Publications by authors named "TianJian Lu"

26 Publications

  • Page 1 of 1

Knockdown of miR-15b partially reverses the cisplatin resistance of NSCLC through the GSK-3β/MCL-1 pathway.

Adv Clin Exp Med 2021 Jul 20. Epub 2021 Jul 20.

Department of Anesthesia, Nanhua University, Hengyang, China.

Background: Induction of acquired drug resistance occurs frequently with cisplatin-based therapy for non-small cell lung cancer (NSCLC). As recent studies have demonstrated that deregulation of microRNAs (miRNAs) is associated with drug resistance in cancers, correcting the deregulation of miRNAs represents a promising strategy to reverse acquired resistance in NSCLC.

Objectives: This study investigated the functional role of miR-15b in cisplatin resistance in NSCLC.

Material And Methods: Cisplatin-resistant PC9 and A549 NSCLC cell lines (PC9-R and A549-R) were established through long-term exposure to cisplatin. Differences in miR-15b expression between cisplatin-resistant NSCLC cell lines and their parental cell lines were identified through quantitative real-time polymerase chain reaction (qRT-PCR). The effect of anti-miR-15b on the sensitivity of PC9-R and A549-R to cisplatin-induced cytotoxicity was evaluated using Cell Counting Kit-8 (CCK-8) assays. Regulation of GSK-3β by miR-15b was confirmed with luciferase reporter assays. Cell apoptosis and mitochondrial membrane potential (MMP) were measured using flow cytometry analysis.

Results: In PC9-R and A549-R cells, miR-15b was significantly overexpressed. However, knockdown of miR-15b clearly reduced cisplatin resistance in PC9-R and A549-R cells. Researching the mechanism, we proved that GSK-3β was the target of miR-15b. Knockdown of miR-15b significantly increased the expression GSK-3β and thus promoted the degradation of MCL-1, which is a key anti-apoptosis protein. As a result, anti-miR-15b expanded the cisplatin-induced apoptosis in cisplatin-resistant NSCLC cells.

Conclusions: Knockdown of miR-15b partially reversed cisplatin resistance in NSCLC cells through the GSK-3β/MCL-1 pathway.
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http://dx.doi.org/10.17219/acem/135701DOI Listing
July 2021

Application of burning treatment with high-frequency electric knife of clearing tumor in non-small cell lung cancer patients with adjacent organ invasion: A single-central experience.

Asian J Surg 2021 Jun 22. Epub 2021 Jun 22.

Department of Thoracic Surgery, Jilin Cancer Hospital, No.1018, Huguang Road, Changchun, 130000, Jilin, China; Changchun Tumor Hospital, No.3355, Guigu St, Changchun, 130000, Jilin, China. Electronic address:

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http://dx.doi.org/10.1016/j.asjsur.2021.05.032DOI Listing
June 2021

A new basic thoracoscopic surgical skill training and assessment system using automatic scoring techniques.

Surg Endosc 2021 Jun 24. Epub 2021 Jun 24.

Department of Thoracic Surgery, West China Hospital, Sichuan University, No. 37, Guoxue Alley, Chengdu, Sichuan, 610041, China.

Purpose: We report a new thoracoscopic surgical skill training and assessment system with automatic scoring techniques, the Huaxi Intelligent Thoracoscopic Skill Training and Assessment (HITSTA) system. We also evaluated the discriminative ability of this system compared to our conventional scoring method at our institution.

Methods: We retrospectively collected training data of thoracic board-certified thoracic surgeons at West China Hospital, Sichuan University from January 1, 2018 to January 1, 2019. Surgeons were assessed by HITSTA system and human examiners simultaneously. Total scores were summed from 3 tasks (grasping with delivery, pattern cutting, and suture with knot). Bland-Altman analysis was used to test agreement of scores made by HITSTA system (automatic scoring) and human examiners (manual scoring). Differentiation ability was also compared between the two scoring methods.

Results: Thirty-nine surgeons were recruited. Scores made by HITSTA system and human examiners were not consistent. For suture with knot, automatic scoring method could detect the score differences between different training status (trained: 26.92 ± 12.04, untrained: 19.85 ± 11.12; p = 0.026) and training duration (< 10 h: 20.67 ± 15.23,  ≥ 10 h: 31.92 ± 5.56; p = 0.003). For total scores, automatic scoring approach could discriminate between different training status (trained: 71.90 ± 12.63; untrained: 61.41 ± 13.87; p = 0.016) and training duration (< 10 h: 65.23 ± 15.31;  ≥ 10 h 77.23 ± 6.94; p = 0.046).

