Publications by authors named "Tao Ye"

868 Publications

Association between age and incidence of deep vein thrombosis in patients with spinal cord injury: an observational cross-sectional study.

Spinal Cord 2022 May 24. Epub 2022 May 24.

Rehabilitation Department, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui Province, 230031, PR China.

Study Design: Cross-sectional study.

Objective: To elucidate the association between age and incidence of deep vein thrombosis (DVT) in patients with spinal cord injury (SCI).

Setting: Rehabilitation Medicine Department of the First Affiliated Hospital of China University of Science and Technology.

Methods: Patients from August 2018 to December 2020 with SCI (N = 260) were tertiles divided the age into three groups to analyze the association between age and incidence of DVT.

Results: American Spinal Cord Injury Association impairment scale (AIS), urinary tract infection (UTI), pulmonary infection (PI), and anticoagulation therapy (AT) were confounders for the association between age and incidence of DVT. The incidence of DVT increased by 1.07-fold (Non-adjusted model, OR = 1.07, P < 0.001), 1.05-fold (Minimally-adjusted model: adjusted for confounders, OR = 1.05, P = 0.010) and 1.06-fold [Fully-adjusted model: adjusted for confounders and unbalanced probable variables: AIS, UTI, PI, AT, Sex, D-dimer(new), Fibrinogen (new), Modes of injury and Level of injury, OR = 1.06, P = 0.012] when age increased by 1 year. The incidence of DVT had an increasing trend with age in different age tertile in the three models (P for trend <0.05). Age had a linearly association with incidence of DVT (OR = 1.07, P = 0.065) and stable in different subgroups, for lower age, the association was also linearly (OR = 4.40, P = 1.000), for middle (fold point = 46.46, P < 0.001) and higher age (fold point = 66, P = 0.017), the association was curvilinear.

Conclusion: Age had a linearly association with incidence of DVT. Quitting smoking, preventing/treating UTI and AT should be adopted in advance for patients with SCI for all age, especially for older.
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http://dx.doi.org/10.1038/s41393-022-00814-wDOI Listing
May 2022

Achieving Balanced Electrical Performance of Host Material through Dual N-P═O Resonance Linkage for Efficient Electroluminescence.

ACS Appl Mater Interfaces 2022 May 24. Epub 2022 May 24.

State Key Laboratory of Organic Electronics and Information Displays, Institute of Advanced Materials (IAM), Nanjing University of Posts & Telecommunications, 9 Wenyuan Road, Nanjing 210023, China.

Developing high-performance host materials is one of the biggest challenges for blue and white thermally activated delayed-fluorescence (TADF) organic light-emitting diode (OLED) technology due to the rigorous requirements of both efficient carrier flux ability and high triplet energy () levels in static donor-acceptor molecules. Here, with the aid of a dual-resonance strategy, a host molecule showing dynamic adaption features in the acceptor-resonance-donor-resonance-acceptor (A-r-D-r-A) molecular configuration has been successfully developed through the implantation of two acceptors of diphenylphosphine oxide into electron-donating 5,10-dihydrophenazine with N-P═O resonance linkages. Owing to the dual enantiotropic N═P-O resonances, the designed A-r-D-r-A molecule exhibits an extraordinarily balanced charge flux transportation attribute at high (2.96 eV). Excitingly, blue and warm-white TADF OLEDs hosted by the A-r-D-r-A molecule exhibit outstanding external quantum efficiencies of 14.7 and 20.3%, respectively. Our studies not only broaden the scope of resonance molecules but also indicate that a resonance structure is an effective linkage to develop optoelectronic materials with dynamically adaptive properties.
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http://dx.doi.org/10.1021/acsami.2c02745DOI Listing
May 2022

The MYB transcription factor MYB103 acts upstream of TRICHOME BIREFRINGENCE-LIKE27 in regulating aluminium sensitivity by modulating the O-acetylation level of cell wall xyloglucan in Arabidopsis thaliana.

Plant J 2022 May 21. Epub 2022 May 21.

State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China.

Modification of the O-acetylation level of xyloglucan (XyG) appears to affect aluminium (Al) sensitivity in Arabidopsis by modulating its binding capacity to Al. However, the transcriptional regulation of this process remains largely unknown. In our previous studies, we found that the expression of TRICHOME BIREFRINGENCE-LIKE27 (TBL27), which is responsible for the O-acetylation of XyG, was downregulated under Al stress. In the present study, we showed that the expression of an R2R3-type transcription factor, MYB103, was also inhibited by Al exposure and exhibited a co-expression pattern with TBL27 in roots and siliques, suggesting a potential crosslink between MYB103 and TBL27. The loss of function of MYB103 resulted in increased Al sensitivity, as indicated by more inhibited root growth and elevated root Al content compared with that of the wild-type (WT). Moreover, we also detected increased Al accumulation in the root cell wall and hemicellulose fraction, which was attributed to the changes in the O-acetylation level of XyG rather than the XyG content itself. In addition, further analysis revealed that MYB103 positively activated TBL27 expression by directly binding to the TBL27 promoter region, and TBL27 overexpression in the myb103 mutant rescued the Al-sensitive phenotype of the mutant to the WT level. Taken together, we conclude that MYB103 acts upstream of TBL27 to positively regulate Al resistance by modulating the O-acetylation of the cell wall XyG.
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http://dx.doi.org/10.1111/tpj.15837DOI Listing
May 2022

Recombinant expression a novel fibronectin-collage fusion peptide modulating stem cell stemness via integrin β3.

Appl Microbiol Biotechnol 2022 May 20. Epub 2022 May 20.

Institute of Biomedicine and Guangdong Provincial Key Laboratory of Bioengineering Medicine, Jinan University, Guangzhou, 510632, China.

