Publications by authors named "Timm T Ensfelder"

2 Publications

  • Page 1 of 1

Synthesis of Galactosyl-Queuosine and Distribution of Hypermodified Q-Nucleosides in Mouse Tissues.

Angew Chem Int Ed Engl 2020 07 21;59(30):12352-12356. Epub 2020 Apr 21.

Department of Chemistry, Ludwig-Maximilians-Universität München, Butenandtstr. 5-13, 81377, Munich, Germany.

Queuosine (Q) is a hypermodified RNA nucleoside that is found in tRNA , tRNA , tRNA , and tRNA . It is located at the wobble position of the tRNA anticodon loop, where it can interact with U as well as C bases located at the respective position of the corresponding mRNA codons. In tRNA and tRNA of higher eukaryotes, including humans, the Q base is for yet unknown reasons further modified by the addition of a galactose and a mannose sugar, respectively. The reason for this additional modification, and how the sugar modification is orchestrated with Q formation and insertion, is unknown. Here, we report a total synthesis of the hypermodified nucleoside galactosyl-queuosine (galQ). The availability of the compound enabled us to study the absolute levels of the Q-family nucleosides in six different organs of newborn and adult mice, and also in human cytosolic tRNA. Our synthesis now paves the way to a more detailed analysis of the biological function of the Q-nucleoside family.
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http://dx.doi.org/10.1002/anie.202002295DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7384130PMC
July 2020

ALKBH5-induced demethylation of mono- and dimethylated adenosine.

Chem Commun (Camb) 2018 Aug 16;54(62):8591-8593. Epub 2018 Jul 16.

Center for Integrated Protein Science, Department of Chemistry, Ludwig-Maximilians-Universität München, Butenandtstraße 5-13, 81377 Munich, Germany.

RNA contains methylated A-base derivatives. A methylation to mA and then demethylation regulate homeostasis in mRNA. It is assumed that mA is mainly demethylated by the α-ketoglutarate dependent oxidase ALKBH5. Here we show that ALKBH5 also demethylates the dimethylated adenosine mA, which is a non-canonical base present in ribosomal RNA.
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http://dx.doi.org/10.1039/c8cc03980aDOI Listing
August 2018