Publications by authors named "Teresa Garcia"

132 Publications

Aesthetic forehead reduction in female patients: Surgical details and analysis of outcome.

J Plast Reconstr Aesthet Surg 2021 Jun 18. Epub 2021 Jun 18.

Unit of Plastic Surgery, Hospital la Luz, Madrid, Spain.

Introduction: Primary aesthetic hairline lowering, also known as forehead reduction, is a relatively unexplored procedure, and the perceived satisfaction with outcome has not been previously discussed in scientific literature. The objectives of this study were to review the surgical technique and to analyze outcome assessing objective reduction, improvement of facial balance, patient satisfaction, and complications.

Patients And Methods: This was a prospective cohort study of patients seeking aesthetic forehead reduction since 2010. Inclusion criteria were as follows: women with a high but stable hairline. We excluded smokers, those seeking combined surgery, and those who had history of scalp surgery or hair loss. All patients were operated following the same protocol. Forehead reduction was measured comparing the pre- and postoperative distance from the glabella to the trichion. Improvement in facial balance was assessed comparing the pre- and postoperative quotient between the upper and middle thirds of the face. The forehead FACE-Q tool and the Vancouver Scar Scale were used to assess patient satisfaction. All complications were registered with a minimum follow-up of 6 months.

Results: Twenty-six women aged 16 to 56 years were included. The average reduction was 2.03 cm. The facial balance quotient improved from 1:1.44 preoperatively to 1:1.05 postoperatively (p < 0.01). Both the forehead FACE-Q and the Vancouver Scar Scale showed significantly positive results. Complications included transient scalp numbness in 23 subjects, small areas of scar widening in 3 patients, and minor seroma in 1 patient.

Conclusion: With meticulous execution, aesthetic forehead reduction in female patients is effective and safe, resulting in high patient satisfaction. The FACE-Q is owned by the Memorial Sloan Kettering Cancer Center (MSKCC) and was used with permission for this research.
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http://dx.doi.org/10.1016/j.bjps.2021.06.002DOI Listing
June 2021

Ischemic Cerebellar Stroke in a 4-Year-Old Boy After Chickenpox: An Atypical Vascular Involvement.

J Stroke Cerebrovasc Dis 2021 Jun 1;30(6):105719. Epub 2021 Apr 1.

Child Development Centre, Pediatric Hospital, Centro Hospitalar e Universitário de Coimbra, Coimbra, Portugal. Electronic address:

Cerebrovascular events in pediatric population are very rare. Up to 30% may result from varicella zoster (VZV) arteriopathy, usually as a delayed complication of varicella primary infection. The most typical pattern includes involvement of anterior brain circulation arteries, probably by VZV migration from the trigeminal ganglia. Strokes related with VZV usually have a good prognosis, but risk of recurrence is greater when compared to other stroke etiologies in this age group. We report the case of a 4-year-old boy, immunocompetent, who presented a basilar artery stenosis and a cerebellar stroke, an extremely rare presentation of VZV arteriopathy. The investigation workup and treatment are detailed, as the clinical and imaging follow-up after one year.
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http://dx.doi.org/10.1016/j.jstrokecerebrovasdis.2021.105719DOI Listing
June 2021

Life-Threatening Acute Angioedema, a Rare Complication After Secondary Blepharoplasty.

Aesthet Surg J 2021 Mar 31. Epub 2021 Mar 31.

Plastic Surgery Unit, Hospital Universitario La Luz, Madrid, Spain.

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http://dx.doi.org/10.1093/asj/sjab093DOI Listing
March 2021

Survey of Commercial Food Products for Detection of Walnut () by Two ELISA Methods and Real Time PCR.

Foods 2021 Feb 17;10(2). Epub 2021 Feb 17.

Departamento de Nutrición y Ciencia de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain.

Labeling of food allergens in accordance with legal regulations is important to protect the health of allergic consumers. The requirements for detecting allergens in foods involve adequate specificity and sensitivity to identify very small amounts of the target allergens in complex food matrices and processed foods. In this work, one hundred commercial samples were analyzed for walnut detection using three different methods: a sandwich enzyme-linked immunosorbent assay (ELISA) kit based on polyclonal antibodies, a direct ELISA using a recombinant multimeric scFv, and a real time PCR. The most sensitive method was real time PCR followed by sandwich ELISA kit and multimeric scFv ELISA. There was agreement between the three methods for walnut detection in commercial products, except for some heat-treated samples or those that contained pecan. The walnut ELISA kit was less affected by sample processing than was the multimeric scFv ELISA, but there was cross-reactivity with pecan, producing some false positives that must be confirmed by real time PCR. According to the results obtained, 7.0 to 12.6% of samples (depending on the analytical method) contained walnut but did not declare it, confirming there is a risk for allergic consumers. Moreover, there was one sample (3.7%) labelled as containing walnut but that tested negative for this tree nut. Genetic and immunoenzymatic techniques offer complementary approaches to develop a reliable verification for walnut allergen labeling.
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http://dx.doi.org/10.3390/foods10020440DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7922217PMC
February 2021

Impact of Obesity on Quality of Life, Psychological Distress, and Coping on Patients with Colon Cancer.

