Publications by authors named "Tatiana Orlova"

11 Publications

  • Page 1 of 1

Aquatic toxicity of particulate matter emitted by five electroplating processes in two marine microalgae species.

Toxicol Rep 2021 16;8:880-887. Epub 2021 Apr 16.

N.I. Vavilov All-Russian Institute of Plant Genetic Resources, Saint-Petersburg, 190000, Russia.

Electroplating is a widely used group of industrial processes that make a metal coating on a solid substrate. Our previous research studied the concentrations, characteristics, and chemical composition of nano- and microparticles emitted during different electroplating processes. The objective of this study was to evaluate the environmental toxicity of particulate matter obtained from five different electrochemical processes. We collected airborne particle samples formed during aluminum cleaning, aluminum etching, chemical degreasing, nonferrous metals etching, and nickel plating. The toxicity of the particles was evaluated by the standard microalgae growth rate inhibition test. Additionally, we evaluated membrane potential and cell size changes in the microalgae and exposed to the obtained suspensions of electroplating particles. The findings of this research demonstrate that the aquatic toxicity of electroplating emissions significantly varies between different industrial processes and mostly depends on particle chemical composition and solubility rather than the number of insoluble particles. The sample from an aluminum cleaning workshop was significantly more toxic for both microalgae species compared to the other samples and demonstrated dose and time-dependent toxicity. The samples obtained during chemical degreasing and nonferrous metals etching processes induced depolarization of microalgal cell membranes, demonstrated the potential of chronic toxicity, and stimulated the growth rate of microalgae after 72 h of exposure. Moreover, the sample from a nonferrous metals etching workshop revealed hormetic dose-response toxicity in , which can lead to harmful algal blooms in the environment.
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http://dx.doi.org/10.1016/j.toxrep.2021.04.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085665PMC
April 2021

Harmful algal blooms and associated fisheries damage in East Asia: Current status and trends in China, Japan, Korea and Russia.

Harmful Algae 2021 Feb 23;102:101787. Epub 2020 Mar 23.

Asian Natural Environmental Science Center, The University of Tokyo, Tokyo 113-8657, Japan. Electronic address:

Occurrences of harmful algal blooms (HABs) and associated fisheries damage have been continuously monitored since the 1970s along the coasts of East Asia. Fisheries damage comprises mass mortalities of fish and shellfish mainly by harmful dinoflagellates and raphidophytes (e.g., Chattonella antiqua/marina, Cochlodinium polykrikoides and Karenia mikimotoi), and contamination of algal toxins in shellfish in particular Diarrhetic Shellfish Toxins by Dinophysis spp. and Paralytic Shellfish Toxins by Alexandrium spp. Shellfish mass mortalities due to Heterocapsa circularisquama in Hong Kong and western Japan, and fish kills by Karlodinium digitatum are unique incidents for this region, whereas C. antiqua/marina, C. polykrikoides and K. mikimotoi are common also in other regions. Time series data showed that the highest bloom numbers were recorded in 1980 (Japan), in 1998 (Korea) and in 2003 (China), followed by decreasing trends in these countries. These data suggest a shift in microalgal species composition, from dominance by diatoms to dinoflagellates after 1980s in Korea, and from diatoms to small haptophytes and cyanobacteria after 2013 in eastern Russia. HAB species composition and the changes were compared among countries, for better understanding on current status and trend of HAB species in East Asia.
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http://dx.doi.org/10.1016/j.hal.2020.101787DOI Listing
February 2021

Single crystals of ferroelectric lithium niobate-tantalate LiNbTaO solid solutions for high-temperature sensor and actuator applications.

Acta Crystallogr B Struct Sci Cryst Eng Mater 2020 Dec 16;76(Pt 6):1071-1076. Epub 2020 Nov 16.

Universität Potsdam, Institut für Physik, Karl Liebknecht Str. 24-25, Potsdam, 14471, Germany.

