Publications by authors named "Tianyu Chen"

71 Publications

Co-Delivery of 5-Fluorouracil and Paclitaxel in Mitochondria-Targeted KLA-Modified Liposomes to Improve Triple-Negative Breast Cancer Treatment.

Pharmaceuticals (Basel) 2022 Jul 17;15(7). Epub 2022 Jul 17.

School of Pharmacy, Chengdu Medical College, No. 783 Xindu Avenue, Xindu District, Chengdu 610500, China.

In this research, KLA-modified liposomes co-loaded with 5-fluorouracil and paclitaxel (KLA-5-FU/PTX Lps) were developed, and their antitumor activity against triple-negative breast cancer (TNBC) was evaluated. KLA-5-FU/PTX Lps were prepared using the thin-film dispersion method, and their in vitro anticancer efficacy was assessed in human breast cancer cells (MDA-MB-231). An MDA-MB-231 tumor-bearing mouse model was also established to evaluate their antitumor efficacy in vivo. KLA-5-FU/PTX Lps showed enhanced cytotoxicity against MDA-MB-231 cells, improved drug delivery to mitochondria, and induced mitochondria-mediated apoptosis. The modified liposomes also showed favorable antitumor activity in vivo due to their strong ability to target tumors and mitochondria. The liposomes showed no obvious systemic toxicity. Our results suggest that KLA-5-FU/PTX Lps are a promising system with which to target the delivery of antitumor drugs to mitochondria as a treatment for TNBC.
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http://dx.doi.org/10.3390/ph15070881DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318860PMC
July 2022

Assessing the Biodegradation of BTEX and Stress Response in a Bio-Permeable Reactive Barrier Using Compound-Specific Isotope Analysis.

Int J Environ Res Public Health 2022 Jul 20;19(14). Epub 2022 Jul 20.

State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, China.

By using compound-specific isotope analysis (CSIA) in combination with high-throughput sequencing analysis (HTS), we successfully evaluated the benzene and toluene biodegradation in a bio-permeable reactive barrier (bio-PRB) and the stress response of the microbial community. Under stress conditions, a greater decline in the biodegradation rate of BTEX was observed compared with the apparent removal rate. Both an increase in the influent concentration and the addition of trichloroethylene (TCE) inhibited benzene biodegradation, while toluene biodegradation was inhibited by TCE. Regarding the stress response, the relative abundance of the dominant bacterial community responsible for the biodegradation of BTEX increased with the influent concentration. However, the dominant bacterial community did not change, and its relative abundance was restored after the influent concentration decreased. On the contrary, the addition of TCE significantly changed the bacterial community, with becoming the dominant phyla for co-metabolizing TCE and BTEX. Thus, TCE had a more significant influence on the bio-PRB than an increasing influent concentration, although these two stress conditions showed a similar degree of influence on the apparent removal rate of benzene and toluene. The present work not only provides a new method for accurately evaluating the biodegradation performance and microbial community in a bio-PRB, but also expands the application of compound-specific isotope analysis in the biological treatment of wastewater.
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http://dx.doi.org/10.3390/ijerph19148800DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322891PMC
July 2022

Discovery of Tropolone Stipitaldehyde as a Potential Agent for Controlling Phytophthora Blight and Its Action Mechanism Research.

J Agric Food Chem 2022 Jul 6;70(28):8693-8703. Epub 2022 Jul 6.

College of Plant Protection, State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing Agricultural University, Nanjing 210095, P. R. China.

The fermentation of endophytic GGY-3 resulted in the isolation of tropolone stipitaldehyde (), which exhibited broad-spectrum inhibition activity against fungi and bacteria, especially against , with an EC value of 0.83 μg/mL and pv. , with a minimum inhibitory concentration value of 4.0 μg/mL. The in vitro and in vivo assays demonstrated that had a significant protective effect on . Furthermore, inhibited the spore germination of and damaged the plasma membrane structure. As observed by SEM and TEM, after exposure to , mycelia exhibited swelling, shrunken, branch-increasing phenomena, cell wall and membrane damage, and disordered content. Transcriptome analysis revealed that might affect starch and sucrose metabolism and fatty acid biosynthesis by suppressing the expression of genes relevant to cell wall synthetases and cell membrane-associated genes. These findings indicate that may be a potential agrochemical fungicide for controlling phytophthora blight.
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http://dx.doi.org/10.1021/acs.jafc.2c03163DOI Listing
July 2022

Federated Learning Empowered Real-Time Medical Data Processing Method for Smart Healthcare.

IEEE/ACM Trans Comput Biol Bioinform 2022 Jun 23;PP. Epub 2022 Jun 23.

Computer-aided diagnosis (CAD) has always been an important research topic for applying artificial intelligence in smart healthcare. Sufficient medical data are one of the most critical factors in CAD research. However, medical data are usually obtained in chronological order and cannot be collected all at once, which poses difficulties for the application of deep learning technology in the medical field. The traditional batch learning method consumes considerable time and space resources for real-time medical data, and the incremental learning method often leads to catastrophic forgetting. To solve these problems, we propose a real-time medical data processing method based on federated learning. We divide the process into the model stage and the exemplar stage. In the model stage, we use the federated learning method to fuse the old and new models to mitigate the catastrophic forgetting problem of the new model. In the exemplar stage, we use the most representative exemplars selected from the old data to help the new model review the old knowledge, which further mitigates the catastrophic forgetting problem of the new model. We use this method to conduct experiments on a simulated medical real-time data stream. The experimental results show that our method can learn a disease diagnosis model from a continuous medical real-time data stream. As the amount of data increases, the performance of the disease diagnosis model continues to improve, and the catastrophic forgetting problem has been effectively mitigated. Compared with the traditional batch learning method, our method can significantly save time and space resources.
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http://dx.doi.org/10.1109/TCBB.2022.3185395DOI Listing
June 2022

Mutation of rpoS is Beneficial for Suppressing Organic Acid Secretion During 1,3-Propandiol Biosynthesis in Klebsiella pneumoniae.

Curr Microbiol 2022 Jun 15;79(8):218. Epub 2022 Jun 15.

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, People's Republic of China.

