Publications by authors named "Ting Tang"

131 Publications

Clinical analysis of 11 cases of nocardiosis.

Open Med (Wars) 2021 8;16(1):610-617. Epub 2021 Apr 8.

Department of Hematology, Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetic and Gene Regulation, Sun Yat-Sen University, Sun Yat-Sen Memorial Hospital, Guangzhou, Guangdong 510120, People's Republic of China.

Nocardiosis is a rare, life-threatening, opportunistic, and suppurative infection. Its clinical manifestation lacks specificity, which makes early diagnosis difficult. A retrospective analysis of the clinical records of 11 patients with nocardiosis admitted to our hospital from January 2013 to November 2018 was conducted. All patients had at least one underlying disorder, such as an autoimmune disease (6/11), a blood malignancy (2/11), avascular necrosis of the femoral head (1/11), bronchiectasis (1/11), or pneumonia (1/11). The first-line treatment was trimethoprim-sulfamethoxazole (TMP-SMX); one or two additional antibiotics were given according to the drug-sensitive test. The median time from onset to treatment was 3 weeks (ranging from 1 to 9 weeks). The median duration of treatment after diagnosis was 20.5 weeks (ranging from 7 to 47 weeks). Eight patients were discharged and survived, and three patients died. This indicates that early use of TMP-SMX combined with sensitive antibiotics could improve the condition of patients and improve the cure rate (8/11). Clinically, it is necessary to consider the possibility of nocardiosis in patients with long-term use of immunosuppressants and poor response to treatment of common bacterial infections. Early diagnosis, timely treatment, and combination drug therapy are keys to improving the outcomes of patients with nocardiosis.
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http://dx.doi.org/10.1515/med-2020-0196DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8034244PMC
April 2021

The Hypothetical Inclusion Membrane Protein CPSIT_0846 Regulates Mitochondrial-Mediated Host Cell Apoptosis the ERK/JNK Signaling Pathway.

Front Cell Infect Microbiol 2021 26;11:607422. Epub 2021 Feb 26.

Department of Public Health Laboratory Sciences, College of Public Health, University of South China, Hengyang, China.

is an important zoonotic factor associated with human and animal atypical pneumonia. Resisting host cell apoptosis is central to sustaining infection . can secrete inclusion membrane proteins (Incs) that play important roles in their development cycle and pathogenesis. CPSIT_0846 is an Inc protein in identified by our team in previous work. In the current study, we investigated the regulatory role of CPSIT_0846 in HeLa cell apoptosis, and explored potential mechanisms. The results showed that HeLa cells treated with CPSIT_0846 contained fewer apoptotic bodies and exhibited a lower apoptotic rate than untreated cells either with Hoechst 33258 fluorescence staining or flow cytometry with or without induction by staurosporine (STS). CPSIT_0846 could increase the phosphorylation of the extracellular signal-regulated kinases 1/2 (ERK1/2) or stress-activated protein kinases/c-Jun amino-terminal kinases (SAPK/JNK) signaling pathways, and the Bcl-2 associated X protein (Bax)/B cell lymphoma 2 (Bcl-2) ratio, levels of cleaved caspase-3/9 and cleaved Poly-ADP-ribose polymerase (PARP) were significantly up-regulated following inhibition of ERK1/2 or SAPK/JNK pathways with U0126 or SP600125. After carbonyl cyanide 3-chlorophenylhydrazone (CCCP) treatment, the mitochondrial membrane potential (MMP) of cells was significantly decreased in control group, but stable in the CPSIT_0846 treated one, and less cytochrome c (Cyt.c) was released into the cytoplasm. Inhibition of the ERK1/2 or SAPK/JNK pathway significantly decreased the JC-1 red-green fluorescence signal, and promoted Cyt.c discharge into the cytoplasm in HeLa cells treated with CPSIT_0846. In conclusion, CPSIT_0846 can regulate mitochondrial pathway-mediated apoptosis in HeLa cells by activating the ERK/JNK signaling pathway.
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http://dx.doi.org/10.3389/fcimb.2021.607422DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7971157PMC
February 2021

A Prognostic Signature Based on Immunogenomic Profiling Offers Guidance for Esophageal Squamous Cell Cancer Treatment.

Front Oncol 2021 24;11:603634. Epub 2021 Feb 24.

Department of Radiation Oncology, The First Affiliated Hospital of China Medical University, Shenyang, China.

Our study aimed to develop an immune prognostic signature that could provide accurate guidance for the treatment of esophageal squamous cell cancer (ESCC). By implementing Single-Sample Gene Set Enrichment Analysis (ssGSEA), we established two ESCC subtypes (Immunity High and Immunity Low) in GSE53625 based on immune-genomic profiling of twenty-nine immune signature. We verified the reliability and reproducibility of this classification in the TCGA database. Immunity High could respond optimally to immunotherapy due to higher expression of immune checkpoints, including PD1, PDL1, CTLA4, and CD80. We used WGCNA analysis to explore the underlying regulatory mechanism of the Immunity High group. We further identified differentially expressed immune-related genes (CCR5, TSPAN2) in GSE53625 and constructed an independent two-gene prognostic signature we internally validated through calibration plots. We established that high-risk ESCC patients had worse overall survival (P=0.002, HR=2.03). Besides, high-risk ESCC patients had elevated levels of infiltrating follicle-helper T cells, naïve B cells, and macrophages as well as had overexpressed levels of some immune checkpoints, including B3H7, CTLA4, CD83, OX40L, and GEM. Moreover, through analyzing the Genomics of Drug Sensitivity in Cancer (GDSC) database, the high-risk group demonstrated drug resistance to some chemotherapy and targeted drugs such as paclitaxel, gefitinib, erlotinib, and lapatinib. Furthermore, we established a robust nomogram model to predict the clinical outcome in ESCC patients. Altogether, our proposed immune prognostic signature constitutes a clinically potential biomarker that will aid in evaluating ESCC outcomes and promote personalized treatment.
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http://dx.doi.org/10.3389/fonc.2021.603634DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7943886PMC
February 2021

Analysis of microRNA expression profiles in human bronchial epithelial cells infected by Chlamydia psittaci.

Microb Pathog 2021 Mar 6;154:104837. Epub 2021 Mar 6.

Department of public health laboratory sciences, College of Public Health, University of South China, Hengyang, China; Key Laboratory of Hengyang for Health Hazard Factors Inspection and Quarantine, Hengyang, China. Electronic address:

Background: Chlamydia psittaci is a pathogen of birds that can cause zoonotic disease in mammals including pneumonia in humans. MicroRNAs (miRNAs) are a class of small non-coding RNA fragments with a length of about 22 nt, which play an important role in regulating gene expression after transcription. Chlamydia infection can cause changes in host cell miRNA expression, but the potential biological function of miRNAs in C. psittaci infection and pathogenesis is not well understood.

