Publications by authors named "Ting Fu"

228 Publications

Association of the Gene Polymorphism with the Risk of Atrial Fibrillation and Warfarin Anticoagulation Therapy.

Genet Test Mol Biomarkers 2021 Sep 9. Epub 2021 Sep 9.

Department of Cardiology, Yiwu Central Hospital, Yiwu, China.

To study the associations of a single nucleotide polymorphisms (SNP) of the myosin heavy chain 6 () gene with the risk of atrial fibrillation (AF) and warfarin anticoagulation therapy. Sanger sequencing was employed to analyze the genotypes of the gene's rs28730771, rs365990, and rs2277473 loci in 243 AF patients and 243 non-AF patients (control group) selected according to the age and sex of AF patients at a 1:1 ratio. Multiple logistic regression was used to analyze the risk factors in AF. SHEsis was adopted to analyze the association between rs28730771, rs365990, rs2277473 haplotypes and susceptibility to AF. The average weekly dose of warfarin administered to AF patients with different genotypes was compared. The T allele at rs28730771 of the gene (odds ratio [OR] = 2.82, 95% confidence interval [CI]: 1.73-4.59,  < 0.01), the G allele at rs365990 (OR = 1.65, 95% CI: 1.22-2.24,  < 0.01) and the T allele at rs2277473 (OR = 1.91, 95% CI: 1.25-2.91,  < 0.01) were significantly associated with an elevated risk of AF. The results of a logistic regression analysis demonstrated that hypertension, smoking, drinking, family history of stroke, as well as the genotypes at the rs28730771, rs365990, and rs2277473 loci were all risk factors in AF ( < 0.05). The CAG haplotype for the three SNPs was associated with a reduced risk of AF susceptibility (OR = 0.61, 95% CI: 0.46-0.81,  < 0.01), and the CGG haplotype was related to an increased risk of AF (OR = 1.49, 95% CI: 1.07-2.06,  = 0.02). The doses of warfarin used in AF patients with different genotypes at the rs28730771, rs365990, and rs2277473 were significantly different ( < 0.05). The three SNPs (rs28730771, rs365990, and rs2277473) of the gene loci were significantly associated with the risk of AF susceptibility and the dose of warfarin anticoagulant therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1089/gtmb.2021.0025DOI Listing
September 2021

A new paradigm for artesunate anticancer function: considerably enhancing the cytotoxicity via conjugating artesunate with aptamer.

Signal Transduct Target Ther 2021 Sep 10;6(1):327. Epub 2021 Sep 10.

Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, PR China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41392-021-00671-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8429419PMC
September 2021

Protein Enrichment of Donor Breast Milk and Impact on Growth in Very Low Birth Weight Infants.

Nutrients 2021 Aug 20;13(8). Epub 2021 Aug 20.

Perinatal Institute, Division of Neonatology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, USA.

Protein content is often inadequate in donor breast milk (DBM), resulting in poor growth. The use of protein-enriched target-pooled DBM (DBM+) has not been examined. We compared three cohorts of very low birth weight (VLBW) infants, born ≤ 1500 g: DBM cohort receiving > 1-week target-pooled DBM (20 kcal/oz), MBM cohort receiving ≤ 1-week DBM, and DBM+ cohort receiving > 1-week DBM+. Infants followed a standardized feeding regimen with additional fortification per clinical discretion. Growth velocities and z-scores were calculated for the first 4 weeks ( = 69 for DBM, 71 for MBM, 70 for DBM+) and at 36 weeks post-menstrual age ( = 58, 64, 59, respectively). In total, 60.8% MBM infants received fortification >24 kcal/oz in the first 30 days vs. 78.3% DBM and 77.1% DBM+. Adjusting for SGA, length velocity was greater with DBM+ than DBM in week 1. Average weight velocity and z-score change were improved with MBM compared to DBM and DBM+, but length z-score decreased similarly across all groups. Incidences of NEC and feeding intolerance were unchanged between eras. Thus, baseline protein enrichment appears safe in stable VLBW infants. Weight gain is greatest with MBM. Linear growth comparable to MBM is achievable with DBM+, though the overall length trajectory remains suboptimal.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/nu13082869DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401419PMC
August 2021

Short-Term High Fructose Intake Impairs Diurnal Oscillations in the Murine Cornea.

Invest Ophthalmol Vis Sci 2021 Aug;62(10):22

International Ocular Surface Research Center, Institute of Ophthalmology and Key Laboratory for Regenerative Medicine, Jinan University, Guangzhou, China.

Purpose: Endogenous and exogenous stressors, including nutritional challenges, may alter circadian rhythms in the cornea. This study aimed to determine the effects of high fructose intake (HFI) on circadian homeostasis in murine cornea.

Methods: Corneas of male C57BL/6J mice subjected to 10 days of HFI (15% fructose in drinking water) were collected at 3-hour intervals over a 24-hour circadian cycle. Total extracted RNA was subjected to high-throughput RNA sequencing. Rhythmic transcriptional data were analyzed to determine the phase, rhythmicity, unique signature, metabolic pathways, and cell signaling pathways of transcripts with temporally coordinated expression. Corneas of HFI mice were collected for whole-mounted techniques after immunofluorescent staining to quantify mitotic cell number in the epithelium and trafficking of neutrophils and γδ-T cells to the limbal region over a circadian cycle.

Results: HFI significantly reprogrammed the circadian transcriptomic profiles of the normal cornea and reorganized unique temporal and clustering enrichment pathways, but did not affect core-clock machinery. HFI altered the distribution pattern and number of corneal epithelial mitotic cells and enhanced recruitment of neutrophils and γδ-T cell immune cells to the limbus across a circadian cycle. Cell cycle, immune function, metabolic processes, and neuronal-related transcription and associated pathways were altered in the corneas of HFI mice.

