Publications by authors named "Tian Li"

1,462 Publications

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A Thermally and Chemically Stable Copper(II) Metal-Organic Framework with High Performance for Gas Adsorption and Separation.

Inorg Chem 2021 Apr 16. Epub 2021 Apr 16.

Tianjin Key Laboratory of Structure and Performance for Functional Molecules, College of Chemistry, Tianjin Normal University, Tianjin 300387, P. R. China.

A versatile microporous metal-organic framework (MOF), {[Cu(TIA)]·1.5CHOH} (), was successfully obtained via the solvothermal reaction of cuprous(II) salt with the bifunctional ligand 3-(1-1,2,4-triazol-1-yl)isophthalic acid. Single-crystal X-ray diffraction studies indicate that contains an three-dimensional skeleton and two types of one-dimensional channels. The framework of has excellent acid-alkali resistance and thermal stability, which is stable in a pH = 2-13 aqueous solution and an 260 °C air environment. In addition, the microporous copper MOF shows very high uptakes of CO (180 cm·g) and CH (113 cm·g) at 273 K and displays excellent adsorption selectivity for small molecular gases. The ideal adsorbed solution theory selectivity values for CH/CH, CO/CH, and CO/N are 2, 9, and 22 at 298 K, respectively. At the same time, breakthrough experiments for CO/CH, CO/N, and CH/CH were further conducted to verify the efficient separation performances.
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http://dx.doi.org/10.1021/acs.inorgchem.1c00357DOI Listing
April 2021

Comprehensive Analysis of the Control of Cancer Stem Cell Characteristics in Endometrial Cancer by Network Analysis.

Comput Math Methods Med 2021 29;2021:6653295. Epub 2021 Mar 29.

Department of Obstetrics and Gynecology, The Seventh Affiliated Hospital, Sun Yat-sen University, China.

Background: Cancer stem cells play an important role in endometrial cancer (EC). It is closely related to self-renewal and therapeutic resistance of EC.

Methods: In this study, WGCNA (weighted gene coexpression network analysis) was used to analyze the relationship between genes and clinical features. We also performed immune cell infiltration analysis of a key module by using ImmuCellAI (Immune Cell Abundance Identifier). Then, key genes were verified in the GEO database. Finally, causal relationship analysis and protein-protein interaction analysis were performed in DisNor tool and STRING.

Result: The mRNA expression-based stemness index (mRNAsi) is significantly lower in normal tissues and is significantly higher in individuals with stage IV or high-grade cancer and those who are obese or postmenopausal. Nineteen key genes (ORC6, C1orf112, RAD54L, SGO2, BUB1, PLK4, KIF18B, BUB1B, TTK, NCAPG, XRCC2, CENPF, KIF15, RACGAP1, ARHGAP11A, TPX2, KIF14, KIF4A, and NCAPH) that were enriched mainly in terms related to the cell cycle and DNA replication were selected by weighted gene coexpression network analysis (WGCNA). Based on the key modules, the numbers of NKT cells, NK cells, and neutrophils in the normal group were significantly higher than those in the cancer group. PLK1, CDK1, and MAD2L1, which were correlated with upstream genes, may be an regulated upstream of key genes.

Conclusion: PLK1, CDK1, and MAD2L1 which were strongly correlated with upstream genes may be a regulated upstream of key genes.
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http://dx.doi.org/10.1155/2021/6653295DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8025127PMC
March 2021

Effects of environmental contaminants in water resources on nonalcoholic fatty liver disease.

Environ Int 2021 Apr 12;154:106555. Epub 2021 Apr 12.

Hunan Provincial Key Laboratory of Clinical Epidemiology, Xiangya School of Public Health, Central South University, Changsha 410078, China; Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, University of South China, Hengyang 421001, China; Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health Southeast University, Nanjing 210009, China. Electronic address:

The global prevalence of nonalcoholic fatty liver disease (NAFLD) has been increasing rapidly in recent years, which is now estimated to be over 25%. NAFLD is one of the most common chronic liver diseases in the world. At present, with the rapid development of economy and industrialization, many chemicals are released into the environment. These chemical contaminants in the environment might cause harm to human health and result in lipid metabolism disorder during long-term exposure. Moreover, the incentive of many NAFLD cases is unknown, and the environmental risk factors of NAFLD need to be urgently identified. Hence, we focus on the impacts of several popular environmental contaminants in water environment on the development and progression of NAFLD. These contaminants mainly include microcystins (MCs), disinfection by-products (DBPs), heavy metals (HMs), dioxins and polychlorinated biphenyls (PCBs). Through analyzing a great many epidemiological and toxicological studies, we have found positive associations between NAFLD and chronic exposure to these contaminants at the environmental levels. This review may enhance the understanding of liver damage caused by environmental pollutants, which are considered as tangible environmental risk factors for NAFLD.
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http://dx.doi.org/10.1016/j.envint.2021.106555DOI Listing
April 2021

SIRT6 in Senescence and Aging-Related Cardiovascular Diseases.

Front Cell Dev Biol 2021 29;9:641315. Epub 2021 Mar 29.

Department of Cardiology, China Tangdu Hospital, Fourth Military Medical University, Xi'an, China.

SIRT6 belongs to the nicotinamide adenine dinucleotide (NAD)-dependent deacetylases and has established diverse roles in aging, metabolism and disease. Its function is similar to the , which prolongs lifespan and regulates genomic stability, telomere integrity, transcription, and DNA repair. It has been demonstrated that increasing the sirtuin level through genetic manipulation extends the lifespan of yeast, nematodes and flies. Deficiency of SIRT6 induces chronic inflammation, autophagy disorder and telomere instability. Also, these cellular processes can lead to the occurrence and progression of cardiovascular diseases (CVDs), such as atherosclerosis, hypertrophic cardiomyopathy and heart failure. Herein, we discuss the implications of SIRT6 regulates multiple cellular processes in cell senescence and aging-related CVDs, and we summarize clinical application of SIRT6 agonists and possible therapeutic interventions in aging-related CVDs.
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http://dx.doi.org/10.3389/fcell.2021.641315DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039379PMC
March 2021

Correction to: A case report of Phelan-McDermid syndrome: preliminary results of the treatment with growth hormone therapy.

Ital J Pediatr 2021 Apr 14;47(1):89. Epub 2021 Apr 14.

