Publications by authors named "Tao Yu"

1,976 Publications

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Systematic Review and Meta-Analysis on the Rehabilitation Effect of Different Intensity Exercise on the Patients with Cardiovascular Diseases.

Comput Math Methods Med 2022 30;2022:1364985. Epub 2022 Jul 30.

First People's Hospital of Linping District, Hangzhou 311199, China.

Background: Cardiovascular disease is a common disease with high prevalence, disability, and mortality. Exercise therapy can improve cardiac functional reserve and life quality of patients, but the benefits of different exercise intensities for cardiovascular patients are still controversial. In this study, literature search and meta-analysis were used to explore the effect of 2 intensities of exercise on the rehabilitation effect of cardiovascular patients.

Methods: We searched Embase, Wiley online library, PubMed, Science Direct, and Clinicaltrials to look for randomized controlled trial (RCT) studies of high-intensity interval training (HIIT) versus moderate continuous training (MCT). After screening the inclusion criteria for the literature and assessing the risk of bias, a software analysis was performed using the R language toolkit to obtain forest plots and funnel maps.

Results: 10 articles were included in this study into the quantitative analysis, and 520 patients participated in the study; meta-analysis results showed that after HIIT intervention, the VO2 peak index of patients was higher than that of the MCT group (MD = 1.39, 95% CI (0.10, 2.68), = 2.12, = 0.0344), the peak heart rate HR peak was higher than that of the MCT training (MD = 7.71, 95% CI (5.12, 10.30), = 5.84, < 0.0001), the respiratory exchange rate (maximum RER) was higher than that of the MCT training (MD = 0.02, 95% CI (0.00, 0.04), = 2.36, = 0.0184), and the quality of life was higher than that of the MCT training (MD = 0.39, 95% CI (0.07, 0.71), = 2.40, = 0.0165). . Compared with moderate continuous training, high intensity interval training is more conducive to improve the cardiopulmonary function of cardiovascular patients and improve their physical life quality.
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http://dx.doi.org/10.1155/2022/1364985DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356822PMC
August 2022

-Arylimidazoliums as Highly Selective Biomimetic Antimicrobial Agents.

J Med Chem 2022 Aug 5. Epub 2022 Aug 5.

National Engineering Research Center for Biomaterials, Sichuan University, 29 Wangjiang Road, Chengdu 610064, China.

Antibiotic resistance has become one of the greatest health threats in the world. In this study, a charge-dispersed dimerization strategy is described for the antimicrobial peptide (AMP) mimics via a tunable cationic charge to improve the selectivity between prokaryotic microbes and eukaryotic cells. This strategy is demonstrated with a series of charge-dispersed AMP mimics based on -arylimidazolium skeletons. These -arylimidazolium AMP mimics show potent antibacterial activity against strains along with a low rate of drug resistance, good hemocompatibility, and low cytotoxicity. In addition to the elimination of planktonic bacteria, -arylimidazolium AMP mimics can also inhibit biofilm formation and destroy the established biofilm. More importantly, methicillin-resistant (MRSA)-induced lung-infected mice can be effectively treated by the intravenous administration of -arylimidazolium AMP mimic, which enable the design of -arylimidazolium AMP mimics to offer an alternative avenue to eradicate drug-resistant bacterial infection.
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http://dx.doi.org/10.1021/acs.jmedchem.2c00818DOI Listing
August 2022

Recent advances in targeted delivery of non-coding RNA-based therapeutics for atherosclerosis.

Mol Ther 2022 Aug 1. Epub 2022 Aug 1.

Institute for translational medicine, The Affiliated Hospital of Qingdao University, No. 38 Dengzhou Road, 266021, People's Republic of China; Department of Cardiac Ultrasound, The Affiliated Hospital of Qingdao University, Qingdao 266000, China;. Electronic address:

Cardiovascular disease (CVD) has overtaken infectious illnesses as the leading cause of mortality and disability worldwide. The pathology that underpins CVD is atherosclerosis, characterized by chronic inflammation caused by the accumulation of plaques in the arteries. As our knowledge about the microenvironment of blood vessel walls deepens, there is an opportunity to fine-tune treatments to target the mechanisms driving atherosclerosis more directly. The application of non-coding RNAs (ncRNAs) as biomarkers or intervention targets is increasing. Although these ncRNAs play an important role in driving atherosclerosis and vascular dysfunction, the cellular and extracellular environment pose a challenge for targeted transmission and therapeutic regulation of ncRNAs. Specificity, delivery, and tolerance have hampered the clinical translation of ncRNA-based therapeutics. Nanomedicine is an emerging field that uses nanotechnology for targeted drug delivery and advanced imaging. Recently, nanoscale carriers have shown promising results and introduced new possibilities for nucleic acid targeted drug delivery, particularly for atherosclerosis. In this review, we discuss the latest developments in nanoparticles to aid ncRNA-based drug development, particularly miRNA, and analyze the current challenges in ncRNA targeted delivery. In particular, we highlight the emergence of various kinds of nanotherapeutic approaches based on ncRNAs, which can improve treatment options for atherosclerosis.
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http://dx.doi.org/10.1016/j.ymthe.2022.07.018DOI Listing
August 2022

Golgi stress induces SIRT2 to counteract Shigella infection via defatty-acylation.

Nat Commun 2022 Aug 2;13(1):4494. Epub 2022 Aug 2.

Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY, 14853, USA.

Enzymes from pathogens often modulate host protein post-translational modifications (PTMs), facilitating survival and proliferation of pathogens. Shigella virulence factors IpaJ and IcsB induce proteolytic cleavage and lysine fatty acylation on host proteins, which cause Golgi stress and suppress innate immunity, respectively. However, it is unknown whether host enzymes could reverse such modifications introduced by pathogens' virulence factors to suppress pathogenesis. Herein, we report that SIRT2, a potent lysine defatty-acylase, is upregulated by the transcription factor CREB3 under Golgi stress induced by Shigella infection. SIRT2 in turn removes the lysine fatty acylation introduced by Shigella virulence factor IcsB to enhance host innate immunity. SIRT2 knockout mice are more susceptible to Shigella infection than wildtype mice, demonstrating the importance of SIRT2 to counteract Shigella infection.
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http://dx.doi.org/10.1038/s41467-022-32227-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9345896PMC
August 2022

Controllable Free Viewpoint Video Reconstruction Based on Neural Radiance Fields and Motion Graphs.

IEEE Trans Vis Comput Graph 2022 Aug 1;PP. Epub 2022 Aug 1.

