Publications by authors named "Tao Yang"

2,651 Publications

  • Page 1 of 1

Downregulation of miR-20b-5p Contributes to the Progression of Liver Fibrosis via the STAT3 Signaling Pathway In Vivo and In Vitro.

Dig Dis Sci 2022 Aug 10. Epub 2022 Aug 10.

Department of General Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an, China.

Background: Activated hepatic stellate cells (HSCs) are primarily involved in liver fibrosis and portal hypertension (PHT). We aimed to investigate the effect of miR-20b-5p on HSCs, liver fibrosis, and PHT.

Methods: MiR-20b-5p expression in HSCs and in mouse liver fibrosis was determined by qPCR. Further, the effects of miR-20b-5p mimic on HSCs migration, proliferation, and apoptosis were investigated in vitro. A dual-luciferase reporter assay was performed to confirm the direct interaction between miR-20b-5p and STAT3. In vivo, mouse liver fibrosis was established by common bile duct ligation and intervened by agomiR-20b-5p. Sirius red staining and hydroxyproline content were used to evaluate collagen deposition. The α-SMA expression in the liver was detected by IHC and Western blotting. The STAT3 signaling pathway and its downregulated cytokines as well as portal pressure and angiogenesis were explored.

Results: MiR-20b-5p was significantly downregulated during HSCs activation and in mouse liver fibrosis. The functional analyses demonstrated that miR-20b-5p inhibited cell proliferation, activation, and promoted apoptosis in HSCs in vitro. Moreover, miR-20b-5p regulated STAT3 expression by binding to the 3'UTR of its miRNA directly. Overexpression of miR-20b-5p facilitated HSC activation and proliferation by inhibiting the STAT3 signaling pathway. MiR-20b-5p overexpression suppressed the STAT3 and its downstream cytokines and ameliorated liver fibrosis in mice. The intra- and inter-hepatic angiogenesis were also effectively inhibited. The inhibition of liver fibrosis and neoangiogenesis contributed to the decrease of portal pressure.

Conclusions: MiR-20b-5p plays an important role in the fibrosis and angiogenesis of liver fibrosis by targeting the STAT3 signaling pathway.
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http://dx.doi.org/10.1007/s10620-022-07660-zDOI Listing
August 2022

Frequently Expressed Glypican-3 As A Promising Novel Therapeutic Target for Osteosarcomas.

Cancer Sci 2022 Aug 9. Epub 2022 Aug 9.

South China University of Technology School of Medicine, Guangzhou, China.

Osteosarcoma (OS) is the most common bone malignancy without a reliable therapeutic target. Glypican-3 (GPC3) mutation and upregulation have been detected in multi-drug resistant OS, and anti-GPC3 immunotherapy can effectively suppress the growth of organoids. Further profiling of GPC3 mutations and expression patterns in OS is of clinical significance. To address these issues, fresh OS specimens were collected from 24 patients for cancer-targeted next-generation sequencing (NGS) and three-dimensional patient-derived organoid (PDO) culture. A tumor microarray was prepared using 37 archived OS specimens. Immunohistochemical (IHC) staining was performed on OS specimens and microarrays to profile GPC3 and CD133 expression as well as intratumoral distribution patterns. RT-PCR was conducted to semi-quantify GPC3 and CD133 expression levels in the OS tissues. Anti-GPC3 immunotherapy was performed on OS organoids with or without GPC3 expression and its efficacy was analyzed using multiple experimental approaches. No OS cases with GPC3 mutations were found, except for the positive control (OS-08). IHC staining revealed GPC3 expression in 73.77% (45/61) of OSs in weak (+; 29/45), moderate (++; 8/45), and strong (+++; 8/45) immunolabeling densities. The intratumoral distribution of GPC3-positive cells was variable in the focal (+; 10-30%; 8/45), partial (++; 31-70%; 22/45), and the most positive patterns (+++; > 71%; 15/45), which coincided with CD133 immunolabeling (P = 9.89×10 ). The anti-GPC3 antibody efficiently inhibits Wnt/β-catenin signaling and induces apoptosis in GPC3-positive PDOs and PDXs, as opposed to GPC3-negative PDOs and PDXs. The high frequency of GPC3 and CD133 co-expression and the effectiveness of anti-wildtype GPC3-ab therapy in GPC3-positive OS models suggest that GPC3 is a novel prognostic parameter and a promising therapeutic target for osteosarcoma.
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http://dx.doi.org/10.1111/cas.15521DOI Listing
August 2022

The Relative Body Weight Gain From Early to Middle Life Adulthood Associated With Later Life Risk of Diabetes: A Nationwide Cohort Study.

Front Endocrinol (Lausanne) 2022 19;13:927067. Epub 2022 Jul 19.

Department of Endocrinology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.

Aim: To determine the effect of decade-based body weight gain from 20 to 50 years of age on later life diabetes risk.

Methods: 35,611 non-diabetic participants aged ≥ 50 years from a well-defined nationwide cohort were followed up for average of 3.6 years, with cardiovascular diseases and cancers at baseline were excluded. Body weight at 20, 30, 40, and 50 years was reported. The overall 30 years and each 10-year weight gain were calculated from the early and middle life. Cox regression models were used to estimate risks of incident diabetes.

Results: After 127,745.26 person-years of follow-up, 2,789 incident diabetes were identified (incidence rate, 2.18%) in 25,289 women (mean weight gain 20-50 years, 7.60 kg) and 10,322 men (7.93 kg). Each 10-kg weight gain over the 30 years was significantly associated with a 39.7% increased risk of incident diabetes (95% confidence interval [CI], 1.33-1.47); weight gain from 20-30 years showed a more prominent effect on the risk of developing diabetes before 60 years than that of after 60 years (Hazard ratio, HR = 1.084, 95% CI [1.049-1.121], 0.0001 vs. 1.015 [0.975-1.056], = 0.4643; =0.0293). It showed a stable effect of the three 10-year intervals weight gain on risk of diabetes after 60 years (HR=1.055, 1.038, 1.043, respectively, all < 0.0036).

Conclusions: The early life weight gain showed a more prominent effect on developing diabetes before 60 years than after 60 years; however, each-decade weight gain from 20 to 50 years showed a similar effect on risk developing diabetes after 60 years.
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http://dx.doi.org/10.3389/fendo.2022.927067DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343618PMC
August 2022

Evaluation of site-diversified, fully functionalized diazirine probes for chemical proteomic applications.