Affiliated Hospital of Jiangnan University, No. 1000, Hefeng Avenue, Wuxi, 214122, People's Republic of China.

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http://dx.doi.org/10.1186/s13052-021-01038-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8045318PMC
April 2021

Transcriptomic analysis identifies upregulation of secreted phosphoprotein 1 in silicotic rats.

Exp Ther Med 2021 Jun 31;21(6):579. Epub 2021 Mar 31.

School of Public Health, Hebei Key Laboratory for Organ Fibrosis Research, North China University of Science and Technology, Tangshan, Hebei 063210, P.R. China.

Silicosis is caused by exposure to crystalline silica and the molecular mechanism of silicotic fibrosis remains unclear. Therefore, the present study investigated the mRNA profiles of rats exposed to crystalline silica. RNA-sequencing techniques were used to observe differential expression of mRNAs in silicotic rats induced by chronic inhalation of crystalline silica particulates. Prediction of mRNA functions and signaling pathways was conducted using Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. Certain differentially expressed mRNAs were verified in lung tissue of silicotic rats by quantitative polymerase chain reaction (qPCR). Secreted phosphoprotein 1 (SPP1) was measured in serum from silicosis patients, lungs of silicotic rats and NR8383 macrophages treated with silica. A total of 1,338 mRNAs were revealed to be differentially expressed in silicotic rat lungs, including 912 upregulated and 426 downregulated mRNAs. In GO analysis of significant changes in mRNAs, the most affected processes were the defense response, extracellular space and chemokine activity in terms of biological process, cellular component and molecular function. In KEGG pathway analysis, dysregulated mRNAs were involved in systemic lupus erythematosus, staphylococcus aureus infection, complement and coagulation cascades, alcoholism and pertussis. qPCR demonstrated that expression of , , , , and was increased in silicotic rats, while , , and expression was decreased. It was also found that SPP1 was increased in serum from silicosis patients, silicotic rats and silica-treated NR8383 macrophages. The expression of mRNAs was altered significantly in silicotic rats, which suggested that certain genes are novel targets for the diagnosis and treatment of silicosis.
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http://dx.doi.org/10.3892/etm.2021.10011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8027763PMC
June 2021

TIGIT-Fc as a Potential Therapeutic Agent for Fetomaternal Tolerance.

Front Immunol 2021 25;12:649135. Epub 2021 Mar 25.

Department of Biophysics, College of Basic Medical Sciences, Second Military Medical University, Shanghai, China.

The perfect synchronization of maternal immune-endocrine mechanisms and those of the fetus is necessary for a successful pregnancy. In this report, decidual immune cells at the maternal-fetal interface were detected that expressed TIGIT (T cell immunoreceptor with Ig and ITIM domains), which is a co-inhibitory receptor that triggers immunological tolerance. We generated recombinant TIGIT-Fc fusion proteins by linking the extracellular domain of TIGIT and silent Fc fragments. The treatment with TIGIT-Fc of human decidual antigen presenting cells (APCs), the decidual dendritic cells (dDCs), and decidual macrophages (dMϕs) increased the production of interleukin 10 and induced the decidua APCs to powerfully polarize the decidual CD4 T cells toward a classic T2 phenotype. We further proposed that Notch signaling shows a pivotal effect on the transcriptional regulation in decidual immune cell subsets. Moreover, the administration of TIGIT-Fc to CBA/J pregnant mice at preimplantation induced CD4 forkhead box P3 (Foxp3) regulatory T cells and tolerogenic dendritic cells and increased pregnancy rates in an abortion-prone animal model stress. The results suggested the therapeutic potential of the TIGIT-Fc fusion protein in reinstating immune tolerance in failing pregnancies.
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http://dx.doi.org/10.3389/fimmu.2021.649135DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8027249PMC
March 2021

Differences in Radiomics Signatures Between Patients with Early and Advanced T-Stage Nasopharyngeal Carcinoma Facilitate Prognostication.

J Magn Reson Imaging 2021 Apr 8. Epub 2021 Apr 8.

School of Physics, State Key Laboratory of Optoelectronic Materials and Technologies, Sun Yat-sen University, Guangzhou, PR China.

Background: Accurately predicting the risk of death, recurrence, and metastasis of patients with nasopharyngeal carcinoma (NPC) is potentially important for personalized diagnosis and treatment. Survival outcomes of patients vary greatly in distinct stages of NPC. Prognostic models of stratified patients may aid in prognostication.

Purpose: To explore the prognostic performance of MRI-based radiomics signatures in stratified patients with NPC.

Study Type: Retrospective.

Population: Seven hundred and seventy-eight patients with NPC (T1-2 stage: 298, T3-4 stage: 480; training cohort: 525, validation cohort: 253).

Field Strength/sequence: Fast-spin echo (FSE) axial T1-weighted images, FSE axial T2-weighted images, contrast-enhanced FSE axial T1-weighted images at 1.5 T or 3.0 T.

Assessment: Radiomics signatures, clinical nomograms, and radiomics nomograms combining the radiomic score (Radscore) and clinical factors for predicting progression-free survival (PFS) were constructed on T1-2 stage patient cohort (A), T3-4 stage patient cohort (B), and the entire dataset (C).

Statistical Tests: Least absolute shrinkage and selection operator (LASSO) method was applied for radiomics modeling. Harrell's concordance indices (C-index) were employed to evaluate the predictive power of each model.

Results: Among 4,410 MRI-extracted features, we selected 16, 16, and 14 radiomics features most relevant to PFS for Models A, B, and C, respectively. Only 0, 1, and 4 features were found overlapped between models A/B, A/C, and B/C, respectively. Radiomics signatures constructed on T1-2 stage and T3-4 stage patients yielded C-indices of 0.820 (95% confidence interval [CI]: 0.763-0.877) and 0.726 (0.687-0.765), respectively, which were larger than those on the entire validation cohort (0.675 [0.637-0.713]). Radiomics nomograms combining Radscore and clinical factors achieved significantly better performance than clinical nomograms (P < 0.05 for all).

Data Conclusion: The selected radiomics features and prognostic performance of radiomics signatures differed per the type of NPC patients incorporated into the models. Radiomics models based on pre-stratified tumor stages had better prognostic performance than those on unstratified dataset.