In this study, to reduce the formation of organic acid during 1,3-propanediol biosynthesis in Klebsiella pneumoniae, a method combining UV mutagenesis and high-throughput screening with pH color plates was employed to obtain K. pneumoniae mutants. When compared with the parent strain, the total organic acid formation by the mutant decreased, whereas 1,3-propanediol biosynthesis increased after 24 h anaerobic shake flask culture. Subsequently, genetic changes in the mutant were analyzed by whole-genome sequencing and verified by signal gene deletion. Mutation of the rpoS gene was confirmed to contribute to the regulation of organic acid synthesis in K. pneumoniae. Besides, rpoS deletion eliminated the formation of 2,3-butanediol, the main byproduct produced during 1,3-propanediol fermentation, indicating the role of rpoS in metabolic regulation in K. pneumoniae. Thus, a K. pneumoniae mutant was developed, which could produce lower organic acid during 1,3-propanediol fermentation due to an rpoS mutation in this study.
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http://dx.doi.org/10.1007/s00284-022-02901-wDOI Listing
June 2022

A Cantilever Beam-Based Triboelectric Nanogenerator as a Drill Pipe Transverse Vibration Energy Harvester Powering Intelligent Exploitation System.

Sensors (Basel) 2022 Jun 4;22(11). Epub 2022 Jun 4.

Dalian Key Lab of Marine Micro/Nano Energy and Self-Powered Systems, Marine Engineering College, Dalian Maritime University, Dalian 116026, China.

Measurement While Drilling (MWD) is the most commonly used real-time information acquisition technique in offshore intelligent drilling, its power supply has always been a concern. Triboelectric nanogenerators have been shown to harvest low-frequency vibrational energy in the environment and convert it into electricity to power small sensors and electrical devices. This work proposed a cantilever-beam-based triboelectric nanogenerator (CB-TENG) for transverse vibration energy harvesting of a drill pipe. The CB-TENG consists of two vibrators composed of spring steel with PTFE attached and Al electrodes. The structurally optimized CB-TENG can output a peak power of 2.56 mW under the vibration condition of = 3.0 Hz and = 50 mm, and the electrical output can be further enhanced with the increased vibration parameters. An array-type vibration energy harvester integrated with eight CB-TENGs is designed to fully adapt to the interior of the drill pipe and improve output performance. The device can realize omnidirectional vibration energy harvesting in the two-dimensional plane with good robustness. Under the typical vibration condition, the short-circuit current and the peak power can reach 49.85 μA and 30.95 mW, respectively. Finally, a series of demonstration experiments have been carried out, indicating the application prospects of the device.
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http://dx.doi.org/10.3390/s22114287DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9185564PMC
June 2022

MRI-based Texture Analysis of Infrapatellar Fat Pad to Predict Knee Osteoarthritis Incidence.

Radiology 2022 May 31:212009. Epub 2022 May 31.

From the Clinical Research Centre, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China (J.L., Z.Z., P.C., T.C., X.W., D.J.H., C.D.); Department of Orthopedics, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China (J.L.); School of Biomedical Engineering, Southern Medical University, Guangzhou, China (S.F., D.Z., J.M.); Department of Rehabilitation Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, China (Z.G.); School of Rehabilitation Medicine, Southern Medical University, Guangzhou, China (Z.G.); Department of Radiology, Zhujiang Hospital, Southern Medical University, Guangzhou, China (T.L.); Department of Orthopedics, The Third Affiliated Hospital of Southern Medical University, Guangzhou, China (T.C.); Department of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio (R.L.); University of Arizona College of Medicine, Tucson, Ariz (C.K.K.); University of Pittsburgh Graduate School of Public Health, Pittsburgh, Pa (C.K.K.); Department of Radiology, VA Boston Healthcare System, Boston University School of Medicine, Boston, Mass (A.G., F.W.R.); Department of Radiology, University of Erlangen-Nuremberg, Erlangen, Germany (F.W.R.); Department of Rheumatology, Royal North Shore Hospital and Sydney Musculoskeletal Health, Kolling Institute, University of Sydney, Australia (D.J.H.); and Menzies Institute for Medical Research, University of Tasmania, Hobart, Australia (C.D.).

Background Infrapatellar fat pad (IPFP) quality has been implicated as a marker for predicting knee osteoarthritis (KOA); however, no valid quantification for subtle IPFP abnormalities has been established. Purpose The purpose of this study was to investigate whether MRI-based three-dimensional texture analysis of IPFP abnormalities could help predict incident radiographic KOA. Materials and Methods In this prospective nested case-control study, 690 participants whose knees were at risk for KOA were included from the Pivotal Osteoarthritis Initiative MRI Analyses incident osteoarthritis cohort. All knees had a Kellgren-Lawrence grade of 1 or less at baseline. During the 4-year follow-up, case participants were matched 1:1 to control participants, with incident radiographic KOA as the outcome. MRI scans were segmented at the incident time point of KOA (hereafter, P0), 1 year before P0 (hereafter, P-1), and baseline. MRI-based three-dimensional texture analysis was performed to extract IPFP texture features. Least absolute shrinkage and selection operator and multivariable logistic regressions were applied in the development cohort and evaluated in the test cohort. The area under the receiver operating characteristic curve (AUC) was used to evaluate the discriminative value of the clinical score, IPFP texture score, and MRI Osteoarthritis Knee Score. Results Participants were allocated to development ( = 500, 340 women; mean age, 60 years) and test ( = 190, 120 women; mean age, 61 years) cohorts. In both cohorts, IPFP texture scores (AUC ≥0.75 for all) showed greater discrimination than clinical scores (AUC ≤0.69 for all) at baseline, P-1, and P0, with significant differences in pairwise comparisons ( ≤ .002 for all). Greater predictive and concurrent validities of IPFP texture scores (AUC ≥0.75 for all) compared with MRI Osteoarthritis Knee Scores (AUC ≤0.66 for all) were also demonstrated ( < .001 for all). Conclusion MRI-based three-dimensional texture of the infrapatellar fat pad was associated with future development of knee osteoarthritis. ClinicalTrials.gov registration no.: NCT00080171 © RSNA, 2022 See also the editorial by Fischer in this issue.
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http://dx.doi.org/10.1148/radiol.212009DOI Listing
May 2022

Engineering Multifunctional Hydrogel With Osteogenic Capacity for Critical-Size Segmental Bone Defect Repair.

Front Bioeng Biotechnol 2022 9;10:899457. Epub 2022 May 9.

Department of Orthopaedic, Huizhou First Hospital, Guangdong Medical University, Huizhou, China.

