Publications by authors named "Ting Li"

2,490 Publications

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Functional analysis of HECA variants identified in congenital heart disease in the Chinese population.

J Clin Lab Anal 2022 Aug 10:e24649. Epub 2022 Aug 10.

Children's Hospital of Fudan University, Shanghai, China.

Background: Congenital heart disease (CHD) is a class of cardiovascular defects that includes septal defects, outflow tract abnormalities, and valve defects. Human homolog of Drosophila headcase (HECA) is a novel cell cycle regulator whose role in CHD has not been elucidated. This is the first study to determine the frequency of HECA mutations in patients with CHD and the association between HECA variants and CHD.

Methods: In this study, we identified a candidate gene, HECA, by whole-exome sequencing of an atrial septal defect family. To investigate the association between HECA variants and CHD risk, targeted exon sequencing was conducted in 689 individuals with sporadic CHD. We further analyzed the effect of HECA gene abnormalities on cardiomyocyte phenotype behavior and related signaling pathways by Western blotting, reverse transcription-quantitative polymerase chain reaction, and scratch assay.

Results: We found a novel de novo mutation, c.409_410insA (p. W137fs), in the HECA gene and identified five rare deleterious variants that met the filtering criteria in 689 individuals with sporadic CHD. Fisher's exact test revealed a significant association between HECA variations and CHD compared with those in gnomADv2-East Asians(p = 0.0027). Further functional analysis suggested that the variant p. W137fs resulted in a deficiency of the normal HECA protein, and HECA deficiency altered AC16 cell cycle progression, increased cell proliferation, and migration, and promoted the activation of the PDGF-BB/PDGFRB/AKT pathway.

Conclusions: Our study identified HECA and its six rare variants, expanding the spectrum of genes associated with CHD pathogenesis in the Chinese population.
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http://dx.doi.org/10.1002/jcla.24649DOI Listing
August 2022

Retinal Microvasculature and Choriocapillaris Flow Deficit in Relation to Serum Uric Acid Using Swept-Source Optical Coherence Tomography Angiography.

Transl Vis Sci Technol 2022 Aug;11(8)

Department of Rheumatology, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.

Purpose: To explore the relationship between serum uric acid (SUA) and retinochoroidal microcirculation in the Chinese population.

Methods: This prospective cross-sectional study was conducted among the residents of Guangzhou, southern China. A commercially available optical coherence tomography angiography (OCTA) device was used to obtain the superficial vessel density (SVD) and deep vessel density in the retina and the choriocapillaris flow deficit (CFD) in the macular region. Univariable and multivariable linear regression models were used to assess the association of hyperuricemia and SUA levels with OCTA parameters.

Results: A total of 638 participants with normal SUA and 296 participants with hyperuricemia were included in the study. Parafoveal SVD was significantly reduced among the participants with hyperuricemia compared to participants with normal SUA (P < 0.001), while the parafoveal CFD was higher in hyperuricemic participants than those of normal SUA levels (P = 0.007). After adjusting for potential confounders, greater SUA levels was associated with lower SVD (β = -0.078; P < 0.001) and greater CFD (β = 0.015; P = 0.011). Gender difference analysis indicated that a 10-µmol/L increase in SUA levels among the female participants led to a 0.144 decrease in SVD (P < 0.001), but it was not statistically significant for the male participants (P = 0.653).

Conclusions: An elevated uric acid level and its fluctuations were independently associated with impaired retinal and choroidal microcirculation using OCTA in the study population. Women appear to be more sensitive to high SUA levels than men.

Translational Relevance: Elevating uric acid concentration may play a role in the development and progression of cardiovascular diseases through microvascular alteration, as demonstrated by OCTA parameters.
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http://dx.doi.org/10.1167/tvst.11.8.9DOI Listing
August 2022

Fear of cancer recurrence and hope level in patients receiving surgery for non-small cell lung cancer: a study on the mediating role of social support.

Support Care Cancer 2022 Aug 10. Epub 2022 Aug 10.

Department of Thoracic Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China.

Purpose: To study the level of fear of cancer recurrence (FCR) in patients receiving surgery for non-small cell lung cancer (NSCLC) and explore related factors that can increase levels of hope in this population, enhance the confidence to defeat the disease, and thus increase the quality of life.

Methods: A total of 327 postoperative NSCLC patients from the National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College were enrolled. All participants completed the General Questionnaire, Fear of Progression Questionnaire-Short Form (FoP-Q-SF), Herth Hope Index (HHI) Scale, and Social Support Rating Scale (SSRS).

Results: The mean FoP-Q-SF score was (30.3 ± 9.48) points in postoperative NSCLC patients, Among them, there were 188 patients (57.5%) who had psychological dysfunction, indicated by a score of > / = 34. Patient sex and family income were independent risk factors for FCR (both p < 0.05). Correlation analysis revealed a negative association between FCR and hope level (p < 0.05) and a positive association between hope level and social support (p < 0.05). Notably, social support mediated the association between FCR and hope in patients receiving surgery for NSCLC (contribution effect: 30.24%).

Conclusion: Postoperative NSCLC patients experience a moderate level of FCR, especially females and those with a low family income. Social support partially mediates the relationship between FCR and hope in this population. Therefore, an increase in the level of social support can increase hope among these patients and advancing recovery.
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http://dx.doi.org/10.1007/s00520-022-07318-6DOI Listing
August 2022

N6-methyladenosine-mediated CELF2 regulates CD44 alternative splicing affecting tumorigenesis via ERAD pathway in pancreatic cancer.

Cell Biosci 2022 Aug 8;12(1):125. Epub 2022 Aug 8.

Department of Hepatopancreatobiliary Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, 350001, Fujian, China.

Background: Alternative splicing (AS) of genes has been found to affect gene stability, and its abnormal regulation can lead to tumorigenesis. CELF2 is a vital splicing factor to participate in mRNA alternative splicing. Its downregulation has been confirmed to promote the occurrence and development of pancreatic cancer (PC). However, the regulatory role and mechanisms in PC has not been elucidated.

Results: CELF2 was downregulated in PC tissues, which affected tumor TNM stage and tumor size, and low expression of CELF2 indicated a poor prognosis of PC. In vivo and in vitro experiments showed that abnormal expression of CELF2 affected the stemness, apoptosis, and proliferation of PC cells. Furthmore, we also found that CELF2 was targeted by ALKBH5 for m6A modification, leading to CELF2 degradation by YTHDF2. Bioinformatic analysis of AS model based on the TCGA database indicated that CELF2 could target CD44 to form different spliceosomes, thereby affecting the biological behavior of PC cells. The conversion of CD44s to CD44V is the key to tumorigenesis. Transcriptomic analysis was conducted to reveal the mechanism of CELF2-mediated CD44 AS in PC. We found that CELF2-mediated splicing of CD44 led to changes in the level of endoplasmic reticulum stress, further regulating the endoplasmic reticulum-associated degradation (ERAD) signaling pathway, thereby affecting apoptosis and cell stemness. In addition, ERAD signaling pathway inhibitor, EerI, could effectively reverse the effect of CD44 on tumors.

