Publications by authors named "Ting Feng"

192 Publications

Detection of collagen by multi-wavelength photoacoustic analysis as a biomarker for bone health assessment.

Photoacoustics 2021 Dec 26;24:100296. Epub 2021 Aug 26.

Institute of Acoustics, School of Physics Science and Engineering, Tongji University, Shanghai 200092, China.

Collagen is an important biomarker of osteoporosis progression. Noninvasive, multispectral, photoacoustic (PA) techniques use pulsed laser light to induce PA signals to facilitate the visualization of chemical components that are strongly related to tissue health. In this study, the feasibility of multi-wavelength PA (MWPA) measurement of the collagen in bone, using the wavelength range of 1300-1800 nm, was investigated. First, the feasibility of this approach for detecting the collagen content of bone was demonstrated by means of numerical simulation. Then, ex vivo experiments were conducted on both animal and human bone specimens with different bone densities using the MWPA method. The relative collagen content was extracted and compared with the results of micro-computed tomography (micro-CT) and histology. The results showed that the "relative collagen content" parameter obtained using the MWPA approach correlated well with the bone volume ratio obtained from micro-CT images and histological analysis results. This study highlights the potential of the proposed PA technique for determining the collagen content of bones as a biomarker for bone health assessment.
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http://dx.doi.org/10.1016/j.pacs.2021.100296DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8426564PMC
December 2021

Cross-cultural adaptation and validation of the strategic learning assessment map for Chinese nursing organisation: A cross-sectional study.

Nurse Educ Pract 2021 Aug 25;56:103185. Epub 2021 Aug 25.

School of Nursing, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. Electronic address:

Aim: To translate the Strategic Learning Assessment Map into Chinese and validate it in Chinese nursing organisations.

Background: Nursing is the largest occupational organisation in the health sector and its adaptation and innovation are important for the realisation of sustainable development goals. Organisational learning is critical in cultivating the adaptive and innovative abilities of organisations, but there is limited research on its measurement. Although the Strategic Learning Assessment Map is a widely acknowledged organisational measurement instrument, it has not yet been adapted and validated in China.

Design: A cross-sectional study design was used.

Methods: The Chinese version of the Strategic Learning Assessment Map was generated through forward-backward translation and was tested with a convenience sample of 2745 nurses from 7 administrative regions of mainland China. The internal consistency, content validity, structural validity and theoretical framework were examined.

Results: Results validated the theoretical framework and showed excellent content validity, convergent validity and fitness of the measurement model; only discriminant validity was not satisfactory. Cronbach's α values for the overall scale and its subscales ranged from 0.97 to 0.99.

Conclusions: The Chinese version of the Strategic Learning Assessment Map is a reliable organisational learning instrument for Chinese nursing organisations.
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http://dx.doi.org/10.1016/j.nepr.2021.103185DOI Listing
August 2021

Solid-state self-template synthesis of Ta-doped LiZnTiO spheres for efficient and durable lithium storage.

iScience 2021 Sep 18;24(9):102991. Epub 2021 Aug 18.

MOE Key Laboratory of Resources and Environmental Systems Optimization, College of Environmental Science and Engineering, North China Electric Power University, Beijing 102206, China.

Ta-doped LiZnTiO (LZTO) spheres (LiZnTi Ta O; where is the synthetic chemical input,  = 0, 0.03, 0.05, 0.07) are synthesized via solid-state reaction using mesoporous TiO spheres as the self-template. The majority of Ta ions are uniformly doped into crystal lattices of LZTO through the Ti↔Ta substitution, and the rest forms the piezoelectric LiTaO secondary phase on the surface, as confirmed by X-ray diffraction refinement, Raman spectroscopy, density functional theory, and electron microscopy. Electrochemical impedance spectroscopy demonstrates that the Ta doping creates rapid electronic transportation channels for high Li ion diffusion kinetics; however, the LiTaO surface coating is beneficial to improve the electronic conductivity. At the optimal  = 0.05, LiZnTi Ta O spheres exhibit a reversible capacity of 90.2 mAhg after 2000 cycles with a high coulombic efficiency of ≈100% at 5.0 A/g, thus enabling a promising anode material for lithium-ion batteries with high power and energy densities.
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http://dx.doi.org/10.1016/j.isci.2021.102991DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8405915PMC
September 2021

Changes of dendritic cell and natural killer cell on the cord blood with idiopathic fetal growth restriction.

J Matern Fetal Neonatal Med 2021 Aug 11:1-6. Epub 2021 Aug 11.

Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu, PR China.

Objective: To investigate the characteristics of dendritic cells (DC) and natural killer cells (NK) in umbilical cord blood of pregnant patients diagnosed with idiopathic fetal growth restriction (IFGR).

Methods: A prospective study cohort of IFGR patients was established who were in the third trimester (28-36 weeks), with a healthy, pregnant woman cohort selected as controls. Umbilical cord blood was collected.

Results: The study included 50 pregnant women in the IFGR group and 50 pregnant women in the healthy, control group. The incidence of SGA in the IFGR group was 52.0%, and the incidence of preterm birth was 18.0%. The incidence of neonatal complications in neonates with live birth in the IFGR group was 12.0%. The birth weight, body length and placental weight of the newborns in the IFGR group were significantly lower than those in the control group ( < .05). Flow cytometry revealed no significant difference in the proportion or maturity of DC in umbilical cord blood between IFGR group and control group ( > .05). The proportion of NK cells in umbilical cord blood of IFGR group was significantly higher than that of normal control group. The proportion of CD56dimCD16+ NK cells was also significantly higher than that of the normal control group ( < .05), but the expression of NK cell surface killing activator receptor NKG2D and inhibitory receptor NKG2A was not statistically significant ( > .05).

