Publications by authors named "Timothy O"

3 Publications

  • Page 1 of 1

The role of RANTES in a murine model of food allergy.

Immunol Invest 2004 Feb;33(1):27-38

The 2nd Department of Physiology, Aichi Medical University School of Medicine, Nagakute, Aichi, Japan.

Food allergy is an important and common health issue, and there is a need to identify and characterize the sensitizing mechanisms. One of the common causes of food allergy is ovalbumin (OVA), a dietary antigen from eggs. We hypothesized that OVA-induced food allergy in the gut involves the activation of the chemokine regulated on activation, normal T cell expressed and secreted (RANTES), which then recruits eosinophils to lesioned tissue. The purpose of this study was to clarify whether RANTES expression correlates with eosinophil infiltration in the gut of OVA-sensitized BALB/c mice in response to oral OVA challenge. BALB/c mice were immunized with OVA 1 microg and sensitized after 2 weeks by intragastric administration of OVA. Sensitization to the oral OVA challenge was analyzed by examining eosinophil infiltration into the gut tissue (immunohistochemistry), mucosal eosinophil cationic protein (ECP) concentration, and RANTES mRNA expression (reverse-transcriptase polymerase chain reaction and Southern blotting) at 3, 6, 12, and 24 h after the challenge. There was marked edema of the intestinal villi, and eosinophil infiltration to the lamina propria peaked at 6 h in OVA-sensitized mice. RANTES mRNA expression peaked at 3 h and 6 h and declined thereafter. The expression of RANTES mRNA in the allergic mice was much higher than in the nonallergic, normal, or unsensitized control mice. Tissue eosinophilia and intestinal ECP levels were significantly correlated with the RANTES mRNA level. We conclude that RANTES may play a central role in the pathogenesis of food-mediated gastrointestinal allergy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1081/imm-120027682DOI Listing
February 2004

Immunization with Trichinella spiralis Korean isolate larval excretory-secretory antigen induces protection and lymphocyte subset changes in rats.

Immunol Invest 2004 Feb;33(1):15-26

The Institute for Tropical Endemic Diseases, Korea University College of Medicine, Seoul, Korea.

Protection and immune responses were studied in rats immunized with Trichinella spiralis muscle stage larval excretory-secretory antigen (ES Ag) without adjuvant. Protection was assessed by the degree of adult worm burden and the yield of muscle (diaphragmatic) larvae after challenge infection with live larvae. Lymphocyte subsets were identified by flow cytometry in the spleen and peripheral blood. Cytokine production and specific IgG, IgG1 and IgG2a antibody responses were measured. Immunization with ES Ag produced highly significant protection against adult stages (98.4%) and muscle larvae (82.9%). Th2 type cytokines (IL-10, IL-4) were predominant. Anti-muscle stage larval ES Ag antibody was significantly elevated in the order IgG2a > IgG1 > IgG on the 2nd day after final immunization and on the 7th day after challenge infection. Expression of CD4+ and the CD4+/CD8+ ratio from spleen and blood were significantly increased compared to the control. These results demonstrate that immunization with T. spiralis antigen can elicit robust immune response, resulting in complete protection against infective larvae, and this protection can be achieved without the use of any adjuvant.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1081/imm-120027681DOI Listing
February 2004
-->