Publications by authors named "Tian Tian"

1,234 Publications

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Immunogenic Cell Death Augmented by Manganese Zinc Sulfide Nanoparticles for Metastatic Melanoma Immunotherapy.

ACS Nano 2022 Aug 18. Epub 2022 Aug 18.

Guangdong Provincial Key Laboratory of Digestive Cancer Research and The Seventh Affiliated Hospital of Sun Yat-Sen University, Shenzhen, Guangdong 518107, P. R. China.

Both T-cell deprivation and insufficient tumor immunogenicity seriously hinder the efficacy of immune-mediated tumor destruction in melanoma. In this work, an amphiphilic polyethylene glycol-poly(2-hexoxy-2-oxo-1,3,2-dioxaphospholane) copolymer with a thermally sensitive flowable core (mPEG--PHEP) was chosen to incorporate IR780 dye and manganese zinc sulfide nanoparticles (ZMS) to form polymer micelles (denoted PP), which precisely controlled the release of ZMS after being triggered by near-infrared light (NIR). Mn-mediated chemodynamic therapy (CDT) by photothermal trigger boosted the generation of reactive oxygen species (ROS), making the PP smart bomblets . It was demonstrated that PP could maximize immunogenic cell death (ICD) in cancer, which is characterized by abundant damage-associated molecular pattern (DAMP) exposure. As a result, the cytotoxic T cells (CD8) and helper T cells (CD4) expanded and infiltrated the neoplastic foci, which further reprogrammed the suppressive tumor microenvironment (TME) against the primary tumor and pulmonary metastases with safe systemic cytokine expression. In addition, Mn-mediated cGAS-STING signaling pathway activation enhanced the antitumor immunity of this nanocomposite, providing a practical strategy for expanding the use of Mn-based nanostructures.
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http://dx.doi.org/10.1021/acsnano.2c08013DOI Listing
August 2022

COVID-19 vaccination perception and uptake among cancer patients in Guangzhou, China.

Hum Vaccin Immunother 2022 Aug 17:2102329. Epub 2022 Aug 17.

School of Public Health (Shenzhen), Sun Yat-sen University, Shenzhen, China.

Patients with cancer are considered at high risk of COVID-19 related complications with higher mortality rates than healthy individuals. This study investigated the perception, acceptance, and influencing factors of COVID-19 vaccination among cancer patients in Guangzhou, China. A cross-sectional survey was conducted in Guangzhou, China from August to November 2021 in two tertiary medical centers. Outpatients were recruited through hospital posters to complete a questionnaire including demographics, medical history, knowledge, and attitude toward COVID-19 vaccines and COVID-19 vaccination status. Chi-square tests and multivariable logistic regression were used to analyze predictors for acceptance of COVID-19 vaccination. In total, only 75 out of 343 patients (21.87%) had received at least one dose of COVID-19 vaccine. Twenty-one patients (6.12%) had received a recommendation about COVID-19 vaccination from their physicians. Patients who were recommended by physicians to get vaccinated (aOR = 11.71 95% CI: 2.71-50.66), with a monthly income of more than CNY 5000 (aOR = 3.94, 95% CI: 1.88-8.26) were more likely to have received COVID-19 vaccination. Cancer patients who had been diagnosed for more than one year (aOR = 0.21, 95% CI: 0.09-0.51), had received multiple cancer treatment strategies (aOR = 0.34, 95% CI: 0.16-0.74), worried about the safety of COVID-19 vaccines (aOR = 0.21, 95% CI: 0.11-0.40), were less likely to have received COVID-19 vaccination. COVID-19 vaccination uptake among cancer patients was insufficient. The proportion of cancer patients receiving vaccination recommendations from physicians remains inadequate. Physicians are expected to play an essential role in patients' knowledge of the safety and effectiveness of COVID-19 vaccines.
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http://dx.doi.org/10.1080/21645515.2022.2102329DOI Listing
August 2022

Discovery and validation of tissue-specific DNA methylation as noninvasive diagnostic markers for colorectal cancer.

Clin Epigenetics 2022 Aug 16;14(1):102. Epub 2022 Aug 16.

Department of Epidemiology, School of Public Health, Harbin Medical University, Harbin, China.

Background: Noninvasive diagnostic markers that are capable of distinguishing patients with colorectal cancer (CRC) from healthy individuals or patients with other cancer types are lacking. We report the discovery and validation of a panel of methylation-based markers that specifically detect CRC.

Methods: This was a large-scale discovery study based on publicly available datasets coupled with a validation study where multiple types of specimens from six cohorts with CRC, other cancer types, and healthy individuals were used to identify and validate the tissue-specific methylation patterns of CRC and assess their diagnostic performance.

Results: In the discovery and validation cohort (N = 9307), ten hypermethylated CpG sites located in three genes, C20orf194, LIFR, and ZNF304, were identified as CRC-specific markers. Different analyses have suggested that these CpG sites are CRC-specific hypermethylated and play a role in transcriptional silencing of corresponding genes. A random forest model based on ten markers achieved high accuracy rates between 85.7 and 94.3% and AUCs between 0.941 and 0.970 in predicting CRC in three independent datasets and a low misclassification rate in ten other cancer types. In the in-house validation cohort (N = 354), these markers achieved consistent discriminative capabilities. In the cfDNA pilot cohort (N = 14), hypermethylation of these markers was observed in cfDNA samples from CRC patients. In the cfDNA validation cohort (N = 155), the two-gene panel yielded a sensitivity of 69.5%, specificity of 91.7%, and AUC of 0.806.