Conclusion: The number and proportion of DC cells in cord blood may not be the key factors affecting the outcomes observed during FGR pregnancies. However, we found the proportion of NK cells in cord blood to be significantly increased, as well as the ratio of CD56dimCD16 + NK to CD56highCD16-NK to be imbalanced, which may be one of the pathogenesis of the pathological pregnancy leading to IFGR.
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http://dx.doi.org/10.1080/14767058.2021.1951214DOI Listing
August 2021

Activated invariant natural killer T cells infiltrate aortic tissue as key participants in abdominal aortic aneurysm pathology.

Immunology 2021 Aug 11. Epub 2021 Aug 11.

Vascular Surgery of West China Hospital, Sichuan University, Chengdu, China.

Adaptive immunity and innate immunity have been implicated in the pathogenesis of abdominal aortic aneurysm (AAA), and damage and remodelling in the tunica media are a focus of the aneurysm development. Thus, identification of key immune cells or molecules that might be targets for the treatment of AAA is critical. We characterized the innate immune cells in human AAA tissue specimens by flow cytometry and found that apart from other lymphocytes, many invariant natural killer T (iNKT) cells marked as CD3 and Va24Ja18 had invaded the aortic tissues and were numerous, especially in the tunica media. These infiltrating iNKT cells have a high expression of CD69, indicating a highly active function. We were interested in whether iNKT cells could be the drivers of media damage in AAA. To answer this question, we used an AAA mouse model induced by angiotensin II (Ang II) infusion, which can reproduce the inflammatory response of AAA in mouse, which was confirmed by RNAseq. The results showed that the incidence of AAA was significantly higher after administration of α-galactosylceramide (α-GalCer), a synthetic glycolipid that activates iNKT cells via CD1d, compared with the Ang II-induced AAA alone (61·54% vs 31·82%) in mice. Histopathological and immunofluorescent staining results showed significantly more severe inflammatory infiltration and pathological lesions in the Ang II+α-GalCer treatment group. These results are highly suggestive that activated iNKT cells greatly contribute to AAA development and that the control of the activation state in iNKT cells may represent an important therapeutic strategy for AAA.
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http://dx.doi.org/10.1111/imm.13401DOI Listing
August 2021

Machine learning model for early prediction of acute kidney injury (AKI) in pediatric critical care.

Crit Care 2021 08 10;25(1):288. Epub 2021 Aug 10.

Department of Information Technology, Phoenix Children's Hospital, Phoenix, AZ, USA.

Background: Acute kidney injury (AKI) in pediatric critical care patients is diagnosed using elevated serum creatinine, which occurs only after kidney impairment. There are no treatments other than supportive care for AKI once it has developed, so it is important to identify patients at risk to prevent injury. This study develops a machine learning model to learn pre-disease patterns of physiological measurements and predict pediatric AKI up to 48 h earlier than the currently established diagnostic guidelines.

Methods: EHR data from 16,863 pediatric critical care patients between 1 month to 21 years of age from three independent institutions were used to develop a single machine learning model for early prediction of creatinine-based AKI using intelligently engineered predictors, such as creatinine rate of change, to automatically assess real-time AKI risk. The primary outcome is prediction of moderate to severe AKI (Stage 2/3), and secondary outcomes are prediction of any AKI (Stage 1/2/3) and requirement of renal replacement therapy (RRT). Predictions generate alerts allowing fast assessment and reduction of AKI risk, such as: "patient has 90% risk of developing AKI in the next 48 h" along with contextual information and suggested response such as "patient on aminoglycosides, suggest check level and review dose and indication".

Results: The model was successful in predicting Stage 2/3 AKI prior to detection by conventional criteria with a median lead-time of 30 h at AUROC of 0.89. The model predicted 70% of subsequent RRT episodes, 58% of Stage 2/3 episodes, and 41% of any AKI episodes. The ratio of false to true alerts of any AKI episodes was approximately one-to-one (PPV 47%). Among patients predicted, 79% received potentially nephrotoxic medication after being identified by the model but before development of AKI.

Conclusions: As the first multi-center validated AKI prediction model for all pediatric critical care patients, the machine learning model described in this study accurately predicts moderate to severe AKI up to 48 h in advance of AKI onset. The model may improve outcome of pediatric AKI by providing early alerting and actionable feedback, potentially preventing or reducing AKI by implementing early measures such as medication adjustment.
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http://dx.doi.org/10.1186/s13054-021-03724-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8353807PMC
August 2021

Pollution risk estimation of the Cu element in atmospheric sedimentation samples by laser induced breakdown spectroscopy (LIBS) combined with random forest (RF).

Anal Methods 2021 08 13;13(30):3424-3432. Epub 2021 Jul 13.

Key Laboratory of Synthetic and Natural Functional Molecule of the Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi'an, 710127, China.

Laser-induced breakdown spectroscopy (LIBS) combined with the random forest (RF) algorithm was proposed to predict three pollution indexes (geo-accumulation index, enrichment factor, and potential ecological risk index) of the Cu element in atmospheric sedimentation samples to evaluate the pollution risk. To begin with, the LIBS spectra of 15 atmospheric sedimentation samples from different locations were collected and the copper element was identified using the National Institute of Standards and Technology (NIST) database. Then, the influence of different spectral pretreatment methods (MSC, WT and D) on the predictive performance of the RF was discussed according to the calibration set with the determination coefficient (R) and mean relative error (MREC) as evaluation indexes. Next, in order to obtain a better RF calibration model, a variable importance (VI) measurement was applied to select input variables from LIBS spectral data based on the optimal spectral pretreatment method, and the optimal variable importance threshold was selected as the input variable to establish the RF calibration model. Finally, the predictive performance of the optimal RF calibration model was verified using the prediction set with the determination coefficient (R) and the mean relative error (MREP). The results show that R of the geo-accumulation index, enrichment factor and potential ecological risk index is up to 0.9971, 0.9919 and 0.9290, respectively, and MREP of the three indexes is 0.0234, 0.1173 and 0.0810, respectively; the average relative standard deviation (RSD) of the prediction set for the three indexes is 2.16%, 5.78% and 0.71%, respectively. Furthermore, it can be inferred that Cu was at levels corresponding to serious pollution primarily because of anthropogenic activities based on the predictive I, E and RI values. Therefore, LIBS combined with the RF algorithm is a promising means which can achieve fast and simple estimation of the pollution risk degree of Cu in atmospheric sedimentation samples without complicated sample preparation to provide a basis for pollution prevention and control measures.
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http://dx.doi.org/10.1039/d1ay00879jDOI Listing
August 2021

Carbonaceous aerosols in urban Chongqing, China: Seasonal variation, source apportionment, and long-range transport.