Treating critical-size segmental bone defects is an arduous challenge in clinical work. Preparation of bone graft substitutes with notable osteoinductive properties is a feasible strategy for critical-size bone defects. Herein, a biocompatible hydrogel was designed by dynamic supramolecular assembly of polyvinyl alcohol (PVA), sodium tetraborate (NaBO), and tetraethyl orthosilicate (TEOS). The characteristics of the supramolecular hydrogel were evaluated by rheological analysis, swelling ratio, degradation experiments, and scanning electron microscopy (SEM). In experiments, this TEOS-hydrogel had self-healing property, low swelling rate, degradability, good biocompatibility, and induced osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) by upregulating the expression of Runx-2, Col-1, OCN, and osteopontin (OPN). In segmental bone defect rabbit models, the TEOS-containing hydrogel accelerated bone regeneration, thus restoring the continuity of bone and recanalization of the medullary cavity. The abovementioned results demonstrated that this TEOS-hydrogel has the potential to realize bone healing in critical-size segmental bone defects.
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http://dx.doi.org/10.3389/fbioe.2022.899457DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9124794PMC
May 2022

Determination of Picogram-per-Gram Concentrations of Pa and Th in Sediments by Melt Quenching and Laser Ablation Mass Spectrometry.

Anal Chem 2022 05 16;94(21):7576-7583. Epub 2022 May 16.

Laboratory for Marine Geology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China.

Uranium, thorium, and protactinium radionuclides in marine sediments are important proxies for understanding the earth's environmental evolution. Conventional solution-based methods, which typically involve isotope spike preparation, concentrated acid sample digestion, column chemistry, and mass spectrometry, allow precise but time-consuming and costly measurements of these nuclide concentrations (i.e., Th and Pa). In this work, we have established an efficient method for Th and Pa measurement of marine sediments down to the picogram-per-gram level without purification and enrichment. Our method first transforms a small amount of thermally decomposed sediments (∼0.1-0.2 g) to homogeneous silicate glass using a melt quenching technique and then analyzes the glass with laser ablation multicollector inductively coupled plasma-mass spectrometry. Standard sample bracketing with isotope-spike-calibrated glass standards prepared in this study was used to correct for instrumental fractionation during measurement. It is demonstrated that our method can accurately determine the U-Th-Pa concentrations of typical marine sediments in the late Pleistocene with precision of a few percent. Compared with the conventional solution-based methods, the turnover time of sample preparation and measurement with our established protocol is greatly reduced, facilitating future application of U-series radionuclides in reconstructing oceanic processes at high temporal and spatial resolution.
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http://dx.doi.org/10.1021/acs.analchem.2c00438DOI Listing
May 2022

A Novel 3D Visualized Operative Procedure in the Single-Stage Complete Repair With Unifocalization of Pulmonary Atresia With Ventricular Septal Defect and Major Aortopulmonary Collateral Arteries.

Front Cardiovasc Med 2022 25;9:836200. Epub 2022 Apr 25.

Department of Cardiovascular Surgery, Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.

Objectives: Pulmonary atresia with ventricular septal defect and major aortopulmonary collateral arteries (PA/VSD/MAPCAs) is a relatively rare, complex, and heterogeneous congenital heart disease. As one of the effective treatments, the midline unifocalization strategy still remains complicated and challenging due to the diverse forms of MAPCAs and pulmonary arteries. The purpose of this study is to summarize our experience of a novel three-dimensional (3D) visualized operative procedure in the single-stage complete repair with unifocalization and to clarify the benefits it may bring to us.

Methods: We described our experience of the 3D visualized operative procedure such as 3D printing, virtual reality (VR), and mixed reality (MR) technology in patients with PA/VSD/MAPCAs who underwent a single-stage complete repair with unifocalization. The data from the patients who underwent this procedure (3D group) and those who underwent the conventional procedure (conventional group) were compared.

Results: The conventional and 3D groups included 11 patients from September 2011 to December 2017 and 9 from January 2018 to March 2021, respectively. The baseline characteristics such as age, body weight, preoperative saturation, the anatomy of the pulmonary arteries and MAPCAs, the Nakata index, and TNPAI had no statistical significance. All 9 patients in the 3D group were operated only through a median sternotomy, while 8 cases (72.7%) in the conventional group needed another posterolateral thoracotomy ( = 0.001). In the 3D group, the CPB time was shorter (93.2 ± 63.8 vs. 145.1 ± 68.4 min, = 0.099), and the median pre-CPB time per MAPCAs was significantly shorter [25.7 (14.0, 46.3) vs. 65 (41.3, 75.0) min, = 0.031]. There was no early death in the 3D group, while there were 3 in the conventional group (0 vs. 27.3%, = 0.218).

Conclusion: The novel 3D visualized operative procedure may help improve the performance of the single-stage complete repair with the midline unifocalization of PA/VSD/MAPCAs and help shorten the dissecting time of the MAPCAs. It may promote the routine and successful application of this strategy in more centers.
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http://dx.doi.org/10.3389/fcvm.2022.836200DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9081567PMC
April 2022

Role of Selenium and Vitamins E and B9 in the Alleviation of Bovine Mastitis during the Periparturient Period.

Antioxidants (Basel) 2022 Mar 29;11(4). Epub 2022 Mar 29.

Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control, State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.

Mastitis (inflammation of the mammary gland) commonly occurs in dairy cattle during the periparturient period (transition period), in which dairy cattle experience physiological and hormonal changes and severe negative energy balance, followed by oxidative stress. To maintain successful lactation and combat negative energy balance (NEB), excessive fat mobilization occurs, leading to overproduction of reactive oxygen species (ROS). Excessive fat mobilization also increases the concentrations of nonesterified fatty acids (NEFA) and β-hydroxybutyric acid (BHB) during the periparturient period. In addition, the excessive utilization of oxygen by cellular respiration in the mammary causes abnormal production of oxidative stress (OS). OS impairs the immunity and anti-inflammatory efficiency of periparturient dairy cattle, increasing their susceptibility to mastitis. To alleviate oxidative stress and subsequent mastitis, antioxidants are supplemented to dairy cattle from an external source. Extensive studies have been conducted on the supplementation of selenium (Se) and vitamins E and B9 to mitigate mastitis during the transition period in dairy cattle. Altogether, in the current review, we discuss the research development on bovine mastitis and its major causes, with special emphasis on oxidative stress during the transition period. Moreover, we discuss the antioxidant, immunoregulatory, and anti-inflammatory properties of Se and vitamins E and B9 and their role in the control of bovine mastitis in periparturient dairy cattle.
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http://dx.doi.org/10.3390/antiox11040657DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9032172PMC
March 2022

Arthroscopic shoulder release in the treatment of frozen shoulder: A case series.