In this paper, we propose a controllable high-quality free viewpoint video generation method based on the motion graph and neural radiance fields (NeRF). Different from existing pose-driven NeRF or time/structure conditioned NeRF works, we propose to first construct a directed motion graph of the captured sequence. Such a sequence-motion-parameterization strategy not only enables flexible pose control for free viewpoint video rendering but also avoids redundant calculation of similar poses and thus improves the overall reconstruction efficiency. Moreover, to support body shape control without losing the realistic free viewpoint rendering performance, we improve the vanilla NeRF by combining explicit surface deformation and implicit neural scene representations. Specifically, we train a local surface-guided NeRF for each valid frame on the motion graph, and the volumetric rendering was only performed in the local space around the real surface, thus enabling plausible shape control ability. As far as we know, our method is the first method that supports both realistic free viewpoint video reconstruction and motion graph-based user-guided motion traversal. The results and comparisons further demonstrate the effectiveness of the proposed method.
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http://dx.doi.org/10.1109/TVCG.2022.3192713DOI Listing
August 2022

Identification of key pathways and genes in the progression of silicosis based on WGCNA.

Inhal Toxicol 2022 Aug 1:1-15. Epub 2022 Aug 1.

Department of Toxicology, Key Lab of Chemical Safety and health, National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing, China.

Silicosis, induced by inhaling silica particles in workplaces, is one of the most common occupational diseases. The prognosis of silicosis and its consequent fibrosis is extremely poor due to limited treatment modalities and lack of understanding of the disease mechanisms. In this study, a Wistar rat model for silicosis fibrosis was established by intratracheal instillation of silica (0, 50, 100 and 200 mg/mL, 1 mL) with the evidence of Hematoxylin and Eosin (HE) and Masson staining and the expressions of inflammatory and fibrotic proteins of rats' lung tissues. RNA of lung tissues of rats exposed to 200 mg/mL silica particles and normal saline for 14 d and 28 d was extracted and sequenced to detect differentially expressed genes (DEGs) and to identify silicosis fibrosis-associated modules and hub genes by Weighted gene co-expression network analysis (WGCNA). Predictions of gene functions and signaling pathways were conducted using Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. In this study, it has been demonstrated the promising role of the Hippo signaling pathway in silicosis fibrosis, which will be conducive to elucidating the specific mechanism of pulmonary fibrosis induced by silica and to determining molecular initiating event (MIE) and adverse outcome pathway (AOP) of silicosis fibrosis.
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http://dx.doi.org/10.1080/08958378.2022.2102700DOI Listing
August 2022

Fiber-bundle-based 2D Raman and Rayleigh imaging for major species and temperature measurement in laminar flames.

Opt Lett 2022 Aug;47(15):3764-3767

In this Letter, we introduce a novel fiber-bundle-based 2D Raman and Rayleigh imaging system for 2D measurements of major species mole fractions and temperature in flames. A cascade of dichroic mirrors and bandpass filters separate the Raman signal associated with each major species and direct it to a custom-built four-inputs/one-output fiber bundle. The single output of the fiber bundle allows simultaneous detection of four species by using a single back-illuminated CCD camera. The novel imaging system combined with a pulse-burst laser operated at 10 kHz and a 10 kHz optical shutter is characterized from measurements in a Mckenna burner and then applied to a flame stabilized over a Santoro burner. The work is, to the best of our knowledge, the first demonstration of 2D measurements of temperature and major species (i.e., N, O, H, and HO) in H-air diffusion flames at atmospheric pressure.
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http://dx.doi.org/10.1364/OL.467713DOI Listing
August 2022

Revolution of CAR Engineering For Next-Generation Immunotherapy In Solid Tumors.

Front Immunol 2022 12;13:936496. Epub 2022 Jul 12.

Suzhou Cancer Center Core Laboratory, Suzhou Municipal Hospital, Gusu School, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, China.

Chimeric antigen receptor (CAR)-T cells have enormous potentials for clinical therapies. The CAR-T therapy has been approved for treating hematological malignancies. However, their application is limited in solid tumors owing to antigen loss and mutation, physical barriers, and an immunosuppressive tumor microenvironment. To overcome the challenges of CAR-T, increasing efforts are put into developing CAR-T to expand its applied ranges. Varied receptors are utilized for recognizing tumor-associated antigens and relieving immunosuppression. Emerging co-stimulatory signaling is employed for CAR-T activation. Furthermore, other immune cells such as NK cells and macrophages have manifested potential for delivering CAR. Hence, we collected and summarized the last advancements of CAR engineering from three aspects, namely, the ectodomains, endogenous domains, and immune cells, aiming to inspire the design of next-generation adoptive immunotherapy for treating solid tumors.
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http://dx.doi.org/10.3389/fimmu.2022.936496DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315443PMC
August 2022

Potential Role of Pain Catastrophic Thinking in Comorbidity Patients of Depression and Chronic Pain.

Front Psychiatry 2022 8;13:839173. Epub 2022 Jul 8.

Anhui Clinical Research Center for Mental Disorders, Affiliated Psychological Hospital of Anhui Medical University, Hefei, China.

Background: Although comorbidity of major depressive disorder (MDD) and chronic pain (CP) has been well-studied, their association with pain catastrophizing is largely elusive. This study aimed to investigate the potential effects of pain catastrophizing in patients with a comorbidity.

Methods: In total, 140 participants were included in this study and divided into three groups according to the Diagnostic and Statistical Manual of Mental Disorders and the International Association for the study of pain (i.e., the comorbidity group: patients with depression with chronic pain, = 45; depression group: patients with depression without chronic pain, = 47; and healthy controls: = 48). The Hamilton Depression Rating Scale (HAMD)-24 and Hamilton Anxiety Rating Scale (HAMA)-14 were used by professional psychiatrists to evaluate the severity of depression and anxiety. Beck Depression Inventory-II (BDI-II) and Beck Anxiety Inventory (BAI) were conducted by patients' self-report to assess the symptom severity. The pain intensity numerical rating scale (PI-NRS) was used to assess the pain intensity. Pain Catastrophizing Scale (PCS) and Pain Anxiety Symptoms Scale (PASS) were used to estimate pain-related negative thinking.

Results: The results showed that PASS and PCS scores were significantly different among the three groups. Particularly, the scores in the comorbidity group were the highest. The Pearson correlation analysis revealed a positive correlation between PCS (including the patients' helplessness, magnification, rumination, and total scores) and the severity of depression symptoms, anxiety symptoms, and pain intensity ( < 0.05). A stepwise regression analysis further demonstrated that the total PCS score, high monthly income level, and BDI score had positive impacts on PASS ( < 0.05). We also found that the total BDI score, disease course ≥1 year, and pain intensity had positive effects on PCS ( < 0.05), whereas years of education (≤ 12 years) had a negative effect on PCS ( = 0.012). In all, we have clearly demonstrated that PCS and PASS could serve as potentially predictive factors in patients suffering from comorbidity of MDD and CP.

