Publications by authors named "Taoufiq Fechtali"

12 Publications

  • Page 1 of 1

Basil Essential Oil: Composition, Antimicrobial Properties, and Microencapsulation to Produce Active Chitosan Films for Food Packaging.

Foods 2021 Jan 8;10(1). Epub 2021 Jan 8.

Department of Agricultural Sciences, University of Naples Federico II, Via Università 100, 80055 Portici, Italy.

The essential oil (EO) from basil--was characterized, microencapsulated by vibration technology, and used to prepare a new type of packaging system designed to extend the food shelf life. The basil essential oil (BEO) chemical composition and antimicrobial activity were analyzed, as well as the morphological and biological properties of the derived BEO microcapsules (BEOMC). Analysis of BEO by gas chromatography demonstrated that the main component was linalool, whereas the study of its antimicrobial activity showed a significant inhibitory effect against all the microorganisms tested, mostly Gram-positive bacteria. Moreover, the prepared BEOMC showed a spheroidal shape and retained the EO antimicrobial activity. Finally, chitosan-based edible films were produced, grafted with BEOMC, and characterized for their physicochemical and biological properties. Since their effective antimicrobial activity was demonstrated, these films were tested as packaging system by wrapping cooked ham samples during 10 days of storage, with the aim of their possible use to extend the shelf life of the product. It was demonstrated that the obtained active film can both control the bacterial growth of the cooked ham and markedly inhibit the pH increase of the packaged food.
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http://dx.doi.org/10.3390/foods10010121DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7827191PMC
January 2021

Contrasting effects of Rhizophagus irregularis versus bacterial and fungal seed endophytes on Trifolium repens plant-soil feedback.

Mycorrhiza 2021 Jan 13;31(1):103-115. Epub 2020 Nov 13.

Department of Agricultural Sciences, University of Naples Federico II, via Università 100, 80055, Portici, NA, Italy.

Interactions between plants and soil affect plant-plant interactions and community composition by modifying soils conditions in plant-soil feedback, where associated microbes have the most crucial role. Both arbuscular mycorrhizal fungi (AMF) and microbial seed endophytes have been demonstrated to influence, directly or indirectly, biotic or abiotic soil properties, thus affecting subsequent plant growth, and community structure. However, little is known about how plant endophyte communities, individually or in interaction with AMF, affect plant-soil feedback processes. Here, we investigated, through a manipulative experiment, the behavior of endophyte-free and endophyte-associated Trifolium repens plants grown in soils previously conditioned by conspecific endophyte-free and endophyte-associated plants, inoculated or not by Rhizophagus intraradices. Furthermore, we identified microbial endophytes directly from the inner tissues of seeds by high-throughput sequencing, to compare seed fungal and bacterial endophyte composition. Results demonstrated that the outcome of simultaneous occurrence of seed endophytes and AMF on plant behavior depended on matching the endophytic status, i.e., either the presence or absence of seed microbial endophytes, of the conditioning and response phase. Seed fungal endophytes generated strong conspecific negative feedback, while seed bacterial endophytes proved to shift the feedback from negative to positive. Moreover, the simultaneous occurrence of both seed endophytes with AMF could either generate or expand negative plant-soil feedback effects. Our results show that seed and root symbionts can play a significant role on setting conspecific plant-soil feedback.
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http://dx.doi.org/10.1007/s00572-020-01003-4DOI Listing
January 2021

P-Solubilizing MS1B15 With Multiple Plant Growth-Promoting Traits Enhance Barley Development and Regulate Rhizosphere Microbial Population.

Front Plant Sci 2020 7;11:1137. Epub 2020 Aug 7.

Department of Agricultural Sciences, University of Naples Federico II, Naples, Italy.

