Publications by authors named "Tian He"

703 Publications

Glutathione-modified macrophage-derived cell membranes encapsulated metformin nanogels for the treatment of spinal cord injury.

Mater Sci Eng C Mater Biol Appl 2022 Jan 18:112668. Epub 2022 Jan 18.

Pharmacy School, Jinzhou Medical University, Jinzhou 121001, China. Electronic address:

Spinal cord injury (SCI) causes a range of pathological responses, including oxidative stress, inflammation and apoptosis. In SCI treatment, whether an effective drug preparation can cross the blood-spinal cord barrier (BSCB) to the injury site is closely related to its therapeutic effect. Metformin (Met) is a glucose-lowering drug that shows a good effect for the treatment of SCI. However, it cannot cross the BSCB, which limits its application. In this study, we prepared glutathione-modified macrophage-derived cell membranes encapsulating metformin nanogels (Met-CNG-GSH) to solve this problem. Drug release and pharmacokinetics study results indicated that Met-CNG-GSH exhibits a slow release effect, and in vivo imaging demonstrated that Met-CNG-GSHs accumulated at the injury site, indicating that it has a good targeting effect. Animal experiments demonstrated that Met-CNG-GSH has a good therapeutic effect in alleviating oxidative stress, inflammation, and apoptosis. Therefore, Met-CNG-GSH represents a potential treatment for SCI.
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http://dx.doi.org/10.1016/j.msec.2022.112668DOI Listing
January 2022

Dynamic timing control over multicolor molecular emission by temporal chemical locking.

Angew Chem Int Ed Engl 2022 Jan 24. Epub 2022 Jan 24.

Key Labs for Advanced Materials, Institute of Fine Chemicals, East China University of Science and Technology, Meilong Road 130, 200237, Shanghai, CHINA.

Dynamic control over molecular emission, especially in a time-dependent manner, holds a great promise for the development of smart luminescent materials. Here we report a series of dynamic multicolor fluorescent systems based on the time-encoded locking and unlocking of individual vibrational emissive units. The intramolecular cyclization reaction driven by adding chemical fuel acts as a chemical lock to decrease the conformational freedom of the emissive units, thus varying the fluorescence wavelength, while the resulting chemically locked state can be automatically unlocked by the hydrolysis reaction with water molecules. The dynamic molecular system can be driven by adding chemical fuels for multiple times. The emission wavelength and lifetime of the locking states can be readily controlled by elaborating the molecular structures, indicating this strategy as a robust and versatile way to modulate multi-color molecular emission in a time-encoded manner.
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http://dx.doi.org/10.1002/anie.202116414DOI Listing
January 2022

Effects of dietary starch sources on pellet-processing characteristics, growth performance and caecal microflora of meat rabbits.

J Anim Physiol Anim Nutr (Berl) 2022 Jan 20. Epub 2022 Jan 20.

College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, Liaoning Province, China.

This study evaluated the effects of starch sources on pellet-processing characteristics as well as the growth performance and caecal microflora of rabbits. Ninety-six 35-day-old rabbits were randomly allocated to four groups with 24 rabbits per group and were fed diets with different starch sources (corn, wheat, potato or pea starch). The trial lasted for 40 days. The greatest hardness and lowest powder ratio of feed pellets was associated with the use of potato starch (p > 0.05). Pellet bulk density was the highest with corn starch, and the density was greater than that of pea starch by 5.91% (p < 0.05). The pulverisation ratio of corn starch pellets was the lowest, 43.67% lower than that of the pea starch pellets (p < 0.05). The average daily gain of rabbits in the corn starch group was higher than in the potato and pea starch groups, by 7.89% and 10.81%, respectively (p < 0.05). Rabbits in the corn starch group had the best feed conversion ratio (p > 0.05). The feed intake of rabbits in the potato starch group was higher than in the wheat and pea starch groups, by 4.30% and 5.16% respectively (p < 0.05). The dominant caecal bacteria phyla were Firmicutes, Bacteroidetes, Verrucomicrobia and Proteobacteria. There were 12 bacterial genera with proportions greater than 0.1%. The caecal proportion of Clostridium in the pea starch group was 1.8%, which was higher than those of the other groups (p = 0.057). There was no significant difference in caecal microbial diversity among groups (p > 0.05). The highest microbial clustering effect was found in the corn starch treatment. In conclusion, the best pellet quality was found using potato starch; for rabbit growth, the optimal source was corn starch.
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http://dx.doi.org/10.1111/jpn.13682DOI Listing
January 2022

Development and validation of a novel risk score to predict 5-year mortality in patients with acute myocardial infarction in China: a retrospective study.

PeerJ 2022 4;9:e12652. Epub 2022 Jan 4.

Department of Cardiology, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China.

Background: The disease burden from ischaemic heart disease remains heavy in the Chinese population. Traditional risk scores for estimating long-term mortality in patients with acute myocardial infarction (AMI) have been developed without sufficiently considering advances in interventional procedures and medication. The goal of this study was to develop a risk score comprising clinical parameters and intervention advances at hospital admission to assess 5-year mortality in AMI patients in a Chinese population.

Methods: We performed a retrospective observational study on 2,722 AMI patients between January 2013 and December 2017. Of these patients, 1,471 patients from Changsha city, Hunan Province, China were assigned to the development cohort, and 1,251 patients from Xiangtan city, Hunan Province, China, were assigned to the validation cohort. Forty-five candidate variables assessed at admission were screened using least absolute shrinkage and selection operator, stepwise backward regression, and Cox regression methods to construct the C2ABS2-GLPK score, which was graded and stratified using a nomogram and X-tile. The score was internally and externally validated. The C-statistic and Hosmer-Lemeshow test were used to assess discrimination and calibration, respectively.

