (FMI), fat-free mass index (FFMI) and waist-to-height ratio (WHtR) were calculated. Overweight and obesity were diagnosed using the WHO reference. Analyses were executed by SPSS and MedCalc software. Smoothed curves were constructed using the lambda-mu-sigma (LMS) method.

Results: This study evaluated the growth trend for body composition of ADP-based body fat indices based on a relatively large sample of preschool children, the first ever reported in China. A total of 1,011 children aged 3-5 years comprised our study population. BMI and FFMI increased with age, but the slope ( = 0.710) and y intercept ( = 0.132) in the BMI trend analysis demonstrated no differences between boys and girls. For the FFMI trend lines, the slope was significantly higher for boys than for girls ( = 0.013). The percentage of fat mass (FM%), FMI, and WHtR were negatively correlated with age for both sexes, except for FMI in girls ( = 0.094). The 95% CI regression lines for FM% according to different weight statuses intersected.

Conclusions: ADP is applicable to estimating body composition among Chinese preschool children. Misclassifications might occur when overweight/obese status is defined based on surrogate indices.
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http://dx.doi.org/10.3389/fpubh.2022.926819DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204163PMC
June 2022

[Mechanism and material basis of Sparganii Rhizoma and vinegar-processed Sparganii Rhizoma in treatment of hyperlipidemia based on network pharmacology].

Zhongguo Zhong Yao Za Zhi 2022 May;47(10):2767-2777

School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210046, China.

The present study explored the effective components, functional targets, and mechanism of Sparganii Rhizoma(vinegar-processed Sparganii Rhizoma) in the treatment of hyperlipidemia based on network pharmacology and experimental verification. In the network pharmacology, the screening of active components, target prediction, and pathway enrichment analysis of Sparganii Rhizoma were carried out, followed by the comparison with targets and pathways related to hyperlipidemia. In the experimental verification, the hyperlipidemia model in rats was induced to detect hemorheological parameters and coagulation function. The liver index was observed by HE staining, and PCR technology was used to verify the results of the network pharmacological analysis. Compared with the model group, the Sparganii Rhizoma and vinegar-processed Sparganii Rhizoma groups showed decreased liver index(P<0.05), reduced liver lipid deposition, dwindled serum low-density lipoprotein cholesterol(LDL-c) level(P<0.05), diminished blood viscosity, and increased prothrombin time(PT), thrombin time(TT), and activated partial thrombin time(APTT)(P<0.05). As revealed by the PCR assay, Sparganii Rhizoma could affect LDL-c and high-density lipoprotein cholesterol(HDL-c) levels and reduce the inhibitory effect of cholesterol ester transporter by regulating the expression of Apol2, Apof, and Stab2, thereby treating hyperlipidemia. Vinegar-processed Sparganii Rhizoma could enhance triglyceride metabolism and cholesterol reversal by regulating the expression of Hmgcr, Hmgcs2, Abca1, Abcg1, Cyp7 b1, and Stab2. Compared with the Sparganii Rhizoma, the vinegar-processed one was potent in treating hyperlipidemia. The active components of Sparganii Rhizoma in the treatment of hyperlipidemia may be L-alpha-palmitin,(1S,2S)-1,2-bis(2-furyl)ethane-1,2-diol, cis-zimtsaeure, o-acetyl-p-cresol, sanleng, and 9-hexadecenoic acid. Based on the network pharmacology and experimental verification, this study preliminarily explored the potential active components and possible mechanism of Sparganii Rhizoma in the treatment of hyperlipidemia, which is expected to provide a certain basis for in-depth research on active components, mechanism, and clinical application.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20211227.401DOI Listing
May 2022

Comparative study of topical 5-aminolevulinic acid photodynamic therapy (5-ALA-PDT) and surgery for the treatment of high-grade vaginal intraepithelial neoplasia.

Photodiagnosis Photodyn Ther 2022 Jun 16:102958. Epub 2022 Jun 16.