Conclusions: HFI significantly reprograms diurnal oscillations in the cornea based on temporal and spatial distributions of epithelial mitosis, immune cell trafficking, and cell signaling pathways. Our findings reveal novel molecular targets for treating pathologic alterations in the cornea after HFI.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1167/iovs.62.10.22DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8383902PMC
August 2021

Ratiometric fluorescence and smartphone dual-mode detection of glutathione using carbon dots coupled with Ag-triggered oxidation of o-phenylenediamine.

Nanotechnology 2021 Aug 13;32(44). Epub 2021 Aug 13.

College of Chemistry and Materials Science, Anhui Key Laboratory of Chemo/Biosensing, The Key Laboratory of Functional Molecular Solids, Ministry of Education, Anhui Laboratory of Molecule-based Materials, Anhui Normal University, Wuhu 241000, People's Republic of China.

Developing ratiometric fluorescence and smartphone dual-mode bioanalysis methods is important but challenging. A ratiometric fluorescence method for determining glutathione (GSH) using carbon dots (CDs) and Ag-triggered o-phenylenediamine (OPD) oxidation is described here. Agoxidizes OPD to give 2,3-diaminophenazine (oxOPD), which effectively quenches CD fluorescence at 436 nm through the inner filter effect and causes a new emission peak at 561 nm. GSH chelates with Agand prevents the Agoxidizing OPD and therefore effectively preserves CD emission at 436 nm (blue) and allows only weak oxOPD fluorescence at 561 nm (orange) to occur. The oxOPD to CD fluorescence intensity ratio decreased linearly as the GSH concentration increased in the range 0-150 nM, and the detection limit was 15 nM. The ratiometric fluorescence probe lit with an ultraviolet lamp clearly changed color from orange to blue as the GSH concentration increased. An image was acquired using a smartphone camera and converted into digital values. The blue and red channel ratio was calculated and used to quantify GSH. The method therefore allows dual-mode detection of GSH.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1088/1361-6528/ac1978DOI Listing
August 2021

G-Quadruplex-Induced Liquid-Liquid Phase Separation in Biomimetic Protocells.

J Am Chem Soc 2021 Jul 16;143(29):11036-11043. Epub 2021 Jul 16.

Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, Hunan 410082, China.

Biomolecular condensates comprised of specific proteins and nucleic acids are now recognized as one of the key organizing mechanisms in eukaryotic cells. However, the specific roles played by the nucleic acid secondary structure and sequence in biomolecular phase separation are still not clear. Here, utilizing giant membrane vesicles (GMVs) as a protocell model, we found that single-stranded DNA (ssDNA) with a parallel G-quadruplex structure could functionally cooperate with a G-quadruplex-binding protein to form speckle-like puncta inside the GMVs. The clustering behavior is dependent on the structural diversity of G-quadruplexes, and the reversible clustering behavior implicated a new pathway in dynamically regulating the formation of biomolecular condensates. This finding represents a potential link between G-quadruplex-binding proteins and the resulting G-quadruplex-mediated biomolecular phase separation, which would gain insight into a wide range of biological processes associated with nucleic acid-modulated phase separation inside living cells.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/jacs.1c03627DOI Listing
July 2021

Approaches to tuning the exceptional point of PT-symmetric double ridge stripe lasers.

Opt Express 2021 Jun;29(13):20440-20448

Electrically injected Parity-time (PT)-symmetric double ridge stripe semiconductor lasers lasing at 980 nm range are designed and measured. The spontaneous PT-symmetric breaking point or exceptional point (EP) of the laser is tuned below or above the lasing threshold by means of varying the coupling constant or the mirror loss. The linewidth of the optical spectrum of the PT-symmetric laser is narrowed, compared with that of traditional single ridge (SR) laser and double ridge (DR) laser. Furthermore, the far field pattern of the PT-symmetric laser with EP below the lasing threshold is compared with that of the PT-symmetric laser with EP above the lasing threshold experimentally. It is found that when the laser start to lase, the former is single-lobed while the latter is double-lobed. when the current continues to increase, the former develops into double lobe directly while the latter first develops into single lobe and then double lobe again.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/OE.423266DOI Listing
June 2021

Integrated Battery-Capacitor Electrodes: Pyridinic N-Doped Porous Carbon-Coated Abundant Oxygen Vacancy Mn-Ni-Layered Double Oxide for Hybrid Supercapacitors.

ACS Appl Mater Interfaces 2021 Jul 14;13(29):34374-34384. Epub 2021 Jul 14.

Key Laboratory for Magnetism and Materials of MOE, School of Physical Science and Technology, Lanzhou University, 730000 Lanzhou, China.

Integrating the battery behavior and supercapacitor behavior in a single electrode to obtain better electrochemical performance has been widely researched. However, there is still a lack of research studies on an integrated battery-capacitor supercapacitor electrode (BatCap electrode). In this work, an integrated BatCap electrode porous carbon-coated Mn-Ni-layered double oxide (Mn-Ni LDO-C) was fabricated successfully using controllable heat treatment of polypyrrole-precoated Mn-Ni-layered double hydroxide (Mn-Ni [email protected]). This Mn-Ni LDO-C electrode was grown on Ni foam directly and possessed a hierarchical structure that consisted of a pyridinic N (N-6)-doped porous carbon shell and a Mn-Ni LDO core within abundant oxygen vacancies. Benefiting from the synergistic effect of N-6-doped porous carbon and increased oxygen vacancies, Mn-Ni LDO-C exhibited excellent electrochemical performance. The capacity of Mn-Ni LDO-C reached 2.36 C cm (1478.1 C g) at 1 mA cm and remained at 92.1% of the initial capacity after 5000 cycles at a current density of 20 mA cm. The aqueous battery-supercapacitor hybrid device Mn-Ni LDO-C//active carbon (Mn-Ni LDO-C//AC) also presented superior cycle stability: it retained 85.3% of the original capacity after 5000 cycles at 2 A g. Meanwhile, Mn-Ni LDO-C//AC could work normally under a wider potential window (2.0 V), so that the device held the highest energy density of 78.2 Wh kg at a power density of 499.7 W kg and retained 39.1 Wh kg at the highest power density of 31.3 kW kg. Two Mn-Ni LDO-C//AC devices connected in series could light a light-emitting diode (LED) bulb easily and keep the LED brightly illuminated for more than 10 min. In general, this work synthesized an integrated BatCap electrode Mn-Ni LDO-C; the integrated electrode exhibited high electrochemical performance, thus has a promising application prospect in the field of energy storage.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.1c08699DOI Listing
July 2021