Conclusions: Hypermethylation of the ten CpG sites is a CRC-specific alteration in tissue and has the potential use as a noninvasive cfDNA marker to diagnose CRC.
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http://dx.doi.org/10.1186/s13148-022-01312-9DOI Listing
August 2022

Assessing analgesic efficacy of glossopharyngeal nerve block after oropharyngeal surgery.

Am J Otolaryngol 2022 Aug 9:103600. Epub 2022 Aug 9.

Department of Anesthesiology, Beijing Friendship Hospital, Capital Medical University, Beijing, People's Republic of China.

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http://dx.doi.org/10.1016/j.amjoto.2022.103600DOI Listing
August 2022

The nomograms to predict early death among metastatic small-cell lung cancer patients: a retrospective study based on SEER database.

Transl Cancer Res 2022 Jul;11(7):2122-2134

Cancer Center, West China Hospital, Sichuan University, Chengdu, China.

Background: This study aims to discriminate risk factors associated with early death (died within 3 months) in metastatic small-cell lung cancer (SCLC) patients, and construct predictive nomograms to help physicians in guiding individual treatment.

Methods: Surveillance, Epidemiology, and End Results (SEER) database was used to obtain records of deceased metastatic SCLC patients. The univariate and multivariate logistic regression methods were managed to identify risk factors for early death in overall patients and chemotherapy recipients. Predictive nomograms were developed and then validated by receiver operating characteristics curve (ROC) and calibration plots to verify its' precision.

Results: A total of 13,229 patients were collected of which 5,832 of them encountered early death. The univariate and multivariate logistic regression analysis identified variables that were negatively associated with early death include sex, age, race, sequence, T stage, N stage, organ metastasis. Chemotherapy and radiotherapy implementation significantly decreased the odds of early death. For the chemotherapy recipients, white male patients with advanced age (over 80 years old), T4 stage, multiple organ metastasis, and without radiotherapy most likely died within 3 months. The area under the curve (AUC) of the nomograms for overall population and chemotherapy recipients' early death prediction was 0.839 and 0.653.

Conclusions: Early death among metastatic SCLC patients was extremely common in clinical practice. The nomograms constructed were able to assist clinical physicians in discriminating high-risk SCLC patients for targeted intervention, and elderly white male patients diagnosed with advanced T stage and multiple organ metastasis might be exempted from systemic treatment to receive palliative care.
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http://dx.doi.org/10.21037/tcr-22-462DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9372205PMC
July 2022

Diagnosing Diabetic Retinopathy in OCTA Images Based on Multilevel Information Fusion Using a Deep Learning Framework.

Comput Math Methods Med 2022 4;2022:4316507. Epub 2022 Aug 4.

Faculty of Information Technology, Beijing University of Technology, Beijing 100124, China.

Objective: As an extension of optical coherence tomography (OCT), optical coherence tomographic angiography (OCTA) provides information on the blood flow status at the microlevel and is sensitive to changes in the fundus vessels. However, due to the distinct imaging mechanism of OCTA, existing models, which are primarily used for analyzing fundus images, do not work well on OCTA images. Effectively extracting and analyzing the information in OCTA images remains challenging. To this end, a deep learning framework that fuses multilevel information in OCTA images is proposed in this study. The effectiveness of the proposed model was demonstrated in the task of diabetic retinopathy (DR) classification.

Method: First, a U-Net-based segmentation model was proposed to label the boundaries of large retinal vessels and the foveal avascular zone (FAZ) in OCTA images. Then, we designed an isolated concatenated block (ICB) structure to extract and fuse information from the original OCTA images and segmentation results at different fusion levels.

Results: The experiments were conducted on 301 OCTA images. Of these images, 244 were labeled by ophthalmologists as normal images, and 57 were labeled as DR images. An accuracy of 93.1% and a mean intersection over union (mIOU) of 77.1% were achieved using the proposed large vessel and FAZ segmentation model. In the ablation experiment with 6-fold validation, the proposed deep learning framework that combines the proposed isolated and concatenated convolution process significantly improved the DR diagnosis accuracy. Moreover, inputting the merged images of the original OCTA images and segmentation results further improved the model performance. Finally, a DR diagnosis accuracy of 88.1% (95%CI ± 3.6%) and an area under the curve (AUC) of 0.92 were achieved using our proposed classification model, which significantly outperforms the state-of-the-art classification models. As a comparison, an accuracy of 83.7 (95%CI ± 1.5%) and AUC of 0.76 were obtained using EfficientNet. . The visualization results show that the FAZ and the vascular region close to the FAZ provide more information for the model than the farther surrounding area. Furthermore, this study demonstrates that a clinically sophisticated designed deep learning model is not only able to effectively assist in the diagnosis but also help to locate new indicators for certain illnesses.
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http://dx.doi.org/10.1155/2022/4316507DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9371870PMC
August 2022

Adsorption performance and optimization by response surface methodology on tetracycline using Fe-doped ZIF-8-loaded multi-walled carbon nanotubes.

Environ Sci Pollut Res Int 2022 Aug 13. Epub 2022 Aug 13.

School of Civil Engineering, Nanjing Forestry University, Longpan Road 159#, Nanjing, 210037, China.

