Publications by authors named "Ting Wang"

3,070 Publications

  • Page 1 of 1

Effects of Ultrashort Wave Therapy on Inflammation and Macrophage Polarization after Acute Lung Injury in Rats.

Bioelectromagnetics 2021 Jun 15. Epub 2021 Jun 15.

Rehabilitation Medicine Center, The First Affiliated Hospital of University of South China, Hengyang, People's Republic of China.

Acute lung injury (ALI) features dysregulated pulmonary inflammation. Ultrashort waves (USWs) exert anti-inflammatory effects but no studies have evaluated their activity in ALI. Herein, we used an in vivo lipopolysaccharide (LPS)-induced ALI model to investigate whether the anti-inflammatory activity of USWs is mediated by altering the polarization of M1 to M2 macrophages. Twenty-four male Sprague-Dawley rats were randomly divided into control, untreated ALI, and ALI treated with USW groups (n = 8 in each group). ALI was induced by intratracheal LPS instillation. Rats in the USW group were treated for 15 min at 0, 4, and 8 h after a single LPS intratracheal instillation. Histopathologic examination, wet/dry lung weight ratio, enzyme-linked immunosorbent assay, quantitative real-time polymerase chain reaction, and western blot analyses were performed to evaluate the degree of lung injury and to determine macrophage phenotypes. Histopathologic examination disclosed attenuation of ALI, with reduced alveolar hemorrhage and neutrophilic infiltration in the USW group. Serum levels of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were significantly decreased after USW therapy. Moreover, the messenger RNA (mRNA) expressions of TNF-α and IL-1β were significantly decreased in the USW group, whereas the mRNA expression of Arginase 1 (Arg1) and the protein expression of mannose receptor significantly increased in comparison with the untreated ALI group. We conclude that USW therapy may attenuate inflammation in LPS-induced ALI through the modulation of macrophage polarization. © 2021 Bioelectromagnetics Society.
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http://dx.doi.org/10.1002/bem.22353DOI Listing
June 2021

Saponins from Panax japonicus alleviate HFD-induced impaired behaviors through inhibiting NLRP3 inflammasome to upregulate AMPA receptors.

Neurochem Int 2021 Jun 12:105098. Epub 2021 Jun 12.

Hubei Province Key Laboratory of Occupational Hazard Identification and Control, Wuhan University of Science and Technology, Wuhan, 430065, China; New Medicine Innovation and Development Institute, Department of Pharmacy, College of Medicine, Wuhan University of Science and Technology, Wuhan, 430065, China. Electronic address:

Obesity is characterized by a condition of low-grade chronic inflammation that facilitates development of numerous comorbidities and dysregulation of brain homeostasis. It is reported that obesity can lead to behavioral alterations such as cognitive decline and depression-like behaviors both in humans and rodents. Saponins from panax japonicus (SPJ) have been reported to exhibit anti-inflammatory action in mouse model of diet-induced obesity. We evaluated the neuroprotection of SPJ on high fat diet (HFD) induced impaired behaviors such as memory deficit and depressive-like behaviors, and explored the underlying mechanisms. 6-week male Balb/c mice were divided into normal control group (NC, 17% total calories from fat), HFD group (60% total calories from fat), and HFD treated with SPJ groups (orally gavaged with dosages of 15 mg/kg and 45 mg/kg), respectively. After treatment for 16 weeks, behavioral tests were performed to evaluate the cognition and depression-like behaviors of the mice. The underling mechanisms of SPJ on HFD-induced impaired behaviors were investigated through histopathological observation, western blot analysis and immunofluorescence. Our results showed that HFD-fed mice caused behavioral disorders, neuronal degeneration as well as elevated neuroinflammation, which was partly involved in NLRP3 inflammasome that finally resulted in decreased protein levels of AMPA receptors and down-regulated phosphorylated levels of CaMKII and CREB in cortex and hippocampus. All the above changes in cortex and hippocampus induced by HFD were mitigated by SPJ treatment. SPJ treatment alleviated HFD-induced recognitive impairment and depression-like behaviors of mice, which could be partly due to the capacity of SPJ to mitigate neuroinflammation through inhibition of NLRP3 inflammasome and upregulation of AMPA receptors signaling pathway.
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http://dx.doi.org/10.1016/j.neuint.2021.105098DOI Listing
June 2021

Dissecting the Flash Chemistry of Electrogenerated Reactive Intermediates by Microdroplet Fusion Mass Spectrometry.

Angew Chem Int Ed Engl 2021 Jun 15. Epub 2021 Jun 15.

Najing University, Chemistry, Xianlin Ave. 163, , 210023, Nanjing, CHINA.

A novel mass spectrometric method for probing the flash chemistry of electrogenerated reactive intermediates was developed based on rapid collision mixing of electrosprayed microdroplets by using a theta-glass capillary. The two individual microchannels of the theta-glass capillary are asymmetrically or symmetrically fabricated with a carbon bipolar electrode to produce intermediates in situ . Microdroplets containing the newly formed intermediates collide with those of the invoked reactants at sub-10 microsecond level, making it a powerful tool for exploring their ultrafast initial transformations. As a proof-of-concept, we present the identification of the key radical cation intermediate in the oxidative dimerization of 8-methyl-1,2,3,4-tetrahydroquinoline and also the first revealment of previously hidden nitrenium ion involved reaction pathway in the C-H/N-H cross-coupling between N,N'-dimethylaniline and phenothiazine.
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http://dx.doi.org/10.1002/anie.202106945DOI Listing
June 2021

PEG-Induced Controllable Thin-Thickness Gradient and Water Retention: A Simple Way to Programme Deformation of Hydrogel Actuators.

Macromol Rapid Commun 2021 Jun 15:e2000749. Epub 2021 Jun 15.

The Collaborative Innovation Center for Eco-Friendly and Fire-Safety Polymeric Materials(MoE), National Engineering Laboratory of Eco-Friendly Polymeric Materials (Sichuan), State Key Laboratory of Polymer Materials Engineering, College of Chemistry, Sichuan University, Chengdu, 610064, China.