Conclusions: This study indicates that N6-methyladenosine-mediated CELF2 promotes AS of CD44, affecting the ERAD pathway and regulating the biological behavior of PC cells. CELF2 is expected to be a new target for targeted-drug development.
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http://dx.doi.org/10.1186/s13578-022-00844-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9361702PMC
August 2022

Biocompatible Ionic Liquids in High-Performing Organic Electrochemical Transistors for Ion Detection and Electrophysiological Monitoring.

ACS Nano 2022 Aug 8. Epub 2022 Aug 8.

School of Electrical and Electronic Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798.

Organic electrochemical transistors (OECTs) have recently attracted attention due to their high transconductance and low operating voltage, which makes them ideal for a wide range of biosensing applications. Poly-3,4-ethylenedioxythiophene:poly-4-styrenesulfonate (PEDOT:PSS) is a typical material used as the active channel layer in OECTs. Pristine PEDOT:PSS has poor electrical conductivity, and additives are typically introduced to improve its conductivity and OECT performance. However, these additives are mostly either toxic or not proven to be biocompatible. Herein, a biocompatible ionic liquid [MTEOA][MeOSO] is demonstrated to be an effective additive to enhance the performance of PEDOT:PSS-based OECTs. The influence of [MTEOA][MeOSO] on the conductivity, morphology, and redox process of PEDOT:PSS is investigated. The PEDOT:PSS/[MTEOA][MeOSO]-based OECT exhibits high transconductance (22.3 ± 4.5 mS μm), high μC* (the product of mobility μ and volumetric capacitance C*) (283.80 ± 29.66 F cm V s), fast response time (∼40.57 μs), and excellent switching cyclical stability. Next, the integration of sodium (Na) and potassium (K) ion-selective membranes with the OECTs is demonstrated, enabling selective ion detection in the physiological range. In addition, flexible OECTs are designed for electrocardiography (ECG) signal acquisition. These OECTs have shown robust performance against physical deformation and successfully recorded high-quality ECG signals.
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http://dx.doi.org/10.1021/acsnano.2c02191DOI Listing
August 2022

3D-printed high-density polyethylene scaffolds with bioactive and antibacterial layer-by-layer modification for auricle reconstruction.

Mater Today Bio 2022 Dec 15;16:100361. Epub 2022 Jul 15.

Guangdong Engineering Research Center for Translation of Medical 3D Printing Application, Guangdong Provincial Key Laboratory of Medical Biomechanics, National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, China.

High-density polyethylene (HDPE) is a promising material for the development of scaffold implants for auricle reconstruction. However, preparing a personalized HDPE auricle implant with favorable bioactive and antibacterial functions to promote skin tissue ingrowth is challenging. Herein, we present 3D-printed HDPE auricle scaffolds with satisfactory pore size and connectivity. The layer-by-layer (LBL) approach was applied to achieve the improved bioactive and antibacterial properties of these 3D printed scaffolds. The HDPE auricle scaffolds were fabricated using an extrusion 3D printing approach, and the individualized macrostructure and porous microstructure were both adjusted by the 3D printing parameters. The polydopamine (pDA) coating method was used to construct a multilayer ε-polylysine (EPL) and fibrin (FIB) modification on the surface of the 3D HDPE scaffold the LBL self-assembly approach, which provides the bioactive and antibacterial properties. The results of the experiments using an animal model showed that LBL-coated HDPE auricular scaffolds were able to significantly enhance skin tissue ingrowth and ameliorate the inflammatory response caused by local stress. The results of this study suggest that the combination of the 3D printing technique and surface modification provides a promising strategy for developing personalized implants with biofunctional coatings, which show great potential as a scaffold implant for auricle reconstruction applications.
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http://dx.doi.org/10.1016/j.mtbio.2022.100361DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9352972PMC
December 2022

How to Achieve Sufficient Endogenous Insulin Suppression in Euglycemic Clamps Assessing the Pharmacokinetics and Pharmacodynamics of Long-Acting Insulin Preparations Employing Healthy Volunteers.

Front Pharmacol 2022 22;13:899798. Epub 2022 Jul 22.

Department of Endocrinology and Metabolism, Sichuan University West China Hospital, Chengdu, China.

The therapeutic effect of basal insulin analogs will be sustained at a rather low insulin level. When employing healthy volunteers to assess the pharmacokinetics (PK) and pharmacodynamics (PD) of long-acting insulin preparations by euglycemic clamp techniques, endogenous insulin cannot be ignored and sufficient endogenous insulin inhibition is crucial for the PD and/or PK assessment. This study aimed to explore a way to sufficiently inhibit endogenous insulin secretion. Healthy Chinese male and female volunteers were enrolled. After a subcutaneous injection of insulin glargine (IGlar) (LY2963016 or Lantus) (0.5 IU/kg), they underwent a manual euglycemic clamp for up to 24 h where the target blood glucose (BG) was set as 0.28 mmol/L below the individual's baseline. Blood samples were collected for analysis of PK/PD and C-peptide. The subjects fell into two groups according to the reduction extent of postdose C-peptide from baseline. After matching for the dosage proportion of Lantus, there were 52 subjects in group A (C-peptide reduction<50%) and 26 in group B (C-peptide reduction≥50%), respectively. No significant difference was detected in age, body mass index, the proportion of Latus treatment and female participants. A lower basal BG was observed in group B compared to group A (4.35 ± 0.26 vs 4.59 ± 0.22 mmol/L, < 0.05). The clamp studies were all conducted with high quality (where BG was consistently maintained around the target and exhibited a low variety). The binary logistic regression analysis indicated low basal BG as an independent factor for the success of sufficient endogenous insulin suppression. In conclusion, setting a lower sub-baseline target BG (e.g., 10% instead of 5% below baseline) might be an approach to help achieve sufficient endogenous insulin suppression in euglycemic clamps with higher basal BG levels (e.g., beyond 4.60 mmol/L).
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http://dx.doi.org/10.3389/fphar.2022.899798DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354408PMC
July 2022

Controlling sustained statins release in multi-layered composite scaffolds for healing of osteoporotic bone defects.

Biomater Adv 2022 Jun 5;137:212838. Epub 2022 May 5.

