Publications by authors named "Tian-Tian Jiang"

6 Publications

  • Page 1 of 1

Qing-Luo-Yin Alleviated Monocytes/Macrophages-Mediated Inflammation in Rats with Adjuvant-Induced Arthritis by Disrupting Their Interaction with (Pre)-Adipocytes Through PPAR-γ Signaling.

Drug Des Devel Ther 2021 16;15:3105-3118. Epub 2021 Jul 16.

Department of Traditional Chinese Medicine, the First Affiliated Hospital of Wannan Medical College (Yijishan Hospital), Wuhu, 241000, People's Republic of China.

Background: The Chinese herbal formula Qing-Luo-Yin (QLY) has been successfully used in rheumatoid arthritis treatment for decades. It exhibits notable immune and metabolism regulatory properties. Thereby, we investigated its effects on the interplay between (pre)-adipocytes and monocytes/macrophages under adjuvant-induced arthritis (AIA) circumstances.

Methods: Fat reservoir and histological characteristics of white fat tissues (WAT) in AIA rats receiving QLY treatment were examined upon sacrifice. Metabolic parameters, clinical indicators, and oxidative stress levels were determined using corresponding kits, while mRNA/protein expression was investigated by PCR and immunoblotting methods. M1 macrophage distribution in WAT was assessed by flow cytometry. The effects of QLY on (pre)-adipocytes were further validated by experiments in vitro.

Results: Compared with normal healthy controls, body weight and circulating triglyceride were declined in AIA rats, but serological levels of free fatty acids and low-density lipoprotein cholesterol were increased. mRNA IL-1β and iNOS expression in white blood cells and rheumatoid factor, C-reactive protein, anti-cyclic citrullinated peptide antibody, MCP-1 and IL-1β production in serum/WAT were up-regulated. Obvious CD86CD11b macrophages were enriched in WAT. Meanwhile, expression of PPAR-γ and SIRT1 and secretion of adiponectin and leptin in these AIA rats were impaired. QLY restored all these pathological changes. Of note, it significantly stimulated PPAR-γ expression in the treated AIA rats. Accordingly, QLY-containing serum promoted SCD-1, PPAR-γ, and SIRT1 expression in pre-adipocytes cultured in vitro. AIA rats-derived peripheral blood mononuclear cells suppressed PPAR-γ and SCD-1 expression in co-cultured pre-adipocytes, but serum from AIA rats receiving QLY treatment did not exhibit this potential. The changes on PPAR-γ expression eventually resulted in varied adipocyte differentiation statuses. PPAR-γ selective inhibitor T0070907 abrogated QLY-induced MCP-1 production decline in LPS-primed pre-adipocytes and reduced adiponectin secretion.

Conclusion: QLY was potent in promoting PPAR-γ expression and consequently disrupted inflammatory feedback in WAT by altering monocytes/macrophages polarization and adipocytes differentiation.
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http://dx.doi.org/10.2147/DDDT.S320599DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8291661PMC
July 2021

Nicotinamide mononucleotide-elicited NAMPT signaling activation aggravated adjuvant-induced arthritis in rats by affecting peripheral immune cells differentiation.

Int Immunopharmacol 2021 Jun 12;98:107856. Epub 2021 Jun 12.

Department of Traditional Chinese Medicine, the First Affiliated Hospital of Wannan Medical College (Yijishan Hospital), Wuhu 241000, China; Research Center of Integration of Traditional Chinese and Western Medicine, Wannan Medical College, Wuhu 241000, China; Key Laboratory of Non-coding RNA Transformation Research of Anhui Higher Education Institution, Wannan Medical College, Wuhu 241000, China. Electronic address:

Supplement of nicotinamide mononucleotide (NMN), the direct precursor of nicotinamide adenine dinucleotide (NAD) has gained prominence due to the significant anti-aging potentials of nicotinamide phosphoribosyltransferas (NAMPT)/NAD signaling. Because over-expression of NAMPT is deeply implicated in inflammatory arthritis, we investigated the effects of NMN supplement on rats with adjuvant-induced arthritis (AIA). Tested rats were given oral treatment of NMN at 200 mg/kg/day for 25 days. Arthritis score and body weight were periodically recorded. Clinical outcomes were evaluated based on arthritic manifestations, ELISA analysis and histological examination. T cells subsets were analyzed by flow cytometry. Expressions of protein and mRNA were assessed by immunoblotting and PCR methods, respectively. Levels of CD172a, CD43, and NAMPT in peripheral blood mononuclear cells (PBMCs) were investigated by immunofluorescence approach. Obtained results were further validated by experiments in vitro. Generally, NMN exacerbated AIA severity in rats. It deteriorated MMP3-controlled tissues damages, and altered immune profile by increasing Th17/Treg cells ratio. The up-regulation of NAMPT in PBMCs from NMN-treated rats was confirmed by both immunofluorescence and PCR experiments, which was synchronized with significant increase in iNOS, MCP-1, IL-1β expression. NMN-primed AIA PBMCs were potent in up-regulating MCP-1, IL-1β, MMP3 and p-JNK expression in synovioblast. NMN stimulus barely affected Th17 cells count in in vitro cultured splenocytes, but it greatly potentiated the capability of AIA monocytes in inducing IL-17α secretion and Th17 cells differentiation in the co-cultured splenocytes. It suggested that long-term NMN supplement could exacerbate inflammatory arthritis by reshaping the immune milieu through the up-regulation of NAMPT.
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http://dx.doi.org/10.1016/j.intimp.2021.107856DOI Listing
June 2021

-Derived Xanthones Protected Joints from Degradation in Male Rats with Collagen-Induced Arthritis by Regulating PPAR-γ Signaling.

J Inflamm Res 2021 16;14:395-411. Epub 2021 Feb 16.

School of Pharmacy, Anhui College of Traditional Chinese Medicine, Wuhu, 241000, Anhui, People's Republic of China.

Background: The bark of Hassk. is traditionally used for treating inflammatory diseases and bone fractures in China. We have previously validated the xanthone-enriched fraction (XRF) of with anti-rheumatic potentials, but mechanism underlying the joints protective effects is still largely unknown.

Materials And Methods: The male rats with collagen-induced arthritis (CIA) were treated with XRF. The therapeutic efficacy of XRF was evaluated by arthritis score changes, morphological observation of paws, histological examinations and serological analyses. Protein expression in tissues and cells was investigated by either immunohistochemical or immunoblotting methods, while levels of mRNA expression were investigated by RT-qPCR. Metabolites in serum were detected by LC-MS approach. The joints homogenates were used for analyzing possible targeted genes by genome microarray analyses.

Results: Treatment with XRF and methotrexate (MTX) led to significant decrease in arthritis scores, and alleviated deformation of paws in CIA rats. In addition, XRF and MTX reduced circulating TNF-α, IL-1β and IL-17α in the serum and down-regulated TLR4/NF-κB and JNK pathways in joints of CIA rats. Compared to MTX, XRF-loading microemulsion significantly protected joints, which was accompanied by dramatic decrease in MMP3. Differential genes-based KEGG enrichment and metabolomics analysis suggested that XRF reduced fatty acids biosynthesis by regulating PPAR-γ signaling. -derived 1,7-dihydroxy-3,4-dimethoxyxanthone (XAN) up-regulated PPAR-γ expression in macrophages, but suppressed it in pre-adipocytes in vitro, which was synchronized with SIRT1 changes. Adiponectin production and SCD-1 expression in pre-adipocytes were also decreased. Aside the direct inhibition on MMP3 expression in synovioblast, the presence of XAN in macrophages-pre-adipocytes co-culture system further reinforced this effect.

Conclusion: This study revealed the joint protective  advantages of the bioactive fraction from in CIA rats over MTX, and demonstrated that -derived xanthones suppressed the erosive nature of synovioblast acquired under inflammatory circumstances by regulating PPAR-γ signaling-controlled metabolism-immunity feedback.
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http://dx.doi.org/10.2147/JIR.S295957DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7896782PMC
February 2021

The occurrence and spatial-temporal distribution of tetrabromobisphenol A in the coastal intertidal zone of Qingdao in China, with a focus on toxicity assessment by biological monitoring.

Chemosphere 2017 Oct 10;185:462-467. Epub 2017 Jul 10.

Laboratory for Plankton, College of Marine Life Sciences, Ocean University of China, Qingdao, Shandong, China.

