Thomas R Cech

Thomas R Cech

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Thomas R Cech

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Regulation of histone methylation by automethylation of PRC2.

Genes Dev 2019 Oct 5;33(19-20):1416-1427. Epub 2019 Sep 5.

Howard Hughes Medical Institute, University of Colorado at Boulder, Boulder, Colorado 80309, USA.

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http://genesdev.cshlp.org/lookup/doi/10.1101/gad.328849.119
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http://dx.doi.org/10.1101/gad.328849.119DOI Listing
October 2019

Single-cell imaging reveals unexpected heterogeneity of telomerase reverse transcriptase expression across human cancer cell lines.

Proc Natl Acad Sci U S A 2019 Sep 26;116(37):18488-18497. Epub 2019 Aug 26.

Department of Biochemistry, University of Colorado Boulder, Boulder, CO 80303;

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http://dx.doi.org/10.1073/pnas.1908275116DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6744858PMC
September 2019

In Crystallo Selection to Establish New RNA Crystal Contacts.

Structure 2018 09 14;26(9):1275-1283.e3. Epub 2018 Jun 14.

Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA. Electronic address:

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https://linkinghub.elsevier.com/retrieve/pii/S09692126183016
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http://dx.doi.org/10.1016/j.str.2018.05.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6125213PMC
September 2018

Fission yeast telosomes: non-canonical histone-containing chromatin structures dependent on shelterin and RNA.

Nucleic Acids Res 2018 09;46(17):8865-8875

Telomere Biology Laboratory, Cancer Research UK, London Research Institute, London, WC2A 3LY, UK.

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http://dx.doi.org/10.1093/nar/gky605DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6158490PMC
September 2018

A Lifelong Passion for All Things Ribonucleic.

Authors:
Thomas R Cech

Cell 2018 09 11;175(1):14-17. Epub 2018 Sep 11.

BioFrontiers Institute, Department of Biochemistry, and Howard Hughes Medical Institute, University of Colorado, Boulder, CO 80309, USA. Electronic address:

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http://dx.doi.org/10.1016/j.cell.2018.08.037DOI Listing
September 2018

Live-cell imaging reveals the dynamics of PRC2 and recruitment to chromatin by SUZ12-associated subunits.

Genes Dev 2018 06 11;32(11-12):794-805. Epub 2018 Jun 11.

BioFrontiers Institute, University of Colorado at Boulder, Boulder, Colorado 80303, USA.

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http://genesdev.cshlp.org/lookup/doi/10.1101/gad.311936.118
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http://dx.doi.org/10.1101/gad.311936.118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6049511PMC
June 2018

Dynamics of human telomerase recruitment depend on template- telomere base pairing.

Mol Biol Cell 2018 Jan 31. Epub 2018 Jan 31.

Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, BioFrontiers Institute, University of Colorado Boulder, Boulder, CO 80309, USA

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http://dx.doi.org/10.1091/mbc.E17-11-0637DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5905299PMC
January 2018

Molecular analysis of PRC2 recruitment to DNA in chromatin and its inhibition by RNA.

Nat Struct Mol Biol 2017 Dec 23;24(12):1028-1038. Epub 2017 Oct 23.

Department of Chemistry & Biochemistry, BioFrontiers Institute, University of Colorado Boulder, Boulder, Colorado, USA.

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http://dx.doi.org/10.1038/nsmb.3487DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5771497PMC
December 2017

Allele-Specific DNA Methylation and Its Interplay with Repressive Histone Marks at Promoter-Mutant TERT Genes.

Cell Rep 2017 12;21(13):3700-3707

BioFrontiers Institute, Department of Chemistry and Biochemistry, and Howard Hughes Medical Institute, University of Colorado Boulder, Boulder, CO 80303, USA. Electronic address:

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http://dx.doi.org/10.1016/j.celrep.2017.12.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5747321PMC
December 2017

Conserved RNA-binding specificity of polycomb repressive complex 2 is achieved by dispersed amino acid patches in EZH2.

Elife 2017 11 29;6. Epub 2017 Nov 29.

Cecil H. and Ida Green Center for Reproductive Biology Sciences, Division of Basic Research, Department of Obstetrics and Gynecology, UT Southwestern Medical Center, Dallas, United States.

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http://dx.doi.org/10.7554/eLife.31558DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5706960PMC
November 2017

Reconstitution of human shelterin complexes reveals unexpected stoichiometry and dual pathways to enhance telomerase processivity.