Chem Commun (Camb) 2022 Aug 4. Epub 2022 Aug 4.

Institute of Drug Discovery Technology, Ningbo University, Ningbo 315211, China.

A series of site-diversified, fully functionalized diazirine probes are constructed based on a scaffold shared by several marketed EGFR-targeted drugs. The integrated analysis of protein targets of the site-diversified probe toolkit not only unveils more complete target space and helps suggest false positive targets, but also reveals dynamic events of multi-domain target-ligand interaction.
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http://dx.doi.org/10.1039/d2cc03868dDOI Listing
August 2022

Characterization of a Novel RNA Virus Causing Massive Mortality in Yellow Catfish, Pelteobagrus fulvidraco, as an Emerging Genus in ().

Microbiol Spectr 2022 Aug 4:e0062422. Epub 2022 Aug 4.

Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, People's Republic of China.

An emerging disease in farmed yellow catfish () causing massive mortality broke out in 2020 in Hubei, China. Histopathological examination indicated significant changes in kidneys and spleens of diseased fish. Electron microscopy revealed large numbers of viral particles in the kidneys and spleens. These particles were spherical with a diameter of approximately 35 nm. By using RNA sequencing and rapid identification of cDNA ends, the full nucleotide sequence of the virus was identified. The viral genome comprises 7,432 bp and contains three open reading frames sharing no nucleotide sequence similarity with other viruses; however, the amino acid sequence partially matched that of the nonstructural (NS) proteins from viruses in the order . Combined with the phylogenetic analysis, the conserved amino acid motifs and the domains of the viral genome predict a genome order typical of a calicivirus. Therefore, this virus was tentatively named yellow catfish calicivirus (YcCV). Cell culture showed that YcCV could cause a cytopathic effect in the channel catfish kidney cell line (CCK) at early passages. In artificial infection, this virus could infect healthy yellow catfish and led to clinical symptoms similar to those that occurred naturally. hybridization analysis detected positive signals of the virus in kidney, spleen, liver, heart, and gill tissues of diseased fish. This study represents the first report of calicivirus infection in yellow catfish and provides a solid basis for future studies on the control of this viral disease. Caliciviruses are rapidly evolving viruses that cause pandemic outbreaks associated with significant morbidity and mortality globally. A novel calicivirus identified from yellow catfish also causes substantial mortality. Using an RNA sequencing (RNA-seq) and rapid amplification of cDNA ends (RACE) method, the full nucleotide sequence was identified and characterized, and this virus was tentatively named yellow catfish calicivirus (YcCV). A nucleotide sequence similarity search found no match with other viruses, and an amino acid sequence comparison indicated approximately 23.3% amino acid homology with the viruses in the order . These findings may represent a new avenue to explain virus evolution and suggest a need to further study the pathogenesis of calicivirus and characterize possible interactions among interspecific viruses in the aquaculture environment.
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http://dx.doi.org/10.1128/spectrum.00624-22DOI Listing
August 2022

Screening of cell-virus, cell-cell, gene-gene crosstalk among animal kingdom at single cell resolution.

Clin Transl Med 2022 Aug;12(8):e886

China National GeneBank, Shenzhen, China.

Background: The exact animal origin of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) remains obscure and understanding its host range is vital for preventing interspecies transmission.

Methods: Herein, we applied single-cell sequencing to multiple tissues of 20 species (30 data sets) and integrated them with public resources (45 data sets covering 26 species) to expand the virus receptor distribution investigation. While the binding affinity between virus and receptor is essential for viral infectivity, understanding the receptor distribution could predict the permissive organs and tissues when infection occurs.

Results: Based on the transcriptomic data, the expression profiles of receptor or associated entry factors for viruses capable of causing respiratory, blood, and brain diseases were described in detail. Conserved cellular connectomes and regulomes were also identified, revealing fundamental cell-cell and gene-gene cross-talks from reptiles to humans.

Conclusions: Overall, our study provides a resource of the single-cell atlas of the animal kingdom which could help to identify the potential host range and tissue tropism of viruses and reveal the host-virus co-evolution.
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http://dx.doi.org/10.1002/ctm2.886DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9345398PMC
August 2022

Asymmetric ultrathin silica nanonets as a super-performance emulsifier.

J Colloid Interface Sci 2022 Jul 22;628(Pt A):109-120. Epub 2022 Jul 22.

Liaoning Key Laboratory for Green Synthesis and Preparative Chemistry of Advanced Materials, College of Chemistry, Liaoning University, Shenyang 110036, China. Electronic address:

Hypothesis: Pickering emulsions have been used in many fields such as catalytic synthesis, pharmaceutics and oilfield chemicals. They usually have good stability, but in some extreme conditions such as at high temperatures or in special liquid-liquid systems, poor stability is often encountered.

Experiments: Herein, ultrathin silica nanosheets with controllable morphologies were synthesized via a simple interfacial anisotropic self-assembly approach integrated with pore-forming techniques. By regulating the size, density and pattern of the apertures, three types of unique nanosheets including mesoporous nanosheets, meso/macroporous topology-nanosheets and asymmetric nanonets with hollows were obtained.

Findings: After a simple hydrophobic modification, the nanonets exhibited super-performance as particulate emulsifiers, owing to their two-dimensional (2D) structures of large pore volume and hierarchical pore/hollow arrangements. As a result, those silica nanonets can stabilize various emulsion systems at considerably high temperatures that are difficult to be stabilized by conventional particulate emulsifiers even at a dose of 100x higher. This work paves a promising way to develop novel 2D asymmetric nanomaterials with tunable compositions, aperture parameters and morphologies for emulsification and potential applications.
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http://dx.doi.org/10.1016/j.jcis.2022.07.121DOI Listing
July 2022

Analysis of Research Status and Development Trend of Nanotoxicology of Liliaceae Medicinal Plants.

Biomed Res Int 2022 21;2022:9777817. Epub 2022 Jul 21.

College of Pharmacy, Gansu University of Chinese Medicine, Lanzhou 730030, China.