Department of Gynecology, Affiliation hospital of The First Affiliated Hospital of Zhengzhou University 450003, China. Electronic address:

Objective: To compare the clinical efficacy and safety of 5-aminolevulinic acid photodynamic therapy (ALA-PDT) and local surgical excision in the treatment of high-grade vaginal intraepithelial neoplasia (VAIN).

Methods: This was a retrospective study of a single center. Patients with biopsy-proven high-grade VAIN were counseled on the option of excision or ALA-PDT. After treatment patients were followed up at 3, 6 and 12 months and every 6 months afterwards. Clinical data of two groups were analyzed.

Results: Sixty patients were treated with ALA-PDT and 40 patients underwent excision surgery. The clinical characteristics of patients in the two groups were similar. The mean ages of patients treated with ALA-PDT and local surgical excision were 41.93+13.08 (18-70) and 43.25+11.64 (22-61) years old, respectively. The treatment times of PDT for patients in the ALA-PDT group were 5.48±1.41 (2-8) times. At 3-6 months after treatment, the overall complete remission (CR) rate was 93.33% (56/60) in PDT group and 82.5% (33/40) in local surgical excision group. In PDT group, a 12- and 24-month follow-up, the total HPV clearance rate was 68.97% and 77.55%, respectively. At 12-month and 2-year follow-up after local surgical excision, the total HPV clearance rate was 60% and 64.52%, respectively. Further analysis showed that the clinical efficacy was comparable between the two treatments. Patients over 45 years old exhibited a lower HPV clearance rate (P<0.05) than those under 45 years old. It was also observed that persistent HPV infection is a major risk factor for persistent disease. Further comparative analyses showed no significant difference in the CR rate and treatment times between VAINII and VAINIII in ALA-PDT group. Moreover, 4 patients (11.36%) developed recurrent lesions during follow-up after ALA-PDT, whereas 3 patients (8.33%) in the local excision group developed recurrent lesions. In addition, there was no significant difference in the recurrent rate between two groups.

Conclusions: ALA-PDT shows similar efficacy and less side effects compared to local surgical excision.
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http://dx.doi.org/10.1016/j.pdpdt.2022.102958DOI Listing
June 2022

Cell membrane-camouflaged inorganic nanoparticles for cancer therapy.

J Nanobiotechnology 2022 Jun 18;20(1):289. Epub 2022 Jun 18.

Institute for Translational Medicine, Department of Stomatology, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, 266003, China.

Inorganic nanoparticles (INPs) have been paid great attention in the field of oncology in recent past years since they have enormous potential in drug delivery, gene delivery, photodynamic therapy (PDT), photothermal therapy (PTT), bio-imaging, driven motion, etc. To overcome the innate limitations of the conventional INPs, such as fast elimination by the immune system, low accumulation in tumor sites, and severe toxicity to the organism, great efforts have recently been made to modify naked INPs, facilitating their clinical application. Taking inspiration from nature, considerable researchers have exploited cell membrane-camouflaged INPs (CMCINPs) by coating various cell membranes onto INPs. CMCINPs naturally inherit the surface adhesive molecules, receptors, and functional proteins from the original cell membrane, making them versatile as the natural cells. In order to give a timely and representative review on this rapidly developing research subject, we highlighted recent advances in CMCINPs with superior unique merits of various INPs and natural cell membranes for cancer therapy applications. The opportunity and obstacles of CMCINPs for clinical translation were also discussed. The review is expected to assist researchers in better eliciting the effect of CMCINPs for the management of tumors and may catalyze breakthroughs in this area.
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http://dx.doi.org/10.1186/s12951-022-01475-wDOI Listing
June 2022

Pediatric patients in the new wave of SARS-CoV-2 infection in Shanghai, China.

World J Pediatr 2022 Jun 17. Epub 2022 Jun 17.

Molecular Medical Center, Shanghai Key Laboratory of Birth Defects, Department of Neonatology, Children's Hospital of Fudan University, 399 Wanyuan Road, Shanghai, 201101, China.