Department of Orthopaedic Surgery, Sichuan Provincial People's Hospital, Sichuan Academy of Medical Science, Affiliated Hospital of University of Electronic Science and Technology, Chengdu 610072, China. Electronic address:

The risk of fragility fracture sharply increases due to the decreased bone mineral density and toughness in patients with osteoporosis (OP). The local use of bone tissue scaffolds with both mechanical stability and drug-delivery functionality is one of the key strategies for the efficient curing of OP. In this work, we reported a layer-by-layer constructing strategy to fabricate 3-D composite bone tissue scaffolds (eSTPS) by assembling β-tri‑calcium phosphate (β-TCP)/polycaprolactone (PCL) microchips and lovastatin-loaded nanofiber membranes (eLOV/PCL). The eSTPS scaffolds show a strong and suited compressive strength as well as long-term delivery of lovastatin. The in vitro tests indicate well biocompatibility and alkaline phosphatase activity of the scaffolds. The eSTPS scaffolds were implanted into the femur of OP modeled rabbits. After 12 weeks curing, the bone parameters are significantly improved, meanwhile ingrowth of new bone and vascular-like tissue were observed. These results suggest the eSTPS scaffolds to be a promising candidate for the local treatment of OP.
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http://dx.doi.org/10.1016/j.bioadv.2022.212838DOI Listing
June 2022

FOXF2 Regulates PRUNE2 Transcription in the Pathogenesis of Colorectal Cancer.

Technol Cancer Res Treat 2022 Jan-Dec;21:15330338221118717

Department of Gastroenterology, The First People's Hospital of Yunnan Province, Kunming, Yunnan, China.

Forkhead box F2, a member of the Forkhead box transcription factor superfamily, plays an important role in several types of cancer. However, the mechanisms of Forkhead box F2 in the progression of colorectal cancer remain unclear. PRUNE2 is closely associated with prostate cancer, neuroblastoma, glioblastoma, and melanoma. The relationship between Forkhead box F2 and PRUNE2 in colorectal cancer remains unknown. We investigated the effects of Forkhead box F2 upregulation on colorectal cancer cell behavior using Cell Counting Kit-8, colony formation, flow cytometry, Transwell, reverse transcription quantitative polymerase chain reaction and Western blot analyses. Nude mouse xenografts were established to investigate the effect of Forkhead box F2 upregulation on the growth of colorectal cancer cells. Dual-luciferase reporter assays were performed to confirm the Forkhead box F2 regulation of PRUNE2 transcription. A series of assays was performed in cells with Forkhead box F2 upregulation and PRUNE2 knockdown to elucidate the function and regulatory effects of Forkhead box F2 on PRUNE2 transcription in colorectal cancer. Forkhead box F2 was downregulated in colorectal cancer tissues compared with adjacent tissues. Forkhead box F2 overexpression significantly suppressed the proliferation and invasion of colorectal cancer cells and . Moreover, Forkhead box F2 directly targeted PRUNE2 to promote its transcription in colorectal cancer cells. Furthermore, PRUNE2 mediated the Forkhead box F2-regulated proliferation and invasion of colorectal cancer cells. Additionally, we demonstrated a significant positive correlation between Forkhead box F2 and PRUNE2 mRNA levels in colorectal cancer tissues. These results indicated that Forkhead box F2 and PRUNE2 in combination may serve as a prognostic biomarker for colorectal cancer and that Forkhead box F2 upregulation inhibits the proliferation and invasion of colorectal cancer cells by upregulating PRUNE2.
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http://dx.doi.org/10.1177/15330338221118717DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9358570PMC
August 2022

Kangfuxiaoyanshuan alleviates uterine inflammation and adhesion via inhibiting NF-κB p65 and TGF-β/MMP-2 signaling pathway in pelvic inflammatory disease rats.

Front Pharmacol 2022 18;13:894149. Epub 2022 Jul 18.

Department of Microecological Laboratory, Beijing Obstetrics and Gynecology Hospital, Capital Medical University. Beijing Maternal and Child Health Care Hospital, Beijing, China.

Pelvic inflammatory disease (PID) is infection-induced inflammation of the female upper reproductive tract that results in high fever, ectopic pregnancy, infertility, and varying degrees of chronic pelvic pain. Recent clinical studies have shown that Kangfuxiaoyanshuan (KFXYS), a Traditional Chinese Medicine (TCM) formulation, may short the course of the disease and reduce the occurrence of PID sequelae, but its pharmacological action and potential mechanism have not been fully elucidated. Here, we aimed to investigate the therapeutic effects and mechanism of KFXYS in rats with PID. A PID rat model was constructed through endometrial mechanical injury and pathogen infection. The rectal temperature was measured during the 14-days course of treatment, and the white blood cell (WBC) count in the blood and the levels of cytokines (IFN-γ, IL-1β, IL-4, TNF-α) in the serum were evaluated by ELISA. Hematoxylin and eosin (HE) staining was performed to analyze pathological changes, and transmission electron microscopy (TEM) was used to observe ultrastructural changes. The p-p65/p65 protein expression was evaluated by western blotting and the levels of MMP-2 and TGF-β in adhesion tissues were assessed by immunohistochemistry. KFXYS lowered the rectal temperature and the WBC counts in the blood in the acute stage of PID and alleviated inflammatory cell infiltration of the uterus, especially when combined with levofloxacin. KFXYS significantly decreased the levels of proinflammatory cytokines (IFN-γ, IL-1β, IL-4) and adhesion-related factors (TNF-α) and protected the ultrastructure of endometrial epithelial cells. Mechanistically, KFXYS inhibited the NF-κB activation by decreasing phosphorylation of p65, thus the alleviation of inflammation further reduced the expression of TGF-β and MMP-2, and inhibited the occurrence of uterine adhesions. These results revealed that KFXYS alleviated pelvic inflammation and effectively inhibits inflammation-associated adhesion, which indicated the potential role of KFXYS for treatment of PID and the prevention of PID sequelae.
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http://dx.doi.org/10.3389/fphar.2022.894149DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340273PMC
July 2022

Profiling of proteome changes in plasma of HIV-infected patients receiving antiretroviral therapy.

Proteomics Clin Appl 2022 Aug 3:e2100099. Epub 2022 Aug 3.

School of Life Sciences, Tsinghua University, Beijing, 100084, China.

Purpose: Antiretroviral therapy (ART) prevents HIV-1 onward transmission and disease progression, leading to excellent prognosis in people living with HIV-1 (PWH). However, side effects, complications and impaired immune reconstitution persist in some patients treated with ART. We aimed to profile proteome changes in plasma before and after ART to identify the molecular pathways altered by ART.

Experimental Design: Quantitative proteomics analysis based on tandem mass tag labeling was used to profile proteome changes of paired plasma samples from HIV-1 patients before receiving ART and after ART treatment.

Results: A total of 1398 protein groups (PGs) were identified, in which 18 proteins were downregulated and 50 were upregulated in plasma from ART treated patients. Based on Ingenuity Pathway analysis, gap junction signaling and actin cytoskeleton signaling were enriched among upregulated proteins, while downregulated proteins were mainly participated in IL-15 signaling pathway. Patients with the low level of CSF1R and the high levels of MINPP1 and TGM3 showed better CD4 T-cell recovery.