Nat Commun 2017 10 20;8(1):1075. Epub 2017 Oct 20.

Howard Hughes Medical Institute, University of Colorado BioFrontiers Institute, Boulder, CO, 80309, USA.

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http://dx.doi.org/10.1038/s41467-017-01313-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5651854PMC
October 2017

How do lncRNAs regulate transcription?

Sci Adv 2017 09 27;3(9):eaao2110. Epub 2017 Sep 27.

Department of Chemistry and Biochemistry, University of Colorado BioFrontiers Institute, and Howard Hughes Medical Institute, University of Colorado, Boulder, CO 80309, USA.

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http://dx.doi.org/10.1126/sciadv.aao2110DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5617379PMC
September 2017

Targeting of Polycomb Repressive Complex 2 to RNA by Short Repeats of Consecutive Guanines.

Mol Cell 2017 Mar;65(6):1056-1067.e5

Biomedicine Discovery Institute, Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton, VIC 3800, Australia; Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC 3800, Australia; EMBL-Australia and the ARC Centre of Excellence in Advanced Molecular Imaging, Clayton, VIC 3800, Australia. Electronic address:

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http://dx.doi.org/10.1016/j.molcel.2017.02.003DOI Listing
March 2017

Targeted CRISPR disruption reveals a role for RNase MRP RNA in human preribosomal RNA processing.

Genes Dev 2017 01 23;31(1):59-71. Epub 2017 Jan 23.

Department of Chemistry and Biochemistry, BioFrontiers Institute, University of Colorado at Boulder, Boulder, Colorado 80302, USA.

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http://dx.doi.org/10.1101/gad.286963.116DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5287113PMC
January 2017

Live Cell Imaging Reveals the Dynamics of Telomerase Recruitment to Telomeres.

Cell 2016 Aug 11;166(5):1188-1197.e9. Epub 2016 Aug 11.

Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, BioFrontiers Institute, University of Colorado Boulder, Boulder, CO 80309, USA. Electronic address:

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http://dx.doi.org/10.1016/j.cell.2016.07.033DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5743434PMC
August 2016

Inhibition of telomerase RNA decay rescues telomerase deficiency caused by dyskerin or PARN defects.

Nat Struct Mol Biol 2016 Apr 7;23(4):286-92. Epub 2016 Mar 7.

Department of Chemistry &Biochemistry, University of Colorado, Boulder, Colorado, USA.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4830462PMC
http://dx.doi.org/10.1038/nsmb.3184DOI Listing
April 2016

Biochemical Properties and Biological Functions of FET Proteins.

Annu Rev Biochem 2015 8;84:355-79. Epub 2014 Dec 8.

Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, and BioFrontiers Institute, University of Colorado, Boulder, Colorado 80309; email: , ,

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http://www.annualreviews.org/doi/10.1146/annurev-biochem-060
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http://dx.doi.org/10.1146/annurev-biochem-060614-034325DOI Listing
December 2015

The recruitment of chromatin modifiers by long noncoding RNAs: lessons from PRC2.

RNA 2015 Dec;21(12):2007-22

BioFrontiers Institute and Howard Hughes Medical Institute, University of Colorado, Boulder, Colorado 80309, USA.

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http://dx.doi.org/10.1261/rna.053918.115DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4647455PMC
December 2015

Mutation of the TERT promoter, switch to active chromatin, and monoallelic TERT expression in multiple cancers.

Genes Dev 2015 Nov 29;29(21):2219-24. Epub 2015 Oct 29.

Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309, USA; Howard Hughes Medical Institute, University of Colorado BioFrontiers Institute, Boulder, Colorado 80309, USA; University of Colorado Comprehensive Cancer Center, Aurora, Colorado 80045, USA;

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http://dx.doi.org/10.1101/gad.269498.115DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4647555PMC
November 2015

A novel two-step genome editing strategy with CRISPR-Cas9 provides new insights into telomerase action and TERT gene expression.

Genome Biol 2015 Nov 10;16:231. Epub 2015 Nov 10.

Howard Hughes Medical Institute, University of Colorado BioFrontiers Institute, Boulder, CO, USA.

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http://dx.doi.org/10.1186/s13059-015-0791-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4640169PMC
November 2015

Protein-RNA interaction restricts telomerase from running through the stop sign.