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http://dx.doi.org/10.1007/s12519-022-00570-0DOI Listing
June 2022

Myoneurin regulates BMP signaling by competing with Ppm1a for Smad binding.

iScience 2022 Jun 30;25(6):104495. Epub 2022 May 30.

Division of Cell, Developmental and Integrative Biology, School of Medicine, South China University of Technology, Guangzhou 510006, China.

A delicate balance of BMP activity is critical for tissue formation and organogenesis. However, the mechanical molecular details in ensuring the proper duration and intensity of BMP signaling have yet to be fully elucidated. Here, we identified a zebrafish mutant with a disrupted gene encoding for the BTB/POZ and zinc finger protein myoneurin (Mynn). mutants exhibited severe loss of pharyngeal cartilage elements, owing to poor proliferation, blocked differentiation, and low viability of cranial neural crest cells. Depletion of in both zebrafish embryos and mammalian cells led to a reduction of the BMP signal activity. Mechanistically, Mynn interacts with Smad proteins in the nucleus, thereby disrupting the association between Smad protein and the phosphatase Ppm1a. Ultimately, this interaction prevents Smad dephosphorylation. More broadly, our findings may provide a new strategy to balance BMP signal activity via competitive binding of Mynn and Ppm1a to Smad proteins during pharyngeal cartilage formation.
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http://dx.doi.org/10.1016/j.isci.2022.104495DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9194458PMC
June 2022

Association of metabolic syndrome with TyG index and TyG-related parameters in an urban Chinese population: a 15-year prospective study.

Diabetol Metab Syndr 2022 Jun 15;14(1):84. Epub 2022 Jun 15.

Department of Cardiology, West China Hospital, Sichuan University, No. 37 Guo Xue Alley, Chengdu, 610041, China.

Background: The metabolic syndrome (Mets) is a multiplex risk factor for atherosclerotic cardiovascular diseases. The aims of the study were to assess the association of the Mets with TyG index and TyG-related parameters in an urban Chinese population.

Methods: The data were collected in 1992 and then again in 2007 from the same group of 590 individuals (363 males and 227 females) without Mets in 1992. The fasting lipid profile and blood glucose were measured. TyG index and related parameters were calculated, and Mets defined according to the harmonized criteria. The area under the curve (AUC) of receiver operating characteristic curves was used to evaluate TyG index and related parameters for their diagnostic ability to identify people with Mets. Odd ratios (OR) for Mets prediction were calculated using stepwise logistic regression analyses.

Results: The incidence of Mets was 18.64% over the 15-year follow-up period.During 15 years' follow-up, TyG-waist to height ratio (TyG-WHtR) shows the largest AUC for Mets detection (0.686) followed by TyG-waist circumference (TyG-WC) (0.660), TyG-waist-to-hip ratio (TyG-WHpR) (0.564), and TyG index (0.556) in all participants. Gender analysis revealed that TyG-WHtR and TyG-WC have the largest AUC in both genders. TyG-WHtR significantly predicted Mets in all participants, with an unadjusted odds ratio of 5.63 (95% CI 3.23-9.83 P < 0.001). Associations remained significant after adjustment for smoking, drinking, physical exercise and components of Mets.

Conclusions: TyG-WHtR might be a strong and independent predictor for Mets in all participants in an urban Chinese population. TyG-related markers that combine obesity markers with TyG index are superior to other parameters in identifying Mets in both genders.
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http://dx.doi.org/10.1186/s13098-022-00855-4DOI Listing
June 2022

Public anxiety through various stages of COVID-19 coping: Evidence from China.

PLoS One 2022 16;17(6):e0270229. Epub 2022 Jun 16.

School of the Environment, Nanjing University, Xianlin Campus, Nanjing, China.