Level Of Evidence: 4 Technical Efficacy Stage: 5.
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http://dx.doi.org/10.1002/jmri.27633DOI Listing
April 2021

Autologous decellularized extracellular matrix promotes adipogenic differentiation of adipose derived stem cells in low serum culture system by regulating the ERK1/2-PPARγ pathway.

Adipocyte 2021 12;10(1):174-188

Deprtment of Plastic Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou City, China.

High viability and further adipogenic differentiation of adipose-derived stem cells (ADSCs) are fundamental for engraftment and growth of the transplanted adipose tissue. It has been demonstrated that extracellular matrix (ECM) regulates cell proliferation and differentiation by interacting with ERK1/2 signalling pathway. In this study, we prepared autologous decellularized extracellular matrix (d-ECM) and explored its effect on the proliferation and adipogenic ability of ADSCs in low serum culture. We found that 2% foetal bovine serum (FBS) in growth medium inhibited cell viability and DNA replication, and decreased mRNA and protein levels of PPARγ and C/EPBα compared with 10% FBS. Correspondingly, after 14-days adipogenic induction, cells cultured in 2% FBS possessed lower efficiency of adipogenesis and expressed less adipocyte differentiation markers ADIPOQ and aP2. On the contrary, the d-ECM-coated substrate continuously promoted the expression of PPARγ, and regulated the phosphorylation of ERK1/2 in different manners during differentiation. Pretreatment with ERK1/2 inhibitor PD98059 neutralized the effects of d-ECM, which suggested d-ECM might regulate the adipogenesis of ADSCs through ERK1/2-PPARγ pathway. In addition, d-ECM was revealed to regulate the transcription and expression of stemness-associated genes, such as OCT4, NANOG and SOX2, in the undifferentiated ADSCs, which might be related to the initiation of differentiation.
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http://dx.doi.org/10.1080/21623945.2021.1906509DOI Listing
December 2021

Investigation of the effect of acquisition schemes on time-resolved magnetic resonance fingerprinting.

Phys Med Biol 2021 Apr 6. Epub 2021 Apr 6.

Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hongkong, CHINA.

Purpose: This study aims to investigate the feasibility of different acquisition methods for time-resolved magnetic resonance fingerprinting (TR-MRF) in computer simulation.

Methods: Extended cardiac-torso (XCAT) phantom is used to generate abdominal T1, T2, and proton density (PD) maps for MRF simulation. The simulated MRF technique consists of an IR-FISP MRF sequence with spiral trajectory acquisition. MRF maps were simulated with different number of repetitions from 1 to 15. Three different methods were used to generate TR-MRF maps: 1) continuous acquisition without delay between MRF repetitions; 2) continuous acquisition with 5 seconds delay between MRF repetitions; 3) triggered acquisition with variable delay between MRF repetitions to allow the next acquisition to start at different respiration phase. After the generation of TR-MRF maps, the image quality indexes including absolute T1 and T2 value, signal-to-noise-ratio (SNR), tumor-to-liver contrast-to-noise ratio (CNR), error in the amplitude of diaphragm motion and tumor volume error were used to evaluate the reconstructed parameter maps. Three volunteers were recruited to test the feasibility of the selected acquisition method.

Results: Dynamic MR parametric maps using three different acquisition methods were estimated. The overall and liver T1 value error, liver SNR in T1 and T2 maps, and tumor SNR from T1 maps from triggered method is statistically significantly better than the other two methods (p-value < 0.05). The other image quality indexes have no significant difference between the triggered method and the other two continuous acquisition methods. All image quality indexes exhibit no significant difference between the acquisition methods with 0 second and 5 seconds delay. The triggered method was successfully performed in three healthy volunteers.

Conclusion: TR-MRF technique was investigated using three different acquisition methods in computer simulation where the triggered method showed better performance than the other two methods. The triggered method has been tested successfully in healthy volunteers.
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http://dx.doi.org/10.1088/1361-6560/abf51fDOI Listing
April 2021

Zipcode RNA-Binding Proteins and Membrane Trafficking Proteins Cooperate to Transport Glutelin mRNAs in Rice Endosperm.

Plant Cell 2020 Aug;32(8):2566-2581

Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340.

In rice (Oryza sativa) endosperm cells, mRNAs encoding glutelin and prolamine are translated on distinct cortical-endoplasmic reticulum (ER) subdomains (the cisternal-ER and protein body-ER), a process that facilitates targeting of their proteins to different endomembrane compartments. Although the cis- and trans-factors responsible for mRNA localization have been defined over the years, how these mRNAs are transported to the cortical ER has yet to be resolved. Here, we show that the two interacting glutelin zipcode RNA binding proteins (RBPs), RBP-P and RBP-L, form a quaternary complex with the membrane fusion factors n-ethylmaleimide-sensitive factor (NSF) and the small GTPase Rab5a, enabling mRNA transport on endosomes. Direct interaction of RBP-L with Rab5a, between NSF and RBP-P, and between NSF and Rab5a, were established. Biochemical and microscopic analyses confirmed the co-localization of these RBPs with NSF on Rab5a-positive endosomes that carry glutelin mRNAs. Analysis of a loss-of-function rab5a mutant showed that glutelin mRNA and the quaternary complex were mis-targeted to the extracellular paramural body structure formed by aborted endosomal trafficking, further confirming the involvement of endosomal trafficking in glutelin mRNA transport. Overall, these findings demonstrate that mRNA localization in plants co-opts membrane trafficking via the acquisition of new functional binding properties between RBPs and two essential membrane trafficking factors, thus defining an endosomal anchoring mechanism in mRNA localization.
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http://dx.doi.org/10.1105/tpc.20.00111DOI Listing
August 2020

A fungal sirtuin modulates development and virulence in the insect pathogen, Beauveria bassiana.

Environ Microbiol 2021 Apr 5. Epub 2021 Apr 5.

Department of Microbiology and Cell Science, University of Florida, Bldg. 981, Museum Rd, Gainesville, FL, 32611, USA.