Conclusion: Our results suggested that the pain-related catastrophic thinking and anxiety were more severe in the comorbidity group than in MDD-only group and healthy group. Pain-related catastrophizing thoughts and anxiety may have potentially effects on the comorbidity of depression and chronic pain.
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http://dx.doi.org/10.3389/fpsyt.2022.839173DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309267PMC
July 2022

MEC/Cloud Orchestrator to Facilitate Private/Local Beyond 5G with MEC and Proof-of-Concept Implementation.

Sensors (Basel) 2022 Jul 8;22(14). Epub 2022 Jul 8.

Rakuten Mobile, Inc., Tokyo 151-0051, Japan.

The emergence of 5G-IoT opens up unprecedented connectivity possibilities for new service use cases and players. Multi-access edge computing (MEC) is a crucial technology and enabler for Beyond 5G, supporting next-generation communications with service guarantees (e.g., ultra-low latency, high security) from an end-to-end (E2E) perspective. On the other hand, one notable advance is the platform that supports virtualization from RAN to applications. Deploying Radio Access Networks (RAN) and MEC, including third-party applications on virtualization platforms, and renting other equipment from legacy telecom operators will make it easier for new telecom operators, called Private/Local Telecom Operators, to join the ecosystem. Our preliminary studies have discussed the ecosystem for private and local telecom operators regarding business potential and revenue and provided numerical results. What remains is how Private/Local Telecom Operators can manage and deploy their MEC applications. In this paper, we designed the architecture for fully virtualized MEC 5G cellular networks with local use cases (e.g., stadiums, campuses). We propose an MEC/Cloud Orchestrator implementation for intelligent deployment selection. In addition, we provide implementation schemes in several cases held by either existing cloud owners or private and local operators. In order to verify the proposal's feasibility, we designed the system level in E2E and constructed a Beyond 5G testbed at the Ōokayama Campus of the Tokyo Institute of Technology. Through proof-of-concept in the outdoor field, the proposed system's feasibility is verified by E2E performance evaluation. The verification results prove that the proposed approach can reduce latency and provide a more stable throughput than conventional cloud services.
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http://dx.doi.org/10.3390/s22145145DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9325042PMC
July 2022

Relationship between Dietary Patterns and Subjectively Measured Physical Activity in Japanese Individuals 85 Years and Older: A Cross-Sectional Study.

Nutrients 2022 Jul 17;14(14). Epub 2022 Jul 17.

Center for Supercentenarian Medical Research, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.

High-quality diets and regular physical activity (PA) are considered healthy behaviors (HBs). HBs are associated with many health outcomes and are expected to improve quality of life. Although implementing HBs is important, the relationship between dietary patterns (DPs) and PA has not been well investigated, especially among those aged ≥ 85. This study used data from the Tokyo Oldest Old survey on Total Health study to examine the relationship between DPs and PA in a cross-sectional study. The dietary survey used the brief self-administered diet history questionnaire to estimate the intake of 58 foods. After energy adjustment, principal component analysis was performed to identify major DPs. A validated questionnaire was used to evaluate PA, and linear regression analysis was used to investigate the association between DPs and PA, considering confounders. A total of 519 participants were included. Three major DPs ('Various plant foods', 'Fish and mushrooms', 'Cooked rice and miso soup') were identified. 'Various plant foods' was similar to DPs previously named 'Healthy' or 'Prudent', and its trend was positively associated with higher PA. This study observed the implementation of HBs even among those aged ≥ 85, suggesting that a trend toward a healthier diet is associated with higher PA.
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http://dx.doi.org/10.3390/nu14142924DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323235PMC
July 2022

Comparative Transcriptome and Interaction Protein Analysis Reveals the Mechanism of -Overexpressing Transgenic Sweet Potato Response to Low-Temperature Stress.

Genes (Basel) 2022 Jul 14;13(7). Epub 2022 Jul 14.

Xuzhou Sweet Potato Research Center, Xuzhou Institute of Agricultural Sciences Jiangsu, China/Key Laboratory of Sweet Potato Biology and Genetic Breeding, Ministry of Agriculture/National Agricultural Experimental Station for Soil Quality, Xuzhou 221000, China.

The sweet potato is very sensitive to low temperature. Our previous study revealed that -overexpressing transgenic sweet potato (M3) plants showed stronger low-temperature stress tolerance than wild-type plants (WT). However, the mechanism of M3 plants in response to low-temperature stress is unclear. To further analyze how mediates low-temperature stress in sweet potato, WT and M3 plants were exposed to low-temperature stress for 2 h and 12 h for RNA-seq analysis, whereas normal conditions were used as a control (CK). In total, 3436 and 8718 differentially expressed genes (DEGs) were identified in WT at 2 h (vs. CK) and 12 h (vs. CK) under low-temperature stress, respectively, whereas 1450 and 9291 DEGs were detected in M3 plants, respectively. Many common and unique DEGs were analyzed in WT and M3 plants. DEGs related to low temperature were involved in Ca signaling, MAPK cascades, the reactive oxygen species (ROS) signaling pathway, hormone transduction pathway, encoding transcription factor families (, , and ), and downstream stress-related genes. Additionally, more upregulated genes were associated with the MAPK pathway in M3 plants during short-term low-temperature stress (CK vs. 2 h), and more upregulated genes were involved in secondary metabolic synthesis in M3 plants than in the WT during the long-time low-temperature stress treatment (CK vs. 12 h), such as fatty acid biosynthesis and elongation, glutathione metabolism, flavonoid biosynthesis, carotenoid biosynthesis, and zeatin biosynthesis. Moreover, the interaction proteins of IbMPK3 related to photosynthesis, or encoding CaM, NAC, and ribosomal proteins, were identified using yeast two-hybrid (Y2H). This study may provide a valuable resource for elucidating the sweet potato low-temperature stress resistance mechanism, as well as data to support molecular-assisted breeding with the gene.
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http://dx.doi.org/10.3390/genes13071247DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317282PMC
July 2022

Relationship between Serum miR-106 and MYL4 Levels and the Prevalence, Risk Stratification, and Prognosis of Atrial Fibrillation.

J Immunol Res 2022 14;2022:1069866. Epub 2022 Jul 14.

Department of Internal Medicine-Cardiovascular, Jiangxi Provincial People's Hospital, The First Affiliated Hospital of Nanchang Medical College, Nanchang 330000, Jiangxi Province, China.

Objective: To analyze the predictive value of serum microRNA-106 (miRNA-106), miR-106, and myosin light chain 4 (MYL4) levels on the prevalence of atrial fibrillation and to explore the relationship between serum miR-106 and MYL4 and the risk stratification and prognosis of atrial fibrillation, thereby providing basis for them to become clinical targets for the treatment of atrial fibrillation in the future.