Chemosphere 2021 Jul 8;285:131462. Epub 2021 Jul 8.

Department of Environmental Science and Engineering, Fudan University, Shanghai, 200433, China.

Seventy-seven PM2.5 samples were collected at an urban site (Chongqing University Campus A) in October 2015 (autumn), December 2015 (winter), March 2016 (spring), and August 2016 (summer). These samples were analysed for organic carbon (OC), elemental carbon (EC), and their associated char, soot, 16 PAHs, and 28 n-alkanes to trace sources, and atmospheric transport pathways. The annual average of OC, EC, char, soot, ΣPAHs, and Σn-alkanes were 20.75 μg/m, 6.18 μg/m, 5.43 μg/m, 0.75 μg/m, 38.29 ng/m, and 328.69 ng/m, respectively. OC, ΣPAHs, and Σn-alkane concentrations were highest in winter and lowest in summer. EC, char, and soot concentrations were highest in autumn and lowest in winter. Source apportionment via positive matrix factorization (PMF) indicated that coal/biomass combustion-natural gas emissions (23.8%) and motor vehicle exhaust (20.2%) were the two major sources, followed by diesel and petroleum residue (21.1%), natural biogenic sources (17.7%), and evaporative/petrogenic sources (17.2%). The highest source contributor in autumn and winter was evaporative/petrogenic sources (30.6%) and natural biogenic sources (34.5%), respectively, whereas diesel engine emission contributed the most in spring and summer (32.1% and 38.0%, respectively). Potential source contribution function (PSCF) analysis identified southeastern Sichuan and northwestern Chongqing as the major potential sources of these pollutants. These datasets provide critical information for policymakers to establish abatement strategies for the reduction of carbonaceous pollutant emissions and improve air quality in Chongqing and other similar urban centres across China.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131462DOI Listing
July 2021

Amorphous Fe(OH) Passivating CeO Nanorods: A Noble-Metal-Free Photocatalyst for Water Oxidation.

ChemSusChem 2021 Aug 22;14(16):3382-3390. Epub 2021 Jul 22.

College of Materials Science and Engineering, Ocean University of China, Songling road No. 238, QingDao city, P. R. China.

Noble-metal-free composites with good photocatalytic property are of great interest. Here, CeO nanorods composites loaded with amorphous Fe(OH) cocatalyst were designed and prepared via a secondary water bath at 100 °C. The as-synthesized CeO /amorphous Fe(OH) composites exhibited superior light photocatalytic activities compared to pure CeO , especially the sample with a loading time of 60 min. The photocatalytic oxygen generation rate could reach to 357.2 μmol h  g , and the average apparent quantum yield (AQY) was 24.67 %, which was a 5.5-fold increase compared to the CeO sample. The improvement of photocatalytic performance could be ascribed to three main reasons: First, loading the amorphous Fe(OH) enlarged the specific surface area and passivated the surface of the pristine CeO . Second, the amorphous Fe(OH) ,which acted as a cocatalyst, provided many active sites, and reduced the reaction activation energy. Thirdly, the maximum interface with intimate contact between CeO and amorphous Fe(OH) cocatalyst accelerated the photogenerated charge separation efficiency and thus improved the photocatalytic performance of CeO in photocatalytic water oxidation.
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http://dx.doi.org/10.1002/cssc.202101061DOI Listing
August 2021

Application of Pulsed Rhythmic Drug Administration to Ovulation Induction Therapy in PCOS Patients with Clomiphene-Resistance: a Retrospective Research.

Reprod Sci 2021 Jun 3. Epub 2021 Jun 3.

Reproductive Medical Center of the Second Affiliated Hospital of Nanjing Medical University, Nanjing, 210011, China.

There is currently a dispute over the choice of ovulation induction treatment for infertile women with polycystic ovary syndrome (PCOS). The objective of this study is to compare the therapeutic effect of pulsed rhythmic administration protocol (PRAP) with conventional letrozole + human menopausal gonadotropin (HMG) in patients with clomiphene-resistance polycystic ovary syndrome (PCOS). A retrospective analysis of 821 intrauterine insemination (IUI) cycles between January 2015 and January 2020 was performed. Of these, 483 cycles were treated with a pulsed rhythmic administration protocol (PRAP), and 338 cycles were treated with conventional letrozole + HMG protocol (LHP). The therapeutic effect of the two protocols has been compared. The pregnancy rate was 18.07% in the LHP and 27.07% in the PRAP. The ongoing pregnancy rate in LHP was 14.46% and in PRAP was 22.73%. The research suggests that PRAP is more effective than LHP and could be an adequate ovulation induction strategy for the IUI cycle of patients with clomiphene-resistance PCOS.
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http://dx.doi.org/10.1007/s43032-021-00639-7DOI Listing
June 2021

Distributed transverse-force sensing along a single-mode fiber using polarization-analyzing OFDR: erratum.

Opt Express 2021 May;29(10):14355

We correct an inaccurate statement on the birefringence measurement using the PA-OFDR in our previous publication [Opt. Express28, 31253 (2020)10.1364/OE.405682].
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http://dx.doi.org/10.1364/OE.427536DOI Listing
May 2021

Widely tunable single-/dual-wavelength fiber lasers with ultra-narrow linewidth and high OSNR using high quality passive subring cavity and novel tuning method: erratum.

Opt Express 2021 May;29(10):14354

We provide a corrected equation of our previous publication [Opt. Express24, 19760 (2016)10.1364/OE.24.019760].
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http://dx.doi.org/10.1364/OE.427535DOI Listing
May 2021

Experience-Dependent Inhibitory Plasticity Is Mediated by CCK+ Basket Cells in the Developing Dentate Gyrus.