Nat Struct Mol Biol 2015 Nov;22(11):835-6

Department of Molecular, Cellular and Developmental Biology, BioFrontiers Institute, and the Howard Hughes Medical Institute, University of Colorado, Boulder, Colorado, USA.

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http://dx.doi.org/10.1038/nsmb.3118DOI Listing
November 2015

Nucleic acid-binding specificity of human FUS protein.

Nucleic Acids Res 2015 Sep 6;43(15):7535-43. Epub 2015 Jul 6.

Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, BioFrontiers Institute, University of Colorado, Boulder CO 80309, USA

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http://dx.doi.org/10.1093/nar/gkv679DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551922PMC
September 2015

Human telomerase: biogenesis, trafficking, recruitment, and activation.

Genes Dev 2015 Jun;29(11):1095-105

Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, BioFrontiers Institute, University of Colorado Boulder, Boulder, Colorado 80309, USA

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http://dx.doi.org/10.1101/gad.263863.115DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4470279PMC
June 2015

RNA World research-still evolving.

Authors:
Thomas R Cech

RNA 2015 Apr;21(4):474-5

Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado BioFrontiers Institute, Boulder, Colorado 80309-0596, USA

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http://dx.doi.org/10.1261/rna.049965.115DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4371241PMC
April 2015

Contributions of the TEL-patch amino acid cluster on TPP1 to telomeric DNA synthesis by human telomerase.

J Mol Biol 2015 Mar 23;427(6 Pt B):1291-1303. Epub 2015 Jan 23.

Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, BioFrontiers Institute, University of Colorado Boulder, Boulder, CO 80309, USA. Electronic address:

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http://dx.doi.org/10.1016/j.jmb.2015.01.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4355236PMC
March 2015

Toward a consensus on the binding specificity and promiscuity of PRC2 for RNA.

Mol Cell 2015 Feb 15;57(3):552-8. Epub 2015 Jan 15.

Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, BioFrontiers Institute, University of Colorado, Boulder, CO 80309, USA. Electronic address:

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http://dx.doi.org/10.1016/j.molcel.2014.12.017DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4320675PMC
February 2015

Cancer. TERT promoter mutations and telomerase reactivation in urothelial cancer.

Science 2015 Feb 5;347(6225):1006-10. Epub 2015 Feb 5.

Howard Hughes Medical Institute, University of Colorado BioFrontiers Institute, Boulder, CO 80309, USA. Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309, USA. Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309, USA. University of Colorado Comprehensive Cancer Center, Aurora, CO 80045, USA.

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http://dx.doi.org/10.1126/science.1260200DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4640672PMC
February 2015

Disease mutant analysis identifies a new function of DAXX in telomerase regulation and telomere maintenance.

J Cell Sci 2015 Jan 21;128(2):331-41. Epub 2014 Nov 21.

Key Laboratory of Gene Engineering of the Ministry of Education and State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China Verna and Marrs Department of Biochemistry and Molecular biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA

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http://dx.doi.org/10.1242/jcs.159467DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4294776PMC
January 2015

Identification of human TERT elements necessary for telomerase recruitment to telomeres.

Elife 2014 Oct 1;3. Epub 2014 Oct 1.

Department of Chemistry and Biochemistry, BioFrontiers Institute, Howard Hughes Medical Institute, University of Colorado Boulder, Boulder, United States.

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http://dx.doi.org/10.7554/eLife.03563DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4359370PMC
October 2014

Academia and industry: Companies on campus.

Nature 2014 Oct;514(7522):297-8

University of Colorado Boulder and director of the BioFrontiers Institute.

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http://dx.doi.org/10.1038/514297aDOI Listing
October 2014

FUS is sequestered in nuclear aggregates in ALS patient fibroblasts.

Mol Biol Cell 2014 Sep 9;25(17):2571-8. Epub 2014 Jul 9.

Howard Hughes Medical Institute, Department of Chemistry and Biochemistry and BioFrontiers Institute, University of Colorado, Boulder, CO 80309

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http://www.molbiolcell.org/doi/10.1091/mbc.e14-05-1007
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http://dx.doi.org/10.1091/mbc.E14-05-1007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4148247PMC
September 2014

A dimeric state for PRC2.

Nucleic Acids Res 2014 Aug 3;42(14):9236-48. Epub 2014 Jul 3.