Chromatin transitions are mediated in part by acetylation/deacetylation post-translational modifications of histones. Histone deacetylases, e.g. sirtuins (Sir-proteins), repress transcription via promotion of heterochromatin formation. Here, we characterize the Sir2 class III histone deacetylase (BbSir2) in the environmentally and economically important fungal insect pathogen, Beauveria bassiana. BbSir2 is shown to contribute to the deacetylation of lysine residues on H3 and H4 histones. Targeted gene knockout of BbSir2 resulted in impaired asexual development, reduced abilities to utilize various carbon/nitrogen sources, reduced tolerance to oxidative, heat, and UV stress, and attenuated virulence. ΔBbSir2 cells showed disrupted cell cycle development and abnormal hyphal septation patterns. Proteomic protein acetylation analyses of wild type and ΔBbSir2 cells, revealed the differential abundance of 462 proteins, and altered (hyper- or hypo-) acetylation of 436 lysine residues on 350 proteins. Bioinformatic analyses revealed enrichment in pathways involved in carbon/nitrogen metabolism, cell cycle control and cell rescue, defense, and mitochondrial functioning. Critical targets involved in virulence included LysM effector proteins and a benzoquinone oxidoreductase implicated in detoxification of cuticular compounds. These data indicate broad effects of BbSir2 on fungal development and stress response, with identification of discrete targets that can account for the observed (decreased) virulence phenotype. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1111/1462-2920.15497DOI Listing
April 2021

, the Predominant Bacterium to Predict the Severity of Liver Injury in Alcoholic Liver Disease.

Front Cell Infect Microbiol 2021 17;11:649060. Epub 2021 Mar 17.

Geriatrics Digestion Department of Internal Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.

Background: New evidence implies that the imbalance of gut microbiota is associated with the progression of alcoholic liver disease (ALD) and that the composition of gut microbiota is altered in ALD patients. However, the predominant bacterium in patients involved in the progress of ALD has not been identified. The purpose of this study is to investigate the predominant bacterium in the early and end-stages of ALD as well as the relationship between the bacterium and the degree of liver injury.

Methods: We enrolled 21 alcoholic fatty liver (AFL) patients, 17 alcoholic liver cirrhosis (ALC) patients and 27 healthy controls, and sequenced the 16S rRNA gene of their fecal microbiota. The gut microbiota composition and its relationship with the indicators of clinical hepatic function were assessed using canonical correspondence analysis (CCA), spearman correlation heatmap and multivariate association with linear (MaAsLin) Models.

Results: The composition and structure of gut microbiota changed greatly in different stages of ALD, and the degree of disorder was aggravated with the progression of ALD, even in the early stage. Moreover, the relative abundance of was highly enriched only in patients with ALC (P <0.001), and positively correlated with AST level (P = 0.029). The abundance of distinguished the liver injury of ALC patients from the controls with an area under the receiver-operating characteristic curve (AUC) of 0.877 (P < 0.001).

Conclusions: These findings indicate that the imbalance of gut microbiota exists at the early and end-stages of ALD, and the degree of disorder is aggravated with the progression of ALD. , as the predominant bacterium, may be a microbiological marker to evaluate the severity of liver injury in ALD patients.
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http://dx.doi.org/10.3389/fcimb.2021.649060DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8010180PMC
March 2021

Volume Deformation of Steam-Cured Concrete with Slag during and after Steam Curing.

Materials (Basel) 2021 Mar 27;14(7). Epub 2021 Mar 27.

School of Civil Engineering, Qingdao University of Technology, No.11 of Fushun Road, Qingdao 266033, China.

In order to better predict the development of shrinkage deformation of steam-cured concrete mixed with slag, a deformation-temperature-humidity integrated model test, a hydration heat test, and an elastic modulus test were performed. The effects of the steam-curing process and the content of slag on shrinkage deformation, hydration degree and elastic modulus of concrete were studied. The results indicate that during the steam-curing process, the concrete has an "expansion-shrinkage" pattern. After the steam curing, the deformation of concrete is dominated by drying shrinkage. After the addition of slag, the shrinkage deformation of steam-cured concrete is increased. The autogenous shrinkage increases by 0.5-12%, and the total shrinkage increases by 1.5-8% at 60 days. At the same time, slag reduces the hydration degree of steam-cured concrete and modulus of elasticity. A prediction model for the hydration degree of steam-cured concrete is established, which can be used to calculate the degree of hydration at any curing age. Based on the capillary tension generated by the capillary pores in concrete, an integrated model of autogenous shrinkage and total shrinkage is established with the relative humidity directly related to the water loss in the concrete as the driving parameter. Whether the shrinkage deformation is caused by hydration reaction or the external environment, this model can better predict the shrinkage deformation of steam-cured concrete.
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http://dx.doi.org/10.3390/ma14071647DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8037895PMC
March 2021

Complete chloroplast genome of (Theaceae), a shrub with gorgeous flowers and rich seed oil.

Mitochondrial DNA B Resour 2021 Mar 15;6(3):840-841. Epub 2021 Mar 15.

Jiangxi Provincial Key Laboratory of Camellia Germplasm Conservation and Utilization, Jiangxi Academy of Forestry, Nanchang, China.

Hu is an oil-tea species with high economic and nutritional value in the south of China. In this study, the chloroplast genome of was determined by Illumina Miseq platform. The whole chloroplast genome is 156,971 bp in length, containing a large single-copy region (LSC, 86,673 bp), a small single-copy region (SSC, 18,394 bp), and a pair of inverted repeat regions (IRa and IRb, 25,952 bp). There is a total of 113 genes in the complete chloroplast genome of which 19 genes are repeated in the IR regions. In addition, the phylogenetic tree revealed a close relationship between and . The complete chloroplast genome will contribute to further studies on phylogeny and conservation of and related taxa in of Theaceae.
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http://dx.doi.org/10.1080/23802359.2021.1884026DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7971316PMC
March 2021

Effects of neuroactive metabolites of the tryptophan pathway on working memory and cortical thickness in schizophrenia.

Transl Psychiatry 2021 Apr 1;11(1):198. Epub 2021 Apr 1.

Maryland Psychiatric Research Center, Department of Psychiatry, University of Maryland School of Medicine, Baltimore, MD, USA.