Oncologist 2021 05 13;26(5):e874-e882. Epub 2021 Feb 13.

Department of Clinical Psychology and Psychobiology, Faculty of Psychology, University of Barcelona, Barcelona, University of País Vasco, País Vasco, Spain.

Background: Despite the causal relationship between obesity and colon cancer being firmly established, the effect of obesity on the course of cancer calls for further elucidation. The objective of this study was to assess differences in clinical-pathological and psychosocial variables between obese and nonobese individuals with colon cancer.

Materials And Methods: This was a prospective, multicentric, observational study conducted from 2015-2018. The sample comprised patients with stage II-III, resected colon cancer about to initiate adjuvant chemotherapy with fluoropyrimidine in monotherapy or associated with oxaliplatin and grouped into nonobese (body mass index <30 kg/m ) or obese (≥30 kg/m ). Subjects completed questionnaires appraising quality of life (European Organization for Research and Treatment of Cancer Quality of Life Core questionnaire), coping (Mini-Mental Adjustment to Cancer), psychological distress (Brief Symptom Inventory 18), perceived social support (Multidimensional Scale of Perceived Social Support), personality (Big Five Inventory 10), and pain (Brief Pain Inventory). Toxicity, chemotherapy compliance, 12-month recurrence, and mortality rate data were recorded.

Results: Seventy-nine of the 402 individuals recruited (19.7%) were obese. Obese subjects exhibited more comorbidities (≥2 comorbidities, 46.8% vs. 30.3%, p = .001) and expressed feeling slightly more postoperative pain (small size-effect). There was more depression, greater helplessness, less perceived social support from friends, and greater extraversion among the obese versus nonobese subjects (all p < .04). The nonobese group treated with fluoropyrimidine and oxaliplatin suffered more grade 3-4 hematological toxicity (p = .035), whereas the obese had higher rates of treatment withdrawal (17.7% vs. 7.7%, p = .033) and more recurrences (10.1% vs. 3.7%, p = .025). No differences in sociodemographic, quality of life, or 12-month survival variables were detected.

Conclusion: Obesity appears to affect how people confront cancer, as well as their tolerance to oncological treatment and relapse.

Implications For Practice: Obesity is a causal factor and affects prognosis in colorectal cancer. Obese patients displayed more comorbidities, more pain after cancer surgery, worse coping, and more depression and perceived less social support than nonobese patients. Severe hematological toxicity was more frequent among nonobese patients, whereas rates of withdrawal from adjuvant chemotherapy were higher in the obese cohort, and during follow-up, obese patients presented greater 12-month recurrence rates. With the growing and maintained increase of obesity and the cancers associated with it, including colorectal cancer, the approach to these more fragile cases that have a worse prognosis must be adapted to improve outcomes.
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http://dx.doi.org/10.1002/onco.13687DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100562PMC
May 2021

From Polyclonal Sera to Recombinant Antibodies: A Review of Immunological Detection of Gluten in Foodstuff.

Foods 2020 Dec 30;10(1). Epub 2020 Dec 30.

Departamento de Nutrición y Ciencia de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain.

Gluten is the ethanol-soluble protein fraction of cereal endosperms like wheat, rye, and barley. It is widely used in the food industry because of the physical-chemical properties it gives to dough. Nevertheless, there are some gluten-related diseases that are presenting increasing prevalences, e.g., celiac disease, for which a strict gluten-free diet is the best treatment. Due to this situation, gluten labeling legislation has been developed in several countries around the world. This article reviews the gluten immune detection systems that have been applied to comply with such regulations. These systems have followed the development of antibody biotechnology, which comprise three major methodologies: polyclonal antibodies, monoclonal antibodies (mAbs) derived from hybridoma cells (some examples are 401.21, R5, G12, and α-20 antibodies), and the most recent methodology of recombinant antibodies. Initially, the main objective was the consecution of new high-affinity antibodies, resulting in low detection and quantification limits that are mainly achieved with the R5 mAb (the gold standard for gluten detection). Increasing knowledge about the causes of gluten-related diseases has increased the complexity of research in this field, with current efforts not only focusing on the development of more specific and sensitive systems for gluten but also the detection of protein motifs related to pathogenicity. New tools based on recombinant antibodies will provide adequate safety and traceability methodologies to meet the increasing market demand for gluten-free products.
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http://dx.doi.org/10.3390/foods10010066DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7824297PMC
December 2020

Production of a Recombinant Single-Domain Antibody for Gluten Detection in Foods Using the Expression System.

Foods 2020 Dec 10;9(12). Epub 2020 Dec 10.

Departamento de Nutrición y Ciencia de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain.