Department of Chemistry & Biochemistry, Howard Hughes Medical Institute, University of Colorado BioFrontiers Institute, Boulder, CO 80309-0596, USA

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http://dx.doi.org/10.1093/nar/gku540DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4132707PMC
August 2014

Inventory of telomerase components in human cells reveals multiple subpopulations of hTR and hTERT.

Nucleic Acids Res 2014 Jul 2;42(13):8565-77. Epub 2014 Jul 2.

University of Colorado BioFrontiers Institute, Boulder, CO 80303, USA Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309, USA Howard Hughes Medical Institute and Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80303, USA

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http://dx.doi.org/10.1093/nar/gku560DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4117779PMC
July 2014

The noncoding RNA revolution-trashing old rules to forge new ones.

Cell 2014 Mar;157(1):77-94

Howard Hughes Medical Institute; Department of Molecular Biophysics and Biochemistry, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, CT 06536, USA.

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http://dx.doi.org/10.1016/j.cell.2014.03.008DOI Listing
March 2014

Telomerase in bladder cancer: back to a better future?

Eur Urol 2014 Feb 28;65(2):370-1. Epub 2013 Oct 28.

University of Colorado Comprehensive Cancer Center, Aurora, CO, USA; Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, BioFrontiers Institute, University of Colorado, Boulder, CO, USA.

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http://dx.doi.org/10.1016/j.eururo.2013.10.019DOI Listing
February 2014

Promiscuous RNA binding by Polycomb repressive complex 2.

Nat Struct Mol Biol 2013 Nov 29;20(11):1250-7. Epub 2013 Sep 29.

1] Department of Chemistry & Biochemistry, Howard Hughes Medical Institute, University of Colorado Boulder, Boulder, Colorado, USA. [2] BioFrontiers Institute, University of Colorado Boulder, Boulder, Colorado, USA.

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http://dx.doi.org/10.1038/nsmb.2679DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3823624PMC
November 2013

RNA seeds higher-order assembly of FUS protein.

Cell Rep 2013 Nov 21;5(4):918-25. Epub 2013 Nov 21.

Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, BioFrontiers Institute, University of Colorado, Boulder, CO 80309, USA. Electronic address:

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http://dx.doi.org/10.1016/j.celrep.2013.11.017DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3925748PMC
November 2013

Many disease-associated variants of hTERT retain high telomerase enzymatic activity.

Nucleic Acids Res 2013 Oct 30;41(19):8969-78. Epub 2013 Jul 30.

Department of Chemistry and Biochemistry, University of Colorado BioFrontiers Institute, Boulder, CO 80309-0596, USA, Howard Hughes Medical Institute, University of Colorado, Boulder, CO 80309-0596, USA and Department of Chemistry and Biochemistry, DePauw University, Greencastle, IN 46135, USA.

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http://dx.doi.org/10.1093/nar/gkt653DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3799428PMC
October 2013

3' terminal diversity of MRP RNA and other human noncoding RNAs revealed by deep sequencing.

BMC Mol Biol 2013 Sep 21;14:23. Epub 2013 Sep 21.

Department of Chemistry and Biochemistry, BioFrontiers Institute, University of Colorado, Boulder, CO, USA.

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http://dx.doi.org/10.1186/1471-2199-14-23DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3849073PMC
September 2013

RNA recognition by the DNA end-binding Ku heterodimer.

RNA 2013 Jun 22;19(6):841-51. Epub 2013 Apr 22.

Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado BioFrontiers Institute, Boulder, Colorado 80309-0596, USA.

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http://dx.doi.org/10.1261/rna.038703.113DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3683917PMC
June 2013

Finding the end: recruitment of telomerase to telomeres.

Nat Rev Mol Cell Biol 2013 Feb 9;14(2):69-82. Epub 2013 Jan 9.

Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, BioFrontiers Institute, University of Colorado, Boulder, Colorado 80309-0596, USA.

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http://www.nature.com/articles/nrm3505
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http://dx.doi.org/10.1038/nrm3505DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3805138PMC
February 2013

How a chemist looks at RNA.

Authors:
Thomas R Cech

Angew Chem Int Ed Engl 2013 Jan 3;52(1):75-8. Epub 2012 Dec 3.

University of Colorado BioFrontiers Institute, Boulder, CO 80309-0596, USA.

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http://dx.doi.org/10.1002/anie.201205427DOI Listing
January 2013

The TEL patch of telomere protein TPP1 mediates telomerase recruitment and processivity.

