Publications by authors named "Ting Chen"

1,560 Publications

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[Melatonin Induced Apoptosis of RPMI 8226 Cells through Endoplasmic Reticulum Stress].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2022 Aug;30(4):1156-1161

Department of Hematology, Guizhou Medical University Affiliated Hospital, Guiyang 550004, Guizhou Province,

Objective: To investigate the effect of melatonin (MLT) on the proliferation and apoptosis of human multiple myeloma cell line RPMI 8226 and its possible mechanism.

Methods: RPMI 8226 cells were cultured in vitro, and different concentrations of MLT were treated on RPMI 8226 cells. The effects of MLT on RPMI 8226 cell proliferation were detected by CCK-8 assay and methylcellulose cloning assay, and the effects of MLT on cell apoptosis were detected by AnnexinV-FITC /PI, flow cytometry. Western blot was used to determine the expression of apoptosis and endoplasmic reticulum stress-related proteins in each group, and CCK-8 assay was used to determine the effect of MLT combined with bortezemib on the viability of RPMI 8226 cells.

Results: MLT inhibited the proliferation of RPMI 8226 cells in a dose- and time-dependent manner (r=-0.9777,r=-0.9951). With the increase of MLT concentration, the number of clones decreased, the apoptosis of RPMI 8226 cells increased (P<0.05), the expression of anti-apoptotic protein XIAP decreased, the expression of apoptotic proteins Bax and Caspase3 increased, and the expression of endoplasmic reticulum stress-related proteins increased. Compared with the control group, the survival of RPMI 8226 cells in the MLT and BTZ combined group significantly decreased (P<0.01).

Conclusion: MLT can inhibit the proliferation of RPMI 8226 cells, promote the apoptosis of RPMI 8226 cells, and enhance the anti-tumor effect of BTZ on RPMI 8226 cells. The mechanism may be related to endoplasmic reticulum stress.
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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2022.04.028DOI Listing
August 2022

[Clinical Characteristics of Multiple Myeloma Patients with Early Relapse].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2022 Aug;30(4):1144-1149

Department of Hematology, Jiangsu Province Hospital, Nanjing 210000, Jiangsu Province, China,E-mail:

Objective: To analyze the clinical characteristics of multiple myeloma(MM) patients with early relapse.

Methods: A total of 50 MM patients with early relapse (≤12 months) and 50 matched controls with late relapse (>12 months) were selected. The time from diagnosis to relapse and related clinical data of the 100 patients were retrospectively analyzed, and the factors associated with early relapse were identified. Kaplan-Meier curve was used to analyze the overall survival (OS) time of the whole cohort. Area under the curve (AUC) was used to evaluate the effect of circulating plasma cells on early recurrence of the patients.

Results: The results showed that high-risk cytogenetics (FISH) (P=0.005), and ISS stage III (P=0.008) were associated with early recurrence of the patients. For patients with early relapse, high-risk FISH showed poor survival. Compared with the patients with late relapse, most of the chromosome karyotype of patients with early relapse showed quantitative and structural abnormalities. The expression of circulating plasma cells was significantly increased in patients with early recurrence group (P=0.0318). The response to initial treatment was poor in the early recurrence group (P=0.001), and the prognosis was significantly worse than those in the late recurrence group (median OS: 38 vs 81 months, P=0.002).

Conclusion: Early relapse is a marker poor prognostic in MM patients, and such patients should be focused on the improving their prognosis.
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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2022.04.026DOI Listing
August 2022

Ultra-Low Concentration Electrolyte Enabling LiF-Rich SEI and Dense Plating/Stripping Processes for Lithium Metal Batteries.

Adv Sci (Weinh) 2022 Aug 17:e2203216. Epub 2022 Aug 17.

Department of Chemical Engineering, Sichuan University, Chengdu, 610065, P. R. China.

The interface structure of the electrode is closely related to the electrochemical performance of lithium-metal batteries (LMBs). In particular, a high-quality solid electrode interface (SEI) and uniform, dense lithium plating/stripping processes play a key role in achieving stable LMBs. Herein, a LiF-rich SEI and a uniform and dense plating/stripping process of the electrolyte by reducing the electrolyte concentration without changing the solvation structure, thereby avoiding the high cost and poor wetting properties of high-concentration electrolytes are achieved. The ultra-low concentration electrolyte with an unchanged Li solvation structure can restrain the inhomogeneous diffusion flux of Li , thereby achieving more uniform lithium deposition and stripping processes while maintaining a LiF-rich SEI. The LiIICu battery with this electrolyte exhibits enhanced cycling stability for 1000 cycles with a coulombic efficiency of 99% at 1 mA cm and 1 mAh cm . For the LiIILiFePO pouch cell, the capacity retention values at 0.5 and 1 C are 98.6% and 91.4%, respectively. This study offers a new perspective for the commercial application of low-cost electrolytes with ultra-low concentrations and high concentration effects.
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http://dx.doi.org/10.1002/advs.202203216DOI Listing
August 2022

Characterization of anaerobic oxidation of methane and microbial community in landfills with aeration.

Environ Res 2022 Aug 13:114102. Epub 2022 Aug 13.

Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou, 310012, China. Electronic address:

Landfills are the third largest source of anthropogenic CH emissions. Anaerobic oxidation of methane (AOM) activity and communities of methane-oxidizing bacteria were investigated in three informal landfills in this study, namely, BJ, CH and SZ landfills, among which BJ and CH represent traditional anaerobic landfills, while the SZ landfill was subjected to aeration to accelerate waste stabilization. The AOM rates of the investigated landfilled wastes ranged from 3.66 to 23.91 nmol g h. Among the three landfills, the AOM rate was highest in the SZ-1-Top sample, which was closest to the aeration pipe. Among the possible electron acceptors for AOM, including NO, NO, SO and Fe, the NO-N content was the only variable that was positively correlated with the AOM rate. Compared with α-Proteobacteria methanotrophs, γ-Proteobacteria methanotrophs were more abundant in the landfilled waste, especially Methylobacter, which was detected in nearly all samples. Members of the family Methylomirabilaceae, including Candidatus Methylomirabilis, were also detected in the SZ-1 and SZ-2-Bot samples. The relative abundance of the main methanotrophs in the families Methylomonadaceae, Methylococcaceae, Rokubacteriales and Methylomirabilaceae, the genus Methylocystis and the phylum NC10 were all positive correlations with the contents of NO-N in the landfilled waste samples. Additionally, significantly positive correlations were observed between the AOM rates and the relative abundance of the main methanotrophs except for the family Methylococcaceae. This indicated that aeration could enhance the conversion of nitrogen compounds in the landfilled waste, in which the high contents of NO-N could stimulate the growth of methanotrophs and increase AOM rate. These findings are helpful for understanding the mechanisms of CH oxidation in landfills and for taking effective measures to mitigate CH emissions from landfills.
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http://dx.doi.org/10.1016/j.envres.2022.114102DOI Listing
August 2022

CDC20 is a novel biomarker for improved clinical predictions in epithelial ovarian cancer.

Am J Cancer Res 2022 15;12(7):3303-3317. Epub 2022 Jul 15.

Department of Obstetrics and Gynaecology, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Gusu School, Nanjing Medical University Suzhou 215002, Jiangsu, China.

Epithelial ovarian cancer (EOC), a common tumor of the female reproductive system, ranks first in fatalities among gynecological malignancies. Most patients find tumors at late stage and have extremely poor prognoses, which necessitates improvements in early detection. This study applied bioinformatic methods to identify potential biomarkers of EOC. First, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed on differentially expressed genes (DEGs) and hub genes, and a protein-protein interaction (PPI) network was constructed. The network of hub genes was analyzed using GeneMANIA, and an analysis of biological processes was constructed with BINGO. Lastly, hub genes were analyzed for EOC-related oncology using the Oncomine and TCGA databases, and the cBioPortal online platform. Overall, cell division cycle 20 (CDC20) was identified as a key gene in EOC. Short hairpin RNA (shRNA) was used to silence CDC20 to explore its effects on EOC cell proliferation, apoptosis and SRY-related HMG-box 2 (SOX2) expression. DEGs were enriched in pathways related to cell cycle signaling, cancer, progesterone-mediated oocyte maturation, Wnt signaling and P53 signaling. Analysis revealed high expression of CDC20 in EOC tissues and a correlation with histology and tumor grade. CDC20 levels are highest in serous adenocarcinoma, when compared to ovarian clear cell carcinoma, ovarian endometrioid carcinoma and mucinous adenocarcinoma. High CDC20 expression within the tumor is associated with poor EOC prognosis. After silencing , EOC cell proliferation and migration decreased, apoptosis increased, and expression decreased. In conclusion, CDC20 is likely a key biomarker of EOC and may act as an upstream regulator of SOX2 to mediate the SOX2 signaling in the progression of EOC. Future application of CDC20 analysis to early detection may improve prognosis, and it has the potential to be a therapeutic target.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9360218PMC
July 2022

Investigation and experimental validation of curcumin-related mechanisms against hepatocellular carcinoma based on network pharmacology.

J Zhejiang Univ Sci B 2022 Aug;23(8):682-698

School of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang 712046, China.

Objectives: To determine the potential molecular mechanisms underlying the therapeutic effect of curcumin on hepatocellular carcinoma (HCC) by network pharmacology and experimental in vitro validation.

Methods: The predictive targets of curcumin or HCC were collected from several databases. the identified overlapping targets were crossed with Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses using the Database for Annotation, Visualization, and Integrated Discovery (DAVID) platform. Two of the candidate pathways were selected to conduct an experimental verification. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide tetrazolium (MTT) assay was used to determine the effect of curcumin on the viability of HepG2 and LO2 cells. The apoptosis and autophagy of HepG2 cells were respectively detected by flow cytometry and transmission electron microscopy. Besides, western blot and real-time polymerase chain reaction (PCR) were employed to verify the p53 apoptotic pathway and adenosine 5'-monophosphate (AMP)‍-activated protein kinase (AMPK) autophagy pathway. HepG2 cells were pretreated with pifithrin-‍α (PFT-‍α) and GSK690693 for further investigation.

Results: The 167 pathways analyzed by KEGG included apoptosis, autophagy, p53, and AMPK pathways. The GO enrichment analysis demonstrated that curcumin was involved in cellular response to drug, regulation of apoptotic pathway, and so on. The in vitro experiments also confirmed that curcumin can inhibit the growth of HepG2 cells by promoting the apoptosis of p53 pathway and autophagy through the AMPK pathway. Furthermore, the protein and messenger RNA (mRNA) of the two pathways were downregulated in the inhibitor-pretreated group compared with the experimental group. The damage-regulated autophagy modulator (DRAM) in the PFT-‍α-pretreated group was downregulated, and p62 in the GSK690693-pretreated group was upregulated.

Conclusions: Curcumin can treat HCC through the p53 apoptotic pathway and the AMPK/Unc-51-like kinase 1 (ULK1) autophagy pathway, in which the mutual transformation of autophagy and apoptosis may occur through DRAM and p62.
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http://dx.doi.org/10.1631/jzus.B2200038DOI Listing
August 2022

Proof of concept for developing novel feeds for cattle from wasted food and crop biomass to enhance agri-food system efficiency.

Sci Rep 2022 Aug 10;12(1):13630. Epub 2022 Aug 10.

Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, 382 W. Street Rd., Kennett Square, PA, 19348, USA.