Building the differential growth through the thickness is a promising and challenging approach to design the morphing structures of hydrogel actuators. Besides retaining the size of the hydrogel actuators under environmental stimuli still remains a big challenge. Herein, a facile and universal approach is developed to address both issues by introducing PEG during the polymerization of N-isopropylacrylamide (NIPAm) via one step method using asymmetric mold. Both composition gradient and pore gradient are obtained in micro level along the thickness direction of the final hydrogel, while thin-thickness gradient in macro level. The thickness gradient and water retention can be controllably adjusted by changing PEG concentration. The introduction of PEG effectively improves both responsive and non-shrunken performance by the interaction with PNIPAm. The resultant anisotropic PNIPAm/PEG hydrogel respond quickly and reach maximum deformation (360°) within 10 s at low temperature (40 °C). The various 3D shape and biomimetic movement can be programmed by simply controlling the PEG concentration and mold shape. This strategy can provide new insights into the design intelligent soft materials with 3D morphing for bioinspired and biomedical applications.
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http://dx.doi.org/10.1002/marc.202000749DOI Listing
June 2021

Characterization of hydrophobic interaction of galactomannan in aqueous solutions using fluorescence-based technique.

Carbohydr Polym 2021 Sep 8;267:118183. Epub 2021 May 8.

Key Laboratory of Chemistry and Engineering of Forest Products, State Ethnic Affairs Commission, Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning 530006, China.

Fluorescence probing was used to study hydrophobic interactions of galactomannan (GM) obtained from fenugreek gum (FG), guar gum (GG), and locust bean gum (LBG) at different M/G ratios. The I/I ratio of pyrene changed from 1.73 to 1.29, 1.22, and 1.29 for FG, GG and LBG, respectively, as the concentration of GM increased from 0.01 to 8.0 g/L at 30 °C. The critical aggregation concentration of FG, GG, and LBG increased from 1.04 to 3.84 g/L, 1.15 to 3.73 g/L, and 0.94 to 3.63 g/L, respectively, as temperature increased from 10 to 70 °C. Addition of NaSO and NaSCN increased the I/I ratio in dilute solution, but reduced it in semi-dilute solution, whereas adding urea reduced I/I in dilute solution but increased it in semi-dilute solution. These results indicated that the CAC of GM, polarity and number of hydrophobic microdomains were highly dependent on the M/G ratio and galactose distribution.
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http://dx.doi.org/10.1016/j.carbpol.2021.118183DOI Listing
September 2021

Interventional bronchoscopic therapy in adult patients with tracheobronchial schwannoma.

Ann Palliat Med 2021 Jun 10. Epub 2021 Jun 10.

Department of Pulmonary and Critical Care Medicine, Beijing Tiantan Hospital, Capital Medical University, Beijing, Chin.

Background: Tracheobronchial schwannomas are extremely rare tumors of neurogenic origin. Treatment includes surgery and interventional bronchoscopic therapy. Studies that have described interventional bronchoscopic therapy for tracheobronchial schwannoma have been reported in the published literature, but most of them are individual case reports, in which the long-term efficacy and recurrence are poorly understood. This study aimed to explore the feasibility, efficacy, and safety of interventional bronchoscopic therapy in adult patients with tracheobronchial schwannoma.

Methods: Patients with pathologically diagnosed tracheobronchial schwannoma between January 2007 and December 2020 who underwent interventional bronchoscopic therapy in a single center in China were retrospectively reviewed. The clinical features, interventional bronchoscopic therapy procedures, complications, and follow-up results were analyzed.

Results: A total of 7 patients (5 men and 2 women; median age, 56 years; age range, 26-63 years) were enrolled. Patients' symptoms were all significantly relieved after interventional bronchoscopic therapy, with mean American Thoracic Society Dyspnea Index score decreasing from 2.29±0.76 to 0.29±0.49 (t=6.481; P=0.001). No severe procedure-related complications were observed. Intraluminal tumors were found subsequently in three cases during a short-term follow-up (4 days to 1 month). One patient underwent surgery; thereafter got lost to follow-up, while the other 2 patients underwent a second endoscopic resection with good prognosis. Six patients received long-term follow-up (range, 2-12 years; median period, 6 years), with all patients being in stable conditions.

Conclusions: Interventional bronchoscopic therapy is an acceptable approach in the management of tracheobronchial schwannoma, and is a useful alternative to surgical resection, especially for those who are at a high risk of surgery or unwilling to undergo surgery. However, long-range bronchoscopic surveillance is necessary because of the possibility of tumor recurrence. Due to its benign nature, repeat endoscopic resection can still be considered after recurrence.
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http://dx.doi.org/10.21037/apm-21-630DOI Listing
June 2021

Distinguishing between benign and malignant breast lesions using diffusion weighted imaging and intravoxel incoherent motion: A systematic review and meta-analysis.

Eur J Radiol 2021 Jun 6;141:109809. Epub 2021 Jun 6.

Department of Radiology, Shaoxing People's Hospital (Shaoxing Hospital, Zhejiang University School of Medicine), Key Laboratory of Functional Molecular Imaging of Tumor and Interventional Diagnosis and Treatment of Shaoxing City, Shaoxing 312000, China. Electronic address:

Purpose: We sought to evaluate the diagnostic performance of diffusion weighted imaging (DWI) and intravoxel incoherent motion (IVIM) for distinguishing between benign and malignant breast tumors by performing a meta-analysis.

Methods: We comprehensively searched the electronic databases PubMed and Embase from January 2000 to April 2020 for studies in English. Studies were included if they reported the sensitivity and specificity for identifying benign and malignant breast lesions using DWI or IVIM. Studies were reviewed according to QUADAS-2. The data inhomogeneity and publication bias were also assessed. In order to explore the influence of different field strengths and different b values on diagnostic efficiency, we conducted subgroup analysis.

Results: We analyzed 79 studies, which included a total of 6294 patients with 4091 malignant lesions and 2793 benign lesions. Overall, the pooled sensitivity and specificity of ADC for detecting malignant breast tumors were 0.87 (0.86-0.88) and 0.80 (0.78-0.81), respectively. The PLR was 5.09 (4.16-6.24); the NLR was 0.15 (0.13-0.18); and the DOR was 38.95 (28.87-52.54). The AUC value was 0.9297. The highest performing parameter for IVIM was tissue diffusivity (D), and the pooled sensitivity and specificity was 0.85 (0.82-0.88) and 0.87(0.83-0.90), respectively; the PLR was 5.65 (3.91-8.18); the NLR was 0.17 (0.12-0.26); and the DOR was 38.44 (23.57-62.69). The AUC value was 0.9265. Most of parameters demonstrated considerable statistically significant heterogeneity (P < 0.05, I>50 %) except the pooled DOR, PLR of D and the pooled DOR and NLR of D*.