Results: From the 45 candidate variables obtained at admission, 10 potential predictors, namely, including reatinine, experience of ardiac arrest, ge, N-terminal Pro-rain Natriuretic Peptide, a history of troke, tatins therapy, fasting blood lucose, eft ventricular end-diastolic diameter, ercutaneous coronary intervention and illip classification were identified as having a close association with 5-year mortality in patients with AMI and collectively termed the C2ABS2-GLPK score. The score had good discrimination (C-statistic = 0.811, 95% confidence intervals (CI) [0.786-0.836]) and calibration (calibration slope = 0.988) in the development cohort. In the external validation cohort, the score performed well in both discrimination (C-statistic = 0.787, 95% CI [0.756-0.818]) and calibration (calibration slope = 0.976). The patients were stratified into low- (≤148), medium- (149 to 218) and high-risk (≥219) categories according to the C2ABS2-GLPK score. The predictive performance of the score was also validated in all subpopulations of both cohorts.

Conclusion: The C2ABS2-GLPK score is a Chinese population-based risk assessment tool to predict 5-year mortality in AMI patients based on 10 variables that are routinely assessed at admission. This score can assist physicians in stratifying high-risk patients and optimizing emergency medical interventions to improve long-term survival in patients with AMI.
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http://dx.doi.org/10.7717/peerj.12652DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8740514PMC
January 2022

ERAP2 Is Associated With Immune Infiltration and Predicts Favorable Prognosis in SqCLC.

Front Immunol 2021 21;12:788985. Epub 2021 Dec 21.

Department of Thoracic Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.

Background: Immunotherapy has been proven effective among several human cancer types, including Squamous cell lung carcinoma (SqCLC). ERAP2 plays a pivotal role in peptide trimming of many immunological processes. However, the prognostic role of ERAP2 and its relationship with immune cell infiltration in SqCLC remains unclear.

Methods: The differential expression of ERAP2 was identified GEO and TCGA databases. We calculated the impact of ERAP2 on clinical prognosis using the Kaplan-Meier plotter. TIMER was applied to evaluate the abundance of immune cells infiltration and immune markers. SqCLC tissue microarrays containing 190 patients were constructed, and we performed immunohistochemical staining for ERAP2, CD8, CD47, CD68, and PD-L1 to validate our findings in public data.

Results: In the GEO SqCLC database, ERAP2 was upregulated in patients with better survival (p=0.001). ERAP2 expression in SqCLC was significantly lower than that of matched normal samples (p<0.05) based on TCGA SqCLC data. Higher expression of ERAP2 was significantly associated with better survival in SqCLC patients from TCGA (p=0.007), KM-plotter (p=0.017), and our tissue microarrays (TMAs) (p=0.026). In univariate and multivariate Cox analysis of SqCLC TMAs, high ERAP2 expression was identified as an independent protective factor for SqCLC patients (Univariate Cox, HR=0.659, range 0.454-0.956, p<0.05. Multivariate Cox, HR=0.578, range 0.385-0.866, p<0.05). In TIMER, ERAP2 was positively correlated with several immune markers (CD274, p=1.27E-04; CD68, p=5.88E-08) and immune infiltrating cells (CD8 T cell, p=4.09E-03; NK cell, p=1.00E-04). In our cohort, ERAP2 was significantly correlated with CD8 tumor-infiltrating lymphocytes (TILs) (p=0.0029), and patients with higher ERAP2 expression had a higher percentage of PD-L1 positive patients (p=0.049) and a higher CD8 TILs level (p=0.036).

Conclusions: For the first time, our study demonstrates that higher expression of ERAP2 is tightly associated with the immuno-supportive microenvironment and can predict a favorable prognosis in SqCLC. Meanwhile, ERAP2 may be a promising immunotherapeutic target for patients with SqCLC.
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http://dx.doi.org/10.3389/fimmu.2021.788985DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8725995PMC
December 2021

Disulfide-Mediated Reversible Polymerization toward Intrinsically Dynamic Smart Materials.

J Am Chem Soc 2022 Jan 6. Epub 2022 Jan 6.

Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, Frontiers Science Center for Materiobiology and Dynamic Chemistry, Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China.

The development of a dynamic chemistry toolbox to endow materials dynamic behavior has been key to the rational design of future smart materials. The rise of supramolecular and dynamic covalent chemistry offers many approaches to the construction of dynamic polymers and materials that can adapt, respond, repair, and recycle. Within this toolbox, the building blocks based on 1,2-dithiolanes have become an important scaffold, featuring their reversible polymerization mediated by dynamic covalent disulfide bonds, which enables a unique class of dynamic materials at the intersection of supramolecular polymers and adaptable covalent networks. This Perspective aims to explore the dynamic chemistry of 1,2-dithiolanes as a versatile structural unit for the design of smart materials by summarizing the state of the art as well as providing an overview of the fundamental challenges involved in this research area and its potential future directions.
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http://dx.doi.org/10.1021/jacs.1c10359DOI Listing
January 2022

Photoresponsive DNA materials and their applications.

Chem Soc Rev 2022 Jan 24;51(2):720-760. Epub 2022 Jan 24.

Institute of Chemistry, The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.

Photoresponsive nucleic acids attract growing interest as functional constituents in materials science. Integration of photoisomerizable units into DNA strands provides an ideal handle for the reversible reconfiguration of nucleic acid architectures by light irradiation, triggering changes in the chemical and structural properties of the nanostructures that can be exploited in the development of photoresponsive functional devices such as machines, origami structures and ion channels, as well as environmentally adaptable 'smart' materials including nanoparticle aggregates and hydrogels. Moreover, photoresponsive DNA components allow control over the composition of dynamic supramolecular ensembles that mimic native networks. Beyond this, the modification of nucleic acids with photosensitizer functionality enables these biopolymers to act as scaffolds for spatial organization of electron transfer reactions mimicking natural photosynthesis. This review provides a comprehensive overview of these exciting developments in the design of photoresponsive DNA materials, and showcases a range of applications in catalysis, sensing and drug delivery/release. The key challenges facing the development of the field in the coming years are addressed, and exciting emergent research directions are identified.
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http://dx.doi.org/10.1039/d1cs00688fDOI Listing
January 2022

Corrigendum to 'Berberine-loaded M2 macrophage-derived exosomes for spinal cord injury therapy' Acta Biomaterialia 126 (2021) 211-223.