Modern agri-food systems generate large amounts of crop-based biomass that are unfit for direct human consumption but potentially suitable for livestock feeding in production of meats, milk, and eggs. This study aims to develop novel feeds for cattle from some of those biomass materials through the natural microbial-driven processes of ensiling. Fruit and vegetables resembling supermarket discards were ensiled alone or co-ensiled with corn crop residues, mushroom wastes, etc. via laboratory experiments. Longitudinal sample analyses showed that (co-)ensiling was successful, with pH and fermentation acids changing rapidly into desirable ranges (pH < 4.5, the acids 5-13% DM with lactic acid dominating). The (co-)ensiled products had key nutritional parameters comparable to those of good quality forages commonly used on dairy farms. Additionally, in vitro incubation experiments indicated that the ensiled products could substitute certain conventional feeds while maintaining diet digestibility. Findings from this pilot study provide a proof of principle that quality novel feeds for cattle can be generated by co-ensiling food discards and low-value crop residues. Future research and animal feeding trials to demonstrate the utility of this approach can help societies more effectively utilize untapped biomass resources, strengthening the regenerative capacity of agri-food systems towards a more sustainable food future.
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http://dx.doi.org/10.1038/s41598-022-17812-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9365796PMC
August 2022

Cost-Utility Analysis of Camrelizumab Plus Chemotherapy Versus Chemotherapy Alone as a First-Line Treatment for Advanced Nonsquamous Non-Small Cell Lung Cancer in China.

Front Oncol 2022 22;12:746526. Epub 2022 Jul 22.

Department of Pharmacy, Fujian Medical University Cancer Hospital, Fujian Cancer Hospital, Fuzhou, China.

Purpose: To evaluate the cost utility of camrelizumab plus standard chemotherapy versus standard chemotherapy alone as a first-line treatment for advanced nonsquamous non-small cell lung cancer (NSCLC) from the perspective of the Chinese health care system and to provide a reference for health decision-making.

Methods: A Markov model consisting of three health states was designed to evaluate the cost utility of these two treatment regimens for NSCLC patients with the incremental cost-effectiveness ratio (ICER) as the primary output indicator. Clinical data were derived from a published phase III clinical trial (CameL; ClinicalTrials.gov; NCT03134872). One-way sensitivity analysis and probabilistic sensitivity analysis were performed to assess the model uncertainty.

Results: Base case analysis showed that the ICER of camrelizumab plus chemotherapy compared with chemotherapy alone was $43,275.43 per QALY. It was higher than the willingness-to-pay (WTP) threshold of $31,510.57 per QALY in China, which has a standard of three times the GDP per capita recommended by the WHO. One-way sensitivity analysis showed that the utility value of PFS had the greatest influence on the results, and the other sensitive parameters were the cost of subsequent second-line therapy in the two group, the pemetrexed price, the cost of adverse event management and the utility value of PD. The probability sensitivity analysis showed that the probabilities of the cost-effectiveness of camrelizumab plus standard chemotherapy were 27.1%, 66.7% and 88.0% when the WTP values were $40,000, $50,000 and $60,000 per QALY, respectively.

Conclusions: Taking three times the GDP per capita in China as the WTP threshold, the camrelizumab plus standard chemotherapy regimen does not have a cost-effectiveness advantage compared with the standard chemotherapy regimen alone as a first-line treatment for advanced NSCLC.
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http://dx.doi.org/10.3389/fonc.2022.746526DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353739PMC
July 2022

Directional Manipulation of Electron Transfer by Energy Level Engineering for Efficient Cathodic Oxygen Reduction.

Nano Lett 2022 Aug 5. Epub 2022 Aug 5.

School of Chemistry and Materials Science, Collaborative Innovation Center of Chemistry for Energy Materials, University of Science and Technology of China, Hefei, Anhui 230026, P.R. China.

Electron transfer plays an important role in determining the energy conversion efficiency of energy devices. Nitrogen-coordinated single metal sites (M-N) materials as electrocatalysts have exhibited great potential in devices. However, there are still great difficulties in how to directionally manipulate electron transfer in M-N catalysts for higher efficiency. Herein, we demonstrated the mechanism of electron transfer being affected by energy level structure based on classical iron phthalocyanine (FePc) molecule/carbon models and proposed an energy level engineering strategy to manipulate electron transfer, preparing high-performance ORR catalysts. Engineering molecular energy level via modulating FePc molecular structure with nitro induces a strong interfacial electronic coupling and efficient charge transfer from carbon to FePc-β-NO molecule. Consequently, the assembled zinc-air battery exhibits ultrahigh performance which is superior to most of M-N catalysts. Energy level engineering provides a universal approach for directionally manipulating electron transfer, bringing a new concept to design efficient and stable M-N electrocatalyst.
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http://dx.doi.org/10.1021/acs.nanolett.2c01933DOI Listing
August 2022

Aromatic Ring Fusion to Benzoporphyrin γ- Cyclodehydrogenation on a Ag(111) Surface.

ACS Nano 2022 Aug 1. Epub 2022 Aug 1.

Key Laboratory of Molecular Nanostructure and Nanotechnology and Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences (CAS), Beijing 100190, People's Republic of China.

Aromatic ring fusion to porphyrins and their derivatives represents an attractive route to tune the molecular conjugation and thus expand their functionalities. Here, we report the expansion of the aromatic π-system of palladium tetraphenyltetrabenzoporphyrins (Pd-TPTBP) surface-assisted γ- cyclodehydrogenation on Ag(111). The chemical transformation of Pd-TPTBP into different products at an elevated temperature of 600 K was revealed at the single-molecule level using bond-resolved scanning tunneling microscopy with a CO-functionalized tip. We captured a series of γ cyclodehydrogenation products, wherein the maximum extent to which the reaction can progress is associated with 7-fold C-C formation to afford nearly planar γ- fused porphyrins with 66 conjugated π-electrons. In addition, a small number of molecules undergo C-C bond dissociation of -phenyl at elevated temperature, producing fully planar γ- fused products lacking one or two phenyl moieties. Scanning tunneling spectroscopy measurements and DFT calculations suggest the electronic gap of the γ fused porphyrin decreases compared to that of the precursor. The HOMO and LUMO of the planar γ- fused products are localized on the partially fused benzo moieties and the -position, respectively.
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http://dx.doi.org/10.1021/acsnano.2c05819DOI Listing
August 2022

Evolutionarily Ancient Caspase-9 Sensitizes Immune Effector Coelomocytes to Cadmium-Induced Cell Death in the Sea Cucumber, .