Numerical and Experimental Investigations of Micro Thermal Performance in a Tube with Delta Winglet Pairs.

Micromachines (Basel) 2021 Jun 30;12(7). Epub 2021 Jun 30.

Department of Mechanical & Mechatronics Engineering, University of Waterloo, Waterloo, ON N2L 3G1, Canada.

In this research, a novel vortex generator (VG) is presented. The experimental and numerical investigations were carried out to study the micro thermal-hydraulic performance in a heated tube. The numerical results showed that the fluid in the core flow region and the near-wall region was fully mixed because of the longitudinal vortices created by the vortex generators. In addition, the experimental results showed that the heat transfer coefficient () decreased with the increasing pitch ratio () value, while the friction coefficient exhibited the opposite trend. With the increasing ration angle () numbers, the values decreased while the numbers increased. In addition, the maximum and minimum values of the fraction ratio were 1.66 and 4.27, while these values of the Nusselt number ratio were 1.24 and 1.83. The maximum thermal enhancement factor (TEF) was 1.21 when = 0.5, = 0° and Re = 9090. The heat transfer enhancement mechanism of the vortex generator is explained from the microscopic point of view.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/mi12070786DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8306842PMC
June 2021

Logic-Gated Cell-Derived Nanovesicles via DNA-Based Smart Recognition Module.

ACS Appl Mater Interfaces 2021 Jul 23;13(26):30397-30403. Epub 2021 Jun 23.

Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, Hunan 410082, China.

Engineering cell-derived nanovesicles with active-targeting ligands is an important strategy to enhance the targeting efficiency. However, the enhanced binding capability to targeting cells also leads to the binding with nontarget cells that share the same biomarkers. DNA-based logic gate is a kind of molecular system that responds to chemical inputs by generating output signals, and the relationship between the input and the output is based on a certain logic. Thus, the DNA-based logic gate could provide a new approach to improve the delivery efficiency of the nanovesicle. In this work, we developed a DNA logic-gated module that coupled two tumor cell-targeting factors (e.g., low pH and a tumor cell biomarker) in a Boolean manner. Immobilization of this module on the surface of the nanovesicle enables the nanovesicle to sense tumor cell-targeting factors and regard these cues as inputs AND logic gate. With the guide of DNA-based logic gate, gold carbon dots (GCDs) encapsulated within nanovesicles were delivered into target cells, and then the intracellular redox status variation was reflected by fluorescence change of GCDs. Overall, we developed DNA logic-gated nanovesicles that contract different targeting factors into a unique tag for target cells. This facile functionalization strategy can pave the way for constructing smart nanovesicles and would broaden their application in the field of precision medicine and personalized treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.1c07632DOI Listing
July 2021

Metal-Organic Framework Nanocarriers for Drug Delivery in Biomedical Applications.

Nanomicro Lett 2020 May 2;12(1):103. Epub 2020 May 2.

Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, 410082, People's Republic of China.

Investigation of metal-organic frameworks (MOFs) for biomedical applications has attracted much attention in recent years. MOFs are regarded as a promising class of nanocarriers for drug delivery owing to well-defined structure, ultrahigh surface area and porosity, tunable pore size, and easy chemical functionalization. In this review, the unique properties of MOFs and their advantages as nanocarriers for drug delivery in biomedical applications were discussed in the first section. Then, state-of-the-art strategies to functionalize MOFs with therapeutic agents were summarized, including surface adsorption, pore encapsulation, covalent binding, and functional molecules as building blocks. In the third section, the most recent biological applications of MOFs for intracellular delivery of drugs, proteins, and nucleic acids, especially aptamers, were presented. Finally, challenges and prospects were comprehensively discussed to provide context for future development of MOFs as efficient drug delivery systems.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s40820-020-00423-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7770922PMC
May 2020

A Cluster Transmission of Coronavirus Disease 2019 and the Prevention and Control Measures in the Early Stage of the Epidemic in Xi'an, China, 2020.

Med Sci Monit 2021 Jun 14;27:e929701. Epub 2021 Jun 14.

Department of Epidemiology, Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, School of Public Health, Air Force Medical University, Xi'an, Shaanxi, China (mainland).