Conclusions: Our meta-analysis indicated that DWI and IVIM had high sensitivity and specificity in the differential diagnosis of breast lesions; and compared with DWI, IVIM could not further increase the diagnostic performance. There was no significant difference in diagnostic accuracy.
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http://dx.doi.org/10.1016/j.ejrad.2021.109809DOI Listing
June 2021

Sorption, Desorption and Mobility of Microencapsulated Chlorpyrifos in Two Typical Soils.

Arch Environ Contam Toxicol 2021 Jun 10. Epub 2021 Jun 10.

College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310029, China.

The sorption, desorption, and mobility of microencapsulated chlorpyrifos (CPF-MC) in two typical soils, namely, silt loam and sandy, were investigated in this study. Sorption/desorption experiments were carried out by using the batch equilibration method. Results showed that the sorption isotherms of CPF-MC and emulsifiable concentrate of chlorpyrifos (CPF-EC) in silt loamy soil were similar. However, a considerable difference was observed in the sorption isotherms of two chlorpyrifos (CPF) formulations in sandy soil. The amounts of CPF desorbed from two soils in four desorption steps decreased sequentially in CPF-EC treatments, while the desorbed amounts remained stable in CPF-MC treatments. Hence, the sorption/desorption processes of CPF-EC were mainly controlled by soil affinity to CPF. However, these processes of CPF-MC were affected by the release rate of CPF from capsules. The mobility of two CPF formulations in soil was estimated in vertical columns packed with soils. Results showed that there was leaching of CPF-EC in silt loam column, whereas CPF-MC was not vertically mobile in silt loam column under the same leaching conditions. However, in column with sandy soil, the percentage of CPF-MC leaching from the column was 86.54%, which was higher than the 73.75% that leached from the column in the treatment with CPF-EC.
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http://dx.doi.org/10.1007/s00244-021-00864-wDOI Listing
June 2021

Correction: Recovery of the self-cleaning property of silicon elastomers utilizing the concept of reversible coordination bonds.

Soft Matter 2021 Jun 9. Epub 2021 Jun 9.

State Key Laboratory of Environmental-friendly Energy Materials, Southwest University of Science and Technology, Mianyang 621010, China.

Correction for 'Recovery of the self-cleaning property of silicon elastomers utilizing the concept of reversible coordination bonds' by Yuxing Shan et al., Soft Matter, 2020, 16, 8473-8481, DOI: 10.1039/D0SM01264E.
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http://dx.doi.org/10.1039/d1sm90109eDOI Listing
June 2021

Poloxometalates-based Semi-flexible Metal-semiconductor Triboelectric Nanogenerators for Low Frequency and Small Amplitude Mechanical Energy Harvesting.

Chemistry 2021 Jun 7. Epub 2021 Jun 7.

Key Laboratory of Polyoxometalate and Reticular Material Chemistry of Ministry of Education, Department of Chemistry, Northeast Normal University, Ren Min Street No.5268, 130024, Changchun, CHINA.

The development of high-performance and low-cost durable triboelectric nanogenerators (TENGs) is essential for converting mechanical energy into electrical energy. Many organic polymer friction materials used widely have thermal stability problems , which makes TENGs with semiconductors as friction materials stand out. Here, we report a semi-flexible TENG based on metal and TiO 2 modified by polyoxometalates (POMs) as pure inorganic friction materials. Six different POMs are firstly selected to modify the friction materials of TENGs, and the output performance of TENGs with different POMs-modified semiconductors and different metals as friction materials are tested. Compared with the unmodified TENGs, the open-circuit voltage ( V OC ) of the optimal Ag-K 6 P 2 Mo 18 O 62 (P 2 Mo 18 )/TiO 2 TENG device is increased by more than 4 times, which is mainly attributed to the strong electron-accepting and storage capabilities of POMs. This study has demonstrated that TENGs modified by POMs have potential application prospects and provided a new method for increasing the electrical output of TENGs.
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http://dx.doi.org/10.1002/chem.202100719DOI Listing
June 2021

Cytological and morphology characteristics of natural microsporogenesis within .

Physiol Mol Biol Plants 2021 May 16;27(5):959-968. Epub 2021 May 16.

The College of Forestry, Central South University of Forestry and Technology, Hunan, 410004 China.

is believed to exhibit a complex intraspecific polyploidy phenomenon. Abnormal microsporogenesis can promote the formation of unreduced gametes in plants and lead to sexual polyploidy, so it is hypothesized that improper meiosis probably results in the formation of natural polyploidy in . In this study, based on the cytological observation of meiosis in pollen mother cells (PMCs), we found natural 2n pollen for the first time in , which may lead to the formation of natural polyploids by sexual polyploidization. Additionally, abnormal cytological behaviour during meiosis, including univalent chromosomes, extraequatorial chromosomes, early segregation, laggard chromosomes, chromosome stickiness, asynchronous meiosis and deviant cytokinesis (monad, dyads, triads), was observed, which could be the cause of 2n pollen formation. Moreover, we confirmed a relationship among the length-width ratio of flower buds, stylet length and microsporogenesis. This result suggested that we can immediately determine the microsporogenesis stages by phenotypic characteristics, which may be applicable to breeding advanced germplasm in .

Supplementary Information: The online version contains supplementary material available at 10.1007/s12298-021-01002-5.
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http://dx.doi.org/10.1007/s12298-021-01002-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8140029PMC
May 2021

Evaluating marginal genetic correlation of associated loci for complex diseases and traits between European and East Asian populations.

Hum Genet 2021 Jun 6. Epub 2021 Jun 6.

Department of Epidemiology and Biostatistics, School of Public Health, Xuzhou Medical University, Xuzhou, 221004, Jiangsu, China.