Methods: Small RNA sequencing (sRNA-Seq) technology was used to characterise miRNA expression in human bronchial epithelial (HBE) cells after C. psittaci infection, and differentially expressed miRNAs were identified. Candidate target genes for these miRNAs were then functionally annotated by Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. The sRNA-Seq results were partially validated by quantitative real time polymerase chain reaction (qRT-PCR) and miRNA-target networks were constructed using visualization software.

Results: We identified 151 differentially expressed miRNAs (46 known miRNAs and 105 novel miRNAs) in C. psittaci-infected HBE cells, of which 140 were upregulated and 11 were downregulated. Of these, 17 known miRNAs were significantly upregulated and two were downregulated using P < 0.05 and |logFoldChange|>1.5 as threshold criteria. GO enrichment results showed that the predicted targets of these differentially expressed miRNAs were mainly involved in transcriptional regulation and ATP binding. KEGG pathway analysis suggested that the candidate target genes were involved in several important signaling pathways such as MAPK, ErbB, cGMP-PKG, cAMP, mTOR, GNRH, oxytocin, PI3K-Akt and AMPK, which are primarily related to biological processes such as transcription and signal transduction. The qRT-PCR results for miR-2116-3p, miR-3195, miR-663a, miR-10401-5p, miR-124-3p, miR-184, miR-744-5p and hsa-miR-514b-5p were consistent with the sRNA-Seq data.

Conclusions: A large amount of miRNA expression profile data relating to C. psittaci infection was obtained, which provides a useful experimental and theoretical basis for further understanding the pathogenic mechanisms of C. psittaci infection.
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http://dx.doi.org/10.1016/j.micpath.2021.104837DOI Listing
March 2021

Kaempferide improves glycolipid metabolism disorder by activating PPARγ in high-fat-diet-fed mice.

Life Sci 2021 Apr 27;270:119133. Epub 2021 Jan 27.

Department of Cardiology, The First Affiliated Hospital of Chongqing Medical University, No.1 Youyi Road, Yuanjiagang, Yuzhong District, Chongqing 400042, China. Electronic address:

Aims: Kaempferide (Ka, 3,5,7-trihydroxy-4'-methoxyflavone), an active ingredient of Tagetes erecta L., has been demonstrated to possess many pharmacological effects, including antioxidant, anti-inflammation, anticancer and antihypertension in previous study. However, there is no evidence of Ka on metabolic disorder in former studies. This study investigated the effects of Ka on glycolipid metabolism and explored the underlying mechanisms of action in vivo and vitro.

Materials And Methods: The mouse model of glycolipid metabolism disorder was induced by high-fat diet (HFD). The effects of Ka were evaluated on bodyweight, lipid metabolism and glucose metabolism. Hypolipidemic effect was examined by blood sample analysis. The hypoglycemic effect was detected by several indicators, like blood glucose, serum insulin, HOMA index and intraperitoneal glucose tolerance tests (IPGTT). The signaling pathways of lipid metabolism (PPARγ/LXRα/ABCA1) and glucose metabolism (PPARγ/PI3K/AKT) were evaluated using Real-Time PCR and Western blot. The primary culture of hepatocyte was prepared to confirm the target of Ka by co-culturing with PPARγ agonist or inhibitor.

Key Findings: The HFD mice developed obesity, hyperlipidemia, hyperglycemia and insulin resistance. Administration of Ka at a dose of 10 mg/kg.BW for 16 weeks effectively attenuated these changes. Further studies revealed the hypolipidemic and hypoglycemic effects of Ka depended on the activation of PPARγ/LXRα/ABCA1 and PPARγ/PI3K/AKT pathways, respectively. The primary hepatocyte test, co-cultured with PPARγ agonists or inhibitors, further confirmed the above signaling pathway and key protein.

Significance: These results suggested that Ka played an important role in improving glycolipid metabolism disorder. These favorable effects were causally associated with anti-obesity. The underlying mechanisms might have to do with the activation of the PPARγ and its downstream signaling pathway. Our study helped to understand the pharmacological actions of Ka, and played a role for Ka in the effective treatment of obesity, diabetes, nonalcoholic hepatitis and other metabolic diseases.
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http://dx.doi.org/10.1016/j.lfs.2021.119133DOI Listing
April 2021

Application of serum markers in diagnosis and staging of ovarian endometriosis.

J Obstet Gynaecol Res 2021 Apr 14;47(4):1441-1450. Epub 2021 Jan 14.

Department of Assisted Reproduction, Ganzhou People's Hospital of Jiangxi Province, Ganzhou, China.

Aims: Laparoscopic surgery is widely used for diagnosing ovarian endometriosis but it has medical risks. This study explored the application of blood indicators in diagnosis and staging of ovarian endometriosis, aiming to develop a noninvasive diagnostic method.

Methods: A total of 190 ovarian endometriosis patients were included in observation group, among these participants, 77 patients among them were stages I-II, and the rest 113 patients were stages III-IV, and a total of 103 healthy women as control group. Serum biochemical indexes, tumor markers, and cytokines levels in two groups were used for the diagnosis and staging of the disease. Area under the receiver operating characteristic (ROC) curve (AUC) predicted the value of individual and joint tests for indicators.

Results: Biochemical indexes, namely, alkaline phosphatase (ALP), total protein (TP), and glucose (Glu) could distinguish patients from normal women; and that ALP and Glu could indicate disease staging. In tumor markers, alpha fetoprotein (AFP), carcinoembryonic antigen (CA) 125, CA199 and human epididymis protein 4 (HE4) helped to diagnose endometriosis; CA125, HE4, and cytokeratin 19 fragment (CYFRA21-1) could differentiate stages. In cytokines, vascular endothelial growth factor (VEGF), tumor necrosis factor (TNF)-α, interleukin (IL)-6, soluble fms-like tyrosine kinase receptor 1 (sflt-1), monocyte chemoattractant protein (MCP)-1 therefore, have values to diagnose endometriosis; VEGF, TNF-α, IL-6, and sflt-1 helped to differentiate disease staging.

Conclusion: Serological indicators in ovarian endometriosis patients were different from healthy women, which were of certain differential values in diagnosis and disease staging. The current study provided a novel strategy for endometriosis diagnosis.
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http://dx.doi.org/10.1111/jog.14654DOI Listing
April 2021

Inhibition of Cathepsin S Induces Mitochondrial Apoptosis in Glioblastoma Cell Lines Through Mitochondrial Stress and Autophagosome Accumulation.

Front Oncol 2020 23;10:516746. Epub 2020 Dec 23.

Department of Neurosurgery, Jinling Hospital, Nanjing, China.