BACKGROUND At the beginning of the COVID-19 pandemic, a cluster outbreak caused by an imported case from Hubei Province was reported in Xi'an City, Shaanxi Province, China. Ten patients from 2 families and 1 hospital were involved in the transmission. MATERIAL AND METHODS We conducted an epidemiological investigation to identify the cluster transmission of COVID-19. The demographic, epidemiological, clinical, laboratory, and cluster characteristics were described and analyzed. RESULTS From January 27 to February 13, 2020, a total of 10 individuals were confirmed to be infected with SARS-CoV-2 by the nucleic acid testing of nasopharyngeal swabs from 2 families and 1 hospital. Among the confirmed cases, 7 had atypical clinical symptoms and 3 were asymptomatic. The median times from onset to diagnosis and to discharge were 3.5 days (range, 1-5 days) and 19.5 days (range, 16-38 days), respectively. There were 4 patients whose exposure dates were 1, 3, 3, and 2 days earlier than the onset dates of their previous-generation cases, respectively. Four prevention and control measures were effectively used to interrupt the disease transmission. CONCLUSIONS SARS-CoV-2 can be easily transmitted within families and in hospitals, and asymptomatic patients could act as a source of disease transmission. The results of this outbreak at the early epidemic stage support the recommendation that individuals with confirmed COVID-19 and all their close contacts should be subjected to medical quarantined observation and nucleic acid screening as early as possible, even if they do not have any symptoms. Meanwhile, people in high-risk areas should improve their protective measures.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.12659/MSM.929701DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212697PMC
June 2021

Sensitive detection of picric acid in an aqueous solution using fluorescent nonconjugated polymer dots as fluorescent probes.

Nanotechnology 2021 Jun 11;32(35). Epub 2021 Jun 11.

College of Chemistry and Materials Science, Anhui Key Laboratory of Chemo/Biosensing, The Key Laboratory of Functional Molecular Solids, Ministry of Education, Anhui Laboratory of Molecule-based Materials, Anhui Normal University, Wuhu 241000, People's Republic of China.

Nonconjugated polymer dots (NPDs) were successfully used as fluorescent probes to selectively and sensitively detect picric acid (PA). The NPDs were prepared from polyethylenimine and 1,4-phthalaldehyde under mild conditions and had excitation and emission maxima of 351 and 474 nm, respectively. Fluorescence of the NPDs was efficiently quenched by PA through the inner filter effect because of the overlapping PA absorption band and NPD excitation spectrum. The NPDs allowed PA to be determined with a high degree of sensitivity. The linear range was 0-140M and the detection limit was 0.5M. The work involved developing a novel method for synthesizing NPDs and a promising platform for determining PA in environmental media.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1088/1361-6528/ac04d1DOI Listing
June 2021

Modification of COL1A1 in Autologous Adipose Tissue-Derived Progenitor Cells Rescues the Bone Phenotype in a Mouse Model of Osteogenesis Imperfecta.

J Bone Miner Res 2021 08 18;36(8):1521-1534. Epub 2021 May 18.

Department of Genetics, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, People's Republic of China.

Osteogenesis imperfecta (OI) is a congenital genetic disorder mainly manifested as bone fragility and recurrent fracture. Mutation of COL1A1/COL1A2 genes encoding the type I collagen are most responsible for the clinical patients. Allogenic mesenchymal stem cells (MSCs) provide the potential to treat OI through differentiation into osteoblasts. Autologous defective MSCs have not been utilized in OI treatment mainly because of their impaired osteogenesis, but the latent mechanism has not been well understood. Here, the relative signaling abnormality of adipose-derived mesenchymal stem cells (ADSCs) isolated from OI type I mice (Col1a1 mice) was explored. Autologous ADSCs transfected by retrovirus carrying human COL1A1 gene was first utilized in OI therapy. The results showed that decreased activity of Yes-associated protein (YAP) due to hyperactive upstream Hippo kinases greatly contributed to the weakened bone-forming capacity of defective ADSCs. Recovered collagen synthesis of autologous ADSCs by COL1A1 gene modification normalized Hippo/YAP signaling and effectively rescued YAP-mediated osteogenesis. And the COL1A1 gene engineered autologous ADSCs efficaciously improved the microstructure, enhanced the mechanical properties and promoted bone formation of Col1a1 mice after femoral bone marrow cavity delivery and might serve as an alternative source of stem cells in OI treatment. © 2021 American Society for Bone and Mineral Research (ASBMR).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/jbmr.4326DOI Listing
August 2021

Rapid One-Step Detection of Viral Particles Using an Aptamer-Based Thermophoretic Assay.

J Am Chem Soc 2021 05 4;143(19):7261-7266. Epub 2021 May 4.

Institute of Cancer and Basic Medicine (ICBM), Chinese Academy of Sciences, The Cancer Hospital of the University of Chinese Academy of Sciences, Hangzhou, Zhejiang 310018, China.

Rapid and sensitive identification of viral pathogens such as SARS-CoV-2 is a critical step to control the pandemic disease. Viral antigen detection can compete with gold-standard PCR-based nucleic acid diagnostics in terms of better reflection of viral infectivity and reduced risk of contamination from enzymatic amplification. Here, we report the development of a one-step thermophoretic assay using an aptamer and polyethylene glycol (PEG) for direct quantitative detection of viral particles. The assay relies on aptamer binding to the spike protein of SARS-CoV-2 and simultaneous accumulation of aptamer-bound viral particles in laser-induced gradients of temperature and PEG concentration. Using a pseudotyped lentivirus model, a limit of detection of ∼170 particles μL (26 fM of the spike protein) is achieved in 15 min without the need of any pretreatment. As a proof of concept, the one-step thermophoretic assay is used to detect synthetic samples by spiking viral particles into oropharyngeal swabs with an accuracy of 100%. The simplicity, speed, and cost-effectiveness of this thermophoretic assay may expand the diagnostic tools for viral pathogens.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/jacs.1c02929DOI Listing
May 2021

An integrated methodology for real-time driving risk status prediction using naturalistic driving data.

Accid Anal Prev 2021 Jun 23;156:106122. Epub 2021 Apr 23.