Conclusion: HITSTA system could discriminate the different levels of thoracoscopic surgical skills better than the traditional manual scoring method. Larger prospective studies are warranted to validate the differentiation ability of HITSTA system.
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http://dx.doi.org/10.1007/s00464-021-08606-8DOI Listing
June 2021

Thermal Volume Reduction Surgery for Surgical Treatment of Pulmonary Bullae: A Single-Center Treatment Experience of 276 Cases Accompany With Primary Lung Cancer.

Front Surg 2021 4;8:672688. Epub 2021 May 4.

Department of Thoracic Surgery, Jilin Cancer Hospital, Changchun, China.

Lung volume reduction surgery (LVRS) has been regarded as an effective surgical procedure for severe emphysema (including pulmonary bullae). However, there still remain controversial that its applications limited that only patients with a specific clinical situation may benefit from LVRS, and so did other non-surgical treatments. The current study aims to introduce some initial experience of new technique for treating pulmonary bullae, including using thermal surgical instruments to reduce enlargement of lung tissue in a specific group that diagnosed with lung cancer accompany with pulmonary bullae. This retrospective study included 276 patients undergoing emphysema reducing surgery between 2010 and 2020. All procedure were performed by thermal volume reduction surgery of using thermal surgical instruments to reduce pulmonary bullae. The average time required for operating single pulmonary bullae was <10 min. Median operative time was 106 min (range 85 to 191 min). No intraoperative air leak, massive blood loss, or other severe complications occurred. The estimated blood loss for TVRS was about 40 ml (range 15 to 120 ml). Postoperative complications included atelectasis ( = 8), pulmonary infection ( = 17), bleeding ( = 5), delayed air leak ( = 7) among the cohort. The postoperative lung function at 1-year post surgery in TVRS group recovered faster with a better recovery that achieving an FEV1 of 1.95 ± 0.46 L, TLC of 6.36 ± 0.79 L, RV of 3.56 ± 0.81 L, PO of 60 ± 8 mmHg, PCO of 37 ± 6 mmHg, and 6 MWD (6-min walk distant) of 305 ± 22 m. The 1-year QOL score was elevated comparing with preoperative period. This single-center study reported a new thermal-based surgical approach to treat pulmonary bullae by reducing abnormally enlarged lung tissue in specific patients diagnosed with lung cancer accompany with pulmonary bullae.
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http://dx.doi.org/10.3389/fsurg.2021.672688DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129511PMC
May 2021

Eriodictyol inhibits high glucose-induced oxidative stress and inflammation in retinal ganglial cells.

J Cell Biochem 2019 04 14;120(4):5644-5651. Epub 2018 Oct 14.

Bioinspired Engineering and Biomechanics Center (BEBC), MOE Key Laboratory of Biomedical Information Engineering, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, China.

Diabetic retinopathy (DR) is one of the most common microvascular complications of diabetes mellitus and is considered as a leading cause of blindness. Oxidative stress and inflammation are significant drivers for the development of DR. Eriodictyol, a flavonoid compound, was proved to possess anti-inflammatory, antioxidative, and antidiabetic activities. However, the role of eriodictyol in DR has not been unveiled. In the current study, we explored the protective effects of eriodictyol on high glucose (HG)-induced rat retinal ganglial cells (RGCs). The results suggested that eriodictyol improved cell viability of HG-induced rat RGC-5 cells in a dose-dependent manner. Eriodictyol reduced the reactive oxygen species production and increased the activities of superoxide dismutase, glutathione peroxidase and catalase in rat RGC-5 cells in response to HG stimulation. The production of proinflammatory cytokines including tumor necrosis factor alpha and interleukin-8 was diminished after eriodictyol treatment. Eriodictyol also suppressed cell apoptosis induced HG in rat RGC-5 cells. Furthermore, eriodictyol enhanced the nuclear translocation of nuclear factor erythroid-2 (E2)-related factor 2 (Nrf2) and elevated the expression of antioxidant enzyme heme-oxygenase-1 (HO-1). These findings suggested that eriodictyol protects the RGC-5 cells from HG-induced oxidative stress, inflammation, and cell apoptosis through regulating the activation of Nrf2/HO-1 pathway.
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http://dx.doi.org/10.1002/jcb.27848DOI Listing
April 2019

Sound absorption by acoustic microlattice with optimized pore configuration.