The research status and development trend of nanotoxicology of Liliaceae medicinal plants were analyzed. In the research, the toxicology of Liliaceae medicinal plants was investigated by the preparation method of silver nanoparticles. By means of spectral curve experiment, the present situation of nanotoxicology of Liliaceae medicinal plants was analyzed, and then its subsequent development trend was analyzed. In this process, Liliaceae medicinal plants could be used effectively, which could create great economic benefits. In the application of the above scheme, the toxicological degradation of Liliaceae medicinal plants could be controlled at about 96%. The high-dose silver nanoparticles could reach 100 M, and the silver nitrate could reach 10 or 30 M.
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http://dx.doi.org/10.1155/2022/9777817DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334102PMC
August 2022

Ultrasound enhanced butyric acid-lauric acid designer lipid synthesis: Based on artificial neural network and changes in enzymatic structure.

Ultrason Sonochem 2022 Jul 22;88:106100. Epub 2022 Jul 22.

State Key Lab of Food Science and Technology and Collaborative Innovation Center of Food Safety and Quality Control, Jiangnan University, Wuxi 214122, China. Electronic address:

Ultrasound is a green technology for intensifying enzymatic reactions. In this study, an ultrasonic water bath with equipment parameters of 28 kHz, 1750.1 W/m, 60% duty cycle was used to assist the synthesis of butyric acid-lauric acid designer lipid (BLDL), which was catalyzed by Lipozyme 435. A convincing three-layer feed-forward artificial neural network (ANN) model was established (R = 0.949, RMSE = 4.759, ADD = 7.329) to accurately predict the optimal parameters combination, which was described as 13.72 mL reaction volume, 15.49% enzyme loading, 0.253 substrate molar ratio (tributyrin/lauric acid), 56.58 °C reaction temperature and 120 min reaction time. The ultrasonic assistance increased actual butyric acid conversion rate by 11.38%, and also enhanced the consumption rate of tributyrin and lauric acid during the reaction. Meanwhile, the esterification activity of Lipozyme 435 was enhanced and its effectiveness up to 6 cycles. Structurally, ultrasound assistance significantly disrupted the secondary structure of the Lipozyme 435: reduced the content of α-helices, increased the content of β-sheet and β-turn. In addition, sonication caused an increase in crevice and micro-damage on the surface of the immobilized enzyme. In conclusion, low-intensity ultrasound at 28 kHz improved the synthesis efficiency of BLDL, which was scientifically predicted by ANN model, and the change of enzyme structure may be the vital reason for ultrasound enhanced reaction. However, the effect of ultrasound on immobilized enzymes' activity needs to be further explored.
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http://dx.doi.org/10.1016/j.ultsonch.2022.106100DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340510PMC
July 2022

Mobile Health Applications for Depression in China: A Systematic Review.

Cureus 2022 Jul 26;14(7):e27299. Epub 2022 Jul 26.

Psychiatry, University of British Columbia, Vancouver, CAN.

Mobile health (mHealth) applications (apps) have the potential to increase access to mental health care. In China, there is growing interest in mHealth apps for depression. Our objective was to systematically review research on mHealth for depression in China to identify benefits and challenges. A systematic literature search was conducted using Chinese and English databases in accordance with Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Randomized and nonrandomized clinical studies on mHealth apps and depression in China were included. Study quality was assessed using the Cochrane Risk of Bias tool. Seven studies met the inclusion criteria with three randomized trials, two quasi-randomized trials, one clinical trial with an uncertain grouping method, and one study with a single-group design. All studies used the WeChat platform and included activities such as psychoeducation, self-management, supervised group chats, and/or remote contact with a healthcare team, in comparison to usual care. All studies reported significant and large benefits for outcomes, but the risk of bias was high. There are few rigorous evaluations of mHealth apps for depression in China, with all included studies involving WeChat programs and most using WeChat to extend nursing discharge care for inpatients with depression. While these studies showed significant improvement in health outcomes as compared to usual care, the results remain inconclusive because of the high risk of bias. mHealth holds promise for increasing access to mental health care in China, but issues such as efficacy, scalability, patient and clinician acceptability, and data privacy must be addressed.
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http://dx.doi.org/10.7759/cureus.27299DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320610PMC
July 2022

Novel solar-driven ferrate(VI) activation system for micropollutant degradation: Elucidating the role of Fe(IV) and Fe(V).

J Hazard Mater 2022 09 22;437:129428. Epub 2022 Jun 22.

State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China.

This paper presents a novel process of solar-ferrate(VI) [Fe(VI)] for micropollutant degradation. The solar-Fe(VI) process promoted micropollutant degradation compared with Fe(VI) alone and solar. The radical scavenging and probing experiment results suggested that Fe(V) and Fe(IV) but not reactive oxygen species were most likely involved in the solar-Fe(VI) process. Through building a kinetic model, Fe(IV) and Fe(V) were observed to play an equally significant role in the solar-Fe(VI) process. Afterward, the reaction mechanism of the photochemistry of Fe(VI) was elaborated. Fe(IV) formed from Fe(VI) photolysis and then decomposed to Fe(II) which reacted with Fe(VI) to form Fe(V). Furthermore, the effect of pH on carbamazepine (CBZ) degradation was studied and the quantum yields of Fe(VI) were determined, with (1.98 ± 0.16)× 10 mol∙einstein, (5.90 ± 0.27)× 10 mol∙einstein, and (1.66 ± 0.14)× 10 mol∙einstein at pH 7.0, 8.0, and 9.0, respectively. Inorganic ions, including Cl, HCO, and Br displayed negligible influence on the CBZ degradation, whereas humic acid inhibited the CBZ degradation. Finally, the solar-Fe(VI) process exhibited good applicability in authentic waters and under different irradiation (natural sunlight, ultraviolet light, and visible light from solar cut-off emission). Overall, this study provides a new routine for efficient micropollutant elimination and reveals the photochemistry of Fe(VI).
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http://dx.doi.org/10.1016/j.jhazmat.2022.129428DOI Listing
September 2022

Semiglobal Suboptimal Output Regulation for Heterogeneous Multi-Agent Systems With Input Saturation via Adaptive Dynamic Programming.

IEEE Trans Neural Netw Learn Syst 2022 Jul 27;PP. Epub 2022 Jul 27.