Nature 2012 Dec 24;492(7428):285-9. Epub 2012 Oct 24.

University of Colorado BioFrontiers Institute, Boulder, Colorado 80309, USA.

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http://dx.doi.org/10.1038/nature11648DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3521872PMC
December 2012

SnapShot: telomeres and telomerase.

Cell 2012 Nov;151(5):1138-1138.e1

HHMI and Department of Chemistry and Biochemistry, BioFrontiers Institute, University of Colorado, Boulder, CO 80309, USA.

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http://dx.doi.org/10.1016/j.cell.2012.11.008DOI Listing
November 2012

The RNA worlds in context.

Authors:
Thomas R Cech

Cold Spring Harb Perspect Biol 2012 Jul 1;4(7):a006742. Epub 2012 Jul 1.

Department of Chemistry and Biochemistry, Howard Hughes Medical Institute, University of Colorado, Boulder, Colorado 80309-0215, USA.

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http://dx.doi.org/10.1101/cshperspect.a006742DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3385955PMC
July 2012

RNase P branches out from RNP to protein: organelle-triggered diversification?

Genes Dev 2012 May;26(10):1005-9

Howard Hughes Medical Institute, University of Colorado Biofrontiers Institute, Boulder Colorado 80309, USA.

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http://dx.doi.org/10.1101/gad.193581.112DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3360556PMC
May 2012

Mutually exclusive binding of telomerase RNA and DNA by Ku alters telomerase recruitment model.

Cell 2012 Mar 23;148(5):922-32. Epub 2012 Feb 23.

Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado Biofrontiers Institute, Boulder, CO 80309-0215, USA.

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http://dx.doi.org/10.1016/j.cell.2012.01.033DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3327133PMC
March 2012

DNA-induced dimerization of the single-stranded DNA binding telomeric protein Pot1 from Schizosaccharomyces pombe.

Nucleic Acids Res 2012 Jan 12;40(1):235-44. Epub 2011 Sep 12.

Department of Chemistry and Biochemistry, Howard Hughes Medical Institute, University of Colorado, Boulder, CO 80309-0215, USA.

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http://dx.doi.org/10.1093/nar/gkr721DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3245936PMC
January 2012

The RNA accordion model for template positioning by telomerase RNA during telomeric DNA synthesis.

Nat Struct Mol Biol 2011 Nov 20;18(12):1371-5. Epub 2011 Nov 20.

Department of Chemistry and Biochemistry, Howard Hughes Medical Institute, University of Colorado, Boulder, Colorado, USA.

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http://dx.doi.org/10.1038/nsmb.2174DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3230705PMC
November 2011

Multiple POT1-TPP1 proteins coat and compact long telomeric single-stranded DNA.

J Mol Biol 2011 Jul 9;410(1):10-7. Epub 2011 May 9.

Department of Pharmacology, Case Western Reserve University, Cleveland, OH 44106, USA.

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https://linkinghub.elsevier.com/retrieve/pii/S00222836110048
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http://dx.doi.org/10.1016/j.jmb.2011.04.049DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3157753PMC
July 2011

Ku can contribute to telomere lengthening in yeast at multiple positions in the telomerase RNP.

RNA 2011 Feb 21;17(2):298-311. Epub 2010 Dec 21.

Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218, USA.

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http://dx.doi.org/10.1261/rna.2483611DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3022279PMC
February 2011

Tetrahymena telomerase protein p65 induces conformational changes throughout telomerase RNA (TER) and rescues telomerase reverse transcriptase and TER assembly mutants.

Mol Cell Biol 2010 Oct 16;30(20):4965-76. Epub 2010 Aug 16.

Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309-0215, USA.

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http://dx.doi.org/10.1128/MCB.00827-10DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2950546PMC
October 2010

POT1-TPP1 enhances telomerase processivity by slowing primer dissociation and aiding translocation.

EMBO J 2010 Mar 21;29(5):924-33. Epub 2010 Jan 21.

Department of Chemistry and Biochemistry, Howard Hughes Medical Institute, University of Colorado-Boulder, 80309-0215, USA.

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http://dx.doi.org/10.1038/emboj.2009.409DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2837173PMC
March 2010

Engineering cis-telomerase RNAs that add telomeric repeats to themselves.

Proc Natl Acad Sci U S A 2010 Mar 1;107(11):4914-8. Epub 2010 Mar 1.