Conclusions: The present study provided plasma proteome changes after ART to elucidate the underlying mechanistic pathways in response to ART, and also identified potential targets to prompt immune reconstitution. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/prca.202100099DOI Listing
August 2022

Operating interfaces to synthesize [email protected] nanoparticles by modifying the heating process.

Nanoscale 2022 Aug 2. Epub 2022 Aug 2.

Key Laboratory of Anisotropy and Texture of Materials (Ministry of Education), School of Materials Science and Engineering, Northeastern University, Shenyang 110819, China.

A facile strategy to operate interfaces when synthesizing [email protected] nanoparticles (NPs) has been proposed. Two interfaces are indispensable to obtain the high ordering L1-FePt structure. One is the mismatched interfaces between the initial γ-PtBi nuclei and the disordered fcc-FePt phase. The other is the grown coherent interfaces between the L1-FePt and Bi-rich phases. Increasing the heating rates when the temperature rises from 120 °C to 310 °C benefits the formation of mismatched interfaces and improves the uniformity of phases and composition in NPs. Reducing the heating rate at higher temperature ensures sufficient time for Bi to diffuse across the coherent interface, which facilitates the disorder-order transition of L1-FePt NPs. This study provides a new perspective on operating interfaces during the wet-chemical synthesis process.
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http://dx.doi.org/10.1039/d2nr01493aDOI Listing
August 2022

Single-step etched two-dimensional polarization splitting dual-band grating coupler for wavelength (de)multiplexing.

Opt Lett 2022 Aug;47(15):3924-3927

Diffractive periodic-structure-based grating couplers (GCs) are the most widely used devices for light coupling between optical fibers and integrated photonic devices. However, conventional GCs have limited wavelength operation and are polarization specific, which is due to the intrinsic radiation angle dependency on both wavelength and polarization. Here we propose and experimentally demonstrate a polarization-splitting dual-band grating coupler (PS-DBGC) for polarization diversity and wavelength division (de)multiplexing (WDM) operation. The four-port two-dimensional PS-DBGC is based on a periodically arranged structure with square holes, and requires only a single etch step in a 340-nm silicon-on-insulator platform. The simulation predicts that the maximum coupling efficiency (CE) of the proposed PS-DBGC is -2.8 dB and -4.6 dB for the O- and C-band, respectively. The measured peak CEs of the fabricated device are -4.7 dB at 1280 nm and -8.4 dB at 1522 nm. We anticipate that this PS-DBGC could potentially improve the performance of any future integrated WDM passive optical network.
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http://dx.doi.org/10.1364/OL.462937DOI Listing
August 2022

Effects of indoor VOCs from paint on human brain activities during working memory tasks: An electroencephalogram study.

Indoor Air 2022 Jul;32(7):e13062

Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, China.

As more and more people stay inside the building for a long time, the indoor environment has a great effect on their health, mood, and work efficiency. Electroencephalography (EEG) signals reflect electrical activity originating from neuronal firing when a task or activity is performed. Since there was no study on the effect of indoor air on nerves, this study utilized EEG to preliminarily explore the brain functions of subjects during working memory tasks with different difficulties. The subjects were divided into two groups according to the volatile organic compounds (VOCs) as odor irritants in the air. We expected to find the difference in subjects' EEG signals between VOCs and low-VOCs. The EEG signals from 60 electrodes were analyzed by event-related potential (ERP), event-related spectral power (ERSP), and correlation network methods to describe the brain activity. We compared the results of subjects in VOCs and low-VOCs and found significant differences between ERP and ERSP in the alpha band during a simple working memory task. Subjects were more sensitive to the VOCs in simple tasks than in complex tasks. Our work provided evidence of odor effects on brain functions and could be used to guide the design of indoor odor in home, offices, and classrooms.
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http://dx.doi.org/10.1111/ina.13062DOI Listing
July 2022

Protective effects and mechanisms of melatonin on stress myocardial injury in rats.

J Cardiovasc Pharmacol 2022 Jun 4. Epub 2022 Jun 4.

Pharmacology Laboratory, School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.

Abstract: Prolonged and intense stress can exceed the body's normal self-regulation and limited compensatory and repair capacity, resulting in pathological damage to the body. In this study, we established a rat stress myocardial injury model to explore the protective effect of melatonin (MLT) on stress myocardial injury and its possible mechanisms of action. Adult female Sprague-Dawley (SD) rats were randomly divided into 5 groups: blank control group (NC), stress myocardial injury group (SMI), MLT low-dose group (MLTL), MLT medium-dose group (MLTM), and MLT high-dose group (MLTH), and 10 rats in each group were used to establish a stress myocardial injury model by the water immersion restraint method. We observed the changes in body weight and tail vein glucose of each group. Serum levels of corticosterone (Cort), creatine kinase isoenzyme (CK-MB), Troponin Ⅰ (Tn-Ⅰ) and activity of lactic acid dehydrogenase (LDH) were measured by ELISA. Transcriptome sequencing was used to find differentially expressed genes in the control and model groups and the results were verified by fluorescence quantitative RT-qPCR. HE staining was used to visualize the pathological changes in the heart tissue of each group and Western Blot was used to study the differences in protein expression in the cardiomyocytes of each group to further corroborate the results. The body weight growth rate of rats in the SMI group was significantly lower than that of the NC group (P < 0.01), and the body weight growth rate of rats in the MLTH group was significantly higher than that of the SMI group (P < 0.05) with no significant difference compared to the NC group rats. The mean blood glucose of rats in the SMI group was significantly higher compared to the NC group (P < 0.001), while the mean blood glucose of rats in the MLT administration groups was dose-dependently reduced compared to the SMI group. By RNA-seq and bioinformatics tools such as KEEG and GO, we found that the circadian clock-related genes Ciart, Arnt1, Per1, and Dbp were significantly down-regulated in the SMI group during water immersion stress, and differentially expressed genes were enriched in the p38MAPK signaling pathway and p53 signaling pathway. Moreover, genes related to inflammation and apoptosis were differentially expressed. ELISA results showed that Cort, CK-MB and Tn-Ⅰ levels were significantly higher in the SMI group compared to the NC group (p < 0.01) and that melatonin reduced the levels of Cort, CK-MB, and Tn-Ⅰ and decreased LDH activity in rat serum. HE staining results showed that melatonin could attenuate stress-generated myocardial injury. Western Blot showed that melatonin reduced the expression of p38MAPK, p53, Bax, and Caspase3 and increased the expression of Bcl-2 protein in rat heart. Melatonin can inhibit myocardial injury caused by water immersion, and its mechanism of action may be related to the regulation of the expression of circadian clock genes such as Ciart, Arnt1, Per1, and Dbp, the inhibition of the expression of pro-apoptotic proteins such as p38MAPK, p53, Bax and Caspase3, and the increase of the expression of Bcl-2 anti-apoptotic protein.
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http://dx.doi.org/10.1097/FJC.0000000000001312DOI Listing
June 2022

Adsorption of Phthalate Acid Esters by Activated Carbon: The Overlooked Role of the Ethanol Content.