Acta Biomater 2021 Dec 27. Epub 2021 Dec 27.

Pharmacy School, Jinzhou Medical University, Jinzhou, China. Electronic address:

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http://dx.doi.org/10.1016/j.actbio.2021.12.002DOI Listing
December 2021

Functional resveratrol-biodegradable manganese doped silica nanoparticles for the spinal cord injury treatment.

Mater Today Bio 2022 Jan 4;13:100177. Epub 2021 Dec 4.

Pharmacy School, Jinzhou Medical University, Jinzhou, Liaoning, 121001, China.

Spinal cord injury (SCI) causes secondary injury, accompanied by pathological changes such as oxidative stress, inflammation and neuronal apoptosis. This leads to permanent disabilities such as paralysis and loss of movement or sensation. Due to the ineffectiveness of drugs passing through the blood spinal cord barrier (BSCB), there is currently no effective treatment for SCI. The aim of this experiment was to design plasma complex component functionalized manganese-doped silica nanoparticles (PMMSN) with a redox response as a targeted drug carrier for resveratrol (RES), which effectively transports insoluble drugs to cross the BSCB. RES was adsorbed into PMMSN with a particle size of approximately 110 ​nm by the adsorption method, and the drug loading reached 32.61 ​± ​3.38%. The RES release results for the loaded sample (PMMSN-RES) showed that the PMMSN-RES exhibited a release slowly effect. In vitro and vivo experiments demonstrated that PMMSN-RES decreased reactive oxygen species (ROS) and malondialdehyde (MDA), increased superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities, reduced the expression of inflammatory (TNF-α, IL-1β and IL-6) and apoptotic cytokines (cleaved caspase-3) in spinal cord tissue after SCI. In summary, PMMSN-RES may be a potential pharmaceutical preparation for the treatment of SCI by reducing neuronal apoptosis and inhibiting inflammation caused by reducing oxidative stress to promote the recovery of mouse motor function.
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http://dx.doi.org/10.1016/j.mtbio.2021.100177DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8661701PMC
January 2022

Comprehensive analysis of PD-L1 expression, tumor-infiltrating lymphocytes, and tumor microenvironment in LUAD: differences between Asians and Caucasians.

Clin Epigenetics 2021 12 21;13(1):229. Epub 2021 Dec 21.

Department of Thoracic Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, NO. 17, Panjiayuannanli, Chaoyang District, Beijing, 100021, China.

Backgrounds: The characteristics of programmed cell death protein-1 (PD-L1) expression, tumor-infiltrating lymphocytes (TILs), and tumor microenvironment (TME) in lung adenocarcinoma (LUAD) patients are closely related to immunotherapy, and there are differences between Asians and Caucasians.

Methods: Acquire the transcriptome data of the Cancer Genome Atlas and Chinese LUAD patients. R software was used to analyze the differential expression of genes, prognosis, and gene function. Use CIBERSORT for TIL-related analysis and ESTIMATE for TME-related analysis.

Results: The expression of PD-L1 in tumor tissues of Caucasian LUAD patients was lower than that in normal tissues, while there was no significant difference in Asians. There was no statistical difference between PD-L1 expression and prognosis. The composition of TILs between Caucasian and Asian LUAD patients was quite different. There was no correlation between TILs and prognosis in Caucasians. However, the higher content of resting mast cells indicated a better prognosis in Asians. The Caucasian patients with higher immune and estimate scores had a better prognosis (p = 0.021, p = 0.025). However, the Asian patients with a higher estimate score had a worse prognosis (p = 0.024). The high expression of COL5A2 (p = 0.046, p = 0.027) and NOX4 (p = 0.020, p = 0.019) were both associated with the poor prognosis in Caucasians and Asians.

Conclusion: There are many differences in the characteristics of PD-L1 expression, TILs, and TME between Caucasian and Asian LUAD patients. This provides a certain hint for the selection of specific immunotherapy strategies separately for Caucasian and Asian LUAD patients.
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http://dx.doi.org/10.1186/s13148-021-01221-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8693498PMC
December 2021

A case of Crohn's disease combined with inflammatory myofibroblastoma of abdominal wall.

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2021 Nov;46(11):1310-1314

Department of Gastroenterology, Third Xiangya Hospital, Central South University, Changsha 410013.

Inflammatory myofibroblastoma (IMT) is a rare solid tumor, and its etiology and pathogenesis are unclear. Crohn's disease is a non-specific intestinal inflammatory disease. The clinical manifestations, laboratory examinations, and imaging examinations of IMT are not specific, making diagnosis difficult. A case of Crohn's disease combined with IMT of abdominal wall was admitted to the Department of Gastroenterology at the Third Xiangya Hospital, Central South University, on Nov. 21, 2017. This patient was admitted to our hospital because of repeated right lower abdominal pain for 4 years. A 6 cm×5 cm mass was palpated in the right lower abdomen. After completing the transanal double-balloon enteroscopy and computed tomographic enterography for the small intestinal, the cause was still unidentified. The patient underwent surgery due to an abdominal wall mass with intestinal fistula on Sept. 12, 2018 and recovered well currently. According to histopathology and immunohistochemistry, he was diagnosed with Crohn's disease combined with IMT. Up to July 2020, the patients still took azathioprine regularly, without abdominal pain, abdominal distension, and other discomfort, and the quality of his life was good.
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http://dx.doi.org/10.11817/j.issn.1672-7347.2021.200966DOI Listing
November 2021

A Universal Strategy for Tunable Persistent Luminescent Materials via Radiative Energy Transfer.

Angew Chem Int Ed Engl 2021 Dec 13:e202115748. Epub 2021 Dec 13.

Key Laboratory for Advanced Materials and Feringa Nobel Prize Scientist Joint Research Center, Frontiers Science Center for Materiobiology and Dynamic Chemistry, School of Chemistry and Molecular Engineering, East China University of Science & Technology, Meilong Road 130, Shanghai, 200237, China.