The Key Laboratory of Road and Traffic Engineering, Ministry of Education, Tongji University, Shanghai, 201804, China; College of Transportation Engineering, Tongji University, 4800 Cao'an Highway, Shanghai, 201804, China. Electronic address:

Real-time driving risk status prediction is critical for developing proactive traffic intervention strategies and enhance driving safety. However, the optimal observation time window length and prediction time window length, which should be the prerequisite for the timeliness and accuracy of real-time driving risk status prediction model, have been rarely explored in previous studies. In this study, a methodology which integrates driving risk status identification, rolling time window-based feature extraction, real-time driving risk status prediction and driving risk influencing factors analysis was proposed to accurately evaluate and predict real-time driving risk status. The methodology was tested based on 1,440 car-following events from Shanghai Naturalistic Driving Study. Results show that four driving risk statuses (safe, low-risk, median-risk and high-risk) are most appropriate to establish risk labelling criteria. In addition, results from driving risk status prediction show that when the observation time window length is 0.5 s, the accuracy rate of predicting medium-risk or high-risk status occurring in the next 0.7 s is higher than 85 % using multi-layer perceptron model. Meanwhile, the results from the analysis of influencing factors show that the input variables related to the risk status score higher in the ranking of feature importance. A part from that, speed difference, headway distance, speed and acceleration are still important in predicting driving risk status. The proposed methods in this paper can be applied in connected and autonomous vehicle (CAV) to reduce driver cognitive workload and hence improve driving safety fed with naturalistic driving data collected using in-vehicle systems.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.aap.2021.106122DOI Listing
June 2021

Efficacy, safety and complications of autologous fat grafting to the eyelids and periorbital area: A systematic review and meta-analysis.

PLoS One 2021 1;16(4):e0248505. Epub 2021 Apr 1.

Department of Epidemiology, Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, School of Public Health, The Fourth Military Medical University, Xi'an, China.

Background: In recent years, autologous fat grafting (AFG), also known as fat transfer or lipofilling, has been widely performed for periorbital rejuvenation and defect correction, although the evidence regarding its efficacy and safety is still lacking. Besides, with respect to the periorbital region, it is invariably the earliest appearance area of the facial aging phenomenon. Therefore, a systematic review and meta-analysis is needed to evaluate the efficacy and safety of this technique.

Methods: A literature search was performed in PubMed, Embase, and the Cochrane library databases on November 20, 2020, adhering to the PRISMA guidelines, to identify all relevant articles. Then, a data extraction and standardization process was performed to assess all outcome data. Ultimately, the data were assessed using a random effects regression model with comprehensive meta-analysis software.

Results: Thirty-nine studies consisting of 3 cohorts and 36 case series with a total of 4046 cases were included. Meta-analysis revealed a relatively high satisfaction rate of 90.9% (95% CI, 86.4%-94.0%). Frequent complications in 4046 patients receiving AFG were edema, chemosis, and contour irregularity, with an overall complication rate of 7.9% (95% CI, 4.8%-12.8%).

Conclusion: This systematic review and meta-analysis showed that AFG for rejuvenation of eyelids and periorbital area provided a high satisfaction rate and did not result in severe complications. Therefore, AFG might be performed safely for periorbital rejuvenation and reconstruction.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0248505PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8016360PMC
April 2021

The clinical classification of patients with COVID-19 pneumonia was predicted by Radiomics using chest CT.

Medicine (Baltimore) 2021 Mar;100(12):e25307

Department of Radiology, PLA Central Theater General Hospital, Wuhan, Hubei, China.

Abstract: In 2020, the new type of coronal pneumonitis became a pandemic in the world, and has firstly been reported in Wuhan, China. Chest CT is a vital component in the diagnostic algorithm for patients with suspected or confirmed COVID-19 infection. Therefore, it is necessary to conduct automatic and accurate detection of COVID-19 by chest CT.The clinical classification of patients with COVID-19 pneumonia was predicted by Radiomics using chest CT.From the COVID-19 cases in our institution, 136 moderate patients and 83 severe patients were screened, and their clinical and laboratory data on admission were collected for statistical analysis. Initial CT Radiomics were modeled by automatic machine learning, and diagnostic performance was evaluated according to AUC, TPR, TNR, PPV and NPV of the subjects. At the same time, the initial CT main features of the two groups were analyzed semi-quantitatively, and the results were statistically analyzed.There was a statistical difference in age between the moderate group and the severe group. The model cohort showed TPR 96.9%, TNR 99.1%, PPV98.4%, NPV98.2%, and AUC 0.98. The test cohort showed TPR 94.4%, TNR100%, PPV100%, NPV96.2%, and AUC 0.97. There was statistical difference between the two groups with grade 1 score (P = .001), the AUC of grade 1 score, grade 2 score, grade 3 score and CT score were 0.619, 0.519, 0.478 and 0.548, respectively.Radiomics' Auto ML model was built by CT image of initial COVID -19 pneumonia, and it proved to be effectively used to predict the clinical classification of COVID-19 pneumonia. CT features have limited ability to predict the clinical typing of Covid-19 pneumonia.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000025307DOI Listing
March 2021

Quantitative analysis of internal components of the human crystalline lens during accommodation in adults.

Sci Rep 2021 03 23;11(1):6688. Epub 2021 Mar 23.

Department of Ophthalmology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Jiefangroad1095#, Wuhan, 430030, Hubei, China.