Constructing bionic extracellular matrix (ECM) is an attractive proposition for tissue engineering and clinical regeneration therapy involving the stemness of stem cells. Here, a novel recombinant protein fibronectin-collagen peptide (FCP) was designed to modulate the function of ECM expressed by Picha. pastoris strain X33. This FCP promotes cell migration and adhesion and maintains rBMSC stemness by binding integrin β3. Its effects were blocked by both integrin β3 siRNA and the integrin β3 inhibitor Cilengitide. A template-independent ab initio prediction modeling approach is the best approach to construct a stable FCP protein model, which predicts the binding sites between FCP and integrin β3. FCP may be used in the in vitro culture and clinical regeneration of stem cells that highly express integrin β3, such as hematopoietic stem cells. The study provides information on the molecular structure of FCP and its bioactivity, which can be used to design new compounds. KEY POINTS: • Design a novel recombinant fibronectin-collagen peptide biomimetic ECM. • FCP promotes cell adhesion, migration, and proliferation. • Predicted and verified FCP structure and affinity with integrin β3. • FCP binds integrin β3 to maintain rBMSC stemness.
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http://dx.doi.org/10.1007/s00253-022-11965-4DOI Listing
May 2022

Hydrogen chloride (HCl) at ground sites during CalNex 2010 and insight into its thermodynamic properties.

J Geophys Res Atmos 2022 Apr;127(9):1-16

Chemical Sciences Laboratory, Earth System Research Laboratory, NOAA, Boulder, Colorado, USA.

Gas phase hydrogen chloride (HCl) was measured at Pasadena and San Joaquin Valley (SJV) ground sites in California during May and June 2010 as part of the CalNex study. Observed mixing ratios were on average 0.83 ppbv at Pasadena, ranging from below detection limit (0.055 ppbv) to 5.95 ppbv, and were on average 0.084 ppbv at SJV with a maximum value of 0.776 ppbv. At both sites, HCl levels were highest during midday and shared similar diurnal variations with HNO. Coupled phase partitioning behavior was found between HCl/Cl and HNO/NO using thermodynamic modelling and observations. Regional modeling of Cl and HCl using CMAQ captures some of the observed relationships but underestimates measurements by a factor of 5 or more. Chloride in the 2.5-10 μm size range in Pasadena was sometimes higher than sea salt abundances, based on co-measured Na, implying that sources other than sea salt are important. The acid-displacement of HCl/Cl by HNO/NO (phase partitioning of semi-volatile acids) observed at the SJV site can only be explained by aqueous phase reaction despite low RH conditions and suggests the temperature dependence of HCl phase partitioning behavior was strongly impacted by the activity coefficient changes under relevant aerosol conditions (e.g., high ionic strength). Despite the influence from activity coefficients, the gas-particle system was found to be well constrained by other stronger buffers and charge balance so that HCl and Cl concentrations were reproduced well by thermodynamic models.
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http://dx.doi.org/10.1029/2021jd036062DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9109133PMC
April 2022

Ginsenoside Rb1 Improves Metabolic Disorder in High-Fat Diet-Induced Obese Mice Associated With Modulation of Gut Microbiota.

Front Microbiol 2022 19;13:826487. Epub 2022 Apr 19.

State Key Laboratory of Genetic Engineering, Department of Microbiology and Immunology, School of Life Sciences, Fudan University, Shanghai, China.

Gut microbiota plays an important role in metabolic homeostasis. Previous studies demonstrated that ginsenoside Rb1 might improve obesity-induced metabolic disorders through regulating glucose and lipid metabolism in the liver and adipose tissues. Due to low bioavailability and enrichment in the intestinal tract of Rb1, we hypothesized that modulation of the gut microbiota might account for its pharmacological effects as well. Here, we show that oral administration of Rb1 significantly decreased serum LDL-c, TG, insulin, and insulin resistance index (HOMA-IR) in mice with a high-fat diet (HFD). Dynamic profiling of the gut microbiota showed that this metabolic improvement was accompanied by restoring of relative abundance of some key bacterial genera. In addition, the free fatty acids profiles in feces were significantly different between the HFD-fed mice with or without Rb1. The content of eight long-chain fatty acids (LCFAs) was significantly increased in mice with Rb1, which was positively correlated with the increase of and , and negatively correlated with the decrease of and . Among these eight increased LCFAs, eicosapentaenoic acid (EPA), octadecenoic acids, and myristic acid were positively correlated with metabolic improvement. Furthermore, the colonic expression of the () gene was significantly upregulated after Rb1 treatment, in response to a notable increase of LCFA in feces. These findings suggested that Rb1 likely modulated the gut microbiota and intestinal free fatty acids profiles, which should be beneficial for the improvement of metabolic disorders in HFD-fed mice. This study provides a novel mechanism of Rb1 for the treatment of metabolic disorders induced by obesity, which may provide a therapeutic avenue for the development of new nutraceutical-based remedies for treating metabolic diseases, such as hyperlipidemia, insulin resistance, and type 2 diabetes.
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http://dx.doi.org/10.3389/fmicb.2022.826487DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9062662PMC
April 2022

The NAC transcription factor ANAC017 regulates aluminum tolerance by regulating the cell wall-modifying genes.

Plant Physiol 2022 May 4. Epub 2022 May 4.

State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Science, Nanjing, 210008, China.

Aluminum (Al) toxicity is one of the key factors limiting crop production in acid soils; however, little is known about its transcriptional regulation in plants. In this study, we characterized the role of a NAM, ATAF1/2, and cup-shaped cotyledon 2 (NAC) transcription factors, ANAC017, in the regulation of Al tolerance in Arabidopsis (Arabidopsis thaliana). ANAC017 was localized in the nucleus and exhibited constitutive expression in the root, stem, leaf, flower and silique, although its expression and protein accumulation were repressed by Al stress. Loss of function of ANAC017 enhanced Al tolerance when compared with wild-type Col-0 and was accompanied by lower root and root cell wall Al content. Furthermore, both hemicellulose and xyloglucan content decreased in the anac017 mutants, indicating the possible interaction between ANAC017 and xyloglucanendotransglucosylase/hydrolase (XTH). Interestingly, the expression of XTH31, which is responsible for xyloglucan modification, was down-regulated in the anac017 mutants regardless of Al supply, supporting the possible interaction between ANAC017 and XTH31. Yeast one-hybrid, dual-luciferase reporter assay, and ChIP-qPCR analysis revealed that ANAC017 positively regulated the expression of XTH31 through directly binding to the XTH31 promoter region, and overexpression of XTH31 in the anac017 mutant background rescued its Al-tolerance phenotype. In conclusion, we identified that the transcription factor ANAC017 acts upstream of XTH31 to regulate Al tolerance in Arabidopsis.
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http://dx.doi.org/10.1093/plphys/kiac197DOI Listing
May 2022

Mamba: a systematic software solution for beamline experiments at HEPS.