Cathepsin S (CTSS), a lysosomal cysteine protease, is overexpressed in various cancers, including glioblastoma (GB). A high level of CTSS is associated with tumor progression and poor outcome in GB. However, the underlying mechanisms of its role in the biological characteristics of G5B remain to be elucidated. Here, we uncovered a potential role of CTSS in the lysosomes and mitochondria of GB cells (GBCs). Downregulation of CTSS in GBCs could increase the expression of autophagy-related proteins; however, there was no significant change in p62, suggesting autophagy blockade. Moreover, inhibition of CTSS increased the expression of mitochondrial calcium uniporter (MCU) and enhanced mitochondrial Ca uptake ability, causing mitochondrial Ca overload, the generation of copious reactive oxygen species (ROS) and eventual mitochondrial apoptosis. Additionally, elevated damage to mitochondria exacerbated the burden of autophagy. Finally, we found that silence of MCU could alleviate the inhibition of CTSS-induced autophagosome accumulation and mitochondrial stress. Collectively, these results demonstrate that CTSS plays an important role in the process of autophagic flux and mitochondrial functions in GBCs.
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http://dx.doi.org/10.3389/fonc.2020.516746DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7787074PMC
December 2020

Smarcd1 Inhibits the Malignant Phenotypes of Human Glioblastoma Cells via Crosstalk with Notch1.

Mol Neurobiol 2021 Apr 14;58(4):1438-1452. Epub 2020 Nov 14.

Department of Neurosurgery, Jinling Hospital, Medical School of Nanjing University, 305 East Zhongshan Road, Nanjing, 210002, Jiangsu, People's Republic of China.

Smarcd1 is a component of an evolutionary conserved chromatin remodeling complex-SWI/SNF, which is involved in transcription factor recruitment, DNA replication, recombination, and repair. Suppression of the SWI/SNF complex required for cellular differentiation and gene regulation may be inducible for cell proliferation and tumorigenicity. However, the inhibitory role of Smarcd1 in human glioblastoma cells has not been well illustrated. Both U87 and U251 human glioblastoma cell lines were employed in the present study. The lentivirus-mediated gene knockdown and overexpression approach was conducted to determine the function of Smarcd1. The protein levels were tested by western blot, and the relative mRNA contents were detected by quantitative real-time PCR. Cell viability was tested by CCK-8 and colony-forming assay. Transwell assays were utilized to evaluate the motility and invasive ability. Flow cytometry was employed to analyze cell cycle and apoptosis. SPSS software was used for statistical analysis. Low expression of Smarcd1 was observed in glioblastoma cell lines and in patients with high-grade glioma. Importantly, the depletion of Smarcd1 promoted cell proliferation, invasion, and chemoresistance, whereas enhanced expression of Smarcd1 inhibited tumor-malignant phenotypes. Mechanistic research demonstrated that overexpression of Smarcd1 decreased the expression of Notch1, while knockdown of Notch1 increased the expression of Smarcd1 through Hes1 suppression. Hence, the crosstalk between Smarcd1 and Notch1, which formed a feedback loop, was crucial in regulation of glioblastoma malignant phenotypes. Furthermore, targeting Smarcd1 could be a potential strategy for human glioblastoma treatment.
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http://dx.doi.org/10.1007/s12035-020-02190-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7932991PMC
April 2021

lncRNA TPTEP1 inhibits stemness and radioresistance of glioma through miR‑106a‑5p‑mediated P38 MAPK signaling.

Mol Med Rep 2020 Dec 28;22(6):4857-4867. Epub 2020 Sep 28.

Department of Spine Surgery, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian 362000, P.R. China.

Glioma is diagnosed as the most common intracranial malignant tumor. Cancer stem cells determine stemness and radioresistance, and may facilitate glioma recurrence. The present study aimed to investigate whether the long non‑coding RNA (lncRNA) transmembrane phosphatase with tensin homology pseudogene 1 (TPTEP1) regulated cell stemness and radioresistance of glioma, and determine the underlying molecular mechanism of TPTEP1 in the modulation of glioma progression. Cell and molecular biology techniques were applied for investigating the role of TPTEP1 in glioma cell lines, animal model, and clinical samples. The results demonstrated that TPTEP1 attenuated stemness and radioresistance of glioma both in vitro and in vivo. In addition, TPTEP1 augmented MAPK14 expression by competitively interacting with microRNA (miR)‑106a‑5p, thus activating the P38 MAPK signaling pathway, and suppressing glioma stemness and radioresistance. TPTEP1 functionally bound to miR‑106a‑5p, which formed a reciprocal regulatory loop to stimulate the P38 MAPK signaling pathway. Low TPTEP1 expression levels were detected in high‑grade glioma tissues compared with low‑grade glioma tissues, and were positively associated with poor prognosis of patients with glioma. Furthermore, analysis using data from The Cancer Genome Atlas database confirmed the molecular mechanism and biological significance of dysregulation of TPTEP1 in glioma progression. Taken together, the results of the present study suggest that TPTEP1 may be applied as a diagnostic and prognostic indicator for glioma, and may be an alternative target for the treatment of glioma.
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http://dx.doi.org/10.3892/mmr.2020.11542DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7646932PMC
December 2020

Polymer Ligands Derived from Jute Fiber for Heavy Metal Removal from Electroplating Wastewater.

Polymers (Basel) 2020 Oct 29;12(11). Epub 2020 Oct 29.

Faculty of Science and Nature Resources, University Sabah Malaysia, Kota Kinabalu 88400, Malaysia.

Industrial operations, domestic and agricultural activities worldwide have had major problems with various contaminants caused by environmental pollution. Heavy metal pollution in wastewater also a prominent issue; therefore, a well built and economical treatment technology is demanded for pollution-free wastewater. The present work emphasized pure cellulose extracted from jute fiber and further modification was performed by a free radical grafting reaction, which resulted in poly(methyl acrylate) (PMA)-grafted cellulose and poly(acrylonitrile)-grafted cellulose. Subsequently, poly(hydroxamic acid) and poly(amidoxime) ligands were prepared from the PMA-grafted cellulose and PAN-grafted cellulose, respectively. An adsorption study was performed using the desired ligands with heavy metals such as copper, cobalt, chromium and nickel ions. The binding capacity (q) with copper ions for poly(hydroxamic acid) is 352 mg g whereas q for poly(amidoxime) ligand it was exhibited as 310 mg g. Other metal ions (chromium, cobalt and nickel) show significance binding properties at pH 6. The Langmuir and Freundlich isotherm study was also performed. The Freundlich isotherm model showed good correlation coefficients for all metal ions, indicating that multiple-layers adsorption was occurred by the polymer ligands. The reusability was evaluated and the adsorbents can be reused for 7 cycles without significant loss of removal performance. Both ligands showed outstanding metals removal capacity from the industrial wastewater as such 98% of copper can be removed from electroplating wastewater and other metals (cobalt, chromium, nickel and lead) can also be removed up to 90%.
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http://dx.doi.org/10.3390/polym12112521DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7692318PMC
October 2020

Construction of Cognitive Maps to Improve Reading Performance by Text Signaling: Reading Text on Paper Compared to on Screen.