Front Immunol 2022 14;13:927880. Epub 2022 Jul 14.

CAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences (CAS), Guangzhou, China.

Heavy-metal pollution has increasingly jeopardized the habitats of marine organisms including the sea cucumber, a seafloor scavenger vital to seawater bio-decontamination, ocean de-acidification and coral-reef protection. Normal physiology including immune functions of sea cucumbers is toxicologically modulated by marine metal pollutants such as cadmium (Cd). The processes underpinning Cd's toxic effects on immune systems in the sea cucumber, , are still poorly understood. To this end, we cloned and characterized a full-length caspase-9 () cDNA in the sea cucumber, . mRNA levels evolved dynamically during embryonic development. Coelomocytes, a type of phagocytic immune effectors central to immunity, were found to express mRNA most abundantly. Hl-CASP9 protein structurally resembles caspases-2 and -9 in both invertebrate and vertebrate species, comprising a CARD domain and a CASc domain. Remarkably, was transcriptionally sensitive to abiotic oxidative stress inducers including hydrogen peroxide (HO), nitric oxide (NO) and cadmium (Cd), but insensitive to immunostimulants including lipopolysaccharide (LPS), and poly(I:C). Overexpression of augmented mitochondria-dependent apoptosis in HEK293T cells, while knock-down of blunted Cd-induced coelomocyte apoptosis . Overall, we illustrate that an evolutionarily ancient caspase-9-dependent pathway exists to sensitize coelomocytes to premature cell death precipitated by heavy metal pollutants, with important implications for negative modulation of organismal immune response in marine invertebrates.
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http://dx.doi.org/10.3389/fimmu.2022.927880DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330033PMC
August 2022

Purple red rice anthocyanins alleviate intestinal damage in cyclophosphamide-induced mice associated with modulation of intestinal barrier function and gut microbiota.

Food Chem 2022 Jul 25;397:133768. Epub 2022 Jul 25.

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. Electronic address:

The regulatory effects of purple red rice bran anthocyanins (PRBA) on intestinal barrier function and gut microbiota in mice were investigated. Results showed that PRBA had an ameliorative effect on intestinal barrier damage, including restoration of villus length, improvement in the number of cupped cells and promotion of sIgA secretion. PRBA stimulated the production of cytokines, reduced the levels of endotoxin (ET) and lipopolysaccharide binding protein (LBP) in serum, as well as upregulated the expression of tight junction proteins (TJs) and NF-κB pathway proteins. Furthermore, PRBA not only promoted the production of short-chain fatty acids (SCFAs), but also regulated the intestinal microbiota by increasing beneficial bacteria (Lachnospiraceae, Bacteroidaceae, Ruminococcaceae) and reducing pathogenic bacteria (Shigella) to maintained intestinal homeostasis. Above results indicated that PRBA could ameliorate cyclophosphamide-induced impairment of intestinal barrier function and dysregulation of the gut microbiota, which provides a new idea for broadening the exploitation of PRBA.
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http://dx.doi.org/10.1016/j.foodchem.2022.133768DOI Listing
July 2022

Oxysterol derivatives Oxy186 and Oxy210 inhibit WNT signaling in non-small cell lung cancer.

Cell Biosci 2022 Jul 30;12(1):119. Epub 2022 Jul 30.

Laboratory of Cellular and Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, NIH, Building 37, RM 2056B, Bethesda, MD, 20892, USA.

Background: Developmental signaling pathways such as those of Hedgehog (HH) and WNT play critical roles in cancer stem cell self-renewal, migration, and differentiation. They are often constitutively activated in many human malignancies, including non-small cell lung cancer (NSCLC). Previously, we reported that two oxysterol derivatives, Oxy186 and Oxy210, are potent inhibitors of HH/GLI signaling and NSCLC cancer cell growth. In addition, we also showed that Oxy210 is a potent inhibitor of TGF-β/SMAD signaling. In this follow-up study, we further explore the mechanism of action by which these oxysterols control NSCLC cell proliferation and tumor growth.

Results: Using a GLI-responsive luciferase reporter assay, we show here that HH ligand could not mount a signaling response in the NSCLC cell line A549, even though Oxy186 and Oxy210 still inhibited non-canonical GLI activity and suppressed the proliferation of A549 cells. Further, we uncover an unexpected activity of these two oxysterols in inhibiting the WNT/β-catenin signaling at the level of LRP5/6 membrane receptors. We also show that in a subcutaneous xenograft tumor model generated from A549 cells, Oxy186, but not Oxy210, exhibits strong inhibition of tumor growth. Subsequent RNA-seq analysis of the xenograft tumor tissue reveal that the WNT/β-catenin pathway is the target of Oxy186 in vivo.

Conclusion: The oxysterols Oxy186 and Oxy210 both possess inhibitory activity towards WNT/β-catenin signaling, and Oxy186 is also a potent inhibitor of NSCLC tumor growth.
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http://dx.doi.org/10.1186/s13578-022-00857-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338492PMC
July 2022

Effect of magnetite particle size on propionate degradation in the propionate-based anaerobic system.

Sci Total Environ 2022 Jul 25;847:157592. Epub 2022 Jul 25.