Phosphate-solubilizing bacteria (PSB) have been reported to increase phosphate (P) content and plant growth. Their application in agricultural systems is an eco-friendly alternative strategy for limiting negative environmental impact of chemical fertilizers and increasing costs. Therefore, the aim of this study was to isolate and characterize new putative PSB to use as inoculum to enhance plant growth and increase P bioavailability in soil. Sixteen bacteria were isolated from Moroccan oat rhizosphere and were screened for their putative P-solubilization by semi-quantitative agar spot method. The two strains MS1B15 and MS1B13, identified as and , respectively, showed the maximum phosphate solubilization index (PSI = 1.75 and PSI = 1.63). After quantitative assay to determine phosphate solubilization activity, MS1B15 was selected for evaluating its putative plant growth promotion activities including production of siderophores, indole-3-acetic acid (IAA) and amino-cyclopropane-1-carboxylate (ACC) deaminase, nitrogen fixation and antimicrobial activity against soil-borne plant pathogens. Under greenhouse condition, barley plants inoculated with MS1B15 significantly increased shoot and ear length as well as available phosphorus in ears and leaves and P and N contents in the soil. Overall results showed that the selected strain MS1B15 could represent a potential candidate as biofertilizer to increase plant growth as well as P uptake.
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http://dx.doi.org/10.3389/fpls.2020.01137DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7426463PMC
August 2020

The cytotoxic activity of Salvia officinalis L. and Rosmarinus officinalis L. Leaves extracts on human glioblastoma cell line and their antioxidant effect.

J Complement Integr Med 2020 Mar 31. Epub 2020 Mar 31.

Laboratory of Biosciences, Functional, integrated and molecular exploration, School of Sciences and Technology- Mohammedia, Hassan II University of Casablanca, Casablanca, Morocco.

Background Rosmarinus officinalis L. (Rosemary) and Salvia officinalis L. (Sage) are two Mediterranean species growing spontaneously in some area in Morocco. They are used in traditional and complementary medicine to treat numerous disorders. The aim of this work was to assess the in vitro antitumoral effect of the methanolic total extract prepared from rosemary and sage on human glioblastoma cell line (42 GMBA), conjointly with their antioxidant activity. Methods The accelerated solvent extractor was used to obtain the total extract of the studied plants. The antitumor activity was performed using the microculture tetrazolium cytotoxique assay while the antioxidant effect was evaluated using the ferric reducing antioxidant power (FRAP), and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay. Results Our results show that the total extract of R.O and S.O have a cytotoxic effect on glioblastoma but not on cortical neurons. On the other hand, the results obtained in the FRAP and DPPH tests show a dose-dependent antioxidant activity correlated with an important level of phenols and flavonoids. Conclusion Rosmarinus officinalis L. and Salvia officinalis L. were found to have an antitumoral activity which may be linked, probably, to an antioxidant process.
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http://dx.doi.org/10.1515/jcim-2018-0189DOI Listing
March 2020

Chemical Composition and Antimicrobial Activity of Artemisia herba-alba and Origanum majorana Essential Oils from Morocco.

Molecules 2019 Nov 6;24(22). Epub 2019 Nov 6.

Laboratory of Biosciences, Integrated and Molecular Functional Exploration, Faculty of Sciences and Techniques Mohammedia, 146 Mohammedia 20650, Morocco.

Essential oils (EOs) are one of the most important groups of plant metabolites responsible for their biological activities. This study was carried out to study the chemical composition and the antimicrobial effects of Artemisia herba-alba and Origanum majorana essential oils against some Gram-positive and Gram-negative bacteria, and a fungal strain isolated from spoiled butter. The plants were collected in the region Azzemour of South West Morocco and the EOs, extracted by hydrodistillation, were analyzed by GC-MS. The antimicrobial activity was determined using the agar paper disc method. The main components of A. herba-alba EO were cis-thujone, trans-thujone and vanillyl alcohol; in O. majorana EO terpinen-4-ol, isopulegol and β-phellandrene predominated. Both essential oils exhibited growth inhibiting activities in a concentration-dependent manner on several microorganism species. Our results demonstrated that O. majorana and A. herba-alba EOs could be effective natural antibacterial agents in foods.
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http://dx.doi.org/10.3390/molecules24224021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6891654PMC
November 2019

Effect of Salvia Officinalis L. and Rosmarinus Officinalis L. leaves extracts on anxiety and neural activity.

Bioinformation 2019 15;15(3):172-178. Epub 2019 Mar 15.

Laboratory of Biosciences, Functional, integrated and molecular exploration, School Of Sciences and Technology - Mohammedia, Hassan II University of Casablanca.

