Publications by authors named "Teresa Lucena"

31 Publications

Pseudidiomarina piscicola sp. nov., isolated from cultured European seabass, Dicenthrarchus labrax.

Arch Microbiol 2021 May 7;203(4):1293-1298. Epub 2020 Dec 7.

Departamento de Microbiología y Ecología and Colección Española de Cultivos Tipo (CECT), Universitat de València, València, Spain.

Strain CECT 9734, a Gram-negative, aerobic, chemoorganotrophic bacterium, motile by polar flagella, was isolated from cultured European seabass, Dicenthrarchus labrax, in Spain. It grows from 5 to 42 ºC, 6-9 pH and 1-12% total salinity. Major cellular fatty acids are C iso, summed feature 9 (C iso w9c/C 10-methyl) and C iso. The genome size is 2.5 Mbp and G + C content is 49.5 mol%. Comparative analysis of the 16S rRNA gene sequence shows that the strain is a member of Pseudidiomarina, with highest similarities with Pseudidiomarina halophila (97.0%) and Pseudidiomarina salinarum (96.9%). Phylogenomic tree based on UBCG program shows P. halophila as its closest relative. ANI and in-silico DDH with other Pseudidiomarina spp. are lower than 87 and 20%, respectively, suggesting that strain CECT 9734 represents a new species, for which we propose the name Pseudidiomarina piscicola sp. nov. and CECT 9734 (= LUBLD50 7a = LMG 31044) as type strain.
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http://dx.doi.org/10.1007/s00203-020-02131-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8055624PMC
May 2021

gcType: a high-quality type strain genome database for microbial phylogenetic and functional research.

Nucleic Acids Res 2021 01;49(D1):D694-D705

Microbial Resource and Big Data Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.

Taxonomic and functional research of microorganisms has increasingly relied upon genome-based data and methods. As the depository of the Global Catalogue of Microorganisms (GCM) 10K prokaryotic type strain sequencing project, Global Catalogue of Type Strain (gcType) has published 1049 type strain genomes sequenced by the GCM 10K project which are preserved in global culture collections with a valid published status. Additionally, the information provided through gcType includes >12 000 publicly available type strain genome sequences from GenBank incorporated using quality control criteria and standard data annotation pipelines to form a high-quality reference database. This database integrates type strain sequences with their phenotypic information to facilitate phenotypic and genotypic analyses. Multiple formats of cross-genome searches and interactive interfaces have allowed extensive exploration of the database's resources. In this study, we describe web-based data analysis pipelines for genomic analyses and genome-based taxonomy, which could serve as a one-stop platform for the identification of prokaryotic species. The number of type strain genomes that are published will continue to increase as the GCM 10K project increases its collaboration with culture collections worldwide. Data of this project is shared with the International Nucleotide Sequence Database Collaboration. Access to gcType is free at http://gctype.wdcm.org/.
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http://dx.doi.org/10.1093/nar/gkaa957DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7778895PMC
January 2021

Diversity and distribution of marine heterotrophic bacteria from a large culture collection.

BMC Microbiol 2020 07 13;20(1):207. Epub 2020 Jul 13.

Department of Marine Biology and Oceanography, Institut de Ciències del Mar (CSIC), 08003, Barcelona, Spain.

Background: Isolation of marine microorganisms is fundamental to gather information about their physiology, ecology and genomic content. To date, most of the bacterial isolation efforts have focused on the photic ocean leaving the deep ocean less explored. We have created a marine culture collection of heterotrophic bacteria (MARINHET) using a standard marine medium comprising a total of 1561 bacterial strains, and covering a variety of oceanographic regions from different seasons and years, from 2009 to 2015. Specifically, our marine collection contains isolates from both photic (817) and aphotic layers (744), including the mesopelagic (362) and the bathypelagic (382), from the North Western Mediterranean Sea, the North and South Atlantic Ocean, the Indian, the Pacific, and the Arctic Oceans. We described the taxonomy, the phylogenetic diversity and the biogeography of a fraction of the marine culturable microorganisms to enhance our knowledge about which heterotrophic marine isolates are recurrently retrieved across oceans and along different depths.

Results: The partial sequencing of the 16S rRNA gene of all isolates revealed that they mainly affiliate with the classes Alphaproteobacteria (35.9%), Gammaproteobacteria (38.6%), and phylum Bacteroidetes (16.5%). In addition, Alteromonas and Erythrobacter genera were found the most common heterotrophic bacteria in the ocean growing in solid agar medium. When comparing all photic, mesopelagic, and bathypelagic isolates sequences retrieved from different stations, 37% of them were 100% identical. This percentage increased up to 59% when mesopelagic and bathypelagic strains were grouped as the aphotic dataset and compared to the photic dataset of isolates, indicating the ubiquity of some bacterial isolates along different ocean depths. Finally, we isolated three strains that represent a new species, and the genome comparison and phenotypic characterization of two of these strains (ISS653 and ISS1889) concluded that they belong to a new species within the genus Mesonia.

Conclusions: Overall, this study highlights the relevance of culture-dependent studies, with focus on marine isolated bacteria from different oceanographic regions and depths, to provide a more comprehensive view of the culturable marine bacteria as part of the total marine microbial diversity.
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http://dx.doi.org/10.1186/s12866-020-01884-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7359222PMC
July 2020

Methylobacterium symbioticum sp. nov., a new species isolated from spores of Glomus iranicum var. tenuihypharum.

Curr Microbiol 2020 Sep 27;77(9):2031-2041. Epub 2020 Jun 27.

Symborg SL, Murcia, Spain.

Strain SB0023/3, isolated from spores of the arbuscular mycorrhizal fungus Glomus iranicum var. tenuihypharum, was analysed to determine whether it represents a new species. It was studied for its applicability in the field of agriculture to reduce the input of nitrogen fertilizers. Comparative analysis of the 16S rRNA gene sequence shows the strain to be affiliated to the genus Methylobacterium, the closest similarities (98.7%) being shared with Methylobacterium dankookense. Further phylogenomic analysis through Up-to-date Bacterial Core Gene (UBCG) confirmed Methylobacterium dankookense as its closest relative. Average Nucleotide Identity (ANI) and in silico DNA-DNA hybridization (DDH) were lower than 92% and 44%, respectively, of the values shown by its phylogenetic relatives. Its genome had an approximate length of 6.05 Mb and the G + C content of the genome was 70.1 mol%. The main cellular fatty acid was Summed Feature 8 (Cω7c and/or Cω6c). It is a Gram-staining-negative, pink-pigmented, strictly aerobic and facultative methylotroph; it grows at 28 ºC and can grow at up to 3% salinity in the presence of sodium chloride. All the data collected support the naming of a novel species to accommodate the strain SB0023/3, for which the name Methylobacterium symbioticum sp. nov. is proposed. The type strain is SB0023/3 (=CECT 9862 =PYCC 8351).
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http://dx.doi.org/10.1007/s00284-020-02101-4DOI Listing
September 2020

sp. nov., isolated from oceans during the oceans expedition, with a preference for mesopelagic waters.