Methods: 300 patients with atrial fibrillation treated in our hospital from May 2017 to March 2019 were selected as the atrial fibrillation group, and 300 healthy people who came to our hospital for physical examination in the same period were selected as the control group. The general data of the subjects in the two groups were collected. The serum miR-106 level of the subjects in the two groups was detected by fluorescence quantitative polymerase chain reaction (PCR), and the level of MYL4 was detected by enzyme-linked immunosorbent assay (ELISA). The expression of miR-106 and MYL4 in the myocardium was observed by immunohistochemistry. The relationship between the levels of serum miR-106 and MYL4 and the prevalence of atrial fibrillation and the score of atrial fibrillation thromboembolism risk stratification scoring system (cha2ds2) was compared between the two groups. The relationship between serum level of miR-106 and prognosis of patients with atrial fibrillation was analyzed.

Results: The systolic blood pressure, diastolic blood pressure, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and left anterior descending artery (LAD) in the atrial fibrillation group were significantly higher than those in the control group, while HDL-C and left ventricular ejection fraction (LVEF) were significantly lower than those in the control group ( < 0.01). The level of serum miR-106 in patients with atrial fibrillation was significantly higher than that in the control group, whereas the level of MYL4 was significantly lower than that in the control group ( < 0.01). miR-106 was mainly localized in the cytoplasm, and the positive expression rate of miR-106 was 71.43% (81/115) in patients with atrial fibrillation and 21.74% (25/115) in patients with sinus rhythm. MYL4 was mainly located in the cell membrane and the positive expression rate of MYL4 was 24.35% (28/115) in patients with atrial fibrillation and 64.35% (74/115) in patients with sinus rhythm. With the increase of the severity of atrial fibrillation, the level of serum miR-106 gradually increased and the level of MYL4 gradually decreased, which were statistically significant compared with the control group ( < 0.05). With the increase of miR-106 level and the decrease of MYL4 level, the prevalence of atrial fibrillation gradually increased. With the increase of cha2ds2 score, the level of serum miR-106 increased and the level of MYL4 decreased. The survival rate of patients with miR - 106 ≤ 1.96 was significantly higher than that of patients with miR - 106 > 1.96. The survival rate of patients with MYL4 ≥ 0.24 was significantly higher than that of patients with MYL4 < 0.24. At the same time, TC and LDL-C were included in the analysis. The results showed that the survival rate of patients with TC ≤ 4.5 mmol/L was significantly higher than that of patients with TC > 4.5 mmol/L, and that of patients with LDL-C ≤ 2.6 mmol/L was significantly higher than that of patients with LDL-C > 2.6 mmol/L.

Conclusion: Serum miR-106 and MYL4 levels are closely related to the prevalence of atrial fibrillation, which can reflect the risk of thromboembolism in patients with atrial fibrillation and can be used as a biological indicator to predict the prognosis of patients with atrial fibrillation.
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http://dx.doi.org/10.1155/2022/1069866DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9303158PMC
July 2022

Bovine serum albumin-gold nanoclusters protein corona stabilized polystyrene nanoparticles as dual-color fluorescent nanoprobes for breast cancer detection.

Biosens Bioelectron 2022 Jul 16;215:114575. Epub 2022 Jul 16.

Laboratory of Biomaterials and Translational Medicine, Center for Nanomedicine, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510630, China. Electronic address:

Breast cancer is the most prevalent malignancy and the first leading cause of cancer-related mortality among the female population worldwide. Approaches for precise and reliable detection of breast cancer cells, particularly in the nascent state, are desperately needed for elevating the survival rate of patients bearing the breast tumor. In this work, we successfully performed the sensitive, precise, and reliable breast cancer cell detection using facilely fabricated bovine serum albumin-gold nanocluster (BSA-AuNCs) protein corona stabilized, epithelial cell adhesion molecule (EpCAM) aptamer linked fluorescent polystyrene nanoparticle (PS NP), termed as PS-BSA-AuNCs-Apt. The rapidly adsorbed BSA-AuNCs hard protein corona without complicated covalent conjugation not only imparted excellent colloidal stability to the PS nanoparticles, but also offered numerous active anchors for the targeted EpCAM aptamers to locate. With the remarkable aid of the aptamers specifically targeting the EpCAM-positive breast cancer cells, the PS-BSA-AuNCs-Apt emitted strong and photostable dual-color fluorescent signals for precise and reliable cancer cell detection by overcoming the false signals. The specific identification potency of the PS-BSA-AuNCs-Apt system was further verified by successfully detecting the xenografted breast tumor tissue. Notably, to the best of our knowledge, the protein corona formed nanoprobes was exploited for direct tumor cell and tissue detection with high efficacy for the first time, demonstrating their promising potential in clinical tumor detection.
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http://dx.doi.org/10.1016/j.bios.2022.114575DOI Listing
July 2022

Insights into Pyrroloquinoline Quinone (PQQ) Effects on Soil Nutrients and Pathogens from Pepper Monocropping Soil under Anaerobic and Aerobic Conditions.

Microbiol Spectr 2022 Jul 19:e0093322. Epub 2022 Jul 19.

Hunan Academy of Agricultural Sciences, Changsha, Hunan, China.

Imbalances of soil available nutrients and soilborne diseases have seriously restricted the productivity of crops and jeopardized food security worldwide. Pyrroloquinoline quinone (PQQ), a redox cofactor in some bacteria involved in glucose metabolism and phosphorus mineralization, could be anticipated to alter soil ecosystems to a certain extent. However, there is limited information on PQQ defending soilborne pathogens and regulating soil main nutrients. Here, a pot experiment based on mono-cropping soils of pepper was conducted to examine the effects of PQQ amendment on reconstructing soil microbial communities and soil nutrients under aerobic/anaerobic conditions comprising three treatments, namely, control, PQQ (aerobic), and FL-PQQ (anaerobic). The results revealed that soil microbial community composition and soil nutrients were distinctly altered by PQQ regimes. Compared to control, PQQ treatment significantly increased the content of soil available phosphorus (AP), while FL_PQQ treatment strongly improved the content of soil available nitrogen (AN). In terms of pathogens, relative to control, both PQQ treatments suppressed the abundances of pathogens, of which FL_PQQ treatment significantly decreased the abundance of the pathotrophic fungal by 64% and the abundance of Fusarium oxysporum by 57%, largely attributed to the increase of organic acid generators (O, ) and potential antagonists (). Structural equation modeling (SEM) showed that PQQ regimes suppressed pathogens by indirectly regulating soil physicochemical properties and microbial communities. Overall, we proposed that PQQ application both in aerobic/anaerobic conditions could improve soil available nutrients and suppress soil pathogens in pepper monocropping soils. The attention to PQQ (pyrroloquinoline quinone) effect on soil nutrients and pathogens was less paid in monocropping soils. However, the underlying microbial interacting mechanism remains unclear. Adopting a novel external bio-additive, the effects of PQQ on soil main nutrients and the pathotrophic fungal under aerobic and anaerobic regimes will be investigated, which would help to improve soil quality health. Our main conclusion was that PQQ would help to remediate monocropping obstacle soils in terms of soil nutrients and soil pathogens by associating with the microbial community, and anaerobic PQQ application more favored amelioration of continuous obstacle soils. These results will benefit the health and sustainable development of pepper production as well as other greenhouse vegetable production.
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http://dx.doi.org/10.1128/spectrum.00933-22DOI Listing
July 2022

Ketone Body Improves Neurological Outcomes after Cardiac Arrest by Inhibiting Mitochondrial Fission in Rats.