J Acoust Soc Am 2018 Aug;144(2):EL138

State Key Laboratory for Strength and Vibration of Mechanical Structures, Xi'an Jiaotong University, Xi'an, Shanxi 710049, People's Republic of China

The great progress in material science and nano-micro fabrication enables the applications of metamaterials with well-defined and well-organized microstructures for noise reduction. However, what intrinsic morphology of the metamaterial would result in optimum sound absorbing efficiency remains uncertain. This work presents a microlattice metamaterial, comprising well-defined and organized material morphology in terms of pore size and porosity, for generating optimum sound dissipation. A compact governing equation is established and verified experimentally to show that the optimum sound absorption can only be reached when the pore size equals twice the thickness of a viscous boundary layer.
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http://dx.doi.org/10.1121/1.5051526DOI Listing
August 2018

Clinical characteristics and outcomes of lung cancer patients with combined pulmonary fibrosis and emphysema: a systematic review and meta-analysis of 13 studies.

J Thorac Dis 2017 Dec;9(12):5322-5334

Department of Thoracic Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.

Background: The characteristic and outcomes of lung cancer patients with combined pulmonary fibrosis and emphysema (CPFE) have long been assessed, but results were controversial. Therefore, we performed a meta-analysis to assess the clinical features and prognosis of lung cancer patients with CPFE.

Methods: The databases PubMed, Embase, and Web of Science (updated to October 1, 2017) were searched for eligible studies. Pooled odds ratios (ORs), weighted mean differences (WMD) or hazard ratios (HRs) with 95% confidence intervals (95% CIs) were used to evaluate the clinicopathological characteristics, the short-term outcome after operation and long-term survival of lung cancer patients with CPFE compared with lung cancer patients without CPFE (fibrosis, emphysema, and normal).

Results: Thirty original studies with 8,050 patients were included in this meta-analysis. The pooled results indicated that lung cancer patients with CPFE were associated with higher age (MD =3.39; 95% CI: 2.12-4.67, P<0.001), male (OR =8.46; 95% CI: 6.36-11.26, P<0.001), ex- or current smoker (OR =39.65; 95% CI: 15.64-100.5, P<0.001), longer smoking history (MD =15.56; 95% CI: 3.73-27.39, P=0.01), lower DLCO% (MD =-13.82; 95% CI: -21.4 to -6.24, P<0.001), squamous cell carcinoma histology (OR =3.55; 95% CI: 2.49-5.05, P<0.001), the lower lobes (OR =1.92; 95% CI: 1.52-2.43, P<0.001), advanced pathological stage (OR =1.55; 95% CI: 1.22-1.96, P<0.001). Lung cancer patients with CPFE had higher 30-day mortality (OR =4.72, 95% CI: 2.06-10.85, P<0.001), 90-day mortality (OR =5.33; 95% CI: 1.39-20.42, P=0.01), and incidence of postoperative complications (OR =5.25, 95% CI: 2.38-11.57, P<0.001). In addition, the lung cancer patients with CPFE had a poorer OS (HR =2.006, 95% CI: 1.347-2.986, P=0.001) than lung cancer patients without CPFE.

Conclusions: This meta-analysis demonstrated that lung cancer patients with CPFE have more aggressive clinical characteristic and a poor prognosis, suggesting that lung cancer patients with CPFE should be early detected, treated reasonably and be taken good care of.
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http://dx.doi.org/10.21037/jtd.2017.12.72DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5756974PMC
December 2017

Diffraction phase microscopy imaging and multi-physics modeling of the nanoscale thermal expansion of a suspended resistor.

Sci Rep 2017 07 4;7(1):4602. Epub 2017 Jul 4.

Photonic Systems Laboratory, Department of Electrical and Computer Engineering, Micro and Nanotechnology Lab, University of Illinois at Urbana-Champaign, Urbana, Illinois, 61801, USA.

We studied the nanoscale thermal expansion of a suspended resistor both theoretically and experimentally and obtained consistent results. In the theoretical analysis, we used a three-dimensional coupled electrical-thermal-mechanical simulation and obtained the temperature and displacement field of the suspended resistor under a direct current (DC) input voltage. In the experiment, we recorded a sequence of images of the axial thermal expansion of the central bridge region of the suspended resistor at a rate of 1.8 frames/s by using epi-illumination diffraction phase microscopy (epi-DPM). This method accurately measured nanometer level relative height changes of the resistor in a temporally and spatially resolved manner. Upon application of a 2 V step in voltage, the resistor exhibited a steady-state increase in resistance of 1.14 Ω and in relative height of 3.5 nm, which agreed reasonably well with the predicted values of 1.08 Ω and 4.4 nm, respectively.
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http://dx.doi.org/10.1038/s41598-017-04803-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5496882PMC
July 2017

Tensional acoustomechanical soft metamaterials.