Genome-wide association studies (GWASs) have successfully identified a large amount of single-nucleotide polymorphisms associated with many complex phenotypes in diverse populations. However, a comprehensive understanding of the genetic correlation of associated loci of phenotypes across populations remains lacking and the extent to which associations discovered in one population can be generalized to other populations or can be utilized for trans-ethnic genetic prediction is also unclear. By leveraging summary statistics, we proposed MAGIC to evaluate the trans-ethnic marginal genetic correlation (r) of per-allele effect sizes for associated SNPs (P < 5E-8) under the framework of measurement error models. We confirmed the methodological advantage of MAGIC over general approaches through simulations and demonstrated its utility by analyzing 34 GWAS summary statistics of phenotypes from the East Asian (N = 254,373) and European (N = 1,220,901) populations. Among these phenotypes, r was estimated to range from 0.584 (se = 0.140) for breast cancer to 0.949 (se = 0.035) for age of menarche, with an average of 0.835 (se = 0.045). We also uncovered that the trans-ethnic genetic prediction accuracy for phenotypes in the target population would substantially become low when using associated SNPs identified in non-target populations, indicating that associations discovered in the one population cannot be simply generalized to another population and that the accuracy of trans-ethnic phenotype prediction is generally dissatisfactory. Overall, our study provides in-depth insight into trans-ethnic genetic correlation and prediction for complex phenotypes across diverse populations.
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http://dx.doi.org/10.1007/s00439-021-02299-8DOI Listing
June 2021

The S100 protein family in lung cancer.

Clin Chim Acta 2021 Jun 3;520:67-70. Epub 2021 Jun 3.

Department of Radiology, Xi'an People's Hospital (Xi'an No.4 Hospital), Xi'an 710004, China. Electronic address:

The S100 protein family is involved in the pathogenesis of several malignancies including lung cancer. Recent studies have shown that one member, S100A2, was over-expressed in advanced stage non-small cell lung cancer (NSCLC). Another, S100A6, demonstrated variable expression in different lung cancer subtypes. Research using NSCLC cell lines reported that SIX3 inhibited cell metastasis and proliferation via S100P down-regulation. This review represents an update on S100 proteins in lung cancer from 2017 to 2021 and includes the aforementioned as well as S100A4, S100A7, and S100B. Inconsistencies in mechanisms of action for S100A8/S100A9 are highlighted and a comprehensive evaluation of the most recent evidence for the S100 proteins in lung cancer is presented.
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http://dx.doi.org/10.1016/j.cca.2021.05.028DOI Listing
June 2021

Soil capacity of intercepting different rainfalls across subtropical plantation: Distinct effects of plant and soil properties.

Sci Total Environ 2021 Aug 16;784:147120. Epub 2021 Apr 16.

University of Calgary, Calgary T2N1N4, Canada.

Forest management practices play an important role in soil water conservation. However, the soil water-holding capacity and associated drivers under different management practices remain uncertain, especially when the precipitation varies substantially at the regional scale. Here, we used hydrogen stable isotope to explore the contribution of rainfall to soil water (CRSW) under light, moderate and heavy precipitation in Pinus massoniana plantations with multiple management practices (pure stand, mixed stand, understory removal, light-intensity thinning and high-intensity thinning) in subtropical China. We further used variation partitioning analysis and structural equation modeling to identify the dominant driver affecting CRSW. Our results showed that after light rainfall, the highest CRSW (28.7%) was found in the high-intensity thinning plantation. However, after heavy rainfall, the high-intensity thinning plantation received the lowest CRSW (43.3%), while the mixed stand showed the highest CRSW (67.1%). These results demonstrated that the mixed stand of P. massoniana had a stronger capacity for soil water conservation, whereas high-intensity thinning showed poorer capacity. Furthermore, our results revealed that plant properties (i.e., tree, root and litter biomass) were the dominant controls of the CRSW under light rainfall, while soil properties (i.e., bulk density, total porosity, field capacity) were the primary drivers under moderate and heavy rainfall, indicating that the determinants influencing plantation capacity for intercepting rainfall vary with different levels of precipitation. These results highlight the importance of the level of precipitation in determining the dominant driver of CRSW. More importantly, these results suggest that the mixed stand, rather than high-intensity thinning, was better forest management since the former has a higher capacity for intercepting heavy rainfall.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147120DOI Listing
August 2021

Diffusion of water and protein drug in 1,4-butanediol diglycidyl ether crosslinked galactomannan hydrogels and its correlation with the physicochemical properties.

Int J Biol Macromol 2021 Jun 2;183:1987-2000. Epub 2021 Jun 2.

Key Laboratory of Chemistry and Engineering of Forest Products, State Ethnic Affairs Commission, Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning 530006, China. Electronic address:

The aim of the present study was to obtain a better and safer galactomannan-based material for drug release applications. A novel epoxy-crosslinked galactomannan hydrogel (EGH) was prepared from guar gum using 1,4-butanediol diglycidyl ether as a crosslinking agent. The diffusion rate constant of water molecules in freeze-dried EGH positively correlated with water uptake/equilibrium swelling rate (WU/ESR), and the water molecules participated in Fickian diffusion. The ESR, WU/ESR, and bovine serum albumin (BSA) loading capacity of a customized EGH with a crosslinking density of 48.9% were 48.7 ± 0.15 g/g, 95.3%, and 56.4 mg/g, respectively. The release of BSA from freeze-dried EGH was affected by the WU/ESR and the pH; the release equilibrium time was ~40 h at pH 1.2, decreasing to ~24 h at pH 7.4. Furthermore, the cumulative release rate increased from 63.5% to 80.7% and the t decreased from 59 to 41 min upon changing from the acidic to basic pH. The release process conformed to the Ritger-Peppas and Hixson-Crowell models, and represented Fickian diffusion and chain relaxation. The EGH showed no cytotoxicity toward HeLa cells. Together, these results demonstrate the properties of a novel galactomannan-based hydrogel that can potentially be employed as a vehicle for drug delivery.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.05.195DOI Listing
June 2021

Large-area Piezoelectric Single Crystal Composites via 3D-printing Assisted Dice-and-insert Technology for Hydrophone Applications.

IEEE Trans Ultrason Ferroelectr Freq Control 2021 Jun 4;PP. Epub 2021 Jun 4.

Compared with Pb(Zr,Ti)O3 (PZT) ceramics, piezoelectric ceramic composites (PCCs), and piezoelectric polyvinylidene fluoride (PVDF) polymer, piezoelectric single crystal composites (PSCCs) are thought to be promising candidates for hydrophone applications because of their superior hydrostatic performance. However, due to the brittleness and small dimensions of single crystals, the preparation of large-area or conformal PSCCs is to be challenged. Herein, we prepared a large-area PSCC with dimensions of 50 mm × 50 mm × 5 mm using 3D-printing assisted dice-and-insert technology. The hydrostatic piezoelectric performances for PSCC were investigated using a quasi-static method. The hydrostatic figure-of-merit (HFOM) of PSCC is approximately 1469×10-15 m2/N, which is higher by 69.4% than that of PCC. Furthermore, PSCC shows advantages in the dielectric loss, frequency constant, electromechanical coupling coefficient and hydrostatic pressure stability. The results suggest that PSCCs have great potential in substantially improving the sensitivity of hydrophones. In addition, 3D-printing assisted dice-and-insert technology breaks through the restriction of as-grown piezoelectric crystal size so as to make it possible for the applications where large-scale piezoelectric composites are required.
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http://dx.doi.org/10.1109/TUFFC.2021.3085842DOI Listing
June 2021

Design, Synthesis, and Biological Evaluation of Novel Pyrimido[4,5-]indole Derivatives Against Gram-Negative Multidrug-Resistant Pathogens.