J Synchrotron Radiat 2022 May 5;29(Pt 3):664-669. Epub 2022 Apr 5.

Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, People's Republic of China.

To cater for the diverse experiment requirements at the High Energy Photon Source (HEPS) with often limited human resources, Bluesky was chosen as the basis for our software framework, Mamba. In our attempt to address Bluesky's lack of integrated graphical user interfaces (GUIs), command injection with feedback was chosen as the main way for the GUIs to cooperate with the command line interface; a remote-procedure-call service is also provided, which covers functionalities unsuitable for command injection, as well as pushing of status updates. In order to fully support high-frequency applications like fly scans, Bluesky's support for asynchronous control is being improved; furthermore, to support high-throughput experiments, Mamba Data Worker is being developed to cover the complexity in asynchronous online data processing for these experiments. To systematically simplify the specification of metadata, scan parameters and data-processing graphs for each type of experiment, an experiment parameter generator will be developed; experiment-specific modules to automate preparation steps will also be made. The integration of off-the-shelf code in Mamba for domain-specific needs is under investigation, and Mamba GUI Studio is being developed to simplify the implementation and integration of GUIs.
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http://dx.doi.org/10.1107/S1600577522002697DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9070726PMC
May 2022

A tripodal wheeled mobile robot driven by a liquid metal motor.

Lab Chip 2022 May 17;22(10):1943-1950. Epub 2022 May 17.

State Key Laboratory of Robotics and System, Harbin Institute of Technology, West Da-zhi Street 92, Harbin, Heilongjiang 150001, People's Republic of China.

As a novel driving concept, the liquid metal motor (LMM) has been regarded as a promising actuator due to its unique traits, such as infinitely variable speed, lack of transmission chain, convenient maintenance, and silence. However, at present, driving devices based on this material are still in the preliminary and rudimentary stage, and representative application examples are scarce. Therefore, an 8-shaped tripodal wheeled mobile robot (WMR) completely driven by a LMM is designed in this study to further prove the practicability of this material. Through combining the Marangoni surface flow on a liquid metal droplet (LMD) caused by an electrochemical reaction and the eccentric torque generated by the change in droplet shape and position, the two independently driven wheels of the mobile robot are actuated at differential moving speeds. Additionally, a matching control module, a cell phone application, and a battery have been developed and added for wireless control of three types of driving functions (moving forward, steering, and stopping). It is expected that this work could further advance the development and application of LMMs and bring new ideas to the design of WMRs.
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http://dx.doi.org/10.1039/d2lc00267aDOI Listing
May 2022

Effects of Cinacalcet and Parathyroidectomy on Blood Pressure in Maintenance Hemodialysis Patients with Secondary Hyperparathyroidism.

Iran J Kidney Dis 2022 03;16(2):135-146

Nephrology Department, Huashan Hospital, Fudan University, Shanghai, China.

Introduction: Secondary hyperparathyroidism may cause an increase in blood pressure among maintenance hemodialysis (MHD) patients. The objective of this study were to observe the effects of different treatment modalities of hyperparathyroidism on blood pressure among MHD patients with secondary hyperparathyroidism.

Methods: This retrospective cohort study was conducted on 69 patients divided into three groups, based on the therapeutic strategies (parathyroidectomy, n = 22; cinacalcet, n = 14; calcitriol, n = 33). Changes in systolic blood pressure (SBP) and diastolic blood pressure (DBP) from pre- to post-treatment visits at 1st, 3rd, 6th, and 12th month were analyzed by mixed-effects repeated-measures model. Serum levels of the renin-angiotensin system (RAS) mediators (renin and aldosterone), endothelin, and echocardiography were compared before and after one year of treatment within the three groups.

Results: Changes in blood pressure were significantly different among the three groups (SBP: P for group < 0.05; DBP: P for group < .05; both P for group × time interaction < .05). SBP and DBP showed a significant downward trend in the surgery group (P for change in SBP < .05, P for change in DBP < .001, adjusted mean change of SBP = -12.16 (-19.70 to -4.62) mmHg and of DBP = -6.82 (-10.58 to -3.06) mmHg in the surgery group on the 12th month). Diastolic BP showed a significant upward trend in the cinacalcet group (P for change in DBP < .05, adjusted mean change of DBP = 6.03 (2.08 to 9.98) mmHg in cinacalcet group in the 12th month). No significant change in BP was observed in the calcitriol group. The levels of serum RAS mediators, endothelin, or cardiac ultrasonography didn't change and almost remained consistent during the treatment course.

Conclusion: Blood pressure decreased significantly over a year in patients with parathyroidectomy, while DBP increased significantly over time by cinacalcet treatment.  DOI: 10.52547/ijkd.6686.
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March 2022

New SFT2-like Vesicle Transport Protein (SFT2L) Enhances Cadmium Tolerance and Reduces Cadmium Accumulation in Common Wheat Grains.

J Agric Food Chem 2022 May 28;70(18):5526-5540. Epub 2022 Apr 28.

College of Life Science and Technology/Henan Engineering Research Center of Crop Genome Editing/Henan International Joint Laboratory of Plant Genetic Improvement and Soil Remediation/Collaborative Innovation Center of Modern Biological Breeding of Henan Province, Henan Institute of Science and Technology, Xinxiang 453003, China.

Cadmium (Cd) is one of the most toxic heavy metal elements to the environment, which seriously threatens the safe production of food crops. In this study, we identified a novel function of the cytomembrane TaSFT2L protein in wheat (). Expression of the gene in yeast showed no transport activities for Cd, which could explain the role of in metal tolerance. It was observed that increased autophagic activity in roots caused by silencing of enhanced Cd tolerance. Transgenic wheat revealed that RNA interference (RNAi) lines enhanced the wheat growth concerning the increased shoot or root elongation, dry weight, and chlorophyll accumulation. Furthermore, RNAi lines decreased root-to-grain Cd translocation in wheat by nearly 68% and Cd accumulation in wheat grains by 53%. Meanwhile, the overexpression lines displayed a compromised growth response and increased Cd accumulation in wheat tissues, compared to wild type. These findings show that is a key gene involved in regulation of Cd translocation in wheat, and its silencing to form transgenic wheat can inhibit Cd accumulation. This has the ability to alleviate the food chain-associated impact of environmental pollution on human health.
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http://dx.doi.org/10.1021/acs.jafc.1c08021DOI Listing
May 2022

Resolution-enhanced ptychography framework with an equivalent upsampling and precise position.