This article considers the semiglobal cooperative suboptimal output regulation problem of heterogeneous multi-agent systems with unknown agent dynamics in the presence of input saturation. To solve the problem, we develop distributed suboptimal control strategies from two perspectives, namely, model-based and data-driven. For the model-based case, we design a suboptimal control strategy by using the low-gain technique and output regulation theory. Moreover, when the agents' dynamics are unknown, we design a data-driven algorithm to solve the problem. We show that proposed control strategies ensure each agent's output gradually follows the reference signal and achieves interference suppression while guaranteeing closed-loop stability. The theoretical results are illustrated by a numerical simulation example.
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http://dx.doi.org/10.1109/TNNLS.2022.3191673DOI Listing
July 2022

Shock Acceleration and Attenuation during Running with Minimalist and Maximalist Shoes: A Time- and Frequency-Domain Analysis of Tibial Acceleration.

Bioengineering (Basel) 2022 Jul 16;9(7). Epub 2022 Jul 16.

Auckland Bioengineering Institute, The University of Auckland, Auckland 1010, New Zealand.

Tibial shock attenuation is part of the mechanism that maintains human body stabilization during running. It is crucial to understand how shock characteristics transfer from the distal to proximal joint in the lower limb. This study aims to investigate the shock acceleration and attenuation among maximalist shoes (MAXs), minimalist shoes (MINs), and conventional running shoes (CONs) in time and frequency domains. Time-domain parameters included time to peak acceleration and peak resultant acceleration, and frequency-domain parameters contained lower (3-8 Hz) and higher (9-20 Hz) frequency power spectral density (PSD) and shock attenuation. Compared with CON and MAX conditions, MINs significantly increased the peak impact acceleration of the distal tibia ( = 0.01 and < 0.01). Shock attenuation in the lower frequency depicted no difference but was greater in the MAXs in the higher frequency compared with the MIN condition ( < 0.01). MINs did not affect the tibial shock in both time and frequency domains at the proximal tibia. These findings may provide tibial shock information for choosing running shoes and preventing tibial stress injuries.
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http://dx.doi.org/10.3390/bioengineering9070322DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9312333PMC
July 2022

Penta-BeP Monolayer: A Superior Sensor for Detecting Toxic Gases in the Air with Excellent Sensitivity, Selectivity, and Reversibility.

ACS Appl Mater Interfaces 2022 Aug 25;14(30):35229-35236. Epub 2022 Jul 25.

Laboratory of High Pressure Physics and Material Science (HPPMS), School of Physics and Physical Engineering, Qufu Normal University, Qufu, Shandong 273165, China.

Directly and quickly detecting toxic gases in the air is urgently needed in industrial production and our daily life. However, the poor gas selectivity and low sensitivity under ambient conditions limit the development of gas sensors. In this work, we demonstrate that the penta-BeP monolayer is an excellent toxic gas sensor by using first-principles calculations. The calculated results show that the semiconducting penta-BeP monolayer can chemisorb toxic gas molecules (including CO, NH, NO, and NO) with distinct charge transfer (-0.182 to 1.129 e) but negligibly interact with ambient gas molecules (including H, N, HO, O, and CO), indicating high gas selectivity for primary environmental gases. The calculated curves show that the penta-BeP monolayer exhibits a fast response with toxic gas molecules. Upon interaction with CO, NH, NO, and NO molecules at a bias voltage of 0.7 V, the currents are 10.23, 14.48, 32.10, and 129.90 times that of the pristine penta-BeP monolayer, respectively, which induces high sensitivity values of 9.23, 13.48, 31.10, and 128.90, respectively. Moreover, the moderate adsorption energies of all toxic gas molecules guarantee that the penta-BeP monolayer possesses good reversibility at room temperature with a short recovery time. Herein, all of our results indicate that the penta-BeP monolayer could be a superior candidate for sensing toxic gases with high selectivity, sensitivity, and reversibility.
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http://dx.doi.org/10.1021/acsami.2c07482DOI Listing
August 2022

Design of Personalized Ideological and Political Education Resource Recommendation System in the Online Education Platform in the Network Environment.

J Environ Public Health 2022 13;2022:3845749. Epub 2022 Jul 13.

Department of Intelligent Manufacturing, Hebei Vocational University of Industry and Technology, Shijiazhuang 050091, Hebei, China.

In the network environment, online ideological and political education is facing many challenges, the mainstream values of education have been impacted, and the backward educational model, single communication method, and solidified educational content are not conducive to the smooth progress of ideological and political education. However, at the same time, its innovation also ushers in development opportunities, rich educational resources, information platforms, diversified education subjects, strongly targeted education models, etc., which can become favorable factors for educational innovation. To this end, online educators should seize the development opportunities, grasp the good network environment, actively build a network platform for ideological and political education, create a personalized ideological and political education resource recommendation system, and reform the ideological and political education classroom, effectively playing the role of the main position of educating people. Online education breaks through the limitations of time and space, allowing students to get guidance from online teachers without leaving home, and is favored by more and more primary and secondary school students because of its unique advantages. At present, the forms of tutoring for off-campus online education in China are diverse, and there are several specific teaching modes. Whether online teaching can meet the needs of users is a problem worth studying.
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http://dx.doi.org/10.1155/2022/3845749DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9300294PMC
July 2022

Retractorless Surgery for Petroclival Meningiomas via the Subtemporal Approach: A Try to Reduce Brain Retraction Injury.

Comput Math Methods Med 2022 14;2022:6436542. Epub 2022 Jul 14.

Department of Neurosurgery, The First Affiliated Hospital of USTC, Division of Life Science and Medicine, University of Science and Technology of China, Hefei, Anhui 230036, China.

Purpose: To present our experience with retractorless surgery for resection of petroclival meningiomas (PCMs) via the subtemporal approach with routine operative instruments.

Methods: Clinical data of patients with PCMs who received surgical treatments via subtemporal approach were retrospectively analyzed. Patient demographics, duration of operation, extent of resection, postoperative brain injury rate, postoperative complication, and surgical outcome were reviewed.

Results: Twenty-nine consecutive patients with retractorless surgery via subtemporal approach performed between November 2018 and November 2021. The gross total resection rate was 82.8% ( = 24). The incidence of postoperative temporal lobe injury was 3.4% ( = 1). All the procedures were completed without fixed retraction or other specialized instruments.

Conclusions: Retractorless surgery via subtemporal approach is a reliable treatment option for PCMs, which can be completed with routine operative instruments.
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http://dx.doi.org/10.1155/2022/6436542DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9303107PMC
July 2022

Study on the Anti-demyelination Mechanism of Bu-Shen-Yi-Sui Capsule in the Central Nervous System Based on Network Pharmacology and Experimental Verification.

Mediators Inflamm 2022 12;2022:9241261. Epub 2022 Jul 12.