Asian J Surg 2022 08 8;45(8):1652-1653. Epub 2022 Apr 8.

Department of Orthopaedics, Neijiang Hospital of Traditional Chinese Medicine, Neijiang, 641000, China.

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http://dx.doi.org/10.1016/j.asjsur.2022.03.077DOI Listing
August 2022

Associations between the morphological parameters of proximal tibiofibular joint (PTFJ) and changes in tibiofemoral joint structures in patients with knee osteoarthritis.

Arthritis Res Ther 2022 01 27;24(1):34. Epub 2022 Jan 27.

Clinical Research Centre, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China.

Background: To describe the longitudinal associations between the morphological parameters of proximal tibiofibular joint (PTFJ) and joint structural changes in tibiofemoral compartments in patients with knee osteoarthritis (OA).

Methods: The participants were selected from the Vitamin D Effects on Osteoarthritis (VIDEO) study. PTFJ morphological parameters were measured on coronal and sagittal MRI. The contacting area (S) of PTFJ and its projection areas onto the horizontal (load-bearing area, Sτ), sagittal (lateral stress-bolstering area, Sφ), and coronal plane (posterior stress-bolstering area, Sυ) were assessed. Knee structural abnormalities, including cartilage defects, bone marrow lesions (BMLs), and cartilage volume, were evaluated at baseline and after 2 years. Log binominal regression models and linear regression models were used to assess the associations between PTFJ morphological parameters and osteoarthritic structural changes.

Results: In the longitudinal analyses, the S (RR: 1.45) and Sτ (RR: 1.55) of PTFJ were significantly and positively associated with an increase in medial tibial (MT) cartilage defects. The Sτ (β: - 0.07), Sυ (β: - 0.07), and S (β: - 0.06) of PTFJ were significantly and negatively associated with changes in MT cartilage volume. The Sτ (RR: 1.55) of PTFJ was positively associated with an increase in MT BMLs, and Sφ (RR: 0.35) was negatively associated with an increase in medial femoral BMLs.

Conclusions: This longitudinal study suggests that higher load-bearing area of PTFJ could be a risk factor for structural changes in medial tibiofemoral (MTF) compartment in knee OA.

Trial Registration: Clinicaltrials.gov Identifier: NCT01176344 Anzctr.org.au Identifier: ACTRN12610000495022 Date of registration: 7 May 2010.
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http://dx.doi.org/10.1186/s13075-022-02719-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8793191PMC
January 2022

Endogenous Aβ induces osteoporosis through an mTOR-dependent inhibition of autophagy in bone marrow mesenchymal stem cells (BMSCs).

Ann Transl Med 2021 Dec;9(24):1794

Department of Foot and Ankle Surgery, Center for Orthopedic Surgery, the Third Affiliated Hospital of Southern Medical University, Guangzhou, China.

Background: It has previously been suggested that Alzheimer's disease (AD) and osteoporosis (OP) were related. However, the connection between these 2 disorders is poorly understood. This study aimed to investigate the relationship between amyloid β peptide (Aβ) and the osteoporotic deficit observed in AD patients.

Methods: We used the APP/PS1ΔE9 transgenic mouse model of AD for study and extracted bone marrow mesenchymal stem cells (BMSCs) for in vitro studies. For experiments, mice femurs were put through a μ-computer tomography (μ-CT) scanning and after which, sliced for hematoxylin/eosin (HE), Masson and Goldner staining for detection of bone changes. For experiments, BMSCs were placed in an osteogenic inducing medium with or without rapamycin. After induction, alkaline phosphatase (ALP) staining, alizarin red staining, quantitative real-time PCR (qPCR) and western-blot were used to identify osteogenic differentiation, calcium deposition and protein expression differences respectively.

Results: We observed that pathological changes characteristic of AD and OP occurred in vivo in APP/PS1ΔE9 mice. In BMSCs producing endogenous Aβ, mammalian target of rapamycin (mTOR) activation and subsequent inhibition of autophagy suppressed bone formation. Further, the addition of the mTOR inhibitor rapamycin into the inducing medium reversed the inhibition of osteogenesis.

Conclusions: Our results suggested that endogenous Aβ might have induced osteoporosis through an mTOR-dependent inhibition of autophagy in BMSCs, which may explain the OP changes observed in AD patients.
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http://dx.doi.org/10.21037/atm-21-6427DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8756236PMC
December 2021

(+)- and (-)-trichodermatrione A: a pair of enantiomers with a cyclobutane-containing skeleton from the endophytic fungus Trichoderma sp. EFT2.

Phytochemistry 2022 Apr 11;196:113087. Epub 2022 Jan 11.

State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, PR China; Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, PR China. Electronic address:

(±)-trichodermatrione A, a pair of cyclobutane-containing enantiomers with an undescribed tricyclic 6/4/6 skeleton, was isolated from Trichoderma sp. EFT2, an endophytic fungus from Euonymus fortunei (Turcz.) Hand.-Mazz (Celastraceae). The racemates were separated by chiral HPLC with the structures elucidated by a combination of MS, NMR, ECD calculation and X-ray crystallography analyses. (±)- trichodermatrione A and enantiomers were found to be antibacterial against phytopathogenic bacteria Xanthomonas oryzae pv. oryzae (Xoo) and X. oryzae pv. oryzicola (Xoc).
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http://dx.doi.org/10.1016/j.phytochem.2022.113087DOI Listing
April 2022

A Novel Calibration Method for Gyro-Accelerometer Asynchronous Time in Foot-Mounted Pedestrian Navigation System.

Sensors (Basel) 2021 Dec 29;22(1). Epub 2021 Dec 29.

School of Instrumentation and Optoelectronic Engineering, Beihang University, Beijing 100191, China.

Pedestrian Navigation System (PNS) is one of the research focuses of indoor positioning in GNSS-denied environments based on the MEMS Inertial Measurement Unit (MIMU). However, in the foot-mounted pedestrian navigation system with MIMU or mobile phone as the main carrier, it is difficult to make the sampling time of gyros and accelerometers completely synchronous. The gyro-accelerometer asynchronous time affects the positioning of PNS. To solve this problem, a new error model of gyro-accelerometer asynchronous time is built. The effect of gyro-accelerometer asynchronous time on pedestrian navigation is analyzed. A filtering model is designed to calibrate the gyro-accelerometer asynchronous time, and a zero-velocity detection method based on the rate of attitude change is proposed. The indoor experiment shows that the gyro-accelerometer asynchronous time is estimated effectively, and the positioning accuracy of PNS is improved by the proposed method after compensating for the errors caused by gyro-accelerometer asynchronous time.
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http://dx.doi.org/10.3390/s22010209DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8749666PMC
December 2021

Effect of the Length of Oat Hay on Growth Performance, Health Status, Behavior Parameters and Rumen Fermentation of Holstein Female Calves.