Herein, an iron-doped ZIF-8-loaded multi-walled carbon nanotube (FZM) was synthesized and its adsorption performance on tetracycline (TC) was investigated. The experimental conditions (solution pH, temperature, adsorbent dose) were optimized by Box-Behnken design (BBD) in response surface methodology (RSM). The results show that the adsorption effect of TC by FZM is optimal under the conditions of temperature = 298 K, pH = 6, and contact time = 360 min. The adsorption processes of TC by FZM follow the pseudo-second-order (PSO) kinetic and Freundlich isotherm models, indicating that chemisorption is the dominant factor and the adsorption reaction is multi-layer, with a theoretical maximum saturation capacity of 1111.11 mg/g at 298 K. The adsorption thermodynamic results indicate that the adsorption of TC by FZM is a spontaneous and endothermic process. The mechanism of TC adsorption by FZM possibly occurs through hydrogen bonding, surface complexation, π-π interaction, and electrostatic interaction. From the statistical results, the optimal adsorption capacity of TC by FZM is 599.78 mg/g at a pH of 7.1, a temperature of 312.5 K, and an adsorbent dose of 64.43 mg/L, with a deviation of 1.73% from the actual value. Furthermore, regeneration experiments demonstrate that FZM has excellent reusability with a 15% loss of adsorption capacity after four cycles. This study provides some insights to study the adsorption behavior of TC by MOFs and the optimization of the adsorption experimental conditions, and also shows the potential of FZM for TC removal.
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http://dx.doi.org/10.1007/s11356-022-22524-9DOI Listing
August 2022

The trophectoderm acts as a niche for the inner cell mass through C/EBPα-regulated IL-6 signaling.

Stem Cell Reports 2022 Aug 4. Epub 2022 Aug 4.

Gene Regulation, Stem Cells and Cancer Program, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology (BIST), Barcelona, Spain; Universitat Pompeu Fabra (UPF), Barcelona, Spain. Electronic address:

IL-6 has been shown to be required for somatic cell reprogramming into induced pluripotent stem cells (iPSCs). However, how Il6 expression is regulated and whether it plays a role during embryo development remains unknown. Here, we describe that IL-6 is necessary for C/EBPα-enhanced reprogramming of B cells into iPSCs but not for B cell to macrophage transdifferentiation. C/EBPα overexpression activates both Il6 and Il6ra genes in B cells and in PSCs. In embryo development, Cebpa is enriched in the trophectoderm of blastocysts together with Il6, while Il6ra is mostly expressed in the inner cell mass (ICM). In addition, Il6 expression in blastocysts requires Cebpa. Blastocysts secrete IL-6 and neutralization of the cytokine delays the morula to blastocyst transition. The observed requirement of C/EBPα-regulated IL-6 signaling for pluripotency during somatic cell reprogramming thus recapitulates a physiologic mechanism in which the trophectoderm acts as niche for the ICM through the secretion of IL-6.
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http://dx.doi.org/10.1016/j.stemcr.2022.07.009DOI Listing
August 2022

A Genome-Wide Analysis of Genes Reveals the Critical Role in Enhanced Bacterial Wilt Tolerance in Potato During Infection.

Front Genet 2022 26;13:894844. Epub 2022 Jul 26.

College of Life Sciences, Shanxi Normal University, Taiyuan, China.

is one of the members of TGACG sequence-specific binding protein family, which plays a crucial role in the regulated course of hormone synthesis as a stress-responsive transcription factor (TF). Little is known, however, about its implication in response to bacterial wilt disease in potato () caused by . Here, we performed an identification and analysis of the members of the family based on the whole genome data of potato. In total, 42 were predicted to be distributed on four chromosomes in potato genome. Phylogenetic analysis showed that the proteins of could be divided into six sub-families. We found that many of these genes have more than one exon according to the conserved motif and gene structure analysis. The heat map inferred that are generally expressed in different tissues which are at different stages of development. Genomic collinear analysis showed that there are homologous relationships among potato, tomato, pepper, Arabidopsis, and tobacco genes. Cis-element analysis predicted that there may be many cis-acting elements related to abiotic and biotic stress upstream of promoter including plant hormone response elements. A representative member was selected to investigate the potential function of the genes for further analysis. Quantitative real-time polymerase chain reaction (qRT-PCR) assays indicated that the expression of the was significantly induced by infection and upregulated by exogenous salicylic acid (SA), abscisic acid (ABA), gibberellin 3 (GA), and methyl jasmonate (MeJA). The results of yeast one-hybrid (Y1H) assay showed that regulates BRI1-associated receptor kinase 1 () expression. Thus, our study provides a theoretical basis for further research of the molecular mechanism of the gene of potato tolerance to bacterial wilt.
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http://dx.doi.org/10.3389/fgene.2022.894844DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9360622PMC
July 2022

Transition Metal Nitrides for Electrocatalytic Application: Progress and Rational Design.

Nanomaterials (Basel) 2022 Aug 3;12(15). Epub 2022 Aug 3.

Foshan Xianhu Laboratory of the Advanced Energy Science and Technology Guangdong Laboratory, Xianhu Hydrogen Valley, Foshan 528200, China.

The energy crisis and environmental issues are becoming more severe due to the long-term consumption of fossil fuels. Therefore, novel energy-conversion devices with high energy density and environmental friendliness are expected to provide reliable alternatives to traditional fossil-based energy systems. However, because of the inevitable use of costly precious metals as the electrode catalysts for such devices, their popularization is seriously hindered. Transition metal nitrides (TMNs) exhibit similar surface and adsorption properties to noble metals because the atomic distance between metal atoms increases and the d-band center of metal atoms downshifts after nitrogen atoms enter the metal lattice. TMNs have become one of the best electrode materials to replace noble metal-based electrocatalysts in next-generation energy-storage and energy-conversion devices. In this review, the recent developments in the electrocatalytic application of TMNs are covered. First, we discuss the structure and activity origin of TMNs and introduce the common synthesis methods for the preparation of TMNs. Subsequently, we illustrate the applications of mono-metallic TMNs and multi-metallic TMNs in oxygen-reduction reaction, oxygen-evolution reaction, and bifunctional oxygen reduction and evolution reactions. Finally, we summarize the challenges of TMNs encountered at the present stage, and expect their future development.
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http://dx.doi.org/10.3390/nano12152660DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9370505PMC
August 2022

Recent Advances Regarding Precious Metal-Based Electrocatalysts for Acidic Water Splitting.

