Publications by authors named "Thipsukon Boonyeun"

1 Publications

  • Page 1 of 1

Improvement of dioscorealide B production by elicitation in shoot cultures of Pierre ex Prain & Burkill.

Physiol Mol Biol Plants 2020 Mar 24;26(3):585-591. Epub 2020 Jan 24.

2Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Rangsit Campus, Pathumthani, 12120 Thailand.

Dioscorealide B is an important secondary metabolite isolated from Pierre ex Prain & Burkill. The effect on secondary metabolite content of different concentrations of two elicitors [jasmonic acid (JA) and salicylic acid (SA)], and of medium status and JA exposure period were investigated. In the JA and SA concentration experiment, 6-week-old shoots were cultured on MS medium supplemented with 8.87 µM BA (6-benzyladenine) in combination with 100-500 µM JA or 50-200 µM SA for 3 weeks. MS medium supplemented only with 8.87 µM BA was used as a control. The highest dioscorealide B content was recorded in the 100 µM JA shoots. To determine the optimal medium status and JA exposure period, shoots were cultured on solid and in liquid MS media supplemented with 8.87 µM BA and 100 µM JA for 2, 3, 4 and 5 weeks. No interaction was found between the medium status and the elicitor exposure period in the dioscorealide B production. Shoots cultured on the solid MS medium supplemented with 100 µM JA had a higher dioscorealide B content (0.57 ± 0.35% w/w) than those cultured in liquid medium (0.36 ± 0.40% w/w) and 5-week JA exposure produced the highest dioscorealide B content of 1.05 ± 0.15% (w/w).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12298-020-00762-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7078432PMC
March 2020