Foods 2022 Jul 15;11(14). Epub 2022 Jul 15.

State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, Beijing 100029, China.

Ethanol has great effects on the adsorption of phthalate acid esters (PAEs) on activated carbon (AC), which are usually overlooked and hardly studied. This study investigated the overlooked effects of ethanol on the adsorption of PAEs in alcoholic solutions. The adsorption capacities of dibutyl phthalate (DBP) on AC in solutions with ethanol contents of 30, 50, 70, and 100 v% were only 59%, 43%, 19%, and 10% of that (16.39 mg/g) in water, respectively. The ethanol content increase from 50 v% to 100 v% worsened the adsorption performances significantly with the formation of water-ethanol-DBP clusters (decreasing from 13.99 mg/g to 2.34 mg/g). The molecular dynamics simulation showed that the DBP tended to be distributed farther away from the AC when the ethanol content increased from 0 v% to 100 v% (the average distribution distance increased from 5.25 Å to 15.3 Å). The PAEs with shorter chains were more affected by the presence of ethanol than those with longer chains. Taking DBP as an example, the adsorption capacity of AC in ethanol (0.41 mg/g) is only 2.2% of that in water (18.21 mg/g). The application results in actual Baijiu samples showed that the adsorption of PAEs on AC had important effects on the Baijiu flavors.
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http://dx.doi.org/10.3390/foods11142114DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323295PMC
July 2022

The Mechanism and Role of ADAMTS Protein Family in Osteoarthritis.

Biomolecules 2022 07 8;12(7). Epub 2022 Jul 8.

Department of Orthopedics, Second Affiliated Hospital of Nanchang University, 1 Minde Road, Nanchang 330000, China.

Osteoarthritis (OA) is a principal cause of aches and disability worldwide. It is characterized by the inflammation of the bone leading to degeneration and loss of cartilage function. Factors, including diet, age, and obesity, impact and/or lead to osteoarthritis. In the past few years, OA has received considerable scholarly attention owing to its increasing prevalence, resulting in a cumbersome burden. At present, most of the interventions only relieve short-term symptoms, and some treatments and drugs can aggravate the disease in the long run. There is a pressing need to address the safety problems due to osteoarthritis. A disintegrin-like and metalloprotease domain with thrombospondin type 1 repeats (ADAMTS) metalloproteinase is a kind of secretory zinc endopeptidase, comprising 19 kinds of zinc endopeptidases. ADAMTS has been implicated in several human diseases, including OA. For example, aggrecanases, ADAMTS-4 and ADAMTS-5, participate in the cleavage of aggrecan in the extracellular matrix (ECM); ADAMTS-7 and ADAMTS-12 participate in the fission of Cartilage Oligomeric Matrix Protein (COMP) into COMP lyase, and ADAMTS-2, ADAMTS-3, and ADAMTS-14 promote the formation of collagen fibers. In this article, we principally review the role of ADAMTS metalloproteinases in osteoarthritis. From three different dimensions, we explain how ADAMTS participates in all the following aspects of osteoarthritis: ECM, cartilage degeneration, and synovial inflammation. Thus, ADAMTS may be a potential therapeutic target in osteoarthritis, and this article may render a theoretical basis for the study of new therapeutic methods for osteoarthritis.
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http://dx.doi.org/10.3390/biom12070959DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313267PMC
July 2022

Comparing Bayesian-Based Reconstruction Strategies in Topology-Based Pathway Enrichment Analysis.

Biomolecules 2022 06 28;12(7). Epub 2022 Jun 28.

Wisdom Lake Academy of Pharmacy, Xi'an Jiaotong-Liverpool University, Suzhou 215123, China.

The development of high-throughput omics technologies has enabled the quantification of vast amounts of genes and gene products in the whole genome. Pathway enrichment analysis (PEA) provides an intuitive solution for extracting biological insights from massive amounts of data. Topology-based pathway analysis (TPA) represents the latest generation of PEA methods, which exploit pathway topology in addition to lists of differentially expressed genes and their expression profiles. A subset of these TPA methods, such as BPA, BNrich, and PROPS, reconstruct pathway structures by training Bayesian networks (BNs) from canonical biological pathways, providing superior representations that explain causal relationships between genes. However, these methods have never been compared for their differences in the PEA and their different topology reconstruction strategies. In this study, we aim to compare the BN reconstruction strategies of the BPA, BNrich, PROPS, Clipper, and Ensemble methods and their PEA and performance on tumor and non-tumor classification based on gene expression data. Our results indicate that they performed equally well in distinguishing tumor and non-tumor samples (AUC > 0.95) yet with a varying ranking of pathways, which can be attributed to the different BN structures resulting from the different cyclic structure removal strategies. This can be clearly seen from the reconstructed JAK-STAT networks by different strategies. In a nutshell, BNrich, which relies on expert intervention to remove loops and cyclic structures, produces BNs that best fit the biological facts. The plausibility of the Clipper strategy can also be partially explained by intuitive biological rules and theorems. Our results may offer an informed reference for the proper method for a given data analysis task.
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http://dx.doi.org/10.3390/biom12070906DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313337PMC
June 2022

Effect of Dietary Supplementation with Calcium, Phosphorus and Vitamin D on Growth Performance, Nutrient Digestibility, and Serum Biochemical Parameters of Growing Blue Foxes.

Animals (Basel) 2022 Jul 15;12(14). Epub 2022 Jul 15.

College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China.

Based on the randomized design, a 3 × 3 factorial experiment was designed to examine the effects of dietary calcium (Ca), phosphorus (P), and vitamin D (VD) supplemental levels with a fixed 1.5/1 ratio of Ca to P on the growth performance, nutrient digestibility, and serum biochemical indices blue foxes' growth. In total, 135 male blue foxes with the age of 60 days were randomly divided into 9 groups each with 15 blue foxes. The blue foxes belonging to the nine treatment groups were fed Ca supplementation (0%, 0.4%, or 0.8%) and VD supplementation (1000, 2000, or 4000 IU/kg DM). The base diet contained 0.8% Ca and 327 IU/kg VD. The dosage of VD in blue foxes showed a significant impact on their growth performance ( < 0.05). The Ca dosage had a linear effect on the digestibility of the CP and carbohydrates (CHO) ( < 0.05). In conclusion, the results indicated that the Ca and VD doses showed promising effects on growth performance and nutrient digestibility in growing blue foxes and could reduce fecal N and P via improvement in protein and P utilization.
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http://dx.doi.org/10.3390/ani12141814DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9311545PMC
July 2022

Integrated bioinformatic analysis of dysregulated microRNA-mRNA co-expression network in ovarian endometriosis.