Appl Opt 2022 Apr;61(10):2903-2909

As a lensless imaging technique, ptychography provides a new way to resolve the conflict between the spatial resolution and the field of view. However, due to the pixel size limit of the sensor, a compromise has to be reached between the spatial resolution and the signal-to-noise ratio. Here, we propose a resolution-enhanced ptychography framework with equivalent upsampling and subpixel accuracy in position to further improve the resolution of ptychography. According to the theory of pixel superresolved techniques, the inherent shift illumination scheme in ptychography can additionally enhance the resolution with the redundant data. An additional layer of pooling is used to simulate the downsampling of a digital record, and the pixel superresolved problem is transformed into an automatic optimization problem. The proposed framework is verified by optical experiments, both in biological samples and the resolution targets. Compared to the traditional algorithm, the spatial lateral resolution is twice as large using the same data set.
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http://dx.doi.org/10.1364/AO.451431DOI Listing
April 2022

Mechanisms of Cell Adhesion Molecules in Endocrine-Related Cancers: A Concise Outlook.

Front Endocrinol (Lausanne) 2022 7;13:865436. Epub 2022 Apr 7.

Department of Pediatrics, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Chemotherapy is a critical treatment for endocrine-related cancers; however, chemoresistance and disease recurrence remain a challenge. The interplay between cancer cells and the tumor microenvironment cell adhesion molecules (CAMs) promotes drug resistance, known as cell adhesion-mediated drug resistance (CAM-DR). CAMs are cell surface molecules that facilitate cell-to-cell or cell-to-extracellular matrix binding. CAMs exert an adhesion effect and trigger intracellular signaling that regulates cancer cell stemness maintenance, survival, proliferation, metastasis, epithelial-mesenchymal transition, and drug resistance. To understand these mechanisms, this review focuses on the role of CD44, cadherins, selectins, and integrins in CAM-DR in endocrine-related cancers.
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http://dx.doi.org/10.3389/fendo.2022.865436DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9021432PMC
April 2022

Discovery of Phospholipase D Inhibitors with Improved Drug-like Properties and Central Nervous System Penetrance.

ACS Med Chem Lett 2022 Apr 3;13(4):665-673. Epub 2022 Mar 3.

Biotherapeutic and Medicinal Sciences, Neuromuscular & Movement Disorders Research Unit, Research and Early Development Biomarkers, Biogen, 225 Binney Street, Cambridge, Massachusetts 02142, United States.

Phospholipase D (PLD) is a phospholipase enzyme responsible for hydrolyzing phosphatidylcholine into the lipid signaling molecule, phosphatidic acid, and choline. From a therapeutic perspective, PLD has been implicated in human cancer progression as well as a target for neurodegenerative diseases, including Alzheimer's. Moreover, knockdown of PLD rescues the ALS phenotype in multiple models of ALS (amyotrophic lateral sclerosis) and displays modest motor benefits in an SOD1 ALS mouse model. To further validate whether inhibiting PLD is beneficial for the treatment of ALS, a brain penetrant small molecule inhibitor with suitable PK properties to test in an ALS animal model is needed. Using a combination of ligand-based drug discovery and structure-based design, a dual PLD1/PLD2 inhibitor was discovered that is single digit nanomolar in the Calu-1 cell assay and has suitable PK properties for studies. To capture the measurement of PLD inhibition, a transphosphatidylation pharmacodynamic LC-MS assay was developed, in which a dual PLD1/PLD2 inhibitor was found to reduce PLD activity by 15-20-fold.
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http://dx.doi.org/10.1021/acsmedchemlett.1c00682DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9014516PMC
April 2022

A new handheld fundus camera combined with visual artificial intelligence facilitates diabetic retinopathy screening.

Int J Ophthalmol 2022 18;15(4):620-627. Epub 2022 Apr 18.

Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200080, China.

Aim: To explore the performance in diabetic retinopathy (DR) screening of artificial intelligence (AI) system by evaluating the image quality of a handheld Optomed Aurora fundus camera in comparison to traditional tabletop fundus cameras and the diagnostic accuracy of DR of the two modalities.

Methods: Overall, 630 eyes were included from three centers and screened by a handheld camera (Aurora, Optomed, Oulu, Finland) and a table-top camera. Image quality was graded by three masked and experienced ophthalmologists. The diagnostic accuracy of the handheld camera and AI system was evaluated in assessing DR lesions and referable DR.

Results: Under nonmydriasis status, the handheld fundus camera had better image quality in centration, clarity, and visible range (1.47, 1.48, and 1.40) than conventional tabletop cameras (1.30, 1.28, and 1.18; <0.001). Detection of retinal hemorrhage, hard exudation, and macular edema were comparable between the two modalities, in principle, with the area under the curve of the handheld fundus camera slightly lower. The sensitivity and specificity for the detection of referable DR with the handheld camera were 82.1% (95%CI: 72.1%-92.2%) and 97.4% (95%CI: 95.4%-99.5%), respectively. The performance of AI detection of DR using the Phoebus Algorithm was satisfactory; however, Phoebus showed a high sensitivity (88.2%, 95%CI: 79.4%-97.1%) and low specificity (40.7%, 95%CI: 34.1%-47.2%) when detecting referable DR.

Conclusion: The handheld Aurora fundus camera combined with autonomous AI system is well-suited in DR screening without mydriasis because of its high sensitivity of DR detection as well as its image quality, but its specificity needs to be improved with better modeling of the data. Use of this new system is safe and effective in the detection of referable DR in real world practice.
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http://dx.doi.org/10.18240/ijo.2022.04.16DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8995718PMC
April 2022

The effects of the oral administration of graphene oxide on the gut microbiota and ultrastructure of the colon of mice.

Ann Transl Med 2022 Mar;10(6):278

Department of General Surgery, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai, China.

