Publications by authors named "Thanet Pitakbut"

4 Publications

  • Page 1 of 1

Alpha-Glucosidase Inhibitory Assay-Screened Isolation and Molecular Docking Model from Active Compounds.

Molecules 2021 Oct 1;26(19). Epub 2021 Oct 1.

Department of Biochemical and Chemical Engineering, Technical University of Dortmund, 44227 Dortmund, Germany.

The aim of this research was to establish the constituents of as anti-diabetic agents. A phytochemistry analysis was conducted by chromatographic and spectroscopic techniques. The alpha-glucosidase inhibitory assay screening resulted in the isolation of eight known compounds of quercetin, quercitrin, luteolin, 5-deoxyluteolin, 4-methyl ether isoliquiritigenin, 3,2',4'-trihydroxy-4-methoxychalcone, stigmasterol and β-sitosterol. Ethanol leaf extracts showed potential effects, which led to a strong inhibitory activity of isolated quercetin at 138.95 µg/mL and 5.41 µg/mL of IC, respectively. The docking confirmed that flavonoids and chalcones had the same potential binding sites and responsibilities for their activity. This study was the first report of chemical constituents and its alpha-glucosidase inhibition.
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http://dx.doi.org/10.3390/molecules26195970DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8512368PMC
October 2021

Activity of THC, CBD, and CBN on Human ACE2 and SARS-CoV1/2 Main Protease to Understand Antiviral Defense Mechanism.

Planta Med 2021 Oct 12. Epub 2021 Oct 12.

Technical Biochemistry, Faculty of Biochemical and Chemical Engineering, TU Dortmund University, Dortmund, Germany.

THC, CBD, and CBN were reported as promising candidates against SARS-CoV2 infection, but the mechanism of action of these three cannabinoids is not understood. This study aims to determine the mechanism of action of THC, CBD, and CBN by selecting two essential targets that directly affect the coronavirus infections as viral main proteases and human angiotensin-converting enzyme2. Tested THC and CBD presented a dual-action action against both selected targets. Only CBD acted as a potent viral main protease inhibitor at the IC value of 1.86 ± 0.04 µM and exhibited only moderate activity against human angiotensin-converting enzyme2 at the IC value of 14.65 ± 0.47 µM. THC acted as a moderate inhibitor against both viral main protease and human angiotensin-converting enzymes2 at the IC value of 16.23 ± 1.71 µM and 11.47 ± 3.60 µM, respectively. Here, we discuss cannabinoid-associated antiviral activity mechanisms based on docking studies and receptor binding studies.
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http://dx.doi.org/10.1055/a-1581-3707DOI Listing
October 2021

In Vitro Production and Exudation of 20-Hydroxymaytenin from (Eckl. and Zeyh.) Loes. Cell Culture.

Plants (Basel) 2021 Jul 21;10(8). Epub 2021 Jul 21.

Technical Biochemistry, Department of Biochemical and Chemical Engineering, TU Dortmund University, 44227 Dortmund, Germany.

The metabolite 20-Hydroxymaytenin (20-HM) is a member of the quinone-methide pentacyclic triterpenoids (QMTs) group. This metabolite group is present only in Celastraceae plants, and it has shown various biological activities from antioxidant to anticancer properties. However, most QMTs metabolites including 20-HM cannot be synthesized in a laboratory. Therefore, we optimized a plant tissue culture protocol and examined the potential of (synonym. ) to produce 20-HM in an in vitro experiment. For the first time, we reported the optimum callus induction medium with a high percentage success rate of 82% from the combination of 1 mg/L indole-3-butyric acid and 5 mg/L naphthalene acetic acid. Later, our cell suspension culture cultivated in the optimum medium provided approximately 0.35 mg/g fresh weight of 20-HM. This concentration is roughly 87.5 times higher than a concentration of 20-HM presenting in (Celastraceae) leaves. In addition, we also found that 20-HM presented in a cultivation medium, suggesting that cells secreted 20-HM as an exudate in our experiment. Noticeably, 20-HM was missing when cf. occurred in the medium. These findings hint at an antifungal property of 20-HM.
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http://dx.doi.org/10.3390/plants10081493DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8398937PMC
July 2021

Determination of a-glucosidase inhibitory activity from selected Fabaceae plants.

Pak J Pharm Sci 2015 Sep;28(5):1679-83

Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla, Thailand.

Nineteen plants from Fabaceae family, which were used in Thai traditional medicine for treatment of diabetes, were determined of α-glucosidase inhibitory activity via enzymatic reaction. In this reaction, α-glucosidase was used as enzyme, which, reacted with the substrate, p-nitrophenol-D-glucopyranoside (pNPG). After that the product, p-nitro phenol (pNP) will be occurred and observed the yellow colour at 405 nm. In this study, acarbose was used as positive standard which, inhibited this enzyme with IC₅₀ as 331 ± 4.73 μg/ml. Caesalpinia pulcherrima leaves showed the highest activity with IC₅₀ as 436.97 ± 9.44 μg/ml. Furthermore, Bauhinia malabarica leaves presented moderately activity with IC₅₀ as 745.08 ± 11.15 μg/ml. However, the other plants showed mild to none activity of α-glucosidase inhibition. Accordingly, this study can support anti-diabetes of these plants in traditional medicine and it will be the database of the biological activity of Fabaceae plant.
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September 2015
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