Department of Chemistry and Biochemistry, Howard Hughes Medical Institute, Campus Box 215, University of Colorado, Boulder, CO 80309-0215, USA.

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http://dx.doi.org/10.1073/pnas.0909366107DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2841905PMC
March 2010

Functional interaction between telomere protein TPP1 and telomerase.

Genes Dev 2010 Mar;24(6):613-22

Department of Chemistry and Biochemistry, Howard Hughes Medical Institute, University of Colorado, Boulder, 80309, USA.

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http://dx.doi.org/10.1101/gad.1881810DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2841338PMC
March 2010

How telomeric protein POT1 avoids RNA to achieve specificity for single-stranded DNA.

Proc Natl Acad Sci U S A 2010 Jan 22;107(2):651-6. Epub 2009 Dec 22.

Department of Chemistry and Biochemistry, Howard Hughes Medical Institute, University of Colorado, Boulder, CO 80309-0215, USA.

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http://dx.doi.org/10.1073/pnas.0911099107DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2796979PMC
January 2010

Inhibition of yeast telomerase action by the telomeric ssDNA-binding protein, Cdc13p.

Nucleic Acids Res 2009 Feb 29;37(2):354-67. Epub 2008 Nov 29.

Howard Hughes Medical Institute, University of Colorado, Boulder, CO 80309, USA.

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http://dx.doi.org/10.1093/nar/gkn830DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2632905PMC
February 2009

A natural ribozyme with 3',5' RNA ligase activity.

Nat Chem Biol 2009 Feb 4;5(2):97-9. Epub 2009 Jan 4.

Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, UCB 215, Boulder, Colorado 80309-0215, USA.

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http://dx.doi.org/10.1038/nchembio.136DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2897744PMC
February 2009

Crawling out of the RNA world.

Authors:
Thomas R Cech

Cell 2009 Feb;136(4):599-602

Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309, USA.

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http://dx.doi.org/10.1016/j.cell.2009.02.002DOI Listing
February 2009

Toward predicting self-splicing and protein-facilitated splicing of group I introns.

RNA 2008 Oct 3;14(10):2013-29. Epub 2008 Sep 3.

Howard Hughes Medical Institute, University of Colorado, Department of Chemistry and Biochemistry, Boulder, Colorado 80309-0215, USA.

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http://dx.doi.org/10.1261/rna.1027208DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2553746PMC
October 2008

Mutation in TERT separates processivity from anchor-site function.

Nat Struct Mol Biol 2008 Aug 20;15(8):870-2. Epub 2008 Jul 20.

Howard Hughes Medical Institute and Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309-0215, USA.

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http://dx.doi.org/10.1038/nsmb.1462DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2574958PMC
August 2008

Multiple yeast genes, including Paf1 complex genes, affect telomere length via telomerase RNA abundance.

Mol Cell Biol 2008 Jun 14;28(12):4152-61. Epub 2008 Apr 14.

Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309-0215, USA.

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http://dx.doi.org/10.1128/MCB.00512-08DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2423132PMC
June 2008

Triple-helix structure in telomerase RNA contributes to catalysis.

Nat Struct Mol Biol 2008 Jun 25;15(6):634-40. Epub 2008 May 25.

Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, Campus Box 215, University of Colorado, Boulder, Colorado 80309-0215, USA.

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http://dx.doi.org/10.1038/nsmb.1420DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2562722PMC
June 2008

On the occasion of the 25th anniversary of the discovery of catalytic RNA.

Authors:
Thomas R Cech

Biol Chem 2007 Jul;388(7):661-2

Howard Hughes Medical Institute, Chevy Chase, MD 20815, USA.

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http://dx.doi.org/10.1515/BC.2007.084DOI Listing
July 2007

Local RNA structural changes induced by crystallization are revealed by SHAPE.

RNA 2007 Apr 13;13(4):536-48. Epub 2007 Feb 13.

Department of Chemistry and Biochemistry, Howard Hughes Medical Institute, Univeristy of Colorado, Boulder, CO 80309-0215, USA.

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http://dx.doi.org/10.1261/rna.400207DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1831858PMC
April 2007

The POT1-TPP1 telomere complex is a telomerase processivity factor.

Nature 2007 Feb 21;445(7127):506-10. Epub 2007 Jan 21.

Department of Biological Chemistry, University of Michigan Medical School, MSRBIII 5301D, 1150 W. Medical Center Drive, Ann Arbor, Michigan 48109, USA.