Int J Syst Evol Microbiol 2020 Jul;70(7):4329-4338

Departamento de Microbiología y Ecología and Colección Española de Cultivos Tipo (CECT), Universitat de València, Valencia, Spain.

Strain ISS653, isolated from Atlantic seawater, is a yellow pigmented, non-motile, Gram-reaction-negative rod-shaped bacterium, strictly aerobic and chemoorganotrophic, slightly halophilic (1-15 % NaCl) and mesophilic (4-37 °C), oxidase- and catalase-positive and proteolytic. Its major cellular fatty acids are iso-C, iso-C 2-OH, and iso-C 3-OH; the major identified phospholipid is phosphatidylethanolamine and the major respiratory quinone is MK6. Genome size is 4.28 Mbp and DNA G+C content is 34.9 mol%. 16S rRNA gene sequence similarity places the strain among members of the family with the type strains of (93.2 %), (93.1 %) and (92.9 %) as closest relatives. Average amino acid identity (AAI) and average nucleotide identity (ANI) indices show highest values with (81 % AAI; 78.9 % ANI), (76 % AAI; 76.3 % ANI), (72 % AAI, 74.9 % ANI), (64 % AAI, 70.8 % ANI) and (68 % AAI; 72.2 % ANI). Phylogenomic analysis using the Up-to-date-Bacterial Core Gene set (UBCG) merges strain ISS653 in a clade with species of the genus . We conclude that strain ISS653 represents a novel species of the genus for which we propose the name sp. nov., and strain ISS653 (=CECT 9532=LMG 31236) as the type strain. A second strain of the species, ISS1889 (=CECT 30008) was isolated from Pacific Ocean seawater. Data obtained throughout the oceans expedition indicate that the species is more abundant in the mesopelagic dark ocean than in the photic layer and it is more frequent in the South Pacific, Indian and North Atlantic oceans.
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http://dx.doi.org/10.1099/ijsem.0.004296DOI Listing
July 2020

gen. nov., sp. nov., a novel member of the family .

Int J Syst Evol Microbiol 2020 Feb;70(2):1231-1239

Departamento de Microbiología y Ecología and Colección Española de Cultivos Tipo (CECT), Universitat de València, Valencia, Spain.

Strain ISS155, isolated from surface Mediterranean seawater, has cells that are Gram-reaction-negative, motile, strictly aerobic chemoorganotrophic, oxidase-positive, unable to reduce nitrate to nitrite, and able to grow with cellulose as the sole carbon and energy source. It is mesophilic, neutrophilic, slightly halophilic and has a requirement for sodium and magnesium ions. Its 16S rRNA gene sequence places the strain among members of , in the , with 017 as closest relative (94.3 % similarity). Its major cellular fatty acids are C, C and C; major phospholipids are phosphatidyl glycerol, phosphatidyl ethanolamine and an unidentified lipid, and the major respiratory quinone is Q8. The genome size is 6.09 Mbp and G+C content is 45.2 mol%. A phylogenomic analysis using UBCG merges strain ISS155 in a clade with , , and type strain genomes, all of them possessing a varied array of carbohydrate-active enzymes and the potential for polysaccharide degradation. Average amino acid identity indexes determined against available type strain genomes show that strain ISS155 is related to them by values lower than 60 %, with a maximum of 58 % to 017 and 57 % to T7902 and 2-40. These results, together with the low 16S rRNA gene sequence similarities and differences in phenotypic profiles, indicate that strain ISS155 represents a new genus and species in , for which we propose the name gen. nov., sp. nov., and strain ISS155 (=CECT 9533=LMG 31237) as the type strain.
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http://dx.doi.org/10.1099/ijsem.0.003906DOI Listing
February 2020

sp. nov., and reclassification of as comb. nov., and as comb. nov.

Int J Syst Evol Microbiol 2019 Nov;69(11):3405-3413

Departamento de Microbiología y Ecología and Colección Española de Cultivos Tipo (CECT), Universitat de València, Spain.

Strain CECT 7735, a marine Gram-reaction negative, aerobic, non-motile bacterium, was isolated from coastal seawater in Valencia, Spain. Strain CECT 7735 is chemoorganotrophic, mesophilic, slightly halophilic, grows at 15-28 °C but not at 4 or 37 °C, requires seawater for growth and grows up to 6 % salinity. The major cellular fatty acid is summed feature 8 (Cω7 and/or Cω6). The G+C content of the genome is 55.7 mol%. Comparative analysis of the 16S rRNA gene sequence shows the strain is affiliated to the family , in the class , with highest similarities to species (97.0-97.5 %), species (96.5-97.3 %) and (96.5 %). Further phylogenomic analysis through the up-to-date-bacterial core gene (UBCG) set showed to be its closest relative. Average nucleotide identity and DNA-DNA hybridization values are lower than 85 and 21 %, respectively, with its phylogenetic relatives, suggesting that strain CECT 7735 represents a new species. The average amino acid identity value was over 70 % with the genome of the type strain of and with all those of species. These values, together with UBCG set trees, suggest that the new species and belong to the same genus and that should be reclassified as a species. We conclude that strain CECT 7735 represents a new species in the genus , for which we propose the name sp. nov. In addition, is reclassified as comb. nov. From the same phylogenomic study, it can be concluded that should be reclassified in the genus as comb. nov.
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http://dx.doi.org/10.1099/ijsem.0.003629DOI Listing
November 2019

Ruegeria denitrificans sp. nov., a marine bacterium in the family Rhodobacteraceae with the potential ability for cyanophycin synthesis.

Int J Syst Evol Microbiol 2018 Aug 26;68(8):2515-2522. Epub 2018 Jun 26.

Departamento de Microbiología y Ecología and Colección Española de Cultivos Tipo (CECT), Universitat de València, Spain.