Front Psychol 2020 30;11:571957. Epub 2020 Sep 30.

Department of Psychology, School of Educational Science, Hunan Normal University, Changsha, China.

Reading text from a screen has been shown to be less effective compared with reading text from paper. Various signals may provide both background information and navigational cues, and may promote the construction of cognitive maps during on-screen reading, thus improving reading performance. This study randomly divided 75 college students into a paper reading group and an on-screen reading group. Both groups were tested for navigation and reading comprehension in response to three different forms of signaling (plain text, physical signaling, and verbal signaling). The results showed that when plain text was presented, the navigation and comprehension scores of the paper reading group were significantly higher than those of the on-screen reading group. However, no significant difference was found between both groups under signaling conditions. The navigation and comprehension scores of both groups were significantly higher under signaling conditions than under plain text. Moreover, the comprehension score of the on-screen reading group under physical signaling was significantly higher than that under verbal signaling. This research suggested that signals help to construct cognitive maps and effectively improve reading performance. Besides, physical signaling, such as underlining and bold formatting, is more effective for on-screen reading. The present study provides a practical and effective approach for improving on-screen reading based on cognitive map theory.
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http://dx.doi.org/10.3389/fpsyg.2020.571957DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7554618PMC
September 2020

Effect of gibberellin-sensitive Rht18 and gibberellin-insensitive Rht-D1b dwarfing genes on vegetative and reproductive growth in bread wheat.

J Exp Bot 2021 Feb;72(2):445-458

CSIRO Agriculture and Food, Canberra, ACT, Australia.

Gibberellin (GA)-insensitive dwarfing genes Rht-B1b and Rht-D1b that are responsible for the 'Green Revolution' have been remarkably successful in wheat improvement globally. However, these alleles result in shorter coleoptiles and reduced vigour, and hence poor establishment and growth in some environments. Rht18, on the other hand, is a GA-sensitive, dominant gene with potential to overcome some of the early growth limitations associated with Rht-B1b and Rht-D1b. We assessed progeny from both a biparental and a backcross population that contained tall, single dwarf, and double dwarf lines, to determine whether Rht18 differs from Rht-D1b and hence verify its value in wheat improvement. Progeny with Rht18 had an almost identical height to lines with Rht-D1b, and both were ~26% shorter than the tall lines, with the double dwarf 13% shorter again. However, coleoptile length of Rht18 was 42% longer than that of Rht-D1b. We detected no differences in time to terminal spikelet and anthesis, and few differences in stem or spike growth. Both dwarfing genes diverted more dry matter to the spike than tall lines from prior to heading. No differences were detected between Rht18 and Rht-D1b that could prevent the adoption of Rht18 in wheat breeding to overcome some of the limitations associated with the 'Green Revolution' genes.
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http://dx.doi.org/10.1093/jxb/eraa481DOI Listing
February 2021

Self-reported reasons for treatment nonadherence in chronic obstructive pulmonary disease (COPD) patients: a 24-week prospective cohort study in China.

Ann Palliat Med 2020 Sep;9(5):3495-3505

Institute of Respiratory Disease, Xinqiao Hospital, Third Military Medical University, Chongqing, China.

Background: Adherence to medication plays an important role in the control of disease progression and treatment costs for patients with chronic obstructive pulmonary disease (COPD). Existing studies are mostly retrospective studies based on local medical insurance databases and prescription records and therefore cannot analyze or determine the causes of poor adherence in COPD patients.

Methods: This is a prospective study that enrolled 1,223 COPD patients, including 929 newly diagnosed patients and 294 previously diagnosed patients. Telephone follow-up was conducted to administer a questionnaire on treatment adherence and collect detailed information on the prescriptions and the actual use of medications for COPD. The follow-up period was 24 weeks.

Results: COPD patients had adherence rates of 74.65%, 49.63%, and 33.61% at 4-, 12-, and 24-week telephone follow-ups, respectively. Cox regression showed that women, newly diagnosed patients, patients with a mild Global Initiative for Chronic Obstructive Lung Disease (GOLD) stage, and patients with fewer routes of administration had poorer adherence. According to the subgroup analysis of the diagnostic status, the overall adherence of the newly diagnosed patients was poorer than that of the previously diagnosed patients. Among the causes of nonadherence, drug stoppage after remission (27.2%) was the most common cause, followed by economic factors (21.5%) and poor efficacy (21.1%).

Conclusions: This is the first study focusing on the adherence behavior of newly diagnosed COPD patients and the first large-scale clinical study on the Chinese population. Overall, sex, diagnostic status (newly diagnosed and previously diagnosed), GOLD stage, and routes of administration were the four major factors that affected the adherence of COPD patients.
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http://dx.doi.org/10.21037/apm-20-1741DOI Listing
September 2020

Nutritional Status and Symptoms in Preschool Children With Autism Spectrum Disorder: A Two-Center Comparative Study in Chongqing and Hainan Province, China.

Front Pediatr 2020 3;8:469. Epub 2020 Sep 3.

Children's Nutrition Research Center, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children's Hospital of Chongqing Medical University, Chongqing, China.

The study aimed to compare the nutritional status and symptoms of preschool children with autism spectrum disorder (ASD) from two regions of China, and to analyze the association between nutritional status and symptoms of ASD. In this cross-sectional study, 738 ASD children and 302 typically developing children (TD) were recruited from Chongqing and Hainan of China. Symptoms of ASD children were evaluated with the Autism Behavior Checklist (ABC), Social Responsiveness Scale (SRS), and Childhood Autism Rating Scale (CARS). Neurodevelopment of ASD children was assessed with the Gesell Developmental Scale (GDS). Nutritional status was evaluated by anthropometric measures, biochemical detection of micronutrients, and providing questionnaire and food frequency questionnaire (FFQ) to caregivers. Comparing ASD children with local TD children, ASD children consumed fewer whole grains, milk and dairy products, beans and soy products, vegetables, and fruits than local TD children in both regions. The serum concentrations of folate, vitamin B12 (VB12), and vitamin D (VD) were consistently lower in ASD children in both regions. Comparing the ASD children between the two regions, the ASD children in Chongqing had significantly higher mean scores of CARS, SRS, and ABC than those in Hainan. The ASD children in Chongqing consumed fewer whole grains, seafood, and fruits than those in Hainan. The serum concentrations of ferritin, vitamin A (VA), VB12, and VD were reduced in the ASD children of Chongqing than those in Hainan, and the ASD children in Chongqing had higher deficiency rates of zinc, ferritin, VA, and VD than those in Hainan. The serum levels of VA, VD, and folate showed a negative association with symptom scores of ASD children. VD and zinc levels had a positive association with the GDS scores of ASD children. ASD children exhibit a higher risk of nutrient deficiencies than neurotypical children, and there are regional differences in the nutritional status of ASD children. Micronutrients VA, VD, folate, and zinc levels were correlated with symptoms and development of ASD children. Therefore, it is essential to provide detailed nutrition evaluation and individualized nutrition interventions for ASD children from different backgrounds.
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http://dx.doi.org/10.3389/fped.2020.00469DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7494825PMC
September 2020

CircARHGAP12 promotes nasopharyngeal carcinoma migration and invasion via ezrin-mediated cytoskeletal remodeling.