Acta Obstet Gynecol Scand 2022 Jul 25. Epub 2022 Jul 25.

Department of Gynecology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University. Beijing Maternal and Child Health Care Hospital, Beijing, China.

Introduction: Ovarian endometriosis is a frequently occurring gynecological disease with large socioeconomic impact. Accumulating evidence has suggested that aberrant miRNA-mRNA interactions are involved in the pathogenesis and progression of ovarian endometriosis. This study aims to identify key miRNAs in ovarian endometriosis by using integrated bioinformatic analysis of a dysregulated miRNA-mRNA co-expression network.

Material And Methods: Expression profiling of miRNA and mRNA in three normal endometria and five pairs of ectopic/eutopic endometria from patients with ovarian endometriosis was determined by high-throughput sequencing techniques. The data were then integrated with the public sequencing datasets (GSE105764 and GSE105765) using a non-biased approach and a miRNA-mRNA co-expression regulatory network was constructed by in-depth bioinformatic analysis.

Results: The constructed miRNA-mRNA network included 87 functionally DEMs, 482 target mRNAs and 1850 paired miRNA-mRNA regulatory interactions. Specifically, five miRNAs (miR-141-3p, miR-363-3p, miR-577, miR-767-5p, miR-96-5p) were gradually decreased and two miRNAs (miR-493-5p, miR-592) were gradually increased from normal endometria to eutopic endometria, and then ectopic endometria tissues. Importantly, miR-141-3p, miR-363-3p and miR-96-5p belonged to the miR-200 family, miR-106a-363 cluster and miR-183/96/182 cluster, respectively. Their target mRNAs were mainly associated with cell adhesion, locomotion and binding, which are suggested to play vital regulatory roles in the pathogenesis of ovarian endometriosis.

Conclusions: Integrated bioinformatic analysis of the miRNA-mRNA co-expression network defines the crucial roles of the miR-200 family, miR-106a-363 cluster and miR-183/96/182 cluster in the pathogenesis of ovarian endometriosis. Further in-depth functional studies are needed to unveil the molecular mechanisms of these miRNAs, and may provide clues for the optimization of therapeutic strategies for ovarian endometriosis.
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http://dx.doi.org/10.1111/aogs.14430DOI Listing
July 2022

Association of Mu-Opioid Receptor Expression With Long-Term Survival and Perineural Nerve Invasion in Patients Undergoing Surgery for Ovarian Cancer.

Front Oncol 2022 7;12:927262. Epub 2022 Jul 7.

Department of Anesthesiology, Zhongshan Hospital, Fudan University, Shanghai, China.

Background: Opioids are widely used during primary debulking surgery (PDS) for ovarian cancers, and a high mu-opioid receptor (MOR) expression predicts worse cancer outcomes. However, the impact of MOR expression on survival outcomes in ovarian cancers is still not clear.

Methods: A retrospective cohort study was conducted in patients who underwent PDS in ovarian cancer patients. MOR expression was measured in tumor and normal tissue. Primary outcomes were overall survival (OS) and disease-free survival (DFS). Secondary outcomes included perineural invasion (PNI), intraoperative sufentanil consumption, length of stay (LOS), and verbal numerical rating scale (VNRS) on postoperative day 1 (POD1), POD3, and POD5.

Results: After propensity score matching, a total of 366 patients were finally enrolled in this study. There were no significant differences in OS rates in patients with high versus low levels of MOR (1-year OS: 82.9% versus 83.3%, 3-year: 57.8% versus 59.1%, 5-year: 22.4% versus 23.1%,respectively) in the ovarian cancers. There were no significant differences in DFS between the groups. Intraoperative sufentanil consumption was higher in the MOR high-expression group compared with the MOR low-expression group. Tumors expressing high levels of MOR showed higher rates of PNI. VNRS in the MOR high-expression group was higher on POD1.

Conclusion: MOR is not an independent predictor of worse survival in ovarian cancers but is associated with high rates of perineural invasion.
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http://dx.doi.org/10.3389/fonc.2022.927262DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302566PMC
July 2022

Pan-Soft Tissue Sarcoma Analysis of the Incidence, Survival, and Metastasis: A Population-Based Study Focusing on Distant Metastasis and Lymph Node Metastasis.

Front Oncol 2022 7;12:890040. Epub 2022 Jul 7.

Department of Bone and Soft Tissue Tumor, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China.

Background: The rarity and complexity of soft tissue sarcoma (STS) make it a challenge to determine the incidence, survival, and metastasis rates. In addition, the clinicopathological risk factors for lymph node metastasis have rarely been reported.

Methods: Data on patients diagnosed with STS in the SEER database from 2000 to 2018 were extracted by SEER*Stat 8.3.9.1, and the incidence trend was calculated by Joinpoint 4.9 software. The KM method was used to calculate the survival curve, and the log-rank method was used to compare differences in the survival curves. The clinicopathological risk factors for lymph node metastasis were screened by logistic regression.

Results: Among the 35987 patients, 4299 patients (11.9%) had distant metastasis. The overall lymph node metastasis rate was 6.02%, which included patients suffering from both lymph node and distant metastasis. Considering that some lymph node metastases might be accompanying events of distant metastasis, the rate of only lymph node metastasis in STS patients decreased to 3.42% after excluding patients with distant metastasis. Patients with only lymph node metastases (N1/2M0) had a significantly worse prognosis than those without metastases (N0M0) but a better prognosis than those with only distant metastases (N0M1) (p<0.0001). In the multivariate logistic analysis, STS patients with larger tumors located in the head and neck, viscera, retroperitoneum, and certain specific pathological subtypes (compared with the liposarcoma), such as undifferentiated pleomorphic sarcoma, rhabdomyosarcoma, endometrial stromal sarcoma, gastrointestinal stromal tumor, synovial sarcoma, and angiosarcoma, had a higher risk of lymph node metastasis.

Conclusions: Lymph node metastasis is rare in STS, and the metastasis rate is significantly different among the different pathological types. Tumor size, location, and pathological subtype are significantly associated with the risk of lymph node metastasis. The overall survival of patients with lymph node metastasis is better than that of patients with distant metastasis, which suggests a more precise prognosis evaluation should be performed in these AJCC stage IV STS patients.
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http://dx.doi.org/10.3389/fonc.2022.890040DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9303001PMC
July 2022

Tricyclic Aza-Andrographolide Derivatives from Late-Stage Hydroamination and Their Anti-human Coronavirus (Anti-HCoV) Activity.

ACS Omega 2022 Jul 7;7(28):24824-24837. Epub 2022 Jul 7.