School of Traditional Chinese Medicine, Beijing Key Lab of TCM Collateral Disease Theory Research, Capital Medical University, Beijing 100069, China.

Methods: The potential active ingredients and corresponding potential targets of BSYS Capsule were obtained from the TCMSP, BATMAN-TCM, Swiss Target Prediction platform, and literature research. Disease targets of CNSD were explored through the GeneCards and the DisGeNET databases. The matching targets of BSYS in CNSD were identified from a Venn diagram. The protein-protein interaction (PPI) network was constructed using bioinformatics methods. Gene Ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed to predict the mechanisms of BSYS. Furthermore, the neuroprotective effects of BSYS were evaluated using a cell model of hydrogen peroxide- (HO-) induced cell death in OLN-93 cells.

Results: A total of 59 potential bioactive components of BSYS Capsule and 227 intersection targets were obtained. Topological analysis showed that AKT had the highest connectivity degrees in the PPI network. Enrichment analysis revealed that the targets of BSYS in the treatment of CNSD were the PI3K-Akt and MAPK signaling pathway, among other pathways. GO analysis results showed that the targets were associated with various biological processes, including apoptosis, reactive oxygen species metabolic process, and response to oxidative stress, among others. The experimental results demonstrated that BSYS drug-containing serum alleviated the HO-induced increase in LDH, MDA, and ROS levels and reversed the decrease in SOD and mitochondrial membrane potential induced by HO. BSYS treatment also decreased the number of TUNEL (+) cells, downregulated Bcl-2 expression, and upregulated Bax and c-caspase-3 expression by promoting Akt phosphorylation.

Conclusion: BSYS Capsule alleviated HO-induced OLN-93 cell injury by increasing Akt phosphorylation to suppress oxidative stress and cell apoptosis. Therefore, BSYS can be potentially used for CNSD treatment. However, the results of this study are only derived from in vitro experiments, lacking the validation of in vivo animal models, which is a limitation of our study. We will further verify the underlying mechanisms of BSYS in animal experiments in the future.
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http://dx.doi.org/10.1155/2022/9241261DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9296285PMC
July 2022

Identification and Characterization of Long Non-coding RNA in Tomato Roots Under Salt Stress.

Front Plant Sci 2022 4;13:834027. Epub 2022 Jul 4.

Institute of Horticulture Crops, Xinjiang Academy of Agricultural Sciences, Urumqi, China.

As one of the most important vegetable crops in the world, the production of tomatoes was restricted by salt stress. Therefore, it is of great interest to analyze the salt stress tolerance genes. As the non-coding RNAs (ncRNAs) with a length of more than 200 nucleotides, long non-coding RNAs (lncRNAs) lack the ability of protein-coding, but they can play crucial roles in plant development and response to abiotic stresses by regulating gene expression. Nevertheless, there are few studies on the roles of salt-induced lncRNAs in tomatoes. Therefore, we selected wild tomato () and cultivated tomato M82 to be materials. By high-throughput sequencing, 1,044 putative lncRNAs were identified here. Among them, 154 and 137 lncRNAs were differentially expressed in M82 and , respectively. Through functional analysis of target genes of differentially expressed lncRNAs (DE-lncRNAs), some genes were found to respond positively to salt stress by participating in abscisic acid (ABA) signaling pathway, brassinosteroid (BR) signaling pathway, ethylene (ETH) signaling pathway, and anti-oxidation process. We also construct a salt-induced lncRNA-mRNA co-expression network to dissect the putative mechanisms of high salt tolerance in . We analyze the function of salt-induced lncRNAs in tomato roots at the genome-wide levels for the first time. These results will contribute to understanding the molecular mechanisms of salt tolerance in tomatoes from the perspective of lncRNAs.
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http://dx.doi.org/10.3389/fpls.2022.834027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9295719PMC
July 2022

Resistance to Antihypertensive Drugs: Is Gut Microbiota the Missing Link?

Hypertension 2022 Jul 20:101161HYPERTENSIONAHA12219826. Epub 2022 Jul 20.

Department of Physiology and Pharmacology, UT Microbiome Consortium, Center for Hypertension and Precision Medicine, College of Medicine and Life Sciences, University of Toledo, OH.

Microbiota colonization begins at birth and continuously reshapes throughout the course of our lives, resulting in tremendous interindividual heterogeneity. Given that the gut microbiome, similar to the liver, houses many categories of catalytic enzymes, there is significant value in understanding drug-bacteria interactions. The discovery of this link could enhance the therapeutic value of drugs that would otherwise have a limited or perhaps detrimental effect on patients. Resistant hypertension is one such subset of the hypertensive population that poorly responds to antihypertensive medications, resulting in an increased risk for chronic cardiovascular illnesses and its debilitating effects that ultimately have a detrimental impact on patient quality of life. We recently demonstrated that the gut microbiota is involved in the metabolism of antihypertensive drugs and thus contributes to the pathophysiology of resistant hypertension. Due to a lack of knowledge of the mechanisms, novel therapeutic approaches that account for the gut microbiota may allow for better therapeutic outcomes in resistant hypertension. Therefore, the purpose of this review is to summarize our current, albeit limited, understanding of how the gut microbiota may possess particular enzymatic activities that influence the efficacy of antihypertensive drugs.
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http://dx.doi.org/10.1161/HYPERTENSIONAHA.122.19826DOI Listing
July 2022

Exploitation of Synchrotron Radiation Photoionization Mass Spectrometry in the Analysis of Complex Organics in Interstellar Model Ices.

J Phys Chem Lett 2022 Aug 21;13(30):6875-6882. Epub 2022 Jul 21.

Department of Chemistry, University of Hawai'i at Ma̅noa, 2545 McCarthy Mall, Honolulu, Hawaii 96822, United States.

