Publications by authors named "Tianze Wang"

12 Publications

  • Page 1 of 1

Selenium fertigation with nanobubbles influences soil selenium residual and plant performance by modulation of bacterial community.

J Hazard Mater 2021 Sep 3;423(Pt B):127114. Epub 2021 Sep 3.

College of Water Resources and Civil Engineering, China Agricultural University, Beijing 100083, China; Engineering Research Center for Agricultural Water-Saving and Water Resources, Ministry of Education, China Agricultural University, Beijing 100083, China. Electronic address:

Although selenium (Se) is an essential microelement for humans and animals, it is a potentially toxic element due to its bioaccumulation potential. In this study, Se fertilizer was supplied in a greenhouse vegetable (cucumber) plantation using an innovative system consisting of nanobubbles (NB_Se) and compared to that under conventional conditions of fertigation (C_Se) with six doses. The results revealed that NB_Se significantly reduced soil Se accumulation (38%-144%) and increased cucumber Se content compared with the C_Se treatments at the same Se dose. NB_Se significantly lowered the soil bacterial diversity, with an initial increase and then decrease with the Se doses. Bacterial associations and potential keystone taxa also differed between the NB_Se and C_Se. The greater abundance of oxidizing bacteria (indicated by the function composition of bacterial community) and the improved soil redox environment created by NBs sustained more available Se for plants, leading to a reduction in soil Se residual and an increase in the plant Se content. Our results highlight the feasibility and efficiency of NB_Se and demonstrate the important implications of Se for the maintenance of soil health and sustainability.
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http://dx.doi.org/10.1016/j.jhazmat.2021.127114DOI Listing
September 2021

Pyruvate dehydrogenase deficiency disease detected by the enzyme activity of peripheral leukocytes.

Mol Genet Genomic Med 2021 Aug 22;9(8):e1728. Epub 2021 Jun 22.

ChinovoLaboratory, Beijing, P. R. China.

Background: Pyruvate dehydrogenase complex (PDHC) deficiency is a common neurodegenerative disease associated with abnormal mitochondrial energy metabolism. The diagnosis of PDHC is difficult because of the lack of a rapid, accurate, and cost-effective clinical diagnostic method.

Methods: A 4-year-old boy was preliminarily diagnosed with putative Leigh syndrome based on the clinical presentation. PDHC activity in peripheral blood leukocytes and a corresponding gene analysis were subsequently undertaken. Sodium pyruvate 1- C was used for the analysis of PDHC activity in peripheral leukocytes. The genes encoding PDHC were then scanned for mutations.

Results: The results showed that the corresponding PDHC activity was dramatically decreased to 10.5 nmol/h/mg protein as compared with that of healthy controls (124.6 ± 7.1 nmol/h/mg). The ratio of PDHC to citrate synthase was 2.1% (control: 425.3 ± 27.1). The mutation analysis led to the identification of a missense mutation, NM_000284.4:g214C>T, in exon 3 of PDHC.

Conclusion: The peripheral blood leukocyte PDHC activity assay may provide a practical enzymatic diagnostic method for PDHC-related mitochondrial diseases.
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http://dx.doi.org/10.1002/mgg3.1728DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8404224PMC
August 2021

In which case is working memory for movements affected by verbal interference? Evidence from the verbal description of movement.

Memory 2021 07 21;29(6):762-777. Epub 2021 Jun 21.

Faculty of Education, Guangxi Normal University, Guilin, People's Republic of China.

Current perspectives on whether verbal interference affects working memory for movements have not yet reached a consensus. This study explored the causes of this controversy to reveal the relation between working memory for movements and the phonological loop. Experiment 1 explored whether the verbal description of movement moderated the effect of verbal interference (articulatory suppression) on working memory for movements. Verbal interference only affected working memory for easy-to-describe movements (lower accuracy). Experiment 2 excluded the interpretation of familiarity to the controversy and the effect of familiarity on the results of Experiment 1. Experiment 3 verified the results of Experiment 1 with another form of verbal interference, i.e., presenting irrelevant words visually. These three experiments suggest that the phonological loop is not recruited for processing working memory for movements in nature, but the two may interact through the verbal description prestored in the long-term memory. Thus, the current study provides a certain level of support for the separable movement-based subsystem hypothesis (Smyth, M. M., Pearson, N. A., & Pendleton, L. R. (1988). Movement and working memory: Patterns and positions in space. (3), 497-514. doi:10.1080/02724988843000041).
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http://dx.doi.org/10.1080/09658211.2021.1944217DOI Listing
July 2021

Fluorometric determination of the breast cancer 1 gene based on the target-induced conformational change of a DNA template for copper nanoclusters.