Metabolites 2021 Dec 20;11(12). Epub 2021 Dec 20.

Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control, State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.

The aim of this study was to evaluate the effect of the length of oat hay on the performance, health, behavior, and rumen fermentation of dairy calves. For this purpose, two hundred and ten healthy two-day-old Holstein dairy calves were randomly allocated into three groups: basic diet (calf starter) without hay (CON), or a basic diet with oat hay at either long (OL: 10-12 cm) or short (OS: 3-5 cm) length cut. The basic diet was fed from day 4, while the hay was offered from day 14. All calves were weaned at day 56 and remained in their individual hutches till the end of the trial (day 70). Calf starter intake and fecal scores were recorded daily. Bodyweight, body size, and rumen fluid samples were collected biweekly before weaning and weekly after weaning. Overall, providing oat hay (OS and OL) in the diet increased the body weight, starter intake, and average daily gain compared to the CON group. Similarly, feeding oat hay improved rumen fermentation. More specifically, hay enhanced the rumen pH and changed the rumen fermentation type. Hay fed calves spent more time on rumination but less time performing abnormal behaviors compared to control. As it can be concluded, feeding oat hay to calves enhances the growth performance, rumen fermentation, and normal calf behaviors, implying improved animal welfare irrespective of the hay length.
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http://dx.doi.org/10.3390/metabo11120890DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8703666PMC
December 2021

Biological Effect of Differently Sized Tetrahedral Framework Nucleic Acids: Endocytosis, Proliferation, Migration, and Biodistribution.

ACS Appl Mater Interfaces 2021 Dec 22;13(48):57067-57074. Epub 2021 Nov 22.

State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.

With the advent of nanotechnology, DNA nanostructures have been widely applied in various fields, particularly biology and biomedicine. Tetrahedral framework nucleic acids (TFNAs), a novel type of DNA nanomaterial, have attracted considerable attention due to their simple synthesis, high accessibility, structural stability, and versatility. However, to date, the interaction of differently sized TFNAs with living systems and their ability to be endocytosed and biodistributed in mouse is still not fully understood. To screen for the optimal TFNA size and structures, TFNA endocytosis, proliferation, and migration were tested in adipose stem cells (ASCs). We found that the internalization of differently sized TFNAs in ASCs was remarkably different. Although all TFNAs could enter ASCs, T21 had the best membrane-penetrating ability. After exposure of ASCs to TFNAs of different sizes, the proliferation and migration of cells were enhanced, especially with T21. Importantly, T21 could access the brain and accumulate over time. This study improves our understanding of the influence of TFNA size on the biological behavior of ASCs, which will help in choosing optimal TFNA size for biomedical applications.
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http://dx.doi.org/10.1021/acsami.1c20657DOI Listing
December 2021

Modelling the Shear Banding in Gradient Nano-Grained Metals.

Nanomaterials (Basel) 2021 Sep 22;11(10). Epub 2021 Sep 22.

College of Mechanical and Electrical Engineering, Central South University, Changsha 410083, China.

Extensive experiments have shown that gradient nano-grained metals have outstanding synergy of strength and ductility. However, the deformation mechanisms of gradient metals are still not fully understood due to their complicated gradient microstructure. One of the difficulties is the accurate description of the deformation of the nanocrystalline surface layer of the gradient metals. Recent experiments with a closer inspection into the surface morphology of the gradient metals reported that shear bands (strain localization) occur at the surface of the materials even under a very small, applied strain, which is in contrast to previously suggested uniform deformation. Here, a dislocation density-based computational model is developed to investigate the shear band evolution in gradient Cu to overcome the above difficulty and to clarify the above debate. The Voronoi polygon is used to establish the irregular grain structure, which has a gradual increase in grain size from the material surface to the interior. It was found that the shear band occurs at a small applied strain in the surface region of the gradient structure, and multiple shear bands are gradually formed with increasing applied load. The early appearance of shear banding and the formation of abundant shear bands resulted from the constraint of the coarse-grained interior. The number of shear bands and the uniform elongation of the gradient material were positively related, both of which increased with decreasing grain size distribution index and gradient layer thickness or increasing surface grain size. The findings are in good agreement with recent experimental observations in terms of stress-strain responses and shear band evolution. We conclude that the enhanced ductility of gradient metals originated from the gradient deformation-induced stable shear band evolution during tension.
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http://dx.doi.org/10.3390/nano11102468DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8541060PMC
September 2021

The Antioxidant Properties of Selenium and Vitamin E; Their Role in Periparturient Dairy Cattle Health Regulation.

Antioxidants (Basel) 2021 Sep 29;10(10). Epub 2021 Sep 29.

Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control, State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.

Dairy cattle experience health risks during the periparturient period. The continuous overproduction of reactive oxygen species (ROS) during the transition from late gestation to peak lactation leads to the development of oxidative stress. Oxidative stress is usually considered the main contributor to several diseases such as retained placenta, fatty liver, ketosis, mastitis and metritis in periparturient dairy cattle. The oxidative stress is generally balanced by the naturally available antioxidant system in the body of dairy cattle. However, in some special conditions, such as the peripariparturient period, the natural antioxidant system of a body is not able to balance the ROS production. To cope with this situation, the antioxidants are supplied to the dairy cattle from external sources. Natural antioxidants such as selenium and vitamin E have been found to restore normal health by minimizing the harmful effects of excessive ROS production. The deficiencies of Se and vitamin E have been reported to be associated with various diseases in periparturient dairy cattle. Thus in the current review, we highlight the new insights into the Se and vitamin E supplementation as antioxidant agents in the health regulation of periparturient dairy cattle.
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http://dx.doi.org/10.3390/antiox10101555DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8532922PMC
September 2021

Ni/Fe layered double hydroxide nanosheet/G-quadruplex as a new complex DNAzyme with highly enhanced peroxidase-mimic activity.