In this work, a universal strategy for solid, solution, or gel state organic persistent luminescent materials via radiative energy transfer is proposed. The persistent luminescence (τ>0.7 s) could be remotely regulated between different colors by controlling the isomerization of the energy acceptor. The function relies on the simple radiative energy transfer (reabsorption) mechanism, rather than the complicated communication between the excited state of the molecules such as Förster resonance energy transfer or Dexter energy transfer. And the "apparent lifetime" for the energy acceptor is the same as the lifetime of the energy donor, which was different with a traditional radiative energy transfer process. The simple working principle endows this strategy with huge universality, flexibility, and operability. This work offers a simple, feasible, and universal way to construct various persistent luminescent materials in solid, solution, and gel states.
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http://dx.doi.org/10.1002/anie.202115748DOI Listing
December 2021

Intelligent and Multifunctional Graphene Nanomesh Electronic Skin with High Comfort.

Small 2021 Dec 9:e2104810. Epub 2021 Dec 9.

School of Integrated Circuits (SIC) and Beijing National Research Center for Information Science and Technology (BNRist), Tsinghua University, Beijing, 100084, China.

As the aging population increases in many countries, electronic skin (e-skin) for health monitoring has been attracting much attention. However, to realize the industrialization of e-skin, two factors must be optimized. The first is to achieve high comfort, which can significantly improve the user experience. The second is to make the e-skin intelligent, so it can detect and analyze physiological signals at the same time. In this article, intelligent and multifunctional e-skin consisting of laser-scribed graphene and polyurethane (PU) nanomesh is realized with high comfort. The e-skin can be used as a strain sensor with large measurement range (>60%), good sensitivity (GF≈40), high linearity range (60%), and excellent stability (>1000 cycles). By analyzing the morphology of e-skin, a parallel networks model is proposed to express the mechanism of the strain sensor. In addition, laser scribing is also applied to etch the insulating PU, which greatly decreases the impedance in detecting electrophysiology signals. Finally, the e-skin is applied to monitor the electrocardiogram, electroencephalogram (EEG), and electrooculogram signals. A time- and frequency-domain concatenated convolution neural network is built to analyze the EEG signal detected using the e-skin on the forehead and classify the attention level of testers.
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http://dx.doi.org/10.1002/smll.202104810DOI Listing
December 2021

Ag/Zn Galvanic Couple Cotton Nonwovens with Breath-Activated Electroactivity: A Possible Antibacterial Layer for Personal Protective Face Masks.

ACS Appl Mater Interfaces 2021 Dec 5;13(49):59196-59205. Epub 2021 Dec 5.

Industrial Research Institute of Nonwovens and Technical Textiles, College of Textiles and Clothing, Qingdao University, Qingdao 266071, P. R. China.

The water vapor exhaled by the human body can severely accelerate the charge dissipation of commercial face masks, thereby reducing the electrostatic adsorption efficiency and increasing the bacterial invasion risk. This study developed an electroactive antibacterial cotton nonwoven (Ag/cotton/Zn) using eco-friendly magnetron sputtering technology. The Ag/Zn electrode constructed on the surface of cotton nonwovens could produce a microelectric field in the moist environment of human respiration, which endowed Ag/cotton/Zn with excellent electroactivity. When Ag/cotton/Zn was used as an additional layer of polypropylene melt-blown nonwovens or polylactic acid nanofibers, the prepared personal protective air filter had a filtration efficiency of up to 96.8% and an appropriate pressure drop and air permeability. The antibacterial results based on bacterial aerosols showed that the antibacterial efficiency against and in 20 min was 99.74 and 99.79%, respectively, indicating an excellent electroactive killing efficiency against airborne bacteria. In addition, Ag/cotton/Zn showed excellent biological security. These results shed some light on the design and fabrication of next generation of personal protective air filter materials driven by human breathing.
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http://dx.doi.org/10.1021/acsami.1c15113DOI Listing
December 2021

Single-Cell Sequencing Reveals Differential Cell Types in Skin Tissues of Liaoning Cashmere Goats and Key Genes Related Potentially to the Fineness of Cashmere Fiber.

Front Genet 2021 10;12:726670. Epub 2021 Nov 10.

College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, China.

Cashmere fineness is one of the important factors determining cashmere quality; however, our understanding of the regulation of cashmere fineness at the cellular level is limited. Here, we used single-cell RNA sequencing and computational models to identify 13 skin cell types in Liaoning cashmere goats. We also analyzed the molecular changes in the development process by cell trajectory analysis and revealed the maturation process in the gene expression profile in Liaoning cashmere goats. Weighted gene co-expression network analysis explored hub genes in cell clusters related to cashmere formation. Secondary hair follicle dermal papilla cells (SDPCs) play an important role in the growth and density of cashmere. , a marker gene of SDPCs, was selected for immunofluorescence (IF) and Western blot (WB) verification. Our results indicate that ACTA2 is mainly expressed in SDPCs, and WB results show different expression levels. is a highly expressed gene in SDPCs, which was verified by IF and WB. We then selected of SDPCs to verify and prove the differential expression in the coarse and fine types of Liaoning cashmere goats. Therefore, the gene may regulate cashmere fineness. These genes may be involved in regulating the fineness of cashmere in goat SDPCs; our research provides new insights into the mechanism of cashmere growth and fineness regulation by cells.
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http://dx.doi.org/10.3389/fgene.2021.726670DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8631524PMC
November 2021

Evaluation of the therapeutic efficacy of human bone marrow mesenchymal stem cells with COX-2 silence and TGF-β3 overexpression in rabbits with antigen-induced arthritis.

Authors:
Tian He Shui Sun

Exp Cell Res 2022 01 25;410(1):112945. Epub 2021 Nov 25.