Anal Methods 2021 02 22;13(5):712-718. Epub 2021 Jan 22.

School of Materials Science and Engineering, Xiangtan University, Xiangtan 411105, PR China.

The breast cancer 1 (BRCA1) gene is a tumor suppressor gene, whose mutation is closely related to breast cancer. Therefore, the sensitive detection of the BRCA1 gene is extremely important for human health, particularly for women. In this study, a label-free fluorescent method based on hairpin DNA-templated copper nanoclusters (CuNCs) was for the first time developed for the detection of the BRCA1 gene. In the absence of target DNA, the detection system showed a strong red emission and produced a high emission peak. However, in the presence of the BRCA1 gene, the DNA probe hybridized with the BRCA1 gene and conformation of the DNA probe changed. As a result, the amount of produced CuNCs decreased and a low emission peak was obtained. The fluorescence intensity of the detection system was linearly correlated with the concentration of the BRCA1 gene ranging from 2 nM to 600 nM. The detectable limit was 2 nM for the BRCA1 gene assay, which was comparable with those reported by other non-amplifying sensors. Moreover, the developed method showed satisfactory recoveries for the BRCA1 gene assay in the bovine serum. The DNA-templated CuNC-based fluorescent assay thus offered a promising platform for the diagnosis of a breast cancer biomarker.
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http://dx.doi.org/10.1039/d0ay01712dDOI Listing
February 2021

Orderliness/Disorderliness Is Mentally Associated With Construal Level and Psychological Distance.

Front Psychol 2019 20;10:2521. Epub 2019 Nov 20.

School of Education and Psychology, University of Jinan, Jinan, China.

People are innately capable of exploring and detecting orderliness and of attempting to make the world in which they live more orderly rather than more disorderly. Construal level theory asserts that the same stimuli can be represented abstractly or concretely and that psychological distance can affect the construal level. No research, however, has examined whether perceived orderliness/disorderliness is mentally associated with construal level and psychological distance. In this study, by using the Implicit Association Test (IAT), we conducted 10 studies to examine this possibility. The results of studies 1A-1B showed that people tended to associate high-level construal concepts with orderliness concepts and low-level construal concepts with disorderliness concepts. By contrast, the results of studies 2A-5B revealed that people associated psychologically proximal concepts with orderliness concepts and psychologically distal concepts with disorderliness concepts. These studies demonstrated that orderliness/disorderliness is associated with both construal level and psychological distance, but in opposite directions, suggesting that construal level and psychological distance may have distinct natures.
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http://dx.doi.org/10.3389/fpsyg.2019.02521DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6880731PMC
November 2019

Characterization of initial reaction intermediates in heated model systems of glucose, glutathione, and aliphatic aldehydes.

Food Chem 2020 Feb 5;305:125482. Epub 2019 Sep 5.

Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University (BTBU), Beijing 100048, China.

To understand the effect of lipid degradation on Maillard formation of meaty flavors, initial reaction intermediates in model systems of glucose-glutathione with hexanal, (E)-2-heptenal, or (E,E)-2,4-decadienal were identified by HPLC-MS and by NMR. Besides Amadori compounds, hemiacetals and thiazolidines via addition of sulfhydryl to carbonyl or to the conjugated olefinic bond were found. Concentrations of all intermediates increased with reaction time while degradation of the intermediates with a glutathione moiety helped formation of thiazolidines with cysteinylglycine. The unsaturated aldehydes (E)-2-heptenal and (E,E)-2,4-decadienal exhibited high reactivity against glucose for glutathione, yielding higher levels of intermediate compounds than from glucose. Heating prepared intermediates reversibly released the original aldehydes, which caused various compounds formed by retro-aldol, oxidation, etc. to react with HS and NH. Among them, formation pathways including 3-nonen-2-one, 2-hexanoylfuran, and six dialkylthiophenes (e.g., 2-ethyl-5-(1-methylbutyl)thiophene) were proposed for the first time.
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http://dx.doi.org/10.1016/j.foodchem.2019.125482DOI Listing
February 2020

Heteroplasmy Detection of Mitochondrial DNA A3243G Mutation Using Quantitative Real-Time PCR Assay Based on TaqMan-MGB Probes.