Analyst 2021 Oct 25;146(21):6470-6473. Epub 2021 Oct 25.

School of Environmental and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang 212003, Jiangsu Province, PR China.

A novel and low-cost DNAzyme, Ni/Fe layered double hydroxide (LDH) nanosheet/G-quadruplex (without hemin) with enhanced peroxidase-mimic activity was designed. The catalytic mechanism was investigated. The detection of Cu(II) in actual serum samples could be realized sensitively this efficient DNAzyme-based method.
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http://dx.doi.org/10.1039/d1an01405fDOI Listing
October 2021

Osteoarthritic infrapatellar fat pad aggravates cartilage degradation via activation of p38MAPK and ERK1/2 pathways.

Inflamm Res 2021 Dec 25;70(10-12):1129-1139. Epub 2021 Sep 25.

Clinical Research Centre, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China.

Objective: This study aimed to investigate the biochemical effects of osteoarthritic infrapatellar fat pad (IPFP) on cartilage and the underlying mechanisms.

Methods: Human IPFP and articular cartilage were collected from end-stage osteoarthritis (OA) patients during total knee arthroplasty. IPFP-derived fat-conditioned medium (FCM) was used to stimulate human primary chondrocytes and cartilage explants. Functional effect of osteoarthritic IPFP was explored in human primary chondrocytes and articular cartilage in vitro and ex vivo. Activation of relative pathways and its effects on chondrocytes were assessed through immunoblotting and inhibition experiments, respectively. Neutralization test was performed to identify the main factors and their associated pathways responsible for the effects of IPFP.

Results: Osteoarthritic IPFP-derived FCM significantly induced extracellular matrix (ECM) degradation in both human primary chondrocytes and cartilage explants. Several pathways, such as NF-κB, mTORC1, p38MAPK, JNK, and ERK1/2 signaling, were significantly activated in human chondrocytes with osteoarthritic IPFP-derived FCM stimulation. Interestingly, inhibition of p38MAPK and ERK1/2 signaling pathway could alleviate the detrimental effects of FCM on chondrocytes, while inhibition of other signaling pathways had no similar results. In addition, IL-1β and TNF-α instead of IL-6 in osteoarthritic IPFP-derived FCM played key roles in cartilage degradation via activating p38MAPK rather than ERK1/2 signaling pathway.

Conclusion: Osteoarthritic IPFP induces the degradation and inflammation of cartilage via activation of p38MAPK and ERK1/2 pathways, in which IL-1β and TNF-α act as the key factors. Our study suggests that modulating the effects of IPFP on cartilage may be a promising strategy for knee OA intervention.
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http://dx.doi.org/10.1007/s00011-021-01503-9DOI Listing
December 2021

Tetrahedral Framework Nucleic Acids Ameliorate Insulin Resistance in Type 2 Diabetes Mellitus the PI3K/Akt Pathway.

ACS Appl Mater Interfaces 2021 Sep 19;13(34):40354-40364. Epub 2021 Aug 19.

State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, P. R. China.

Insulin resistance (IR) is one of the essential conditions in the development of type 2 diabetes mellitus (T2DM). IR occurs in hepatic cells when the insulin receptor substrate-1 (IRS-1)/phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway is downregulated; thus, activating this pathway can significantly improve insulin sensitivity and ameliorate T2DM. Tetrahedral framework nucleic acids (tFNAs), a DNA nanomaterial, are synthesized from four single-stranded DNA molecules. tFNAs possess excellent biocompatibility and good water solubility and stability. tFNAs can promote cell proliferation, cell autophagy, wound healing, and nerve regeneration by activating the PI3K/Akt pathway. Herein, we explore the effects and underlying mechanisms of tFNAs on IR. The results displayed that tFNAs could increase glucose uptake and ameliorate IR by activating the IRS-1/PI3K/Akt pathway in glucosamine (GlcN)-stimulated HepG2 cells. By employing a PI3K inhibitor, we confirmed that tFNAs reduce IR through the PI3K/Akt pathway. Moreover, tFNAs can promote hepatic cell proliferation and inhibit GlcN-induced cell apoptosis. In a T2DM mouse model, tFNAs reduce blood glucose levels and ameliorate hepatic IR the PI3K/Akt pathway. Taken together, tFNAs can improve hepatic IR and alleviate T2DM through the PI3K/Akt pathway, making contribution to the potential application of tFNAs in T2DM.
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http://dx.doi.org/10.1021/acsami.1c11468DOI Listing
September 2021

The silver linings of mercury: Reconsideration of its impacts on living organisms from a multi-timescale perspective.

Environ Int 2021 10 3;155:106670. Epub 2021 Jun 3.

State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, China; Environmental and Life Sciences Program (EnLS), Trent University, Peterborough, Ontario, Canada. Electronic address:

Research on mercury (Hg), a naturally occurring element in Earth's lithosphere, has been extremely hot in the past few decades due to the outbreak of a series of disastrous poisoning incidents. However, such studies might provide us a biased view towards Hg if no thorough review about its long-term effects on living organisms from a multi-timescale perspective was performed. Hg might have played a mysterious role in critical intervals (e.g., mass extinction and oceanic anoxia events) in several geologic periods due to the elevated Hg levels induced by volcanism whereas it has long been used for various purposes in human history. Therefore, it is necessary to go through previous studies and historical records of different timescales (10 to 10 yr). In this work, we conducted a thorough review of Hg knowledge at three different timescales, i.e., geologic periods (10 yr), human history (10 yr), and contemporary history (10 yr), summarizing recent advances and indicated potential research gaps. By doing so, we demonstrated that it is possible to achieve safe and sustainable Hg applications despite the current Hg crisis. However, such silver linings depend on a better understanding of ecosystem dynamics. Besides, considering the possible dire consequences of erupted Hg levels as suggested in geological periods, swift actions to mitigate the impacts of anthropogenic activities on the Hg cycle will be another key point. Overall, this review presented a unique perspective of Hg combining different timescales, shedding light on the importance of a better understanding of the global ecosystem as a whole and maintaining the sustainability of planet Earth in the future.
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http://dx.doi.org/10.1016/j.envint.2021.106670DOI Listing
October 2021

Determination of Vanadium Isotope Compositions in Carbonates Using an Fe Coprecipitation Method and MC-ICP-MS.

Anal Chem 2021 05 7;93(19):7172-7179. Epub 2021 May 7.