J Neurosci 2021 May 27;41(21):4607-4619. Epub 2021 Apr 27.

Department of Biology, University of Nevada, Reno, Nevada 89557

Early postnatal experience shapes both inhibitory and excitatory networks in the hippocampus. However, the underlying circuit plasticity is unclear. Using an enriched environment (EE) paradigm during the preweaning period in mice of either sex, we assessed the circuit plasticity of inhibitory cell types in the hippocampus. We found that cholecystokinin (CCK)-expressing basket cells strongly increased somatic inhibition on the excitatory granular cells (GCs) following EE, whereas another pivotal inhibitory cell type, parvalbumin (PV)-expressing cells, did not show changes. Using electrophysiological analysis and the use of cannabinoid receptor 1 (CB1R) agonist WIN 55 212-2, we demonstrate that the change in somatic inhibition from CCK+ neurons increases CB1R-mediated inhibition in the circuit. By inhibiting activity of the entorhinal cortex (EC) using a chemogenetic approach, we further demonstrate that the activity of the projections from the EC mediates the developmental assembly of CCK+ basket cell network. Altogether, our study places the experience-dependent remodeling of CCK+ basket cell innervation as a central process to adjust inhibition in the dentate gyrus and shows that cortical inputs to the hippocampus play an instructional role in controlling the refinement of the synaptic connections during the preweaning period. Brain plasticity is triggered by experience during postnatal brain development and shapes the maturing neural circuits. In humans, altered experience-dependent plasticity can have long-lasting detrimental effects on circuit function and lead to psychiatric disorders. Yet, the cellular mechanisms governing how early experience fine-tunes the maturing synaptic network is not fully understood. Here, taking advantage of an enrichment-housing paradigm, we unravel a new plasticity mechanism involved in the maintenance of the inhibitory to excitatory balance in the hippocampus. Our findings demonstrate that cortical activity instructs the assembly of the CCK+ basket cell network. Considering the importance of this specific cell type for learning and memory, experience-dependent remodeling of CCK+ cells may be a critical determinant for establishing appropriate neural networks.
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http://dx.doi.org/10.1523/JNEUROSCI.1207-20.2021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260240PMC
May 2021

Stable, precisely controlled, and switchable thulium-doped fiber laser based on cascaded mode interference filters.

Opt Express 2021 Mar;29(7):9786-9796

This research experimentally demonstrates a switchable, single-wavelength, thulium-doped fiber laser based on the cascading of a multimode-single-mode-multimode (MSM) fiber filter and a two-mode fiber (TMF) filter. When the MSM fiber filter suffers from bending, the blue-shift of the output spectrum can be obtained. A switchable lasing wavelength output is realized by bending the MSM fiber filter to cover different channels of the TMF filter. The output wavelength can be switched from 1982.54 to 1938.81 nm with an optical signal-to-noise ratio of higher than 40 dB. The wavelength interval of the switchable output is an integral multiple of the wavelength interval of the TMF filter. The stability of the output wavelength was tested within 60 min, and the wavelength shift and output power fluctuation were found to be less than 0.01 nm and 0.31 dB, respectively, which demonstrates a stable output performance.
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http://dx.doi.org/10.1364/OE.419283DOI Listing
March 2021

Wavelet transform-based photoacoustic time-frequency spectral analysis for bone assessment.

Photoacoustics 2021 Jun 10;22:100259. Epub 2021 Mar 10.

Institute of Acoustics, School of Physics Science and Engineering, Tongji University, Shanghai, PR China.

In this study, we investigated the feasibility of using photoacoustic time-frequency spectral analysis (PA-TFSA) for evaluating the bone mineral density (BMD) and bone structure. Simulations and experiments on bone samples with different BMDs and mean trabecular thickness (MTT) were conducted. All photoacoustic signals were processed using the wavelet transform-based PA-TFSA. The power-weighted mean frequency (PWMF) was evaluated to obtain the main frequency component at different times. The , , and of the linearly fitted curve of the PWMF over time were also quantified. The results show that the osteoporotic bone samples with lower BMD and thinner MTT have higher frequency components and lower acoustic frequency attenuation over time, thus higher , , and . The and were found to be sensitive to the BMD; therefore, both parameters could be used to distinguish between osteoporotic and normal bones ( < 0.05).
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http://dx.doi.org/10.1016/j.pacs.2021.100259DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7985564PMC
June 2021

Circadian clock: a regulator of the immunity in cancer.

Cell Commun Signal 2021 Mar 22;19(1):37. Epub 2021 Mar 22.

Department of Internal Medicine, College of Integrated Chinese and Western Medicine of Hunan University of Chinese Medicine, 300 Xueshi Road, Changsha, 410007, Hunan, People's Republic of China.

The circadian clock is an endogenous timekeeper system that controls and optimizes biological processes, which are consistent with a master circadian clock and peripheral clocks and are controlled by various genes. Notably, the disruption of circadian clock genes has been identified to affect a wide range of ailments, including cancers. The cancer-immunity cycle is composed of seven major steps, namely cancer cell antigen release and presentation, priming and activation of effector immunity cells, trafficking, and infiltration of immunity to tumors, and elimination of cancer cells. Existing evidence indicates that the circadian clock functions as a gate that govern many aspects of the cancer-immunity cycle. In this review, we highlight the importance of the circadian clock during tumorigenesis, and discuss the potential role of the circadian clock in the cancer-immunity cycle. A comprehensive understanding of the regulatory function of the circadian clock in the cancer-immunity cycle holds promise in developing new strategies for the treatment of cancer. Video Abstract.
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http://dx.doi.org/10.1186/s12964-021-00721-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7986390PMC
March 2021

Interleukin 35 protects cardiomyocytes following ischemia/reperfusion-induced apoptosis via activation of mitochondrial STAT3.