Department of Joint Surgery, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250021, China. Electronic address:

Objective: Mesenchymal stem cells (MSCs), especially genetically modified MSCs, have become a promising therapeutic approach for the treatment of rheumatoid arthritis (RA) through modulating immune responses. However, most MSCs used in the treatment of RA are modified based on a single gene. In this study, we evaluated the therapeutic effects of human BMSCs (hBMSCs) with COX-2 silence and TGF-β3 overexpression in the treatment of RA in a rabbit model.

Materials And Methods: hBMSCs were cotransfected with shCOX-2 and TGF-β3 through lentiviral vector delivery. After SPIO-Molday ION Rhodamine-B™ (MIRB) labeling, lenti-shCOX2-TGF-β3 hBMSCs, lenti-shCOX2 hBMSCs, lenti-TGF-β3 hBMSCs, hBMSCs without genetic modification, or phosphate-buffered saline (PBS) were injected into the knee joint of rabbits with antigen-induced arthritis (AIA). The diameter of the knee joint and soft-tissue swelling score (STS) were recorded, and the levels of inflammatory mediators, including interleukin-1β (IL-1β), tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6), and prostaglandin E2 (PGE2) were evaluated by ELISA. Clinical 3.0T MR imaging (MRI) was used to track the distribution and dynamic migration of hBMSCs in the joint. Histopathological and immunohistochemical assays were conducted to localize labeled hBMSCs and assess the alteration of synovial hyperplasia, inflammatory cell infiltration, and cartilage damage.

Results: COX-2 silencing and TGF-β3 overexpression in hBMSCs were confirmed through real-time PCR and Western blot analyses. Reduced joint diameter, soft-tissue swelling (STS) score, and PGE2, IL-1β, and TNF-α expression were detected 4 weeks after injection of MIRB-labeled lenti-shCOX2-TGF-β3 hBMSCs into the joint in rabbits with AIA. Eight weeks after hBMSC injection, reduced inflammatory cell infiltration, improved hyperplasia of the synovial lining, recovered cartilage damage, and increased matrix staining were observed in joints injected with lenti-shCOX2-TGF-β3 hBMSCs and lenti-shCOX2 hBMSCs. Slight synovial hyperplasia, no surface fibrillation, and strong positive expression of collagen II staining in chondrocytes and cartilage matrix were detected in the joints 12 weeks after injection of lenti-shCOX2-TGF-β3 hBMSCs. In addition, hBMSCs were detected by MRI imaging throughout the process of hBMSC treatment.

Conclusion: Intra-articular injection of hBMSCs with COX-2 silence and TGFβ3 overexpression not only significantly inhibited joint inflammation and synovium hyperplasia, but also protected articular cartilage at the early stage. In addition, intra-articular injection of hBMSCs with COX-2 silence and TGFβ3 overexpression promoted chondrocyte and matrix proliferation. This study provides an alternative therapeutic strategy for the treatment of RA using genetically modified hBMSCs.
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http://dx.doi.org/10.1016/j.yexcr.2021.112945DOI Listing
January 2022

Association of blood pressure in the first-week of hospitalization and long-term mortality in patients with acute left ventricular myocardial infarction.

Int J Cardiol 2022 Feb 24;349:18-26. Epub 2021 Nov 24.

Department of Cardiology, The Third Xiangya Hospital, Central South University, Changsha 410013, China. Electronic address:

Background: Previous studies have shown that optimal blood pressure (BP) control is necessary to outcomes in patients with acute myocardial infarction (AMI). Acute left ventricular MI is a prevalent type of AMI with poor prognosis. We aimed to analyze the associations between BP control in the first 7 days of hospitalization and long-term mortality specific to patients with isolated left ventricular MI.

Methods: A total of 3108 acute left ventricular MI patients were included in this analysis. The average BP on the first seven days of hospitalization was categorized into 10-mmHg increments. The primary and secondary outcomes were all-cause death and cardiac death, respectively. Cox models were used to assess the association of outcomes with BP during hospitalization.

Results: The median length-of-stay was 7 (IQR 6-10) days. The relationship between systolic BP (SBP) or diastolic BP (DBP) followed a U-shaped curve association with outcomes. All-cause mortality was higher in patients with lower SBP (≤90 mmHg) (adjusted hazard ratios (HRs) 7.12, 95% confidence interval (CI) 3.13-16.19; p < 0.001) and DBP (<60 mmHg) (HR 1.76, 95% CI 1.14-2.71; p = 0.011) [reference: 110 < SBP ≤120 mmHg; 70 < DBP ≤ 80 mmHg], respectively. Furthermore, primary outcome was higher in patients with higher SBP (>130 mmHg) (HR 1.51, 95% CI 1.12-2.03; p = 0.007) and DBP (>80 mmHg) (HR 1.61, 95% CI 1.20-2.18; p = 0.002), respectively.

Conclusion: Maintaining a SBP from 90 to 130 mmHg and a DBP from 60 to 80 mmHg may be beneficial to patients with acute left ventricular MI in the long run.
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http://dx.doi.org/10.1016/j.ijcard.2021.11.045DOI Listing
February 2022

Ten year trends in hospital encounters for pediatric asthma: an Indiana experience.

J Asthma 2021 Dec 7:1-10. Epub 2021 Dec 7.

Indiana University School of Medicine, Indianapolis, IN, USA.

Introduction: Pediatric asthma is a common cause of emergency department visits, hospital admissions, and mortality. Population incidence studies have historically used large-scale survey data. We measured these epidemiologic trends using a health information exchange.

Methods: In this retrospective cohort study, we used electronic health record data from a regional health information exchange to study clinical trends in pediatric patients presenting to the hospital for asthma in the State of Indiana. Data was obtained from 2010 to 2019 and included all patients ages 2-18 years. Study participants were identified using international classification of disease codes. The measured outcomes were number of hospital encounters per year, percentage of admissions per year, and mortality rates.

Results: Data included 50,393 unique patients and 88,772 unique encounters, with 57% male patients. Over the ten-year period, hospital encounters ranged from 5000 to 8000 per year with no change in trajectory. Between 2010 and 2012, the percent of encounters admitted to the hospital was ∼30%. This decreased to ∼20-25% for 2015-2019. Patient mortality rates increased from 1 to 3 per 1000 patient encounters in 2010-2014 to between 5 and 7 per 1000 patient encounters from 2016 to 2019. White patients had a significantly higher admission percentage compared to other racial groups, but no difference in mortality rate.