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http://dx.doi.org/10.1038/nature05454DOI Listing
February 2007

Low abundance of telomerase in yeast: implications for telomerase haploinsufficiency.

RNA 2006 Sep 7;12(9):1721-37. Epub 2006 Aug 7.

Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309-0215, USA.

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http://dx.doi.org/10.1261/rna.134706DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1557690PMC
September 2006

Comparison of crystal structure interactions and thermodynamics for stabilizing mutations in the Tetrahymena ribozyme.

RNA 2006 Mar 23;12(3):387-95. Epub 2006 Jan 23.

Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309-0215, USA.

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http://dx.doi.org/10.1261/rna.2198206DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1383578PMC
March 2006

Crystal structure of the essential N-terminal domain of telomerase reverse transcriptase.

Nat Struct Mol Biol 2006 Mar 5;13(3):218-25. Epub 2006 Feb 5.

Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309-0215, USA.

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http://dx.doi.org/10.1038/nsmb1054DOI Listing
March 2006

Atomic level architecture of group I introns revealed.

Trends Biochem Sci 2006 Jan 13;31(1):41-51. Epub 2005 Dec 13.

Howard Hughes Medical Institute and Department of Chemistry and Biochemistry, University of Colorado, UCB 215, Boulder, CO 80309-0215, USA.

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http://dx.doi.org/10.1016/j.tibs.2005.11.008DOI Listing
January 2006

A miniature yeast telomerase RNA functions in vivo and reconstitutes activity in vitro.

Nat Struct Mol Biol 2005 Dec 20;12(12):1072-7. Epub 2005 Nov 20.

Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309-0215, USA.

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http://dx.doi.org/10.1038/nsmb1019DOI Listing
December 2005

Self-splicing and enzymatic activity of an intervening sequence RNA from Tetrahymena.

Authors:
Thomas R Cech

Biosci Rep 2004 Aug-Oct;24(4-5):362-85

Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309-0215, USA.

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http://dx.doi.org/10.1007/s10540-005-2738-3DOI Listing
November 2005

Global expression changes resulting from loss of telomeric DNA in fission yeast.

Genome Biol 2005 15;6(1):R1. Epub 2004 Dec 15.

Department of Chemistry and Biochemistry and Howard Hughes Medical Institute, University of Colorado, Boulder, CO 80309-0215, USA.

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http://dx.doi.org/10.1186/gb-2004-6-1-r1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC549061PMC
October 2005

POT1 stimulates RecQ helicases WRN and BLM to unwind telomeric DNA substrates.

J Biol Chem 2005 Sep 18;280(37):32069-80. Epub 2005 Jul 18.

Laboratory of Molecular Gerontology, National Institute on Aging, Baltimore, Maryland 21224, USA.

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http://dx.doi.org/10.1074/jbc.M505211200DOI Listing
September 2005

Fostering innovation and discovery in biomedical research.

Authors:
Thomas R Cech

JAMA 2005 Sep;294(11):1390-3

Howard Hughes Medical Institute, Chevy Chase, Md 20815, USA.

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http://dx.doi.org/10.1001/jama.294.11.1390DOI Listing
September 2005

Human POT1 disrupts telomeric G-quadruplexes allowing telomerase extension in vitro.

Proc Natl Acad Sci U S A 2005 Aug 25;102(31):10864-9. Epub 2005 Jul 25.

Howard Hughes Medical Institute and Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309-0215, USA.

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http://dx.doi.org/10.1073/pnas.0504744102DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1180509PMC
August 2005

Soluble domains of telomerase reverse transcriptase identified by high-throughput screening.

Protein Sci 2005 Aug;14(8):2051-8

Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309-0215, USA.

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http://dx.doi.org/10.1110/ps.051532105DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2279316PMC
August 2005

Switching human telomerase on and off with hPOT1 protein in vitro.

J Biol Chem 2005 May 24;280(21):20449-56. Epub 2005 Mar 24.

Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309-0215, USA.

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http://dx.doi.org/10.1074/jbc.M502212200DOI Listing
May 2005

Expression of a RecQ helicase homolog affects progression through crisis in fission yeast lacking telomerase.

J Biol Chem 2005 Feb 8;280(7):5249-57. Epub 2004 Dec 8.

Department of Chemistry and Biochemistry and Howard Hughes Medical Institute, University of Colorado, Boulder, CO 80309-0215, USA.