Strain CECT 5091, an aerobic, marine, Gram-reaction- and Gram-stain-negative, chemoheterotrophic bacterium was isolated from oysters harvested off the Spanish Mediterranean coast. Analysis of the 16S rRNA gene sequence placed the strain within the genus Ruegeria, in the family Rhodobacteraceae, with 16S rRNA gene similarities of 98.7, 98.7 and 98.4 % to Ruegeria conchae, Ruegeria atlanticaand Ruegeria arenilitoris, respectively. Average nucleotide identities (ANI) and in silico DNA-DNA hybridization (DDH) were determined, comparing the genome sequence of CECT 5091 with those of the type strains of 12 species of the genus Ruegeria: the values obtained were always below the thresholds (95-96 % ANI, 70 % in silico DDH) used to define genomic species, proving that CECT 5091 represents a novel species of the genus Ruegeria. The strain was slightly halophilic and mesophilic, with optimum growth at 26 °C, pH 7.0 and 3 % salinity, it required sodium and magnesium ions for growth and was able to reduce nitrate to dinitrogen. Carbon sources for growth include some carbohydrates (d-ribose, d-glucose, l-rhamnose, N-acetyl-d-glucosamine) and multiple organic acids and amino acids. The major cellular fatty acid was summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), representing 70 % of the total fatty acids. Carbon monoxide oxidation, cyanophycin synthetic ability and phosphatidylglycerol, diphosphatidylglycerol and phosphatidylcholine production are predicted from genome annotation, while bacteriochlorophyll a production was absent. The DNA G+C content of the genome was 56.7 mol%. We propose the name Ruegeriadenitrificans sp. nov. and strain CECT 5091 (=5OM10=LMG 29896) as the type strain for the novel species.
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http://dx.doi.org/10.1099/ijsem.0.002867DOI Listing
August 2018

Photobacterium malacitanum sp. nov., and Photobacterium andalusiense sp. nov., two new bacteria isolated from diseased farmed fish in Southern Spain.

Syst Appl Microbiol 2018 Sep 24;41(5):444-451. Epub 2018 May 24.

Universidad de Málaga, Departamento de Microbiología, Facultad de Ciencias, 29071 Málaga, Spain. Electronic address:

Three strains, H01100409B, H01100413B, and H27100402H, were isolated from several internal organs of diseased redbanded seabream (Pagrus auriga) reared in Andalusia (Southern Spain). All strains were studied by phenotypic, including chemotaxonomy, and genomic characteristics. Phylogenetic analysis based on concatenated sequences of six housekeeping genes (gyrB, ftsZ, topA, mreB, gapA, and 16S rRNA) supported the inclusion of the strains within the clade Phosphoreum of the genus Photobacterium, and two of the strains (H27100402H and H01100409B) formed a tight group separated from the closest species P. aquimaris. Genomic analyses, including average nucleotide identity (ANIb and ANIm) and DNA-DNA hybridization (DDH), clearly separated strains H27100402H and H01100409B from the other species within the clade Phosphoreum with values below the thresholds for species delineation. The chemotaxonomic features (including FAME analysis and MALDI-TOF-MS) of H27100402H and H01100409B strains confirmed their differentiation from the related taxa. The results demonstrated that strain H01100413B was classified as P. aquimaris and the strains H27100402H and H01100409B represented a new species each in the genus Photobacterium, for which we propose the names Photobacterium malacitanum sp. nov., type strain H27100402H (=CECT 9190=LMG 29992), and Photobacterium andalusiense sp. nov., type strain H01100409B (=CECT 9192=LMG 29994).
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http://dx.doi.org/10.1016/j.syapm.2018.04.005DOI Listing
September 2018

Comparative Genomics of Including the Description of sp. nov., and sp. nov.

Front Microbiol 2017 12;8:2645. Epub 2018 Jan 12.

Departamento de Microbiología and Colección Española de Cultivos Tipo, Universitat de València, Valencia, Spain.

A taxogenomic study was conducted to describe two new species and to analyze the internal consistency of the genus along with and . Strains CECT 5113, CECT 5114, CECT 5118, and CECT 5120 were isolated from coastal Mediterranean seawater, Spain. Cells were Gram-negative, non- motile coccobacilli, aerobic chemoorganotrophs, with an optimum temperature of 26°C and salinity of 3.5-5%. Major cellular fatty acids of strains CECT 5113 and CECT 5114 were C ω7c/ω6c and C 3OH, G+C content was 54.4-54.5 mol% and were able to utilize propionate, L-threonine, L- arginine, and L-aspartate as carbon sources. They exhibited 98.3% 16S rRNA gene sequence similarity, 75.0-75.1 ANIb and 19.5-20.9 digital DDH to type strain of their closest species, . Based on these data, strains CECT 5113 and CECT 5114 are recognized as a new species, for which the name is proposed, with strain CECT 5113 (=LMG 29900) as type strain. Strains CECT 5118 and CECT 5120 were found to constitute another new species, with major cellular fatty acids C ω/ω and C ω 11-methyl and a G+C content of 59.8 mol%; they were not able to utilize propionate, L-threonine, L- arginine or L-aspartate. Their closest species was , with values of 99.6% 16S rRNA gene sequence similarity, 79.1% ANIb and 23.2% digital DDH compared to the type strain, CECT 5383. The name is proposed for this second new species, with strain CECT 5118 (=LMG 29904) as type strain. To better determine the phylogenetic relationship of the two new species, we submitted 12 genomes representing species of , and , to a phylogenomic analysis based on 54 single protein-encoding genes (BCG54). The resulting phylogenomic tree did not agree with the current genera classification, as was divided in three clades, sensu stricto ( sp. nov., and ), plus the three spp (, and sp. SK013) and finally, plus sp. nov. remained apart from the two genera. Phenotypic inferences from explored genomes are presented.
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http://dx.doi.org/10.3389/fmicb.2017.02645DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5770400PMC
January 2018

Photobacterium toruni sp. nov., a bacterium isolated from diseased farmed fish.

Int J Syst Evol Microbiol 2017 Nov 21;67(11):4518-4525. Epub 2017 Sep 21.

Departamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, 29071 Málaga, Spain.

Three bacterial strains were isolated from liver and spleen of diseased farmed redbanded seabream (Pagrus auriga) in south-west Spain. Their partial 16S rRNA gene sequences clustered within those of the genus Photobacterium, showing high similarity (98.6-99.3 %) to the type strains of Photobacterium iliopiscarium, P. piscicola, P. kishitanii, P. aquimaris and P. phosphoreum. Multilocus sequence analysis using six housekeeping genes (gapA, topA, mreB, ftsZ, gyrB and 16S rRNA) confirmed the new strains as forming an independent branch with a bootstrap value of 100, likely to represent a novel species. To confirm this, we used whole genome sequencing and genomic analysis (ANIb, ANIm and in silico DNA-DNA hybridization) obtaining values well below the thresholds for species delineation. In addition, a phenotypic characterization was performed to support the description and differentiation of the novel strains from related taxa. Cells were Gram-stain-negative, motile bacilli, chemo-organotrophic and facultatively anaerobic. They fermented glucose, as well as galactose and d-mannose, without production of gas. Oxidase and catalase were positive. The predominant cellular fatty acids were C16 : 1ω7c/C16 : 1ω6c and C16  :  0. The predominant respiratory quinone (Q-8) and major polar lipids (phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol) were inferred from annotated genes in the genome of strain H01100410B, which had a G+C content of 38.6 mol%. The results obtained demonstrate that the three strains represent a novel species, for which the name Photobacterium toruni sp. nov. is proposed. The type strain is H01100410B (=CECT 9189=LMG 29991).
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http://dx.doi.org/10.1099/ijsem.0.002325DOI Listing
November 2017

Vibrio palustris sp. nov. and Vibrio spartinae sp. nov., two novel members of the Gazogenes clade, isolated from salt-marsh plants (Arthrocnemum macrostachyum and Spartina maritima).