BayRay Innovative Center, Shenzhen Bay Laboratory, Shenzhen 518032, China.

A late-stage functionalization (LSF) of the natural product andrographolide for the efficient assembly of a range of structurally interesting and diverse tricyclic-aza derivatives was developed. The key to the diversification is a photo-catalyzed intramolecular hydroamination reaction, and acridinium derivatives were demonstrated to be the optimal catalysts. Additionally, the synthesized tricyclic aza-andrographolide derivatives were found to inhibit human coronavirus with high potency.
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http://dx.doi.org/10.1021/acsomega.2c02979DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301648PMC
July 2022

Design, Synthesis, and Biological Evaluation of N-Acyl-Homoserine Lactone Analogs of Quorum Sensing in .

Front Chem 2022 8;10:948687. Epub 2022 Jul 8.

International Scientific and Technological Cooperation Base of Biopharmaceutical, School of Life Sciences, Institute of Microbiology, Lanzhou University, Lanzhou, China.

Quorum sensing plays a necessary role in the production of virulence factors and the formation of biofilm on . Thus, the development of inhibition of quorum sensing is one of the most promising methods to control bacterial infection and antibiotic resistance. In this work, nine novel AHL analogs were designed, synthesized, and evaluated as potential quorum sensing inhibitors. The results depicted that structural modifications have significant effects on quorum sensing inhibition activity of AHL molecules. Without inhibiting the growth of , 2-(4-bromophenyl)-N-(2-oxotetrapyridinefuran-3-yl) butanamide (compound no.10) showed the excellent performance in inhibiting biofilm formation and virulence factor production among all the compounds through robustly suppressing the expression of QS related genes. In a molecular docking study, compound no.10 exhibited a higher affinity toward LasR than other AHL analogs. In addition, compound no.10 also exhibits the best inhibition effect on virulence production in the infection model.
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http://dx.doi.org/10.3389/fchem.2022.948687DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9305322PMC
July 2022

The Association Between Hypertensive Disorders in Pregnancy and the Risk of Developing Chronic Hypertension.

Front Cardiovasc Med 2022 7;9:897771. Epub 2022 Jul 7.

Department of Obstetrics and Gynecology, Nanjing Maternity and Child Health Care Hospital, Women's Hospital of Nanjing Medical University, Nanjing, China.

Objective: This meta-analysis comprehensively evaluated the association between hypertensive disorders in pregnancy (HDP) and the risk of developing chronic hypertension and the associations between specific types of HDP, including preeclampsia (PE) and gestational hypertension (GH), and the risk of developing chronic hypertension.

Design: Systematic review and meta-analysis.

Data Sources: The PubMed, Embase and Cochrane Library databases were searched from inception to August 20, 2021.

Methods: Depending on heterogeneity, the combined odds ratio (OR) of the 95% confidence interval (CI) was obtained with a random-effects or fixed-effects model. We used meta-regression analysis to explore the sources of heterogeneity. We analyzed the OR value after adjusting for age and BMI at recruitment, prepregnancy BMI, age at first delivery, and other factors. Additionally, we evaluated the results of the subgroup analysis by the year of publication (< 2016, ≥ 2016), study design, sample size (< 500, ≥ 500), region (North and South America, Europe, and other regions) and NOS score (< 7, ≥ 7).

Results: Our systematic review and meta-analysis comprehensively explored the relationships between HDP, GH, and PE and chronic hypertension. Twenty-one articles that included 634,293 patients were included. The results of this systematic review and meta-analysis suggested that women with a history of HDP are almost 3.6 times more likely to develop chronic hypertension than those without a history of HDP, women with a history of GH are almost 6.2 times more likely to develop chronic hypertension than those without a history of GH, and women with a history of PE are almost 3.2 times more likely to develop chronic hypertension than those without a history of PE. In addition, we further calculated the probability of developing chronic hypertension among patients with HDP or PE after adjusting for age and BMI at recruitment, prepregnancy BMI, age at first delivery, and other factors. The results suggested that women with a history of HDP are almost 2.47 times more likely to develop chronic hypertension than those without a history of HDP and that women with a history of PE are almost 3.78 times more likely to develop chronic hypertension than those without a history of PE. People in Asian countries are more likely to develop chronic hypertension after HDP or PE, while American people are not at high relative risk.

Conclusion: These findings suggest that HDP, GH, and PE increase the likelihood of developing chronic hypertension. After adjustment for age and BMI at recruitment, prepregnancy BMI, age at first delivery, and other factors, patients with HDP or PE were still more likely to develop chronic hypertension. HDP may be a risk factor for chronic hypertension, independent of other risk factors. GH and PE, as types of HDP, may also be risk factors for chronic hypertension.

Systematic Review Registration: [www.ClinicalTrials.gov], identifier [CRD42021238599].
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http://dx.doi.org/10.3389/fcvm.2022.897771DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301072PMC
July 2022

Evaluating Tumor-Infiltrating Lymphocytes in Breast Cancer: The Role of Conventional Ultrasound and Contrast-Enhanced Ultrasound.

J Ultrasound Med 2022 Jul 21. Epub 2022 Jul 21.

Ultrasound Medical Center, Lanzhou University Second Hospital, Lanzhou, China.

Objectives: Tumor-infiltrating lymphocytes (TILs) have emerged as an efficient biomarker predicating treatment response and prognosis of breast cancer (BC). This study aimed to evaluate the association between conventional ultrasound and contrast-enhanced ultrasound (CEUS) imaging features with TILs levels in invasive BC patients.

Methods: We retrospectively included 267 women with invasive BC who had undergone conventional ultrasound and CEUS. Patients were divided into low (≤10%) and high (>10%) TILs groups. Conventional ultrasound and CEUS features were analyzed by two sonographers. The associations between the TILs levels and imaging features were evaluated.

Results: Of the 267 patients,122 with high TILs and 145 with low TILs levels. High TILs tumors were more likely to have a circumscribed margin, oval or round shape, and enhanced posterior echoes on ultrasonography (p < 0.05). In contrast, low TILs tumors were more likely to have an irregular shape, un-circumscribed, indistinct and spiculated margin (p < 0.05). In CEUS, high TILs tumors showed a more regular shape, clearer margin, more homogeneous enhancement and higher peak intensity (PI) value (p < 0.05). Logistic analysis indicated that shape, posterior features, PI, and enhanced homogeneity were independent predictors for high TILs tumors. The model combined the four independent predictors have a moderate performance in predicting high TILs tumors with AUC 0.79, sensitivity 0.72, and specificity 0.78.