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http://dx.doi.org/10.1074/jbc.M412756200DOI Listing
February 2005

Structure of human POT1 bound to telomeric single-stranded DNA provides a model for chromosome end-protection.

Nat Struct Mol Biol 2004 Dec 21;11(12):1223-9. Epub 2004 Nov 21.

Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309-0215, USA.

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http://dx.doi.org/10.1038/nsmb867DOI Listing
December 2004

Nurturing interdisciplinary research.

Nat Struct Mol Biol 2004 Dec;11(12):1166-9

Howard Hughes Medical Institute, 4000 Jones Bridge Road, Chevy Chase, Maryland 20815, USA.

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http://dx.doi.org/10.1038/nsmb1204-1166DOI Listing
December 2004

Structure of the Tetrahymena ribozyme: base triple sandwich and metal ion at the active site.

Mol Cell 2004 Nov;16(3):351-62

Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309, USA.

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http://dx.doi.org/10.1016/j.molcel.2004.10.003DOI Listing
November 2004

The Euplotes telomerase subunit p43 stimulates enzymatic activity and processivity in vitro.

RNA 2004 Jul;10(7):1108-18

Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309-0215, USA.

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http://dx.doi.org/10.1261/rna.7400704DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1370601PMC
July 2004

Yeast telomerase RNA: a flexible scaffold for protein subunits.

Proc Natl Acad Sci U S A 2004 Jul 28;101(27):10024-9. Epub 2004 Jun 28.

Howard Hughes Medical Institute and Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309-0215, USA.

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http://www.pnas.org/cgi/doi/10.1073/pnas.0403641101
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http://dx.doi.org/10.1073/pnas.0403641101DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC454382PMC
July 2004

Managing your own lab.

Science 2004 Jun;304(5678):1717

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http://dx.doi.org/10.1126/science.304.5678.1717DOI Listing
June 2004

RNA finds a simpler way.

Authors:
Thomas R Cech

Nature 2004 Mar;428(6980):263-4

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http://dx.doi.org/10.1038/428263aDOI Listing
March 2004

Beginning to understand the end of the chromosome.

Authors:
Thomas R Cech

Cell 2004 Jan;116(2):273-9

Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309, USA.

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http://dx.doi.org/10.1016/s0092-8674(04)00038-8DOI Listing
January 2004

Tetrahymena telomerase is active as a monomer.

Mol Biol Cell 2003 Dec 17;14(12):4794-804. Epub 2003 Sep 17.

Children's Medical Research Institute, Westmead, New South Wales 2145, Australia.

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http://dx.doi.org/10.1091/mbc.e03-07-0474DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC284784PMC
December 2003

A template-proximal RNA paired element contributes to Saccharomyces cerevisiae telomerase activity.

RNA 2003 Nov;9(11):1323-32

Department of Chemistry and Biochemistry, and Howard Hughes Medical Institute, University of Colorado, Boulder, Colorado 80309-0215, USA.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1287054PMC
http://dx.doi.org/10.1261/rna.5570803DOI Listing
November 2003

DNA self-recognition in the structure of Pot1 bound to telomeric single-stranded DNA.

Nature 2003 Nov;426(6963):198-203

Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309-0215, USA.

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http://dx.doi.org/10.1038/nature02092DOI Listing
November 2003

Sequence-specific and 3'-end selective single-strand DNA binding by the Oxytricha nova telomere end binding protein alpha subunit.

Biochemistry 2003 Aug;42(31):9269-77

Department of Chemistry and Biochemistry and Howard Hughes Medical Institute, University of Colorado, Boulder, Colorado 80309-0215, USA.

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http://dx.doi.org/10.1021/bi0273718DOI Listing
August 2003

The Euplotes La motif protein p43 has properties of a telomerase-specific subunit.

Biochemistry 2003 May;42(19):5736-47

Department of Chemistry and Biochemistry and Howard Hughes Medical Institute, University of Colorado, Boulder, Colorado 80309-0215, USA.

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http://dx.doi.org/10.1021/bi034121yDOI Listing
May 2003

Human POT1 facilitates telomere elongation by telomerase.

Curr Biol 2003 May;13(11):942-6

Children's Medical Research Institute, 214 Hawkesbury Road, Westmead, NSW 2145, Australia.

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http://dx.doi.org/10.1016/s0960-9822(03)00339-7DOI Listing
May 2003