Oxid Med Cell Longev 2022 7;2022:7736416. Epub 2022 Jul 7.

Department of Emergency Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China.

Ketone bodies including -hydroxybutyrate (-HB) have been proved the therapeutic potential in diverse neurological disorders. However, the role of -HB in the regulation of neurological injury after cardiac arrest (CA) remains unclear. We investigated the effect of -HB on brain mitochondrial dysfunction and neurological function after CA. A rat model of CA was established by asphyxia. The rats were randomly divided into three groups: sham group, control group, and -HB group. Animals received 200 mg/kg -HB or same volume vehicle at 10 minutes after return of spontaneous circulation by intraperitoneal injection. Neurological function was evaluated by neurologic deficit score and Y-maze. Neuronal cell loss and apoptosis were detected through hematoxylin-eosin staining, Nissl staining, and TdT-mediated dUTP nick-end labeling assay. Oxidative stress levels were determined by immunohistochemical staining of 4-hydoxynonenal and 8-hydroxy-2'-deoxyguanosine. Furthermore, mitochondrial ultrastructure of brain cells was observed by transmission electron microscopy. In addition, the protein expression levels of Bak, caspase 3, gasdermin D, caspase 1, brain-derived neurotrophic factor, dynamin-related protein 1 (Drp1), and phospho-Drp1 (ser616) were measured. We found that neurological function and survival rate were significantly higher in the -HB group compared with the control group. -HB also reduced neurons death and neurological oxidative stress after CA. Moreover, -HB reduced neurological injury from apoptosis and pyroptosis after CA. In addition, -HB maintained the structural integrity of brain mitochondria, prevented mitochondrial fission, and increased brain energy metabolism after CA. In conclusion, -HB beneficially affected the neurological function of rats after global cerebral ischemia, associated with decreased mitochondrial fission, and improved mitochondrial function. Our results suggest that -HB might benefit patients suffering from neurological dysfunction after CA.
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http://dx.doi.org/10.1155/2022/7736416DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9283010PMC
July 2022

Injectable electrospun fiber-hydrogel composite sequentially releasing clonidine and ropivacaine for prolonged and walking regional analgesia.

Theranostics 2022 21;12(11):4904-4921. Epub 2022 Jun 21.

Department of Anesthesiology and Center for Nanomedicine, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China.

: Peripheral nerve block is a traditional perioperative analgesic method for its precise pain control and low systemic toxicity. However, a single low dose of local anesthetic merely provides a few hours of analgesia, and high dose results in irreversible toxicity, whereas continuous infusion of anesthetics is expensive and complicated. Therefore, it is necessary to develop a long-acting and sensory-selective local anesthetic for safe perioperative analgesia. : An injectable composite comprising ropivacaine-loaded poly (ε-caprolactone) electrospun fiber and clonidine-loaded F127 hydrogel (Fiber-Rop/Gel-Clo composite) was developed for long-acting and walking regional analgesia with barely one dose. The peripheral nerve blockade effect of the composite was evaluated in a rat sciatic nerve block model. Also, the biodegradability and biosafety of the composite was evaluated. : The preferentially released Clo from the hydrogel rapidly constricted the peripheral arterial vessels, reducing the blood absorption of Rop and thus enhancing the local Rop accumulation at the injection site. The subsequently sustainable release of Rop from the fiber, significantly prolonged the sciatic nerve block of rats. Remarkably, an amazing sensorimotor segregation effect was achieved, as the sensory blockade (32.0 ± 1.4 h) lasted significantly longer than the motor blockade (20.3 ± 0.9 h). Additionally, the Fiber-Rop/Gel-Clo composite presented good biodegradability and biosafety . : Our designed Fiber-Rop/Gel-Clo composite with minimal invasion, prolonged synergistic analgesia, and strikingly sensorimotor segregation effect, posted a promising prospect for regional long-term walking analgesia in clinical treatment.
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http://dx.doi.org/10.7150/thno.74845DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9274753PMC
June 2022

Leaching experiments and risk assessment to explore the migration and risk of potentially toxic elements in soil from black shale.

Sci Total Environ 2022 Jul 5;844:156922. Epub 2022 Jul 5.

Key Laboratory of Ecogeochemistry, Ministry of Natural Resources, Beijing 100037, PR China; School of Earth Sciences and Resources, China University of Geosciences, Beijing 100083, PR China.

Black shale is rich in potentially toxic elements (PTEs) that migrate through rock weathering or rainfall, adversely affecting human health and the environment. In this study, simulated rainfall leaching experiments were used to investigate the migration patterns and leaching kinetics of PTEs in black shale from the Lower Cambrian Hetang Formation and to analyze the water quality index (WQI) of PTEs in the leachate. A comparison between the risk of PTEs in the leachate and those in the soil was also made to determine the risk sources, risk status, and distribution characteristics of PTEs in the study area. The WQI of the indoor column experimental leachate indicated the highest As contamination. The geo-accumulation index (I) and potential ecological risk (Er) of soils in the entire region revealed that the risk of Cd was the highest. Furthermore, by mapping the distribution of I and Er in soils, the risk level in the region where black shale is located was found to be significantly higher than that in other areas. Comparing the leaching rate of PTEs with the WQI from leaching experiments, the risk associated with As in soil can be inferred to originate mainly from the leaching of black shale. Previous studies on PTEs in black shale in the study area tended to focus on Cd; however, this study found that the risk of As was not negligible. The health risk assessment also showed that the risk at the location of black shale was beyond the accepted range. Overall, this study provided a new and important evaluation law for the level of pollution by PTEs and health risks in typical black shale regions.
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http://dx.doi.org/10.1016/j.scitotenv.2022.156922DOI Listing
July 2022

Modulation of Microglia M2 Polarization and Alleviation of Hippocampal Neuron Injury By MiR-106b-5p/RGMa in a Mouse Model of Status Epilepticus.

Inflammation 2022 Jul 4. Epub 2022 Jul 4.

Department of Pediatrics, Shengjing Hospital of China Medical University, Liaoning Province, Shenyang City, 110004, China.