Int J Syst Evol Microbiol 2017 Sep 31;67(9):3506-3512. Epub 2017 Aug 31.

Departamento de Microbiología y Ecología & Colección Española de Cultivos Tipo (CECT), Universitat de València, Spain.

Two bacterial strains, EAod9T and SMJ21T, isolated from salt-marsh plants, were determined to be related to species of the genus Vibriofrom from 16S rRNA sequence comparisons. Their closest phylogenetic relatives are members of the Gazogenes clade, Vibrio mangrovi and Vibrio rhizosphaerae , which show the greatest similarity to the SMJ21TrRNA sequence (97.3 and 97.1 %, respectively), while EAod9T had less than 97.0 % similarity to any other species of the genus Vibrio. Both strains share the basic characteristics of the genus Vibrio, as they are Gram-stain negative, motile, slightly halophilic, facultatively anaerobic bacteria. In addition, they are oxidase-negative and unable to grow on TCBS Agar; they grow between 15 to 26 °C, pH 6 to 8 and in up to 10 % (w/v) total salinity. They produce indol, are positive in the Voges-Proskauer test and are negative for arginine dihydrolase, lysine and ornithine decarboxylases. Strain SMJ21T is aerogenic and red-pigmented, due to prodigiosin production, while strain EAod9T ferments glucose without gas and is not pigmented. The major cellular fatty acids of both novel strains were C16 : 1ω7c/C16 : 1ω6c and C16 : 0. WGSobtained for both strains, along with the other five members of the clade, allowed the determination of ANI indexes and in silico estimations of DDH values, which confirmed that the two strains represent two novel species of the genus Vibrio: Vibriopalustris sp. nov. (with EAod9T=CECT 9027T=LMG 29724T as the proposed type strain) and Vibrio spartinae sp. nov. (with SMJ21T=CECT 9026T=LMG 29723T as the proposed type strain).
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http://dx.doi.org/10.1099/ijsem.0.002155DOI Listing
September 2017

Whole genome sequences reveal Vibrio hemicentroti Kim et al. 2013 as a later heterotypic synonym of Vibrio splendidus (Beijerinck 1900) Baumann et al. 1981.

Int J Syst Evol Microbiol 2017 Jun 30;67(6):1669-1671. Epub 2017 Jun 30.

Departamento de Microbiología y Ecología & Colección Española de Cultivos Tipo (CECT), Universitat de València, Valencia, Spain.

The synonymy between Vibrio hemicentrotiKim et al. 2013 and Vibrio splendidus(Beijerinck 1900) Baumann et al. 1981 was suggested after a recent multilocus sequence analysis of the Splendidus clade, which included the type strains of both species. To clarify their status, we have determined genomic indexes from whole genome sequences of strains V. hemicentroti CECT 8714T and V. splendidus NCCB 53037T. Average Nucleotide Identities of 96.0-96.7 % and an in silico DNA-DNA hybridization value of 70.2 %, as well as similarity levels of selected housekeeping gene sequences support the consideration of V.hemicentroti as a later heterotypic synonym of V. splendidus.
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http://dx.doi.org/10.1099/ijsem.0.001842DOI Listing
June 2017

Marinomonas blandensis sp. nov., a novel marine gammaproteobacterium.

Int J Syst Evol Microbiol 2016 Dec 9;66(12):5544-5549. Epub 2016 Oct 9.

Departamento de Microbiología y Ecología, Universitat de València, 46100 Burjassot (València), Spain.

A novel Gram-staining-negative, chemoorganotrophic, moderately halophilic, strictly aerobic bacterium, strain MED121T, was isolated from a seawater sample collected at the Blanes Bay Microbial Observatory in the north-western Mediterranean Sea. Analysis of its 16S rRNA gene sequence, retrieved from the whole-genome sequence, showed that this bacterium was most closely related to Marinomonas dokdonensis and other Marinomonas species (96.3 and 93.3-95.7 % sequence similarities, respectively), within the family Oceanospirillaceae. Strain MED121T was included into a whole-genome sequencing study and, subsequently, it was characterized using a polyphasic taxonomic approach. It was found to be oxidase and catalase positive, its cells are cocci to short rods, it does not ferment carbohydrates and does not reduce nitrate to nitrite or gas and it requires at least 2.5 % (w/v) marine salts and tolerates up to 7 % (w/v) salts. Its major cellular fatty acids in order of abundance are C16 : 1ω7c/C16 : 1ω6c, C18 : 1ω7c, C16 : 0 and C10 : 0 3-OH. Its genome had an approximate length of 5.1 million bases and a DNA G+C content equal to 40.9 mol%. Analysis of the annotated genes reveals the capacity for the synthesis of ubiquinone 8 (Q8) and the polar lipids phosphatidylglycerol and phosphatidylethanolamine, in agreement with other members of the genus. All the data collected supported the creation of a novel species to accommodate this bacterium, for which the name Marinomonas blandensis sp. nov. is proposed. The type strain is MED121T (=CECT 7076T=LMG 29722T).
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http://dx.doi.org/10.1099/ijsem.0.001554DOI Listing
December 2016

An MLSA approach for the taxonomic update of the Splendidus clade, a lineage containing several fish and shellfish pathogenic Vibrio spp.

Syst Appl Microbiol 2016 Sep 6;39(6):361-9. Epub 2016 May 6.