Acta Biochim Biophys Sin (Shanghai) 2021 Mar;53(4):410-418

Department of Cardiology, State Key Laboratory of Organ Failure Research, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.

Mitochondrial reactive oxygen species (mtROS)-induced apoptosis has been suggested to contribute to myocardial ischemia/reperfusion injury. Interleukin 35 (IL-35), a novel anti-inflammatory cytokine, has been shown to protect the myocardium and inhibit mtROS production. However, its effect on cardiomyocytes upon exposure to hypoxia/reoxygenation (H/R) damage has not yet been elucidated. The present study aimed to investigate the potential protective role and underlying mechanisms of IL-35 in H/R-induced mouse neonatal cardiomyocyte injury. Mouse neonatal cardiomyocytes were challenged to H/R in the presence of IL-35, and we found that IL-35 dose dependently promotes cell viability, diminishes mtROS, maintains mitochondrial membrane potential, and decreases the number of apoptotic cardiomyocytes. Meanwhile, IL-35 remarkably activates mitochondrial STAT3 (mitoSTAT3) signaling, inhibits cytochrome c release, and reduces apoptosis signaling. Furthermore, co-treatment of the cardiomyocytes with the STAT3 inhibitor AG490 abrogates the IL-35-induced cardioprotective effects. Our study identified the protective role of IL-35 in cardiomyocytes following H/R damage and revealed that IL-35 protects cardiomyocytes against mtROS-induced apoptosis through the mitoSTAT3 signaling pathway during H/R.
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http://dx.doi.org/10.1093/abbs/gmab007DOI Listing
March 2021

An optimized segmentation and quantification approach in microvascular imaging for OCTA-based neovascular regression monitoring.

BMC Med Imaging 2021 01 22;21(1):13. Epub 2021 Jan 22.

School of Electronic and Optical Engineering, Nanjing University of Science and Technology, Nanjing, 210094, China.

Background: Quantification of neovascularization changes in terms of neovascular complex (NVC) acquired from the optical coherence tomography angiography (OCTA) imaging is extremely important for diagnosis and treatment monitoring of proliferative diabetic retinopathy (PDR). However, only few vessel extraction methods have so far been reported to quantify neovascular changes in NVC with proliferative diabetic retinopathy PDR based on OCTA images.

Methods: Here we propose an optimized approach to segment blood vessels, which is based on an improved vascular connectivity analysis (VCA) algorithm and combined with morphological characterization and elimination of noise and artifacts. The length and width of vessels are obtained in the quantitative assessment of microvascular network. The feasibility of the proposed method is further studied by a treatment monitoring and statistical analysis process, as we have monitored and statistically analyzed the changes of NVC based on sampled OCTA images of PDR patients (N = 14) after treatment by intravitreal injection of conbercept.

Results: The proposed method has demonstrated better performance in accuracy compared with existing algorithms and can thus be used for PRD treatment monitoring. Following the PDR treatment monitoring study, our data has shown that from the 1st day to 7th day of treatment, the averaged (arithmetic mean) length of NVC has been substantially shortened by 36.8% (P < 0.01), indicating significant effects of treatment. Meanwhile, the averaged (arithmetic mean) width of NVC from the 1st day to 7th day of treatment has been increased by 10.2% (P < 0.05), indicating that most of the narrow neovascularization has been reduced.

Conclusion: The results and analysis have confirmed that the proposed optimization process by the improved VCA method is both effective and feasible to segment and quantify the NVC with lower noise and fewer artifacts. Thus, it can be potentially applied to monitor the fibrovascular regression during the treatment period. Clinical Trial Registration This trial is registered with the Chinese Clinical Trial Registry (Registered 27 December 2017, http://www.chictr.org.cn , registration number ChiCTR-IPR-17014160).
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http://dx.doi.org/10.1186/s12880-021-00546-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7825210PMC
January 2021

A flexible short protocol in women with poor ovarian response over 40 years old.

J Ovarian Res 2021 Jan 5;14(1). Epub 2021 Jan 5.

Reproductive Medical Center of the Second Affiliated Hospital of Nanjing Medical University, 121 Jiangjiayuan, 210011, Nanjing, China.

Background: Ovarian responsiveness to controlled ovarian stimulation is essential for a successful clinical outcome in assisted reproductive technology (ART) cycles. We aimed to find a suitable new ovulation stimulation protocol for poor ovarian response (POR) patients over 40 years old.

Methods: A retrospective analysis of 488 ART cycles was evaluated from January 2015 to June 2019. Comparisons were made between the flexible short protocol (FSP), routine short protocol and mild stimulation protocol.

Results: Compared with the routine short protocol, the FSP delayed the gonadotropin start time and reduced the total gonadotropin dose per stimulation cycle. At the same time, compared with the mild stimulation protocol, the FSP improved oocyte quality and embryo quality and improved embryo implantation potential after transfer. Furthermore, the use of the FSP reduced the probability of premature ovulation, as it inhibited the premature luteinizing hormone (LH) surge to a certain extent.

Conclusions: The FSP yielded better outcomes than other protocols for patients with POR over 40 years old in our study. However, further prospective studies are needed to provide more substantial evidence and to determine whether the FSP can be successful for both patients over 40 years group and younger POR patients.
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http://dx.doi.org/10.1186/s13048-020-00761-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7786950PMC
January 2021

Identification of Transcriptional Variation in Aortic Remodeling Using a Murine Transverse Aortic Constriction (TAC) Model.

Front Cardiovasc Med 2020 12;7:581362. Epub 2020 Nov 12.

Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Arterial remodeling is a major pathological consequence of hypertension, which is recognized as the most common chronic non-communicable disease. However, the detailed mechanism of how arterial remodeling is induced by hypertension has not yet been fully elucidated. Evaluating the transcriptional changes in arterial tissue in response to elevated blood pressure at an early stage may provide new insights and identify novel therapeutic candidates in preventing arterial remodeling. Here, we used the ascending aorta of the transverse aortic constriction (TAC) model to induce arterial remodeling in C57BL/6 male mice. Age-matched mice were subjected to sham surgery as controls. The TAC model was only considered successful if the mice conformed to the criteria (RC/LC blood flow velocity with 5-10-fold change) 1 week after the surgery. Two weeks after surgery, the ascending aorta developed severe remodeling in TAC mice as compared to the sham group. High throughput sequencing was then applied to identify differentially expressed (DE) transcripts. In silicon analysis were then performed to systematically network transcriptional changes. A total of 1,019 mRNAs were significantly changed between TAC and the sham group at the transcriptional level. GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) analysis revealed that stress/stimulus/immune-related biological processes played a crucial role during arterial remodeling. Our data provide a comprehensive understanding of global gene expression changes in the TAC model, which suggests that targeting inflammation and vascular smooth cell transformation are potential therapeutic strategies to interfere with the aortic remodeling at an early stage in the development of hypertension.
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http://dx.doi.org/10.3389/fcvm.2020.581362DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7693635PMC
November 2020

Distributed transverse-force sensing along a single-mode fiber using polarization-analyzing OFDR.

Opt Express 2020 Oct;28(21):31253-31271

We report what we believe to be the first demonstration of a direct distributed transverse-force (TF) sensing along a single-mode fiber (SMF) using a self-built polarization-analyzing optical frequency-domain reflectometry (PA-OFDR). The transverse line-force (TLF) distribution along a SMF can be directly obtained from the absolute measurement of birefringence induced by the TF via photo-elastic effect at different locations along the fiber, without the need of complicated force-to-strain conversion. We show that our system is capable of sensing a weight of merely 0.68 g but yet has a large dynamic range of over 44 dB. In particular, we obtained a maximum detectable TLF of 16.8 N/mm, a minimum detectable TLF of 6.61×10 N/mm, a TLF measurement uncertainty of <2.432%, a TF sensing spatial resolution of 3.7 mm and a TF sensing distance of 103.5 m. We also experimentally investigated the influence of different fiber coatings on the TF sensing and found that the polyimide coating is a better choice due to its high TF measurement sensitivity and response speed, although it induces relatively high residual birefringence in the SMF to limit the minimum detectable TLF. Our work is an important step forward for practical distributed TF sensing and shall prove useful for engineers and scientists to implement the PA-OFDR technology for distributed TF sensing with low cost SMFs.
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http://dx.doi.org/10.1364/OE.405682DOI Listing
October 2020

The Maternal-Fetal Interface in Small-for-Gestational-Age Pregnancies Is Associated With a Reduced Quantity of Human Decidual NK Cells With Weaker Functional Ability.

Front Cell Dev Biol 2020 3;8:633. Epub 2020 Sep 3.

Center for Translational Medicine, Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, China.

Small for gestational age (SGA) refers to a birth weight that is less than the 10th percentile of the mean weight of infants at the same gestational age. This condition is associated with a variety of complications, and a high risk of cardiovascular and cerebrovascular diseases in adulthood. Decidual natural killer (dNK) cells at the maternal-fetal interface have received significant research attention in terms of normal pregnancy or miscarriage; however, data relating to SGA are limited. In this study, we aimed to investigate the characteristics and regulatory role of dNK cells at the maternal-fetal interface in SGA. Using immunofluorescence assays, we found that dNK cells maintained close contact with extra-villous trophoblasts, and the proportion of dNK cells in SGA decreased more than in appropriate for gestational age (AGA). Flow cytometry also showed that there was a significantly lower percentage of dNK cells in SGA (25.01 ± 2.43%) than in AGA (34.25 ± 2.30%) ( = 0.0103). The expression of the inhibitory receptor NKG2A on dNK cells and the secretion levels of both perforin and TGF-β1 from dNK cells were significantly higher in SGA than in AGA, while the cytotoxicity of dNK cells in SGA against K562 cells was attenuated. Compared to AGA, the functional ability of dNK cells in SGA showed significant functional impairment in promoting proliferation, migration, invasion, and tube formation in trophoblast cells or vascular endothelial cells. The abnormal function of dNK cells may affect fetal growth and development, and could therefore participate in the pathogenesis of SGA.
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http://dx.doi.org/10.3389/fcell.2020.00633DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7509437PMC
September 2020

Nitrate-assisted biodegradation of polycyclic aromatic hydrocarbons (PAHs) in the water-level-fluctuation zone of the three Gorges Reservoir, China: Insights from in situ microbial interaction analyses and a microcosmic experiment.

Environ Pollut 2021 Jan 21;268(Pt B):115693. Epub 2020 Sep 21.

State Key Laboratory of Coal Mine Disaster Dynamics and Control, Chongqing University, Chongqing, 400044, China; Department of Environmental Science, Chongqing University, Chongqing, 400044, China.

An increase in polycyclic aromatic hydrocarbon (PAH) pollution poses significant challenges to human and ecosystem health in the Three Gorges Reservoir (TGR) of the Yangtze River. Based on the combination of PAH analysis with qPCR and high-throughput sequencing of bacteria, 32 topsoil samples collected from 16 sites along the TGR were used to investigate the distribution and biodegradation pathways of PAHs in the water-level-fluctuation zone (WLFZ). The results indicated that the concentrations of PAHs were 43.8-228.2 and 30.8-206.3 ng/g soil (dry weight) under the high- and low-water-level (HWL and LWL) conditions, respectively. The PAH concentration in urban areas was higher than that in rural areas. Under both the HWL and LWL conditions, the abundance of the bamA gene, a biomarker of anaerobic PAH biodegradation, was significantly higher than that of the ring-hydroxylating-dioxygenase (RHD) gene, a biomarker of aerobic PAH biodegradation. The abundance of the bamA gene was significantly positively correlated with PAHs (R = 0.8), and the biodegradation percentage of PAHs incubated anaerobically was greater than that in the aerobically incubated microcosm experiments. These data implicated a key role of the anaerobic pathway in PAH biodegradation. Co-occurrence network analysis suggested that anaerobic Anaerolineaceae, Dechloromonas, Bacteroidetes_vadin HA17 and Geobacter were key participants in the biodegradation of PAHs. The diversity analysis of functional bacteria based on the bamA gene and microcosm experiments further demonstrated that nitrate was the primary electron acceptor for PAH biodegradation. These findings provide a new perspective on the mechanism of PAH biodegradation in the TGR and knowledge that can be used to develop strategies for environmental management.
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http://dx.doi.org/10.1016/j.envpol.2020.115693DOI Listing
January 2021

The relationship between semen factors and unexplained recurrent spontaneous abortion.