MicroRNAs (miRNAs) regulate gene expression at the post-transcriptional level. The miRNA miR-106b-5p has been linked to epilepsy, but its specific role and mechanism of action remain unclear. This was investigated in the present study using a mouse model of pilocarpine-induced status epilepticus and an in vitro system of HT22 hippocampal cells treated with Mg-free solution and cocultured with BV2 microglia cells. We found that inhibiting miR-106b-5p expression promoted microglia M2 polarization, reduced the inflammatory response, and alleviated neuronal injury. These effects involved modulation of the repulsive guidance molecule A (RGMa)-Rac1-c-Jun N-terminal kinase (JNK)/p38-mitogen-activated protein kinase (MAPK) signaling axis. Our results suggest that therapeutic strategies targeting miR-106b-5p or downstream factors can be effective in preventing epileptogenesis or treating epilepsy.
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http://dx.doi.org/10.1007/s10753-022-01686-1DOI Listing
July 2022

Type-1 Grade 2 Multi-Focal Gastric Neuroendocrine Tumors Secondary to Chronic Autoimmune Gastritis.

Front Med (Lausanne) 2022 17;9:856125. Epub 2022 Jun 17.

Department of Gastroenterology and Hepatology, The First Affiliated Hospital of Nanchang Medical College, Jiangxi Provincial People's Hospital, Nanchang, China.

Background: Chronic autoimmune gastritis (CAG) refers to chronic atrophic gastritis due to autoimmunity. Loss of gastric glands in CAG results in hypergastrinemia and achlorhydria leading to Vitamin B deficiency and hyperplasia of G cells and enterochromaffin-like (ECL) cells. Vitamin B deficiency could cause pernicious anemia and subacute combined degeneration, while G cells and ECL cells hyperplasia might develop gastric neuroendocrine tumor (G-NET).

Case Presentation: A 35-year-old Chinese female presented with multi-focal type-1 Grade 2 (G2) NETs with a 14-year history of pernicious anemia and subacute combined degeneration.

Conclusion: Here, we report a rare case of a Chinese patient presenting G-NET combined with pernicious anemia and subacute combined degeneration, which are secondary to chronic autoimmune gastritis. This case also illustrates the importance of routine gastroscopy in patients with Vitamin B deficiency.
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http://dx.doi.org/10.3389/fmed.2022.856125DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9247202PMC
June 2022

Membrane-fusogenic biomimetic particles: a new bioengineering tool learned from nature.

J Mater Chem B 2022 Jul 4. Epub 2022 Jul 4.

Laboratory of Biomaterials and Translational Medicine, Center for Nanomedicine, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China.

Membrane fusion, a fundamental biological process of the fusion of the membrane composition between cells, is vital for cell-cell communication and cargo transport between living cells. This fusion interaction achieves the transportation of the inner content to the cellular cytosol as well as the simultaneous blending of foreign substances with the cell membrane. Inspired by this biological process, emerging membrane-fusogenic particles have been developed, opening a new area for bioengineering and biomedical applications. Especially, membrane-fusion-mediated transfer of inner cargoes can bypass endosomal entrapment to maximize the transportation efficiency, emerging as a unique cytoplasmic delivery platform distinct from those depending on conventional endocytosis-based pathways. In addition, the membrane fusion enables cell surface modification through lipid diffusion and mixing, providing a tool for direct cell membrane engineering. In this review, we focus on the development of membrane-fusogenic particles and their up-to-date progress. We briefly introduce the concept of membrane fusion, elaborate inspiring strategies of membrane-fusogenic particles, and highlight the recent advances and the promising applications of membrane-fusogenic particles as a next-generation bioengineering tool. In the end, we conclude with the present challenges and opportunities, providing insights in the future research of membrane-fusogenic particles.
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http://dx.doi.org/10.1039/d2tb00632dDOI Listing
July 2022

Pelvic bones ADC could help to predict severe hematologic toxicity in patients undergoing concurrent chemoradiotherapy for cervical cancer.

Magn Reson Imaging 2022 Jun 28. Epub 2022 Jun 28.

Department of Radiology, Cancer Hospital of China Medical University, Xiaoheyan road 44#, Shenyang Liaoning 110042, China. Electronic address:

Background: Hematologic toxicity (HT) during concurrent chemoradiotherapy (CCRT) for cervical cancer can lead to treatment breaks and compromise efficacy.

Purpose: To evaluate the association between severe hematologic toxicity (HT) and clinical factors and pelvic apparent diffusion coefficient (ADC) during CCRT of cervical cancer patients.

Methods: Data from 120 patients with cervical cancer who were treated with CCRT from January 2016 and December 2018 were retrospectively analyzed. The clinical data (age, menopausal status, clinical stage, body mass index, chemotherapy regimen and chemotherapy cycle) of the patients were collected, and the cohort were divided into two groups based on the HT grade: HT3+ group (HT grade ≥ 3; 66 patients) and HT3- group (HT grade<3; 54 patients). All patients performed MRI before CCRT, and pelvic (ilium, pubis, ischium) ADC value was measured on ADC map. The correlation between severe HT and clinical parameters and pelvic ADC value were analyzed by univariate analysis, and the diagnostic performance was further assessed by receiver operating characteristic (ROC) analysis.

Results: In univariate analysis, the menopausal status (p = 0.012) and chemotherapy regimen (p = 0.011) were significantly correlated with severe HT in overall patients, and menopausal patients or patients receiving paclitaxel plus cisplatin (TP) regimen were more likely to develop severe HT. HT3+ group showed a significantly lower pelvic ADC value than HT3- group. The ADC value cut-offs derived from our study for predicting severe HT was 0.317 × 10 mm/s in overall patients. Neither clinical parameters or pelvic ADCs were associated with severe HT in menopausal patients when analyzed separately (p > 0.05).

Conclusions: Severe HT was significantly associated with menopausal status and chemotherapy regimen in patients with cervical cancer treated with CCRT, and HT3+ group showed a lower pelvic ADC value.
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http://dx.doi.org/10.1016/j.mri.2022.06.007DOI Listing
June 2022

Superoxide dismutase-embedded metal-organic frameworks biomimetic mineralization for the treatment of inflammatory bowel disease.

J Mater Chem B 2022 07 13;10(27):5174-5181. Epub 2022 Jul 13.

Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, China.

Inflammatory bowel disease (IBD) is characterized by chronic and spontaneous inflammation in the gastrointestinal tract, and has been associated with an improved level of reactive oxygen species (ROS). Herein, superoxide dismutase (SOD) was encapsulated into zeolitic imidazolate framework-zni (ZIF-zni) to construct a nanocomposite termed [email protected] through biomimetic mineralization, which was then used as a formulation for the IBD treatment. The [email protected] composite could efficiently suppress the level of ROS and pro-inflammatory cytokines, using the colorectal cancer cell line SW480 as a model. Oral administration of the [email protected] composite could relieve the oxidative stress and inhibit the release of pro-inflammatory cytokines in the inflamed colonic tissues, leading to the alleviation of colitis in dextran sulfate sodium-induced colitis mice. Overall, the favorable therapeutic efficacy and biocompatibility of [email protected] gave it potential to be used as a safe and effective formulation for IBD treatment in the future.
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http://dx.doi.org/10.1039/d2tb00896cDOI Listing
July 2022

Analysis on the Policy Environment Influencing Factors of Chinese Environmental Education Development and the Reform Trend in Combination with Environmental Education History.