Nanomaterials (Basel) 2022 Jul 29;12(15). Epub 2022 Jul 29.

Foshan Xianhu Laboratory, Advanced Energy Science and Technology Guangdong Laboratory, Xianhu Hydrogen Valley, Foshan 528200, China.

Electrochemical water splitting has wide applicability in preparing high-density green energy. The Proton exchange membrane (PEM) water electrolysis system is a promising technique for the generation of hydrogen due to its high electrolytic efficiency, safety and reliability, compactness, and quick response to renewable energy sources. However, the instability of catalysts for electrochemical water splitting under operating conditions limits their practical applications. Until now, only precious metal-based materials have met the requirements for rigorous long-term stability and high catalytic activity under acid conditions. In this review, the recent progress made in this regard is presented and analyzed to clarify the role of precious metals in the promotion of the electrolytic decomposition of water. Reducing precious metal loading, enhancing catalytic activity, and improving catalytic lifetime are crucial directions for developing a new generation of PEM water electrolysis catalysts. A summary of the synthesis of high-performance catalysts based on precious metals and an analysis of the factors affecting catalytic performance were derived from a recent investigation. Finally, we present the remaining challenges and future perspectives as guidelines for practical use.
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http://dx.doi.org/10.3390/nano12152618DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9370661PMC
July 2022

SENP1-KLF4 Signaling Regulates LPS-induced Macrophage M1 Polarization.

FEBS J 2022 Aug 9. Epub 2022 Aug 9.

Department of Ophthalmology, Xinhua Hospital, Affiliated to Medicine School of Shanghai Jiaotong University, Shanghai, China.

Macrophages are very important immune cells and play critical roles in tumor immunity. Macrophage subtypes can be divided into classical polarization (M1 macrophages) and alternative polarization (M2 macrophages) under different microenvironments. Krüppel-like factor 4 (KLF4) is an essential transcription factor for macrophage polarization. Our previous study has shown that KLF4 SUMOylation plays an important role in macrophage M2 polarization. In this study, SENP1 was identified as a specific protease for KLF4 de-SUMOylation, with the SENP1-KLF4 axis playing a vital role in M1 macrophage polarization by affecting the NF-κB signaling pathway. Additionally, the activity of tumor cells was weakened by KLF4 SUMOylation deficient macrophages. Hence, the SENP1-KLF4 axis is considered to play a crucial role in regulating LPS-induced macrophage M1 polarization, thereby affecting the activity of tumor cells. Therefore, the SENP1-KLF4 axis has therapeutic potential as a target in cancer therapy.
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http://dx.doi.org/10.1111/febs.16589DOI Listing
August 2022

Regarding "Preoperative Quadratus Lumborum Block Reduces Opioid Requirements in the Immediate Postoperative Period Following Hip Arthroscopy: A Randomized, Blinded Clinical Trial".

Arthroscopy 2022 08;38(8):2365-2366

Department of Anesthesiology, Beijing Friendship Hospital, Capital Medical University, Beijing, People's Republic of China.

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http://dx.doi.org/10.1016/j.arthro.2022.06.002DOI Listing
August 2022

Assessing of analgesic efficacy of preemptive local anesthesia following vaginal hysterectomy.

Eur J Obstet Gynecol Reprod Biol 2022 Aug 3. Epub 2022 Aug 3.

Department of Anesthesiology, Beijing Friendship Hospital, Capital Medical University, Beijing, People's Republic of China.

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http://dx.doi.org/10.1016/j.ejogrb.2022.07.033DOI Listing
August 2022

Nanotechnology-Inspired Extracellular Vesicles Theranostics for Diagnosis and Therapy of Central Nervous System Diseases.

ACS Appl Mater Interfaces 2022 Aug 5. Epub 2022 Aug 5.

Experimental Therapeutics and Molecular Imaging Unit, Department of Neurology, Neuro-Oncology Division, Massachusetts General Hospital, Boston, Massachusetts 02129, United States.

Shuttling various bioactive substances across the blood-brain barrier (BBB) bidirectionally, extracellular vesicles (EVs) have been opening new frontiers for the diagnosis and therapy of central nervous system (CNS) diseases. However, clinical translation of EV-based theranostics remains challenging due to difficulties in effective EV engineering for superior imaging/therapeutic potential, ultrasensitive EV detection for small sample volume, as well as scale-up and standardized EV production. In the past decade, continuous advancement in nanotechnology provided extensive concepts and strategies for EV engineering and analysis, which inspired the application of EVs for CNS diseases. Here we will review the existing types of EV-nanomaterial hybrid systems with improved diagnostic and therapeutic efficacy for CNS diseases. A summary of recent progress in the incorporation of nanomaterials and nanostructures in EV production, separation, and analysis will also be provided. Moreover, the convergence between nanotechnology and microfluidics for integrated EV engineering and liquid biopsy of CNS diseases will be discussed.
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http://dx.doi.org/10.1021/acsami.2c07981DOI Listing
August 2022

Application of fluoride disturbs plaque microecology and promotes remineralization of enamel initial caries.

J Oral Microbiol 2022 27;14(1):2105022. Epub 2022 Jul 27.

Department of Operative Dentistry & Endodontics, State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Shaanxi Key Laboratory of Stomatology, School of Stomatology, the Fourth Military Medical University, Xi'an, PR China.