Sci Rep 2016 06 6;6:27432. Epub 2016 Jun 6.

State Key Laboratory for Strength and Vibration of Mechanical Structures, Xi'an Jiaotong University, Xi'an 710049, P.R. China.

We create acoustomechanical soft metamaterials whose response to uniaxial tensile stressing can be easily tailored by programming acoustic wave inputs, resulting in force versus stretch curves that exhibit distinct monotonic, s-shape, plateau and non-monotonic snapping behaviors. We theoretically demonstrate this unique metamaterial by considering a thin soft material sheet impinged by two counter-propagating ultrasonic wave inputs across its thickness and stretched by an in-plane uniaxial tensile force. We establish a theoretical acoustomechanical model to describe the programmable mechanics of such soft metamaterial, and introduce the first- and second-order tangential stiffness of its force versus stretch curve to boundary different behaviors that appear during deformation. The proposed phase diagrams for the underlying nonlinear mechanics show promising prospects for designing tunable and switchable photonic/phononic crystals and microfluidic devices that harness snap-through instability.
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http://dx.doi.org/10.1038/srep27432DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4893735PMC
June 2016

Molecular analysis of interactions between a PAMAM dendrimer-paclitaxel conjugate and a biomembrane.

Phys Chem Chem Phys 2015 Nov 10;17(44):29507-17. Epub 2015 Aug 10.

Key Laboratory of Thermo-Fluid Science and Engineering of Ministry of Education, School of Energy and Power Engineering, Xi'an Jiaotong University, Xi'an 710049, P. R. China.

Understanding the underlying mechanism of nanomedicine-biomembrane interactions is important for the design and optimization of payload delivery systems. This study investigates the interactions between polyamidoamine (PAMAM) dendrimer-paclitaxel conjugates and biomembranes using coarse-grained molecular dynamics simulations. We found that acidic conditions (e.g., pH ∼ 5) and membrane asymmetry can improve the conjugate penetration. Paclitaxel (PTX) distributions on a G4 PAMAM dendrimer can affect interactions via the penetration mechanism, although they have no significant effect on interactions via the adsorption mechanism. The random distribution of PTX can enhance the ability of PTX molecules to pass through asymmetric membranes. Furthermore, the penetration process becomes more difficult with increasing paclitaxel loading ratios. These results provide molecular insights into the precise translocation mechanism of dendrimer-drug conjugates and thus provide suggestions for drug design and delivery.
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http://dx.doi.org/10.1039/c5cp02242hDOI Listing
November 2015

Microbubble embedded with upconversion nanoparticles as a bimodal contrast agent for fluorescence and ultrasound imaging.

Nanotechnology 2015 Aug;26(34):345601

Bimodal imaging offers additional imaging signal thus finds wide spread application in clinical diagnostic imaging. Fluorescence/ultrasound bimodal imaging contrast agent using fluorescent dyes or quantum dots for fluorescence signal has emerged as a promising method, which however requires visible light or UV irradiation resulting in photobleaching, photo blinking,auto-fluorescence and limited tissue penetration depth. To surmount these problems, we developed a novel bimodal contrast agent using layer-by-layer assembly of up conversion nanoparticles onto the surface of microbubbles. The resulting microbubbles with average size of 2 μm provide enhanced ultrasound echo for ultrasound imaging and upconversion emission upon near infrared irradiation for fluorescence imaging. The developed bimodal contrast agent holds great potential to be applied in ultrasound target technique for targeted diseases diagnostics and therapy.
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http://dx.doi.org/10.1088/0957-4484/26/34/345601DOI Listing
August 2015

Thermal Pain in Teeth: Electrophysiology Governed by Thermomechanics.

Appl Mech Rev 2014 May 18;66(3):0308011-3080114. Epub 2014 Apr 18.

Bioinspired Engineering and Biomechanics Center, Xi'an Jiaotong University , Xi'an 710049 , China e-mail: 

Thermal pain arising from the teeth is unlike that arising from anywhere else in the body. The source of this peculiarity is a long-standing mystery that has begun to unravel with recent experimental measurements and, somewhat surprisingly, new thermomechanical models. Pain from excessive heating and cooling is typically sensed throughout the body through the action of specific, heat sensitive ion channels that reside on sensory neurons known as nociceptors. These ion channels are found on tooth nociceptors, but only in teeth does the pain of heating differ starkly from the pain of cooling, with cold stimuli producing more rapid and sharper pain. Here, we review the range of hypotheses and models for these phenomena, and focus on what is emerging as the most promising hypothesis: pain transduced by fluid flowing through the hierarchical structure of teeth. We summarize experimental evidence, and critically review the range of heat transfer, solid mechanics, fluid dynamics, and electrophysiological models that have been combined to support this hypothesis. While the results reviewed here are specific to teeth, this class of coupled thermomechanical and neurophysiological models has potential for informing design of a broad range of thermal therapies and understanding of a range of biophysical phenomena.
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http://dx.doi.org/10.1115/1.4026912DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4240033PMC
May 2014

Microwave purification of large-area horizontally aligned arrays of single-walled carbon nanotubes.