J Med Chem 2021 Jun 3. Epub 2021 Jun 3.

State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.

Due to the poor permeability across Gram-negative bacterial membranes and the troublesome bacterial efflux mechanism, only a few GyrB/ParE inhibitors with potent activity against Gram-negative pathogens have been reported. Among them, pyrimido[4,5-]indole derivatives represented by GP-1 demonstrated excellent broad-spectrum antibacterial activity against both Gram-positive and Gram-negative bacteria but were limited by hERG inhibition and poor pharmacokinetics profile. To improve their drug-like properties, we designed a series of novel pyrimido[4,5-]indole derivatives based on the tricyclic scaffold of GP-1 and the C-7 moiety of acorafloxacin. These efforts have culminated in the discovery of a promising compound with reduced hERG liability and an improved PK profile. Compound exhibited superior broad-spectrum antibacterial activity compared to GP-1, including a variety of clinical multidrug G pathogens, especially , and the efficacy was also demonstrated in a neutropenic mouse thigh model of infection with multidrug-resistant .
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http://dx.doi.org/10.1021/acs.jmedchem.1c00621DOI Listing
June 2021

Scalable Room-Temperature Synthesis of Highly Robust Ethane-Selective Metal-Organic Frameworks for Efficient Ethylene Purification.

J Am Chem Soc 2021 Jun 2;143(23):8654-8660. Epub 2021 Jun 2.

College of Chemistry, Nankai University, Tianjin 300071, P. R. China.

The development of new techniques and materials that can separate ethylene from ethane is highly relevant in modern applications. Although adsorption-based separation techniques using metal-organic frameworks (MOFs) have gained increasing attention, the relatively low stability (especially water resistance) and unscalable synthesis of MOFs severely limit their application in real industrial scenarios. Addressing these challenges, we rationally designed and synthesized two new CH-selective MOF adsorbents ( and ) with ultrahigh chemical and thermal stability, including water resistance. Attributed to the nonpolar/hydrophobic pore environments and appropriate pore apertures, the MOFs can capture C2 hydrocarbon gases at ambient conditions even in high humidity. The single-crystal structures of realized the direct visualization of adsorption sites of the gases. Both the single-crystal data and simulated data elucidate the mechanism of selective adsorption. Moreover, the possesses high CH adsorption capacity and high selectivity, allowing for efficient CH/CH separation, as verified by experimental breakthrough tests. Most importantly, and can be scalably synthesized through stirring at room temperature in minutes, which confers them with great potential for industrial application. This work offers new adsorbents that can address major chemical industrial challenges and provides an in-depth understanding of the gas binding sites in a visual manner.
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http://dx.doi.org/10.1021/jacs.1c02108DOI Listing
June 2021

Metabolic and transcriptional analysis of recombinant Saccharomyces?cerevisiae for xylose fermentation: a feasible and efficient approach.

IEEE J Biomed Health Inform 2021 Jun 2;PP. Epub 2021 Jun 2.

Lignocellulose is an abundant xylose-containing biomass found in agricultural wastes, and has arisen as a suitable alternative to fossil fuels for the production of bioethanol. Although Saccharomyces cerevisiae has been thoroughly used for the production of bioethanol, its potential to utilize lignocellulose remains poorly understood. In this work, xylose-metabolic genes of Pichia stipitis and Candida tropicalis, under the control of different promoters, were introduced into S. cerevisiae. RNA-seq analysis was use to examine the response of S. cerevisiae metabolism to the introduction of xylose-metabolic genes. The use of the PGK1 promoter to drive xylitol dehydrogenase (XDH) expression, instead of the TEF1 promoter, improved xylose utilization in ?XR-pXDH? strain by overexpressing xylose reductase (XR) and XDH from C. tropicalis, enhancing the production of xylitol (13.66 ? 0.54 g/L after 6 days fermentation). Overexpression of xylulokinase and XR/XDH from P. stipitis remarkably decreased xylitol accumulation (1.13 ? 0.06 g/L and 0.89 ? 0.04 g/L xylitol, respectively) and increased ethanol production (196.14% and 148.50% increases during the xylose utilization stage, respectively), in comparison with the results of XR-pXDH. This result may be produced due to the enhanced xylose transport, Embden?Meyerhof and pentose phosphate pathways, as well as alleviated oxidative stress. The low xylose consumption rate in these recombinant strains comparing with P. stipitis and C. tropicalis may be explained by the insufficient supplementation of NADPH and NAD+. The results obtained in this work provide new insights on the potential utilization of xylose using bioengineered S. cerevisiae strains.
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http://dx.doi.org/10.1109/JBHI.2021.3085313DOI Listing
June 2021

Caloric restriction ameliorates high-fat diet induced cognitive deficits through attenuating neuroinflammation the TREM2-PI3K/AKT signaling pathway.

Food Funct 2021 Jun 2. Epub 2021 Jun 2.

Academy of Nutrition and Health, Wuhan University of Science and Technology, Wuhan 430065, China. and Hubei Province Key Laboratory of Occupational Hazard Identification and Control, Wuhan University of Science and Technology, Wuhan 430065, China and New Medicine Innovation and Development Institute, Department of Pharmacy, College of Medicine, Wuhan University of Science and Technology, Wuhan 430065, China.