The detection of gluten in foodstuffs has become a growing concern in food allergen management as a result of the high ratio of population sensitive to the main gluten-containing cereals. In this study, a promising single-domain antibody previously isolated by phage display (dAb8E) was produced in resulting in high levels of the antibody fragment expression (330 mg/L). The purified dAb8E was proved to specifically bind to gluten proteins from wheat, barley and rye, exhibiting no cross reaction to other heterologous species. The dynamic range of the sandwich enzyme-linked immunosorbent assay (ELISA) covered 0.1 to 10 µg/mL of gliadin, reaching a limit of detection of 0.12 µg/mL. When experimental binary mixtures of the target cereals were analyzed, the limit of detection was 0.13 mg/g, which would theoretically correspond to gluten concentrations of approximately 13 mg/kg. Finally, thirty commercially available food products were analyzed by means of the developed assay to further confirm the applicability of the dAb8E for gluten determination. The proposed methodology enabled the generation of a new gluten-specific nanobody which could be used to guarantee the appropriate labelling of gluten-free foods.
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http://dx.doi.org/10.3390/foods9121838DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7764234PMC
December 2020

A Variant in a Litter of Neonatal Basset Hounds with Dystrophic Epidermolysis Bullosa.

Genes (Basel) 2020 12 4;11(12). Epub 2020 Dec 4.

Institute of Genetics, Vetsuisse Faculty, University of Bern, 3001 Bern, Switzerland.

We investigated three neonatal Basset Hound littermates with lesions consistent with epidermolysis bullosa (EB), a group of genetic blistering diseases. A clinically normal bitch was bred to her grandfather by artificial insemination. Out of a litter of seven puppies, two affected puppies died and one was euthanized, with these puppies being submitted for diagnostic necropsy. All had multiple bullae and ulcers involving the nasal planum and paw pads, as well as sloughing claws; one puppy also had oral and esophageal ulcers. The complete genome of one affected puppy was sequenced, and 37 known EB candidate genes were assessed. We found a candidate causative variant in , which encodes the collagen VII alpha 1 chain. The variant is a complex rearrangement involving duplication of a 107 bp region harboring a frameshift deletion of 7 bp. The variant is predicted to truncate more than 75% of the open reading frame, p.(Val677Serfs*11). Targeted genotyping of this duplication confirmed that all three affected puppies were homozygous for the duplication, whereas 12 unaffected Basset Hounds did not carry the duplication. This variant was also not seen in the genomes of more than 600 dogs of other breeds. variants have been identified in humans and dogs with dystrophic epidermolysis bullosa (DEB). The identified variant therefore most likely represents the causative variant and allows the refinement of the preliminary EB diagnosis to DEB.
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http://dx.doi.org/10.3390/genes11121458DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7762066PMC
December 2020

Study protocol of a pilot study evaluating feasibility and acceptability of a psychosexual intervention for couples postallogeneic haematopoietic stem cell transplantation.

BMJ Open 2020 10 31;10(10):e039300. Epub 2020 Oct 31.

Psychosocial Oncology Program, Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia.

Introduction: Sexual dysfunction is one of the most common side effects of allogeneic haematopoietic stem cell transplantation (HSCT) for haematological cancers. Problems can persist between 5 and 10 years post-transplant and impact mood, couple intimacy and relationship satisfaction. Few intervention studies, however, target sexual dysfunction in patients post-HSCT. This pilot study aims to examine the feasibility and acceptability of implementing a psychosexual intervention for HSCT survivors and their partners post-transplantation.

Methods And Analysis: Fifteen allogeneic HSCT survivors and their partners will be recruited. Patients who are more than 3 months post-transplantation will be sent invitation letters describing the couples' psychosexual intervention that will be offered through this study. The intervention will comprise two components: (1) psychosexual education about medical and behavioural treatment options for sexual dysfunction delivered by a haematology nurse consultant; (2) emotionally focused therapy-based relationship education programme for couples delivered by a clinical psychologist (four sessions of 1.5 hours each). Couples who consent to participate will be administered a series of measures assessing mood, relationship satisfaction and sexual dysfunction preintervention and post-intervention, as well as satisfaction with the intervention postintervention. Feasibility of the intervention will be examined via recording enrolment rate, adherence, compliance with completing outcome measures and fidelity of intervention delivery.

Ethics And Dissemination: Ethics approval has been obtained at the Peter MacCallum Cancer Centre in Melbourne, Australia. Results will be presented at national and international conferences and published in a peer-reviewed journal so that in can be accessed by clinicians involved in the care of allogeneic HSCT patients. If this intervention is found to be feasible and acceptable, its impact will be examined in a future randomised controlled trial and subsequently implemented as part of routine care in the allogeneic HSCT population.
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http://dx.doi.org/10.1136/bmjopen-2020-039300DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7783613PMC
October 2020

Phage Displayed Domain Antibodies (dAb) for Detection of Allergenic Pistachio Proteins in Foods.

Foods 2020 Sep 3;9(9). Epub 2020 Sep 3.

Departamento de Nutrición y Ciencia de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain.