Nat Commun 2014 Nov 12;5:5332. Epub 2014 Nov 12.

Department of Materials Science and Engineering, Frederick Seitz Materials Research Laboratory, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.

Recent progress in the field of single-walled carbon nanotubes (SWNTs) significantly enhances the potential for practical use of this remarkable class of material in advanced electronic and sensor devices. One of the most daunting challenges is in creating large-area, perfectly aligned arrays of purely semiconducting SWNTs (s-SWNTs). Here we introduce a simple, scalable, large-area scheme that achieves this goal through microwave irradiation of aligned SWNTs grown on quartz substrates. Microstrip dipole antennas of low work-function metals concentrate the microwaves and selectively couple them into only the metallic SWNTs (m-SWNTs). The result allows for complete removal of all m-SWNTs, as revealed through systematic experimental and computational studies of the process. As one demonstration of the effectiveness, implementing this method on large arrays consisting of ~20,000 SWNTs completely removes all of the m-SWNTs (~7,000) to yield a purity of s-SWNTs that corresponds, quantitatively, to at least to 99.9925% and likely significantly higher.
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http://dx.doi.org/10.1038/ncomms6332DOI Listing
November 2014

[Advances in the application of smart phones in modern medicine].

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 2014 Feb;31(1):222-7

Since smart phones have been developed, significant advances in the function of mobile phone due to the development of software, hardware and accessories have been reached. Till now, smart phones have been engaged in daily life with an increasing impact. As a new medical model, mobile phone medicine is emerging and has found wide spread applications in medicine, especially in diagnosing, monitoring and screening various diseases. In addition, mo bile phone medical application shows great potential trend to improve healthcare in resource-limited regions due to its advantageous features of portability and information communication capability. Nowadays, the scientific and technological issues related to mobile phone medicine have attracted worldwide attention. In this review, we summarize state-of-the-art advances of mobile phone medicine with focus on its diagnostics applications in order to expand the fields of their applications and promote healthcare informatization.
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February 2014

Molecular analysis of interactions between dendrimers and asymmetric membranes at different transport stages.

Soft Matter 2014 Jan;10(1):139-48

Key Laboratory of Thermo-Fluid Science and Engineering of Ministry of Education, School of Energy and Power Engineering, Xi'an Jiaotong University, Xi'an 710049, P.R. China.

Studying dendrimer-biomembrane interactions is important for understanding drug and gene delivery. In this study, coarse-grained molecular dynamics simulations were performed to investigate the behaviors of polyamidoamine (PAMAM) dendrimers (G4 and G5) as they interacted with asymmetric membranes from different sides of the bilayer, thus mimicking different dendrimer transport stages. The G4 dendrimer could insert into the membrane during an equilibrated state, and the G5 dendrimer could induce pore formation in the membrane when the dendrimers interacted with the outer side (outer interactions) of an asymmetric membrane [with 10% dipalmitoyl phosphatidylserine (DPPS) in the inner leaflet of the membrane]. During the interaction with the inner side of the asymmetric membrane (inner interactions), the G4 and G5 dendrimers only adsorbed onto the membrane. As the membrane asymmetry increased (e.g., increased DPPS percentage in the inner leaflet of the membrane), the G4 and G5 dendrimers penetrated deeper into the membrane during the outer interactions and the G4 and G5 dendrimers were adsorbed more tightly onto the membrane for the inner interactions. When the DPPS content reached 50%, the G4 dendrimer could completely penetrate through the membrane from the outer side to the inner side. Our study provides molecular understanding and reference information about different dendrimer transport stages during drug and gene delivery.
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http://dx.doi.org/10.1039/c3sm51990bDOI Listing
January 2014

Enhancement of Cerenkov luminescence imaging by dual excitation of Er(3+),Yb(3+)-doped rare-earth microparticles.

PLoS One 2013 25;8(10):e77926. Epub 2013 Oct 25.

Department of Nuclear Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, PR China.