Departamento de Microbiología y Ecología, and Colección Española de Cultivos Tipo, CECT, Universitat de València, Spain. Electronic address:

A multilocus sequence analysis was undertaken in order to redefine the Splendidus clade of the genus Vibrio, a large group of species containing several pathogenic members that affect fish and shellfish, and are difficult to identify through both phenotypic and genotypic approaches. The study included analysis of partial sequences of recA, gyrB, mreB, rpoD and pyrH genes, as well as the 16S rRNA gene. Seventeen type strain species were included that were complemented with other reference strains and a collection of isolates tentatively identified as members of this clade, as well as a set of other Vibrio species. The clade was well defined and stable in all analyses, and was confirmed to contain V. celticus, V. atlanticus, V. artabrorum, V. toranzoniae and V. hemicentroti, in addition to the twelve previously recognized species. While some species were well-defined members (e.g. Vibrio cyclitrophicus, V. chagasii) others formed tight groups that were related by sequence similarities and lineage topology, which suggested a synonymy among their members, particularly the V. splendidus-V. hemicentroti pair. Most of the isolates were related to two major groups: the V. celticus-V. crassostreae-V. gigantis subclade that contained all isolates from oysters sampled in the cold season, and V. chagasii that included oyster isolates from warm months. This suggested a sharp seasonal occurrence for these species. None of the single genes were able to mimic the resolving power of the five-gene MLSA and none worked well for the identification of the whole group of species in the clade.
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http://dx.doi.org/10.1016/j.syapm.2016.03.010DOI Listing
September 2016

Grimontia celer sp. nov., from sea water.

Int J Syst Evol Microbiol 2016 Aug 28;66(8):2906-2909. Epub 2016 Apr 28.

Departamento de Microbiología y Ecología & Colección Española de Cultivos Tipo, Universitat de València, Spain.

Strain 96-237T, a Gram-reaction-negative, curved- to spiral-shaped motile bacterium, isolated from coastal marine water, was found to be related to species of the genus Grimontia by 16S rRNA gene sequence comparison, sharing 98.3 % similarity to Grimontia marina CECT 8713Tand 98.8 % to 'Grimontiaindica' AK16. Phenotypic analysis revealed that strain 96-237T is slightly halophilic, mesophilic and facultatively anaerobic, fermenting d-glucose, d-ribose, d-mannose, d-mannitol, maltose and sucrose. It was positive for oxidase and indole production and negative for arginine dihydrolase and lysine and ornithine decarboxylases. Its major fatty acids were C16 : 1ω7c/C16 : 1ω6c (SF3), C18 : 1ω7c and C16 : 0. Its DNA G+C content was 48.4 mol%. The strain was different at the species level from all other species of the genusGrimontia, with average nucleotide identity indices of 79.6 % to Grimontia. hollisae CECT 8713T, 87.8 % to G. marina CECT 5069T and 89.1 % to 'G. indica' AK16 genomes. Thus, the strain represents a novel species for which we propose the name Grimontia celer and 96-237T (=CECT 9029T =KCTC 42960T =LMG 29238T) as the type strain.
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http://dx.doi.org/10.1099/ijsem.0.001119DOI Listing
August 2016

Marinomonas spartinae sp. nov., a novel species with plant-beneficial properties.

Int J Syst Evol Microbiol 2016 Apr 27;66(4):1686-1691. Epub 2016 Jan 27.

Departamento de Microbiología y Ecología & Colección Española de Cultivos Tipo (CECT), Universitat de València, Spain.

Two strains of Gram-stain-negative, chemo-organotrophic, aerobic and halophilic gammaproteobacteria, isolated from within the stem and roots of Spartina maritima in salt marshes from the south Atlantic Spanish coast, were found to represent a novel species in the genus Marinomonas through phylogenetic analysis of their 16S rRNA genes and phenotypic characterization. 16S rRNA gene sequences of the two strains shared < 96.2% similarity with other Marinomonas species, with Marimonas alcarazii being the most similar in sequence. They required sodium ions for growth, were able to thrive at low (4 °C) temperatures and at salinities of 12-15%, were unable to hydrolyse any tested macromolecule except casein, and grew with different monosaccharides, disaccharides, sugar alcohols, organic acids and amino acids. The novel species differed from other Marinomonas species in the use of several sole carbon sources, its temperature and salinity ranges for growth, ion requirements and cellular fatty acid composition, which included C16:0, C16:1 and C18:1 as major components and C10:0 3-OH, C12:0 and C12:0 3-OH as minor components. The name Marinomonas spartinae sp. nov. is proposed, with SMJ19T (=CECT 8886T=KCTC 42958T) as the type strain.
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http://dx.doi.org/10.1099/ijsem.0.000929DOI Listing
April 2016

Multilocus Sequence Analysis of the redefined clade Scophthalmi in the genus Vibrio.

Syst Appl Microbiol 2015 May 25;38(3):169-75. Epub 2015 Mar 25.

Colección Española de Cultivos Tipo (CECT) and Departamento de Microbiología y Ecología, Universitat de València, Spain. Electronic address:

A Multilocus Sequence Analysis (MLSA) was performed on members of the Scophthalmi clade in the genus Vibrio, including type and reference strains of the species V. scophthalmi, V. ichthyoenteri, and 39 strains phenotypically identified as Vibrio ichthyoenteri-like, with the aim of better defining boundaries between these two closely related, fish-associated species. The type strain of V. ponticus, recently added to the clade Scophthalmi, was also included. The study was based on partial sequences of the protein-coding housekeeping genes rpoD, mreB, recA, ftsZ, and gyrB, and the 16S rRNA. While the 16S rRNA gene-based trees were unable to pull apart members of V. scophthalmi or V. ichthyoenteri, both the other individual gene trees and the trees obtained from the five-genes concatenated sequences were able to consistently differentiate four subclades within the main clade, corresponding to the bona fide V. scophthalmi, V. ichthyoenteri, and two small ones that may represent a new species each. The best genes to differentiate V. scophthalmi from V. ichthyoenteri were rpoD, recA, and mreB. Vibrio ponticus failed to associate to the clade in the MLSA and in most single gene trees for which it should not be considered part of it. In this study we also confirm using genomic indexes that V. ichthyoenteri and V. scophthalmi are two separate species.
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http://dx.doi.org/10.1016/j.syapm.2015.03.005DOI Listing
May 2015

Vibrio renipiscarius sp. nov., isolated from cultured gilthead sea bream (Sparus aurata).

Int J Syst Evol Microbiol 2015 Jun 20;65(Pt 6):1941-1945. Epub 2015 Mar 20.

Departamento de Microbiología y Ecología and Colección Española de Cultivos Tipo (CECT), Universidad de Valencia, Valencia, Spain.