Pistachio nuts () have been consumed by past and present-day civilizations because of their organoleptic characteristics and potential health benefits. However, they can also produce moderate to severe IgE-mediated reactions in allergic individuals. In this work, we report the isolation of the first recombinant antibodies against pistachio nut, produced without animal immunization, to be used in immunoassays for detection of allergenic pistachio in food products. Several phage display biopanning strategies were evaluated to screen the human-based domain antibody library (dAb) in search for pistachio-specific probes. The clone producing the PVF4 phage-dAb was finally selected, and it does not cross-react with cashew despite the phylogenetic proximity with pistachio. Western blot and matrix-assisted laser desorption/ionization tandem mass spectrometry (MALDI-TOF/TOF) analysis demonstrated that this clone recognised a unique band of ∼22 kDa related to the basic subunit of pistachio 11S globulin (allergen Pis v 2). The PVF4 phage-dAb allowed detection of pistachio in a food matrix with a limit of detection (LOD) of 3983 mg kg-1 in an indirect phage-enzyme-linked immunosorbent assay (ELISA). The ELISA method developed was used to assess applicability of the PVF4 phage-dAb for analysis of 77 commercial food products.
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http://dx.doi.org/10.3390/foods9091230DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7554873PMC
September 2020

Undifferentiated carcinoma with osteoclast-like giant cells of the pancreas diagnosed by endoscopic ultrasound guided biopsy.

Ecancermedicalscience 2020 17;14:1072. Epub 2020 Jul 17.

Department of Histopathology, Hospital Británico, Buenos Aires C1280AEB, Argentina.

Undifferentiated pancreatic carcinoma with osteoclast-like giant cells is a rare tumour that has been published under a wide variety of names, including pleomorphic carcinoma, giant cell carcinoma, sarcomatoid carcinoma and carcinosarcoma, among others. For these reasons and its low frequency, the reports of these tumours are scarce and frequently lead to confusion with other entities which present with giant cells. We present the case of a patient with obstructive jaundice and a mixed cystic and solid pancreatic mass, accompanied by multiple hepatic lesions. The histological study of the material obtained by endoscopic ultrasound guided biopsy demonstrated a proliferation of atypical epithelioid cells, accompanied by a spindle cell component with marked pleomorphism and numerous osteoclast-like giant cells. The epithelioid component showed positive immunostaining with cytokeratin cocktail and cytokeratin 7. The spindle cell component showed coexpression of cytokeratins and vimentin. The osteoclast-like giant cells were positive for CD68. Protein p53 was overexpressed in both epithelial and spindle cell neoplastic components, and was negative in the giant cells. These findings permitted the diagnosis of undifferentiated carcinoma of the pancreas with osteoclast-like giant cells. This case outlines the effectiveness of endoscopic ultrasound-guided biopsy and the importance of morphological and immunohistochemical examination in the diagnosis of different types of pancreatic tumours, especially when they are in advanced stages and are not suitable for surgical treatment.
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http://dx.doi.org/10.3332/ecancer.2020.1072DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7434513PMC
July 2020

Vapor-Phase Photocatalytic Overall Water Splitting Using Hybrid Methylammonium Copper and Lead Perovskites.

Nanomaterials (Basel) 2020 May 18;10(5). Epub 2020 May 18.

Instituto Universitario de Tecnología Química CSIC-UPV, Universidad Politecnica de Valencia, Av. de los Naranjos s/n, 46022 Valencia, Spain.

Films or powders of hybrid methylammonium copper halide perovskite exhibit photocatalytic activity for overall water splitting in the vapor phase in the absence of any sacrificial agent, resulting in the generation of H and O, reaching a maximum production rate of 6 μmol H × g cath efficiency. The photocatalytic activity depends on the composition, degreasing all inorganic CsCuClBr perovskite and other Cl/Br proportions in the methylammonium hybrids. XRD indicates that MACuClBr is stable under irradiation conditions in agreement with the linear H production with the irradiation time. Similar to copper analogue, hybrid methylammonium lead halide perovskites also promote the overall photocatalytic water splitting, but with four times less efficiency than the Cu analogues. The present results show that, although moisture is strongly detrimental to the photovoltaic applications of hybrid perovskites, it is still possible to use these materials as photocatalysts for processes requiring moisture due to the lack of relevance in the photocatalytic processes of interparticle charge migration.
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http://dx.doi.org/10.3390/nano10050960DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7279556PMC
May 2020

Impact of physical exercise in patients with chronic kidney disease: Sistematic review and meta-analysis.

Nefrologia (Engl Ed) 2020 May - Jun;40(3):237-252. Epub 2020 Apr 15.

Servicio de Nefrología, Hospital Universitario Puerta del Mar, Cádiz, España.

Physical exercise may offer multiple benefits to patients with chronic kidney disease (CKD). However, it was not traditionally recommended because of the possibility of impairing renal function and increasing proteinuria. The objective of this study is to review the clinical trials on physical exercise in patients with CKD and describe its effect on the progression of kidney disease and other factors associated. Randomized clinical trials (RCT) comparing an intervention that included an exercise component with a control group without physical exercise in non-dialysis patients with CKD from 2007 to 2018 in English and Spanish were included. PubMed, Scopus, Embase, Ovid (Medline) and PEDro databases were used for the search. Effects of physical exercise were summarized by the standardized mean difference (SMD). No differences were found in glomerular filtration rate or proteinuria between the intervention group and the control group: SMD -0.3 (P=.81); SMD 26.6 (P=.82). Positive effects were obtained on peak oxygen consumption: SMD 2.5 (P<.001), functional capacity: SMD 56.6 (P<.001), upper limb strength: SMD 6.8 (P<.001) and hemoglobin: SMD 0.3 (P=.003). An improvement on the quality of life was also evident using the KDQOL-36 survey: SMD 3.56 (P=.02) and the SF-36 survey: SMD 6.66 (P=.02). In conclusion, the practice of low-intensity physical exercise routinely has no negative impact on renal function. On the contrary, it improves aerobic and functional capacity, impacting positively on the quality of life.
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http://dx.doi.org/10.1016/j.nefro.2020.01.002DOI Listing
June 2021

Thermodynamic and Evolutionary Coupling between the Native and Amyloid State of Globular Proteins.