Prolonged high-fat diet (HFD) feeding impairs cognitive function in rodents. However, the mechanism of caloric restriction (CR) for remedying HFD-induced cognitive dysfunction remains elusive. In the present study, we investigated the effect of CR on HFD-induced cognitive dysfunction and its possible mechanism. BALB/c mice were fed with HFD for 16 weeks and subsequently subjected to CR for 12 weeks. After cognitive function was evaluated by behavioral tests such as Morris water maze and three-chamber paradigm tests, the mice were sacrificed. The prefrontal cortex and hippocampus were rapidly harvested and deposited at -80 °C. The neuroprotective mechanisms of CR on HFD-induced cognitive deficits were evaluated by histopathological and electron microscopy observations, western blotting and immunofluorescence. Compared with the normal control group, HFD mice exhibited obvious cognitive deficits, glucose tolerance impairment, neuronal degeneration and abnormalities of synaptic ultrastructure in the cortex and hippocampus. CR treatment improved cognitive dysfunction and histopathological changes as well as increased the cognition-related protein levels of PSD-95, synaptophysin and BDNF. Meanwhile, HFD increased the protein levels of pro-inflammatory factors including iNOS, COX-2 and IL-1β but decreased the protein levels of anti-inflammatory factors such as CD206, TGF-β, Ym-1 and Arg 1 in the prefrontal cortex and hippocampus, downregulated the protein levels of TREM2 and PI3K and decreased the phosphorylation level of AKT, which can be reversed by CR treatment. Therefore, our results indicated that CR ameliorated cognitive deficits of mice induced by a high-fat diet. The underlying mechanism is associated with the attenuation of the neuroinflammatory response mediated by the TREM2-PI3K/AKT signaling pathway.
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http://dx.doi.org/10.1039/d0fo02946gDOI Listing
June 2021

Toward a versatile flow technique: Development and application of reverse flow dual-injection analysis (rFDIA) for determining dissolved iron redox species and soluble reactive phosphorus in seawater.

Talanta 2021 Sep 14;232:122404. Epub 2021 Apr 14.

College of the Environment and Ecology, Xiamen University, Xiamen, China.

A versatile flow analyzer that extended the features of reverse flow injection analysis (rFIA) was developed in this study and named reverse flow dual-injection analysis (rFDIA). Compared with typical rFIA, the analyzer requires less reagent and is more environmentally friendly, which has two injection valves and two reagent loops for the accurate and successive injection of two reagents. With a 2-m long liquid waveguide capillary cell (LWCC) and a spectrophotometer, the analyzer was applied to underway determination of dissolved iron redox species in estuarine and coastal waters. Detection limits of 0.18 and 0.20 nmol L were achieved for Fe(II) and Fe(II + III), respectively and a linear dynamic range of 0.5-450 nmol L was obtained for both Fe(II) and Fe(II + III). The sample throughput for the simultaneous measurement of Fe(II) and Fe(II + III) was 12 h, and each analysis consumed only 8 mL sample, 520 μL ferrozine solution, and 260 μL ascorbic acid solution. The analyzer was also used to measure nanomolar amounts of soluble reactive phosphorus (SRP) in seawater. The detection limit and the linear dynamic range for the SRP assay were 0.5 nmol L and 1.5-850 nmol L. For SRP determination, the sample throughput was 20 h, and each analysis required 9 mL of sample, 130 μL of mixed reagent solution and 260 μL of ascorbic acid. The analytical results were reproducible, with a relative standard deviation of 1.4% (2.5 nmol L, n = 10), 2.1% (2.5 nmol L, n = 10), and 2.1% (10 nmol L, n = 11) for Fe(II), Fe(II + III), and SRP, respectively.
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http://dx.doi.org/10.1016/j.talanta.2021.122404DOI Listing
September 2021

Reduction of PVA Aerogel Flammability by Incorporation of an Alkaline Catalyst.

Gels 2021 May 8;7(2). Epub 2021 May 8.

Department of Macromolecular Science & Engineering, Case Western Reserve University, Cleveland, OH 44106, USA.

Sodium hydroxide was used as a base catalyst to reduce the flammability of poly(vinyl alcohol) (PVA) aerogels. The base-modified aerogels exhibited significantly enhanced compressive moduli, likely resulting in decreased gallery spacing and increased numbers of "struts" in their structures. The onset of decomposition temperature decreased for the PVA aerogels in the presence of the base, which appears to hinder the polymer pyrolysis process, leading instead to the facile formation of dense char. Cone calorimetry testing showed a dramatic decrease in heat release when the base was added. The results indicate that an unexpected base-catalyzed dehydration occurs at fire temperatures, which is the opposite of the chemistry normally observed under typical synthesis conditions.
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http://dx.doi.org/10.3390/gels7020057DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8162340PMC
May 2021

Pathologic T1 and T2 encapsulated invasive carcinomas arising from mucinous cystic neoplasms of the pancreas have favorable prognosis and might be treated conservatively.

J Pathol Clin Res 2021 Jun 1. Epub 2021 Jun 1.

Department of Pathology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, PR China.

Carcinoma arising from a mucinous cystic neoplasm (MCN) of the pancreas is termed MCN with associated invasive carcinoma (MCN-AIC) in the fifth WHO classification of digestive tumors (2019). The prognosis of this malignancy varies depending on the relationship of the invasive carcinoma to the cyst capsule, but limited data are available. This study identified 165 surgically resected MCNs including 15 MCN-AICs from a single center between 2008 and 2018 and analyzed their clinicopathologic features. The results confirmed that non-invasive MCNs were completely cured by surgery. All MCN-AICs showing an encapsulated invasion pattern (defined as invasive carcinoma limited to the ovarian-type stroma, cystic septa, and capsule) had an excellent prognosis with a 5-year survival rate of 100%, even when the size of the invasive component was up to stage T2. By contrast, MCN-AICs with extracapsular involvement had unfavorable clinical outcomes. Our study demonstrates that the pattern of invasion of MCN-AIC can predict patient prognosis. Pathologic stage T1 and T2 encapsulated MCN-AICs may be completely cured with surgical resection alone or when combined with postoperative chemotherapy.
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http://dx.doi.org/10.1002/cjp2.225DOI Listing
June 2021

Quercetin for COVID-19 and DENGUE co-infection: a potential therapeutic strategy of targeting critical host signal pathways triggered by SARS-CoV-2 and DENV.

Brief Bioinform 2021 May 31. Epub 2021 May 31.

First Clinical Medical School of the Guangzhou University of Chinese Medicine, 12 Airport Road, Guangzhou 510405, PR China.

Background: The clinical consequences of SARS-CoV-2 and DENGUE virus co-infection are not promising. However, their treatment options are currently unavailable. Current studies have shown that quercetin is both resistant to COVID-19 and DENGUE; this study aimed to evaluate the possible functional roles and underlying mechanisms of action of quercetin as a potential molecular candidate against COVID-19 and DENGUE co-infection.