As countries underwent the initiation, peak, post-peak, and early vaccination stages of COVID-19, the changing risk perception, coping behaviors and corresponding psychological stress experienced by the public over time was rarely reported. We conducted a national scale panel study using social-psychological data collected from 5,983 questionnaires to investigate the interactions between anxiety level, risk perception and coping behavior during different stages of COVID-19 in China. We found that sustained perceiving worries of being infected, first due to domestic and then global pandemic, contributed to the persistent high proportion of respondents with anxiety disorders which even gradually increased over time (56.1% during initiation to 60.4% during early vaccination). Gender was the strongest predictor of anxiety at all stages, with females having less confidence in COVID-19 control and always suffering from much higher anxiety levels than males even during the post peak stage. Excessive protective behavior and frequency of access to COVID-related news also contributed to public anxiety. Additionally, public risk perception was significantly associated with their willingness to vaccinate. The findings verify the feasibility of taking stage-specific and gender-based risk communication strategies to alleviate the pandemic-related public anxiety and promote vaccination by influencing public risk perception and guiding coping behaviors.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0270229PLOS
June 2022

A novel LC-MS/MS method for the simultaneous analysis of selected fat-soluble vitamins in serum obtained from pediatric patients with pneumonia.

Anal Methods 2022 Jun 15. Epub 2022 Jun 15.

Clinical Central Laboratory, Capital Institute of Pediatrics, Beijing 100020, China.

Several observational studies have reported associations between low levels of fat-soluble vitamins (A, D and E) and the incidence of pneumonia. Whether infection affects or negatively regulates serum vitamin levels remains controversial. Our aims were to develop and validate a simple-pretreatment and fast method to determine the serum levels of selected fat-soluble vitamins, namely vitamin A (retinol), vitamin D (25-OH-D, 25-OH-D, and 3-epi-25-OH-D), and vitamin E (α-tocopherol), in children suffering from pneumonia during the acute phase and after inflammatory marker recovery. The sample preparation procedure involving protein precipitation and filtration was finished in one step, and separation took 8 min per sample. The calibrations were linear, with > 0.99. Both the intra-run ( = 6) and inter-run ( = 3) precision (relative standard deviation, RSD%) values were below 14.61%. The spiked recoveries at 3 concentrations ranged from 80.97 to 111.91%. Accuracies were calibrated using both National Institute of Standards and Technology serum (NIST 968f) and external quality assurance (EQA) samples offered by the National Center of Clinical Laboratories of China, and the relative error (RE%) values ranged from -13.17% to 12.53%. Clinical sample analysis revealed that infection did alter the serum retinol concentration and it did not alter the 25-OH-D and α-tocopherol levels in young children with pneumonia.
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http://dx.doi.org/10.1039/d2ay00491gDOI Listing
June 2022

Electrochemical reactors for continuously decentralized H2O2 production.

Angew Chem Int Ed Engl 2022 Jun 14. Epub 2022 Jun 14.

Shanghai Jiao Tong University, Environmental Science and Engineering, 800 Dongchuan Road, 44106, Shanghai, CHINA.

The global utilization of H 2 O 2 is currently around 4 million tons per annum and expected to continue growing in the future. H 2 O 2 is mainly produced by the anthraquinone process, which involves multiple steps in terms of alkylanthraquinone hydrogenation/oxidation in organic solvents and liquid-liquid extraction of H 2 O 2 . The energy-intensive and environmentally unfriendly anthraquinone process doesn't meet the need of the sustainable and low-carbon development. The electrocatalytic two-electron (2e - ) oxygen reduction reaction (ORR) driven by the renewable energy (e.g. solar and wind power) offers a more economical, low-carbon and greener route to produce H 2 O 2 . However, continuous and decentralized H 2 O 2 electrosynthesis still remains many challenges. This review firstly summarizes the development of devices for H 2 O 2 electrosynthesis, and then introduces each component, assembly process and some optimization strategies.
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http://dx.doi.org/10.1002/anie.202205972DOI Listing
June 2022

Silver nanoparticles induced hippocampal neuronal damage involved in mitophagy, mitochondrial biogenesis and synaptic degeneration.

Food Chem Toxicol 2022 Jun 10;166:113227. Epub 2022 Jun 10.