Cancer Lett 2021 Jan 12;496:41-56. Epub 2020 Sep 12.

NHC Key Laboratory of Carcinogenesis, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan, PR China; The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medicine Sciences, Central South University, Changsha, Hunan, PR China; Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Disease Genome Research Center, The Third Xiangya Hospital, Central South University, Changsha, Hunan, PR China. Electronic address:

An increasing number of studies have shown that circular RNAs (circRNAs) play important roles in malignant tumor initiation and progression; however, many circRNAs are yet unidentified, and the role of circRNAs in nasopharyngeal carcinoma (NPC) is unclear. Using RNA sequencing, we discovered a novel circRNA, termed circARHGAP12, that was processed from the pre-mRNA of the ARHGAP12 gene. CircARHGAP12 was significantly upregulated in NPC tissues and cell lines and promoted NPC cell migration and invasion. Overexpression or knockdown experiments revealed that circARHGAP12 regulates the expression of cytoskeletal remodeling-related proteins EZR, TPM3, and RhoA. CircARHGAP12 was found to bind directly to the 3' UTR of EZR mRNA and promote its stability; moreover, EZR protein interacted with TPM3 and RhoA and formed a complex to promote NPC cell invasion and metastasis. This study identified the novel circRNA circARHGAP12, characterized its biological function and mechanism, and increased our understanding of circRNAs in NPC pathogenesis. In particular, circARHGAP12 was found to promote the malignant biological phenotype of NPC via cytoskeletal remodeling, thus providing a clue for targeted therapy of NPC.
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http://dx.doi.org/10.1016/j.canlet.2020.09.006DOI Listing
January 2021

Neuropsychological and neuroimaging assessments of early cognitive impairment in patients after mild ischemic stroke and transient ischemic attack.

J Integr Neurosci 2020 Jun;19(2):313-319

Department of Neurology, The Second Affiliated Hospital of Fujian Medical University. Quanzhou, Fujian Province, 362000, P. R. China.

This study aimed to identify markers of early cognitive impairment after acute mild ischemic cerebrovascular disease. To further explore the relationship between neuroimaging markers of vascular and neurodegenerative injuries and post-stroke cognitive impairment, 86 patients with transient ischemic attack/acute mild ischemic stroke were recruited. Demographic information, clinical data, stroke scale scores (Modified Rankin Scale, National Institutes of Health Stroke Scale), and neuroimaging parameters (medial temporal lobe atrophy, global cortical atrophy, white matter hyperintensities, location and number of acute infarcts) were collected. All participants underwent neuropsychological evaluation at the time of discharge. The neurocognitive assessment was conducted using the Montreal Cognitive Assessment-Basic and Trail-Making Test A. It was found that low Montreal Cognitive Assessment-Basic scores were associated with global cortical atrophy and lower education levels. The completion time on the Trail-Making Test A was significantly correlated with medial temporal lobe atrophy and less education. It is concluded that global cortical atrophy and lower education levels can be used as rapid indicators of early cognitive impairment in patients after a transient ischemic attack and acute mild ischemic stroke. Medial temporal lobe atrophy also appears to be associated with mental processing speed in patients after a transient ischemic attack and acute mild ischemic stroke.
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http://dx.doi.org/10.31083/j.jin.2020.02.14DOI Listing
June 2020

Construction of a meroterpenoid-like compound collection by precursor-assisted biosynthesis.

Org Biomol Chem 2020 Aug;18(30):5850-5856

State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.

Natural products (NPs) and their derivatives play a pivotal role in drug discovery due to their complexity and diversity. The strategies to rapidly generate NP-like compounds offer unique opportunities to access bioactive compounds. Here we present a new approach, precursor-assisted biosynthesis (PAB), for the creation of NP-like compounds by combination of artificial supplementation of common precursors and divergent post-modifications of precursor-deficient fungi. This method was applied to construct a meroterpenoid-like compound collection containing 43 compounds with diverse molecular scaffolds. Extensive bioactive screening of the collection revealed novel STING (stimulator of interferon genes) inhibitors, cytotoxic and antifungal compounds. This result indicates that PAB is an effective methodology for producing compound collections for the purpose of drug discovery.
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http://dx.doi.org/10.1039/d0ob01235aDOI Listing
August 2020

The anti-insulin resistance effect of scutellarin may be related to antioxidant stress and AMPKα activation in diabetic mice.

Obes Res Clin Pract 2020 Jul - Aug;14(4):368-374. Epub 2020 Jul 4.

Department of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, People's Republic of China. Electronic address:

Aims: Scutellarein (Sc), a natural compound and an active ingredient of Erigeronbrevis-capus (Vant.), shows anti-obesity, anti-inflammation and lipid-lowering properties in our previous study. However, no previous in vivo and vitro has been conducted to assess the effects of Sc in insulin resistance (IR). This study investigated the effects of Sc on IR and oxidative stress and explored the underlying mechanisms of action in vivo and vitro.

Material And Method: A well-established mouse model of IR, induced by high-fat diet (HFD) feeding, was applied in this study. The effects of Sc were evaluated on obesity, glycometabolism disorder and oxidative stress. The anti-IR effect was assessed using blood glucose, serum insulin, HOMA index, intraperitoneal glucose tolerance tests (IPGTT), intraperitoneal insulin tolerance tests (IPITT), and glucose-regulating enzyme activity. The insulin signaling pathways and AMPKα expressions were tested by Western blot. The primary culture of hepatocytes was prepared and used for confirming the above signaling pathways.

Results: Obesity, IR and oxidative stress developed in HFD mice. Administration of Sc at a dose of 50mg/kg for 16 weeks effectively attenuated these changes. Further studies revealed the antagonistic effect of Sc on IR was a result of the activation of the insulin signaling pathway and AMPKα. The primary hepatocyte test, stimulated by high glucose, further confirmed that SC exerts anti-IR through the above signaling pathway and key protein.