Clin Chim Acta 2020 Nov 20;510:605-612. Epub 2020 Aug 20.

Reproductive Medical Center of Second Affiliated Hospital of Nanjing Medical University, Nanjing 210011, China. Electronic address:

Background: The male factor may contribute to unexplained recurrent spontaneous abortion (URSA). The relationship between semen factors and URSA is largely unknown. The analysis of metabolomics which is broadly acknowledged as the omics closest to the phenotype is suitable for providing assistance in the semen parameters analysis.

Methods: We conducted a study including couples with URSA and controls which was next combined with a meta-analysis, and finally the study included 2352 subjects on semen parameters and URSA. Metabolomics analysis was conducted to detect semen metabolic factors related to URSA in total of 106 samples including seminal plasma and sperm cells.

Results: The URSA group had significantly lower total sperm count, sperm concentration, motility and normal morphology percentage. Meta-analysis next showed consistent findings. Metabolomics revealed that 4 metabolites and one pathway and 8 metabolites and one pathway were significantly associated with URSA in sperm and seminal plasma, respectively. The combination of ascorbic acid and guanine in seminal plasma and hexadecanedioic acid and pyroglutamic acid in sperm showed ability for URSA prediction.

Conclusion: We provided novel insights into semen indices in relation to URSA. Lower sperm number and quality might increase the risk of URSA, and oxidative stress and hormone metabolism in sperm as well as nucleic acid synthesis and oxidative stress in seminal plasma were related to URSA.
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http://dx.doi.org/10.1016/j.cca.2020.08.022DOI Listing
November 2020

Human follicular fluid shows diverse metabolic profiles at different follicle developmental stages.

Reprod Biol Endocrinol 2020 Jul 23;18(1):74. Epub 2020 Jul 23.

Reproductive Medicine Center of the Second Affiliated Hospital of Nanjing Medical University, Nanjing, 210011, China.

Background: To investigate the metabolic profiles in the follicular fluid (FF) samples from patients undergoing in vitro fertilization (IVF) and to analyze the correlations with follicular development.

Methods: The FF samples were obtained from participants (N = 26) who were receiving IVF under the gonadotropin-releasing hormone agonist (GnRH-a) long protocol stimulation and were collected separately from small (8-13 mm) and large (17-22 mm) follicles at the time of oocyte retrieval. Metabolomic analysis of the FF samples was performed using liquid chromatography-tandem mass spectrometry (LC-MS/MS).

Results: The results demonstrated that the size of the follicle influences the metabolic signature of the FF according to the profile and differential metabolites. Dehydroepiandrosterone (DHEA), which is enriched in steroid hormone biosynthesis, correlated negatively with the oocyte maturation rate and the high-quality embryo rate, and thus could be used to estimate the predictive diagnostic potential of follicular development.

Conclusion: The FF has different metabolic characteristics in different stages of follicular development. Exploring meaningful metabolites could predict follicular development, and modifications of these metabolites could influence follicular development.
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http://dx.doi.org/10.1186/s12958-020-00631-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7376676PMC
July 2020

Rapid preparation of ultra-fine and well-dispersed SnO nanoparticles via a double hydrolysis reaction for lithium storage.

Nanoscale 2020 Aug 16;12(29):15697-15705. Epub 2020 Jul 16.

School of Physics and Engineering, Henan University of Science and Technology, Luoyang 471023, China.

An efficient and rapid method is reported for preparing ultra-fine and well-dispersed SnO nanoparticles in a large scale. A simple double hydrolysis reaction between SnO and Fe ions was masterly used to form a stable colloid system, in which colloidal particles of HSnO with negative charges and Fe(OH) with positive charges electrostatically interact with each other and form honeycomb-like "core-shell" units. Through the hydrothermal reaction, the units are easily transformed into [email protected](OH) structures. Ultra-fine and well-dispersed SnO particles with less than 6 nm diameter were finally obtained with a high yield by further etching using hydrochloric acid. When used as anode materials for lithium ion batteries, the ultra-fine SnO particles can be easily dispersed into the carbon networks originating from the carbon source of glucose during the hydrothermal reaction. Electrochemical tests confirmed that these ultra-fine SnO/C materials were endowed with excellent cyclic stability and C-rate performance. Even at a 1.56 A g (2C) high current density, the reversible capacity could be maintained at 710 mA h g after 100 cycles owing to the ultra-fine particle size of SnO and the rich carbon networks.
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http://dx.doi.org/10.1039/d0nr02219eDOI Listing
August 2020

PM fiber based sensing tapes with automated 45° birefringence axis alignment for distributed force/pressure sensing.

Opt Express 2020 Jun;28(13):18829-18842

Polarization maintaining (PM) fibers can be used for distributed force/pressure sensing in which the birefringence axis of the PM fiber should preferably be oriented 45° from the direction of the force/pressure for the maximum sensitivity. However, it is a challenge to achieve such 45° axis orientation for a long length of PM fiber in practice. In this paper, we report the development of what we believe the first equipment and process for making PM fiber based sensing tapes, capable of automatically adjusting the fiber axis orientation 45° with respect to the tape surface. In particular, we develop a machine vision system with the ability of continuously determining fiber axis orientation in real time as the fiber passes by and feeding back the orientation information to a fiber rotation apparatus to automatically adjust its orientation before fixing the fiber on a transparent PET tape with UV epoxy. We show the results of a successfully fabricated 70-m-long PM fiber sensing tape achieving an axis orientation accuracy of 45 ± 3° throughout the whole length of the tape, which is further validated with a distributed polarization crosstalk analyzer (DPXA). Finally, we demonstrate distributed transversal load sensing with 14 force applying weights randomly distributed along the sensing tape using the DPXA, with a polarization crosstalk measurement uniformity of 0.62 dB (standard deviation) using the same applied weight of 100 grams. The same sensing tape can also be used for pressure sensing with properly designed fixtures.
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http://dx.doi.org/10.1364/OE.391376DOI Listing
June 2020

Surface Roughed and Pt-Rich Bimetallic Electrocatalysts for Hydrogen Evolution Reaction.