Unravelling the generation of complex organic molecules (COMs) on interstellar nanoparticles (grains) is essential in establishing predictive astrochemical reaction networks and recognizing evolution stages of molecular clouds and star-forming regions. The formation of COMs has been associated with the irradiation of interstellar ices by ultraviolet photons and galactic cosmic rays. Herein, we pioneer the first incorporation of (SVUV-PI-ReTOF-MS) in laboratory astrophysics simulation experiments to afford an isomer-selective identification of key COMs (ketene (HC═CO); acetaldehyde (CHCHO); vinyl alcohol (HC═CHOH)) based on photoionization efficiency (PIE) curves of molecules desorbing from exposed carbon monoxide-methane (CO-CH) ices. Our results demonstrate that the SVUV-PI-ReTOF-MS approach represents a versatile, rapid methodology for a comprehensive identification and explicit understanding of the complex organics produced in space simulation experiments. This methodology is expected to significantly improve the predictive nature of astrochemical models of complex organic molecules formed abiotically in deep space, including biorelated species linked to the origins-of-life topic.
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http://dx.doi.org/10.1021/acs.jpclett.2c01628DOI Listing
August 2022

Early identification and severity prediction of acute respiratory infection (ESAR): a study protocol for a randomized controlled trial.

BMC Infect Dis 2022 Jul 20;22(1):632. Epub 2022 Jul 20.

Department of Infectious Diseases, Shanghai Key Laboratory of Infectious Diseases and Biosafety Emergency Response, National Medical Center for Infectious Diseases, Huashan Hospital, Fudan University, No. 12 Middle Urumqi Road, Jing'an, Shanghai, 200040, China.

Background: The outbreak of SARS-CoV-2 at the end of 2019 sounded the alarm for early inspection on acute respiratory infection (ARI). However, diagnosis pathway of ARI has still not reached a consensus and its impact on prognosis needs to be further explored.

Methods: ESAR is a multicenter, open-label, randomized controlled, non-inferiority clinical trial on evaluating the diagnosis performance and its impact on prognosis of ARI between mNGS and multiplex PCR. Enrolled patients will be divided into two groups with a ratio of 1:1. Group I will be directly tested by mNGS. Group II will firstly receive multiplex PCR, then mNGS in patients with severe infection if multiplex PCR is negative or inconsistent with clinical manifestations. All patients will be followed up every 7 days for 28 days. The primary endpoint is time to initiate targeted treatment. Secondary endpoints include incidence of significant events (oxygen inhalation, mechanical ventilation, etc.), clinical remission rate, and hospitalization length. A total of 440 participants will be enrolled in both groups.

Discussion: ESAR compares the efficacy of different diagnostic strategies and their impact on treatment outcomes in ARI, which is of great significance to make precise diagnosis, balance clinical resources and demands, and ultimately optimize clinical diagnosis pathways and treatment strategies. Trial registration Clinicaltrial.gov, NCT04955756, Registered on July 9th 2021.
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http://dx.doi.org/10.1186/s12879-022-07552-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9296892PMC
July 2022

Rotavirus and Norovirus Infections in Children Under 5 Years Old with Acute Gastroenteritis in Southwestern China, 2018-2020.

J Epidemiol Glob Health 2022 Jul 20. Epub 2022 Jul 20.

Yunnan Key Laboratory of Vaccine Research & Development On Severe Infectious Disease, Department of Immunogenetics, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, 650118, Yunnan, People's Republic of China.

Objective: Rotaviruses and noroviruses are important causes of acute gastroenteritis in children. While previous studies in China have mainly focused on rotavirus, we investigated the incidence of norovirus in addition to rotavirus in Southwestern China.

Methods: From January 2018 to December 2020, cases of rotavirus or norovirus infections among children under five ages with acute gastroenteritis were evaluated retrospectively.

Results: The detection rate of rotavirus was 24.5% (27,237/111,070) and norovirus was 26.1% (4649/17,797). Among 17,113 cases submitted for dual testing of both rotavirus and norovirus, mixed rotavirus/norovirus infections were detected in 5.0% (859/17,113) of cases. While there was no difference in norovirus incidence in outpatient compared to hospitalized cases, rotavirus was detected two times more in outpatients compared to hospitalized cases (26.6% vs.13.6%; P < 0.001). Both rotavirus and norovirus infections peaked in children aged 12-18 months seeking medical care with acute gastroenteritis (35.6% rotavirus cases; 8439/23,728 and 32.5% norovirus cases; 1660/5107). Rotavirus infections were frequent between December and March of each year while norovirus was detected earlier from October to December. Our results showed significant correlation between virus detection and environmental factors such as average monthly temperature but not relative humidity. In addition, we observed a reduction in the detection rates of rotavirus and norovirus at the beginning of the SARS-CoV-2 pandemic in 2020.

Conclusion: Our results indicate that rotavirus and norovirus are still important viral agents in pediatric acute gastroenteritis in Southwestern China.
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http://dx.doi.org/10.1007/s44197-022-00050-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9297278PMC
July 2022

Overview of the immunological mechanism underlying severe fever with thrombocytopenia syndrome (Review).

Int J Mol Med 2022 Sep 20;50(3). Epub 2022 Jul 20.

Department of Infectious Disease, The First Hospital Affiliated with Nanjing Medical University, Jiangsu Province Hospital, Nanjing, Jiangsu 210029, P.R. China.

Severe fever with thrombocytopenia syndrome (SFTS) has been acknowledged as an emerging infectious disease that is caused by the SFTS virus (SFTSV). The main clinical features of SFTS on presentation include fever, thrombocytopenia, leukocytopenia and gastrointestinal symptoms. The mortality rate is estimated to range between 5‑30% in East Asia. However, SFTSV infection is increasing on an annual basis globally and is becoming a public health problem. The transmission cycle of SFTSV remains poorly understood, which is compounded by the pathogenesis of SFTS not being fully elucidated. Since the mechanism underlying the host immune response towards SFTSV is also unclear, there are no effective vaccines or specific therapeutic agents against SFTS, with supportive care being the only realistic option. Therefore, it is now crucial to understand all aspects of the host‑virus interaction following SFTSV infection, including the antiviral states and viral evasion mechanisms. In the present review, recent research progress into the possible host immune responses against SFTSV was summarized, which may be useful in designing novel therapeutics against SFTS.
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http://dx.doi.org/10.3892/ijmm.2022.5174DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9333902PMC
September 2022

Can preoperative ureteral stents reduce the incidence of ureteral stricture after radiotherapy in patients with cervical cancer?

BMC Urol 2022 Jul 18;22(1):106. Epub 2022 Jul 18.

Department of Urology, Hebei General Hospital, Shijiazhuang, China.

Objective: To determine the impact of preoperative stent placement on postradiotherapy stricture rate in patients with cervical cancer after radical resection.

Methods: This study was a retrospective analysis of data collected from 55 cervical cancer patients treated with radiotherapy between June 2016 and June 2020. Patients were divided into the stent and control groups. After 3 months, the stricture rate and the complications related to stent placement between the two groups were compared.