Ferroelectric LiNbTaO solid solutions with various Nb/Ta ratio were grown from the melt by the Czochralski method. The exact composition of the grown crystals was determined by inductively coupled plasma atomic mass spectrometry. The dependence of the crystal composition on the composition of the initial melt was obtained and explained by a wide separation between the phase boundaries of the liquid and solid phases on the LiNbO-LiTaO phase diagram. Using high-resolution X-ray diffraction, the parameters a and c of a crystal unit cell were determined (LiNbTaO: a = 5.1574 Å and c = 13.8498 Å). Further, the Curie temperature T of the crystals was measured using the differential scanning calorimetry technique. T was found to depend on the composition of the crystals that allowed conditions for the monodomainization of the grown crystals to be defined (LiNbTaO: T = 1102°C; LiNbTaO: T = 794°C). Finally, the velocity of surface acoustic waves was determined by scanning electron microscopy and X-ray diffraction techniques (YZ-cut of a LiNbTaO crystal: V = 3440 m s).
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http://dx.doi.org/10.1107/S2052520620014390DOI Listing
December 2020

Morphological and phylogenetic data do not support the split of Alexandrium into four genera.

Harmful Algae 2020 09 18;98:101902. Epub 2020 Sep 18.

MARBEC, Université de Montpellier, CNRS, Ifremer, IRD, Montpellier, France.

A recently published study analyzed the phylogenetic relationship between the genera Centrodinium and Alexandrium, confirming an earlier publication showing the genus Alexandrium as paraphyletic. This most recent manuscript retained the genus Alexandrium, introduced a new genus Episemicolon, resurrected two genera, Gessnerium and Protogonyaulax, and stated that: "The polyphyly [sic] of Alexandrium is solved with the split into four genera". However, these reintroduced taxa were not based on monophyletic groups. Therefore this work, if accepted, would result in replacing a single paraphyletic taxon with several non-monophyletic ones. The morphological data presented for genus characterization also do not convincingly support taxa delimitations. The combination of weak molecular phylogenetics and the lack of diagnostic traits (i.e., autapomorphies) render the applicability of the concept of limited use. The proposal to split the genus Alexandrium on the basis of our current knowledge is rejected herein. The aim here is not to present an alternative analysis and revision, but to maintain Alexandrium. A better constructed and more phylogenetically accurate revision can and should wait until more complete evidence becomes available and there is a strong reason to revise the genus Alexandrium. The reasons are explained in detail by a review of the available molecular and morphological data for species of the genera Alexandrium and Centrodinium. In addition, cyst morphology and chemotaxonomy are discussed, and the need for integrative taxonomy is highlighted.
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http://dx.doi.org/10.1016/j.hal.2020.101902DOI Listing
September 2020

Development of a Process for Color Improvement of Low-Grade Dark Maple Syrup by Adsorption on Activated Carbon.

ACS Omega 2020 Aug 12;5(33):21084-21093. Epub 2020 Aug 12.

Department of Soil Sciences and Agro-Food Engineering, Université Laval, Quebec, Quebec G1V 0A6, Canada.

Low-grade dark maple syrup was successfully discolored on activated carbon. Several experimental parameters were tested, namely, the mixing time (20, 40, and 60 min), concentration of the activated carbon (0.1, 0.3, and 0.5 g/100 mL), type of activated carbon (I, II, and III), activated carbon particle size (25, 50, and 75 μm), stirring speed (200, 400, and 600 rpm), and temperature (40, 60, and 80 °C). The obtained results showed that the discoloration is optimal by applying the following parameters: a mixing time of 40 min with a type III activated carbon at a concentration of 0.3 g/100 mL. These parameters yielded a light transmittance at 560 nm of 83.70 ± 0.21%, which ranks the syrup in the extra clear class according to the Canadian classification. The results showed that among the tested carbons, the adsorption on the type III carbon followed the Langmuir, Freundlich, and Langmuir-Freundlich adsorption isotherms. Regarding the effect of the particle size, the obtained results showed that a mean size of 25 μm combined with a stirring speed of 200 rpm and working temperature of 80 °C was the most effective one. The optimized conditions showed a good adequacy with the Langmuir and Freundlich models. The discoloration process by using the type III activated carbon followed the pseudo-second-order kinetics.
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http://dx.doi.org/10.1021/acsomega.0c02717DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7450608PMC
August 2020

Aquatic toxicity and mode of action of CdS and ZnS nanoparticles in four microalgae species.

Environ Res 2020 07 14;186:109513. Epub 2020 Apr 14.

Far Eastern Federal University, Vladivostok, 690950, Russian Federation; N.I. Vavilov All-Russian Research Institute of Plant Genetic Resources, Saint Petersburg, 190121, Russian Federation; Pacific Geographical Institute FEB RAS, Vladivostok, 690014, Russian Federation.