Key Laboratory of Environmental Medicine and Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, 210009, China. Electronic address:

Silver nanoparticles (AgNPs) could accumulate in the central nervous system (CNS) and induce neurotoxicity for their widespread use in industry and medicine. Mitochondria are vulnerable to toxicity of AgNPs, however, their role in the neurotoxicity remains unclear. This study aimed to evaluate AgNPs-induced synaptic degeneration in mouse hippocampal neurons (at a dose of 12-120 mg/kg BW via intravenous injection), and to further investigate mechanism of mitophagy, mitochondrial biogenesis process in the neurotoxicity. The results indicated that AgNPs accumulated in mouse hippocampal neurons and induced neurological deficits of learning and memory, which involved in synaptic degeneration accompanied with mitochondrial damage. Mechanistically, AgNPs exposure increased protein expression of PTEN-induced kinase 1 (PINK1), Parkin and inhibited peroxisome proliferator-activated receptor coactivator 1 alpha (PGC-1α) protein expression, caused disturbed mitophagy and mitochondrial biogenesis. AgNPs also induced synaptic damage by increasing the protein expression of synaptophysin and decreasing PSD95, MAP2 protein expression. AgNPs exposure even promoted protein expression of amyloid precursor protein (APP) using in amyloid-β (Aβ) cleavage. Furthermore, AgNPs induced hippocampal neuronal synaptic degeneration, mitophagy and mitochondrial biogenesis is dependent on particle-specific AgNPs rather than released silver ions. Our research could provide insights into the regulatory mechanisms of AgNPs-induced neurotoxicity. This study will shed the light of neurotoxicological evaluation of nanoparticles and possible early warning of biomedical applications.
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http://dx.doi.org/10.1016/j.fct.2022.113227DOI Listing
June 2022

Adverse reproductive and developmental consequences of quantum dots.

Environ Res 2022 Jun 10:113666. Epub 2022 Jun 10.

Key Laboratory of Environmental Medicine and Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, People's Republic of China. Electronic address:

Quantum dots (QDs), with a size of 1-10 nm, are luminescent semiconductor nanocrystals characterized by a shell-core structure. Notably, QDs have potential application in bioimaging owing to their higher fluorescence performance than conventional fluorescent dyes. To date, QDs has been widely used in photovoltaic devices, supercapacitors, electrocatalysis, photocatalysis. In recent years, scientists have focused on whether the use of QDs can interfere with the reproductive and developmental processes of organisms, resulting in serious population and community problems. In this study, we first analyze the possible reproductive and development toxicity of QDs. Next, we summarize the possible mechanisms underlying QDs' interference with reproduction and development, including oxidative stress, altered gametogenesis and fetal development gene expression, autophagy and apoptosis, and release of metal ions. Thereafter, we highlight some potential aspects that can be used to eliminate or reduce QDs toxicity. Based on QDs' unique physical and chemical properties, a comprehensive range of toxicity test data is urgently needed to build structure-activity relationship to quickly evaluate the ecological safety of each kind of QDs.
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http://dx.doi.org/10.1016/j.envres.2022.113666DOI Listing
June 2022

Microplastic pollution at Qilianyu, the largest green sea turtle nesting grounds in the northern South China Sea.

PeerJ 2022 7;10:e13536. Epub 2022 Jun 7.

Ministry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences, Hainan Normal University, Haikou, China.

Microplastics, new persistent pollutants, have recently attracted considerable attention. When present in beach sediments, microplastics may adversely affect the nesting and hatching of sea turtles on beaches. In this study, we investigate microplastic pollution at Qilianyu (northeastern Xisha Islands), the largest known nesting ground for green sea turtles () in China. We found that the average abundance of microplastics in the beach surface sediments was 338.44 ± 315.69 thousand pieces·m or 1,353.78 ± 853.68 pieces·m, with foam and fragments as the main microplastic type identified. The microplastic particles were categorised as small and were predominantly within the 0.05-1 mm size category. Most microplastic particles were white (71.31%). Polystyrene and polyethylene were found to be the most common forms of plastic present. Microplastic pollution was not only observed on the beach surface but also at the bottom of nests approximately 60 cm may be harmful to the incubation of sea turtle eggs. We suggest removing plastic litter, especially small pieces of plastic, on beaches to reduce the threat of microplastic pollution to marine life, including sea turtles. Furthermore, the foam used in aquaculture should be recovered and replaced before it becomes fragmented due to age. In addition, regional cooperation between stakeholders in the South China Sea should be strengthened to collectively promote the reduction and cleanup of marine litter.
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http://dx.doi.org/10.7717/peerj.13536DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9186329PMC
June 2022

Effective interventions on health effects of Chinese rural elderly under heat exposure.