Background: Graphene oxide (GO) has been widely used in the field of biomedicine and has shown great potential in drug delivery. Oral administration is an important mode of administration, but there are few studies on the effects of oral GO on gastrointestinal tract and gut microbiota. This study sought to explore the effects of oral GO on the gastrointestinal tract and gut microbiota.

Methods: In total, 20 C57BL/6 male mice, aged 5 weeks old, were randomly divided into the following 4 groups (n=5): the control group, the GO30 group, the GO60 group, and the GO120 group. The GO sample solution was administered intragastrically at the doses of 30, 60, or 120 mg/kg every 3 days, and the control group was given an equal volume of distilled water. On the 16th day, mouse feces were taken for 16S ribosomal ribonucleic acid (rRNA) sequencing analysis, and the mice were dissected, and the heart, liver, kidney, and colon removed for histological analysis. Additionally, the ultrastructure of the colon was observed by transmission electron microscopy.

Results: No obvious damage was observed in the hearts, livers, and kidneys of the mice. However, the intestinal ultrastructure of the mice in the GO group was damaged. The main manifestations were an uneven arrangement and local atrophy of the microvilli, swelling of the mitochondria and endoplasmic reticulum, and the widening of the intercellular spaces. The damage was positively correlated with increasing GO doses. The 16S rRNA sequencing results showed that the structure of the gut microbiota in the GO group was altered, and the contents of and were decreased.

Conclusions: The oral administration of GO had no obvious toxicity effects on the hearts, livers, and kidneys of the mice. However, it did destroy the ultrastructure of the mouse colon and shift the structure of the gut microbiota, decreasing the contents of , and .
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http://dx.doi.org/10.21037/atm-22-922DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9011234PMC
March 2022

Thyroid hormones disturbances, cognitive deficits and abnormal dynamic functional connectivity variability of the amygdala in unmedicated bipolar disorder.

J Psychiatr Res 2022 06 24;150:282-291. Epub 2022 Mar 24.

Medical Imaging Center, First Affiliated Hospital of Jinan University, Guangzhou, 510630, China; Institute of Molecular and Functional Imaging, Jinan University, Guangzhou, 510630, China. Electronic address:

Objective: Accumulating evidence suggests that hypothalamus-pituitary-thyroid (HPT) axis dysfunction is relevant to the neuropsychological and pathophysiology functions of bipolar disorder (BD). However, no research has investigated the inter-relationships among thyroid hormones disturbance, neurocognitive deficits, and aberrant brain function (particularly in the amygdala) in patients with BD.

Materials And Methods: Data of dynamic resting-state functional connectivity (rs-dFC) were gathered from 59 patients with unmedicated BD II during depressive episodes and 52 healthy controls (HCs). Four seeds were selected (the bilateral lateral amygdala and the bilateral medial amygdala). The sliding-window analysis was applied to investigate dynamic functional connectivity (dFC). Additionally, the serum thyroid hormone (free tri-iodothyronine (FT3), total tri-iodothyronine (TT3), free thyroxin (FT4), total thyroxin (TT4) and thyroid-stimulating hormone (TSH)) levels, and cognitive scores on the MATRICS Consensus Cognitive Battery (MCCB) in patients and HCs were detected.

Results: The BD group exhibited increased dFC variability between the left medial amygdala and right medial prefrontal cortex (mPFC) when compared with the HC group. Additionally, the BD group showed lower FT3, TT3, and TSH level, higher FT4 level, and poorer cognitive score. Moreover, a significant negative correlation was observed between the dFC variability of the left medial amygdala-right mPFC and TSH level, or reasoning and problem solving of MCCB score in BD group. Multiple regression analysis showed that the TSH level × dFC variability of the medial amygdala-mPFC was an independent predictor for cognitive processing speed in BD group.

Conclusions: This study revealed patients with BD II depression had excessive variability in dFC between the medial amygdala and mPFC. Moreover, both HPT axis dysfunction and abnormal dFC of the amygdala-mPFC might be implicated in cognitive impairment in the early stages of BD.
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http://dx.doi.org/10.1016/j.jpsychires.2022.03.023DOI Listing
June 2022

On-demand modulating afterglow color of water-soluble polymers through phosphorescence FRET for multicolor security printing.

Sci Adv 2022 04 15;8(15):eabk2925. Epub 2022 Apr 15.

State Key Laboratory of Organic Electronics and Information Displays and Institute of Advanced Materials (IAM), Jiangsu National Synergistic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications, 9 Wenyuan Road, Nanjing 210023, China.

Developing full-color organic ultralong room temperature phosphorescence (OURTP) materials with continuously variable afterglow emission is of considerable practical importance in diverse optoelectronic applications but remains a formidable challenge. Here, we present an effective strategy for on-demand engineering of afterglow color in water-soluble polymeric systems via efficient phosphorescence Förster resonance energy transfer. Using a blue afterglow emitting water-soluble polymer as host and a series of fluorescent emitters with varied emissive colors as guests, afterglow emission is rationally modulated, conferring the full-color afterglow emission ranging from blue to red and even white with ultralong lifetimes up to 4.2 s and photoluminescence quantum yields of 36%.These water-soluble multicolor-emitting polymeric afterglow systems can function as OURTP security inks, and multilevel information encryption was successfully established by RGB-based multicolor security printing. These results present important guidance in developing high-performance afterglow polymers with on-demand color tuning ability for remarkable optoelectronic applications.
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http://dx.doi.org/10.1126/sciadv.abk2925DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9012460PMC
April 2022

Design of ternary additive for organic photovoltaics: a cautionary tale.

RSC Adv 2022 Mar 30;12(16):10029-10036. Epub 2022 Mar 30.