Two strains of Gram-negative, facultatively anaerobic, slightly halophilic bacteria, isolated from healthy gilthead sea bream (Sparus aurata) cultured in Spanish Mediterranean fish farms, were different from their closest relatives, Vibrio scophthalmi and V. ichthyoenteri, by phenotypic, phylogenetic and genomic standards. The strains were negative for decarboxylase tests and lacked extracellular hydrolytic activities, but were able to ferment d-mannitol, sucrose, cellobiose and d-gluconate, among other carbohydrates. The major cellular fatty acids were C16:1 and C16:0, in agreement with other species of the genus Vibrio. Their 16S rRNA gene sequences were 98.4 and 97.2 % similar to those of the type strains of V. scophthalmi and V. ichthyoenteri, and the similarities using other housekeeping genes (ftsZ, rpoD, recA, mreB and gyrB) and indices of genomic resemblance (average nucleotide identity and estimated DNA-DNA hybridization) between the isolates and those type strains were clearly below intraspecific levels, supporting the recognition of the strains as members of a separate novel species. Thus, we propose the name Vibrio renipiscarius sp. nov., with DCR 1-4-2T ( = CECT 8603T = KCTC 42287T) as the type strain.
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http://dx.doi.org/10.1099/ijs.0.000200DOI Listing
June 2015

Draft Genome Sequences of Vibrio renopiscarius Strains CECT 8603T and CECT 8604, Two Marine Gammaproteobacteria Isolated from Cultured Gilthead Sea Bream (Sparus aurata).

Genome Announc 2015 Mar 12;3(2). Epub 2015 Mar 12.

Departamento de Microbiología y Ecología and Colección Española de Cultivos Tipo (CECT), Universitat de València, Valencia, Spain.

Vibrio renopiscarius DCR 1-4-2(T) (CECT 8603(T)) and DCR 1-4-12 (CECT 8604) were isolated from healthy gilthead sea bream (Sparus aurata) from Mediterranean fish farms (Castellón, Spain). Their draft genome sequences (30 and 44 contigs, respectively) have 4.3 Mbp and a G+C content of 45.2 mol% and contain almost 3,700 protein-encoding genes.
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http://dx.doi.org/10.1128/genomeA.00099-15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4357758PMC
March 2015

Multilocus sequence analysis of putative Vibrio mediterranei strains and description of Vibrio thalassae sp. nov.

Syst Appl Microbiol 2014 Jul 27;37(5):320-8. Epub 2014 May 27.

Departamento de Microbiología y Ecología and Colección Española de Cultivos Tipo (CECT), Universidad de Valencia, Spain. Electronic address:

A multilocus sequence analysis based on partial gyrB, mreB, rpoD and pyrH genes was undertaken with 61 putative Vibrio mediterranei/V. shilonii strains from different hosts (mussels, oysters, clams, coral, fish and plankton) or habitat (seawater and sediment) and geographical origins (Mediterranean, Atlantic and Pacific). A consistent grouping was obtained with individual and concatenated gene sequences, and the clade, comprising 54 strains, was split into three subclades by all methods: subclade A (40 strains, including AK1, the former type strain of Vibrio shilonii), subclade B (8 strains) corresponding to the species V. mediterranei, and subclade C (six strains) representing a new species, V. thalassae sp. nov., with strain MD16(T) (=CECT 8203(T)=KCTC 32373(T)) as the proposed type strain. Average nucleotide identity (ANI) values, determined as a measure of genomic similarity, confirmed these assignments, and supported that strains in subclade C were a different species from V. mediterranei, with ANIb and ANIm figures lower than 90.0%. The synonymy of V. shilonii and V. mediterranei was also stressed by both MLSA and ANI determinations (97.0% between both type strains). No connection was found between geographic origin or sample type and MLSA grouping.
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http://dx.doi.org/10.1016/j.syapm.2014.05.005DOI Listing
July 2014

Roseovarius albus sp. nov., a new Alphaproteobacterium isolated from the Mediterranean Sea.

Antonie Van Leeuwenhoek 2014 Apr 1;105(4):671-8. Epub 2014 Feb 1.

Colección Española de Cultivos Tipo (CECT) and Departamento de Microbiología y Ecología, Universitat de València, Valencia, Spain,

Strain 4SM10(T), an aerobic marine, Gram-negative, heterotrophic and non pigmented bacterium isolated from seawater from Vinaroz in Castellón, Spain, was characterized using a polyphasic approach. Analysis of the 16S rRNA gene sequence placed the strain within the Roseobacter clade in the family Rhodobacteraceae. Phylogenetic analyses also showed that strain 4SM10(T) forms a stable clade with species of the genus Roseovarius, being related to Roseovarius nubinhibens ISM(T) and Roseovarius aestuarii SMK-122(T) at 97.5 and 97.4 % 16S rRNA sequence similarity, respectively. Average Nucleotide Identity (ANI) values, determined as a measure of overall genomic resemblance, confirmed that strain 4SM10(T) does not belong to the same species as R. aestuarii CECT 7745(T) and Roseovarius nubinhibens CECT 7750(T) displaying ANI values well below the 95 % boundary for genomic species. Strain 4SM10(T) requires Na(+) plus a divalent cation (either Mg(2+) or Ca(2+)) to grow, reduces nitrate to nitrite and uses a large number of amino acids and organic acids (but no carbohydrates) as sole carbon sources. Enzymatic activities displayed in API ZYM tests are alkaline phosphatase, leucine arylamidase and acid phosphatase. The major cellular fatty acids were identified as C18:1 ω7c and/or C18:1 ω6c (67.1 %). The DNA G+C content was determined to be 54.27 mol%. Based on the genotypic and phenotypic data obtained, the name Roseovarius albus sp. nov. is proposed for this novel taxon, with the type strain 4SM10(T) (=CECT 7450(T) = KCTC 22653(T)).
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http://dx.doi.org/10.1007/s10482-014-0121-8DOI Listing
April 2014

Description of Tropicibacter mediterraneus sp. nov. and Tropicibacter litoreus sp. nov.

Syst Appl Microbiol 2013 Jul 23;36(5):325-9. Epub 2013 May 23.

Colección Española de Cultivos Tipo CECT and Departamento de Microbiología y Ecología, Universidad de Valencia, Spain.