Background: The caries-preventive effect of topical fluoride application has been corroborated by a number of clinical studies. However, the effect of fluoride on oral microecology remains unclear.

Objective: To monitor the effect of fluoride on dental plaque microecology and demineralization/remineralization balance of enamel initial caries.

Methods: Three-year-old children were enrolled and treated with fluoride at baseline and 6 months. International Caries Detection and Assessment System II indices of 52 subjects were measured at baseline, 3, 6, and 12 months. Supragingival plaque samples of 12 subjects were collected at baseline, 3 and 14 days for 16S rRNA sequencing.

Results: Changes in microbial community structure were observed at 3 days after fluoridation. Significant changes in the relative abundance of microorganisms were observed after fluoride application, especially , unidentified and . Functional prediction revealed that cell movement, carbohydrate and energy metabolism were affected significantly after fluoride application. Fluoride significantly inhibited enamel demineralization and promoted remineralization of early demineralized caries enamel at 3 months.

Conclusion: Fluoride application significantly inhibited the progression of enamel initial caries and reversed the demineralization process, possibly by disturbing dental plaque microecology and modulating the physicochemical action of demineralization/remineralization. This deepened our understanding of caries-preventive effects and mechanisms of fluoride.
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http://dx.doi.org/10.1080/20002297.2022.2105022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9341347PMC
July 2022

Variable selection for nonparametric additive Cox model with interval-censored data.

Biom J 2022 Aug 3. Epub 2022 Aug 3.

Department of Statistics, University of Missouri, Columbia, USA, MO.

The standard Cox model is perhaps the most commonly used model for regression analysis of failure time data but it has some limitations such as the assumption on linear covariate effects. To relax this, the nonparametric additive Cox model, which allows for nonlinear covariate effects, is often employed, and this paper will discuss variable selection and structure estimation for this general model. For the problem, we propose a penalized sieve maximum likelihood approach with the use of Bernstein polynomials approximation and group penalization. To implement the proposed method, an efficient group coordinate descent algorithm is developed and can be easily carried out for both low- and high-dimensional scenarios. Furthermore, a simulation study is performed to assess the performance of the presented approach and suggests that it works well in practice. The proposed method is applied to an Alzheimer's disease study for identifying important and relevant genetic factors.
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http://dx.doi.org/10.1002/bimj.202100310DOI Listing
August 2022

BCHE as a Prognostic Biomarker in Endometrial Cancer and Its Correlation with Immunity.

J Immunol Res 2022 21;2022:6051092. Epub 2022 Jul 21.

Department of Obstetrics and Gynecology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China.

Background: In developed countries, the most common gynecologic malignancy is endometrial carcinoma (EC), making the identification of EC biomarkers extremely essential. As a natural enzyme, butyrylcholinesterase (BCHE) is found in hepatocytes and plasma. There is a strong correlation between BCHE gene mutations and cancers and other diseases. The aim of this study was to analyze the role of BCHE in patients with EC.

Methods: A variety of analyses were conducted on The Cancer Genome Atlas (TCGA) data, including differential expression analysis, enrichment analysis, immunity, clinicopathology, and survival analysis. The Gene Expression Omnibus (GEO) database was used to validate outcomes. Using R tools, Gene Set Enrichment Analysis (GSEA) and Gene Ontology (GO) analyses revealed the potential mechanisms of BCHE in EC. Sangerbox tools were used to delve into the relations between BCHE expression and tumor microenvironment, including microsatellite instability (MSI), tumor neoantigen count (TNC), and tumor mutation burden (TMB). BCHE's genetic alteration analysis was conducted by cBioPortal. In addition, the Human Protein Atlas (HPA) was used to validate the outcomes by immunohistochemistry, and an analysis of the protein-protein interaction network (PPI) was performed with the help of the STRING database.

Results: Based on our results, BCHE was a significant independent prognostic factor for patients with EC. The prognosis with EC was affected by age, stage, grade, histological type, and BCHE. GSEA showed that BCHE was closely related to pathways regulating immune response, including transforming growth factor- (TGF-) signaling pathways and cancer immunotherapy through PD1 blockade pathways. The immune analysis revealed that CD4+ regulatory T cells (Tregs) were negatively correlated with BCHE expression and the immune checkpoint molecules CD28, ADORA2A, BTNL2, and TNFRSF18 were all significantly related to BCHE. BCHE expression was also associated with TMB by genetic alteration analysis.

Conclusions: Identifying BCHE as a biomarker for EC might help predict its prognosis and could have important implications for immunotherapy.
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http://dx.doi.org/10.1155/2022/6051092DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338740PMC
August 2022

Identification of early invisible acute ischemic stroke in non-contrast computed tomography using two-stage deep-learning model.

Theranostics 2022 18;12(12):5564-5573. Epub 2022 Jul 18.