Department of Chemical & Biological Engineering, University of Ottawa 161 Louis Pasteur Ottawa ON K1N 6N5 Canada

Silicon phthalocyanines as ternary additives are a promising way to increase the performance of organic photovoltaics. The miscibility of the additive and the donor polymer plays a significant role in the enhancement of the device performance, therefore, ternary additives can be designed to better interact with the conjugated polymer. We synthesized -9'-heptadecanyl-2,7-carbazole functionalized SiPc ((CBzPho)-SiPc), a ternary additive with increased miscibility in poly[-90-heptadecanyl-2,7-carbazole-alt-5,5-(4',7'-di-2-thienyl-2',1',3'-benzothiadiazole)] (PCDTBT). The resulting additive was included into PCDTBT and [6,6]-phenyl C butyric acid methyl ester as bulk (PCBM) heterojunction OPV devices as a ternary additive. While the (CBzPho)-SiPc demonstrated strong EQE >30% contribution in the range of 650-730 nm, the overall performance was reduced because (CBzPho)-SiPc acted as a hole trap due to its high-lying HOMO energy level. This study demonstrates the importance of the solubility, miscibility, and energy level engineering of the ternary additive when designing organic photovoltaic devices.
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http://dx.doi.org/10.1039/d2ra00540aDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8965687PMC
March 2022

Saponin composition comparison of black ginseng and white ginseng by liquid chromatography-mass spectrometry combined with multivariate statistical analysis.

Nat Prod Res 2022 Apr 14:1-5. Epub 2022 Apr 14.

Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, China.

Black ginseng (BG) is one type of ginseng product, which is produced from fresh ginseng by steaming and drying several times. To characterize the differences in saponin composition of BG and white ginseng (WG), the ultra-high performance liquid chromatography Quadrupole-Orbitrap mass spectrometry (UHPLC-Q-Orbitrap-MS) was used to analyze the ginseng samples. A total of 53 saponins were successfully identified, and the possible transformation pathways of several ginsenosides were described. Multivariate statistical analysis methods were used to perform the pattern recognition and further to select the marker compounds of samples. Twenty ginsenosides were considered to contribute most to the sample classification, six of which including Rg3, Rk1, Rh4, Rs3, Rs5, and Rk2 increased significantly in BG, while the other fourteen ginsenosides were greatly elevated in WG. The changes of ginsenoside in BG and WG were characterized by UHPLC-Q-Orbitrap-MS, which is of great significance for its quality control and effect evaluation.
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http://dx.doi.org/10.1080/14786419.2022.2062352DOI Listing
April 2022

Dynamic observation: Immune-privileged microenvironment limited the effectiveness of immunotherapy in an intraocular metastasis mouse model.

Ophthalmic Res 2022 Apr 8. Epub 2022 Apr 8.

Introduction: Intraocular metastasis (IM) occurred in approximately 8-10% of patients with metastatic malignancy, for whom oncological immunotherapies showed poor visual potential. However, the mechanism for that inefficiency remains unclear and requires further exploration.

Methods: we established a novel mouse model of intraocular metastasis by intracarotid injection of cutaneous melanoma cells. We investigated disease progression using ophthalmic and histological examinations. We used combined anti-PD-1 and anti-CTLA4 antibodies for immunotherapy and evaluate the therapeutic effects in mice model. In addition, we characterized the immune microenvironment of tumor infiltrating CD8+ T by fluorescence staining and assessed their cytotoxicity by flow cytometry.

Results: All mice presented IM in the left eye, while the right eye was healthy. Uveal tissues with rich vascularity (e.g., the iris, ciliary body, and choroid) initiated intraocular metastasis at an early stage, and intraocular metastasis development resulted in several secondary changes, including corneal swelling, retinal detachment, and intratumoral haemorrhage. Immunotherapy could inhibit intraocular metastasis, prolong the time to eye rupture but did not prevent rupture ending. This inefficiency might be attributed to ocular tissues specificities that inhibited CD8+ T cells infiltration via PD-L1 expression. PD-L1low corneal tissue resisted tumor invasion with high levels of CD8+ T cells infiltration, whereas CD8+ T cells were deficient in PD-L1high uveal metastasis. Furthermore, we found a significantly increased PD-1+/- CD4+ and PD-1+/- CD8+ T cells infiltrating the intratumoral haemorrhage area. Although these CD8+ T cells in the IM were not exhausted and had a higher capacity of cytotoxicity (higher Interferon-γ ratio) than CD8+ T cells in the blood, FasL+ PD-L1+ ocular tissue can strongly inhibit these IM infiltrating T cells.

Conclusions: Immunotherapy can inhibit the disease progression of intraocular metastasis. Enhancing the effects of tumor-infiltrating CD8+ T cells should be one of the highest potentials to improve the visual potential.
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http://dx.doi.org/10.1159/000524485DOI Listing
April 2022

Trial of transcutaneous electrical acupoint stimulation in laryngopharyngeal reflux disease: study protocol for a randomized controlled trial.

Trials 2022 Apr 8;23(1):272. Epub 2022 Apr 8.

Department of Otolaryngology-Head & Neck Surgery, The First Affiliated Hospital of Anhui Medical University, No. 81 Meishan Road, Shushan District, Hefei, Anhui, 230022, People's Republic of China.

Background: Patients with persistent globus sensation, throat clearing, chronic cough, hoarseness, and other throat symptoms account for a large proportion of patients in ears, nose, and throat clinics. Laryngopharyngeal reflux disease (LPRD) is increasingly valued by otolaryngologists. Transcutaneous electrical acupoint stimulation (TEAS) is possibly a new method for the treatment of LPRD. This trial aims to determine whether TEAS combined with proton pump inhibitor (PPI) is better than PPI alone in the treatment of LPRD.

Methods: This prospective randomized controlled trial will be implemented in a tertiary hospital in China. Seventy patients diagnosed with LPRD will be randomly assigned to the TEAS + PPI group (intervention group) or PPI group (control group), at a ratio of 1:1. In addition to using PPI, the intervention group will receive TEAS at four groups of acupoints, and each group will be treated for 15 min, once for 60 min, five times a week, for 12 weeks, 60 times. The main outcome will be changes in the Reflux Symptom Index scores at 4, 12, and 24 weeks after treatment. The secondary outcomes will include changes in the reflux finding score, Laryngopharyngeal Reflux-Health-related Quality of Life score, and throat pain visual analog scale score.

Discussion: This trial will explore the feasibility of TEAS combined with PPI for the treatment of LPRD and provide potential evidence for its effectiveness and safety. The results of this study will be published in a peer-reviewed journal.

Trial Registration: Chinese Clinical Trial Registry ChiCTR2100046755 . Registered on May 28, 2021.
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http://dx.doi.org/10.1186/s13063-022-06193-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8991573PMC
April 2022

In situ transient Laue x-ray diffraction during high strain-rate tension.