Front Chem 2020 4;8:422. Epub 2020 Jun 4.

School of Physics and Engineering, Henan University of Science and Technology, Luoyang, China.

Platinum-based alloys with low cost transition metals have been considered as promising electrocatalysts in the field of sustainable energy conversion and storage. Herein, chloroplatinic acid, cobalt chloride, and carbon nanotubes are used as platinum, cobalt precursors, and carriers, respectively, to prepare rich Pt dealloying PtCo nanoparticles (SD-PtCo/CNT) via co-liquid phase reduction and chemical dealloying methods. The characterization and test results confirm that PtCo alloy nanoparticles are evenly dispersed on carbon nanotubes, further dealloying and resulting in the partial dissolving of cobalt, simultaneously generating a rich Pt layer and roughly active surface. Benefiting from the unique structure, the SD-PtCo/CNT catalyst displays obviously enhanced HER activity in both acidic and alkaline conditions. In 1.0 M KOH, SD-PtCo/CNT exhibits a low overpotential of 78 mV at 10 mA/cm and a small tafel slope (38.28 mV/dec). In 0.5 M HSO, SD-PtCo/CNT still shows the superior performance compared with un-dealloying PtCo/CNT, with an overpotential of 17 mV at 10 mA/cm and corresponding tafel slope of 21.35 mV/dec. The high HER activity of SD-PtCo/CNT can be attributed to the formation of a platinum rich layer and the uniformly dispersed PtCo nanoparticles supported on superior conductive carbon nanotubes, suggesting its great potential for hydrogen generation via water splitting.
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http://dx.doi.org/10.3389/fchem.2020.00422DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7287206PMC
June 2020

Contributions and achievements on schistosomiasis control and elimination in China by NIPD-CTDR.

Adv Parasitol 2020 10;110:1-62. Epub 2020 Jun 10.

National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention; Chinese Center for Tropical Diseases Research, Shanghai, People's Republic of China; WHO Collaborating Centre for Tropical Diseases; National Center for International Research on Tropical Diseases, Ministry of Science and Technology; Key Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai, People's Republic of China; School of Global Health, Chinese Center for Tropical Diseases Research, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China. Electronic address:

Being a zoonotic parasitic disease, schistosomiasis was widely spread in 12 provinces of Southern China in the 1950s, severly harming human health and hindering economic development. The National Institute of Parasitic Diseases at the Chinese Center for Diseases Control and Prevention, and Chinese Center for Tropical Diseases Research (NIPD-CTDR), as the only professional institution focussing on parasitic diseases at the national level, has played an important role in schistosomiasis control in the country. In this article, we look back at the changes of schistosomiasis endemicity and the contribution of NIPD-CTDR to the national schistosomiasis control programme. We review NIPD-CTDR's activities, including field investigations, design of control strategies and measures, development of diagnostics and drugs, surveillance-response of endemic situation, and monitoring & evaluation of the programme. The NIPD-CTDR has mastered the transmission status of schistosomiasis, mapped the snail distribution, and explored strategies and measures suitable for different types of endemic areas in China. With a good understanding of the life cycle of Schistosoma japonicum and transmission patterns of the disease, advanced research carried out in the NIPD-CTDR based on genomics and modern technology has made it possible to explore highly efficient and soft therapeutic drugs and molluscicides, making it possible to develop new diagnostic tools and produce vaccine candidates. In the field, epidemiological studies, updated strategies and targeted intervention measures developed by scientists from the NIPD-CTDR have contributed significantly to the national schistosomiasis control programme. This all adds up to a strong foundation for eliminating schistosomiasis in China in the near future, and recommendations have been put forward how to reach this goal.
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http://dx.doi.org/10.1016/bs.apar.2020.04.002DOI Listing
June 2021

Elimination of long 3'-UTR mRNA isoform by CRISPR-Cas9 gene editing impairs dorsal root ganglion development and hippocampal neuron activation in mice.

RNA 2020 10 10;26(10):1414-1430. Epub 2020 Jun 10.

Department of Biology, University of Nevada, Reno, Nevada 89557, USA.

The majority of mouse and human genes are subject to alternative cleavage and polyadenylation (APA), which most often leads to the expression of two or more alternative length 3' untranslated region (3'-UTR) mRNA isoforms. In neural tissues, there is enhanced expression of APA isoforms with longer 3'-UTRs on a global scale, but the physiological relevance of these alternative 3'-UTR isoforms is poorly understood. ( is a key integrator of calcium signaling that generates short () and long () 3'-UTR mRNA isoforms via APA. We found expression to be largely restricted to neural tissues in mice including the dorsal root ganglion (DRG) and hippocampus, whereas was more broadly expressed. smFISH revealed that both and were subcellularly localized to neural processes of primary hippocampal neurons. In contrast, cultured DRG showed restriction of to soma. To investigate the in vivo functions of , we implemented a CRISPR-Cas9 gene editing strategy to delete a small region encompassing the distal poly(A) site. This eliminated expression while maintaining expression of Mice lacking ( ) exhibited disorganized DRG migration in embryos, and reduced experience-induced neuronal activation in the adult hippocampus. These data indicate that plays functional roles in the central and peripheral nervous systems.
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http://dx.doi.org/10.1261/rna.076430.120DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7491327PMC
October 2020
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