Conclusions: Asthma continues to be a common condition requiring hospital care for pediatric patients. Regional health information exchanges can enable public health researchers to follow asthma trends in near real time, and have potential for informing patient-level public health interventions.
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http://dx.doi.org/10.1080/02770903.2021.2010750DOI Listing
December 2021

Engineered extracellular vesicles derived from primary M2 macrophages with anti-inflammatory and neuroprotective properties for the treatment of spinal cord injury.

J Nanobiotechnology 2021 Nov 17;19(1):373. Epub 2021 Nov 17.

Department of Histology and Embryology, Jinzhou Medical University, No. 40, Songpo Road, Jinzhou, 121002, Liaoning, China.

Background: Uncontrollable inflammation and nerve cell apoptosis are the most destructive pathological response after spinal cord injury (SCI). So, inflammation suppression combined with neuroprotection is one of the most promising strategies to treat SCI. Engineered extracellular vesicles with anti-inflammatory and neuroprotective properties are promising candidates for implementing these strategies for the treatment of SCI.

Results: By combining nerve growth factor (NGF) and curcumin (Cur), we prepared stable engineered extracellular vesicles of approximately 120 nm from primary M2 macrophages with anti-inflammatory and neuroprotective properties ([email protected]). Notably, NGF was coupled with EVs by matrix metalloproteinase 9 (MMP9)-a cleavable linker to release at the injured site accurately. Through targeted experiments, we found that these extracellular vesicles could actively and effectively accumulate at the injured site of SCI mice, which greatly improved the bioavailability of the drugs. Subsequently, [email protected] reached the injured site and could effectively inhibit the uncontrollable inflammatory response to protect the spinal cord from secondary damage; in addition, [email protected] could release NGF into the microenvironment in time to exert a neuroprotective effect against nerve cell damage.

Conclusions: A series of in vivo and in vitro experiments showed that the engineered extracellular vesicles significantly improved the microenvironment after injury and promoted the recovery of motor function after SCI. We provide a new method for inflammation suppression combined with neuroprotective strategies to treat SCI.
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http://dx.doi.org/10.1186/s12951-021-01123-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8600922PMC
November 2021

A Multi-Center Validated Subtyping Model of Esophageal Cancer Based on Three Metabolism-Related Genes.

Front Oncol 2021 25;11:772145. Epub 2021 Oct 25.

Department of Thoracic Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Metabolic reprogramming is a hallmark of malignancy. Understanding the characteristics of metabolic reprogramming in esophageal squamous cell carcinoma (ESCC) helps uncover novel targets for cancer progression. In this study, 880 metabolism-related genes were identified from microarray data and then filtered to divide patients into two subgroups using consensus clustering, which exhibits significantly different overall survival. After a differential analysis between two subtypes, 3 genes were screened out to construct a two subtypes decision model on the training cohort (GSE53624), defined as high-risk and low-risk subtypes. These risk models were then verified in two public databases (GSE53622 and TCGA-ESCC), an independent cohort of 49 ESCC patients by RT-qPCR and an external cohort of 95 ESCC patients by immunohistochemistry analysis (IHC). Furthermore, the immune cell infiltration of regulatory T cells (Tregs) and plasma cells showed a significant difference between the high and low-risk subtypes in the IHC experiment with 119 ESCC patients. In conclusion, our study indicated that three metabolism-related prognostic genes could stratify patients into subgroups and were associated with immune infiltration, clinical features and clinical outcomes.
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http://dx.doi.org/10.3389/fonc.2021.772145DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8573269PMC
October 2021

Ferroptosis Characterization in Lung Adenocarcinomas Reveals Prognostic Signature With Immunotherapeutic Implication.

Front Cell Dev Biol 2021 20;9:743724. Epub 2021 Oct 20.

Department of Thoracic Surgery, National Cancer Center, National Clinical Research Center for Cancer, Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

The iron-dependent cell death named ferroptosis has been implicated in the progression and therapeutic response of several tumors. However, potential role of ferroptosis in lung adenocarcinomas (LUAD) remained less well understood. In TCGA-LUAD cohort, unsupervised clustering was first conducted based on ferroptosis regulators extracted from FerrDb database. Comprehensive correlation analysis and comparisons were performed among ferroptosis subtypes. The ferroptosis-related prognostic (FRP) signature was identified based on filtered features and repeated LASSO and was validated in five independent cohorts. The clinical relevance between the risk score and therapeutic response was further explored by multiple algorithms. qPCR was implemented to verify gene expression. A total of 1,168 LUAD patients and 161 ferroptosis regulators were included in this study. Three ferroptosis subtypes were identified and patients in subtype B had the best prognosis among the three subtypes. Significant differences in immune microenvironment and biological function enrichment were illustrated in distinct subtypes. The Boruta algorithm was conducted on 308 common differentially expressed genes for dimensionality reduction. A total of 56 genes served as input for model construction and a six-gene signature with the highest frequencies of 881 was chosen as FRP. The prognostic significance of FRP was validated in five independent cohorts. High FRP risk score was also linked to increased tumor mutation burden, PD-L1 protein expression and number of neoantigens. Of the FRP genes, 83.3% was abnormally expressed in LUAD cell lines. In conclusion, ferroptosis plays a non-negligible role in LUAD. Exploration of the ferroptosis pattern will enhance the prognostic stratification of individual patients and move toward the purpose of personalized treatment.
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http://dx.doi.org/10.3389/fcell.2021.743724DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8563998PMC
October 2021

Graphene-Based Multifunctional Textile for Sensing and Actuating.

ACS Nano 2021 Nov 1. Epub 2021 Nov 1.

School of Integrated Circuits and Beijing National Research Center for Information Science and Technology (BNRist), Tsinghua University, Beijing 100084, China.