Methods: We used a series of bioinformatics analyses to understand and characterize the biological functions, pharmacological targets and therapeutic mechanisms of quercetin in COVID-19 and DENGUE co-infection.

Results: We revealed the clinical characteristics of COVID-19 and DENGUE, including pathological mechanisms, key inflammatory pathways and possible methods of intervention, 60 overlapping targets related to the co-infection and the drug were identified, the protein-protein interaction (PPI) was constructed and TNFα, CCL-2 and CXCL8 could become potential drug targets. Furthermore, we disclosed the signaling pathways, biological functions and upstream pathway activity of quercetin in COVID-19 and DENGUE. The analysis indicated that quercetin could inhibit cytokines release, alleviate excessive immune responses and eliminate inflammation, through NF-κB, IL-17 and Toll-like receptor signaling pathway.

Conclusions: This study is the first to reveal quercetin as a pharmacological drug for COVID-19 and DENGUE co-infection. COVID-19 and DENGUE co-infection remain a potential threat to the world's public health system. Therefore, we need innovative thinking to provide admissible evidence for quercetin as a potential molecule drug for the treatment of COVID-19 and DENGUE, but the findings have not been verified in actual patients, so further clinical drug trials are needed.
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http://dx.doi.org/10.1093/bib/bbab199DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8195157PMC
May 2021

Hydrochemistry and nutrients determined the distribution of greenhouse gases in saline groundwater.

Environ Pollut 2021 May 18;286:117383. Epub 2021 May 18.

College of Environmental Sciences and Engineering, Peking University, Key Laboratory of Water and Sediment Sciences, Ministry of Education, Beijing, 100871, China; State Environmental Protection Key Laboratory of All Materials Flux in River Ecosystems, Beijing, 100871, China.

The geography patterns and generation mechanisms of greenhouse gases (GHGs) in groundwater, especially in saline groundwater, are critical but rarely studied. Herein, we investigated the GHGs distribution in an aquifer, located upstream of Baiyangdian Lake, China, with a distinctive salinity gradient. A total of 132 groundwater samples were collected from 44 new-constructed wells along the lateral dimensions, and analyzed for dissolved GHGs concentrations, physiochemical parameters, and isotopes. The results showed that the dissolved CO, CH and NO concentrations ranged from 9.47 to 79.3 mg/L, 1.05-56.9 μg/L, and 0.84-7.03 μg/L, respectively. The groundwater was supersaturated with GHGs with respect to atmospheric equilibrium, suggesting groundwater discharge as a potential source of GHGs emission. CO significantly decreased while CH and NO distinctively increased with the decline of total dissolved solids (TDS) concentration, illustrating an obvious spatial pattern in the GHGs distribution. The CO distributions mainly depended on the bicarbonate radical and TDS, indicating carbonate equilibrium as the main process involving in the CO generation. CH and NO was primarily generated through the methanogenesis and denitrification processes, respectively. Nutrients including SO and total organic carbon predominately shaped the CH distributions, while nitrate mainly governed the NO distributions. Our study highlights the important roles of hydrochemistry and nutrients in the GHGs generation and distributions, which provides a significant insight on managing the GHGs emissions from the saline groundwater.
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http://dx.doi.org/10.1016/j.envpol.2021.117383DOI Listing
May 2021

Virtual bronchoscopic navigation non-virtual bronchoscopic navigation assisted bronchoscopy for the diagnosis of peripheral pulmonary lesions: a systematic review and meta-analysis.

Ther Adv Respir Dis 2021 Jan-Dec;15:17534666211017048

Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Chongqing Medical University, No 1 Youyi Road, Yuzhong, Chongqing 400016, China.

Background: Image-guided bronchoscopy techniques such as virtual bronchoscopic navigation (VBN) has emerged as a means of assisting in the biopsy of peripheral pulmonary lesions. However, the role of VBN-assisted (VBNA) bronchoscopy in the diagnosing of peripheral pulmonary lesions (PPLs) has not been well established. This meta-analysis investigated the diagnostic yield of VBN-assisted non-VBN-assisted (NVBNA) bronchoscopy for PPLs.

Methods: PubMed, Embase, Cochrane library, and Web of Sciences databases were searched up to and including August 2020 to identify randomized controlled trials (RCTs) evaluating the performance of VBNA compared with an NVBNA group. Results were expressed as risk ratio (RR) or mean difference (MD) with accompanying 95% confidence interval (CI).

Results: Six RCTs with 1626 patients were included. The overall diagnostic rate was similar in the VBNA (74.17%) and NVBNA (69.51%) groups, with risk ratio of 1.07 (95% CI: 0.98-1.17). However, in the VBNA group, the total examination time was significantly shorter (MD = -3.94 min, 95% CI: -6.57 to -1.36;  = 0.003) than in the NVBNA group. VBNA had superior diagnostic yield than NVBNA for PPLs ⩽ 20 mm (RR = 1.18, 95% CI: 1.05-1.32). In addition, diagnostic yield according to nature of lesion, lesion location in the lung lobe, distance from the hilum, bronchus sign and complications were similar between VBNA and NVBNA groups.

Conclusion: VBNA bronchoscopy did not increase overall diagnostic yield in patients with PPLs compared with NVBNA bronchoscopy. The superiority of VBNA over NVBNA was evident among patients with PPLs ⩽ 20 mm. Future multicenter RCTs are needed for further investigation.
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http://dx.doi.org/10.1177/17534666211017048DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8172954PMC
May 2021

Network Pharmacology Prediction and Pharmacological Verification Mechanism of Yeju Jiangya Decoction on Hypertension.

Evid Based Complement Alternat Med 2021 10;2021:5579129. Epub 2021 May 10.

College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China.

Background: Yeju Jiangya decoction (CIF) is an herbal formula from traditional Chinese medicine (TCM) for the treatment of hypertension.

Materials And Methods: Based on the analysis of network pharmacology, combined with in animal experiments, the network pharmacology was used to explore the potential proteins and mechanisms of CIF against hypertension. The bioactive compounds of CIF were screened by using the platform, and the targets of hypertension and CIF were collected. Then, the Kyoto Encyclopedia of Genes and Genomes (KEGG) and protein-protein interaction network (PPI) core targets were carried out, and the useful proteins were found by molecular docking technology. Finally, we used N-nitro-L-arginine (L-NNA) induced hypertension model rats to confirm the effect and mechanism of CIF on hypertension.