Front Environ Sci Eng 2022 25;16(5):66. Epub 2022 May 25.

State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210023 China.

Due to climate change, the heatwave has become a more serious public health threat with aging as an aggravating factor in recent years. There is a pressing need to detect the most effective prevention and response measures. However, the specific health effects of interventions have not been characterized on an individual scale. In this study, an intervention experiment was designed to explore the health effects of heat exposure at the individual level and assess the effects of different interventions based on a comprehensive health sensitivity index (CHSI) in Xinyi, China. Forty-one subjects were recruited randomly, and divided into one control group and three intervention groups. Interventions included education (Educate by lecturing, offering relative materials, and communication), subsidy support (offer subsidy to offset the cost of running air conditioning), and cooling-spray (install a piece of cooling-spray equipment in the yard). Results showed that systolic blood pressure (SBP) and deep sleep duration (DSD) were significantly affected by short-term heat exposure, and the effects could be alleviated by three types of interventions. The estimated CHSI indicated that the effective days of the education group were longer than other groups, while the lower CHSI of the subsidy group showed lower sensitivity than the control group. These findings provide feasible implementation strategies to optimize Heat-health action plans and evaluate the intervention performance.

Electronic Supplementary Material: Supplementary material is available in the online version of this article at 10.1007/s11783-022-1545-4 and is accessible for authorized users.
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http://dx.doi.org/10.1007/s11783-022-1545-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9170494PMC
May 2022

Identification of Immune-Related Genes in Patients with Acute Myocardial Infarction Using Machine Learning Methods.

J Inflamm Res 2022 3;15:3305-3321. Epub 2022 Jun 3.

Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, 210029, People's Republic of China.

Objective: This study aimed to analyze immune-related genes and immune cell components in the peripheral blood of patients with acute myocardial infarction (AMI).

Methods: Six datasets were obtained from the GEO repository comprising 88 healthy samples and 215 AMI samples. We performed the weighted gene co-expression analysis (WGCNA) and five machine learning (ML) methods to identify immune-related genes and construct diagnostic models. CIBERSORT algorithm was adopted for the assessment of the degree of immune infiltration. Finally, RT-PCR, immunofluorescence double and immunohistochemistry were conducted to analyze the expression level of the identification of featured immune-related genes and localization relationship in heart tissue of AMI mouse model.

Results: A total of 496 immune-related DEGs were obtained between AMI and normal samples. WGCNA finally determined the co-expression modules that showed the most significantly positively associated with AMI (r=0.41; P<0.001). Among the five ML models, XGBoost had the highest AUC (0.849) and accuracy (0.812) to discriminate patients with AMI from normal in the validation sets. Furthermore, we found that the proportion of chemokine receptor (CCR), macrophages, neutrophils, and Treg cells in the AMI groups was significantly higher than that in the normal groups. In vitro RT-PCR verification revealed that , and expression increased significantly in the AMI mouse model. Among the 22 immune cells, , and displayed the strongest positive correlation with neutrophils. In MI-mice, stained strongly along the lateral cardiomyocytes, whereas it was weaker in sham mice. Combined immunofluorescence was observed in same parts of the cytoplasm of cardiomyocytes in myocardial infarction area, indicating co-localization of with and in these areas, respectively.

Conclusion: Immune-related genes and immune cells are intimately related to AMI. Constructing different ML models based on these biomarkers could be a valuable approach to diagnosing AMI in clinical practice.
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http://dx.doi.org/10.2147/JIR.S360498DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9174022PMC
June 2022
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