This study reports the differences in toxic action between cadmium sulfide (CdS) and zinc sulfide (ZnS) nanoparticles (NPs) prepared by recently developed xanthate-mediated method. The aquatic toxicity of the synthesized NPs on four marine microalgae species was explored. Growth rate, esterase activity, membrane potential, and morphological changes of microalgae cells were evaluated using flow cytometry and optical microscopy. CdS and ZnS NPs demonstrated similar level of general toxicity and growth-rate inhibition to all used microalgae species, except the red algae P. purpureum. More specifically, CdS NPs caused higher inhibition of growth rate for C. muelleri and P. purpureum, while ZnS NPs were more toxic for A. ussuriensis and H. akashiwo species. Our findings suggest that the sensitivity of different microalgae species to CdS and ZnS NPs depends on the chemical composition of NPs and their ability to interact with the components of microalgal cell-wall. The red microalga was highly resistant to ZnS NPs most likely due to the presence of phycoerythrin proteins in the outer membrane bound Zn cations defending their cells from further toxic influence. The treatment with CdS NPs caused morphological changes and biochemical disorder in all tested microalgae species. The toxicity of CdS NPs is based on their higher photoactivity under visible light irradiation and lower dissociation in water, which allows them to generate more reactive oxygen species and create a higher risk of oxidative stress to aquatic organisms. The results of this study contribute to our understanding of the parameters affecting the aquatic toxicity of semiconductor NPs and provide a basis for further investigations.
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http://dx.doi.org/10.1016/j.envres.2020.109513DOI Listing
July 2020

The tellurite-reducing bacterium from a culture of the toxic dinoflagellate .

Heliyon 2019 Sep 25;5(9):e02435. Epub 2019 Sep 25.

A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, ul. Palchevskogo 17, Vladivostok, 690041, Russia.

The strain 2328 was isolated from a clonal culture of the toxic dinoflagellate . The strain exhibits a resistance to high KTeO concentrations (2500 μg/mL). A study of the growth dynamics of the strain exposed to KTeO has shown a longer lag phase and a reduced stationary phase compared to those during cultivation with no toxicant. The fatty acids profile is dominated by 16:1 (n-7), 16:0, 17:1, 15:0, 18:1 (n-7), and 17:0. The 2328 strain belongs to the Gammaproteobacteria and is related to the genus with 99-100% sequence similarity to some intra-genome allele variants (paralogues) of 16S rRNA from A phylogenetic reconstruction (ML and NJ), based on amino acid sequences, has revealed that the analyzed 2328 strain forms a common cluster with strains. In the presented work, the ability of to reduce potassium tellurite to elemental tellurium has been recorded for the first time. Bacteria reduce potassium tellurite to Te (0), nanoparticles of which become distributed diffusely and in the form of electron-dense globules in cytoplasm. Large polymorphous metalloid crystals are formed in the extracellular space. Such feature of the strain 2328 makes it quite attractive for biotechnological application as an organism concentrating the rare metalloid.
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http://dx.doi.org/10.1016/j.heliyon.2019.e02435DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6819836PMC
September 2019

Dependence of welding fume particle toxicity on electrode type and current intensity assessed by microalgae growth inhibition test.

Environ Res 2019 12 10;179(Pt B):108818. Epub 2019 Oct 10.

Far Eastern Federal University, Vladivostok, 690950, Russian Federation; Pacific Geographical Institute FEB RAS, 690014, Vladivostok, Russian Federation.

Welding fumes are a major source of metal oxide particles, ozone, carbon monoxide, carbon dioxide, nitrogen oxides, and many other toxic substances. Hazardous properties and the level of toxicity of welding fumes depend mostly on the welding electrode type and the welding regime parameters. The specific objective of this study was to evaluate the aquatic toxicity of metal welding fume particles in vivo on microalga Heterosigma akashiwo. The quantity and size of particles were measured by flow cytometry using a scattering laser light with a wavelength of 405 nm. The number of microalgae cells after 72 h and 7 days exposition with welding fume particle suspensions was evaluated by flow cytometry. Morphological changes of the microalga were observed by optical microscopy. The toxic effect was demonstrated as a significant reduction of cell density after exposure of microalgae to welding fume particles. The greatest impact on the growth of microalga was caused by particles with high rutile content. It was shown that the adverse effect of metal oxide particles depends more on the chemical composition of particles in welding fume while the number and dispersity of particles had no noticeable toxic influence on microalgae. The findings of this research confirm the fact that the toxicity of welding fume particles can be significantly reduced by using rutile-cellulose coated electrodes.
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http://dx.doi.org/10.1016/j.envres.2019.108818DOI Listing
December 2019

Accumulation and Tissue Distribution of Dinophysitoxin-1 and Dinophysitoxin-3 in the Mussel Crenomytilus grayanus Feeding on the Benthic Dinoflagellate Prorocentrum foraminosum.