A number of tryptophan metabolites known to be neuroactive have been examined for their potential associations with cognitive deficits in schizophrenia. Among these metabolites, kynurenic acid (KYNA), 5-hydroxyindole (5-HI), and quinolinic acid (QUIN) are documented in their diverse effects on α-7 nicotinic acetylcholine receptor (α7nAChR) and/or N-methyl-D-aspartate receptor (NMDAR), two of the receptor types thought to contribute to cognitive impairment in schizophrenia. In this study, serum levels of KYNA, 5-HI, and QUIN were measured in 195 patients with schizophrenia and in 70 healthy controls using liquid chromatography-tandem mass spectrometry; cognitive performance in MATRICS Consensus Cognitive Battery and cortical thickness measured by magnetic resonance imaging were obtained. Patients with schizophrenia had significantly lower serum KYNA (p < 0.001) and QUIN (p = 0.02) levels, and increased 5-HI/KYNA (p < 0.001) and QUIN/KYNA ratios (p < 0.001) compared with healthy controls. Multiple linear regression showed that working memory was positively correlated with serum 5-HI levels (t = 2.10, p = 0.04), but inversely correlated with KYNA concentrations (t = -2.01, p = 0.05) in patients. Patients with high 5-HI and low KYNA had better working memory than other subgroups (p = 0.01). Higher 5-HI levels were associated with thicker left lateral orbitofrontal cortex (t = 3.71, p = 2.94 × 10) in patients. The different effects of 5-HI and KYNA on working memory may appear consistent with their opposite receptor level mechanisms. Our findings appear to provide a new insight into the dynamic roles of tryptophan pathway metabolites on cognition, which may benefit novel therapeutic development that targets cognitive impairment in schizophrenia.
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http://dx.doi.org/10.1038/s41398-021-01311-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8016899PMC
April 2021

The largest meta-analysis on the global prevalence of microsporidia in mammals, avian and water provides insights into the epidemic features of these ubiquitous pathogens.

Parasit Vectors 2021 Apr 1;14(1):186. Epub 2021 Apr 1.

State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400715, China.

Background: Microsporidia are obligate intracellular parasites that can infect nearly all invertebrates and vertebrates, posing a threat to public health and causing large economic losses to animal industries such as those of honeybees, silkworms and shrimp. However, the global epidemiology of these pathogens is far from illuminated.

Methods: Publications on microsporidian infections were obtained from PubMed, Science Direct and Web of Science and filtered according to the Newcastle-Ottawa Quality Assessment Scale. Infection data about pathogens, hosts, geography and sampling dates were manually retrieved from the publications and screened for high quality. Prevalence rates and risk factors for different pathogens and hosts were analyzed by conducting a meta-analysis. The geographic distribution and seasonal prevalence of microsporidian infections were drawn and summarized according to sampling locations and date, respectively.

Results: Altogether, 287 out of 4129 publications up to 31 January 2020 were obtained and met the requirements, from which 385 epidemiological data records were retrieved and effective. The overall prevalence rates in humans, pigs, dogs, cats, cattle, sheep, nonhuman primates and fowl were 10.2% [2429/30,354; 95% confidence interval (CI) 9.2-11.2%], 39.3% (2709/5105; 95% CI 28.5-50.1%), 8.8% (228/2890; 95% CI 5.1-10.1%), 8.1% (112/1226; 95% CI 5.5-10.8%), 16.6% (2216/12,175; 95% CI 13.5-19.8%), 24.9% (1142/5967; 95% CI 18.6-31.1%), 18.5% (1388/7009; 95% CI 13.1-23.8%) and 7.8% (725/9243; 95% CI 6.4-9.2%), respectively. The higher prevalence in pigs suggests that routine detection of microsporidia in animals should be given more attention, considering their potential roles in zoonotic disease. The highest rate was detected in water, 58.5% (869/1351; 95% CI 41.6-75.5%), indicating that water is an important source of infections. Univariate regression analysis showed that CD4+ T cell counts and the living environment are significant risk factors for humans and nonhuman primates, respectively. Geographically, microsporidia have been widely found in 92 countries, among which Northern Europe and South Africa have the highest prevalence. In terms of seasonality, the most prevalent taxa, Enterocytozoon bieneusi and Encephalitozoon, display different prevalence trends, but no significant difference between seasons was observed. In addition to having a high prevalence, microsporidia are extremely divergent because 728 genotypes have been identified in 7 species. Although less investigated, microsporidia coinfections are more common with human immunodeficiency virus and Cryptosporidium than with other pathogens.

Conclusions: This study provides the largest-scale meta-analysis to date on microsporidia prevalence in mammals, birds and water worldwide. The results suggest that microsporidia are highly divergent, widespread and prevalent in some animals and water and should be further investigated to better understand their epidemic features.
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http://dx.doi.org/10.1186/s13071-021-04700-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8017775PMC
April 2021

Expanding the scope of plant genome engineering with Cas12a orthologs and highly multiplexable editing systems.

Nat Commun 2021 03 29;12(1):1944. Epub 2021 Mar 29.

Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, USA.

CRISPR-Cas12a is a promising genome editing system for targeting AT-rich genomic regions. Comprehensive genome engineering requires simultaneous targeting of multiple genes at defined locations. Here, to expand the targeting scope of Cas12a, we screen nine Cas12a orthologs that have not been demonstrated in plants, and identify six, ErCas12a, Lb5Cas12a, BsCas12a, Mb2Cas12a, TsCas12a and MbCas12a, that possess high editing activity in rice. Among them, Mb2Cas12a stands out with high editing efficiency and tolerance to low temperature. An engineered Mb2Cas12a-RVRR variant enables editing with more relaxed PAM requirements in rice, yielding two times higher genome coverage than the wild type SpCas9. To enable large-scale genome engineering, we compare 12 multiplexed Cas12a systems and identify a potent system that exhibits nearly 100% biallelic editing efficiency with the ability to target as many as 16 sites in rice. This is the highest level of multiplex edits in plants to date using Cas12a. Two compact single transcript unit CRISPR-Cas12a interference systems are also developed for multi-gene repression in rice and Arabidopsis. This study greatly expands the targeting scope of Cas12a for crop genome engineering.
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http://dx.doi.org/10.1038/s41467-021-22330-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8007695PMC
March 2021

Dynamic Elimination of Enrofloxacin Under Varying Temperature and pH in Aquaculture Water: An Orthogonal Study.

Bull Environ Contam Toxicol 2021 May 25;106(5):866-872. Epub 2021 Mar 25.

Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, PR China.