To quantitatively analyze changes in the inner components of the human crystalline lens during accommodation in adults. Eyes of 23 subjects were sequentially examined using CASIA2 Optical Coherence Tomography under 0D, - 3D and - 6D accommodation states. The anterior chamber depth (ACD), anterior and posterior crystalline lens radius of the curvature (ALRC and PLRC) were obtained using built-in software. The lens thickness (LT), lenticular nucleus thickness (NT), anterior cortex thickness (ACT), posterior cortex thickness (PCT), anterior and posterior lenticular nucleus radius of the curvature (ANRC and PNRC), anterior and posterior lenticular nucleus vertex (ANV and PNV) were quantified manually with the Image-pro plus software. During accommodation, the ACD became significantly shallower and LT significantly increased. For changes in the lens, the ALRC decreased by an average magnitude (related to accommodative stimuli) 0.44 mm/D, and PLRC decreased 0.09 mm/D. There was no difference for the ACT and PCT in different accommodation states. For lenticular nucleus response, NT increased on average by 30 μm/D. Both the ANRC and PNRC decreased on average by 212 μm/D and 115 μm/D respectively. The ANV moved forward on average by 0.07 mm under - 3D accommodative stimuli and 0.16 mm for - 6D. However, there was no statistically significant difference between different accommodation states in the PNV movement. Under accommodation stimulation, lens thickness changed mainly due to the lenticular nucleus, but not the cortex. For the lenticular nucleus, both the ANRC and PNRC decreased and ANRC changed the most. The anterior surface of the nucleus moved forward while the posterior surface of the nucleus moved backward but only slightly.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-021-86007-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7987977PMC
March 2021

Umbilical cord blood-derived microglia-like cells to model COVID-19 exposure.

Transl Psychiatry 2021 03 19;11(1):179. Epub 2021 Mar 19.

Obstetrics, Gynecology, and Reproductive Biology, Harvard Medical School, Boston, MA, USA.

Microglia, the resident brain immune cells, play a critical role in normal brain development, and are impacted by the intrauterine environment, including maternal immune activation and inflammatory exposures. The COVID-19 pandemic presents a potential developmental immune challenge to the fetal brain, in the setting of maternal SARS-CoV-2 infection with its attendant potential for cytokine production and, in severe cases, cytokine storming. There is currently no biomarker or model for in utero microglial priming and function that might aid in identifying the neonates and children most vulnerable to neurodevelopmental morbidity, as microglia remain inaccessible in fetal life and after birth. This study aimed to generate patient-derived microglial-like cell models unique to each neonate from reprogrammed umbilical cord blood mononuclear cells, adapting and extending a novel methodology previously validated for adult peripheral blood mononuclear cells. We demonstrate that umbilical cord blood mononuclear cells can be used to create microglial-like cell models morphologically and functionally similar to microglia observed in vivo. We illustrate the application of this approach by generating microglia from cells exposed and unexposed to maternal SARS-CoV-2 infection. Our ability to create personalized neonatal models of fetal brain immune programming enables non-invasive insights into fetal brain development and potential childhood neurodevelopmental vulnerabilities for a range of maternal exposures, including COVID-19.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41398-021-01287-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7976669PMC
March 2021

Exploring two-photon optogenetics beyond 1100 nm for specific and effective all-optical physiology.

iScience 2021 Mar 12;24(3):102184. Epub 2021 Feb 12.

Institute of Pathophysiology, University Medical Center Mainz, Hanns-Dieter-Hüsch-Weg 19, D-55128 Mainz, Germany.

Two-photon (2-P) all-optical approaches combine 2-P calcium imaging and 2-P optogenetic modulations. Here, firstly, we combined juxtacellular recordings and GCaMP6f-based 2-P calcium imaging in mouse visual cortex to tune our detection algorithm towards a 100% specific identification of action potential-related calcium transients. Secondly, we minimized photostimulation artifacts by using extended-wavelength-spectrum laser sources for optogenetic stimulation. We achieved artifact-free all-optical experiments performing optogenetic stimulation from 1100 nm to 1300 nm. Thirdly, we determined the spectral range for maximizing efficacy until 1300 nm. The rate of evoked transients in GCaMP6f/C1V1-co-expressing cortical neurons peaked already at 1100 nm. By refining spike detection and defining 1100 nm as the optimal wavelength for artifact-free and effective GCaMP6f/C1V1-based all-optical physiology, we increased the translational value of these approaches, e.g., for the development of network-based therapies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.isci.2021.102184DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921810PMC
March 2021

Functional Aptamer-Embedded Nanomaterials for Diagnostics and Therapeutics.

ACS Appl Mater Interfaces 2021 Mar 17;13(8):9542-9560. Epub 2021 Feb 17.

The Cancer Hospital of the University of Chinese Academy of Sciences, Institute of Basic Medicine and Cancer, Chinese Academy of Sciences, Hangzhou 310022, Zhejiang, China.

In the past decades, various nanomaterials with unique properties have been explored for bioapplications. Meanwhile, aptamers, generated from the systematic evolution of ligands by exponential enrichment technology, are becoming an indispensable element in the design of functional nanomaterials because of their small size, high stability, and convenient modification, especially endowing nanomaterials with recognition capability to specific targets. Therefore, the incorporation of aptamers into nanomaterials offers an unprecedented opportunity in the research fields of diagnostics and therapeutics. Here, we focus on recent advances in aptamer-embedded nanomaterials for bioapplications. First, we briefly introduce the properties of nanomaterials that can be functionalized with aptamers. Then, the applications of aptamer-embedded nanomaterials in cellular analysis, imaging, targeted drug delivery, gene editing, and cancer diagnosis/therapy are discussed. Finally, we provide some perspectives on the challenges and opportunities that have arisen from this promising area.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.0c19562DOI Listing
March 2021

Alterations in the intestinal microbiome and mental health status of workers in an underground tunnel environment.

BMC Microbiol 2021 01 6;21(1). Epub 2021 Jan 6.

Department of Epidemiology, Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, School of Public Health, Air Force Medical University, Xi'an, 710032, People's Republic of China.

Background: Working in an underground tunnel environment is unavoidable in professions such as miners and tunnel workers, and there is a concern about the health of these workers. Few studies have addressed alterations in the intestinal microbiome of workers within that environment.