Cell Rep 2020 04;31(2):107512

Switch Laboratory, VIB Center for Brain and Disease Research, Herestraat 49, 3000 Leuven, Belgium; Switch Laboratory, Department of Cellular and Molecular Medicine, KU Leuven, Herestraat 49, 3000 Leuven, Belgium. Electronic address:

The amyloid-like aggregation propensity present in most globular proteins is generally considered to be a secondary side effect resulting from the requirements of protein stability. Here, we demonstrate, however, that mutations in the globular and amyloid state are thermodynamically correlated rather than simply associated. In addition, we show that the standard genetic code couples this structural correlation into a tight evolutionary relationship. We illustrate the extent of this evolutionary entanglement of amyloid propensity and globular protein stability. Suppressing a 600-Ma-conserved amyloidogenic segment in the p53 core domain fold is structurally feasible but requires 7-bp substitutions to concomitantly introduce two aggregation-suppressing and three stabilizing amino acid mutations. We speculate that, rather than being a corollary of protein evolution, it is equally plausible that positive selection for amyloid structure could have been a driver for the emergence of globular protein structure.
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http://dx.doi.org/10.1016/j.celrep.2020.03.076DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7175379PMC
April 2020

A novel approach to produce phage single domain antibody fragments for the detection of gluten in foods.

Food Chem 2020 Aug 23;321:126685. Epub 2020 Mar 23.

Departamento de Nutrición y Ciencia de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain.

In this study, we demonstrated the feasibility of isolating recombinant phage-antibodies against gluten from a non-immunized library of human single-domain antibodies (dAbs). Phage display technology enabled the selection of affinity probes by successive rounds of biopanning against a biotinylated synthetic peptide comprising repetitive immunogenic gluten motifs. The analysis of a wide representation of heterologous plant species corroborated that two of the isolated clones were specific to wheat, barley and rye proteins. The phage antibody selected as the most appropriate clone for the detection of gluten in foods (dAb8E-phage) was further applied in an indirect ELISA to the analysis of 50 commercial food samples. Although the limit of detection achieved did not improve those of current immunoassays, the proposed methodology could provide promising new pathways for the generation of recombinant antibodies that allow a comprehensive determination of gluten in foods, whilst replacing the need for animal immunization.
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http://dx.doi.org/10.1016/j.foodchem.2020.126685DOI Listing
August 2020

Direct-Acting Antiretroviral Therapy in Renal Transplant Recipients With Human Immunodeficiency Virus-Hepatitis C Virus Coinfection: Report of Our Experience and Literature Review.

Transplant Proc 2020 Mar 6;52(2):523-526. Epub 2020 Feb 6.

Department of Nephrology, Hospital Puerta del Mar, Cadiz, Spain.

A minor graft and patient survival are described in renal transplant recipients with hepatitis C virus (HCV) and human immunodeficiency virus (HIV) coinfection than in recipients infected with only HIV. The high efficacy of direct-acting antivirals could improve the results. The experience reported in renal transplant recipients with coinfection is very limited.

Material And Methods: We analyzed the evolution of renal recipients with HIV-HCV coinfection treated with direct-acting antivirals in our center. Clinical, analytical, and microbiological variables were collected before and after treatment.

Results: From 2001 to 2018 we performed 11 renal transplants in patients with HIV infection, and 6 (54.5%) had HIV-HCV coinfection. One patient lost the graft before the development of direct-acting antivirals. Another patient with functioning graft has refused to receive any treatment. Four patients have been treated with direct-acting antivirals. One was treated 18 months before the transplant; 3 received treatment after transplant. All received sofosbuvir-based therapies. All had a sustained virologic response after 12 weeks and an improvement of liver function. In the patients treated after renal transplant, time post transplant at the beginning of treatment was 99.6 (SD, 22.8) months, and follow-up after treatment in all patients was 40.2 (SD, 8.16) months. To modify immunosuppressive regimen was not necessary, although 2 patients required an increase of tacrolimus doses. We do not observe deterioration of renal function. All have maintained a good immunologic and microbiological control without requiring changes in antiretrovirals. We do not observe complications associated with treatment.

Conclusions: Direct-acting antivirals therapy is safe and effective and may offer new possibilities to patients with HIV-HCV coinfection.
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http://dx.doi.org/10.1016/j.transproceed.2019.12.009DOI Listing
March 2020

Acute Renal Failure in Kidney Transplantation Due to Subcapsular Hematoma After a Renal Allograft Biopsy: Report of Two Cases and Literature Review.

Transplant Proc 2020 Mar 5;52(2):530-533. Epub 2020 Feb 5.

Department of Nephrology, Hospital Universitario Puerta del Mar, Cádiz, Spain.