Department of Radiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Although non-contrast computed tomography (NCCT) is the recommended examination for the suspected acute ischemic stroke (AIS), it cannot detect significant changes in the early infarction. We aimed to develop a deep-learning model to identify early invisible AIS in NCCT and evaluate its diagnostic performance and capacity for assisting radiologists in decision making. : In this multi-center, multi-manufacturer retrospective study, 1136 patients with suspected AIS but invisible lesions in NCCT were collected from two geographically distant institutions between May 2012 to May 2021. The AIS lesions were confirmed based on the follow-up diffusion-weighted imaging and clinical diagnosis. The deep-learning model was comprised of two deep convolutional neural networks to locate and classify. The performance of the model and radiologists was evaluated by the area under the receiver operator characteristic curve (AUC), sensitivity, specificity, and accuracy values with 95% confidence intervals. Delong's test was used to compare the AUC values, and a chi-squared test was used to evaluate the rate differences. 986 patients (728 AIS, median age, 55 years, interquartile range [IQR]: 47-65 years; 664 males) were assigned to the training and internal validation cohorts. 150 patients (74 AIS, median age, 63 years, IQR: 53-75 years; 100 males) were included as an external validation cohort. The AUCs of the model were 83.61% (sensitivity, 68.99%; specificity, 98.22%; and accuracy, 89.87%) and 76.32% (sensitivity, 62.99%; specificity, 89.65%; and accuracy, 88.61%) for the internal and external validation cohorts based on the slices. The AUC of the model was much higher than that of two experienced radiologists (65.52% and 59.48% in the internal validation cohort; 64.01% and 64.39% in external validation cohort; all < 0.001). The accuracy of two radiologists increased from 62.00% and 58.67% to 92.00% and 84.67% when assisted by the model for patients in the external validation cohort. : This deep-learning model represents a breakthrough in solving the challenge that early invisible AIS lesions cannot be detected by NCCT. The model we developed in this study can screen early AIS and save more time. The radiologists assisted with the model can provide more effective guidance in making patients' treatment plan in clinic.
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http://dx.doi.org/10.7150/thno.74125DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330528PMC
August 2022

A Cross-Sectional, Multicenter Study Examining the Validation and Adaptation of the Chinese ROWAN Foot Pain Assessment Questionnaire.

Pain Physician 2022 08;25(5):401-408

Department of Orthopedics, the First Medical Center, Chinese PLA General Hospital, Beijing, China.

Background: The 39-item ROwan Foot Pain Assessment Questionnaire (ROFPAQ) has affective, cognitive, and sensory dimensions to evaluate chronic foot pain. However, to date, the ROFPAQ has only been validated in English and Spanish versions. A simplified Chinese version of ROFPAQ is still not available, even though China has a large population of patients with foot pain.

Objective: This study's aim was to translate the ROFPAQ into a Chinese version and assess its reliability and validity in Chinese patients with chronic foot pain.

Study Design: A cross-sectional, multicenter descriptive study.

Setting: This study took place at the Chinese PLA General Hospital, PLA Strategic Support Force Characteristic Medical Center and Wenzhou integrated traditional Chinese and Western Medicine Hospital Affiliated to Zhejiang traditional Chinese Medicine University.

Methods: The ROFPAQ-C (Chinese) was developed by a forward/backward translation protocol and cross-cultural adaptation from the United Kingdom to China, and from English to Chinese Putonghua. A total of 194 patients from 3 centers with chronic foot pain were recruited for test-retest measures from July 2020 though September 2021.

Results: Adequate internal consistencies (Cronbach's Alpha) in 3 domains ranged from 0.875 to 0.799 for the cognitive, from 0.795 to 0.629 for the affective, and from 0.801 to 0.811 for the sensory, as well as for the total score from 0.880 to 0.815. Adequate test-retest reliability by intraclass correlation coefficient (ICC) were shown in the cognitive 0.712 (95% CI 0.636 to 0.775), the affective 0.929 (95% CI 0.906 to 0.946), the sensory 0.753 (95% CI 0.685 to 0.808), and the total score 0.932 (95% CI 0.910 to 0.948). Adequate item-total correlations were shown for the cognitive from 0.848 to 0.825, the affective from 0.918 to 0.908, and the sensory from 0.943 to 0.855.

Limitations: The original ROFPAQ with 39 items was developed from a podiatry department of the health care national service of the United Kingdom.

Conclusions: The ROFPAQ-C can be used as a valid and reliable tool for chronic foot pain in the Chinese population.
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August 2022

Research on the Identification Model of Medical Damage.

Fa Yi Xue Za Zhi 2022 Apr;38(2):158-165

West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu 610041, China.

Objectives: To understand the perceptions of doctors, patients and forensic examiners on the current situation of medical disputes and medical damage identification in China, and to explore the medical damage identification model that is more conducive for the resolution of medical disputes.

Methods: A questionnaire was designed, and in-service clinicians, forensic examiners and inpatients in Sichuan Province and Chongqing City were randomly selected from April to November 2019. SPSS 22.0 software was used to analyze the data of various survey results.

Results: Compared with patients (24.92%), doctors (61.72%) believed that the current doctor-patient relationship was more tense than before; both doctors and patients were more inclined to choose voluntary consultation and people's mediation to resolve medical disputes; forensic examiners have the highest level of cognition of medical and health-related laws and regulations, followed by doctors and patients; 66.72% of doctors and 78.41% of patients believed that medical damage identification was necessary, and they were more inclined to entrust forensic identification institutions; different groups all believed that forensic examiners and doctors should participate in the identification together, 80.94% of doctors believed that the appraisal institutions should be responsible for the forensic opinion, not the appraiser.

Conclusions: It is suggested that the Medical Association identification and forensic identification should learn from each other and formulate basic unified rules for the identification of medical damage. It is suggested to standardize the behavior of medical damage forensic identification institutions and appraisers, to improve their own appraisal level, actively invite clinical medical experts for consultation in identification, and promote the standardized, scientization of forensic identification.
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http://dx.doi.org/10.12116/j.issn.1004-5619.2022.220105DOI Listing
April 2022

Comparison of Medical Dispute Resolution Mechanisms in China and Abroad.

Fa Yi Xue Za Zhi 2022 Apr;38(2):150-157

School of Forensic Medicine, Shanxi Medical University, Taiyuan 030000, China.