Conclusion: These results suggested that Sc possesses not only an important novel anti-IR effect but also an anti-oxidative stress effect. These favorable effects were causally associated with weight loss and the improved glycometabolism. The underlying mechanisms might associated with the activation of the insulin signaling pathway and AMPKα. Our study promotes the understanding of the pharmacological actions of Sc, and plays a role for Sc in the effective treatment of diabetes mellitus.
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http://dx.doi.org/10.1016/j.orcp.2020.06.005DOI Listing
July 2020

Photoassisted degradation of 2,2',4,4'-tetrabrominated diphenyl ether in simulated soil washing system containing Triton X series surfactants.

Environ Pollut 2020 Oct 11;265(Pt B):115005. Epub 2020 Jun 11.

School of Environment and Energy, South China University of Technology, Guangzhou, 510006, China. Electronic address:

This study aims to use ultraviolet (UV) irradiation to decompose polybrominated diphenyl ethers (PBDEs) in the elutes and then reuse the surfactants. The results indicate that UV can remove 2,2',4,4'-tetrabrominated diphenyl ether (BDE-47) from surfactant eluents and Triton X series surfactants also can remove BDE-47 from the soil. Triton X-100 (TX-100) is the most promising surfactant during the washing and photodegradation processes. Quench experiments suggest that both O and OH• were involved in the TX-100 decomposition but only O is responsible for the degradation of BDE-47. In analysis of the photoproducts of BDE-47 by Gas Chromatography Mass Spectrum (GC-MS) and Liquid Chromatography High Resolution Mass Spectrometry (LC-HRMS), BDE-47 was mainly debrominated to the lower-brominated BDEs and then oxidized to ring-opening products. The little loss of TX-100 can mainly be attributed to the breakage of polyethylene oxide (PEO) chain. Nevertheless, the washing wastes treated by UV light can exhibit higher solubility for BDE-47 than before, indicating they can be reused for BDE-47 removal from soil. The toxicity assessment experiments were performed using Escherichia coli (E.coli) as an indicator. The results indicate that the removal of BDE-47 by UV irradiation can reduce the toxicity of eluent.
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http://dx.doi.org/10.1016/j.envpol.2020.115005DOI Listing
October 2020

A single von Willebrand factor C-domain protein acts as an extracellular pattern-recognition receptor in the river prawn .

J Biol Chem 2020 07 12;295(30):10468-10477. Epub 2020 Jun 12.

The Key Laboratory of Zoological Systematics and Application, College of Life Sciences, Hebei University, Baoding, China

The single von Willebrand factor C-domain proteins (SVWCs) are mainly found in arthropods. Their expression may be regulated by several environmental stresses, including nutritional status and bacterial and viral infections. However, the underlying regulatory mechanism is unclear. In the present study, we identified a member of the SVWC family from the river prawn as a soluble and bacteria-inducible pattern-recognition receptor (designated MnSVWC). , recombinant MnSVWC exhibited pronounced binding and Ca-dependent agglutinating abilities against diverse microbes, including Gram-negative bacteria ( and ), Gram-positive bacteria ( and ), and yeast (). ELISA assays revealed that recombinant MnSVWC recognizes a broad range of various pathogen-associated molecular patterns (PAMPs) and has high affinity to lipopolysaccharide and lysine-type and diaminopimelic acid-type peptidylglycan and d-galactose and low affinity to d-mannan and β-1,3-glucan. Mutant MnSVWC with an impaired Glu-Pro-Asn (EPN) motif displayed reduced affinity to all these PAMPs to varying extent. Moreover, MnSVWC bound to the surface of hemocytes and promoted their phagocytic activity and clearance of invasive bacteria. RNAi-mediated MnSVWC knockdown in prawn reduced the ability to clear invading bacteria, but did not block the activities of the Toll pathway or the arthropod immune deficiency (IMD) pathway, or the expression of antimicrobial peptide genes. These results indicate that MnSVWC functions as an extracellular pattern-recognition receptor in that mediates cellular immune responses by recognizing PAMPs, agglutinating invasive microbes, and promoting phagocytosis in hemocytes.
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http://dx.doi.org/10.1074/jbc.RA120.013270DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7383388PMC
July 2020

Two ferritin genes (MdFerH and MdFerL) are involved in iron homeostasis, antioxidation and immune defense in housefly Musca domestica.

J Insect Physiol 2020 07 8;124:104073. Epub 2020 Jun 8.

The Key Laboratory of Zoological Systematics and Application, College of Life Sciences, Hebei University, Baoding, Hebei 071002, China; Institute of Life Science and Green Development, Hebei University, Baoding, Hebei 071002, China. Electronic address:

Ferritin is a ubiquitous multi-subunit iron storage protein, made up of heavy chain and light chain subunits. In recent years, invertebrate ferritins have emerged as an important, yet largely underappreciated, component of host defense and antioxidant system. Here, two alternatively spliced transcripts encoding for a unique ferritin heavy chain homolog (MdFerH), and a transcript encoding for a light chain homolog (MdFerL) are cloned and characterized from Musca domestica. Comparing with MdFerH1, a fragment is absent at the 5' untranslated region of MdFerH2, where a putative iron response element is present. Amino acid sequence analysis shows that MdFerH possesses a strictly conserved ferroxidase site, while MdFerL has a putative atypical active center. Tissue distribution analysis indicates that MdFers are enriched expressed in gut. When the larvae receive diverse stimulations, including challenge by bacteria, exposure to excess Fe, doxorubicin or ultraviolet, the expression of MdFers is positively up-regulated in different degrees and different temporal patterns, indicating their potential roles in oxidative stress. The two mRNA isoforms of MdFerH appear to be differentially expressed in different tissues, but seem to show the similar expression patterns under diverse stress conditions. Further investigation reveals that silencing MdFers can alter the redox homeostasis, leading elevated mortalities of larvae following bacterial infection. Inspiringly, recombinant MdFerL produced in Pichia pastoris shows significant iron-chelating activity in vitro. These results suggest a pivotal role of ferritins from housefly in iron homeostasis, antibacterial immunity and redox balance.
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http://dx.doi.org/10.1016/j.jinsphys.2020.104073DOI Listing
July 2020

The formation pathways of polybrominated dibenzo-p-dioxins and dibenzofurans (PBDD/Fs) from pyrolysis of polybrominated diphenyl ethers (PBDEs): Effects of bromination arrangement and level.

J Hazard Mater 2020 11 28;399:123004. Epub 2020 May 28.

School of Environment and Energy, South China University of Technology, Guangzhou 510006, China; The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, Guangzhou 510006, China.