Anxiety, the illness of our time, is one of the most prevalent and co-morbid psychiatric disorder that represents a significant socioeconomic burden. Conventional treatment is associated with a number of side effects and there is a need to develop new therapeutic strategies. Therefore, it is of interest to investigate the modulating effects of Salvia Officinalis L. and Rosmarinus Officinalis L. leaves extracts on anxiety using different behavioral tests, and on neural activity using the Multi-electrode array technique. Data shows the decrease of the time of the immobility associated with a significant increase in the time spent in the center of the open field arena in the treated animals compared to the controls. The number of buried marbles has also decreased in the treated animals in the marble-burying test. On the other hand results also show a decrease of the neural activity explained by a decrease of the number of spikes after 24,48 and 72 h following the addition of 12,5 µg/ml of the plant leaf extracts to the neural culture. However, there were no spikes after the administration of 25µg/ml of the plants extracts.
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http://dx.doi.org/10.6026/97320630015172DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6637401PMC
March 2019

Characterization of PI3KCA and BRAF mutations in gastric adenocarcinoma: An approach to a personalized targeted therapy for Moroccan HER2 overexpressed patients.

Arab J Gastroenterol 2018 Dec 28;19(4):155-158. Epub 2018 Nov 28.

Laboratory of Anatomy Pathology, Pasteur Institue, 20100, Morocco.

Background And Study Aims: Targeted therapies have an increasing importance in digestive oncology. To our knowledge, we are the first to report the distribution of PI3KCA and BRAF mutations in Moroccan HER2 overexpressed patients, in order to introduce targeted therapy in the arsenal of therapeutic modalities for management in Morocco.

Patients And Methods: 98 gastric adenocarcinoma tissue samples were collected. Further histological and immunohistochemical examinations were carried out at the Laboratory of Anatomy Pathology in Pasteur Institute-Morocco, in order to select HER2 positive cases. Out of 98 cases, 16 were found to be HER2-positive. The molecular study was performed for 55 good quality tissue samples including the HER2-positive ones, and activating mutations in H1047R PI3KCA and V600E BRAF were analyzed by Cast-PCR and Real-time PCR, respectively, at the Department of Molecular Biology, ANOUAL Specialized Center-Casablanca, Morocco. Statistical analyses were performed using the Epi-info software (version 6.09).

Results: Pi3KCA mutation was present in 8 cases (14,54%). BRAF mutation was present in 4 cases (7,27%) and 3 cases showed concomitant mutations. In total, 9 cases (16,36%) had PI3KCA and/or BRAF mutations.

Conclusion: The association between HER2 expression and PI3KCA alteration in gastric adenocarcinoma is most probably necessary to identify trastuzumab responders. Consequently, the 83,64% rate of HER2-positive patients harboring wild-type mutations possibly represents the portion of patients responding to trastuzumab while the 16,36% rate of patients carrying at least one of the studied mutations represents the portion of potentially non responsive patients to the targeted therapy, and thus may be considered as good candidates for multi-drug targeted therapy.
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http://dx.doi.org/10.1016/j.ajg.2018.08.001DOI Listing
December 2018

Functional Exploration Of T-Type Calcium Channels (Cav3.2 And Cav3.3) And Their Sensitivity To Zinc.

Open Microbiol J 2018 31;12:280-287. Epub 2018 Jul 31.

Department of Biology, Laboratory of Biosciences, Faculty of Sciences and Technology of Mohammedia, Casablanca, Morocco.

Introduction: T-type Ca channels (TTCC) are low Voltage-gated calcium channels, expressed in various tissues such as the brain and heart, and contribute to a variety of physiological functions including neuronal excitability, hormone secretion, muscle contraction, and pacemaker activity. At high concentrations, Zinc (Zn) is naturally attached to cell membranes and is therefore considered a reversible inhibitor of calcium. Zinc is also involved in the kinetics of sodium and potassium currents. Zinc is essential for many functions. A low zinc tenor is associated with emotional instability, digestive disorders, slow-growing and alteration of protein synthesis.