Biomed Res Int 2018 13;2018:1286480. Epub 2018 Nov 13.

Chinovo Laboratory, Beijing, China.

A point mutation of mitochondrial DNA (mtDNA) at nucleotide position 3243 A to G (mt.3243A>G) is involved in many common diseases, including maternally inherited diabetes and deafness (MIDD) and mitochondrial encephalomyopathy, lactic acidosis with stroke-like episodes (MELAS). However, the mutant level of mt.3243A>G varies both among individuals and in different organs, tissues, and even cells of single individuals. For detection of this mutation, current methods have limited universality and sensitivity and may be not adequate for a routine clinical test. Here, we develop and evaluate a rapid TaqMan-MGB quantitative real-time PCR (qPCR) method for detecting and quantifying the heteroplasmy level of mt.3243A>G in single-tube analysis. With our method, the sensitivity of detection was as low as 0.1%, but the accuracy of quantification was reliable, down to 4%. All positives could be correctly identified, and the heteroplasmy levels determined by qPCR correlated well with the results from restriction fragment length polymorphism (RFLP) and pyrosequencing assays (r = 0.921~0.973 and 0.972~0.984). In addition, we demonstrated that the urinary sediments, leukocytes, or hair follicles might be ideal templates to detect and quantify the heteroplasmy of mt.3243A>G mutation; however, they should be optimized or retreated for further accurate quantification. Our study should allow rapid and high throughput diagnostic testing and can potentially be used to clarify the association between clinical phenotype and pathogenic mitochondrial mutations derived from various tissues.
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http://dx.doi.org/10.1155/2018/1286480DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6260548PMC
March 2019

Meat flavor generation from different composition patterns of initial Maillard stage intermediates formed in heated cysteine-xylose-glycine reaction systems.

Food Chem 2019 Feb 23;274:79-88. Epub 2018 Aug 23.

Institute of Quality Standard and Testing Technology for Agro-products of CAAS, Beijing 100081, China.

Volatile compounds formed in model reactions involving synthesized initial Maillard intermediates Gly-Amadori and [C]-2-threityl-thiazolidine-4-carboxylic acids ([C]-TTCA) in different molar ratios and free cysteine and glycine were investigated by solid-phase microextraction/gas chromatography-mass spectrometry and gas chromatography-olfactometry. The 1:1 ratio composition pattern provided the highest yields of all the sulfur-containing compounds, the potent meaty flavors or their C-labeled/unlabeled fractions, indicating a moderate level of glycine relative to cysteine was optimum for maximally yielding meaty flavors in complex meat-like Maillard systems containing cysteine as well as glycine. In addition, the 1:1 ratio composition led to formation of C-labeled molecules of some key meaty flavors e.g. 2-furanthiol representing over 70%, indicating TTCA/glycine reaction was better than Gly-Amadori/cysteine to yield meaty flavors. Formation pathways of twenty-nine flavors were elucidated based on the detected isotope distribution patterns. In particular, 2-methyltetrahydrothiophen-3-one, 3-thiophenethiol, 2-ethylthiophene, 2,5-dimethylthiophene, and 5-methylthiophene-2-carboxaldehyde involved a new formation pathway. Thiophene-2-carboxaldehyde and 2-methylthieno[3,2-b]thiophene showed two formation pathways.
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http://dx.doi.org/10.1016/j.foodchem.2018.08.096DOI Listing
February 2019

Aroma Compounds in Chicken Broths of Beijing Youji and Commercial Broilers.

J Agric Food Chem 2018 Oct 21;66(39):10242-10251. Epub 2018 Sep 21.

Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Laboratory for Food Quality and Safety, and Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients , Beijing Technology and Business University (BTBU) , Beijing 100048 , China.