Unlabelled: Cerenkov luminescence imaging (CLI) has been successfully utilized in various fields of preclinical studies; however, CLI is challenging due to its weak luminescent intensity and insufficient penetration capability. Here, we report the design and synthesis of a type of rare-earth microparticles (REMPs), which can be dually excited by Cerenkov luminescence (CL) resulting from the decay of radionuclides to enhance CLI in terms of intensity and penetration.

Methods: Yb(3+)- and Er(3+)- codoped hexagonal NaYF4 hollow microtubes were synthesized via a hydrothermal route. The phase, morphology, and emission spectrum were confirmed for these REMPs by power X-ray diffraction (XRD), scanning electron microscopy (SEM), and spectrophotometry, respectively. A commercial CCD camera equipped with a series of optical filters was employed to quantify the intensity and spectrum of CLI from radionuclides. The enhancement of penetration was investigated by imaging studies of nylon phantoms and nude mouse pseudotumor models.

Results: the REMPs could be dually excited by CL at the wavelengths of 520 and 980 nm, and the emission peaks overlaid at 660 nm. This strategy approximately doubled the overall detectable intensity of CLI and extended its maximum penetration in nylon phantoms from 5 to 15 mm. The penetration study in living animals yielded similar results.

Conclusions: this study demonstrated that CL can dually excite REMPs and that the overlaid emissions in the range of 660 nm could significantly enhance the penetration and intensity of CL. The proposed enhanced CLI strategy may have promising applications in the future.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0077926PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3808356PMC
September 2014

Kinetic modelling and bifurcation analysis of chemomechanically miniaturized gels under mechanical load.

Eur Phys J E Soft Matter 2013 Sep 26;36(9):108. Epub 2013 Sep 26.

State Key Laboratory for Strength and Vibration of Mechanical Structures, School of Aerospace, Xi'an Jiaotong University, 710049, Xi'an, China,

Chemomechanically responsive gels, with great potential applications in the fields of smart structures and biomedicines, present autonomously oscillatory deformation driven by the Belousov-Zhabotinsky chemical reaction. The dynamic behavior of the responsive gels is obviously affected by the external mechanical load. This approach proposed a kinetic model with an ordinary differential equation to describe the oscillatory deformation of the gels under the mechanical load. Then the periodic solutions and phase diagrams of the oscillation are obtained using the improved Runge-Kutta and shooting methods. The results demonstrated that bifurcations are typically existent in the system and the characters of the oscillatory deformation regularly depend on the mechanical load as well as the concentration of reactants and the stoichiometric coefficient of chemical reaction. This development is supposed to promote the practical applications of the chemomechanically responsive gels.
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http://dx.doi.org/10.1140/epje/i2013-13108-xDOI Listing
September 2013

[Regulation of chondrosarcoma cell growth using synthesized hydrogels with different electric charges].

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 2013 Aug;30(4):782-8

MOE Key Laboratory of Biomedical Information Engineering, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.

To develop standard in vitro chondrosarcoma models, we synthesized three hydrogels (i. e., PDMAAm, PNaAMPS and PMETAC) and investigated the influence of Young's modulus, swelling ratio and electric charges on the behavior of chondrosarcoma cells seeded on the hydrogels, including morphology, adhesion and aggregation. Results showed that the morphology of chondrosarcoma cells at 6h was dependent on the charges of hydrogels; cells present spindle-shaped and round-shaped morphology on negative charged and neutral hydrogel, respectively, while no cells spreaded on positive charged hydrogel. Chondrosarcoma cells formed aggregates on neutral PDMAAm after further culture. The hydrogels can be synthesized easily and has the characteristics of ease at use with defined components, which holds great potential for developing standard chondrosarcoma models in vitro.
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August 2013

Potential application of titanium dioxide nanoparticles in the prevention of osteosarcoma and chondrosarcoma recurrence.

J Nanosci Nanotechnol 2013 Feb;13(2):1208-11

The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.