Authors:
Tao Yu Manyi Gu

J Environ Public Health 2022 21;2022:3454709. Epub 2022 Jun 21.

Southwest Medical University, School of Humanities and Management, Luzhou 646000, Sichuan, China.

At the turn of the century, facing the challenge of information and technology, environmental education, and talent competition, the world's major environmentally, educationally developed countries have entered the wave of environmental education reform. This was promulgated in 1999, and our country also successively promulgated through "the central committee of the communist party of China under the State Council on deepening environmenta education reform and comprehensively promote quality environmental education decision" in 2001 the "basic teaching environmental education course reform outline (try out)," launched to "deepen the environmental education reform and comprehensively promote quality environmental education" for the purpose of the foundation environmental education course and teaching reform. The curriculum reform is characterized by government leadership, expert guidance, and teacher participation. Its specific approach is top-down, overall promotion, concept guidance, experimental exploration, and gradual expansion, which has achieved a lot of theoretical and practical achievements. However, the practice of more than ten years has proved that the goal of the curriculum reform of basic environmental education has not been safely realized, the classroom has not undergone fundamental changes, and the advanced curriculum concept and curriculum system have not been transformed into advanced classroom teaching practice. This paper mainly discusses the influencing factors of the policy environment of environmental education development in China and analyzes the trend of reform in combination with the history of environmental education. On the one hand, the research of this paper can enrich the research theory of pedagogy; on the other hand, it can provide reference for the practical environmental education reform and have rich significance in theory and practice.
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http://dx.doi.org/10.1155/2022/3454709DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9239765PMC
July 2022

Prognostic value of high-frequency oscillations combined with multimodal imaging methods for epilepsy surgery.

Chin Med J (Engl) 2022 May 5;135(9):1087-1095. Epub 2022 May 5.

Beijing Institute of Functional Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing 100053, China.

Background: The combination of high-frequency oscillations (HFOs) with single-mode imaging methods has been proved useful in identifying epileptogenic zones, whereas few studies have examined HFOs combined with multimodal imaging methods. The aim of this study was to evaluate the prognostic value of ripples, an HFO subtype with a frequency of 80 to 200 Hz is combined with multimodal imaging methods in predicting epilepsy surgery outcome.

Methods: HFOs were analyzed in 21 consecutive medically refractory epilepsy patients who underwent epilepsy surgery. All patients underwent positron emission tomography (PET) and deep electrode implantation for stereo-electroencephalography (SEEG); 11 patients underwent magnetoencephalography (MEG). Sensitivity, specificity, positive predictive value, negative predictive value, and accuracy in predicting surgical outcome were calculated for ripples combined with PET, MEG, both PET and MEG, and PET combined with MEG. Kaplan-Meier survival analyses were conducted in each group to estimate prognostic value.

Results: The study included 13 men and 8 women. Accuracy for ripples, PET, and MEG alone in predicting surgical outcome was 42.9%, 42.9%, and 81.8%, respectively. Accuracy for ripples combined with PET and MEG was the highest. Resection of regions identified by ripples, MEG dipoles, and combined PET findings was significantly associated with better surgical outcome (P  < 0.05).

Conclusions: Intracranial electrodes are essential to detect regions which generate ripples and to remove these areas which indicate good surgical outcome for medically intractable epilepsy. With the assistance of presurgical noninvasive imaging examinations, PET and MEG, for example, the SEEG electrodes would identify epileptogenic regions more effectively.
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http://dx.doi.org/10.1097/CM9.0000000000001909DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9276102PMC
May 2022

Accelerated carbonate dissolution caused by anthropogenic acidification - contrast of watershed soils to lake sediments in Taihu Region, China.

Environ Sci Pollut Res Int 2022 Jun 30. Epub 2022 Jun 30.

College of Environmental Sciences and Engineering, Yangzhou University, Yangzhou, 225217, China.

Although Taihu watershed is an "acid-insensitive" region, anthropogenic acidification has greatly changed the water chemistry in Taihu Lake. However, how soil carbonates responded to the long-term human-induced acidification received less attention. In this work, we investigated soil carbonate concentrations from different land uses in the upstream of the lake and sediment carbonate profiles in the lake, to explore the linkage of carbonates dissolution in the land and sedimentation in the lake. The result showed that the wheat-rice surface soil, the most acidification-impacted by fertilization and acid deposition, had significantly lower pH than vegetable and wetland soils (p < 0.05). Meanwhile, the carbonate concentration in wetland soils, only impacted by acid deposition, was significantly higher than that in wheat-rice and vegetable soils (p < 0.05). The pH profile of fertilized soils, with an increasing trend from the surface to bottom, further indicated the acidifying effect of fertilization. Although the average soil pH across all land uses was 6.6 in the upstream of the lake, remaining carbonate buffering system, the significant carbonate decrease especially in surface soils evidenced the definite carbonate dissolution by acidification, which is cumulative and irreversible. Contrary to the topsoils, the sediment carbonate concentration presented an increasing trend from the depth of 15 cm (denoting around the early 1980s) to the surface, indicating that lake sediment is a major sink of carbonate Ca and Mg from the watershed, particular under an alkaline lake environment caused by frequent algae blooms in the past decades. In addition, Ca/Mg ratio in the sediment, having higher values in a higher pH environment, was quite different from the watershed soil pattern, suggesting different biogeochemical processes Ca and Mg underwent during their transportation and sedimentation. The effects of acidification-altered re-distribution of carbonate Ca and Mg and Ca/Mg ratio in the terrestrial and aquatic environments deserve wider considerations of ecosystem consequence.
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http://dx.doi.org/10.1007/s11356-022-21741-6DOI Listing
June 2022

Understanding Electron-Phonon Interactions in 3D Lead Halide Perovskites from the Stereochemical Expression of 6s Lone Pairs.

J Am Chem Soc 2022 Jul 29;144(27):12247-12260. Epub 2022 Jun 29.

Beijing National Laboratory for Molecular Science, State Key Laboratory of Rare Earth Materials Chemistry and Applications, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.