Conclusions: Conventional ultrasound and CEUS features were associated with TILs levels in invasive BC. Consequently, the results suggested that preoperative conventional ultrasound and CEUS may be an useful non-invasive imaging biomarker for individualized treatment decisions.
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http://dx.doi.org/10.1002/jum.16058DOI Listing
July 2022

Human Umbilical Cord-Derived Mesenchymal Stem Cells Ameliorate Skin Aging of Nude Mice Through Autophagy-Mediated Anti-Senescent Mechanism.

Stem Cell Rev Rep 2022 Jul 21. Epub 2022 Jul 21.

The First Affiliated Hospital, Zhejiang Chinese Medical University, Hangzhou, China.

Skin aging is a currently irreversible process, affected by increased oxidative stress, activated cellular senescence, and lacked regeneration of the dermal layer. Mesenchymal stem cells (MSCs), such as human umbilical cord-derived MSCs (hucMSCs), have pro-regeneration and anti-aging potencies. To explore whether hucMSCs can be used to treat skin aging, this study employed skin-aging model of nude mice to conduct in vivo assays, including biochemical analysis of superoxide dismutase (SOD) and malondialdehyde (MDA), gross observation, histopathological observation, and immunohistochemical analysis. To clarify how hucMSCs work on skin aging, this study employed skin-aging model of human dermal fibroblasts (HDFs) to conduct in vitro assays by applying conditional medium of hucMSCs (CMM), including wound healing assay, senescence staining, flow cytometric oxidative detection, real time PCR, and western blot analysis. The in vivo data demonstrated that hucMSCs dose-dependently removed wrinkles, smoothed skin texture, and increased dermal thickness and collagen production of aged skin by reversing SOD and MDA levels and up-regulating Col-1 and VEGF expressions, indicating anti-oxidative and pro-regenerative effects against skin aging. The in vitro data revealed that hucMSCs significantly reversed the senescence of HDFs by promoting cell migration, inhibiting ROS production, and restoring the overexpressions of oxidative and senescent markers through paracrine mode of action, and the paracrine mechanism was mediated by the inhibition of autophagy. This study provided novel knowledge regarding the anti-aging efficacy and paracrine mechanism of hucMSCs on skin, making hucMSCs-based therapy a promising regime for skin aging treatment.
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http://dx.doi.org/10.1007/s12015-022-10418-9DOI Listing
July 2022

Abbreviated perioperative fasting management for elective fresh fracture surgery: guideline adherence analysis.

BMC Musculoskelet Disord 2022 Jul 20;23(1):688. Epub 2022 Jul 20.

Department of Orthopedic Trauma, Beijing Jishuitan Hospital, Beijing, 100035, China.

Background: Long-term fasting for elective surgery has been proven unnecessary based on established guidelines. Instead, preoperative carbohydrate loading 2 h before surgery and recommencing oral nutrition intake as soon as possible after surgery is recommended. This study was performed to analyze the compliance with and effect of abbreviated perioperative fasting management in patients undergoing surgical repair of fresh fractures based on current guidelines.

Methods: Patients with fresh fractures were retrospectively analyzed from the prospectively collected database about perioperative managements based on enhanced recovery of surgery (ERAS) from May 2019 to July 2019 at our hospital. A carbohydrate-enriched beverage was recommended up to 2 h before surgery for all surgical patients except those with contraindications. Postoperatively, oral clear liquids were allowed once the patients had regained full consciousness, and solid food was allowed 1 to 2 h later according to the patients' willingness. The perioperative fasting time was recorded and the patients' subjective comfort with respect to thirst and hunger was assessed using an interview-assisted questionnaire.

Results: In total, 306 patients were enrolled in this study. The compliance rate of preoperative carbohydrate loading was 71.6%, and 93.5% of patients began ingestion of oral liquids within 2 h after surgery. The median (interquartile range) preoperative fasting time for liquids and solids was 8 (5.2-12.9) and 19 (15.7-22) hours, respectively. The median postoperative fasting time for liquids and solids was 1 (0.5-1.9) and 2.8 (2.2-3.5) hours, respectively. A total of 70.3% and 74.2% of patients reported no thirst and hunger during the perioperative period, respectively. Logistic regression analysis showed that the preoperative fasting time for liquids was an independent risk factor for perioperative hunger. No risk factor was identified for perioperative thirst. No adverse events such as aspiration pneumonia or gastroesophageal reflux were observed.

Conclusions: In this study of a real clinical practice setting, abbreviated perioperative fasting management was carried out with high compliance in patients with fresh fractures. The preoperative fasting time should be further shortened to further improve patients' subjective comfort.
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http://dx.doi.org/10.1186/s12891-022-05574-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9297612PMC
July 2022

Latency period of PROM at term and the risk of neonatal infectious diseases.

Sci Rep 2022 Jul 18;12(1):12275. Epub 2022 Jul 18.

Faculty of Pediatrics, Chinese PLA General Hospital, Beijing, China.

To find the risk of time thresholds of PROM for infectious diseases of term neonates. A multi-center prospective cohort study including pregnancies with PROM at term with a single fetus were conducted. Time thresholds of the duration from PROM to delivery were examined in 2-h increments to assess the rates of infectious neonatal diseases. 7019 pregnancies were included in the study. Neonatal pneumonia and sepsis were most frequent infectious diseases in neonates born from mother with PROM at term. Rates of early-onset pneumonia varied significantly when comparing length of time of PROM greater than 16 h vs. less than 16 h (for EOP in 3 days of life, adjusted OR 1.864, 95% CI 1.159 ~ 2.997, p = 0.010; for EOP in 7 days of life, adjusted OR 1.704, 95% CI 1.104 ~ 2.628, p = 0.016). Neonates born from mother of whom the length of time from PROM to delivery ≥ 16 h were at a higher risk of acquiring EOP.
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http://dx.doi.org/10.1038/s41598-022-16593-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9293978PMC
July 2022

The role of influenza in the era of COVID-19: Can we forget it?

Biosci Trends 2022 Jul 17. Epub 2022 Jul 17.

Department of Infectious Diseases, National Clinical Research Center for Infectious Diseases, the Third People's Hospital of Shenzhen, Shenzhen, China.

COVID-19 has been a topic of interest since a pandemic struck in 2019. The morbidity of influenza tended to decrease due to the measures to prevent COVID-19. Indeed, influenza seems to be "ignored" in this era of COVID-19. However, influenza has not disappeared from the scene. Presented here are two examples of recent influenza epidemics in China and Australia. Possible interactions between COVID-19 and influenza are discussed. Measures against COVID-19 may reduce contact with influenza, subsequently reducing adaptive immunity against influenza in the general population. Influenza might not be center stage right now, but insufficient adaptive immunity in the population may potentially trigger a future influenza pandemic. Coinfection with COVID-19 and influenza might potentially be a thorny problem. Hence, influenza cannot be ignored. Governments around the world should take measures to prepare for a possible influenza pandemic in the future.
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http://dx.doi.org/10.5582/bst.2022.01297DOI Listing
July 2022
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