Rev Sci Instrum 2022 Mar;93(3):033902

Institute of High Energy Physics, Chinese Academy of Sciences, 19B Yuquan Road, Beijing 100049, China.

In situ transient synchrotron Laue x-ray diffraction based on high-energy and broadband x rays under high strain-rate tensile loading was developed at a superconducting wiggler beamline at the Beijing Synchrotron Radiation Facility. A split-Hopkinson tensile bar is utilized to realize this dynamic loading condition, while the transient Laue x-ray diffraction captures the transient internal structure of monocrystalline materials. Plastic deformation of a monocrystalline nickel specimen was investigated to prove the ability of this instrumentation in the characterization of a dynamic response of monocrystalline materials during a high strain-rate impact process with 5 µs time resolution.
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http://dx.doi.org/10.1063/5.0079582DOI Listing
March 2022

Enhancing the Cellular Uptake of Macromolecules via Enzyme-Instructed Self-Assembly.

Langmuir 2022 04 31;38(14):4364-4370. Epub 2022 Mar 31.

Bioinspired Soft Matter Unit, Okinawa Institute of Science and Technology, Okinawa 904-0495, Japan.

Poor solubility, low cellular uptake, and poor cell selectivity are the main obstacles hampering the therapeutic potential and clinic application of macromolecules. To overcome these limitations, here we propose a chemical modification strategy of macromolecules based on enzyme-instructed self-assembly (EISA). By using protoporphyrin IX (PpIX) and its metal complex Zn-PpIX as the modification objects, we demonstrated that the integration of enzymatic transformation and molecular self-assembly of macromolecules successfully improved the solubility of macromolecules, enhancing their intracellular uptake selectively against cancer cells. The proposed strategy is potentially applicable as a general tool for the development of macromolecule-based nanomedicine.
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http://dx.doi.org/10.1021/acs.langmuir.2c00101DOI Listing
April 2022

A visual portable microfluidic experimental device with multiple electric field regulation functions.

Lab Chip 2022 04 12;22(8):1556-1564. Epub 2022 Apr 12.

State Key Laboratory of Robotics and Systems, Harbin Institute of Technology, West Da-zhi Street 92, Harbin, Heilongjiang 150001, People's Republic of China.

High portability and miniaturization are two of the most important objectives pursued by microfluidic methods. However, there remain many challenges for the design of portable and visual microfluidic devices (, electrokinetic experiments) due to the use of a microscope and power supply. To this end, we report a visual portable microfluidic experimental device (PMED) with multiple electric field regulation functions, which can realize the electric field regulation functions of various basic microfluidic experiments through modular design. The internal reaction process of the microfluidic chip is displayed by a smartphone, and the experimental results are analyzed using a mobile phone application (APP). Taking the induced-charge electroosmosis (ICEO) particle focusing phenomenon as an example, we carried out detailed experiments on PMED and obtained conclusions consistent with numerical simulations. In addition to ICEO experiments, other functions such as alternating electroosmosis (ACEO), thermal buoyancy convection, and dielectrophoresis (DEP) can be realized by replacing module-specific covers. The device expands the application of microfluidic experiments and provides a certain reference for the further integration and portability of subsequent microfluidic experiment devices.
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http://dx.doi.org/10.1039/d2lc00152gDOI Listing
April 2022

Freeze-Thawing Treatment as a Simple Way to Tune the Gel Property and Digestibility of Minced Meat from Red Swamp Crayfish ().

Foods 2022 Mar 15;11(6). Epub 2022 Mar 15.

School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China.

The effects of freezing methods, including rapid freezing (RF) or slow freezing (SF), combined with thawing methods, e.g., water immersing thawing (WT) or cold thawing (CT), on the meat yield, drip loss, gel properties, and digestive properties of meat detached from red swamp crayfish were investigated. RF greatly reduced the freezing time compared to SF, and the thawing time of frozen crayfish was obviously shortened by WT in comparison to CT. RF and CT improved the meat yield but increased the drip loss, probably as a result of the greater protein denaturation or degradation. A soft and flexible gel was obtained by SF-CT, while a hard one was achieved by RF-WT. An SEM analysis showed that SF resulted in rough and irregular microstructures with larger pore sizes. Freeze-thawing led to an increase in the β-sheet content at the expense of α-helix and variations in the microenvironment of tyrosine and tryptophan residues in protein molecules of the gels, which was more pronounced in the SF-CT group. Moreover, freeze-thawing could cause enhanced protein digestibility but reduce the antioxidant activity of gels. These findings underline the promise of the freezing-thawing treatment in tuning the gel-based meat products of crayfish.
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http://dx.doi.org/10.3390/foods11060837DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8950141PMC
March 2022

Resonance-Induced Stimuli-Responsive Capacity Modulation of Organic Ultralong Room Temperature Phosphorescence.

J Am Chem Soc 2022 Apr 22;144(15):6946-6953. Epub 2022 Mar 22.

State Key Laboratory of Organic Electronics and Information Displays & Institute of Advanced Materials (IAM), Nanjing University of Posts & Telecommunications, 9 Wenyuan Road, Nanjing 210023, China.

Organic ultralong room temperature phosphorescence (OURTP) materials having stimuli-responsive attributes have attracted great attention due to their great potential in a wide variety of advanced applications. It is of fundamental importance but challengeable to develop stimuli-responsive OURTP materials, especially such materials with modulated optoelectronic properties in a controlled manner probably due to the lack of an authentic construction approach. Here, we propose an effective strategy for OURTP materials with controllably regulated stimuli-responsive properties by engineering the resonance linkage between flexible chain and phosphor units. A quantitative parameter to demonstrate the stimuli-responsive capacity is also established by the responsivity rate constant. The designed OURTP materials demonstrate efficient photoactivated OURTP with lifetimes up to 724 ms and tunable responsivity rate constants ranging from 0.132 to 0.308 min upon continuous UV irradiation. Moreover, the applications of stimuli-responsive resonance OURTP materials have been illustrated by the rewritable paper for snapshot and Morse code for multiple information encryption. Our works, which enable the accomplishment of OURTP materials capable of on-demand manipulated optical properties, demonstrate a viable design to explore smart OURTP materials, giving deep insights into the dynamically stimuli-responsive process.
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http://dx.doi.org/10.1021/jacs.2c01669DOI Listing
April 2022

Concurrent CDX2 cis-deregulation and UBTF-ATXN7L3 fusion define a novel high-risk subtype of B-cell ALL.