School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, PR China; Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, Hangzhou 310012, PR China; Instrumental Analysis Center of Zhejiang Gongshang University, Hangzhou 310018, PR China. Electronic address:

The size effect of magnetite (FeO) on the degradation of propionate (PA) in the PA-based anaerobic system was investigated. The sequential bench-scale experiments were conducted. Results showed that the effects of different sized magnetite particles on PA degradation varied, and reaction cycles also played a role in substrate removal/degradation. With the increase of reaction cycle, nano-magnetite promoted PA degradation and CH production, which caused faster PA degradation rate (0.997 g/L·d) than the control group (CK) without magnetite (0.834 g/L·d), whereas the groups with micron- and millimeter-sized magnetite had slower PA degradation rates (0.746 and 0.636 g/L·d) than CK group. The particle size or surface characteristics of the magnetite may become the main factor determining the PA degradation rate. Furthermore, the analysis of PA conversion and volatile fatty acids (VFAs) distribution showed the C6-dismutation pathway, which converses PA to butyrate, enhanced by the introduction of magnetite. Microbial community analysis showed that PA was degraded mainly by methyl-malonyl-CoA (MMC) pathway. The relative abundance of Syntrophobacter that catalyze MMC pathway in the group with nano-magnetite were much higher after three reaction cycles at 39 %, as compared to micro-magnetite at 28 %, and millimeter-sized magnetite at 27 %, which contributed to faster degradation of PA. Functional enzyme-encoding genes for the four methanogenesis pathways were identified with reference to KEGG database entries. The methanogenesis pathway using acetate was the most abundant pathway in all groups. The observations have important implications for enhancing the PA removal in PA-inhibited anaerobic digester.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157592DOI Listing
July 2022

Preventive strategies for feeding intolerance among patients with severe traumatic brain injury: A cross-sectional survey.

Int J Nurs Sci 2022 Jul 16;9(3):278-285. Epub 2022 Jun 16.

Department of Basic Nursing, School of Nursing, Army Medical University, Chongqing, China.

Objectives: This study aimed to investigate the application status of preventive measures for feeding intolerance in patients with severe traumatic brain injury (STBI) in China and analysis the differences and their causes.

Methods: A cross-sectional survey was conducted. From December 2019 to January 2020, ICU nurses and physicians of 89 hospitals in China were surveyed by using a questionnaire on preventive strategies for feeding intolerance in patients with STBI. The questionnaire included two parts: the general information of participants (10 items) and application of preventive measures for feeding intolerance in STBI patients (18 items).

Results: Totally 996 nurses and physicians completed the questionnaire. Among various methods, gastrointestinal symptoms(85.0%) and injury severity (71.4%) were mostly used to assess gastrointestinal functions and risk of feeding intolerance among STBI patients, respectively. Initiating enteral nutrition (EN) within 24-48 h (61.5%), nasogastric tubes (91.2%), 30°-45° of head-of-bed elevation (89.5%), continuous feeding by pump (72.9%), EN solution temperature of 38-40 °C (65.5%), <500 ml initial volume of EN solution (50.0%), monitoring gastric residual volume with a syringe (93.7%), and assessing gastric residual volume every 4 h (51.5%) were mostly applied for EN delivery among STBI patients. Prokinetic agents (73.3%), enema (73.6%), probiotics (79.0%), antacid agents (84.1%), and non-nutritional preparations as initial EN formula (65.6%) were commonly used for preventing feeding intolerance among STBI patients.

Conclusions: The survey showed that nurses and clinicians in China have a positive attitude towards preventive strategies for feeding intolerance. However, some effective new technologies and methods have not been timely applied in clinical practice. We suggest that managers, researchers, clinicians, nurses, and other health professionals should collaborate to explore effective and standard preventive strategies for feeding intolerance among patients with STBI.
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http://dx.doi.org/10.1016/j.ijnss.2022.06.014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304998PMC
July 2022

Two Novel Co-Crystals of Naproxen: Comparison of Stability, Solubility and Intermolecular Interaction.

Pharmaceuticals (Basel) 2022 Jun 29;15(7). Epub 2022 Jun 29.

Beijing City Key Laboratory of Polymorphic Drugs, Center of Pharmaceutical Polymorphs, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.

Two novel co-crystals of naproxen (NPX) were designed and prepared at a stoichiometric ratio of 1:1, namely, naproxen-caprolactam (NPX-CPL) and naproxen-oxymatrine (NPX-OMT). The characteristics of the co-crystals were evaluated in terms of stability and solubility studies. In terms of solubility, in four kinds of solvent systems with different pH, the solubility of NPX-OMT was significantly improved compared with that of NPX, whereas the NPX-CPL showed advantages in acidic solvent systems, indicating that the co-crystals can be applied to concoct preparations depending on therapeutic purposes. Furthermore, the experimental results of the thermal analysis showed that the co-crystal NPX-OMT had better thermal stability than the co-crystal NPX-CPL. Finally, as a complement to the single crystal X-ray diffraction (SC XRD) method, the theoretical calculation based on density functional theory (DFT) was also used to reveal the intermolecular interaction of the co-crystals at the molecular level and visually display the difference between them.
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http://dx.doi.org/10.3390/ph15070807DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317554PMC
June 2022

Curcumol Synergizes with Cisplatin in Osteosarcoma by Inhibiting M2-like Polarization of Tumor-Associated Macrophages.

Molecules 2022 Jul 6;27(14). Epub 2022 Jul 6.

Department of Pharmacy, Hangzhou Medical College, Hangzhou 310053, China.