Medical disputes are one of the common problems concerned by the whole world. All countries and regions have established their own medical dispute resolution mechanisms, in accordance with their own national conditions. Medical dispute identification opinions, as one of the important bases for identifying the responsibilities of both doctors and patients, play a pivotal role in the process of dispute settlement. A reasonable medical dispute resolution mechanism and standardized medical dispute identification model can help resolve disputes flexibly and reduce the conflict between doctors and patients. This paper briefly compares the medical dispute resolution mechanism and identification mode of China and several other representative countries (the United States, Britain, France, Germany, Italy, Japan, etc.), and discusses their respective characteristics and shortcomings, to bring some enlightenment to the medical dispute resolution and identification in our country.
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http://dx.doi.org/10.12116/j.issn.1004-5619.2022.220106DOI Listing
April 2022

Comment on: "Thoracic Paravertebral Block Ameliorates POD in Geriatric Patients".

Thorac Cardiovasc Surg 2022 Aug 27;70(5):445-446. Epub 2022 Jul 27.

Department of Anesthesiology, Beijing Friendship Hospital, Capital Medical University, Beijing, People's Republic of China.

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http://dx.doi.org/10.1055/s-0042-1749592DOI Listing
August 2022

Identification of heptapeptides targeting a lethal bacterial strain in septic mice through an integrative approach.

Signal Transduct Target Ther 2022 07 25;7(1):245. Epub 2022 Jul 25.

Guangdong Provincial Key Laboratory of Proteomics, State Key Laboratory of Organ Failure Research, Department of Pathophysiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, China.

Effectively killing pathogenic bacteria is key for the treatment of sepsis. Although various anti-infective drugs have been used for the treatment of sepsis, the therapeutic effect is largely limited by the lack of a specific bacterium-targeting delivery system. This study aimed to develop antibacterial peptides that specifically target pathogenic bacteria for the treatment of sepsis. The lethal bacterial strain Escherichia coli MSI001 was isolated from mice of a cecal ligation and puncture (CLP) model and was used as a target to screen bacterial binding heptapeptides through an integrative bioinformatics approach based on phage display technology and high-throughput sequencing (HTS). Heptapeptides binding to E. coli MSI001 with high affinity were acquired after normalization by the heptapeptide frequency of the library. A representative heptapeptide VTKLGSL (VTK) was selected for fusion with the antibacterial peptide LL-37 to construct the specific-targeting antibacterial peptide VTK-LL37. We found that, in comparison with LL37, VTK-LL37 showed prominent bacteriostatic activity and an inhibitive effect on biofilm formation in vitro. In vivo experiments demonstrated that VTK-LL37 significantly inhibited bacterial growth, reduced HMGB1 expression, alleviated lesions of vital organs and improved the survival of mice subjected to CLP modeling. Furthermore, membrane DEGP and DEGQ were identified as VTK-binding proteins by proteomic methods. This study provides a novel strategy for targeted pathogen killing, which is helpful for the treatment of sepsis in the era of precise medicine.
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http://dx.doi.org/10.1038/s41392-022-01035-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309159PMC
July 2022

Effect of Self-Efficacy Intervention Combined with Humanistic Nursing on Self-Care Ability and Quality of Life in Patients Receiving Chemotherapy for Malignant Tumors.

Iran J Public Health 2022 Feb;51(2):345-354

Oncology Department of Fuyang People's Hospital, Fuyang 341202, Anhui Province, China.

Background: We aimed to explore the effect of self-efficacy intervention combined with humanistic nursing on self-care ability and quality of life in patients receiving chemotherapy for malignant tumors.

Methods: A total of 410 patients were enrolled, who received chemotherapy for malignant tumors in Fuyang People's Hospital from June 2019 to June 2021. They were equally divided into the experimental group and the control group by a random number table. The former was given routine nursing, while self-efficacy intervention combined with humanistic nursing on the bases of routine care was introduced for the latter. Baseline information was collected from all patients. The psychological status of patients before and after intervention was assessed by self-rating anxiety scale (SAS), self-rating depression scale (SDS) and Visual analogue scale (VAS), while self-efficacy score and self-care ability scale for evaluating self-care ability of patients. Additionally, there was an evaluation of quality of life and nursing satisfaction in each group.

Results: Before intervention, no significant difference was identified in psychological status, self-care ability and quality of life between the two groups. After the intervention, the above three indexes in the experimental group were significantly better than those of the control group were. The experimental group had higher nursing satisfaction than the control group.

Conclusion: In patients with malignant tumor undergoing chemotherapy, self-efficacy intervention combined with humanistic nursing can significantly improve the self-care ability, quality of life and nursing satisfaction of patients, which is therefore worthy of promotion in clinical.
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http://dx.doi.org/10.18502/ijph.v51i2.8687DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9273493PMC
February 2022

Integrative Genomic and Transcriptomic Analysis of Primary Malignant Gliomas Revealed Different Patterns Between Grades and Somatic Mutations Related to Glioblastoma Prognosis.

Front Mol Biosci 2022 5;9:873042. Epub 2022 Jul 5.