Results: There were 12 (46.2%) and 10 (34.5%) cases of ureteral stricture in the stent (n = 26) and control (n = 29) groups, respectively, three months after the end of radiotherapy. The incidence rates of ureter stricture in the two groups were not significantly different (P = 0.378). Moreover, there were 20 units (38.5%) and 15 units (25.9%) ureteral strictures in the stent and control groups, respectively. No significant difference in the incidence rates of ureteral strictures was found between the two groups (P = 0.157). There were 13 (50.0%) and 10 (34.5%) cases of ureteral stricture in the stent (n = 26) and control (n = 29) groups, respectively, six months after the end of the radiotherapy. The incidence rates of ureter stricture in the two groups were not significantly different (P = 0.244). Moreover, there were 21 units (40.4%) and 15 units (25.9%) ureteral strictures in the stent and control groups, respectively. No significant difference in the incidence rates of ureteral strictures was found between the two groups (P = 0.105). Complications related to stent placement such as urinary tract infections and bladder irritation were statistically significant (P = 0.006 and P = 0.036) between the two groups; while the other complications were not significantly different (P = 0.070, P = 0.092 and P = 0.586).

Conclusions: Ureteral stents may not reduce the incidence of ureteral stricture after radiotherapy in patients with cervical cancer. The stent needs to be replaced regularly, and the complications related to stent placement may occur at any time. Thus, preoperative stent placement should be cautious for the clinical management of cervical cancer patients treated with postoperative radiotherapy.
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http://dx.doi.org/10.1186/s12894-022-01029-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9295481PMC
July 2022

Rs864745 in , an Islet Function Associated Variant, Correlates With Plasma Lipid Levels in Both Type 1 and Type 2 Diabetes Status, but Not Healthy Subjects.

Front Endocrinol (Lausanne) 2022 1;13:898893. Epub 2022 Jul 1.

Department of Endocrinology and Metabolism, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Objective: This study aims to reveal the association between rs864745 A>G variant and type 2 diabetes (T2D), type 1 diabetes (T1D) risk, and their correlation with clinical features, including islet function, islet autoimmunity, and plasma lipid levels.

Methods: We included 2505 healthy controls based on oral glucose tolerance test (OGTT), 1736 unrelated T2D, and 1003 unrelated autoantibody-positive T1D individuals. Binary logistic regression was performed to evaluate the relationships between rs864745 in and T2D, T1D, and islet-specific autoantibody status under the additive model, while multiple linear regression was used to assess its effect on glycemic-related quantitative traits and plasma lipid levels.

Results: We did not find any association between rs864745 in and T2D, T1D, or their subgroups (All P > 0.05). For glycemic traits, we found that the G allele of this variant was significantly associated with higher 120 min insulin level, insulinogenic index (IGI), corrected insulin response (CIR), and acute insulin response (BIGTT-AIR) (P = 0.033, 0.006, 0.009, and 0.016, respectively) in healthy individuals. Similar associations were observed in newly diagnosed T2D but not T1D individuals. Although this variant had no impact on islet autoimmunity (All P > 0.05), significant associations with plasma total cholesterol (TC) and low-density lipoprotein (LDL) level stratified by rs864745 variant were observed in the disease status of T2D (P = 0.002 and 0.003) and T1D (P = 0.024 and 0.009), with significant heterogeneity to healthy individuals.

Conclusions: The common rs864745 variant contributes to islet function and lipid metabolism, which might be put into genetic risk scores to assess the risk of related clinical features.
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http://dx.doi.org/10.3389/fendo.2022.898893DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9283698PMC
July 2022

Identification of six novel variants from nine Chinese families with hypophosphatemic rickets.

BMC Med Genomics 2022 07 16;15(1):161. Epub 2022 Jul 16.

Department of Medical Genetics, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, Beijing, 100005, China.

Background: Hypophosphatemic rickets (HR) is a rare genetic disorder associated with renal phosphate wasting and characterized by bone defects. Inactivating mutations in the phosphate regulating endopeptidase homolog X‑linked gene (PHEX) account for most cases of HR. The aim of this study was to identify causative variants in nine unrelated Chinese families associated with HR, and to determine potential pathogenicity of the identified variants.

Methods: Genomic DNA was isolated from the peripheral blood of HR patients and their healthy relatives, followed by next-generation sequencing and/or Sanger sequencing. In silico prediction combined with conservation analysis was performed to assess the effects of the variants, and 3D protein modeling was conducted to predict the functional effects on the encoded protein.

Results: All HR patients recruited in this study displayed bone deformities and tooth agenesis, as well as reduced serum phosphate levels and elevated urine phosphate levels. Nine PHEX variants were identified in eight families, including four novel variants (c.1661_1726del, c.980A > G, c.1078A > T, and c.1017_1051dup). Of the nine identified PHEX variants, five caused a truncated protein, two caused an altered amino acid, and the other two were the canonical splicing variants. Novel variants c.1336G > A and c.1364 T > C in SLC34A3 were also found in one family. Conservation analysis showed that all the amino acids corresponding to the missense variants were highly conserved. In silico analysis and 3D protein structure modeling confirmed the pathogenicity of these variants.

Conclusions: This study identified four novel variants in PHEX and two novel variants in SLC34A3 in a Chinese cohort with HR. Our findings highlight the dominant role of PHEX in HR, and expand the genotypic and phenotypic spectra of this disorder.
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http://dx.doi.org/10.1186/s12920-022-01305-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9287957PMC
July 2022

Can digestate recirculation promote biohythane production from two-stage co-digestion of rice straw and pig manure?

J Environ Manage 2022 Jul 12;319:115655. Epub 2022 Jul 12.