The compression of the renal parenchyma due to a subcapsular hematoma, also known as the "Page kidney," is a potentially serious but treatable complication of renal biopsy. Hypertension is very common and, in some cases, renal failure may be present. In kidney transplantation, it is a not well-described entity. Rapid intervention is essential to avoid irreversible damage of the graft and preserve its function. We report 2 cases of acute renal failure due to Page kidney in patients with renal transplant after a percutaneous biopsy with successful recovery after surgical treatment. In addition, we conducted a literature review in order to describe the clinical characteristics of this infrequent complication in patients with a history of renal transplant.
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http://dx.doi.org/10.1016/j.transproceed.2019.09.018DOI Listing
March 2020

Characteristics and Evolution of Renal Transplant Recipients Infected by Carbapenemase-Producing Klebsiella pneumoniae.

Transplant Proc 2020 Mar 3;52(2):519-522. Epub 2020 Feb 3.

Department of Nephrology, Hospital Puerta del Mar, Cadiz, Spain.

Background: Renal transplant (RT) recipients are especially susceptible to carbapenem-resistant Klebsiella pneumoniae carbapenemase (KPC) infections. However, published experience is limited.

Objective: To analyze the characteristics and evolution of RT recipients with KPC infection in our hospital.

Methods: We performed a retrospective cohort study of all RT recipients with KPC infection in our hospital from December 1, 2017 (first case), to July 31, 2019. For each RT recipient infected with KPC, 3 controls were selected.

Results: During the study period, 8 RT recipients presented KPC infection. Seven were detected in the first year post-RT. The most common site of infection was urine. In 2 cases the germ was isolated in blood. The number of patients with diabetes was significantly higher in the group with KPC infection (P = .023), and urologic interventions were more frequent in those patients (P = .039). No differences were found in the immunosuppressive treatment. A total of 62.5 % of patients required readmission after the KPC infection. One patient died of septicemia by KPC. In all these cases, the clone of KPC isolated was KPC ST512.

Conclusion: KPC infection is more frequent in the first months after the RT and causes an important number of hospital admissions. It can be cause of death in RT recipients, especially in those with isolation of the germ in blood. Diabetes and urologic interventions were more frequent in this population. The analysis by molecular typing suggests exposure to a common source, highlighting the importance of preventive isolation measures and surveillance for limiting the transmission of this bacteria.
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http://dx.doi.org/10.1016/j.transproceed.2019.09.017DOI Listing
March 2020

Young Children Are Wishful Thinkers: The Development of Wishful Thinking in 3- to 10-Year-Old Children.

Child Dev 2020 07 9;91(4):1166-1182. Epub 2019 Aug 9.

University of California, Berkeley.

Previously, research on wishful thinking has found that desires bias older children's and adults' predictions during probabilistic reasoning tasks. In this article, we explore wishful thinking in children aged 3- to 10-years-old. Do young children learn to be wishful thinkers? Or do they begin with a wishful thinking bias that is gradually overturned during development? Across five experiments, we compare low- and middle-income United States and Peruvian 3- to 10-year-old children (N = 682). Children were asked to make predictions during games of chance. Across experiments, preschool-aged children from all backgrounds consistently displayed a strong wishful thinking bias. However, the bias declined with age.
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http://dx.doi.org/10.1111/cdev.13299DOI Listing
July 2020

Rhamnolipids functionalized with basic amino acids: Synthesis, aggregation behavior, antibacterial activity and biodegradation studies.

Colloids Surf B Biointerfaces 2019 Sep 18;181:234-243. Epub 2019 May 18.

Department of Surfactant and Nanobiotechnology, IQAC-CSIC, c/ Jordi Girona, 18-26, 08034 Barcelona, Spain. Electronic address:

Rhamnolipids have been intensively studied due to their remarkable properties; however, the biosynthesis of RLs cannot compete commercially with the production of synthetic surfactants. Here, novel cationic rhamnolipids (RLs) derivatives containing arginine and lysine were prepared for the first time using a straightforward synthetic procedure. The RLs used to prepare these new cationic derivatives were produced by Pseudomonas aeruginosa using waste frying oil as carbon source. It was found that the amino acid-based RLs form aggregates at very low concentrations, even below the CMC. Biodegradation studies indicate that these cationic RLs can be classified as readily biodegradable. Interestingly, the RL arginine conjugates exhibited notable DNA binding affinity and good antimicrobial activity against Gram-positive bacteria, including methicillin-resistant Staphylococcus aureus, which increases the potential applications of these compounds. Consequently, the use of low-cost substrates and the added value of the final product constitute a more cost-effective rhamnolipid production.
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http://dx.doi.org/10.1016/j.colsurfb.2019.05.037DOI Listing
September 2019

Biodegradability and aquatic toxicity of new cleavable betainate cationic oligomeric surfactants.

J Hazard Mater 2019 06 1;371:108-114. Epub 2019 Mar 1.