Four strains (M15∅_3, M17(T), M49 and R37(T)) were isolated from Mediterranean seawater at Malvarrosa beach, Valencia, Spain. Together with an older preserved isolate (strain 2OM6) from cultured oysters at Vinaroz, Castellón, Spain, the strains were thoroughly characterized in a polyphasic study and were placed phylogenetically within the Roseobacter clade in the family Rhodobacteraceae. Highest 16S rRNA sequence similarities of the five strains to the types of any established species corresponded to Tropicibacter multivorans (95.8-96.4%), Phaeobacter inhibens (95.9-96.3%) and Phaeobacter gallaeciensis (95.9-96.2%). On the other hand, whole genome (ANI) and protein fingerprinting (MALDI-TOF) data proved: (i) non clonality among the strains, and (ii) the existence of two genospecies, one consisting of strains M15∅_3, M17(T), M49 and 2OM6 and another one consisting of strain R37(T). Phenotypic traits determined allow differentiating both genospecies from each other and from closely related taxa. In view of all data collected we propose to accommodate these isolates in two species as members of the genus Tropicibacter, Tropicibacter mediterraneus sp. nov. (type strain M17(T)=CECT 7615(T)=KCTC 23058(T)) and Tropicibacter litoreus sp. nov. (type strain R37(T)=CECT 7639(T)=KCTC 23353(T)).
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http://dx.doi.org/10.1016/j.syapm.2013.04.001DOI Listing
July 2013

Marinifilum flexuosum sp. nov., a new Bacteroidetes isolated from coastal Mediterranean Sea water and emended description of the genus Marinifilum Na et al., 2009.

Syst Appl Microbiol 2013 May 18;36(3):155-9. Epub 2013 Jan 18.

Departamento de Microbiología y Ecología and Colección Española de Cultivos Tipo (CECT), Universidad de Valencia, Spain.

A facultatively anaerobe, moderately halophilic, Gram-negative, filamentous, non motile and unpigmented bacterium, designated M30(T), was isolated from coastal Mediterranean Sea water in Valencia, Spain. Phylogenetic analysis based on 16S rRNA sequences placed this strain in the phylum "Bacteroidetes" with Marinifilum fragile JC2469(T) as its closest relative with 97% sequence similarity. Average nucleotide identity (ANI) values between both strains were far below the 95% threshold value for species delineation (about 89% using BLAST and about 90% using MUMmer). A comprehensive polyphasic study, including morphological, biochemical, physiological, chemotaxonomic and phylogenetic data, confirmed the independent species status of strain M30(T) within the genus Marinifilum, for which the name Marinifilum flexuosum sp. nov. is proposed. The type strain of Marinifilum flexuosum is M30(T) (=CECT 7448(T)=DSM 21950(T)).
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http://dx.doi.org/10.1016/j.syapm.2012.12.003DOI Listing
May 2013

Vibrio aestivus sp. nov. and Vibrio quintilis sp. nov., related to Marisflavi and Gazogenes clades, respectively.

Syst Appl Microbiol 2012 Oct 8;35(7):427-31. Epub 2012 Sep 8.

Colección Española de Cultivos Tipo (CECT) and Departamento de Microbiología y Ecología, Universitat de València, Spain.

Two new Vibrio species, Vibrio aestivus and Vibrio quintilis, are described after a polyphasic characterization of strains M22(T), M61 and M62(T), isolated from seawater collected off a beach on the East coast of Spain (Valencia). All three strains are Gram negative, mesophilic, slightly halophilic, fermentative rods. V. aestivus (M22(T)=CECT 7558(T)=CAIM 1861(T)=KCTC 23860(T) and M61=CECT 7559=CAIM 1862=KCTC 23861) is oxidase positive, reduces nitrates to nitrites, is negative for Voges Proskauer, arginine dihydrolase and indole and non hydrolytic on most substrates tested. The 16S rRNA gene sequences of M22(T) and M61 are most similar to Vibrio marisflavi (97.1-97.2%) but phylogenetic analysis using NJ, MP and ML methods display Vibrio stylophorae (96.2% similarity) as sibling species. The three species form a deep clade in the genus Vibrio. Average Nucleotide Identity (ANI) values, determined as a measure of overall genomic resemblance, confirmed that strains M22(T) and M61 are members of the same species, different to V. marisflavi CECT 7928(T). V. quintilis (M62(T)=CECT 7734(T)=CAIM 1863(T)=KCTC 23833(T)) is aerogenic, arginine dihydrolase and Voges Proskauer positive, oxidase negative and unable to reduce nitrate, traits shared by most species in the Gazogenes clade. It is unpigmented and does not grow on TCBS Agar. 16S rRNA gene similarities to its nearest species, Vibrio aerogenes and Vibrio mangrovi, are 97.6% and 96.0% respectively. Strain M62(T) and V. aerogenes CECT 7868(T) display ANI values well below the 95% boundary for genomic species.
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http://dx.doi.org/10.1016/j.syapm.2012.08.002DOI Listing
October 2012

Actibacterium mucosum gen. nov., sp. nov., a marine alphaproteobacterium from Mediterranean seawater.

Int J Syst Evol Microbiol 2012 Dec 6;62(Pt 12):2858-2864. Epub 2012 Jan 6.

Departamento de Microbiología y Ecología and Colección Española de Cultivos Tipo (CECT), Universidad de Valencia, Valencia, Spain.

Strain R46(T), a marine alphaproteobacterium, was isolated from Mediterranean seawater at Malvarrosa beach, Valencia, Spain. It is an aerobic chemo-organotrophic, mesophilic and slightly halophilic organism, with complex ionic requirements. Phylogenetic analyses based on the 16S rRNA and gyrB gene sequences showed that strain R46(T) formed a separate branch within the family Rhodobacteraceae, bearing similarities below 94.7 and 80.3%, respectively, to any other recognized species. It contained Q10 as the predominant isoprenoid quinone and C(18:1)ω7c/C(18:1)ω6c as the major cellular fatty acid. Phosphatidylglycerol was the only identified polar lipid, although other lipids were also detected. The DNA G+C content of the genomic DNA was 61.3 mol%. On the basis of extensive phenotypic and phylogenetic comparative analysis, it is concluded that the strain represents a novel genus and species, for which the name Actibacterium mucosum gen. nov., sp. nov. is proposed. The type strain of the type species is Actibacterium mucosum R46(T) ( = CECT 7668(T) = KCTC 23349(T)).
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http://dx.doi.org/10.1099/ijs.0.038026-0DOI Listing
December 2012

Tropicibacter multivorans sp. nov., an aerobic alphaproteobacterium isolated from surface seawater.

Int J Syst Evol Microbiol 2012 Apr 20;62(Pt 4):844-848. Epub 2011 May 20.

Departamento de Microbiología y Ecología, Colección Española de Cultivos Tipo (CECT), Universidad de Valencia, Valencia, Spain.