Orthogonal experiments were used to simulate the enrofloxacin (ENR) elimination dynamic in deeper water of aquaculture. Two factors at values common in fishery water (temperature of 20°C, 25°C, and 30°C; pH of 5, 7, and 9) were studied. The degradation of ENR in the nine treatment groups ranged from 44.7 to 80.1%. Variance analysis indicated that pH had a strong impact on the elimination of ENR, while temperature changes showed little effect. The ENR removal rate was highest at a combination of 25°C and pH 5. The optimal conditions of eliminating ENR were performed for exploring the generation of ciprofloxacin (CIP), which indicated that higher ENR concentrations led to the production of greater amounts of CIP. The half-time of ENR was increased 2.02-times in the ENR concentrations increasing from 20 to 2000 ng/mL. This study could increase our understanding of the behaviors of ENR and CIP during the aquaculture process.
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http://dx.doi.org/10.1007/s00128-021-03199-3DOI Listing
May 2021

Editorial Expression of Concern: Triptolide inhibits angiogenesis in microvascular endothelial cells through regulation of miR-92a.

J Physiol Biochem 2021 03 25. Epub 2021 Mar 25.

Medical Examination Center of Qilu Hospital of Shandong University, No.107 Culture West Road, Jinan, 250012, Shandong, China.

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http://dx.doi.org/10.1007/s13105-021-00804-1DOI Listing
March 2021

Identification of a Metabolism-Related Signature for the Prediction of Survival in Endometrial Cancer Patients.

Front Oncol 2021 8;11:630905. Epub 2021 Mar 8.

Department of Obstetrics and Gynecology, Peking University People's Hospital, Beijing, China.

Objective: Endometrial cancer (EC) is one of the most common gynecologic malignancies. The present study aims to identify a metabolism-related biosignature for EC and explore the molecular immune-related mechanisms underlying the tumorigenesis of EC.

Methods: Transcriptomics and clinical data of EC were retrieved from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. Common differentially expressed metabolism-related genes were extracted and a risk signature was identified by using the least absolute shrinkage and selection operator (LASSO) regression analysis method. A nomogram integrating the prognostic model and the clinicopathological characteristics was established and validated by a cohort of clinical EC patients. Furthermore, the immune and stromal scores were observed and the infiltration of immune cells in EC cells was analyzed.

Results: Six genes, including CA3, HNMT, PHGDH, CD38, PSAT1, and GPI, were selected for the development of the risk prediction model. The Kaplan-Meier curve indicated that patients in the low-risk group had considerably better overall survival (OS) (P = 7.874e-05). Then a nomogram was constructed and could accurately predict the OS (AUC = 0.827, 0.821, 0.845 at 3-, 5-, and 7-year of OS). External validation with clinical patients showed that patients with low risk scores had a longer OS (p = 0.04). Immune/stromal scores and infiltrating density of six types of immune cells were lower in high-risk group.

Conclusions: In summary, our work provided six potential metabolism-related biomarkers as well as a nomogram for the prognosis of EC patients, and explored the underlying mechanism involved in the progression of EC.
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http://dx.doi.org/10.3389/fonc.2021.630905DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982602PMC
March 2021

Significantly Enhancing the Lithium Ionic Conductivity of Metal-Organic Frameworks via a Postsynthetic Modification Strategy.

Langmuir 2021 Apr 24;37(13):3922-3928. Epub 2021 Mar 24.

College of Chemistry and Chemical Engineering, Inner Mongolia University, Hohhot 010021, P. R. China.

Metal-organic frameworks (MOFs), due to their possessing a porous structure, are potential candidates for solid-state ionic conduction materials. Moreover, uncoordinated carboxylic acid groups (-COOH) of MOFs can be used as postsynthetic modification sites, which are favorable for lithium ion exchange. Herein, we synthesized a unique multiple carboxylic zinc metal-organic framework (Zn-MOF-COOH) containing uncoordinated carboxylic acid groups. Zn-MOF-COOLi was synthesized through deprotonation using LiOH via a straightforward acid-base reaction at room temperature (RT), thereby exhibiting better good electrochemical properties. The lithium ionic conductivity (σ) increased from 1.81 × 10 to 1.65 × 10 S·cm, lithium ion transference number () rose from 0.67 to 0.77, and the electrochemical window improved from 2.0-5.5 to 1.5-6.5 V. This work offers a new strategy to improve the σ of MOFs and a new perspective toward manufacturing of high-performance solid-state ionic conduction materials.
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http://dx.doi.org/10.1021/acs.langmuir.1c00156DOI Listing
April 2021

Natural abundance of C and N provides evidence for plant-soil carbon and nitrogen dynamics in a N-fertilized meadow.

Ecology 2021 Mar 23:e03348. Epub 2021 Mar 23.

Erguna Forest-Steppe Ecotone Ecosystem Research Station, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110016, China.

Natural abundance of carbon (C) and nitrogen (N) stable isotope ratios (δ C and δ N) has been used to indicate ecosystem C and N status and cycling; however, use of this approach to infer plant and microbial N preference under projected ecosystem N enrichment is limited. Here, we investigated natural abundance δ C and δ N of five dominant plant species, and soil δ N of microbial biomass and available N forms under N addition in a meadow steppe. Additional N, applied as urea, led to decreases in δ N of soil NO (δ N , from 3.0 to 0.4‰) and increases in δ N of soil NH (δ N , from -1.3 to 11‰) and dissolved organic N (δ N , from 8.5 to 15‰) that reflected increased net nitrification rates, a possible increase in NH volatilization, and greater availability of the three N forms. An overall increase in δ N of soil total N (δ N ) from 7.1 to 7.9‰ indicated accelerated and greater openness of soil N cycling that was also partially revealed by enhanced net N mineralization rates. Plant δ N, which ranged from -1.8 to 2.1‰, generally decreased with N addition, indicating a greater reliance on soil NO under N-enrichment conditions. Nitrogen addition decreased δ N of microbial biomass N (from 14 to 2.8‰), possibly due to a shift in preferential N form (DON to NO ), that indicated a convergence of plant and microbial preferential N forms and an increase in plant-microbial N competition. Microbes were thus more flexible than plants in the use of different forms of N. Addition of N decreased plant litter δ C, while plant species δ C remained unaffected, likely due to a shift in the abundance of dominant species with a greater proportion of biomass coming from δ C-depleted species. Enrichment factor (the difference in plant δ N relative to δ N ) of four non-legume species was negatively related to soil inorganic N availability, net nitrification rate, and net N mineralization rate, and was proven to be a good indicator of ecosystem N status. Our study highlights the importance of natural abundance of N as an indicator of plant-microbial N competition and ecosystem N cycling in meadow steppe grasslands under projected ecosystem N enrichment.
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http://dx.doi.org/10.1002/ecy.3348DOI Listing
March 2021

Case Report: Identification of a Missense Mutation in the Gene, p.(Phe690Leu)/c.2070C > A, Causing Hemophilia A: A Case Report.