Osteosarcoma and chondrosarcoma are malignant bone tumors, and they significantly affect the life quality of patients including children and adults. The main treatment method is surgical amputation of the malignant lesion, despite that recurrence often occurs. Recently, it has been observed that TiO2 NPs killed HeLa cells effectively via photocatalysis in vitro, which indicates titanium dioxide (TiO2) nanoparticles (NPs) might be used to reduce the recurrence of osteosarcoma and chondrosarcoma by inducing cytotoxicity to bone tumor cells. In this study, we investigated the potential effects of TiO2 NPs in two cancer cell lines in vitro: U-2 OS (osteosarcoma) and SW 1353 (chondrosarcoma). We assessed cell viability, the levels of reactive oxygen species (ROS) and glutathione (GSH) after exposure to TiO2 NPs at different concentrations (0.1-100 microg/ml) for varying exposure periods (12-48 hours). Compared to the NP-free control, TiO2 NPs induced cell death in a dosage-dependent and time-dependent manner. The median inhibitory concentration (IC50) of TiO2 NPs at 24 hours was 211.3 +/- 15.2 microg/ml and 5408.8 +/- 45.9 microg/ml for SW 1353 and U-2 OS cell lines, respectively. TiO2 NPs concentrations above 1 microg/ml were more efficient to reduce the cell viability of SW 1353 than U-2 OS of NPs at all exposure times. The increased ROS and reduced GSH levels indicated that TiO2 NPs killed cancer cells through oxidative stress. These results suggested that the TiO2 NPs can be potentially used to minimize/prevent the recurrence of osteosarcoma and chondrosarcoma.
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http://dx.doi.org/10.1166/jnn.2013.6081DOI Listing
February 2013

Interleukin-6 signaling pathway in targeted therapy for cancer.

Cancer Treat Rev 2012 Nov 29;38(7):904-10. Epub 2012 May 29.

The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, PR China.

Interleukin-6 (IL-6) is a multifunctional cytokine which plays an important role in a wide range of biologic activities in different types of cell including tumor cells. IL-6 is involved in the host immune defense mechanism as well as the modulation of growth and differentiation in various malignancies. These effects are mediated by several signaling pathways, in particular the signal transducer and transcription activator 3 (Stat3). There exists abundant evidence demonstrating that deregulated overexpression of IL-6 was associated with tumor progression through inhibition of cancer cell apoptosis, stimulation of angiogenesis, and drug resistance. Clinical studies have revealed that increased serum IL-6 concentrations in patients are associated with advanced tumor stages of various cancers (e.g., multiple myeloma, non-small cell lung carcinoma, colorectal cancer, renal cell carcinoma, prostate cancer, breast cancer and ovarian cancer) and short survival in patients. Therefore, blocking IL-6 signaling is a potential therapeutic strategy for cancer (i.e., anti-IL-6 therapy) characterized by pathological IL-6 overproduction. Preliminary clinical evidence has shown that antibody targeted IL-6 therapy was well tolerated in cancer patients. In this review, we detail the progress of the current understanding of IL-6 signaling pathway in cancer as well as an antibody targeted IL-6 therapy for human cancer.
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http://dx.doi.org/10.1016/j.ctrv.2012.04.007DOI Listing
November 2012

Recent advances in synthesis and surface modification of lanthanide-doped upconversion nanoparticles for biomedical applications.

Biotechnol Adv 2012 Nov-Dec;30(6):1551-61. Epub 2012 Apr 27.

The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Biomedical Engineering and Biomechanics Center, Department of Chemistry, Xi'an Jiaotong University, Xi'an, PR China.

Lanthanide (Ln)-doped upconversion nanoparticles (UCNPs) with appropriate surface modification can be used for a wide range of biomedical applications such as bio-detection, cancer therapy, bio-labeling, fluorescence imaging, magnetic resonance imaging and drug delivery. The upconversion phenomenon exhibited by Ln-doped UCNPs renders them tremendous advantages in biological applications over other types of fluorescent materials (e.g., organic dyes, fluorescent proteins, gold nanoparticles, quantum dots, and luminescent transition metal complexes) for: (i) enhanced tissue penetration depths achieved by near-infrared (NIR) excitation; (ii) improved stability against photobleaching, photoblinking and photochemical degradation; (iii) non-photodamaging to DNA/RNA due to lower excitation light energy; (iv) lower cytotoxicity; and (v) higher detection sensitivity. Ln-doped UCNPs are therefore attracting increasing attentions in recent years. In this review, we present recent advances in the synthesis of Ln-doped UCNPs and their surface modification, as well as their emerging applications in biomedicine. The future prospects of Ln-doped UCNPs for biomedical applications are also discussed.
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http://dx.doi.org/10.1016/j.biotechadv.2012.04.009DOI Listing
April 2013

Advances in cell-based biosensors using three-dimensional cell-encapsulating hydrogels.

Biotechnol J 2011 Dec;6(12):1466-76

Biomedical Engineering and Biomechanics Center, Xi'an Jiaotong University, Xi'an, P R China.