Textiles are materials that are extensively used in everyday life; textile-based sensors can, therefore, be regarded as ideal devices for a health monitor. However, previously reported textile sensors have limited prospects due to their single function or incompatibility. Traditional textile sensors generally focus on signal detection, which has not been able to be combined with an actuator to provide real-time health status feedback. Thus, to date, there are no well-established health monitoring systems based on intelligent textiles. Herein, we present a wearable batch-prepared graphene-based textile based on laser-scribing and thermal-transfer technology. Integrated with four functions of strain sensing, pressure sensing, physiological electrical sensing, and sound emitting, the GT is able to detect human body signals and transduce them to sound signals when the user is in an abnormal physical state. Moreover, the GT has high linearity for both strain and pressure sensing; the coefficients of determination exceed 99.3% and 98.2%, respectively. The performance of the device remains stable up to a pressure of 1000 kPa. The response time of the GT possession reaches as low as 85 ms at 4.2 Pa pressure. Therefore, due to their diversified functions and good performance, the research on GT is expected to extend to the fields of health monitoring, sports monitoring, and so forth.
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http://dx.doi.org/10.1021/acsnano.1c05701DOI Listing
November 2021

H3K36 trimethylation-mediated biological functions in cancer.

Clin Epigenetics 2021 10 29;13(1):199. Epub 2021 Oct 29.

Department of Thoracic Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China.

Histone modification is an important form of epigenetic regulation. Thereinto, histone methylation is a critical determination of chromatin states, participating in multiple cellular processes. As a conserved histone methylation mark, histone 3 lysine 36 trimethylation (H3K36me3) can mediate multiple transcriptional-related events, such as the regulation of transcriptional activity, transcription elongation, pre-mRNA alternative splicing, and RNA mA methylation. Additionally, H3K36me3 also contributes to DNA damage repair. Given the crucial function of H3K36me3 in genome regulation, the roles of H3K36me3 and its sole methyltransferase SETD2 in pathogenesis, especially malignancies, have been emphasized in many studies, and it is conceivable that disruption of histone methylation regulatory network composed of "writer", "eraser", "reader", and the mutation of H3K36me3 codes have the capacity of powerfully modulating cancer initiation and development. Here we review H3K36me3-mediated biological processes and summarize the latest findings regarding its role in cancers. We highlight the significance of epigenetic combination therapies in cancers.
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http://dx.doi.org/10.1186/s13148-021-01187-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8555273PMC
October 2021

Human neutrophil peptide 1 promotes immune sterilization in vivo by reducing the virulence of multidrug-resistant Klebsiella pneumoniae and increasing the ability of macrophages.

Biotechnol Appl Biochem 2021 Oct 19. Epub 2021 Oct 19.

Emergency Department, Wuxi Second People's Hospital, Wuxi, China.

By studying the expression in patients and cell modeling in vitro, antimicrobial peptides for Klebsiella were screened. Killing curve and membrane permeability experiments are used to study the antibacterial effect of antimicrobial peptides in vitro. Cytotoxicity-related indicators including lipopolysaccharide (LPS), capsule polysaccharide (CPS), and outer membrane protein expression were measured. Intranasal inoculation of pneumoconiosis was used to construct a mouse infection model, and the survival rate and cytokine expression level were tested. Human neutrophil peptide 1 (HNP-1) showed a significant antibacterial effect, which improved the permeability of the outer membrane of K. pneumoniae. Moreover, HNP-1 decreased LPS, CPS content, and outer membrane proteins. K. pneumoniae infection decreased antimicrobial peptide, oxidative stress, and autophagy-related genes, while HNP-1 increased these genes. After coculture with macrophages, the endocytosis of macrophages is enhanced and the bacterial load is greater in the K. pneumoniae + peptide group. Besides, higher levels of pp38 and pp65 in the K. pneumoniae + peptide group. HNP-1 rescued the cytotoxicity induced by K. pneumoniae. The survival rate is significantly improved after K. pneumoniae is treated by HNP-1. All cytokines in the peptide group were significantly higher. HNP-1 promotes immune sterilization by reducing the virulence of multidrug-resistant K. pneumoniae and increasing the ability of macrophages.
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http://dx.doi.org/10.1002/bab.2270DOI Listing
October 2021

Volume and surface coil simultaneous reception (VSSR) method for intensity inhomogeneity correction in MRI.

Technol Health Care 2021 Sep 29. Epub 2021 Sep 29.

School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, Sichuan, China.

Background: Addressing intensity inhomogeneity is critical in magnetic resonance imaging (MRI) because associated errors can adversely affect post-processing and quantitative analysis of images (i.e., segmentation, registration, etc.), as well as the accuracy of clinical diagnosis. Although several prior methods have been proposed to eliminate or correct intensity inhomogeneity, some significant disadvantages have remained, including alteration of tissue contrast, poor reliability and robustness of algorithms, and prolonged acquisition time.

Objective: In this study, we propose an intensity inhomogeneity correction method based on volume and surface coils simultaneous reception (VSSR).

Methods: The VSSR method comprises of two major steps: 1) simultaneous image acquisition from both volume and surface coils and 2) denoising of volume coil images and polynomial surface fitting of bias field. Extensive in vivo experiments were performed considering various anatomical structures, acquisition sequences, imaging resolutions, and orientations. In terms of correction performance, the proposed VSSR method was comparatively evaluated against several popular methods, including multiplicative intrinsic component optimization and improved nonparametric nonuniform intensity normalization bias correction methods.

Results: Experimental results show that VSSR is more robust and reliable and does not require prolonged acquisition time with the volume coil.

Conclusion: The VSSR may be considered suitable for general implementation.
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http://dx.doi.org/10.3233/THC-213149DOI Listing
September 2021

Identification of Pulpitis-Related Potential Biomarkers Using Bioinformatics Approach.

Comput Math Methods Med 2021 29;2021:1808361. Epub 2021 Sep 29.

Department of Preventive Dentistry, Qingdao Stomatological Hospital Affiliated to Qingdao University, 266001 Qingdao, Shandong Province, China.