Laboratory of Microbiocides Chemistry, Faculty of Chemistry, Adam Mickiewicz University, Umultowska 89b, 61-614, Poznań, Poland. Electronic address:

New cleavable oligomeric cationic surfactants containing ester groups susceptible to hydrolysis between the hydrocarbon tails and the hydrophilic moiety have been synthesized and their biodegradability and aquatic toxicity examined. Aerobic biodegradability was evaluated by applying a standard method for ready biodegradability, the CO Headspace test. Aquatic toxicity was assessed by means of the acute toxicity test with Daphnia. Cleavable oligomeric cationic surfactants undergo a significant biodegradation extent (31-52%) as compared to dimeric surfactants without ester groups that showed null degradation in previous works. However, they do not attain the threshold of ultimate degradation required (60%) to be classed as easily biodegradable chemicals. On the other hand, the introduction of cleavable groups in the surfactant hydrophobic chains reduces the toxic effects on the microorganisms responsible for degradation observed for conventional alkyl ammonium dimeric surfactants. Acute toxicity values of betainate cationic oligomeric surfactants to Daphnia magna, IC-48 h, varies from 1.5 to 50 mg/L. Aquatic toxicity of oligomeric cationic surfactants depends on their hydrophobicity and increases regularly with the alkyl chain length. However, whether the surfactant is a dimeric or a trimeric betaine ester does not affect their acute toxicity to crustacean.
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http://dx.doi.org/10.1016/j.jhazmat.2019.03.005DOI Listing
June 2019

Chiral hybrid materials based on pyrrolidine building units to perform asymmetric Michael additions with high stereocontrol.

Catal Sci Technol 2018 Nov 23;8(22):5835-5847. Epub 2018 Oct 23.

Instituto de Tecnología Química , Universitat Politècnica de València-Consejo Superior de Investigaciones Científicas , Avenida de los Naranjos s/n , E-46022 Valencia , Spain . Email: ; Email: ; ; Tel: +34963877800.

A new chiral mesoporous hybrid material was synthesized based on pyrrolidine units included in a siliceous framework, HybPyr, and integrated into the organic-inorganic structure, from a specific bis-silylated precursor. A fluoride sol-gel methodology under soft synthesis conditions and in the absence of sophisticated structural directing agents allowed the generation of a mesoporous architecture with a homogeneous distribution of active chiral moieties along the network. The hybrid material was studied by means of different characterization techniques (TGA, NMR and IR spectroscopy, chemical and elemental analyses, TEM, and textural measurements), verifying the stability and integrity of the asymmetric active sites in the solid. The hybrid material, HybPyr, is an excellent asymmetric heterogeneous and recyclable catalyst for enantioselective Michael addition of linear aldehydes to β-nitrostyrene derivatives with high stereocontrol of the reaction products.
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http://dx.doi.org/10.1039/c8cy01650jDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6333262PMC
November 2018

Effect of a Lifestyle Intervention Program With Energy-Restricted Mediterranean Diet and Exercise on Weight Loss and Cardiovascular Risk Factors: One-Year Results of the PREDIMED-Plus Trial.

Diabetes Care 2019 05 2;42(5):777-788. Epub 2018 Nov 2.

Centro de Investigación Biomédica en Red de Fisiopatología de la Obesidad y la Nutrición (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain.

Objective: The long-term impact of intentional weight loss on cardiovascular events remains unknown. We describe 12-month changes in body weight and cardiovascular risk factors in PREvención con DIeta MEDiterránea (PREDIMED)-Plus, a trial designed to evaluate the long-term effectiveness of an intensive weight loss lifestyle intervention on primary cardiovascular prevention.

Research Design And Methods: Overweight/obese adults with metabolic syndrome aged 55-75 years ( = 626) were randomized to an intensive weight loss lifestyle intervention based on an energy-restricted Mediterranean diet, physical activity promotion, and behavioral support (IG) or a control group (CG). The primary and secondary outcomes were changes in weight and cardiovascular risk markers, respectively.

Results: Diet and physical activity changes were in the expected direction, with significant improvements in IG versus CG. After 12 months, IG participants lost an average of 3.2 kg vs. 0.7 kg in the CG ( < 0.001), a mean difference of -2.5 kg (95% CI -3.1 to -1.9). Weight loss ≥5% occurred in 33.7% of IG participants compared with 11.9% in the CG ( < 0.001). Compared with the CG, cardiovascular risk factors, including waist circumference, fasting glucose, triglycerides, and HDL cholesterol, significantly improved in IG participants ( < 0.002). Reductions in insulin resistance, HbA, and circulating levels of leptin, interleukin-18, and MCP-1 were greater in IG than CG participants ( < 0.05). IG participants with prediabetes/diabetes significantly improved glycemic control and insulin sensitivity, along with triglycerides and HDL cholesterol levels compared with their CG counterparts.

Conclusions: PREDIMED-Plus intensive lifestyle intervention for 12 months was effective in decreasing adiposity and improving cardiovascular risk factors in overweight/obese older adults with metabolic syndrome, as well as in individuals with or at risk for diabetes.
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http://dx.doi.org/10.2337/dc18-0836DOI Listing
May 2019

Neutralization of IL-17C Reduces Skin Inflammation in Mouse Models of Psoriasis and Atopic Dermatitis.

J Invest Dermatol 2018 07 21;138(7):1555-1563. Epub 2018 Feb 21.