CAS Key Laboratory of Mantel Materials and Environments, School of Earth and Space Science, University of Science and Technology of China, 96 Jinzhai Road, Hefei 230026, Anhui, People's Republic of China.

Vanadium isotope compositions (δ(V)) in marine carbonates are a potential proxy to trace global redox states of ancient oceans. Although high-precision δ(V) analyses are available for many geological materials, carbonate-hosted δ(V) data have not been reported yet due to extremely high matrix elements and low V contents (generally below 10 μg g). In this study, we developed an Fe coprecipitation method combined with an Fe column to preconcentrate V from the major matrix elements and subsequent four-step chromatographic procedures to further purify V in carbonates. The δ(V) values were measured using a sample-standard bracketing method by MC-ICP-MS. The robustness of this method was assessed by analyzing element-doped and matrix-spiked synthetic carbonate solutions containing an in-house δ(V) standard, USTC-V. The mean δ(V) value of the synthetic carbonate solutions (0.06 ± 0.08‰; 2SD, = 33) is in good agreement with the recommended value of the USTC-V relative to the Oxford AA solution (0.07 ± 0.08‰; 2SD, = 347). In addition, the consistency in the δ(V) value of the igneous carbonatite standard, COQ-1, which was processed in parallel with the whole purification (-0.48 ± 0.04‰; 2SD, = 3) and a four-step chromatographic procedure (-0.43 ± 0.08‰; 2SD, = 3), further validates the robustness of our method. For the first time, we obtained δ(V) values of four carbonate reference materials: JDo-1, -0.56 ± 0.09‰ (2SD, = 27); JLs-1, -0.61 ± 0.14‰ (2SD, = 33); GBW07217a, -0.79 ± 0.09‰ (2SD, = 6); GBW07214a, -0.51 ± 0.13‰ (2SD, = 48). The long-term external precision of carbonate-hosted δ(V) analyses is better than ±0.14‰ (2SD). Our method can be applied to measure carbonate-hosted δ(V) to trace the evolution in global marine redox states throughout the Earth's history.
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http://dx.doi.org/10.1021/acs.analchem.0c04800DOI Listing
May 2021

Frontiers of Membrane Desalination Processes for Brackish Water Treatment: A Review.

Membranes (Basel) 2021 Mar 29;11(4). Epub 2021 Mar 29.

Department of Chemical Engineering, Texas Tech University, Lubbock, TX 79409, USA.

Climate change, population growth, and increased industrial activities are exacerbating freshwater scarcity and leading to increased interest in desalination of saline water. Brackish water is an attractive alternative to freshwater due to its low salinity and widespread availability in many water-scarce areas. However, partial or total desalination of brackish water is essential to reach the water quality requirements for a variety of applications. Selection of appropriate technology requires knowledge and understanding of the operational principles, capabilities, and limitations of the available desalination processes. Proper combination of feedwater technology improves the energy efficiency of desalination. In this article, we focus on pressure-driven and electro-driven membrane desalination processes. We review the principles, as well as challenges and recent improvements for reverse osmosis (RO), nanofiltration (NF), electrodialysis (ED), and membrane capacitive deionization (MCDI). RO is the dominant membrane process for large-scale desalination of brackish water with higher salinity, while ED and MCDI are energy-efficient for lower salinity ranges. Selective removal of multivalent components makes NF an excellent option for water softening. Brackish water desalination with membrane processes faces a series of challenges. Membrane fouling and scaling are the common issues associated with these processes, resulting in a reduction in their water recovery and energy efficiency. To overcome such adverse effects, many efforts have been dedicated toward development of pre-treatment steps, surface modification of membranes, use of anti-scalant, and modification of operational conditions. However, the effectiveness of these approaches depends on the fouling propensity of the feed water. In addition to the fouling and scaling, each process may face other challenges depending on their state of development and maturity. This review provides recent advances in the material, architecture, and operation of these processes that can assist in the selection and design of technologies for particular applications. The active research directions to improve the performance of these processes are also identified. The review shows that technologies that are tunable and particularly efficient for partial desalination such as ED and MCDI are increasingly competitive with traditional RO processes. Development of cost-effective ion exchange membranes with high chemical and mechanical stability can further improve the economy of desalination with electro-membrane processes and advance their future applications.
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http://dx.doi.org/10.3390/membranes11040246DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066301PMC
March 2021

Photocatalytic mechanism and performance of a novel wool flake-BiFeOnanosheet-TiOcore-shell-structured composite photocatalyst.

Nanotechnology 2021 Apr 12;32(27). Epub 2021 Apr 12.

Performance Textiles and Clothing Research Group, School of Design, University of Leeds, Leeds, LS2 9JT, United Kingdom.

In this study, BiFeO(BFO) nanosheets ground from BFO particles were first incorporated with wool flakes to construct sandwich-like wool-BFO composites using the vibration-assisted ball milling technique in freezing conditions. The wool-BFO composites were then loaded with a thick layer of TiOnanoparticles to prepare the core-shell-structured wool-BFO-TiOcomposites using a hydrothermal synthesis process. The microstructure of the core-shell wool-BFO-TiOcomposites and its photocatalytic applications were systematically examined using a series of characterization methods. Trapping experiments and electron spin resonance spectra were also employed to judge the active radical species like superoxide radicals (·O), singlet oxygen (O), holes (h), and hydroxyl radicals (·OH) using benzoquinone, furfuryl alcohol, ethylenediamine tetraacetic acid, and tert-butanol as the scavengers, respectively. The photodegradation performance of the wool-BFO-TiOcomposites was measured using more resistant methyl orange (MO) dye as the pollutant model. In comparison with the wool-TiOor wool-BFO composites, the superior photocatalytic properties of the wool-BFO-TiOcomposites under visible light irradiation were attributed to the presence of mesopores and macropores, the large specific surface area and intimate interface between wool-BFO composites and TiOnanoparticles, the coexistence of Fe, Fe, Bi, Bi, Ti, and Tispecies, and the strong visible light harvesting, thus leading to the fast separation of photogenerated electron-hole pairs. The wool-BFO-TiOcomposites could be used for the repeated photodegradation of organic pollutants and be recycled easily using a magnet. The active radical species of the wool-BFO-TiOcomposites were ·OandOrather than ·OH and h, which were involved in the photodegradation of MO dye under visible light irradiation.
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http://dx.doi.org/10.1088/1361-6528/abf072DOI Listing
April 2021

Different feed presentations affect subsequent feed sorting and rumen pH for a short period in weaned calves.