Osteosarcoma is the most prevalent bone cancer, and chemotherapy is still an indispensable treatment in its clinical practice. Cisplatin (CDDP) has become the most commonly used agent for osteosarcoma, although the outcomes of CDDP chemotherapy remain unsatisfactory because of frequent resistance. Here, we report on a promising combination therapy where curcumol, a bioactive sesquiterpenoid, enhanced CDDP-induced apoptosis to eradicate osteosarcoma cells, and revealed that M2-like macrophages might be the underlying associated mechanisms. First, we observed that curcumol enhanced the CDDP-mediated inhibition of cell proliferation and augmented the apoptosis in osteosarcoma cell lines. Curcumol contributed to preventing the migration of osteosarcoma cells when combined with CDDP. Moreover, this drug combination showed more potent tumor-growth suppression in the orthotopic transplantation of osteosarcoma K7M2 WT cells. We then estimated chemotherapy-associated drug-resistant genes, including ABCB1, ABCC1 and ABCG2, and found that curcumol significantly reversed the mRNA levels of CDDP-induced ABCB1, ABCC1 and ABCG2 genes in the tumor tissue. Moreover, M2-like macrophages were enriched in osteosarcoma tissues, and were largely decreased after curcumol and CDDP treatment. Taken together, these findings suggest that curcumol inhibits the polarization of M2-like macrophages and could be a promising combination strategy to synergize with CDDP in the osteosarcoma.
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http://dx.doi.org/10.3390/molecules27144345DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318016PMC
July 2022

Low-Loss Dual-Band Transparency Metamaterial with Toroidal Dipole.

Materials (Basel) 2022 Jul 19;15(14). Epub 2022 Jul 19.

School of Big Data and Computer Science, Guizhou Normal University, Guiyang 550003, China.

In this paper, a low-loss toroidal dipole metamaterial composed of four metal split ring resonators is proposed and verified at microwave range. Dual-band Fano resonances could be excited by normal incident electromagnetic waves at 6 GHz and 7.23 GHz. Analysis of the current distribution at the resonance frequency and the scattered power of multipoles shows that both Fano resonances derive from the predominant novel toroidal dipole. The simulation results exhibit that the sensitivity to refractive index of the analyte is 1.56 GHz/RIU and 1.8 GHz/RIU. Meanwhile, the group delay at two Fano peaks can reach to 11.38 ns and 12.85 ns, which means the presented toroidal metamaterial has significant slow light effects. The proposed dual-band toroidal dipole metamaterial may offer a new path for designing ultra-sensitive sensors, filters, modulators, slow light devices, and so on.
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http://dx.doi.org/10.3390/ma15145013DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317833PMC
July 2022

Effects of Social Participation by Middle-Aged and Elderly Residents on the Utilization of Medical Services: Evidence From China.

Front Public Health 2022 7;10:824514. Epub 2022 Jul 7.

School of Public Health, Hubei Provincial Key Laboratory of Occupational Hazard Identification and Control, Wuhan University of Science and Technology, Wuhan, China.

Objectives: Aim to evaluate the effect of social participation on utilization of medical services among middle-aged and elderly residents in China.

Methods: We used data from the 2018 wave of the China Health and Retirement Longitudinal Study. Social participation is classified into three types. Furthermore, to control for confounding factors, our study computed propensity score matching (PSM) to evaluate the effect of social participation on the utilization of medical services.

Result: The result of PSM indicates that social participation significantly positively affects the utilization of outpatient services, the average treatment effect on the treated (ATT = 0.038) and the utilization of inpatient services (ATT = 0.015) by middle-aged and elderly residents. Furthermore, the utilization of outpatient health care services was significantly positively associated with leisure activities (ATT = 0.035), social activities to help others (ATT = 0.031), and learning activities to gain new knowledge (ATT = 0.034) among middle-aged and elderly residents. The utilization of inpatient health care was significantly positively associated with leisure activities (ATT = 0.015) but had no significant association with social deeds that help others and increased new knowledge among middle-aged and elderly residents.

Conclusion: Thus, social participation significantly positively affects healthcare utilization by middle-aged and elderly residents. Hence, the government and society should provide more conveniences and promote social participation among middle-aged and elderly residents.
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http://dx.doi.org/10.3389/fpubh.2022.824514DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301239PMC
July 2022

Highly ordered asymmetric cellulose-based honeycomb membrane for moisture-electricity generation and humidity sensing.

Carbohydr Polym 2022 Oct 3;294:119809. Epub 2022 Jul 3.

College of Bioresources Chemical and Materials Engineering, Shaanxi Province Key Laboratory of papermaking Technology and Specialty paper Development, Shaanxi University of Science & Technology, Xi'an 710021, China. Electronic address:

Moisture-trigged electricity generator (MEG) that can convert ubiquitous moisture into electricity are highly desirable for developing renewable energy supply and ameliorating the crisis in energy. Constructing an asymmetric ordered, namely gradient ordered porous membrane has great potential in MEG. Herein, a series of cellulose acetate (CA)-based membranes with ordered asymmetric honeycomb membranes were fabricated by Breath Figure method, along with silver nanowires (AgNWs) coating. The asymmetric gradient honeycomb pores were achieved by graft modification of lauroyl chloride and adjustment of relative humidity, which not only endowed the MEG with sensitive sensing signals transport under tension and humidity fluctuations but also enhanced voltages generation speed under flowing moisture. The current study provides a facile and scalable strategy for constructing asymmetric gradient ordered porous materials and creates more possibilities for MEG self-powered flexible wearable electronic devices.
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http://dx.doi.org/10.1016/j.carbpol.2022.119809DOI Listing
October 2022

Cooling-activated dorsomedial hypothalamic BDNF neurons control cold defense in mice.

J Neurochem 2022 Jul 21. Epub 2022 Jul 21.

School of Life Science and Technology, Shanghaitech University, Shanghai, China.