Key Laboratory of Dongting Lake Aquatic Eco-Environmental Control and Restoration of Hunan Province, School of Hydraulic Engineering, Changsha University of Science & Technology, Changsha, 410004, China; Key Laboratory of Water-Sediment Sciences and Water Disaster Prevention of Hunan Province, Changsha, 410114, China. Electronic address:

Digestate recirculation is often considered an important way to improve system stability (system acidification, ammonia inhibition, hydrolysis limitations, etc.) and gas production performance. However, it is not clear how the promotion of biohythane production works in anaerobic co-digestion with digestate recirculation of rice straw (RS) and pig manure (PM). Two sets of laboratory-scale two-stage continuous stirred tank reactors were operated continuously for 95 d to investigate the performance of biohythane production in the first/second phase under mesophilic (M)/thermophilic (T) and digestate recirculation conditions. Firstly, biohythane was not produced by PM with RS under digestate recirculation. The main reasons were: 1) Digestive recirculation promoted the growth of hydrogenotrophic methanogenic bacteria; and 2) limitations in hydrolysis. Secondly, digestate recirculation has positive effects on the removal rates (removal rates of TS, VS, polysaccharide, protein and TCOD increased by 30.4%, 22.3%, 9.9%, 31.4%, and 11.9%, respectively) and energy yield (up to 68.7%). Finally, there was a higher abundance of hydrogen-producing bacteria (Fervidobacterium [44.9%] and Coprothermobacter [18.8%]) in T2, accounting for >80% of the total, and of which the huge hydrogen production potential cannot be ignored. The results provide new ideas for alleviating the energy crisis and developing green energy in the future.
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http://dx.doi.org/10.1016/j.jenvman.2022.115655DOI Listing
July 2022

Feedback based on health advice via tracing bracelet and smartphone in the management of blood pressure among hypertensive patients: A community-based RCT trial in Chongqing, China.

Medicine (Baltimore) 2022 Jul 15;101(28):e29346. Epub 2022 Jul 15.

Yangtze Normal University, Chongqing, China.

Background: Lifestyle interventions have been recognized as a line of treatment for noncommunicable diseases. This study aimed to evaluate a bespoke mHealth approach to deliver personalized feedback to improve blood pressure and weight for hypertensive patients in community settings.

Methods: A total of 307 participants, 50 from each community, were expected to be in the intervention or control group. A professional health facilitator was assigned for each of the 6 communities. The primary outcomes of the study are the reduction in blood pressure and weight at baseline and postintervention.

Results: Of 307 recruited, 192 participants (62.5%) completed the study (intervention: 104 and control: 88). There was no difference in attrition rates between the 2 groups (33.5% vs 41.9%, P = .291). After 6 month of intensive feedback intervention through the mHealth approach, patients had better blood pressure (133.4 ± 15.3 vs 139.7 ± 16.0) compared with control.

Conclusions: People who were adherent to the intervention demonstrated a clinical benefit in weight and blood pressure.
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http://dx.doi.org/10.1097/MD.0000000000029346DOI Listing
July 2022

DEB-IBM for predicting climate change and anthropogenic impacts on population dynamics of hairtail in the East China Sea.

Conserv Physiol 2022 11;10(1):coac044. Epub 2022 Jul 11.

Function Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), 1 Wenhai Road, Aoshanwei, Jimo, Qingdao 266200, People's Republic of China.

The hairtail supports the largest fisheries in the East China Sea. The stock has fluctuated in the past few decades and this variation has been attributed to human pressures and climate change. To investigate energetics of individuals and population dynamics of the species in responses to environmental variations and fishing efforts, we have developed a DEB-IBM by coupling a dynamic energy budget (DEB) model to an individual-based model (IBM). The parameter estimation of DEB model shows an acceptable goodness of fit. The DEB-IBM was validated with histological data for a period of 38 years. High fishing pressure was largely responsible for the dramatic decline of the stock in middle 1980s. The stock recovered from early 1990s, which coincided with introduction of fishing moratorium on spawning stocks in inshore waters and substantial decrease of fishing efforts from large fisheries companies. In addition, the population average age showed a trend of slight decrease. The model successfully reproduced these observations of interannual variations in the population dynamics. The model was then implemented to simulate the effect of climate change on the population performance under greenhouse gas emission scenarios projected for 2100. It was also used to explore population responses to changing fishing mortalities. These scenario simulations have shown that the population biomass under SSP1-1.9, SSP2-4.5 and SSP5-8.5 would decline by 7.5%, 16.6% and 30.1%, respectively, in 2100. The model predicts that increasing fishing mortality by 10% will cause 5.3% decline of the population biomass, whereas decrease of fishing mortality by 10% will result in 6.8% increase of the biomass. The development of the DEB-IBM provides a predictive tool to inform management decisions for sustainable exploitation of the hairtail stock in the East China Sea.
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http://dx.doi.org/10.1093/conphys/coac044DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9273958PMC
July 2022

Relationship between ATOH1 and tumor microenvironment in colon adenocarcinoma patients with different microsatellite instability status.

Cancer Cell Int 2022 Jul 14;22(1):229. Epub 2022 Jul 14.

Department of Oncology, Zhujiang Hospital, Southern Medical University, 253 Industrial Avenue, Guangzhou, 510282, Guangdong, China.

Background: Colon adenocarcinoma (COAD) is one of the major varieties of malignant tumors threatening human health today. Immune checkpoint inhibitors (ICIs) have recently begun to emerge as an effective option for the treatment of COAD patients, but not all patients can benefit from ICI treatment. Previous studies have suggested that ICIs boast significant clinical effects on patients with microsatellite instability-high (MSI-H), while conversely patients with microsatellite-stable/microsatellite instability-low (MSS/MSI-L) have shown limited response.

Methods: We used ATAC-seq, RNA-seq, and mutation data from The Cancer Genome Atlas Colon adenocarcinoma (TCGA-COAD) cohort to perform multi-omics differential analysis on COAD samples with different MSI statuses, then further screened genes by additionally combining these results with survival analysis. We analyzed the effects of the screened genes on the tumor microenvironment and immunogenicity of COAD patients, and subsequently determined their influence on the efficacy of ICIs in COAD patients using a series of predictive indexes.

Results: Twelve genes were screened in the TCGA-COAD cohort, and after the combined survival analysis, we identified ATOH1 as having significant effects. ATOH1 is characterized by high chromatin accessibility, high expression, and high mutation in COAD patients in the MSI-H group. COAD patients with high ATOH1 expression are associated with a better prognosis, unique immune microenvironment, and higher efficacy in ICI treatment. Enrichment analysis showed that COAD patients with high ATOH1 expression displayed significant upregulation in their humoral immunity and other related pathways.

Conclusions: We speculate that ATOH1 may influence the efficacy of ICIs therapy in patients with COAD by affecting the immune microenvironment and immunogenicity of the tumor.
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http://dx.doi.org/10.1186/s12935-022-02651-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9281179PMC
July 2022
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