Strain MD5T, an aerobic marine alphaproteobacterium, was isolated from Mediterranean seawater at Malvarrosa beach, Valencia, Spain. The strain was characterized in a polyphasic study and was placed phylogenetically within the Roseobacter clade in the family Rhodobacteraceae. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain MD5T is related to Tropicibacter naphthalenivorans C02T, Phaeobacter inhibens T5T, P. gallaeciensis BS107T and P. daeponensis TF-218T, with 96.9, 96.2, 96.1 and 96.1 % sequence similarity, respectively. Phylogenetic analyses also showed that strain MD5T forms a stable clade only with T. naphthalenivorans C02T. Strain MD5T requires Na+ plus a divalent cation (either Mg2+ or Ca2+) to grow, does not reduce nitrate to nitrite and uses a large number of carbohydrates as sole carbon sources. It is positive for β-galactosidase and urease activities and aesculin hydrolysis. Enzyme activities displayed in the API ZYM strip were alkaline phosphatase, leucine arylamidase, acid phosphatase and α-glucosidase. Major cellular fatty acids were summed feature 8 (C18:1ω7c and/or C18:1ω6c; 70.9 %) and C16:0 (8.2 %). The results of physiological and biochemical tests allowed clear phenotypic differentiation of this isolate from the only described species of the genus Tropicibacter. It is evident from the genotypic and phenotypic data obtained that the strain should be classified in a novel species in the genus Tropicibacter. The name Tropicibacter multivorans sp. nov. is proposed, with the type strain MD5T (=CECT 7557T=KCTC 23350T).
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http://dx.doi.org/10.1099/ijs.0.030973-0DOI Listing
April 2012

Pseudomonas litoralis sp. nov., isolated from Mediterranean seawater.

Int J Syst Evol Microbiol 2012 Feb 1;62(Pt 2):438-444. Epub 2011 Apr 1.

Departamento de Microbiología y Ecología, Colección Española de Cultivos Tipo (CECT), Universidad de Valencia, 46980 Paterna (Valencia), Spain.

Strains 2SM5(T) and 2SM6, two strictly aerobic chemo-organotrophic gammaproteobacteria, were isolated from Mediterranean seawater off the coast of Vinaroz, Castellón, Spain, in February, 1990. They were extensively characterized by a polyphasic study that placed them in the genus Pseudomonas. Phylogenetic analysis of 16S rRNA gene sequences showed that both strains shared 100 % sequence similarity and were closely related to members of the Pseudomonas pertucinogena clade, with less than 97.3 % similarity to strains of established species; Pseudomonas xiamenensis was the closest relative. Analysis of sequences of three housekeeping genes, rpoB, rpoD and gyrB, further confirmed the phylogenetic assignment of the Mediterranean isolates. Chemotaxonomic traits such as quinone and polar lipid composition also corroborated the placement of strains 2SM5(T) and 2SM6 in the gammaproteobacteria. Other phenotypic traits, including fatty acid composition, enabled clear differentiation of both isolates from other species of Pseudomonas. We therefore conclude that strains 2SM5(T) and 2SM6 represent a novel species of Pseudomonas, for which the name Pseudomonas litoralis is proposed; the type strain is 2SM5(T) ( = CECT 7670(T) = KCTC 23093(T)).
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http://dx.doi.org/10.1099/ijs.0.029447-0DOI Listing
February 2012

Photobacterium aphoticum sp. nov., isolated from coastal water.

Int J Syst Evol Microbiol 2011 Jul 30;61(Pt 7):1579-1584. Epub 2010 Jul 30.

Departamento de Microbiología y Ecología, Colección Española de Cultivos Tipo (CECT), Universidad de Valencia, Spain.

A facultatively anaerobic marine gammaproteobacterium, designated strain M46(T), was isolated from Mediterranean seawater at Malvarrosa beach, Valencia, Spain. The strain was characterized by using a polyphasic approach and was found to be situated within the genus Photobacterium in the family Vibrionaceae. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M46(T) was closely related to P. rosenbergii CECT 7644(T), P. halotolerans CECT 5860(T) and P. ganghwense CECT 7641(T), showing sequence similarities of 96.8, 96.4 and 96.2 %, respectively. According to the results of phylogenetic analyses based on recA and gyrB gene sequences, the most closely related taxon was P. ganghwense CECT 7641(T) with 87.4 and 85.0 % sequence similarity, respectively. Regardless of the gene used in phylogenetic analysis, strain M46(T) always formed a separate and stable clade containing these three species of the genus Photobacterium. Strain M46(T) was not luminescent and produced a diffusible brown pigment. It required NaCl to grow, reduced nitrate to nitrite and oxidized a small number of substrates in Biolog GN plates. Strain M46(T) was positive for arginine dihydrolase (ADH), β-galactosidase, aesculin hydrolysis and DNase activity. In API ZYM tests, the novel strain was positive for alkaline phosphatase, leucine arylamidase and acidic phosphatase activities. The major cellular fatty acids were unsaturated C(18) and C(16), as in other members of the genus Photobacterium, but their relative amounts and the presence or absence of other fatty acids differentiated strain M46(T) from its closest relatives. Based on the results of this polyphasic taxonomic study, strain M46(T) represents a novel species of the genus Photobacterium, for which the name Photobacterium aphoticum is proposed. The type strain is M46(T) ( = CECT 7614(T)  = KCTC 23057(T)).
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http://dx.doi.org/10.1099/ijs.0.025171-0DOI Listing
July 2011

Euzebyella saccharophila gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae.

Int J Syst Evol Microbiol 2010 Dec 15;60(Pt 12):2871-2876. Epub 2010 Jan 15.

Colección Española de Cultivos Tipo (CECT) y Departamento de Microbiología y Ecología, Universidad de Valencia, Spain.

Strain 7SM30(T)(,) an aerobic marine, Gram-negative, heterotrophic and yellow- to orange-pigmented bacterium isolated from seawater from Castellón, Spain, was characterized using a polyphasic approach. Analysis of the 16S rRNA gene sequence showed that the isolate represented a novel lineage within the family Flavobacteriaceae. The most closely related genera were Pseudozobellia, Zobellia and Kriegella. Cells of strain 7SM30(T) were non-motile rods that required sea salts for growth, used a wide variety of carbohydrates as sole carbon and energy sources and, unlike species of the genera Pseudozobellia and Zobellia, did not possess flexirubin-type pigment or hydrolyse agar. Strain 7SM30(T) contained MK6 as the sole respiratory quinone. Phosphatidylethanolamine (PE) was the only identifiable polar lipid, although other lipids were also detected. The predominant cellular fatty acids were saturated C(15) and monounsaturated C(15). The DNA G+C content was around 40 mol%. On the basis of extensive phenotypic and phylogenetic comparative analysis, it is concluded that the new strain represents a novel genus and species, for which the name Euzebyella saccharophila gen. nov., sp. nov., is proposed. The type strain of the type species is 7SM30(T) (=CECT 7477(T)=KCTC 22655(T)).
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http://dx.doi.org/10.1099/ijs.0.020875-0DOI Listing
December 2010