Department of Pathology, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

As reflected in the WHO classification of glioma since 2020, genomic information has been an important criterion in addition to histology for glioma classification. There is a significant intergrade difference as well as intragrade difference of survival probability among glioma patients. Except the molecular criteria used in the WHO classification, few studies have explored other genomic factors that may be underlying these survival differences, especially in Chinese populations. Here, we used integrative genomic approaches to characterize a Chinese glioma cohort to search for potential prognostic biomarkers. We recruited 46 Chinese patients with primary malignant glioma. All the patients were analyzed with whole-exome sequencing (WES) and 27 of them were analyzed with RNA-seq. We compared the molecular features between patients in different WHO grades. We classified the glioblastoma (GBM) patients into two groups (good vs poor survival) using six-month progression-free survival (PFS6) status and compared the genomic profiles between the two groups. We found grade II and grade III patients cluster together (LGG) and they are different from GBM in unsupervised clustering analysis with RNA-seq data. Gene set enrichment analysis (GSEA) comparing GBM and the LGG group suggested that GBM had upregulation of multiple pathways related to genome integrity and immune cell infiltration. Further comparison of somatic mutations between the two groups revealed as a novel mutation associated with GBM and prevalence of CNV in multiple genes in GBM. Comparison between PFS6 good and poor GBM patients revealed six genes () were significantly mutated and two genes ( and ) had more CNV alterations in the poor prognosis group. Taken together, our molecular data revealed that GBM patient showed distinct characteristics related to individual gene, chromosome integrity, and infiltrating immune cells compared to LGG (grade II/III) patients. We also identified few novel genes with SNV or CNV, which might be the potential markers for clinical outcome of GBM.
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http://dx.doi.org/10.3389/fmolb.2022.873042DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294235PMC
July 2022

Re: Intermittent peri-tubal instillation of dexamethasone and ropivacaine on postoperative analgesia after percutaneous nephrolithotomy: A prospective randomized controlled trial.

Int J Urol 2022 Jul 18. Epub 2022 Jul 18.

Department of Anesthesiology, Beijing Friendship Hospital, Capital Medical University, Beijing, People's Republic of China.

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http://dx.doi.org/10.1111/iju.14975DOI Listing
July 2022

Determination of 5-Formyluracil via Oxime-Based Nucleotide-Metal Coordination.

Chembiochem 2022 Jul 18:e202200355. Epub 2022 Jul 18.

College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, The Institute for Advanced Studies, Hubei Province Key Laboratory of Allergy and Immunology, Wuhan University, Wuhan, 430072, China.

2-(Aminooxy)-N-(quinolin-8-yl)acetamide was synthesized, and its ability to regulate activities of DNA polymerase was tested. In addition, we used the isothermal amplification technology to detect the content of 5-formyluracil sites in irradiated genomic DNA, which confirmed its capability for the detection of 5-formyluracil content in general samples. This study presents the first example of the determination of 5fU based on coordination chemistry.
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http://dx.doi.org/10.1002/cbic.202200355DOI Listing
July 2022

Immune checkpoint inhibitor (ICI)-based treatment beyond progression with prior immunotherapy in patients with stage IV non-small cell lung cancer: a retrospective study.

Transl Lung Cancer Res 2022 Jun;11(6):1027-1037

Department of Thoracic Oncology, Cancer Center, West China Hospital, Sichuan University, Chengdu, China.

Background: Although immune checkpoint inhibitors (ICIs) provide unprecedented survival improvement for patients with advanced non-small cell lung cancer (NSCLC), disease progression inevitably occurs. After ICIs failure, limited data exist on whether ICI-based treatment beyond progression (TBP) may be beneficial to advanced NSCLC. This retrospective study aimed to evaluate the efficacy of this treatment approach in advanced NSCLC and identify potential beneficial factors.

Methods: Patients with stage IV NSCLC who received ICI-based treatment after the failure of prior PD-1/PD-L1 inhibitor treatments (monotherapy or combination therapy) between January 2016 and July 2020 were enrolled. Their clinical characteristics and treatment procedures were collected, and the follow-up would be performed.

Results: A total of 204 patients were included. All patients had disease progression after prior immunotherapy, with 49.5% (101/204) of patients presenting with new metastasis lesions and the rest 50.5% (103/204) of patients' progression on originate lesions. Within the entire cohort, the median progression-free survival (PFS) and median overall survival (OS) of ICI-based TBP with prior immunotherapy were 5.0 months (95% CI: 4.5-5.5 months) and 15.7 months (95% CI: 14.7-16.8 months), respectively. The objective response rate (ORR) and disease control rate (DCR) were 9.3% and 74.0%, respectively. According to the multivariate analysis, ICI-based combination therapy [PFS: hazard ratio (HR), 0.48, 95% confidence interval (CI): 0.28-0.84, P=0.011] (OS: HR, 0.44, 95% CI: 0.23-0.85, P=0.014), not having targetable gene alterations (PFS: HR, 0.56, 95% CI: 0.40-0.79, P=0.001) (OS: HR, 0.57, 95% CI: 0.37-0.87, P=0.009), and good response to prior immunotherapy (PFS: HR, 0.36, 95% CI: 0.24-0.53, P<0.0001) (OS: HR, 0.31, 95% CI: 0.19-0.52, P<0.0001) were independently associated with improved PFS and OS. Moreover, disease progression due to appearances of new metastasis (OS: HR, 0.56, 95% CI: 0.37-0.84, P=0.005) was only associated with better OS.

Conclusions: While the ORR in patients with advanced NSCLC receiving ICI-based TBP with prior immunotherapy was limited, the DCR was relatively high in our study which is encouraging. ICI-based treatment strategy may be a reasonable option for patients who progressed from prior immunotherapy. Further prospective studies on larger sample size are warranted.
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http://dx.doi.org/10.21037/tlcr-22-376DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9271428PMC
June 2022

Hemidiaphragmatic paresis associated with interscalene nerve block.

Can J Anaesth 2022 Jul 13. Epub 2022 Jul 13.

Department of Anesthesiology, Beijing Friendship Hospital, Capital Medical University, Beijing, People's Republic of China.

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http://dx.doi.org/10.1007/s12630-022-02289-yDOI Listing
July 2022
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