J Dairy Sci 2021 Apr 13;104(4):4146-4156. Epub 2021 Feb 13.

State Key Laboratory of Animal Nutrition, Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control, College of Animal Science and Technology, China Agricultural University, Beijing 100193, P. R. China. Electronic address:

The objective of this study was to investigate the short- and long-term effects of different feed presentations on feed sorting and rumen pH in weaned calves. Thirty-six weaned female calves at the age of 12 wk (78 d) were raised in pairs (18 pens; n = 6/treatment) and randomly exposed to 1 of 3 feed presentation treatments: (1) concentrate ration (CON, only exposed to concentrate); (2) separate ration (CH, exposed to concentrate and hay as separate components); and (3) mixed ration (Mix, exposed to a mixed diet containing 75% concentrate and 25% hay). After 4 wk (from d 78 to 105) on different feed presentations, all weaned calves were introduced to a novel total mixed ration (TMR) for another 12 wk (from d 106 to 189). Fresh feed and orts were sampled daily before (wk 12 to 15) and after (wk 16, 17, and 28) transitioning to a TMR diet for analysis of feed sorting. Rumen fluid was sampled in wk 12, 13, 15, 16, 17, and 28 to determine rumen pH. The performance of weaned calves was affected by the different feed presentations during the pre-changing period, such that calves fed CON had a lower dry matter intake (DMI) and average daily gain than calves fed CH and Mix diets. When calves were introduced to the Mix diet, they immediately developed a higher degree of sorting behavior against the long particle fractions. Upon transition to TMR, we did not observe any differences in the performance of calves. However, the sorting behavior established in Mix calves persisted and was similar to calves previously fed the CON diet, whereas the extent of feed sorting in calves initially fed CH was less compared with that in the other 2 treatments in wk 16 and 17. Before changing the diet was transitioned to a TMR, calves fed CON had a lower rumen pH than calves fed CH and Mix. Although rumen pH in all treatments increased to the same level after the diet changed, we observed a tendency toward lower rumen pH in calves fed Mix compared with calves fed CH at wk 17, which might have resulted from the higher degree of feed sorting in these calves. However, by the end of the experiment (wk 28), feed sorting and rumen pH were similar across all treatments. These results indicated a short-term effect of previous feed presentations on subsequent feed sorting and rumen pH, but in the long term disappeared.
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http://dx.doi.org/10.3168/jds.2020-19170DOI Listing
April 2021

Sphagnum Inspired g-C N Nano/Microspheres with Smaller Bandgap in Heterojunction Membranes for Sunlight-Driven Water Purification.

Small 2021 03 15;17(12):e2007122. Epub 2021 Feb 15.

Polymer Materials & Engineering Department, School of Materials Science & Engineering, Chang'an University, Xian, 710064, China.

Membrane separation is recognized as one of the most effective strategies to treat the complicated wastewater system for economic development. However, serious membrane fouling has restricted its further application. Inspired by sphagnum, a 0D/2D heterojunction composite membrane is engineered by depositing graphitic carbon nitride nano/microspheres (CNMS) with plentiful wrinkles onto the polyacrylic acid functionalized carbon nanotubes (CNTs-PAA) membrane through hydrogen bond force. Through coupling unique structure and chemistry properties, the CNTs-PAA/CNMS heterojunction membrane presents superhydrophilicity and underwater superoleophobicity. Furthermore, thanks to the J-type aggregates during the solvothermal process, it is provided with a smaller bandgap (1.77 eV) than the traditional graphitic carbon nitride (g-C N ) sheets-based membranes (2.4-2.8 eV). This feature endows the CNTs-PAA/CNMS membrane with superior visible-light-driven self-cleaning ability, which can maintain its excellent emulsion separation (with a maximum flux of 5557 ± 331 L m h bar and an efficiency of 98.5 ± 0.6%), photocatalytic degradation (with an efficiency of 99.7 ± 0.2%), and antibacterial (with an efficiency of ≈100%) ability even after cyclic experimental processes. The excellent self-cleaning performance of this all-in-one membrane represents its potential value for water purification.
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http://dx.doi.org/10.1002/smll.202007122DOI Listing
March 2021

Comparison of the visual performance between Oculentis MF30 and Tecnis ZMB00 multifocal intraocular lenses.

Ann Transl Med 2021 Jan;9(2):144

Cataract Department, Eye Hospital of Wenzhou Medical University, Wenzhou, China.

Background: To compare the visual performance of MF30 asymmetric refractive multifocal intraocular lenses (MIOLs) with ZMB00 all optic zone diffractive MIOLs.

Methods: This is a prospective study. Patients that underwent phacoemulsification were divided into two groups according to the type of MIOLs used: 35 patients were implanted with asymmetric refractive MIOLs and 35 patients with all optic zone diffractive MIOLs. Visual acuity (VA), refraction, defocus curves, objective optical quality, and a questionnaire evaluating quality of life were measured at 3 months postoperatively.

Results: There were no significant differences between the two groups in uncorrected distance visual acuity (UDVA), uncorrected near visual acuity (UNVA), best-corrected distance visual acuity (BCDVA), or distance-corrected near visual acuity (DCNVA). However, the uncorrected intermediate VA was 0.24±0.10 in the refractive group and 0.31±0.13 in the diffractive group (P<0.05), and the distance-corrected intermediate VA was 0.22±0.09 in the refractive group and 0.31±0.14 in the diffractive group (P<0.05). Defocus curves showed two peaks of maximum vision in both groups. However, the curve between the two peaks in the refractive group was smoother than that of the diffractive group. The modulated transfer function cut-off frequency was 22.74±12.29 c/d in the refractive group and 30.50±10.04 c/d in the diffractive group (P<0.05). The Optical Quality Analysis System (OQAS) values 100% (OV100%) was 0.75±0.41 in the refractive group and 1.02±0.34 in the diffractive group (P<0.05), while the OV20% was 0.52±0.34 in the refractive group and 0.71±0.25 in the diffractive group (P<0.05). There was no significant difference between the two groups in overall satisfaction, spectacle independence ratio, or visual interference phenomenon.

Conclusions: Both MIOLs achieve good VA at distance and near vision. Oculentis MF30 showed better intermediate VA, and Tecnis ZMB00 appears to have better objective visual quality.

Trial Registration: NCT02234635 (http://www.clinicaltrials.gov).
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http://dx.doi.org/10.21037/atm-20-7777DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7867926PMC
January 2021
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