MorphoSys AG, Planegg, Germany.

IL-17C is a functionally distinct member of the IL-17 family that was believed to play a role in the pathogenesis of psoriasis. Here we confirmed that IL-17C is involved in psoriasis and explored potential roles for IL-17C in atopic dermatitis (AD). An anti-IL-17C antibody, MOR106, was generated that potently and selectively binds to human and mouse IL-17C, thereby inhibiting the binding of IL-17C to its IL-17RE receptor. The antibody inhibited cutaneous inflammation in an IL-23-induced psoriatic-like skin inflammation model. In lesional skin of patients with AD, IL-17C expression levels were increased and localized to keratinocytes and infiltrating immune cells. To determine the contribution of IL-17C to AD pathogenesis, MOR106 was tested in two distinct in vivo models. In the calcipotriol-induced AD model, ear skin inflammation, TSLP, and IL-33 protein production in ears was suppressed by MOR106. Consistently, in the flaky tail strain mouse model, spontaneous development of AD-like skin inflammation was reduced by MOR106. Moreover, serum IgE levels, number of mast cells in skin and T helper type 2-related cytokines IL-4 and CCL17 in serum were all reduced. Overall, our results indicate that IL-17C is a central mediator of skin inflammation beyond psoriasis and is relevant in particular in AD.
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http://dx.doi.org/10.1016/j.jid.2018.01.036DOI Listing
July 2018

Efficacy and safety of chemotherapy in older versus non-older patients with advanced gastric cancer: A real-world data, non-inferiority analysis.

J Geriatr Oncol 2018 05 11;9(3):254-264. Epub 2017 Dec 11.

Hematology and Medical Oncology Department, Hospital Universitario Morales Meseguer, Murcia, Spain.

Objective: Advanced gastric cancer (AGC) is a common neoplasm in older adults. Nevertheless, there are few specific management data in the literature. The aim of this study was to assess non-inferiority of survival and efficacy-related outcomes of chemotherapy used in older vs non-older patients with AGC.

Materials And Methods: We recruited 1485 patients from the AGAMENON registry of AGC treated with polychemotherapy between 2008-2017. A statistical analysis was conducted to prove non-inferiority for overall survival (OS) associated with the use of chemotherapy schedules in individuals ≥70 vs.<70years. The fixed-margin method was used (hazard ratio [HR]<1.176) that corresponds to conserving at least 85% efficacy.

Results: 33% (n=489) of the cases analyzed were ≥70 years. Two-agent chemotherapies and combinations with oxaliplatin (48% vs. 29%) were used more often in the older patients, as were modified schedules and/or lower doses. Toxicity grade 3-4 was comparable in both groups, although when looking at any grade, there were more episodes of enteritis, renal toxicity, and fatigue in older patients. In addition, toxicity was a frequent cause for discontinuing treatment in older patients. The response rate was similar in both groups. After adjusting for confounding factors, the non-inferiority of OS associated with schedules administered to the older vs. younger subjects was confirmed: HR 1.02 (90% CI, 0.91-1.14), P (non inferiority)=0.018, as well as progression-free survival: HR 0.97 (90% CI, 0.87-1.08), P(non-inferiority)=0.001.

Conclusion: In this AGC registry, the use of chemotherapy with schedules adapted to patients ≥70 years provided efficacy that was not inferior to that seen in younger cases, with comparable adverse effects.
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http://dx.doi.org/10.1016/j.jgo.2017.11.008DOI Listing
May 2018

Long-Term Underwater Hydrophobicity: Exploring Topographic and Chemical Requirements.

ACS Omega 2017 Dec 13;2(12):8928-8939. Epub 2017 Dec 13.

Instituto de Ciencia y Tecnología de Polímeros, ICTP-CSIC, Juan de la Cierva 3, 28006 Madrid, Spain.

A family of hybrid organoinorganic silica-based particles with varied chemical natures and morphologies has been synthesized to test their ability to develop coatings with underwater hydrophobicity. The particles were characterized by elemental microanalysis, scanning electron microscopy, and dynamic light scattering to evaluate the organic content, observe the morphology, and estimate the aggregate size, respectively. These morphologies were transferred into surface topographies by spraycoating dispersions made from the particles onto glass supports, resulting in coatings with an ample range of profiles and roughness but all of them being superhydrophobic. Atomic force microscopy and optical profilometry were used to map the coating surfaces and analyze the topography. Then, underwater hydrophobicity endurance was tested by immersion under a 2 cm 20 °C water column perpendicular to circular glass supports coated with the particles. The so-called mirror effect derived from the occurrence of the primary plastron (continuous air layer occluded between the surface and the water) was observed on the surface of all of the coatings tested. Apart from the dependency of plastrons on the water temperature and substrate shape, the plastron quality and lifetime is notably different depending on the particle morphology and thus on the coating topography. These experiments have demonstrated that the most persistent mirror effects, and therefore underwater superhydrophobicity, were produced on coatings that exhibited the smoothest topographies at the micrometric scale. In addition, these particle-only coatings can be made mechanically stable and robust by blending with a polymer matrix.
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http://dx.doi.org/10.1021/acsomega.7b01717DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6645558PMC
December 2017
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