Results: 14 bioactive compounds of CIF passed the virtual screening criteria, and 178 overlapping targets were identified as core targets of CIF against hypertension. The CIF-related target network with 178 nodes and 344 edges is constructed. The topological results show that quercetin and luteolin are the key components in the network. The key targets NOS3 (nitric oxide synthase 3) and NOS2 (nitric oxide synthase 2) were screened by the protein-protein interaction network. The analysis of target protein pathway enrichment showed that the accumulation pathway is related to the vascular structure of CIF regulation of hypertension. Further verification based on molecular docking results showed that NOS3 had the good binding ability with quercetin and luteolin. On the other hand, NOS3 has an important relationship with the composition of blood vessels. Furthermore, the animal experiment indicated that after the L-NNA-induced hypertension rat model was established, CIF intervention was given by gavage for 3 weeks, and it can decrease serum concentrations of endothelin-1 (ET-1) and thromboxane B2 (TXB), increase the expression of nitric oxide (NO) and prostacyclin 2 (PGI), and improve renal, cardiac, and aortic lesions. At the same time, it can reduce blood pressure and shorten vertigo time. Western blot (WB) and immunohistochemistry (IHC) analyses indicated that CIF may downregulate the expression of NOS3, guanylyl cyclase-alpha 1 (GC-1), guanylyl cyclase-alpha 2 (GC-2), and protein kinase CGMP-dependent 1 (PRKG1). These results suggest that CIF may play an antihypertensive role by inhibiting the activation of the NOS3/PRKG1 pathway.

Conclusions: The results of this study indicate that CIF has the ability to improve target organs, protect endothelial function, and reduce blood pressure and that CIF might be a potential therapeutic drug for the prevention of hypertension. It provides new insight into hypertension and the potential biological basis and mechanism for CIF clinical research.
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http://dx.doi.org/10.1155/2021/5579129DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8131144PMC
May 2021

Concentrations, optical properties and sources of humic-like substances (HULIS) in fine particulate matter in Xi'an, Northwest China.

Sci Total Environ 2021 May 21;789:147902. Epub 2021 May 21.

Institute of Inorganic and Analytical Chemistry, Johannes Gutenberg University Mainz, Duesbergweg 10-14, 55128 Mainz, Germany.

Humic-like substances (HULIS) are ubiquitous in the atmospheric environment, which affects both human health and climate. We present here the mass concentration and optical characteristics of HULIS isolated from aerosol samples collected in Xi'an, China. Both mass concentration and absorption coefficient (Abs) of HULIS show clear seasonal differences, with the highest average in winter (3.91 μgC m and 4.78 M m, respectively) and the lowest in summer (0.65 μgC m and 0.55 M m, respectively). The sources of HULIS_C and light absorption of HULIS were analyzed by positive matrix factorization (PMF) and four major sources were resolved, including secondary formation, biomass burning, coal burning, and vehicle emission. Our results show that secondary formation (i.e., gas-to-particle conversion from e.g., photochemical oxidation) was the major contributor to both HULIS_C (50%) and light absorption (55%) of HULIS in summer, biomass burning and coal burning were major sources of HULIS_C (~70%) and light absorption (~80%) of HULIS in winter. It is worth noting that biomass burning and coal burning had higher contribution to HULIS light absorption (47% in spring, 37% in summer, 73% in fall, and 77% in winter) than their corresponding contribution to HULIS_C concentration (41% in spring, 37% in summer, 54% in fall, and 69% in winter). However, vehicle emission had lower contribution to HULIS light absorption (26% in spring, 8% in summer, 18% in fall, and 11% in winter) than to HULIS_C concentration (24% in spring, 13% in summer, 28% in fall, and 18% in winter). These results suggest that HULIS from biomass burning and coal burning have higher light absorption ability than from vehicle emission.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147902DOI Listing
May 2021

LncRNA WDFY3-AS2 promotes cisplatin resistance and the cancer stem cell in ovarian cancer by regulating hsa-miR-139-5p/SDC4 axis.

Cancer Cell Int 2021 May 29;21(1):284. Epub 2021 May 29.

Department of Integrated Chinese and Western Medicine Oncology, The First Affiliated Hospital of Anhui Medical University, No. 218, Jixi Road, Hefei, 230022, Anhui, China.

Background: Ovarian cancer (OC) is a high-mortality gynecological cancer that is typically treated with cisplatin, although such treatment often results in chemoresistance. Ovarian cancer resistance is usually related to cell stemness. Herein, we explored the function of lncRNA WDFY3-AS2 in OC cell resistance to cisplatin (DDP).

Methods: Cisplatin resistant OC A2780 cell lines (A2780-DDP) were established by long-term exposure to cisplatin. CCK-8 assay were performed to evaluate the viability of A2780, and A2780-DDP cells. Quantitative RT-PCR was used to examine the expression of lncRNA WDFY3-AS2, miR-139-5p, and SDC4 in A2780-DDP cell lines. After treatment with cisplatin, cell apoptosis and CD44CD166-positive cells were measured by flow cytometry. The transwell assays were employed to measure the effect of WDFY3-AS2 on cell migration, and invasion. In addition, tumorsphere formation assay was used to enrich OC cancer stem cells (CSCs) from A2780-DDP cells. The expression of CSC markers (SOX2, OCT4, and Nanog) was detected by western blotting. The regulatory mechanism was confirmed by RNA pull down, and luciferase reporter assays. Furthermore, xenograft tumor in nude mice was used to assess the impact of WDFY3-AS2 on cisplatin resistance in OC in vivo.

Results: WDFY3-AS2 was highly expressed in OC A2780-DDP cells, and silencing WDFY3-AS2 significantly inhibited proliferation, migration and invasion but increased apoptosis in OC A2780-DDP cells. Additionally, WDFY3-AS2 significantly promoted the A2780-DDP cells tumorspheres. WDFY3-AS2 was predicted to impact OC by sponging miR-139-5p and regulating SDC4. The xenografts inoculated with A2780-DDP cells additionally confirmed that tumor growth in vivo was reduced by si-WDFY3-AS2 transfection. MiR-139-5p inhibitor or SDC4 overexpression could restore the suppressive influence of silenced WDFY3-AS2 on tumor growth.

Conclusions: Together, WDFY3-AS2 may lead to change of cisplatin resistance by the expression of miR-139-5p/SDC4 in the OC A2870-DDP cells both in vitro and in vivo. Our finding may provide a drug target for the drug resistance of OC.
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http://dx.doi.org/10.1186/s12935-021-01993-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164817PMC
May 2021