Inflammatory reaction of pulp tissue plays a role in the pathogen elimination and tissue repair. The evaluation of severity of pulpitis can serve an instructive function in therapeutic scheme. However, there are many limitations in the traditional evaluation methods for the severity of pulpitis. Based on the Gene Expression Omnibus (GEO) database, our study discovered 843 differentially expressed genes (DEGs) related to pulpitis. Afterwards, we constructed a protein-protein interaction (PPI) network of DEGs and used MCODE plugin to determine the key functional subset. Meanwhile, genes in the key functional subset were subjected to GO and KEGG enrichment analyses. The result showed that genes were mainly enriched in inflammatory reaction-related functions. Next, we screened out intersections of PPI network nodes and pulpitis-related genes. Then, 20 genes were obtained as seed genes. In the PPI network, 50 genes that had the highest correlation with seed genes were screened out using random walk with restart (RWR). Furthermore, 4 pulpitis-related hub genes were obtained from the intersection of the top 50 genes and genes in the key functional subset. Finally, GeneMANIA was utilized to predict genes coexpressed with hub genes, and expression levels of the 4 hub genes in normal and pulpitis groups were analyzed based on GEO data. The result demonstrated that the 4 hub genes were mainly coexpressed with chemokine-related genes and were remarkably upregulated in the pulpitis group. In short, we eventually determined 4 potential biomarkers of pulpitis.
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http://dx.doi.org/10.1155/2021/1808361DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8495466PMC
November 2021

Efficient light-emitting diodes based on oriented perovskite nanoplatelets.

Sci Adv 2021 Oct 8;7(41):eabg8458. Epub 2021 Oct 8.

Zhejiang Key Laboratory for Excited-State Materials, State Key Laboratory of Silicon Materials, Department of Chemistry, Zhejiang University, Hangzhou 310027, China.

[Figure: see text].
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http://dx.doi.org/10.1126/sciadv.abg8458DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8500509PMC
October 2021

Functionalized Iron-Nitrogen-Carbon Electrocatalyst Provides a Reversible Electron Transfer Platform for Efficient Uranium Extraction from Seawater.

Adv Mater 2021 Dec 15;33(51):e2106621. Epub 2021 Oct 15.

Department of Chemistry, University of North Texas, Denton, TX, 76201, USA.

Uranium extraction from seawater provides an opportunity for sustainable fuel supply to nuclear power plants. Herein, an adsorption-electrocatalysis strategy is demonstrated for efficient uranium extraction from seawater using a functionalized iron-nitrogen-carbon (Fe-N -C-R) catalyst, comprising N-doped carbon capsules supporting FeN single-atom sites and surface chelating amidoxime groups (R). The amidoxime groups bring hydrophilicity to the adsorbent and offer surface-specific binding sites for UO capture. The site-isolated FeN centres reduce adsorbed UO to UO . Subsequently, through electrochemical reduction of the FeN sites, unstable U(V) ions are reoxidized to U(VI) in the presence of Na resulting in the generation of solid Na O(UO ·H O) , which can easily be collected. Fe-N -C-R reduced the uranium concentration in seawater from ≈3.5 ppb to below 0.5 ppb with a calculated capacity of ≈1.2 mg g within 24 h. To the best of the knowledge, the developed system is the first to use the adsorption of uranyl ions and electrodeposition of solid Na O(UO .H O) for the extraction of uranium from seawater. The important discoveries guide technology development for the efficient extraction of uranium from seawater.
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http://dx.doi.org/10.1002/adma.202106621DOI Listing
December 2021

LINC00265 promotes the viability, proliferation, and migration of bladder cancer cells via the miR-4677-3p/FGF6 axis.

Hum Exp Toxicol 2021 Dec 30;40(12_suppl):S434-S446. Epub 2021 Sep 30.

Department of Clinical Laboratory, Lianyungang TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Lianyungang, Jiangsu, China.

Background: Bladder cancer (BCa) is a common genitourinary malignancy with higher incidence in males. Long intergenic non-protein coding RNA 265 (LINC00265) is identified as an oncogene in many malignancies, while its role in BCa development remains unknown.

Purpose: To explore the functions and mechanism of LINC00265 in BCa.

Research Design: Reverse transcription quantitative polymerase chain reaction was performed to examine LINC00265 expression in BCa cells. Cell counting kit-8 assays, colony formation assays, TdT-mediated dUTP Nick-End Labeling assays, and Transwell assays were conducted to examine BCa cell viability, proliferation, apoptosis, and migration. Luciferase reporter assays and RNA immunoprecipitation assays were carried out to explore the binding capacity between miR-4677-3p and messenger RNA fibroblast growth factor 6 (FGF6) (or LINC00265). Xenograft tumor model was established to explore the role of LINC00265 .

Results: LINC00265 was highly expressed in BCa cells. LINC00265 knockdown inhibited xenograft tumor growth and BCa cell viability, proliferation and migration while enhancing cell apoptosis. Moreover, LINC00265 interacted with miR-4677-3p to upregulate the expression of FGF6. FGF6 overexpression reversed the suppressive effect of LINC00265 knockdown on malignant phenotypes of BCa cells.

Conclusions: LINC00265 promotes the viability, proliferation, and migration of BCa cells by binding with miR-4677-3p to upregulate FGF6 expression.
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http://dx.doi.org/10.1177/09603271211043479DOI Listing
December 2021

Author Correction: Acupuncture elicits neuroprotective effect by inhibiting NAPDH oxidase-mediated reactive oxygen species production in cerebral ischaemia.

Sci Rep 2021 Sep 27;11(1):19570. Epub 2021 Sep 27.

Acupuncture and Moxibustion Department, Beijing Hospital of Traditional Chinese Medicine Affiliated To Capital Medical University, 23 Meishuguanhou Street, Dongcheng District, Beijing, 100010, China.

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http://dx.doi.org/10.1038/s41598-021-98780-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8476527PMC
September 2021
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