Results: Fecal samples were collected from the workers before they entered the tunnel (baseline status, BS) and after they left the tunnel (exposed status, ES), respectively (a time period of 3 weeks between them). We analyzed 16S rRNA sequencing to show the changes in microbial composition and self-evaluation of mental health questionnaire was also performed. The results showed that Shannon and Simpson indices decreased significantly from BS to ES. A higher abundance was found in the phylum Actinobacteria, classes Actinobacteria and Deltaproteobacteria, orders Bifidobacteriales, Coriobacteriales, and Desulfovibrionales, families Bifidobacteriaceae, Peptostreptococcaceae, Coriobacteriaceae, Clostridiaceae_1, Desulfovibrionaceae, Pseudomonadaceae, and Microbacteriaceae, and genera Bifidobacterium, Romboutsia, Clostridium sensu stricto, and Leucobacter in ES, while BS showed greater levels of genera Faecalibacterium and Roseburia. The self-evaluation showed that at least one-half of the tunnel workers experienced one or more symptoms of mental distress (inattention, sleeplessness, loss of appetite, headache or dizziness, irritability) after working in the underground tunnel environment.

Conclusions: Collectively, the underground tunnel environment led to alterations in the intestinal microbiome, which might be relevant to symptoms of mental distress in underground-tunnel workers.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12866-020-02056-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7788853PMC
January 2021

A Motorcyclist-Injury Severity Analysis: A Comparison of Single-, Two-, and Multi-Vehicle Crashes Using Latent Class Ordered Probit Model.

Accid Anal Prev 2021 Mar 29;151:105953. Epub 2020 Dec 29.

The Key Laboratory of Road and Traffic Engineering, Ministry of Education, Tongji University, Shanghai, China.

Motorcycle crashes increasingly become a high proportion of the overall motorized vehicle fatalities. However, limited research has been conducted to compare the injury severity of single-, two- and multi-vehicle crashes involving a motorcycle. This study aims to investigate the effects of rider characteristics, road conditions, pre-crash situations, and crash features on motorcycle severities with respect to different numbers of vehicles involved. The crash data used was obtained through a comprehensive Motorcycle Crash Causation Study (MCCS) by the Federal Highway Administration. An anatomic injury severity indicator, the New Injury Severity Score (NISS), is utilized to calculate a total score as the sum of squared the abbreviated injury scale scores of each of the rider's three most severe injuries. A hybrid approach integrating Latent Class Clustering (LCC) and Ordered Probit (OP) models was used to uncover the unobserved heterogeneity and to explore the major factors which significantly affect the injury severities resulting from single-, two- and multi-vehicle crashes involving a motorcycle. The results show that the significant differences in severity exist between different numbers of vehicles involved. More importantly, they also indicate dividing motorcycle crashes into homogeneous classes before modelling helps to discover insightful information. Pre-speed of the motorcycle is found to be a main factor associated with serious and critical injuries in most types of crashes. Findings of the study provide specific and insightful countermeasures targeting at the contributing factors of motorcycle crashes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.aap.2020.105953DOI Listing
March 2021

A novel polyethersulfone/modified activated carbon fiber composite membrane: potential for removal micropollutants from water under the electric field.

Water Sci Technol 2020 Dec;82(11):2234-2249

Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, College of Environment, Hohai University, Nanjing 210098, China E-mail:

This study aimed to develop a novel composite membrane based on polyethersulfone (PES) and modified activated carbon fibers (ACFs) to remove of sulfamethoxazole (SMZ) from water. The modification of ACFs was conducted by using acid, Fe, and Mn and was confirmed by Fourier transform infrared spectroscopy (FT-IR), energy dispersive X-ray spectroscopy (EDS), and water contact angle measurement. Later on, the composite membranes were prepared using PES (9 wt%), N-N-dimethylacetamide (DMAc) (75 wt%), polyethylene pyrrolidone (PVP) (5 wt%), anhydrous lithium chloride (LiCl) (1 wt%), and various types of modified ACFs (0.8 wt%) as additives. It was found that the contact angle of the membrane decreased by more than 20°, and the zeta potential decreased by more than 10 mV. ACF modified by Fe was used as an admixture, membrane obtained the high comprehensive performance. Especially bovine serum albumin (BSA) rejection rate and flux recovery ratio (FRR) reached 98.8% and 98.4%, respectively. And the removal rates of SMZ increased by 24.6% under the electric field. The degradation products were detected by high-performance liquid chromatography/mass spectrometry (HPLC/MS). Based on this result, the possible degradation pathways of SMZ are proposed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2166/wst.2020.488DOI Listing
December 2020

Aptamer-Peptide Conjugates as Targeted Chemosensitizers for Breast Cancer Treatment.

ACS Appl Mater Interfaces 2021 Mar 11;13(8):9436-9444. Epub 2020 Dec 11.

Molecular Sciences and Biomedicine Laboratory (MBL), State Key Laboratory for Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, and Aptamer Engineering Center of Hunan Province, Hunan University, Changsha 410082, P. R. China.

High levels of heat shock protein 70 (HSP70) in tumors are commonly associated with poor prognosis, enhanced doxorubicin (DOX)-induced cardiotoxicity, and even drug resistance in DOX-related cancer chemotherapy. Several peptides possess remarkable protein inhibition and chemosensitization effects, which are attributed to their specific targeting ability against HSP70. However, the inherent poor cell penetration capacity considerably restricts the biomedical applications of these peptides. We herein describe the design and development of anti-MUC1 aptamer-peptide conjugates (ApPCs) as targeted chemosensitizers to overcome the above-mentioned issues. Moreover, DOX could be loaded on the ApPC to deliver the DOX-enclosed agent ApPC-DOX, which simultaneously acts as a targeted chemosensitizer and anticancer agent for combating drug resistance in breast cancer therapy. This innovative, engineered biocompatible conjugate not only enhances the sensitivity of DOX-resistant cells but also alleviates cardiotoxicity of DOX , highlighting the success of this targeted chemosensitizer strategy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.0c18282DOI Listing
March 2021

A minireview on multiparameter-activated nanodevices for cancer imaging and therapy.