BDNF and its expressing neurons in the brain critically control feeding and energy expenditure (EE) in both rodents and humans. However, whether BDNF neurons would function in thermoregulation during temperature challenges is unclear. Here, we show that BDNF neurons in the dorsomedial hypothalamus (DMH ) of mice are activated by afferent cooling signals. These cooling-activated BDNF neurons are mainly GABAergic. Activation of DMH neurons or the GABAergic subpopulations is sufficient to increase body temperature, EE, and physical activity. Conversely, blocking DMH neurons substantially impairs cold defense and reduces energy expenditure, physical activity, and UCP1 expression in BAT, which eventually results in bodyweight gain and glucose/insulin intolerance. Therefore, we identify a subset of DMH neurons as a novel type of cooling-activated neurons to promote cold defense. Thus, we reveal a critical role of BDNF circuitry in thermoregulation, which deepens our understanding of BDNF in controlling energy homeostasis and obesity.
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http://dx.doi.org/10.1111/jnc.15666DOI Listing
July 2022

The effects of dexmedetomidine on the cognitive function of mild cognitive impairment (MCI) rats.

Ann Transl Med 2022 Jun;10(12):667

Department of Anesthesiology, the First Affiliated Hospital of Fujian Medical University, Fuzhou, China.

Background: The study sought to investigate the effects of dexmedetomidine (DEX) on cognitive function after anesthesia and to examine its actual mechanism.

Methods: A total of 48 rats were injected with d-galactose (D-gal) 1,000 mg·kg·d and normal saline at the neck and back for 1 week to establish rats with mild cognitive impairment (MCI) and conduct behavioral tests. Sevoflurane was inhaled and DEX was pumped into each group respectively. Morris water maze (MWM) test was conducted 24 hours later. The inflammatory factors interleukin (IL)-1, interleukin (IL)-6, and a tumor necrosis factor (TNF)-α in brain homogenate were quantitatively measured by enzyme-linked immunosorbent assay (ELISA) on the next day. The apoptosis of hippocampal cells was observed by hematoxylin-eosin staining (HE staining).

Results: In relation to the model establishment, we found that there was no significant difference in body weight and swimming speed before and after modeling. There was no statistically significant difference in the escape latency between Groups A, B, C, and D before modeling. After modeling, there was no statistical difference in the escape latency between Groups A, B, and C, but the difference was statistically significant when compared to Group D (P<0.05). In relation to the DEX intervention, we found that compared to Group C, MWM test performance in Groups A and B was considerably worse longer escape latencies and fewer platform crossings within 90 seconds), and were more significant in Group A. Compared with Group D, the levels of inflammatory cytokines of the brain homogenates were elevated, and this elevation was highest in Group A, followed by Group B; the pathological changes were consistent with changes in behavioral tests. In Group A, there were obvious disorders of glial cell arrangement, apoptosis and deletion. There was no significant change in Group D. And the changes of vertebral cells in Group B and Group C were slight, with orderly arrangement and intact cell structure.

Conclusions: DEX inhibits the apoptosis of hippocampal cells and reduces the cognitive dysfunction of rats with MCI induced by D-gal via the inhibition of the release of inflammatory cytokines.
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http://dx.doi.org/10.21037/atm-22-2043DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279787PMC
June 2022

Influence of Three-Dimensional Visual Reconstruction Technology Combined with Virtual Surgical Planning of CTA Images on Precise Resection of Liver Cancer in Hepatobiliary Surgery.

Comput Math Methods Med 2022 7;2022:4376654. Epub 2022 Jul 7.

Department of Liver Surgery, Changzheng Hospital, Naval Medical University, Shanghai 200003, China.

Hepatobiliary malignancies, such as hepatocellular carcinoma (HCC) and biliary tract cancers, namely, gallbladder carcinoma and cholangiocarcinoma, are linked to a high rate of morbidity and mortality, depending on the phase of the disease. The intricate hepatobiliary anatomy and the need for accurate peroperative management, especially in patients with advanced liver disease, make these tumors difficult to treat. Surgical resection is a notable therapy for hepatobiliary cancers. Unnecessary or excessive liver excision influences patient rehabilitation, normal liver function, and postoperative complications. Hepatobiliary operations must therefore include accurate liver removal. The present advancements in imaging technology are aimed at improving the diagnostic efficacy of liver injury even more. Three-dimensional visual reconstruction is becoming more important in the diagnosis as well as treatment of a variety of disorders. In this paper, we proposed a novel three-dimensional visual reconstruction technology using enhanced nonuniform rational basis spline (ENURBS) combined with virtual surgical planning of Computed Tomography Angiography (CTA) images for precise liver cancer resection. The purpose of this project is to rebuild 2D CTA scan images of liver cancer into a 3D reconstructed model for efficient visualization and diagnosis of liver cancer and to prepare an effective preoperative surgical plan for precise liver excision based on a 3D recreated liver model. This method's performance is compared to that of 2D planning in terms of accuracy and time taken to complete the plan. It is concluded that our proposed technique outperforms the planning technique based on 2D images.
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http://dx.doi.org/10.1155/2022/4376654DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9283065PMC
July 2022

Impact of the COVID-19 pandemic on endoscopic procedures: a single-center study in China.

World J Emerg Med 2022 ;13(4):301-304

Department of Gastroenterology, the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China.

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http://dx.doi.org/10.5847/wjem.j.1920-8642.2022.065DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9233969PMC
January 2022

Comparison Analysis of Different DNA Extraction Methods on Suitability for Long-Read Metagenomic Nanopore Sequencing.

Front Cell Infect Microbiol 2022 28;12:919903. Epub 2022 Jun 28.

State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, China.