The electron-phonon (e-ph) interaction in lead halide perovskites (LHPs) plays a role in a variety of physical phenomena. Unveiling how the local lattice distortion responds to charge carriers is a critical step toward understanding the e-ph interaction in LHPs. Herein, we advance a fundamental understanding of the e-ph interaction in LHPs from the perspective of stereochemical activity of 6s lone-pair electrons on the Pb cation. We demonstrate a model system based on three LHPs with distinctive lone-pair activities for studying the structure-property relationships. By tuning the A-cation chemistry, we synthesized single-crystal CsPbBr, (MAEA)PbBr (MA = methylammonium; EA = ethylammonium), and (MHy)PbBr (MHy = methylhydrazinium), which exhibit stereo-inactive, dynamic stereo-active, and static stereo-active lone pairs, respectively. This gives rise to distinctive local lattice distortions and low-frequency vibrational modes. We find that the e-ph interaction leads to a blue shift of the band gap as temperature increases in the structure with the dynamic stereo-active lone pair but to a red shift in the structure with the static stereo-active lone pair. Furthermore, analyses of the temperature-dependent low-energy photoluminescence tails reveal that the strength of the e-ph interaction increases with increasing lone-pair activity, leading to a transition from a large polaron to a small polaron, which has significant influence on the emission spectra and charge carrier dynamics. Our results highlight the role of the lone-pair activity in controlling the band gap, phonon, and polaronic effect in LHPs and provide guidelines for optimizing the optoelectronic properties, especially for tin-based and germanium-based halide perovskites, where stereo-active lone pairs are more prominent than their lead counterparts.
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http://dx.doi.org/10.1021/jacs.2c03443DOI Listing
July 2022

A genome and gene catalog of glacier microbiomes.

Nat Biotechnol 2022 Jun 27. Epub 2022 Jun 27.

State Key Laboratory of Tibetan Plateau Earth System, Resources and Environment (TPESRE), Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing, China.

Glaciers represent a unique inventory of microbial genetic diversity and a record of evolution. The Tibetan Plateau contains the largest area of low-latitude glaciers and is particularly vulnerable to global warming. By sequencing 85 metagenomes and 883 cultured isolates from 21 Tibetan glaciers covering snow, ice and cryoconite habitats, we present a specialized glacier microbial genome and gene catalog to archive glacial genomic and functional diversity. This comprehensive Tibetan Glacier Genome and Gene (TG2G) catalog includes 883 genomes and 2,358 metagenome-assembled genomes, which represent 968 candidate species spanning 30 phyla. The catalog also contains over 25 million non-redundant protein-encoding genes, the utility of which is demonstrated by the exploration of secondary metabolite biosynthetic potentials, virulence factor identification and global glacier metagenome comparison. The TG2G catalog is a valuable resource that enables enhanced understanding of the structure and functions of Tibetan glacial microbiomes.
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http://dx.doi.org/10.1038/s41587-022-01367-2DOI Listing
June 2022

MRI-based radiomics nomogram for the preoperative prediction of deep myometrial invasion of FIGO I stage endometrial carcinoma.

Med Phys 2022 Jun 27. Epub 2022 Jun 27.

Radiology Department, Liaoning Cancer Hospital & Institute, China Medical University, Shenyang, Liaoning, 110042, China.

Background: Endometrial carcinoma (EC) is one of the most common gynecological malignancies with an increasing incidence, and an accurate preoperative diagnosis of deep myometrial invasion (DMI) is crucial for personalized treatment.

Objective: To determine the predictive value of an MRI-based radiomics nomogram for the presence of DMI in FIGO I stage EC.

Methods: We retrospectively collected 163 patients with pathologically confirmed stage I EC from two centers and divided all samples into a training group (center 1) and a validation group (center 2). Clinical and routine imaging indicators were analyzed by logistical regression to construct a conventional diagnostic model (M1). Radiomics features extracted from the axial T2-weighted (T2W) and axial contrast-enhanced T1-weighted (CE-T1W) images were treated with the intraclass correlation coefficient, Mann-Whitney U test, least absolute shrinkage and selection operator (LASSO), and logistic regression analysis with Akaike information criterion (AIC) to build a combined radiomics signature (M2). A nomogram (M3) was constructed by M1 and M2. Calibration and decision curves were drawn to evaluate the nomogram in the training and validation cohorts. The diagnostic performance of each indicator and model was evaluated by the area under the receiver operating characteristic curve (AUC).

Result: The four most significant radiomics features were finally selected from the CE-T1W MRI. For the diagnosis of DMI, the AUC /AUC of M1 was 0.798/0.738, the AUC /AUC of M2 was 0.880/0.852, and the AUC /AUC of M3 was 0.936/0.871 in the training and validation groups, respectively. The calibration curves showed that M3 was in good agreement with the ideal values. The decision curve analysis suggested potential clinical application values of the nomogram.

Conclusion: A nomogram based on MRI radiomics and clinical imaging indicators can improve the diagnosis of DMI in patients with FIGO I stage EC. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/mp.15835DOI Listing
June 2022

A model for predicting high BMI of people living with HIV after receiving antiretroviral therapy.

Ther Adv Chronic Dis 2022 21;13:20406223221102750. Epub 2022 Jun 21.

Department of Infectious Diseases, Nanfang Hospital, Southern Medical University, No. 1838, Guangzhou Avenue North, Guangzhou 510515, Guangdong, China.

Objective: The objective of this study was to evaluate the characteristics of high body mass index (BMI) and normal weight people living with HIV after antiretroviral therapy (ART) and establish a model.

Methods: A total of 290 people living with HIV after 1 year of ART treatment were enrolled and divided into two groups based on whether their BMI index was <24 or ⩾24 at week 48. The demographic, clinical data were collected and analyzed. Multivariable logistic regression analysis was performed. A model was established and use to predict the occurrence of certain diseases.

Results: A total of 290 people living with HIV were included in this study; 200 had a normal BMI (BMI < 24) and 90 were high BMI (BMI ⩾ 24) after 1-year ART. Their baseline characteristics were significantly different in relation to age ( = 0.007), sex distribution ( = 0.040), ART regimen ( = 0.040), alanine aminotransferase levels ( < 0.001), and three major serum lipid levels: triglycerides ( < 0.001), cholesterol ( = 0.011), and low-density lipoprotein ( = 0.005). A multivariate logistic regression analysis resulted in the development of a model for the diagnosis of high BMI and hyperlipidemia. The model score is an independent risk factor for hyperlipidemia (odds ratio = 2.674,  = 0.001) and high BMI ( < 0.001). The model score is significantly correlated with the controlled attenuation parameter (CAP) value ( = 0.230,  < 0.001) and can be used to divide the severity of liver steatosis based on CAP value.

Conclusions: This study demonstrated a easy-to-use model to detect high BMI, hyperlipidemia, and liver steatosis in people living with HIV without risk factors for BMI changing at baseline after 1 year of ART treatment.
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http://dx.doi.org/10.1177/20406223221102750DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218435PMC
June 2022
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