The unique flavor of Beijing Youji (BJY) chicken broth compared with that of commercial broilers (CB) was investigated by solvent-assisted flavor evaporation combined with AEDA/GC-O (aroma extract dilution analysis of gas chromatography-olfactometry), quantitation, and aroma recombination. A total of 71 odorants with almost the same major odorants (≥10 ng/g broth) were found by GC-O in both BJY and CB broths. However, BJY broth had thirty-two more extra odorants than CB broth, indicating the rich fragrance of the former. Aroma recombination and omission experiments demonstrated that 21 versus 17 odorants (with OAV ≥ 1) contributed significantly to BJY and CB broth aromas, respectively. Those key odorants mainly included sulfur-containing compounds and aliphatic aldehydes, such as 2-methyl-3-furanthiol, 3-(methylthio)propanal, ( E, E)-2,4-decadienal, etc. Furthermore, composition analysis of the meat suggested that the better flavor, with rather more odorants, of BJY broth is probably due to higher contents of polyunsaturated fatty acids and water-soluble flavor precursor, including ribose, cysteine, thiamine, etc., present in the BJY meat.
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http://dx.doi.org/10.1021/acs.jafc.8b03297DOI Listing
October 2018

Formation mechanism of aroma compounds in a glutathione-glucose reaction with fat or oxidized fat.

Food Chem 2019 Jan 18;270:436-444. Epub 2018 Jul 18.

Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Laboratory for Food Quality and Safety, Beijing Technology and Business University (BTBU), Beijing 100048, China.

Glutathione and glucose with or without chicken fat/oxidized chicken fat were thermally reacted for generation of stewed meat-like aroma, where 42 sulfur-containing odorants were identified by gas chromatography-mass spectrometry (GC-MS) and gas chromatography-olfactometry (GC-O). The observed effects or interactions on meat flavor formation due to the fats were similar to previous reports of cysteine-reducing sugar reactions. Carbohydrate module labeling approach demonstrated ten alkyl chain compounds were indeed resulted from the lipid degradation-Maillard reaction interactions, whereas the fats had little effect on formation pathways of compounds only derived from the Maillard reaction. Formation pathways of 26 potent aroma compounds were proposed, particularly, involving two benzene derivatives and seven complex thiophenes. Notably, it was found for the first time just 2-ethylthiophene could result from both an intact skeleton of glucose and the lipid degradation product of 2,4-hexadienal, and the carbohydrate modules methylglyoxal and hydroxyacetone could arise from the glutamic acid of GSH.
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http://dx.doi.org/10.1016/j.foodchem.2018.07.106DOI Listing
January 2019

Negatively Regulates the Suppressive Function of Myeloid-Derived Suppressor Cells.

Cancer Immunol Res 2018 11 31;6(11):1352-1363. Epub 2018 Aug 31.

State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, China.

Myeloid-derived suppressor cells (MDSC) are regulators of immune responses in cancer. The differentiation and function of these MDSCs may be regulated through multiple factors, such as microRNAs. However, the effect of long noncoding RNAs (lncRNA) on the differentiation and function of MDSCs is poorly understood. We identified a long noncoding RNA (lncRNA) named in MDSCs, which may control suppressive functions of MDSCs. could be induced in and tumor and inflammatory environments. It regulated a set of target transcripts, such as Arg-1, NOS2, NOX2, and COX2, to control immune-suppressive function and differentiation of MDSCs. This lncRNA was also able to bind to the C/EBPβ isoform LIP to inhibit the activation of C/EBPβ. We also found that the conserved homologue has a similar function to murine These findings suggest a negative feedback role for in controlling the immunosuppressive functions of MDSC in the tumor environment. .
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http://dx.doi.org/10.1158/2326-6066.CIR-18-0108DOI Listing
November 2018

Gut REG3γ-Associated Induces Anti-inflammatory Macrophages to Maintain Adipose Tissue Homeostasis.

Front Immunol 2017 4;8:1063. Epub 2017 Sep 4.

State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, China.

Gut microbiota may not only affect composition of local immune cells but also affect systemic immune cells. However, it is not completely clear how gut microbiota modulate these immune systems. Here, we found that there exist expanded macrophage pools in γ mice. REG3γ-associated , which is homology to , could enlarge macrophage pools not only in the small intestinal lamina propria but also in the spleen and adipose tissues. STAT3-mediated signal(s) was a critical factor in the -mediated anti-inflammatory macrophages. We also offered evidence for critical cellular links among REG3γ-associated , tissue macrophages, and obesity diseases. Anti-inflammatory macrophages in the lamina propria, which are induced by REG3γ-associated , may migrate into adipose tissues and are involved in resistance against high-fat diet-mediated obesity. Thus, REG3γ-associated -induced anti-inflammatory macrophages in gut tissues may play a role in adipose tissue homeostasis.
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http://dx.doi.org/10.3389/fimmu.2017.01063DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5591335PMC
September 2017
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