This study presents comprehensive formation pathways of polybrominated dibenzo-p-dioxins and dibenzofurans (PBDD/Fs) from the pyrolysis of polybrominated diphenyl ethers (PBDEs). A total of 23 PBDE congeners, from mono- to hepta- brominated, were selected to conduct the pyrolysis experiments. The results suggest that n-PBDEs (where n means the number of bromine substituents) can transform into n/(n-1) PBDFs and (n-1)/(n-2) PBDDs as long as they meet certain structural requirement. One single PBDE congener can only transform (if possible) specific PBDF or PBDD based on their specific brominated arrangement by direct/oxygen bridge connecting the two ortho-carbon atoms. Among all selected BDEs, we found that only 2,2',4,4',5,5'-hexaBDE (BDE-153) can transform into 2,3,7,8-tetraBDD, which is most toxic congener among these group of compounds. When the degree of bromination increased, the yield of polybromobenzene increased, while that of the PBDD/Fs decreased, suggesting that the higher PBDEs favors to break the ether bond to form polybromobenzene, while the lower PBDEs favor transformation into PBDD/Fs. We proposed that the results in this study greatly improved our understanding on the transformation of PBDD/Fs from PBDEs in the pyrolysis process.
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http://dx.doi.org/10.1016/j.jhazmat.2020.123004DOI Listing
November 2020

The Effects of Working Memory and Probability Format on Bayesian Reasoning.

Front Psychol 2020 12;11:863. Epub 2020 May 12.

School of Psychology, Central China Normal University, Wuhan, China.

Bayesian reasoning is common and critical in everyday life while the performance on Bayesian reasoning is rather poor. Previous studies showed that people could enhance their performance by applying cognitive resources under the natural frequency format condition. Working memory is one of the crucial cognitive resources in the reasoning process. However, the role of working memory on Bayesian reasoning remains unclear. In our study, we verified the effect of working memory on Bayesian reasoning by evaluating the performance of participants with high and low working memory span (WMS); we also investigated if working memory as a kind of cognitive resource can affect Bayesian reasoning performance by manipulating the cognitive load in a dual-task paradigm among participants with no-, low-, and high-loads. We found the following: (1) The Bayesian reasoning performance of high WMS participants was significantly higher than that of low WMS participants. (2) Performance under natural frequency condition was noticeably higher than that in standard probability condition. (3) Interaction between working memory and probability format was significant, and the performance of participants with high-load in natural frequency condition was higher when compared to those of participants with no- and low-load. Therefore, we can conclude that: (1) Working memory resource is a major factor in Bayesian reasoning. The performance of Bayesian reasoning is influenced by working memory span and working memory load. (2) A Bayesian facilitation effect exists, and replacing the standard probability format with a natural frequency format can significantly improve Bayesian performance. (3) Bayesian facilitation occurs only in participants with sufficient working memory resources.
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http://dx.doi.org/10.3389/fpsyg.2020.00863DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235282PMC
May 2020

Effect of nitrate on the phototreatment of Triton X-100 simulated washing waste containing 4,4'-dibromodiphenyl ether: Kinetics, products and toxicity assessment.

Sci Total Environ 2020 Aug 8;732:139247. Epub 2020 May 8.

School of Environment and Energy, South China University of Technology, Guangzhou 510006, China. Electronic address:

This study aimed to investigate the effects of nitrate on the ultraviolet (UV) treatment of simulated washing wastes containing Trion X-100 (TX-100) surfactant and 4,4'-dibromodiphenyl ether (BDE-15) pollutant. The presence of nitrate accelerated the photodegradation of BDE-15 and TX-100, because they reacted with reactive oxygen species (ROS) produced from conversion between nitrate and nitrite. Due to nitrite having a stronger radical quenching property than nitrate, nitrite hindered TX-100 decay while the photodegradation rate of BDE-15 was similar to that in the presence of nitrate. This indicated that nitrate/nitrite affected BDE-15 photodegradation by photosensitization and TX-100 loss by ROS attack. An increased TX-100 concentration increased the loss of total inorganic nitrogen possibly owing to an increase in organic nitrogen formation through TX-100 nitration reactions. At pH < 7 HOONO rapidly isomerized to NO, and at pH = 7-9 it homolyzed to ONOO, which increased OH production to decay the BDE-15 and TX-100 and also increased NO formation. BDE-15 mainly underwent debromination, and some rearrangement, ring formation, nitration and hydroxylation products were detected, indicating that the produced OH and NO attacked the BDE-15 and products. Furthermore, broken-chain, carboxylation, hydroxylation and nitro products were detected by Liquid chromatography high resolution mass spectrometry (LC-HRMS). Escherichia coli was used to assess the toxicity of washing waste containing nitrate: the presence of nitrate will increase the wastes' toxicity during UV treatment. Therefore, the presence of nitrate is deleterious to the UV treatment of washing wastes, and it is important to remove nitrates and nitrites from washing waste before UV irradiation.
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http://dx.doi.org/10.1016/j.scitotenv.2020.139247DOI Listing
August 2020

Expression and prognostic significance of pyruvate dehydrogenase kinase 1 in bladder urothelial carcinoma.

Virchows Arch 2020 Nov 9;477(5):637-649. Epub 2020 May 9.

Department of Urology, Affiliated Tumor Hospital of Nantong University, Nantong, 226361, Jiangsu, China.

Muscular infiltrating bladder urothelial carcinoma (MIBC) is a highly malignant disease with a poor prognosis. Radical cystectomy is the standard treatment. However, due to surgery and postoperative complications, the quality of life of patients is seriously affected. Therefore, it is increasingly important to find prognostic markers and new therapeutic targets for MIBC. Here, we investigated the expression of PDK1, a key regulator of glucose metabolism, in bladder urothelial carcinoma (BLCA) and its effect on prognosis. The expression pattern of PDK1 was examined by bioinformatics analysis and immunohistochemistry. A total of 101 cases of BLCA were selected for tissue microarrays (TMAs) that contained both tumour and paired normal tissues. We demonstrated that PDK1 expression was correlated with tumour grade and Ki67expression in our TMA cohort (all p values < 0.05). Kaplan-Meier survival analysis showed that patients with MIBC with high PDK1 expression had a worse prognosis than patients with low PDK1 expression (p = 0.016). Multifactor risk analysis showed that increased PDK1 expression was an independent prognostic factor affecting the overall survival of MIBC patients. GSEA showed that the mTOR pathway, HIF pathway, glycolysis, PI3K/AKT/mTOR signalling, etc. were differentially enriched in the PDK1 high expression phenotype. Hence, PDK1 may be a prognostic and therapeutic target for MIBC.
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http://dx.doi.org/10.1007/s00428-020-02782-zDOI Listing
November 2020

LncRNA AATBC regulates Pinin to promote metastasis in nasopharyngeal carcinoma.

Mol Oncol 2020 09 13;14(9):2251-2270. Epub 2020 Jun 13.