Front Genet 2020 5;11:589899. Epub 2021 Mar 5.

Assisted Reproductive Center, Women's & Children's Hospital of Northwest, Xi'an, China.

Hemophilia A is an X-linked recessive bleeding disorder caused by various types of pathological defects in the factor VIII gene (FVIII). Preimplantation genetic testing for monogenic disease (PGT-M) is a powerful tool to tackle the transmission of monogenic inherited disorders from generation to generation. In our case, a mutation in had passed through female carriers in a hemophilia A family and resulted in two male patients with hemophilia A. To identify the etiological genetic variants of , next-generation sequencing (NGS) was used for chromosome copy number variation detection, Sanger sequencing to verify mutation sites, single nucleotide polymorphism (SNP) for site amplification, and sequencing to validate the genetic linkage. Finally, a novel missense mutation, p. (Phe690Leu)/c.2070C > A, occurring in exon 13 of , was screened out as a pathogenic mutation. Following this, an normal euploid blastocyst was transferred. At the 18th week, the pregnant mother underwent amniocentesis, NGS, Sanger sequencing, and SNP typing that further confirmed that the fetus had a healthy genotype. After delivery, a neonatal blood sample was sent for FVIII concentration detection, and the result established that the FVIII protein was rescued to a nearly average level. We first identified a new type of pathogenic mutation in , which has not been previously reported, selected a genetically healthy progeny for an affected family, and provided valuable knowledge of the diagnosis and treatment of hemophilia A.
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http://dx.doi.org/10.3389/fgene.2020.589899DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7973284PMC
March 2021

The shape of pleomorphic virions determines resistance to cell-entry pressure.

Nat Microbiol 2021 Mar 18. Epub 2021 Mar 18.

Biochemistry Department, Brandeis University, Waltham, MA, USA.

Many enveloped animal viruses produce a variety of particle shapes, ranging from small spherical to long filamentous types. Characterization of how the shape of the virion affects infectivity has been difficult because the shape is only partially genetically encoded, and most pleomorphic virus structures have no selective advantage in vitro. Here, we apply virus fractionation using low-force sedimentation, as well as antibody neutralization coupled with RNAScope, single-particle membrane fusion experiments and stochastic simulations to evaluate the effects of differently shaped influenza A viruses and influenza viruses pseudotyped with Ebola glycoprotein on the infection of cells. Our results reveal that the shape of the virus particles determines the probability of both virus attachment and membrane fusion when viral glycoprotein activity is compromised. The larger contact interface between a cell and a larger particle offers a greater probability that several active glycoproteins are adjacent to each other and can cooperate to induce membrane merger. Particles with a length of tens of micrometres can fuse even when 95% of the glycoproteins are inactivated. We hypothesize that non-genetically encoded variable particle shapes enable pleomorphic viruses to overcome selective pressure and may enable adaptation to infection of cells by emerging viruses such as Ebola. Our results suggest that therapeutics targeting filamentous virus particles could overcome antiviral drug resistance and immune evasion in pleomorphic viruses.
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http://dx.doi.org/10.1038/s41564-021-00877-0DOI Listing
March 2021

Retrospective Analysis of Clinicopathological Characteristics of Lacrimal Gland Pleomorphic Adenoma and Mechanism of Tumorigenesis by the Imbalance Between Apoptosis and Proliferation.

Med Sci Monit 2021 Mar 19;27:e929152. Epub 2021 Mar 19.

Department of Ophthalmology, The Affiliated Hospital of Chengde Medical University, Chengde, Hebei, China (mainland).

BACKGROUND Lacrimal gland pleomorphic adenoma (LGPA) is the most common clinically benign epithelial tumor of the lacrimal gland and is predominantly comprised of epithelial cells and interstitial components. At present, the exact pathogenesis of LGPA remains unclear. Previous research has indicated that the occurrence of LGPA may be related to excessive cell proliferation. MATERIAL AND METHODS This study observed the clinicopathological characteristics of LGPA and investigated the tumorigenesis mechanism of cell over-proliferation caused by the imbalance between apoptosis and proliferation. A total of 27 cases were collected from the Department of Ophthalmology of the Affiliated Hospital of Chengde Medical University and the Third Medical Center of Chinese PLA General Hospital from April 2017 to November 2019. Hematoxylin-eosin (HE) staining and immunohistochemical staining were used to observe the pathological characteristics and analyze the expression of bcl-2 and bax in the lacrimal gland. RESULTS Compared with normal lacrimal gland tissues, LGPA tumor tissues had obvious changes in pathological morphology. The expression of bcl-2 in LGPA lesion tissues was dramatically higher (P<0.001), the expression of bax was not significantly different between groups (P=0.25), but the ratio of bcl-2/bax was significantly higher in tumor tissues (P=0.01). CONCLUSIONS We found that the lacrimal gland tumor tissues had obvious excessive proliferation in pathomorphology, which revealed the necessity of complete surgical removal of the capsule from the perspective of pathological morphology and provided a theoretical basis for the hypothesis that the imbalance between apoptosis and proliferation could lead to cell hyperproliferation.
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http://dx.doi.org/10.12659/MSM.929152DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7986817PMC
March 2021

A crosslinked submicro-hydrogel formed by DNA circuit-driven protein aggregation amplified fluorescence anisotropy for biomolecules detection.

Anal Chim Acta 2021 Apr 12;1154:338319. Epub 2021 Feb 12.

Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, 400715, Chongqing, PR China. Electronic address:

Protein is an excellent molecular mass amplifier without fluorescence quenching effect for fluorescence anisotropy (FA) assay. However, in traditional protein amplified FA methods, the binding ratio between amplifier and dye-modified probe is 1:1 or one target can only induce FA change of one fluorophore on probe, resulting in low sensitivity. Herein, we developed a simple FA strategy with high accuracy and sensitivity by using a crosslinked submicro-hydrogel that was formed through a catalyzed hairpin assembly (CHA) assisted protein aggregation as a novel FA amplifier. In the presence of catalyst, the CHA process was initiated through the toehold-mediated strand exchange reaction, which led to the formation of a dye and biotin-labeled Y-shaped H1-H2 duplex (YHD) and recycling of catalyst. With the introduction of streptavidin, a crosslinked submicro-hydrogel was formed by strong binding affinity between biotin on YHD and streptavidin, resulting in an increased FA of fluorescent dye. After rational design of the catalyst sequence, this method has been utilized for the detection of miRNA-145, staphylococcal enterotoxin B (SEB) and ATP with an LOD of 2.5 nM, 92 pg mL and 3.6 μM, respectively. Moreover, this FA assay has been successfully applied for direct detection of target in biological samples, demonstrating its practicality in complex biological systems.
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http://dx.doi.org/10.1016/j.aca.2021.338319DOI Listing
April 2021

Radiomics-based model using gadoxetic acid disodium-enhanced MR images: associations with recurrence-free survival of patients with hepatocellular carcinoma treated by surgical resection.

Abdom Radiol (NY) 2021 Mar 18. Epub 2021 Mar 18.

Department of Radiology, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, 510060, China.

Purpose: To develop a prediction model that combined magnetic resonance images (MRI)-based radiomics features with clinical factors to predict recurrence-free survival (RFS) of hepatocellular carcinoma (HCC) patients treated with surgical resection.

Methods: HCC patients treated with surgical resection (n = 153) were randomly divided into training (n = 107) and validation (n = 46) datasets. The volumes of interest were manually outlined around the lesion and additional 2 mm and 5 mm peritumoral areas were created with automated dilatation in MRI to extract tumoral (T) and peritumoral (PT) radiomics features. The radiomics models were constructed using least absolute shrinkage and selection operator Cox regression. The combined model incorporated clinical factors and radiomics features using multivariable Cox regression based on the Akaike information criterion principle. Predictive performance of different models were evaluated by receiver operating characteristic (ROC) curves, decision curves, and calibration curves.

Results: Among the radiomics models, similar performance was observed in the 2 mm and 5 mm PT models (C-index both 0.657), which were better than the T model or T + PT model (C-index 0.607 and 0.641, respectively) in the validation dataset, whereas the model combined with the three identified clinical risk factors showed the best performance (C-index 0.725). Results of the ROC curves, decision curves, and the calibration curves indicated that the combined model and the derived nomogram had better prediction performance, greater clinical benefits, and fair calibration efficiency.

Conclusion: The prediction model that combined MRI radiomics signatures with clinical factors can effectively predict the prognosis of patients with HCC treated with surgical resection.
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http://dx.doi.org/10.1007/s00261-021-03034-7DOI Listing
March 2021

A DARPin targeting activated Mac-1 is a novel diagnostic tool and potential anti-inflammatory agent in myocarditis, sepsis and myocardial infarction.

Basic Res Cardiol 2021 Mar 15;116(1):17. Epub 2021 Mar 15.

Cardiology and Angiology I, Heart Center Freiburg University, Faculty of Medicine, University of Freiburg, Freiburg, Germany.

The monocyte β-integrin Mac-1 is crucial for leukocyte-endothelium interaction, rendering it an attractive therapeutic target for acute and chronic inflammation. Using phage display, a Designed-Ankyrin-Repeat-Protein (DARPin) was selected as a novel binding protein targeting and blocking the α I-domain, an activation-specific epitope of Mac-1. This DARPin, named F7, specifically binds to activated Mac-1 on mouse and human monocytes as determined by flow cytometry. Homology modelling and docking studies defined distinct interaction sites which were verified by mutagenesis. Intravital microscopy showed reduced leukocyte-endothelium adhesion in mice treated with this DARPin. Using mouse models of sepsis, myocarditis and ischaemia/reperfusion injury, we demonstrate therapeutic anti-inflammatory effects. Finally, the activated Mac-1-specific DARPin is established as a tool to detect monocyte activation in patients receiving extra-corporeal membrane oxygenation, as well as suffering from sepsis and ST-elevation myocardial infarction. The activated Mac-1-specific DARPin F7 binds preferentially to activated monocytes, detects inflammation in critically ill patients, and inhibits monocyte and neutrophil function as an efficient new anti-inflammatory agent.
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http://dx.doi.org/10.1007/s00395-021-00849-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7960600PMC
March 2021

High anti-Müllerian hormone levels might not reflect the likelihood of clinical pregnancy rate in IVF/ICSI treatment.

JBRA Assist Reprod 2020 Nov 5. Epub 2020 Nov 5.

Reproductive Medical Center, Department of Obstetrics and Gynecology, Peking University International Hospital, Beijing, 102206, China.

Objective: To investigate if high anti-Müllerian hormone (AMH) concentration is a useful tool to predict the outcome of assisted reproductive treatment.

Methods: Retrospective cohort study involving 520 patients who underwent IVF/ICSI procedures in a university hospital. We measured the serum AMH level on day 3 of the menstrual cycle. Based on AMH levels, we divided the patients into three groups as follows: low (<25th percentile) AMH group, average (25th to 75th percentile) AMH group and high (>75th percentile) AMH group. We recorded the fertilization rate (FR), the number of oocytes retrieved, the number of good quality embryos (GQEs) and the clinical pregnancy rate (CPR).

Results: There was no difference between the three AMH groups in terms of maternal age, body mass index (BMI), follicle-stimulating hormone (FSH), estradiol (E2), luteinizing hormone (LH) and testosterone (T) in the IVF/ICSI cycles. The women in the high serum AMH group had a higher number of retrieved oocytes than those in the low or average AMH groups (p < 0.01) in the IVF/ICSI cycles. Compared with the low or average AMH groups, the women with high AMH levels had a higher number of good quality embryos (GQEs) in the IVF/ICSI cycles (p < 0.01). However, high AMH women had no significantly higher clinical pregnancy rate (CPR) compared to the women in the low or average AMH groups. In addition, for the prediction of CPR, the AMH levels alone were not an independent predictor of CPR for IVF and ICSI cycles in the ROC curve analysis.

Conclusions: High anti-Müllerian hormone levels are an independent predictor of the number of retrieved oocytes and good quality embryos (GQEs), but might not reflect the likelihood of higher clinical pregnancy rates (CPR) in IVF/ICSI treatment.
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http://dx.doi.org/10.5935/1518-0557.20200094DOI Listing
November 2020