Nanoscale 2020 Nov;12(42):21571-21582

Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, and Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, Hunan 410082, People's Republic of China.

Tumor microenvironment (TME)-responsive nanodevices are essential tools for cancer imaging and therapy. Exploiting the advantages of molecular engineering, nanodevices are emerging for biomedical applications. In order to reach targeted cancer areas, activated nanodevices first respond to the TME and then serve as an actuator for sensing, imaging and therapy. Most nanodevices depend on a single parameter as an input for their downstream activation, potentially leading to inaccurate diagnostic results and poor therapeutic outcomes. However, in the TME, some biomarkers are cross-linked, and such correlated biomarkers are potentially useful for cancer imaging and theranostic applications. Based on this phenomenon, researchers have developed approaches for the construction of multiparameter-activated nanodevices (MANs) to improve accuracy. This minireview summarizes the recent advances in the development of MANs for cancer imaging including fluorescence imaging, photoacoustic (PA) imaging, magnetic resonance imaging (MRI) and computed tomography (CT) imaging, as well as cancer therapy including radiotherapy, chemotherapy, photoinduced therapy and immunotherapy. We highlight different approaches for improving the specificity and precision of cancer imaging and therapy. In the future, MANs will show promise for clinical work in multimodal diagnosis and therapeutics.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0nr04080kDOI Listing
November 2020

RNA editing in cancer impacts mRNA abundance in immune response pathways.

Genome Biol 2020 10 26;21(1):268. Epub 2020 Oct 26.

Bioinformatics Interdepartmental Program, UCLA, Los Angeles, CA, USA.

Background: RNA editing generates modifications to the RNA sequences, thereby increasing protein diversity and shaping various layers of gene regulation. Recent studies have revealed global shifts in editing levels across many cancer types, as well as a few specific mechanisms implicating individual sites in tumorigenesis or metastasis. However, most tumor-associated sites, predominantly in noncoding regions, have unknown functional relevance.

Results: Here, we carry out integrative analysis of RNA editing profiles between epithelial and mesenchymal tumors, since epithelial-mesenchymal transition is a key paradigm for metastasis. We identify distinct editing patterns between epithelial and mesenchymal tumors in seven cancer types using TCGA data, an observation further supported by single-cell RNA sequencing data and ADAR perturbation experiments in cell culture. Through computational analyses and experimental validations, we show that differential editing sites between epithelial and mesenchymal phenotypes function by regulating mRNA abundance of their respective genes. Our analysis of RNA-binding proteins reveals ILF3 as a potential regulator of this process, supported by experimental validations. Consistent with the known roles of ILF3 in immune response, epithelial-mesenchymal differential editing sites are enriched in genes involved in immune and viral processes. The strongest target of editing-dependent ILF3 regulation is the transcript encoding PKR, a crucial player in immune and viral response.

Conclusions: Our study reports widespread differences in RNA editing between epithelial and mesenchymal tumors and a novel mechanism of editing-dependent regulation of mRNA abundance. It reveals the broad impact of RNA editing in cancer and its relevance to cancer-related immune pathways.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13059-020-02171-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7586670PMC
October 2020

Yangonin inhibits ethanol-induced hepatocyte senescence via miR-194/FXR axis.

Eur J Pharmacol 2021 Jan 15;890:173653. Epub 2020 Oct 15.

Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, Dalian, 116044, China. Electronic address:

Chronic alcohol assumption has been recognized as a major cause of alcoholic liver disease (ALD), which ranges from alcoholic steatohepatitis to fibrosis and hepatocellular carcinoma. Alcoholic liver disease has become the leading cause of liver-related health problem in the world. Herewith, effective therapeutic strategy for alcoholic liver disease is necessary. Yangonin (Yan), a bioactive compound extract from Kava, has been reported to exert hepatoprotective effects via Farnesoid X receptor (FXR) activation. The present study aims to investigate whether Yan ameliorated the ethanol-stimulated liver injury and further to elucidate the mechanisms in vivo and in vitro. Yan improved cell viabilities via cell count kit-8 (CCK-8) methods and obviously reduced aspartate aminotransferase (AST), alanine aminotransferase (ALT), total cholesterol (TC) and total triglyceride (TG) levels. We detected miR-194 levels in ethanol-induced LO cells and male C57BL/6 mice by quantitative real-time PCR. Also, the effects of miR-194 on modulating cellular senescence via targeting FXR were further verified. The cellular senescence markers p16, p21, telomerase activity and senescence-related β-galactosidase (SA-β-gal) were evaluated by quantitative real-time PCR and Western blot. Also, LO cells or liver tissues were stained with special primary antibodies and 4',6'-Diamidino-2-phenylindole (DAPI). The cell cycle was detected by flow cytometry. We observed that Yan significantly inhibited ethanol-induced cellular senescence via FXR activation (P < 0.05). Our results demonstrate that Yan significantly reduced the cellular markers p16, p21 and Hmga1 expression and inhibited the cell cycle arrest (P < 0.05). MiR-194 was upregulated in the alcoholic liver disease, which was significantly suppressed by Yan (P < 0.05). Moreover, miR-194 mimic inhibited FXR expression in vitro. In summary, these aggregated data demonstrate that Yan alleviates chronic ethanol-induced liver injury through inhibition of cellular senescence via regulating miR-194/FXR axis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ejphar.2020.173653DOI Listing
January 2021
-->