NHC Key Laboratory of Carcinogenesis and Hunan Key Laboratory of Translational Radiation Oncology, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, China.

Long noncoding RNA (lncRNA) have emerged as crucial regulators for a myriad of biological processes, and perturbations in their cellular expression levels have often been associated with cancer pathogenesis. In this study, we identified AATBC (apoptosis-associated transcript in bladder cancer, LOC284837) as a novel lncRNA. AATBC was found to be highly expressed in nasopharyngeal carcinoma (NPC), and increased AATBC expression was associated with poor survival in patients with NPC. Furthermore, AATBC promoted migration and invasion of NPC cells in vitro, as well as metastasis in vivo. AATBC upregulated the expression of the desmosome-associated protein pinin (PNN) through miR-1237-3p sponging. In turn, PNN interacted with the epithelial-mesenchymal transition (EMT) activator ZEB1 and upregulated ZEB1 expression to promote EMT in NPC cells. Collectively, our results indicate that AATBC promotes NPC progression through the miR-1237-3p-PNN-ZEB1 axis. Our findings indicate AATBC as a potential prognostic biomarker or therapeutic target in NPC.
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http://dx.doi.org/10.1002/1878-0261.12703DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7463349PMC
September 2020

Fucosidosis in a Chinese boy: a case report and literature review.

J Int Med Res 2020 Apr;48(4):300060520911269

Department of Neurology, Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian, China.

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http://dx.doi.org/10.1177/0300060520911269DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7132800PMC
April 2020

TFAM, a potential oxidative stress biomarker used for monitoring environment pollutants in Musca domestica.

Int J Biol Macromol 2020 Jul 27;155:524-534. Epub 2020 Mar 27.

Key Laboratory of zoological Systematics and Application of Hebei Province, College of Life Sciences, Hebei University, Baoding 071002, China; The International Centre for Precision Environmental Health and Governance, College of Life Sciences, Hebei University, Baoding 071002, China. Electronic address:

Mitochondrial transcription factor A (TFAM) plays a key role in regulating the transcription, replication, and maintenance of mitochondrial DNA. In the present study, a Musca domestica TFAM (MdTFAM) gene was identified and characterized. MdTFAM gene encodes 253 amino acid residues, and the protein possesses two conserved motifs of HMG (High Mobility Group) box. Expression of MdTFAM was investigated based on the qRT-PCR (quantitative real-time polymerase chain reaction) in response to three model oxidative stress-inducing agents, cadmium chloride (Cd), doxorubicin hydrochloride (DOX) and ultraviolet (UV), respectively. Results showed that Cd exposure not only generated oxidative stress and mitochondrial dysfunctions in M. domestica larvae, with a significant increase in malondialdehyde and reactive oxygen species levels, but also induced a dose-dependent increase in the expression of MdTFAM. In addition, either DOX or UV exposure also significantly up-regulated the expression of MdTFAM in M. domestica larvae. These results suggest that MdTFAM play a vital role in maintaining the redox balance and its expression may serve as a useful biomarker for monitoring the oxidative stress induced by Cd, DOX or UV.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.03.208DOI Listing
July 2020

A fibrinogen-related protein (Mnfico3) acts as a novel pattern recognition receptor in Macrobrachium nipponense.

Fish Shellfish Immunol 2020 May 3;100:272-282. Epub 2020 Mar 3.

The Key Laboratory of Zoological Systematics and Application, College of Life Sciences, Hebei University, Baoding, Hebei, 071002, China. Electronic address:

Fibrinogen-related proteins (FREPs) are widely found in both vertebrates as well as invertebrates, and they play a crucial role in host immunity. In this study, we isolated a novel ficolin gene (Mnfico3) from the oriental river prawn Macrobrachium nipponense. The complete cDNA sequence of Mnfico3 was 1133 bp long, containing an open reading frame of 765 bp coding for Mnfico3, a protein consisting of 254 amino acids. The Mnfico3 protein contained a putative N-terminal signal peptide and a fibrinogen-related protein domain present at the C-terminal. Phylogenetic analysis indicated that Mnfico3 had a closer evolutionary relationship with vertebrate ficolins than with its invertebrate homologues. Tissue distribution analysis indicated that Mnfico3 was predominantly expressed in muscle, in which its transcription was increased following bacterial challenge by Aeromonas veronii. Function analysis using recombinant protein revealed that rMnFico3 had broad-spectrum binding capacity to a variety of microorganisms and pathogen-associated molecular pattern (PAMP) ligands. Furthermore, rMnFico3 exhibited Ca-dependent agglutinating activity against microbes in vitro, and ability to attach to the hemocyte surface which promoted phagocytosis and subsequent clearance of invasive bacteria in vivo. Silencing rMnFico3 in prawn through RNAi did not alter the expression of antimicrobial peptide genes (ALF and Crustin). These results manifested that MnFico3 functioned as a potential pattern recognition receptor (PPR) to mediate cellular immune response by recognizing PAMPs, agglutinating invasive microbes, and promoting phagocytosis of hemocytes.
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http://dx.doi.org/10.1016/j.fsi.2020.02.066DOI Listing
May 2020

Peltate glandular trichomes of Colquhounia vestita harbor diterpenoid acids that contribute to plant adaptation to UV radiation and cold stresses.

Phytochemistry 2020 Apr 5;172:112285. Epub 2020 Feb 5.

State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, PR China; Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming, 650201, PR China. Electronic address:

Plant glandular trichomes (GTs) are adaptive epidermal structures that synthesize and accumulate diverse specialized metabolites well-known as defense chemicals against biotic attacks, but their roles against abiotic challenges including UV radiation and cold climates remain largely unexplored. Colquhounia vestita Wall is a Chinese-Himalayan Lamiaceae plant with dense peltate and capitate GTs on its leaf and stem surfaces under a scanning electron microscope. Three diterpenoid acids, including a clerodane 5-epi-hardwickiic acid and two labdanes polyalthic acid and E-communic acid, were identified from the peltate GTs of C. vestita through laser microdissection coupled with UPLC-MS/MS. Under UV radiation and cold stresses, the major GT component polyalthic acid increased the biomass of Arabidopsis thaliana seedlings and decreased their malondialdehyde content. Furthermore, polyalthic acid promoted photosynthetic efficiency and the expression of genes encoding peroxidative enzymes under UV radiation, and stimulated Ca elevation and the expression of calmodulin binding transcription activator gene CAMTA3 and two downstream cold-responsive genes CBF3 and RD29A under cold stress. Therefore, polyalthic acid in GTs is likely to endow the plant with enhanced tolerance to UV radiation and cold stresses, which extends the current understanding of the function of GT compounds in plant adaptation to abiotic environments.
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http://dx.doi.org/10.1016/j.phytochem.2020.112285DOI Listing
April 2020