Cell-based biosensors (CBBs) have emerged as promising biotechnical tools whereby various cell types can be used as basic sensing units to detect external stimuli. Specifically, CBBs have been applied in environmental monitoring, drug screening, clinical diagnosis and biosecurity. For these applications, CBBs offer several advantages over conventional molecular-based biosensors or living animal-based approaches, such as the capability to better mimic physiological situations, to enhance detection specificity and sensitivity, and to detect unknown compounds and toxins. On the other hand, existing CBBs suffer from several limitations, such as weak cell-substrate attachment, two-dimensional (2D) cell microenvironment, and limited shelf life. An emerging method for scaffold-free three-dimensional (3D) cell culture uses hydrogels to encapsulate cells. Advances in novel biomaterials and nano/microscale technologies have enabled encapsulation of cells in hydrogels to fabricate 3D CBBs, which hold great potential for addressing the limitation in existing 2D CBBs. Here, we present an overview of the emerging hydrogel-based CBBs, their applications in pathogen/toxin detection, drug screening and screening of cell-biomaterials interaction, and the associated challenges and potential solutions.
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http://dx.doi.org/10.1002/biot.201100098DOI Listing
December 2011

Thermal shock resistance of skin tissue.

J Med Syst 2011 Oct 7;35(5):863-7. Epub 2010 May 7.

Biomedical Engineering and Biomechanics Center, Xi'an Jiaotong University, Xi'an, People's Republic of China.

Understanding the mechanisms of skin behavior under thermal shock is crucial for medical treatments. However, no reasonable criteria are available for the maximum thermal loadings that skin tissue can survive. To address this, in this paper we analyzed thermal and neural behaviors of skin tissue exposed to thermal loadings by introducing the thermal shock resistance (a parameter widely used for engineering materials) of skin for the first time. Skin thermal shock resistance was analyzed according to two distinct criteria: (1) maximum local temperature at epidermis-dermis (ED) interface defined as the thermal threshold of skin thermal pain; (2) maximum thermal damage at ED interface defined as the first degree burn where irreversible skin damage occurs. Numerical simulation was performed and the results show that the thermal shock resistance of skin tissue depends on the Biot number (which characterizes the features of thermal shock). These results indicate that skin thermal shock resistance can be used as an efficient tool to predict thermal damage (e.g., burn) and the corresponding pain level induced by noxious thermal loadings (e.g., clinical thermal treatments).
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http://dx.doi.org/10.1007/s10916-010-9503-2DOI Listing
October 2011

Multi-scale biothermal and biomechanical behaviours of biological materials.

Philos Trans A Math Phys Eng Sci 2010 Feb;368(1912):517-9

HST Center for Bioengineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

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http://dx.doi.org/10.1098/rsta.2009.0249DOI Listing
February 2010

Effect of initial stress on Love waves in a piezoelectric structure carrying a functionally graded material layer.

Ultrasonics 2010 Jan 23;50(1):84-90. Epub 2009 Aug 23.

Department of Mechanical and Environmental Informatics, Tokyo Institute of Technology, Tokyo 152-8552, Japan.

The effect of initial stress on the propagation behavior of Love waves in a piezoelectric half-space of polarized ceramics carrying a functionally graded material (FGM) layer is analytically investigated in this paper from the three-dimensional equations of linear piezoelectricity. The analytical solutions are obtained for the dispersion relations of Love wave propagating in this kind of structure with initial stress for both electrical open case and electrical short case, respectively. One numerical example is given to graphically illustrate the effect of initial stress on dispersive curve, phase velocity and electromechanical coupling factor of the Love wave propagation. The results reported here are meaningful for the design of surface acoustic wave (SAW) devices with high performance.
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http://dx.doi.org/10.1016/j.ultras.2009.08.011DOI Listing
January 2010

Transverse surface waves in a layered structure with a functionally graded piezoelectric substrate and a hard dielectric layer.

Ultrasonics 2009 Mar 1;49(3):293-7. Epub 2008 Nov 1.

Department of Engineering Mechanics, Xi'an Jiaotong University, Xi'an 710049, Shaanxi Province, PR China.

As to an ideally layered structure with a functionally graded piezoelectric substrate (material parameters change continuously along the thickness direction) and a hard dielectric layer, the existence and propagation behavior of transverse surface waves is studied by analytical technique. The dispersion equations for the existence of the transverse surface waves with respect to phase velocity are obtained for electrically open and short circuit conditions, respectively. A detailed investigation of the effect of gradient coefficient on dispersion relation, electromechanical coupling factor and penetration depth is carried out. It is found by numerical examples that adjusting gradient coefficient makes the electromechanical coupling factor of the transverse surface waves achieve quite high values at some appropriate ratio values of the layer thickness to the wavelength, and at the same time, the penetration depth can be reduced to the same order as the wavelength.
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http://dx.doi.org/10.1016/j.ultras.2008.10.004DOI Listing
March 2009
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