Blood 2022 Mar 22. Epub 2022 Mar 22.

Université de Paris, UMR 7212 / INSERM U944, France.

Oncogenic alterations underlying B-cell acute lymphoblastic leukemia (B-ALL) in adults remain incompletely elucidated. To uncover novel oncogenic drivers, we performed RNA-seq and whole-genome analyses in a large cohort of unresolved B-ALL. We identified a novel subtype characterized by a distinct gene expression signature and the unique association of two genomic microdeletions. The 17q21.31 microdeletion resulted in a UBTF::ATXN7L3 fusion transcript encoding a chimeric protein. The 13q12.2 deletion resulted in monoallelic ectopic expression of the homeobox transcription factor CDX2, located 138 kb in cis from the deletion. Using 4C-seq and CRISPR interference experiments, we elucidated the mechanism of CDX2 cis-deregulation, involving PAN3 enhancer hijacking. CDX2/UBTF ALL (n=26) harbored a distinct pattern of additional alterations including 1q gain and CXCR4 activating mutations. Within adult patients with Ph-negative B-ALL enrolled in GRAALL trials, patients with CDX2/UBTF ALL (n=17/723, 2.4%) were frequently young women (M/F ratio 0.2, P=0.002, median age 31 years). They commonly presented with a pro-B phenotype ALL and moderate blast cell infiltration. They had poor response to treatment including a higher risk of failure to first induction course (19% versus 3%, P=0.017) and higher post-induction MRD levels (MRD ≥ 10-4, 93%, versus 46%, P<0.001). This early resistance to treatment translated into a significantly higher cumulative incidence of relapse (75.0% versus 32.4%, P=0.004) in univariate and multivariate analyses. In conclusion, we discovered a novel B-ALL entity defined by the unique combination of CDX2 cis-deregulation and UBTF::ATXN7L3 fusion, representing a high-risk disease in young adults. Study can be found on https://clinicaltrials.gov NCT00327678 and NCT02617004.
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http://dx.doi.org/10.1182/blood.2021014723DOI Listing
March 2022

Fluid pumping by liquid metal droplet utilizing ac electric field.

Phys Rev E 2022 Feb;105(2-2):025102

State Key Laboratory of Robotics and System, Harbin Institute of Technology, Harbin 150001, China.

We report a unique phenomenon in which liquid metal droplets (LMDs) under a pure ac electric field pump fluid. Unlike the directional pumping that occurs upon reversing the electric field polarity under a dc signal, this phenomenon allows the direction of fluid motion to be switched by simply shifting the position of the LMD within the cylindrical chamber. The physical mechanism behind this phenomenon has been termed Marangoni flow, caused by nonlinear electrocapillary stress. Under the influence of a localized, asymmetric ac electric field, the polarizable surface of the position-offset LMD produces a net time-averaged interfacial tension gradient that scales with twice the field strength, and thus pumps fluid unidirectionally. However, the traditional linear RC circuit polarization model of the LMD/electrolyte interface fails to capture the correct pump-flow direction when the thickness of the LMD oxide skin is non-negligible compared to the Debye length. Therefore, we developed a physical description by treating the oxide layer as a distributed capacitance with variable thickness and connected with the electric double layer. The flow profile is visualized via microparticle imaging velocimetry, and excellent consistency is found with simulation results obtained from the proposed nonlinear model. Furthermore, we investigate the effects of relevant parameters on fluid pumping and discuss a special phenomenon that does not exist in dc control systems. To our knowledge, no previous work addresses LMDs in this manner and uses a zero-mean ac electric field to achieve stable, adjustable directional pumping of a low-conductivity solution.
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http://dx.doi.org/10.1103/PhysRevE.105.025102DOI Listing
February 2022

Intrahabitat Differences in Bacterial Communities Associated with in the Large Shallow Eutrophic Lake Taihu.

Appl Environ Microbiol 2022 04 14;88(7):e0232821. Epub 2022 Mar 14.

State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnologygrid.458478.2, Chinese Academy of Sciences, Nanjing, People's Republic of China.

The Asian clam Corbicula fluminea is a keystone zoobenthos in freshwater ecosystems. However, its associated microbiome is not well understood. We investigated the bacterial communities of this clam and its surrounding environment, including sediment and water simultaneously, in a large lake by means of 16S rRNA gene sequencing. Approximately two-thirds of the bacterial operational taxonomic units (OTUs) associated with clams were observed in the surrounding environment and mostly from particle-associated samples. The associated bacterial communities were site specific and more similar to environmental bacteria from the same site than those at other sites, suggesting a local environmental influence on host bacteria. However, the significant differences in bacterial diversities and compositions between the clam and the environment also indicated strong host selection pressure on bacteria from the surrounding environment. Bacteria affiliated with , , , , , , and were found to be significantly enriched in the clams in comparison to their local environment. Oligotyping analyses of the core-associated bacterial OTUs also demonstrated that most of the core OTUs had lower relative abundances and occurrence frequencies in environmental samples. The core bacterial OTUs were found to play an important role in maintaining the stability of the bacterial community network. These core bacteria included the two most abundant taxa and with the potential function of fermenting polysaccharides for assisting host clams in food digestion. Overall, we demonstrate that clam-associated bacteria were spatially dynamic and site specific, which were mainly structured both by local environments and host selection. The Asian clam is an important benthic clam in freshwater ecosystems due to its high population densities and high filtering efficiency for particulate organic matter. While the associated microbiota is believed to be vital for host living, our knowledge about the compositions, sources, and potential functions is still lacking. We found that C. fluminea offers a unique ecological niche for specific lake bacteria. We also observed high intrahabitat variation in the associated bacterial communities. Such variations were driven mainly by local environments, followed by host selection pressure. While the local microbes served as a source of the clam-associated bacteria, host selection resulted in enrichments of bacterial taxa with the potential for assisting the host in organic matter digestion. These results significantly advance our current understanding of the origins and ecological roles of the microbiota associated with a keynote clam in freshwater ecosystems.
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http://dx.doi.org/10.1128/aem.02328-21DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9004352PMC
April 2022
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