Mar Drugs 2017 Oct 24;15(10). Epub 2017 Oct 24.

National Scientific Center of Marine Biology, A.V. Zhirmunsky Institute of Marine Biology, Far Eastern Branch of the Russian Academy of Sciences, ul. Palchevskogo 17, Vladivostok 690041, Russia.

A DTX-1-producing microalga, , from Peter the Great Bay, Sea of Japan, was fed to Gray's mussels, , for 12 days. An increase in DTX-1 and 7--acyl-DTX-1 (DTX-3) was observed in the digestive gland, kidneys, and gills. The digestive gland accumulated 91-100% of DTX-1 + DTX-3; and kidneys and gills accumulated, up to 8.5% and 4.3%, respectively. The kidneys had a distinctive pattern of toxin accumulation where the concentration of DTX-1 did not grow significantly after the eighth day of feeding, indicating the potential of DTX-1 elimination. The digestive gland and gills predominantly accumulated DTX-1, with a dramatic increase between Days 8 and 12. The DTX-3 content was highest in the digestive gland. The composition of DTX-3 in the acyl groups was similar for the digestive gland and kidneys, and did not change during feeding. The total toxin uptake of mussels exceeded the total toxin content from ingested cells by 2.4 times, showing that toxins may have accumulated from the seawater. This assumption needs to be further proved. The muscle, gonads, and mantle remained free of toxins. No genotoxic effect was observed in the gills and digestive gland.
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http://dx.doi.org/10.3390/md15100330DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5666436PMC
October 2017

Detection of Dinophysistoxin-1 in Clonal Culture of Marine Dinoflagellate Prorocentrum foraminosum (Faust M.A., 1993) from the Sea of Japan.

Toxins (Basel) 2015 Sep 28;7(10):3947-59. Epub 2015 Sep 28.

Zhirmunsky Institute of Marine Biology of the Far Eastern Branch of the Russian Academy of Sciences, Palchevskogo St., 17, Vladivostok 690041, Russia.

For the first time the presence of dinophysistoxin-1 (DTX-1) in a culture of Prorocentrum foraminosum was revealed in cells and in the culture medium. The clone was isolated from coastal waters of the Sea of Japan and identified by molecular analyses of SSU and D1/D2 regions of LSU rDNA. The concentration of DTX-1 in cells was 8.4 ± 2.5 pg/cell and, in cell-free media, 27.9 ± 14.7 µg/L. The toxin presence was confirmed by HPLC with high-resolution tandem mass-spectrometry.
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http://dx.doi.org/10.3390/toxins7103947DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4626713PMC
September 2015

Accelerated aging versus rejuvenation of the immune system in heterochronic parabiosis.

Rejuvenation Res 2012 Apr;15(2):239-48

Institute of Gerontology NAMS of Ukraine, Kyiv, Ukraine.

The emergence of immune disorders in aging is explained by many factors, including thymus dysfunction, decrease in the proportion and function of naïve T cells, and so forth. There are several approaches to preventing these changes, such as thymus rejuvenation, stem cells recovery, modulation of hormone production, and others. Our investigations of heterochronic parabiosis have shown that benefits of a young immune system, e.g., actively working thymus and regular migration of young hematopoietic stem cells between parabiotic partners, appeared unable to restore the immune system of the old partner. At the same time, we have established a progressive immune impairment in the young heterochronic partners. The mechanism of age changes in the immune system in this model, which may lead to reduced life expectancy, has not been fully understood. The first age-related manifestation in the young partners observed 3 weeks after the surgery was a dramatic increase of CD8(+)44(+) cells population in the spleen. A detailed analysis of further changes revealed a progressive decline of most immunological functions observable for up to 3 months after the surgery. This article reviews possible mechanisms of induction of age-related changes in the immune system of young heterochronic partners. The data obtained suggest the existence of certain factors in the old organisms that trigger aging, thus preventing the rejuvenation process.
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http://dx.doi.org/10.1089/rej.2012.1331DOI Listing
April 2012