Tetrabromobisphenol A (TBBPA) is a widely used flame retardant that has increasingly been found as contaminant in aquatic environments. The main goal of this study was to evaluate the pollution level of TBBPA at six locations around Qingdao and assess its biotoxicity through a two-generation toxicity study looking at a copepod species. In the chemical monitoring, the concentration of TBBPA in seawater samples ranged from nd to 1.8 μg/L. Next, the biological indicator monitoring used 1.8 μg/L as the middle exposure concentration to perform quantitative evaluations of the influence of TBBPA on the demographic traits of Pseudodiaptomus inopinus. The results showed that copepods became more sensitive to TBBPA exposure even in environmental concentration (1.8 μg/L) as the generations developed. The detrimental effects of TBBPA further increased naupliar mortality and impaired copepodite development to adulthood. This study demonstrated that the water pollution condition of TBBPA was measured at all 6 sampling locations of Qingdao. Therefore, the present results call for a decreased discharge of TBBPA into the marine environment to avoid impairing copepod reproduction and development.
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http://dx.doi.org/10.1016/j.chemosphere.2017.07.033DOI Listing
October 2017

[Occurrence of PFOA and PFOS in the aquatic environments and their ecological toxicities in the aquatic environments].

Huan Jing Ke Xue 2011 Oct;32(10):2817-26

Laboratory of Water Environmental System Engineering, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.

Based on current researches, described the sources, toxicities, analytical methods and occurrence of PFOA and PFOS in wastewater treatment plants, lakes, rivers, coastal areas and tap waters in different countries in the world. According to the limited concentrations of PFOA + PFOS in effluents of wastewater treatment plants (WTPs) put forward by Germany, the concentrations of PFOA + PFOS in WTP effluents in Singapore, Germany, Korea and England were assessed. The results showed that the concentrations of PFOA + PFOS in WTP effluents in Singapore were higher than the liminted concentration of 300 microg/L. Researches done by China, Japan, USA, and other countries showed that the concentrations of POFA and PFOS in rivers and coastal areas reached to ng/L, and the concentrations in Tennessee River, USA were the highest, reached to 100 ng/L. The risk assessments of PFOA and PFOS in tap water in some cities in China were assessed according to the risk quotients(RQ). The assessment results showed that tap waters from 19 cities in China were all below the risk level.
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October 2011

[Study on ecoregion techniques of lake nutrients in Yunnan-Guizhou plateau Lake regions].

Huan Jing Ke Xue 2010 Nov;31(11):2599-606

Chinese Research Academy of Environmental Sciences, Beijing 100012, China.

The primary objective of this research is to present an ecoregion model based on the principle components analysis (PCA), the cluster analysis (CA), the discriminant analysis (DA) and the spatial autocorrelation analysis (SAA), which were applied to establish the ecorigion of lake nutrients in Yunnan-Guizhou Plateau Lakes. First, the principle components analysis method was used not only to reduce the dimensionality of the feature space, but also to deal with new indexes. The accumulation contribution ratio of the four new indexes achieved 93.69%, which had the full representation. These complementary indexes which reflect the conditions of lakes were helpful to establish the index system of ecorigion of lake nutrients in Yunnan-Guizhou Plateau Lakes. Second, initial classification of lake drainage area was accomplished by new indicator values and cluster analysis which contained five classes, and then the categories of non-lake drainage area could be identified by the discriminant analysis. Finally, the cluster result was carried on the global statistics, Moran's I was 0.32 which was higher than 0, and the examination value Z was 68.28 which was higher than marginal value (remarkable level 1% correspond 2.58), it was indicated that the cluster result and the space position had the remarkable relativity, afterward spatial distribution pattern of the region was achieved by the quantificational analysis about over score of principle components and application of local spatial autocorrelation method. Then the ecorigion of lake nutrients was accomplished by the association and difference between different districts of the scattered regions' s spatial character. These results indicated that the ecoregion model initiated in the paper was appropriate and feasible to receive objective results and avoid artificial factors. Also it has provided a new method to explore the index system and techniques about the ecoregion of lake nutrients. Scientifically, the model was in favor of the foundation of lake nutrient criteria and eutrophication control standards in China.
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November 2010
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