Material And Methods: For the Cell Culture we used HEK-293/tsA-201, and for transfection, the pCDNA3 plasmid constructs encoding human CaV3.2, and CaV3.3 subunits. Electrophysiological experiments were performed using the whole cell configuration of the patch-clamp technique. T-type currents were recorded using a test pulse from a holding potential at (-100mV) to (-30 mV), data Acquisition and Analysis for Current-voltage relationships (I-V curves) were recorded for the two cloned T-type Ca channels (Cav3.2, Cav3.3).

Results: Our studies describe the behavior of these channels Cav3.2 and Cav3.3 and also their current sensitivity to Zinc (Zn) in transfected HEK-293/tsA-201cells. Our results show that Zn applies a modulatory effect on T-type calcium channels. We observe that Zn differentially modulates the CaV3.2 and CaV3.3 channels. Zn preferably inhibits Cav3.2.

Conclusion: We have demonstrated that Zn differentially modulates two CaV3 channels (Cav3.2 and Cav3.3): It is a preferential blocker of CaV3.2 channels and it alters the gating behaviour of CaV3.3 channels.
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http://dx.doi.org/10.2174/1874285801812010280DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6110071PMC
July 2018

Modulation of T-type Ca2+ channels by Lavender and Rosemary extracts.

PLoS One 2017 26;12(10):e0186864. Epub 2017 Oct 26.

Institut de Génomique Fonctionnelle, CNRS, INSERM, University of Montpellier, Montpellier, France.

Medicinal plants represent a significant reservoir of unexplored substances for early-stage drug discovery. Of interest, two flowering Mediterranean plants have been used for thousands of years for their beneficial effects on nervous disorders, including anxiety and mood. However, the therapeutic potential of these plants regarding their ability to target ion channels and neuronal excitability remains largely unknown. Towards this goal, we have investigated the ability of Lavender and Rosemary to modulate T-type calcium channels (TTCCs). TTCCs play important roles in neuronal excitability, neuroprotection, sensory processes and sleep. These channels are also involved in epilepsy and pain. Using the whole-cell patch-clamp technique, we have characterized how Lavender and Rosemary extracts, as well as their major active compounds Linalool and Rosmarinic acid, modulate the electrophysiological properties of recombinant TTCCs (CaV3.2) expressed in HEK-293T cells. Both the methanolic and essential oil extracts as well as the active compounds of these plants inhibit Cav3.2 current in a concentration-dependent manner. In addition, these products also induce a negative shift of the steady-state inactivation of CaV3.2 current with no change in the activation properties. Taken together, our findings reveal that TTCCs are a molecular target of the Lavender and Rosemary compounds, suggesting that inhibition of TTCCs could contribute to the anxiolytic and the neuroprotective effects of these plants.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0186864PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5658086PMC
December 2017

Human Papillomavirus Genotyping and p16(INK4a) Expression in Cervical Lesions: A Combined Test to Avoid Cervical Cancer Progression.

J Cancer Prev 2016 Jun 30;21(2):121-5. Epub 2016 Jun 30.

Laboratory of Histo-Cytopathology of Institut Pasteur du Maroc, Hassan II University Casablanca, Casablanca, Morocco.

Cervical cancer is a major public health problem in Morocco. The cervical cancer has a long precancerous period that provides an opportunity for the screening and treatment. Improving screening tests is a priority goal for the early diagnosis of cervical cancer. This study was conducted to evaluate the combination of p16(INK4a) protein expression, human papillomavirus (HPV) typing, and histopathology for the identification of cervical lesions with high risk to progress to cervical cancer among Moroccan women. A total of 96 cervical biopsies were included in this study. Signal amplification in situ hybridization with biotinylated probes was used to detect HPV. Immunohistochemistry was used to evaluate the expression of p16(INK4a) protein. HPV DNA was detected in 74.0% of the biopsies (71/96). Of the seventy-one positive HPV cases, we detected 67.6% (48/71) of high risk (HR)-HPV (HPV 16 and 18), 24% of low risk-HPV (HPV 6 and 11), 1.4% intermediate risk-HPV (HPV 31, 33, and 35), and 7% coinfections (HPV 6/11 and 16/18). Overexpression of p16(INK4a) protein was observed in 72.9% (70/96) of the biopsies. In addition, p16(INK4a) protein detection was closely correlated with recovery of HR HPV. Our result showed that p16(INK4a) expression level is correlated with HR-HPV status.
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http://dx.doi.org/10.15430/JCP.2016.21.2.121DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4933437PMC
June 2016

Knowledge of Human Papillomavirus and Acceptability to Vaccinate in Adolescents and Young Adults of the Moroccan Population.