Metagenomic next-generation sequencing (mNGS) is a novel useful strategy that is increasingly used for pathogens detection in clinic. Some emerging mNGS technologies with long-read ability are useful to decrease sequencing time and increase diagnosed accuracy, which is of great significance in rapid pathogen diagnosis. Reliable DNA extraction is considered critical for the success of sequencing; hence, there is thus an urgent need of gentle DNA extraction method to get unbiased and more integrate DNA from all kinds of pathogens. In this study, we systematically compared three DNA extraction methods (enzymatic cell lysis based on MetaPolyzyme, mechanical cell lysis based on bead beating, and the control method without pre-cell lysis, respectively) by assessing DNA yield, integrity, and the microbial diversity based on long-read nanopore sequencing of urine samples with microbial infections. Compared with the control method, the enzymatic-based method increased the average length of microbial reads by a median of 2.1-fold [Inter Quartile Range (IQR), 1.7-2.5; maximum, 4.8) in 18 of the 20 samples and the mapped reads proportion of specific species by a median of 11.8-fold (Inter Quartile Range (IQR), 6.9-32.2; maximum, 79.27]. Moreover, it provided fully (20 of 20) consistent diagnosed results to the clinical culture and more representative microbial profiles ( < 0.05), which all strongly proves the excellent performance of enzymatic-based method in long-read mNGS-based pathogen identification and potential diseases diagnosis of microbiome related.
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http://dx.doi.org/10.3389/fcimb.2022.919903DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9273838PMC
July 2022

Reactions of Frustrated Lewis Pairs with Chloro-Diazirines: Cleavage of N=N Double Bonds.

Angew Chem Int Ed Engl 2022 Jul 13:e202209241. Epub 2022 Jul 13.

Institute of Drug Discovery Technology, Ningbo University, Ningbo, 315211, Zhejiang, China.

The reactions of FLPs with diazomethanes leads to the rapid loss of N . In contrast, in this work, we reported reactions of phosphine/borane FLPs with chlorodiazirines which led to the reduction of the N=N double bond, affording linked phosphinimide/amidoborate zwitterions of the general form R PNC(Ar)NR'BX(C F ) . A detailed DFT mechanistic study showed that these reactions proceed via FLP addition to the N=N bond, followed by subsequent group transfer reactions to nitrogen and capture of the halide anion.
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http://dx.doi.org/10.1002/anie.202209241DOI Listing
July 2022

Plant miR167e-5p promotes 3T3-L1 adipocyte adipogenesis by targeting β-catenin.

In Vitro Cell Dev Biol Anim 2022 Jun 12;58(6):471-479. Epub 2022 Jul 12.

College of Biological Chemical Sciences and Engineering, Jiaxing University, Jiaxing, 314000, China.

Adipogenesis is important in the development of fat deposition. Evidence showed that plant microRNAs (miRNAs) could be absorbed by the digestive tract and exert regulatory effects on animals' physiological processes. However, the regulation of plant miRNA on host lipogenesis remains unknown. This study explored the potential function of plant miRNA, miR167e-5p, in adipogenesis in vitro. The presentation of plant miR167e-5p improved lipid accumulation in 3T3-L1 cells. Bioinformatics prediction and luciferase reporter assay indicated that miR167e-5p targeted β-catenin. MiR167e-5p could not only negatively affect the expression of β-catenin but also showed a positive effect on several fat synthesis-related genes, peroxisome proliferator-activated receptor gamma (Pparγ), CCAAT/enhancer-binding protein α (Cebpα), fatty acid-binding protein 4 (Ap2), lipolysis genes, adipose triglyceride lipase (Atgl), and hormone-sensitive lipase (Hsl) messenger RNA levels. Meanwhile, lipid accumulation and the expression of the β-catenin and other five fat synthesis-related genes were recovered to their original pattern by adding the miR167e-5p inhibitor in 3T3-L1 cells. The immunoblot confirmed the same expression pattern in protein levels in β-catenin, PPAR-γ, FAS, and HSL. This research demonstrates that plant miR167e-5p can potentially affect adipogenesis through the regulation of β-catenin, suggesting that plant miRNAs could be a new class of bioactive ingredients in adipogenesis.
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http://dx.doi.org/10.1007/s11626-022-00702-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277600PMC
June 2022

Photodynamic therapy-a promising treatment of oral mucosal infections.

Photodiagnosis Photodyn Ther 2022 Jul 9;39:103010. Epub 2022 Jul 9.

State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Research Unit of Oral Carcinogenesis and Management, Chinese Academy of Medical Sciences, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, PR China.

The treatment of oral mucosal infections is increasingly challenging owing to antibiotic resistance. Therefore, alternative antimicrobial strategies are urgently required. Photodynamic therapy (PDT) has attracted attention for the treatment of oral mucosal infections because of its ability to effectively inactivate drug-resistant bacteria, completely heal clinical infectious lesions and usually offers only mild adverse reactions. This review briefly summarizes relevant scientific data and published papers and discusses the potential mechanism and application of PDT in the treatment of oral mucosal infections.
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http://dx.doi.org/10.1016/j.pdpdt.2022.103010DOI Listing
July 2022

An Argument-Based Validation of an Asynchronous Written Interaction Task.

Authors:
Ting Chen

Front Psychol 2022 22;13:889488. Epub 2022 Jun 22.

School of Foreign Languages, Shanghai Jiao Tong University, Shanghai, China.

Interactional competence has attracted increasing attention due to its significance for language users. Previous studies concerning interactional competence mainly focus on synchronous interaction tasks, while the utilization of asynchronous interaction tasks is relatively under-explored despite the importance of asynchronous interaction in real life. Taking the "Responding To Forum Posts" (RTFP) task used in the International Undergraduate English Entrance Examination (IUEEE) at Shanghai Jiao Tong University (SJTU) as an example, the study aims to validate the use of asynchronous interaction tasks in the assessment of learner's interactional competence. 49 students' performances on the RTFP task were collected through a prototype test of the IUEEE in 2021. The data were analyzed through content analysis, analysis of variance, and ordinal logistic regression. The results showed that the task elicited a wide range of interactional features and test-takers at different proficiency levels differed significantly in the variety of features and the amount of emotion-based interaction. The study also found significant correlations between some of the features and test takers' overall performance on interactional competence. The study has provided validity evidence for the RTFP task in the assessment of interactional competence and thrown light on the construct of asynchronous written interactional competence.
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http://dx.doi.org/10.3389/fpsyg.2022.889488DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9257166PMC
June 2022
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