J Pediatr Adolesc Gynecol 2016 Jun 21;29(3):292-8. Epub 2015 Nov 21.

Laboratory of Neurosciences, Integrated Diseases and Natural Substances, Faculty of Sciences and Technics, Hassan II University, Mohammedia, Morocco.

Study Objective: Human papillomavirus (HPV) infection is estimated to play an etiologic role in 99.7% of cervical cancer. Vaccines can prevent up to 70% of the cervical cancer caused by HPV 16 and 18. The present study was designed to define the knowledge of HPV and HPV vaccine acceptability among Moroccan youth. DESIGN, SETTING, PARTICIPANTS, INTERVENTIONS, AND MAIN OUTCOME MEASURES: A nationwide anonymous questionnaire with a sample of 688 adolescents (12-17 years) and 356 young adults (18-30 years) was organized, that asked about HPV, origin of cervical cancer, Papanicolaou (Pap) test, and acceptability of HPV vaccine. Data were analyzed using univariate and multivariate logistic regression methods.

Results: Overall, a low frequency (213/1044 = 20%) of HPV knowledge was observed among the studied population. A multivariate model analysis showed that age, educational level, and knowledge of the Pap test remained significantly associated factors with HPV knowledge. Additionally, only 27% (282/1044) of participants were willing to accept HPV vaccination. Highest acceptability was observed among young adults compared with adolescents (166/356 = 46.6% vs 116/688 = 16.9%). Sixty-two percent (103/165) of male participants accepted the HPV vaccine compared with only 20.4% (179/879) of female participants. Educational level, type of school, and knowledge of the Pap test were associated factors with HPV vaccine acceptability in a multivariate model analysis.

Conclusion: The present study showed a low level of HPV knowledge and HPV vaccine acceptability among Moroccan youth. Promotion of activities and sensitization are required to maximize public awareness in the future. This objective can be achieved with the use of media, active efforts by health care providers, and introduction of sexual education in school programs.
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http://dx.doi.org/10.1016/j.jpag.2015.11.002DOI Listing
June 2016

RET genetic screening in patients with medullary thyroid cancer: the Moroccan experience.

J Cancer Res Ther 2009 Jul-Sep;5(3):198-202

Laboratoire d'Hormonologie et Marqueurs Tumoraux, Morocco.

Background: Germline RET gene mutations are well known to be the genetic causes of multiple endocrine neoplasia type 2 (MEN2) and may be identified by genetic screening.

Aim: The purpose of the present study was to screen nine MTC patients for RET sequence changes.

Materials And Methods: In this study, our sample was composed of 30 individuals: 9 index patients with medullary thyroid carcinoma (MTC) corresponding either to 3 subjects with clinical evidence of MEN2, 6 with apparently sporadic MTC (sMTC), and 21 relatives have been investigated for RET mutations. After DNA extraction from peripheral blood leukocytes, RET exons 8, 10, 11, 13-16 and exon/intron boundaries were analyzed by direct PCR sequencing.

Results: Three different known RET germline mutations in exon 11 (codon 634), p.Cys634Arg (c1900 T-->C) (de novo case), p.Cys634Phe (c1901 G-->T), p.Cys634Trp (c1902 C-->G), were detected in three individuals with MEN2 phenotype. Of the 21 relatives, 2 cases presented mutation. Among the six probands with sMTC, none was found to carry mutation. There was no difference between RET polymorphisms detected among both MEN2 and sMTC patients.

Conclusions: These preliminary data suggest that the RET mutation spectra observed in Moroccan patients with MEN2 are similar to those previously reported in other countries.
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http://dx.doi.org/10.4103/0973-1482.57126DOI Listing
June 2010
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